CN109806618A - A kind of integrated form is adjustable Multi-channel solid phase extraction device and its application method - Google Patents

A kind of integrated form is adjustable Multi-channel solid phase extraction device and its application method Download PDF

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Publication number
CN109806618A
CN109806618A CN201910266404.8A CN201910266404A CN109806618A CN 109806618 A CN109806618 A CN 109806618A CN 201910266404 A CN201910266404 A CN 201910266404A CN 109806618 A CN109806618 A CN 109806618A
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extraction
cells
solid phase
tube
phase extraction
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CN109806618B (en
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王竹青
付大友
袁东
谭文渊
郭雪琰
谭浩兰
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Sichuan University of Science and Engineering
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Sichuan University of Science and Engineering
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Abstract

The invention discloses a kind of adjustable Multi-channel solid phase extraction devices of integrated form, the solid-phase extraction device includes main body, pumping cells, power supply unit, pressure sensing and adjusting unit and extraction cells, several extraction cells connectors for being provided with gas channels in the main body and being connected to by the gas channels with pumping cells, the pressure sensing and adjusting unit are connected with gas channels, the power supply unit is electrically connected with steam extraction unit and pressure sensing and adjusting unit, wherein, sealing control assembly is provided on extraction cells connector, the extraction cells can be mounted on the extraction cells connector and the extraction cells is made to enter Solid Phase Extraction working condition.The invention also discloses the application methods of above-mentioned apparatus.The present invention solves bulky, rate of extraction present in existing Multi-channel solid phase extraction device and adjusts the problems such as slow, the leakproofness difference of reaction, has many advantages, such as small in size, structure is simple, integrated level is high, is quick on the draw, is easy to use.

Description

A kind of integrated form is adjustable Multi-channel solid phase extraction device and its application method
Technical field
The present invention relates to the technical fields of Solid Phase Extraction, more particularly, are related to a kind of adjustable multichannel solid phase of integrated form Extraction equipment and its application method.
Background technique
Existing Multi-channel solid phase extraction device, it is common the technical solution adopted is that: the cavity of a sealing is provided, will be more A test tube collected for extraction liquids etc. is placed in seal cavity using the fixed device such as reagent rack, is received in each liquid The top for collecting test tube is arranged a penetration type connector and is connect by the upper cover plate of seal cavity with extraction bottom of the tube.When in use, It is evacuated from seal cavity using a matched aspiration pump, reaches a negative pressure state, the liquid in extracting tube is outside Under the action of boundary's air pressure by extract layer be pressed into seal cavity and be collected in corresponding test tube.
In the prior art scheme, in order to achieve the purpose that multichannel, device itself is needed using larger space Seal case provides a cavity to place multiple test tubes and its fixed rack for test tube.It is this to design band in actual use Carry out problems: seal cavity is excessive, then the aspiration pump for needing to be equipped with a high-power big flow vacuumizes it, due to Cavity is big, and buffer function is also big, long the time required to vacuumizing, and is difficult fast and accurately to adjust its pressure;Due to seal cavity It is fixed from according to actual use situation and changes, in the less application in some extraction channels, such as when single channel extraction, There is still a need for complete to cause the serious wasting of resources to the pumping process of entire seal cavity;Since all liq collects test tube It is both placed in the same seal cavity, in multichannel extraction application, extract liquor easily flies at fluid connection lower port The phenomenon that splashing causes the mutual pollution between different extraction channels;In addition, since seal cavity is larger, sealing cover and main body it Between be not easy seal, be easy to appear gas leak phenomenon, and be difficult to be integrated with aspiration pump and exterior line etc., operate with It is inconvenient.
Therefore, it is necessary to provide a kind of solid-phase extraction device of optimization.
Summary of the invention
In order to solve the problems in the existing technology, the object of the present invention is to provide one kind with small in size, structure is simple It is single, integrated level is high, the adjustable Multi-channel solid phase extraction device of integrated form for many advantages, such as being quick on the draw, is easy to use and its use Method.
An aspect of of the present present invention provides a kind of adjustable Multi-channel solid phase extraction device of integrated form, the solid-phase extraction device Including main body, pumping cells, power supply unit, pressure sensing and unit and extraction cells are adjusted, are provided with gas circuit in the main body Channel and several extraction cells connectors being connected to by the gas channels with pumping cells, the pressure sensing and adjusting Unit is connected with gas channels, and the power supply unit is electrically connected with steam extraction unit and pressure sensing and adjusting unit, wherein extraction It takes and is provided with sealing control assembly on unit connector, the extraction cells can be mounted on the extraction cells connector simultaneously The extraction cells are made to enter Solid Phase Extraction working condition.
One embodiment of the adjustable Multi-channel solid phase extraction device of integrated form according to the present invention, the sealing control assembly are Seal control valve or self sealing structure, wherein the self sealing structure includes sphere retention groove, cylindrical boss, ball sealer and envelope Spring is closed, the sphere retention groove is arranged in the oral area of extraction cells connector and has extraction cells insert port, the column The top of sphere retention groove is arranged in shape boss, and the closing spring is placed in the bottom of sphere retention groove, and the ball sealer is located at It in sphere retention groove and is placed on closing spring, wherein the closing spring is in pre-compressed state and energy after merging Enough jack up ball sealer when being not inserted into extraction cells blocks the extraction cells insert port realization self-sealing.
One embodiment of the adjustable Multi-channel solid phase extraction device of integrated form according to the present invention, the extraction cells include extraction Pipe, collector tube and standard test tube are taken, back taper interface is provided at the top of the collector tube and bottom is provided with conducting interface, it is described Standard test tube is placed in collector tube, the liquid outlet of the extracting tube be inserted into the back taper interface of the collector tube and with the mark The connection of quasi- test tube, the collector tube inserted into the mouth by the conducting interface insertion extraction cells and with the gas channels Connection.
One embodiment of the adjustable Multi-channel solid phase extraction device of integrated form according to the present invention, outside the bottom of the collector tube Side is additionally provided with annular seal connector, annular seal connector energy after the collector tube is inserted into extraction cells insert port It is enough fastened in extraction cells insert port and realizes sealing.
One embodiment of the adjustable Multi-channel solid phase extraction device of integrated form according to the present invention, in the bottom of the collector tube Side is provided with O-ring seal, and the extraction cells, which insert into the mouth, is also equipped with O-ring seal with the contact position of collector tube.
One embodiment of the adjustable Multi-channel solid phase extraction device of integrated form according to the present invention, the collector tube includes tube body With the lid being arranged at the top of tube body, the lid is connect with tube body by thread seal, and the back taper interface is arranged described At the center upper portion position of lid;The bottom of the tube body is provided with several support columns, several described support columns are along week To being evenly arranged.
One embodiment of the adjustable Multi-channel solid phase extraction device of integrated form according to the present invention, the lower central of the lid Liquid guide flow pipe is provided at position, liquid guide flow pipe lower end is provided with replaceable extension, the extension insertion To standard test tube, the extracting tube is connected to by the liquid guide flow pipe and extension with standard test tube.
One embodiment of the adjustable Multi-channel solid phase extraction device of integrated form according to the present invention, the power supply unit include electricity Source conversion module and lithium battery module, the pumping cells are diaphragm pump, and the pressure sensing and adjusting unit include that pressure passes Feel module and pressure adjusting module.
One embodiment of the adjustable Multi-channel solid phase extraction device of integrated form according to the present invention, the gas channels include master Gas circuit and several branch gas circuits being connected to main gas circuit, several branch gas circuits are connected with several extraction cells connectors, The pumping cells and pressure sensing and adjusting unit are arranged in main gas circuit.
It is described another aspect provides the application method of the adjustable Multi-channel solid phase extraction device of above-mentioned integrated form Application method the following steps are included:
A, several assembled extraction cells are inserted into respectively in several extraction cells connectors and ensure each connection The sealing at place;
B, it opens power supply unit and controls pumping cells and start to be evacuated, by pressure sensing and adjust unit adjustment extraction list Extracting pressure of the pressure of gas circuit cavity to needed for testing in member;
C, liquid to be extracted is added into extraction cells and carries out Solid Phase Extraction experiment, close power supply unit after the completion and takes out extraction Take unit.
Compared with prior art, the adjustable Multi-channel solid phase extraction device of integrated form provided by the invention and its application method solution It is many that bulky present in existing Multi-channel solid phase extraction device, rate of extraction of having determined adjusts slow, leakproofness difference of reaction etc. Problem has many advantages, such as small in size, structure is simple, integrated level is high, is quick on the draw, is easy to use.
Detailed description of the invention
Fig. 1 shows the connection knot of the adjustable Multi-channel solid phase extraction device of integrated form according to an exemplary embodiment of the present invention Structure schematic diagram.
It is single Fig. 2 shows being extracted in the adjustable Multi-channel solid phase extraction device of integrated form according to an exemplary embodiment of the present invention The concrete structure schematic diagram of member insertion extraction cells insert port.
Description of symbols:
1- main body, 11- gas channels, the main gas circuit of 111-, 112- branch gas circuit, 12- extraction cells connector, 121- close bullet Spring, 122- ball sealer, 123- sphere retention groove, 124- cylindrical boss;
2- power supply unit, 21- power conversion module, 22- lithium battery module;
3- pumping cells;
4- pressure sensing and adjusting unit, 41- pressure sensor block, 42- pressure adjusting module;
5- extraction cells, 51- extracting tube, 52- collector tube, 521- lid, 522- tube body, 523- support column, 524- back taper Interface, 528- annular seal connector, 53- standard test tube is connected in interface, 525- liquid guide flow pipe, 526- extension, 527-.
Specific embodiment
All features disclosed in this specification or disclosed all methods or in the process the step of, in addition to mutually exclusive Feature and/or step other than, can combine in any way.
Any feature disclosed in this specification unless specifically stated can be equivalent or with similar purpose by other Alternative features are replaced.That is, unless specifically stated, each feature is an example in a series of equivalent or similar characteristics ?.
First the adjustable Multi-channel solid phase extraction device of integrated form of the invention is specifically described in conjunction with attached drawing below.
Fig. 1 shows the connection knot of the adjustable Multi-channel solid phase extraction device of integrated form according to an exemplary embodiment of the present invention Structure schematic diagram.
As shown in Figure 1, an exemplary embodiment of the present invention, the adjustable Multi-channel solid phase extraction device packet of integrated form It includes main body 1, pumping cells 3, power supply unit 2, pressure sensing and adjusting unit 4 and extraction cells 5, main body 1 is the main of device Bearing function component, pumping cells 3 be used for be evacuated realize experiment when negative pressure state, pressure sensing and adjust unit 4 be used for pair Negative pressure in experimentation is monitored and adjusts, and extraction cells 5 are to realize the primary clustering of Solid Phase Extraction, and power supply unit 2 is then For powering to steam extraction unit 3, pressure sensing and adjusting unit 4.
Specifically, gas channels 11 are provided in main body 1 and are connected to by gas channels 11 with pumping cells 3 several Extraction cells connector 12, pressure sensing and adjusts unit 4 and is connected with gas channels 11, power supply unit 2 and steam extraction unit 3 and Pressure sensing and adjusting unit 4 are electrically connected.
Gas channels 11 of the invention include main gas circuit 111 and several branch gas circuits 112 being connected to main gas circuit 111, if Dry item branch gas circuit 112 is connected with several extraction cells connectors 12, and Solid Phase Extraction experiment can be carried out respectively by being consequently formed Multichannel.Wherein, pumping cells 3 and pressure sensing and adjusting unit 4 are arranged in main gas circuit 111, to realize effective take out Gas and pressure sensing, adjusting.
Wherein, the power supply unit 2 in the present invention includes power conversion module 21 and lithium battery module 22, indoors under environment 220V alternating current can be converted to DC voltage power supply for device when use, lithium battery can be used in an outdoor environment It powers and is convenient for carrying out Solid Phase Extraction experiment in field.
Pumping cells in the present invention can be diaphragm pump, preferably Miniature diaphragm pump.Thus, it is possible to reduce body as far as possible Under the premise of long-pending and power consumption, the vacuum degree of device is improved to the maximum extent, wherein the pumping end of diaphragm pump and gas channels 11 Main gas circuit is connected.
Pressure sensing and adjusting unit 4 in the present invention include pressure sensor block 41 and pressure adjusting module 42, pressure The sensing port of sensing module 41 is connected with the main gas circuit of gas channels 11, with real-time detection and can show the pressure in gas channels Force data.Pressure adjusting module 42 is preferably miniature needle-valve, is also connected to gas channels.As a result, in the worked of unit 3 of sobbing Cheng Zhong can adjust the gas pressure in gas channels by adjusting the opening and closing degree of the miniature needle-valve, to obtain difference Rate of extraction, realize it is adjustable.
Since solid-phase extraction device of the invention is integrated form Multi-channel solid phase extraction equipment, it is therefore desirable to guarantee each channel Independent leakproofness, it is ensured that still be able to reach higher vacuum degree when being tested using some or certain several channels.By This, is additionally provided with sealing control assembly on extraction cells connector 12 of the invention, extraction cells 5 can be mounted on extraction cells On connector 12 and extraction cells 5 is made to enter Solid Phase Extraction working condition, to carry out solid phase extraction manipulations.
According to the present invention, the sealing control assembly that the present invention uses can be seal control valve or self sealing structure.
When using seal control valve, a seal control valve can be set on each extraction cells connector 12, When needing to be tested using the extraction cells connector 12 and corresponding gas channels, then corresponding seal control valve is opened And it controls other corresponding seal control valves without experiment and closes.But the structure and control mode be there are certain defect, by This takes unit connector 12 with self-enclosed structure the present invention also provides another.
It is single Fig. 2 shows being extracted in the adjustable Multi-channel solid phase extraction device of integrated form according to an exemplary embodiment of the present invention The concrete structure schematic diagram of member insertion extraction cells insert port.
As shown in Fig. 2, an exemplary embodiment of the present invention, shown self sealing structure includes sphere retention groove 123, column The oral area of extraction cells connector 12 is arranged in simultaneously in shape boss 124, ball sealer 122 and closing spring 121, sphere retention groove 123 And there is extraction cells insert port, the top of sphere retention groove 123 is arranged in cylindrical boss 124, and closing spring 121 is placed in sphere The bottom of retention groove 123, ball sealer 122 are located in sphere retention groove 123 and are placed on closing spring 121.Due to ball sealer 122 and closing spring 121 be placed in the sphere retention groove 123 with extraction cells insert port, can be realized in slot pair The sealing or unlatching of extraction cells insert port, without departing from sphere retention groove.Wherein, need to ensure to close spring 121 be placed in It is in pre-compressed state afterwards and can jack up ball sealer 122 when being not inserted into extraction cells 5 and blocks extraction cells insert port Realize self-sealing.
In order to it is above-mentioned have the function of seal control assembly apparatus structure and be adapted, the present invention also provides a kind of extractions Take unit 5.Extraction cells 5 of the invention include extracting tube 51, collector tube 52 and standard test tube 53, and the top of collector tube 52 is arranged There is back taper interface 524 and bottom is provided with conducting interface 527, standard test tube 53 is placed in collector tube 52, wherein back taper interface 524 for the mating connection of extracting tube 51, be connected interface 527 be used for the mating connection of extraction cells connector, in extracting tube 51 It is provided with the filler selected according to extraction demand.
It is connected in the back taper interface 524 of the liquid outlet insertion collector tube 52 of extracting tube 51 and with standard test tube 53, collector tube 52 are inserted into extraction cells insert port 12 by conducting interface 527 and are connected to gas channels 11.
When extraction cells 5 are installed on corresponding extraction cells connector 12 in main body 1, especially it is in sealing control assembly When above-mentioned self sealing structure.Under the action of interface 527 are connected in collector tube lower end, closing spring 121 is compressed, ball sealer 122 Decline therewith, then the gas flow path of the extraction cells connector 12 is opened and is connected with the branch gas circuit of main body and main gas circuit.This When, which will be in running order.
When extraction cells 5 are removed from corresponding extraction cells connector 12, work of the ball sealer 121 in closing spring 121 With lower rising and extraction cells insert port is blocked, realizes the gas flow path sealing of the extraction cells connector 12.That is, reducing The application of port number, the extraction cells connector 12 not accessed can be automatically closed, be equivalent to and cut off from gas channels The corresponding cavity spaces generated of extra extraction cells.
As a result, be based on above-mentioned design, apparatus of the present invention can according to the port number of the random regulating device of user demand, and When port number changes, extraction vacuum cavity space corresponding to unit extraction cells is held essentially constant, in this way can be most The effective use of the increase pumping cells such as diaphragm pump of limits improves response speed and pressure adjustment speed.
In order to optimize function, the collector tube 52 of extraction cells 5 includes that tube body 522 and setting are pushed up in tube body 522 in the present invention The lid 521 in portion, lid 521 are connect with tube body 522 by thread seal, can guarantee sealing effect when in use.Back taper connects Mouth 524 is arranged at the center upper portion position of lid, which is the back taper interface of standard, can be with the extraction of standard Take the direct Butt sealing installation of pipe.
Downward liquid guide flow pipe 525 is provided at the lower central position of lid 521,525 lower end of liquid guide flow pipe is set It is equipped with replaceable extension 526, extension 526 is inserted into standard test tube 53, and extracting tube 51 passes through 525 He of liquid guide flow pipe Extension 526 is connected to standard test tube 53.Since extension 526 can be inserted into standard test tube 53, can prevent from extracting Liquid generates splashing in the nozzle of liquid guide flow pipe when taking fast speed and the standard test tube 53 that flies out enters rear end gas circuit to rear end Gas channels generate the case where pollution.
522 bottom of tube body of collector tube 52 is provided with several support columns 523, several support columns 523 are circumferentially It is evenly arranged.On the one hand, in use, the bottom for the standard test tube 53 being put into can lift under the action of support column 523 It rises, its bottom is prevented directly to be pressed on the conducting interface of liquid collecting bottom of the tube and cause airway blockage;On the other hand, it is ensured that Standard test tube 53 is propped up and will not generate big tilt angle, at the same prevented also from standard test tube 53 be stuck in support column 523 with Between the tube wall of collector tube 52.
In order to reinforce sealing effect, the bottom outside of collector tube 52 is additionally provided with annular seal connector 528, and the ring-type is close Envelope connector 528 can be fastened in extraction cells insert port after collector tube 52 is inserted into extraction cells insert port and realize sealing, It can specifically cooperate with cylindrical boss 124 and realize sealing.In addition, the bottom inside of collector tube 52 is provided with O-ring seal, extraction It takes unit to insert into the mouth and is also equipped with O-ring seal with the contact position of collector tube, to guarantee effectively sealing.
Extraction cells of the invention can also use other structures, but as long as can with multichannel, sealable master Body and corresponding extraction cells connector, which match, realizes Solid Phase Extraction function.
The present invention also provides the application methods of the adjustable Multi-channel solid phase extraction device of above-mentioned integrated form, specifically include following Step:
A, several assembled extraction cells are inserted into respectively in several extraction cells connectors and ensure each connection The sealing at place.Wherein, be mainly ensured that extraction cells collector tube and extracting tube back taper joint connection sealing.
B, it opens power supply unit and controls pumping cells and start to be evacuated, by pressure sensing and adjust unit adjustment extraction list Extracting pressure of the pressure of gas circuit cavity to needed for testing in member.Wherein, airline pressure passes through pressure sensor block real-time display, Extracting pressure of the airline pressure to needed for testing can be adjusted in experimentation by pressure adjusting module.
C, liquid to be extracted is added into extraction cells and carries out Solid Phase Extraction experiment, close power supply unit after the completion and takes out extraction Take unit.Wherein, liquid to be extracted is extracted out of extracting tube under differential pressure action and passes through liquid guide flow pipe 525 and extension 526 are collected into standard test tube, complete extraction experiments.
In experimentation, chamber pressure can be adjusted in real time as needed to realize different rate of extraction, can also passed through Extraction cells are plugged to realize the extraction experiments in different channels, extraction cells are removed after the completion of experiment.When using with certainly When the extraction cells connector of sealing structure, even without adjustment seal control valve, extraction cells are connected after removing extraction cells Self-sealing can be realized in mouth.
In conclusion the adjustable Multi-channel solid phase extraction device of integrated form of the invention overcomes existing Multi-channel solid phase extraction Technological deficiency present in device, it is many excellent to have that small in size, structure is simple, integrated level is high, is quick on the draw, is easy to use etc. Point, easy to operate and experiment effect are preferable.
The invention is not limited to specific embodiments above-mentioned.The present invention, which expands to, any in the present specification to be disclosed New feature or any new combination, and disclose any new method or process the step of or any new combination.

Claims (10)

1. a kind of adjustable Multi-channel solid phase extraction device of integrated form, which is characterized in that the solid-phase extraction device includes main body, takes out Gas unit, power supply unit, pressure sensing and unit and extraction cells are adjusted, is provided with gas channels in the main body and passes through institute It states several extraction cells connectors that gas channels are connected to pumping cells, the pressure sensing and adjusting unit and gas circuit is logical Road is connected, and the power supply unit is electrically connected with steam extraction unit and pressure sensing and adjusting unit, wherein extraction cells connector On be provided with sealing control assembly, the extraction cells can be mounted on the extraction cells connector and keep the extraction single Member enters Solid Phase Extraction working condition.
2. the adjustable Multi-channel solid phase extraction device of integrated form according to claim 1, which is characterized in that the sealing control Component is seal control valve or self sealing structure, wherein the self sealing structure includes sphere retention groove, cylindrical boss, sealing Ball and closing spring, the sphere retention groove are arranged in the oral area of extraction cells connector and have extraction cells insert port, The top of sphere retention groove is arranged in the cylindrical boss, and the closing spring is placed in the bottom of sphere retention groove, the sealing Ball is located in sphere retention groove and is placed on closing spring, wherein the closing spring is in pre-compressed state after merging And ball sealer can be jacked up when being not inserted into extraction cells and block the extraction cells insert port realization self-sealing.
3. the adjustable Multi-channel solid phase extraction device of integrated form according to claim 1, which is characterized in that the extraction cells Including extracting tube, collector tube and standard test tube, it is provided with back taper interface at the top of the collector tube and bottom is provided with conducting and connects Mouthful, the standard test tube is placed in collector tube, and the liquid outlet of the extracting tube is inserted into the back taper interface of the collector tube simultaneously Be connected to the standard test tube, the collector tube by the conducting interface be inserted into the extraction cells insert into the mouth and with it is described Gas channels connection.
4. the adjustable Multi-channel solid phase extraction device of integrated form according to claim 3, which is characterized in that the collector tube Bottom outside is additionally provided with annular seal connector, and the annular seal connector is in collector tube insertion extraction cells insertion It can be fastened on after mouthful in extraction cells insert port and realize sealing.
5. the adjustable Multi-channel solid phase extraction device of integrated form according to claim 3, which is characterized in that the collector tube Bottom inside is provided with O-ring seal, and the extraction cells, which insert into the mouth, is also equipped with O-shaped sealing with the contact position of collector tube Circle.
6. the adjustable Multi-channel solid phase extraction device of integrated form according to claim 3, which is characterized in that the collector tube packet The lid for including tube body and being arranged at the top of tube body, the lid are connect with tube body by thread seal, the back taper interface setting At the center upper portion position of the lid;The bottom of the tube body is provided with several support columns, several described support columns Circumferentially it is evenly arranged.
7. the adjustable Multi-channel solid phase extraction device of integrated form according to claim 6, which is characterized in that under the lid Portion's central position is provided with liquid guide flow pipe, and liquid guide flow pipe lower end is provided with replaceable extension, the extension Pipe is inserted into standard test tube, and the extracting tube is connected to by the liquid guide flow pipe and extension with standard test tube.
8. the adjustable Multi-channel solid phase extraction device of integrated form according to claim 1, which is characterized in that the power supply unit Including power conversion module and lithium battery module, the pumping cells are diaphragm pump, and the pressure sensing and adjusting unit include Pressure sensor block and pressure adjusting module.
9. the adjustable Multi-channel solid phase extraction device of integrated form according to claim 1, it is characterised in that the gas channels Including main gas circuit and several branch gas circuits being connected to main gas circuit, several branch gas circuits and several extraction cells connectors It is connected, the pumping cells and pressure sensing and adjusting unit are arranged in main gas circuit.
10. the application method of the integrated form as described in any one of claims 1 to 9 is adjustable Multi-channel solid phase extraction device, special Sign is, the application method the following steps are included:
A, several assembled extraction cells are inserted into respectively in several extraction cells connectors and ensure each junction Sealing;
B, it opens power supply unit and controls pumping cells and start to be evacuated, by pressure sensing and adjust in unit adjustment extraction cells Extracting pressure of the pressure of gas circuit cavity to needed for testing;
C, liquid to be extracted is added into extraction cells and carries out Solid Phase Extraction experiment, close power supply unit after the completion and takes out extraction list Member.
CN201910266404.8A 2019-04-03 2019-04-03 Integrated adjustable multi-channel solid phase extraction device and use method thereof Active CN109806618B (en)

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JP2010000506A (en) * 2009-07-31 2010-01-07 Mitsuhiko Tanahashi Extraction method of vegetable-derived extract and extraction apparatus of the same
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Publication number Priority date Publication date Assignee Title
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