CN109797240A - A kind of molecular labeling, primer and its application with wheat stripe rust resisting ospc gene Yr26 close linkage - Google Patents
A kind of molecular labeling, primer and its application with wheat stripe rust resisting ospc gene Yr26 close linkage Download PDFInfo
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Abstract
The invention belongs to molecular biology fields, more particularly to a kind of and molecular labeling, primer and its application of wheat stripe rust resisting ospc gene Yr26 close linkage, the label is located at wheat 1B chromosome 326913074-326914227, its nucleotide sequence is as shown in the SEQ ID NO.1 in sequence table, it is to isolate that molecular labeling WRS1633, which is conducive to molecular labeling described in the foundation of wheat molecular marker assistant breeding system and Yr26 gene, molecular labeling of the invention can it is easy, quickly, be applied to wheat marker assisted selection with high throughput.
Description
Technical field
The invention belongs to molecular biology fields, and in particular to a kind of and wheat stripe rust resisting ospc gene Yr26 close linkage
Molecular labeling, primer and its application.
Background technique
Stripe rust of wheat is to influence the important disease of world wheat safety in production, and the yield of millions of tons is all caused to damage every year
It loses.China once repeatedly occurred national stripe rust and was very popular.Since the 21th century, since highly pathogenic new microspecies continuously emerge
And development, cause northwest, southwestern area of wheat stripe rust of wheat causes disaster year after year, and high-incidence situation is presented.Due to relying primarily on chemistry
Prevention and treatment, pesticide residue are on the rise with environmental pollution, cause to seriously threaten to China's grain-production safety and ecological environment.It cultivates
The lasting and stable wheat breed of disease resistance is the most economic and environmental-friendly measure of continuous and effective control stripe rust.
In 1970s, Liu great Jun academician and professor Chen Peidu etc. utilize tetraploid duckbill wheat γ 80-1 and tuft
Wheat hybridization, then offspring and hexaploid common wheat is repeatedly returned, and therefrom identifies that select wheat-haynaldia villosa 6VS/6AL easy
Position system.Its parental source has " raise No. 5/4/ γ 80-1/ haynaldia villosa of wheat // peaceful wheat No. 6/3/ and raise wheat No. 2 ", which includes
The germplasm such as 92R137,92R89,92R90,92R149, referred to as " 92R series ".Then successively navigate to the anti-white powder on 6VS
Gene Pm 21 and the gene resistant to stripe rust Yr26 on 1BL.Since the good resistance of the translocation line is in the nineties in last century
Be widely used in wheat breeding, breeder using the translocation line successively cultivate interior wheat serial (No. 8-No. 11), Shi Mai 14,
18 disease-resistant wheat new varieties such as 175, Yang Mai 18 in remote.It is more than 60,000,000 mu that these new varieties, which accumulate popularizing area, is small
1,500,000,000 kilograms of (http://www.cutech.edu.cn/cn/gxkj/2013/01/ of wheat accumulation volume increase
1354173454039838.htm)。
Yr26 is initially located on 1B the short arm of a chromosome (1BS) by horse is gradually new etc., and with Xgwm11, Xgwm18 and
Xgwm413 is chain；And Wang Chunmei etc. is determined Yr26 using the cytogenetics special material (nullisomic, Deletion line) of Chinese spring
C-1BL6-0.32 region of the position on 1B chromosome long arm, and developing Yr26 gene both wings label recently is Xwe173
(1.4cM) and Xbarc181 (6.7cM)；The small Juan of post-tensioning it is equal by comparing genomics using wheat est sequence information and rice,
The conllinear regionl development of false bromegrass devises many closer linked markers, depicts more fine genetic map.In addition, other
Researcher finds that its Rust resistance source is big absolutely to " your the agriculture system " investigation of materials cultivated from Guizhou University professor Zhang Qingqin
Part is Yr26；And come from river wheat 42 and its derivative series that CIMMYT " synthetic wheat " is introduced and cultivated by Sichuan academy of agricultural sciences
Material is through researching and analysing, and anti-source comes from YrCH42 (Li et al.2006), and then verified YrCH42 and Yr26 (Yr24) is
The same gene or same allele.
Since Yr26 is widely applied, item rust microspecies are caused variation occur, 2008 for the first time in Sichuan discovery Yr26 poison
Property microspecies V26, V26 microspecies frequency persistently rises in coming years, and largely exists in the area such as northwest, southwest, to China
Wheat grain-production constitutes a threat to.But the kind containing Yr26 gene is planted extensively, can not all be eliminated in a short time, and Yr26
Still good resistance is kept therefore can to pass through Yr26 and other strains the CYR32 and CYR33 that are still within popular status
The mode of phase gene pyramiding improves and extends its disease resistance, and develops that more closely chain molecular labeling seems with Yr26 gene
It is especially urgent.
Summary of the invention
It the molecular labeling that the present invention provides a kind of with wheat stripe rust resisting ospc gene Yr26 close linkage, primer and its answers
With, with it is easy, quickly, be applied to wheat marker assisted selection with high throughput.
The first purpose of the invention is to provide a kind of and wheat stripe rust resisting ospc gene Yr26 close linkage molecular labelings
WRS1633, the label are located at wheat 1B chromosome 326913074-326914227, with wheat stripe rust resisting ospc gene Yr26
Close linkage, nucleotide sequence is as shown in the SEQ ID NO.1 in sequence table.
A second object of the present invention is to provide drawing for the molecular labeling with wheat stripe rust resisting ospc gene Yr26 close linkage
Object, the sequence of the primer are as follows:
Upstream primer WRS1633F is as shown in SEQ ID NO.2 in sequence table；
Downstream primer WRS1633R is as shown in SEQ ID NO.3 in sequence table.
Third object of the present invention is to provide a kind of molecular labeling wheat stripe rust resisting ospc gene Yr26 detect or
Application in wheat assistant breeding.
Fourth object of the present invention is to provide a kind of primer in wheat stripe rust resisting ospc gene Yr26 detection or wheat
Application in assistant breeding.
A kind of method using the primer detection wheat stripe rust resisting ospc gene Yr26 provided by the invention, specifically include with
Lower step:
S1 extracts the genomic DNA of wheat to be detected；
S2 is with upstream primer WRS1633F and downstream primer WRS1633R using the genomic DNA of the wheat as template
Primer carries out PCR amplification；
S3, agarose gel electrophoresis detection, if the product clip size after amplification is 1364bp, wheat to be measured contains
Stripe Rust Resistance Gene Yr26 can't detect 1364 amplified band, then wheat to be measured does not contain Stripe Rust Resistance Gene Yr26.
Preferably, in the method using the primer detection wheat stripe rust resisting ospc gene Yr26:
The PCR system are as follows: the MgCl of buffer 1.5ul, 25 μM/the μ l of 10 × Baffer2The 4 of 0.9ul, 2.5mM/ μ l
Kind each 0.2ul of dNTP mixture, the Taq enzyme 0.15ul of WRS1633R and WRS1633F each 1.0ul, 5U/ the μ l of 10 μM/μ l,
The template DNA 1.5ul of 50ng/ μ l, adds DDH2O to 15ul；
Response procedures: 1) 94 DEG C 5 minutes；2) 94 DEG C 30 seconds, 55 DEG C 1 minute, 72 DEG C 1 minute, started the cycle over from second step,
35 circulations are carried out altogether；3) 72 DEG C 10 minutes.
Due to using the technology described above, make the beneficial effect that has of the present invention be the present invention provides with the anti-item of wheat
The molecular labeling of rust gene Yr26 close linkage, is conducive to molecule described in the foundation of wheat molecular marker assistant breeding system
Label is to isolate with Yr26 gene, and it is auxiliary that molecular labeling of the invention can be applied to easy, quickly, with high throughput wheat molecule
Help breeding.
Detailed description of the invention
Fig. 1, using WRS1633 primer sets to Susceptible parent Avocet S, disease-resistant parent 92R137, F7Recombinant inbred lines group
The amplification electrophoretogram of body, wherein swimming lane 1-10 is respectively Avocet S, 92R137, ARF7-3、ARF7-7、ARF7-12、
ARF7-34、ARF7-2、ARF7-4、ARF7-8、ARF7-25。
Specific embodiment
The present invention is described in detail combined with specific embodiments below, it is to be understood that protection scope of the present invention is not
It is restricted by specific implementation.The method that actual conditions are not specified in the following example, operates usually according to normal condition, not
Dated material source be it is commercially available, due to not being related to inventive point, therefore its step is not described in detail.
The present invention provides a kind of and wheat stripe rust resisting ospc gene Yr26 close linkage molecular labeling WRS1633, the marks
Note is located at wheat 1B chromosome 326913074-326914227, with wheat stripe rust resisting ospc gene Yr26 close linkage, core
Nucleotide sequence is as shown in SEQ ID NO.1.(with the map of more than 20000 F2 generation volume drawings) in genetic linkage maps,
The molecular labeling is isolated with wheat stripe rust resisting ospc gene Yr26, or being greater than in 20000 samples in wheat, has item rust
Contain the molecular labeling in the sample of sick resistance.Wherein, blade does not generate strip rust bacteria spore and has hypersensitive necrosis reaction disease
Shape is stripe rust resistance.
The preparation method of above-mentioned molecular labeling WRS1633 is as follows:
Take that (wheat lines are that 92R137 hybridizes with susceptible materials A vocet S for the Wheat volatiles DNA that tries wheat lines
F afterwards1It is selfed the F generated2The individual with stripe rust resistance in generation), the extracting of Wheat volatiles DNA is referring to conventional molecule
Biologic operation step；Design primer；So that examination Wheat volatiles DNA is that template carries out PCR amplification.
The Primer Source genome that this laboratory carries out early period resurveys sequence to capture sequencing, tests by sanger sequencing
Card.
Upstream primer WRS1633F:5 '-gagaggaaacaagcgagcga-3 ', as shown in SEQ ID NO.2；
Downstream primer WRS1633R:5 '-cttcgtcttgtggcattccg-3 ', as shown in SEQ ID NO.3；
The PCR system are as follows: the MgCl of buffer 1.5ul, 25 μM/the μ l of 10 × Baffer2The 4 of 0.9ul, 2.5mM/ μ l
Kind each 0.2ul of dNTP mixture, the Taq enzyme 0.15ul of WRS1633R and WRS1633F each 1.0ul, 5U/ the μ l of 10 μM/μ l,
The template DNA 1.5ul of 50ng/ μ l, adds DDH2O to 15ul；
Response procedures: 1) 94 DEG C 5 minutes；2) 94 DEG C 30 seconds, 55 DEG C 1 minute, 72 DEG C 1 minute, started the cycle over from second step,
35 circulations are carried out altogether；3) 72 DEG C 10 minutes.
Extension increasing sequence is determined to SEQ ID NO.1, which is wheat stripe rust resisting ospc gene Yr26 close linkage
Molecular labeling WRS1633.
Based on same inventive concept, the present invention also provides in above-mentioned primer wheat stripe rust resisting ospc gene Yr26 detection
Using, specifically includes the following steps:
To be detected the genomic DNA of wheat as template, with primer WRS1633F (as shown in SEQ ID NO.2) and
WRS1633R (as shown in SEQ ID NO.3) is expanded, and amplified production is obtained, by obtained amplified production carry out sequencing and
Gel electrophoresis.
Specific PCR reaction system is shown in Table 1:
Table 1
PCR program are as follows: 1) 94 DEG C 5 minutes；2) 94 DEG C 30 seconds, 55 DEG C 1 minute, 72 DEG C 1 minute, followed since second step
Ring carries out 35 circulations altogether；3) 72 DEG C 10 minutes.
Fig. 1 is WRS1633 primer pair Susceptible parent Avocet S, disease-resistant parent 92R137, F7 recombinant inbred lines
Amplification, swimming lane 1-10 are respectively Avocet S, 92R137, ARF7-3、ARF7-7、ARF7-12、ARF7-34、ARF7-2、
ARF7-4、ARF7-8、ARF7-25.The result shows that WRS1633 can be in disease-resistant parent 92R137 and offspring's family containing Yr26
Successful amplification goes out the PCR product of one section of 1364bp size, and is then not present in Susceptible parent and offspring's family without containing Yr26
Such one section of PCR product illustrates the detection that wheat stripe rust resisting disease resistance can be carried out using molecular labeling WRS1633.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Sequence table
<110>Xibei Univ. of Agricultural & Forest Science & Technology
<120>a kind of molecular labeling, preparation method and its application with wheat stripe rust resisting ospc gene Yr26 close linkage
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1364
<212> DNA
<213>artificial sequence
<400> 1
gagaggaaac aagcgagcga tcggctgaca ctgacaaaat gagaaaggaa gaaacaaata 60
aaagatctga gaaaataatc gatggtatgt ggcacatcca agaacaggtg gtaccaaata 120
tatacataat gaaatttcta gcttttattc aacatagaat ttagtactag tttccgaatc 180
tttggtgcag catgattaca aaaacctgtt tctattgaaa ctggaatgcc tacgcatatc 240
tgtaaataaa atagaagtac agttctttgg ttgtataatg ctaaactgtc cagttaaaat 300
atttgaagga ccttgccttg cacaaagaac tggccatcac taagttccta aagtgagtag 360
gtacggtgag ctaaaggata catataagct aaaatgttgg ataacgcatg catgccattt 420
atataaatca tttgtgatgt aactgcctaa ctggtaatag gtgcatacca tttatgccca 480
ccaacttcaa atgtagggga acggacaaag tgatccaagt ccagttcaag gaaattgttc 540
atgttccaag tgtatgtccc tttgacgaac tccttcttct gaacaaagag gttctgaact 600
gtcgtaggct tcttctgaac cacaacagcc ttcttttcag gagaagagac atcaattttt 660
aatatctcca caccgaagac acagctatca tcaactagaa aagcagatga tttcagtagc 720
tcctgaagag gaatcaagca atgctccttc gagaagatat tcttgaaacc aaagttgtag 780
ctagctgcac ttaggtaaag gttagaactt ggaagattag acagcgaaga acttgtatgc 840
aagaagcaaa tattaccaaa taatatctaa ctaaccttca ccctagttaa gaaagcaagg 900
tagtgaatta catccagatg atgataatca aatttaaaga gaaacatgcg tatgctatct 960
gaaccataat agactcaagt gtgaaacttg taatgtctca agtgagatac atgtgtatgc 1020
catcagaacc atccaaattc ctgaaggtct acagagaacc ttgacagcaa agcaaataca 1080
acgcgacttg ttaatgccaa aacattgtac ttgaccatat tttgatattt acagatattg 1140
acttgtaatg atagaatcat ggtatttata tctcaaactt atgacacaca gaggagtatg 1200
gattcatagc tataatatga tggacgtgct tgaaaaaaca catccatgag actacctttg 1260
catccacagt acattcgtct tgaatggttg tatattgaca actcaaacac cggatgcacc 1320
atgtgacctg gcaccaagct tggtcggaat gccacaagac gaag 1364
<210> 2
<211> 20
<212> DNA
<213>artificial sequence
<400> 2
gagaggaaac aagcgagcga 20
<210> 3
<211> 20
<212> DNA
<213>artificial sequence
<400> 3
cttcgtcttg tggcattccg 20
Claims (6)
1. a kind of with wheat stripe rust resisting ospc gene Yr26 close linkage molecular labeling, which is characterized in that the molecular labeling is
WRS1633, the label are located at wheat 1B chromosome 326913074-326914227, nucleotide sequence such as SEQ ID
Shown in NO.1.
2. a kind of primer with the molecular labeling of wheat stripe rust resisting ospc gene Yr26 close linkage according to claim 1,
It is characterized in that, the sequence of the primer are as follows:
Upstream primer WRS1633F is as shown in SEQ ID NO.2 in sequence table；
Downstream primer WRS1633R is as shown in SEQ ID NO.3 in sequence table.
3. molecular labeling according to claim 1 is in wheat stripe rust resisting ospc gene Yr26 detection or wheat assistant breeding
Using.
4. primer according to claim 2 answering in wheat stripe rust resisting ospc gene Yr26 detection or wheat assistant breeding
With.
5. application according to claim 4, which is characterized in that the detection of wheat stripe rust resisting ospc gene Yr26 specifically include with
Lower step:
S1 extracts the genomic DNA of wheat to be detected；
S2, using the genomic DNA of the wheat as template, using upstream primer WRS1633F and downstream primer WRS1633R as primer
Carry out PCR amplification；
S3, agarose gel electrophoresis detection, if the product clip size after amplification is 1364bp, wheat to be measured contains anti-item
Rust gene Yr26, can't detect 1364 amplified band, then wheat to be measured does not contain Stripe Rust Resistance Gene Yr26.
6. application according to claim 5, which is characterized in that in S2, the PCR system are as follows: the buffering of 10 × Baffer
The MgCl of liquid 1.5ul, 25 μM/μ l2The WRS1633R of 4 kinds of dNTP mixture each 0.2ul, 10 μM/μ l of 0.9ul, 2.5mM/ μ l
Add DDH with each 1.0ul of WRS1633F, the template DNA 1.5ul of Taq enzyme 0.15ul, the 50ng/ μ l of 5U/ μ l2O to 15ul；
Response procedures: 1) 94 DEG C 5 minutes；2) 94 DEG C 30 seconds, 55 DEG C 1 minute, 72 DEG C 1 minute, started the cycle over from second step, altogether into
Row 35 circulations；3) 72 DEG C 10 minutes.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114410652A (en) * | 2022-01-21 | 2022-04-29 | 西南科技大学 | Stripe rust resistant gene QYRsv and swust-1BL tightly linked molecular marker in adult plant stage and application thereof |
| CN116121444A (en) * | 2023-03-03 | 2023-05-16 | 西北农林科技大学 | Functional KASP molecular marker of wheat stripe rust resistance gene YrNP63 and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101487056A (en) * | 2009-02-26 | 2009-07-22 | 中国农业科学院作物科学研究所 | Method for assistantly screening anti-stripe rust wheat, and special primer therefor |
| CN102618638A (en) * | 2012-01-13 | 2012-08-01 | 山西省农业科学院作物科学研究所 | Molecular marker for wheat stripe rust disease-resistant new gene Yr50 assisted selection and using method thereof |
| CN105132570A (en) * | 2015-09-22 | 2015-12-09 | 中国农业科学院作物科学研究所 | Primer combination assisting in screening stripe-rust-resistant wheat and application of primer combination |
| US20180327775A1 (en) * | 2013-08-21 | 2018-11-15 | Commonwealth Scientific And Industrial Research Organisation | Rust Resistance Gene |
-
2019
- 2019-03-20 CN CN201910214777.0A patent/CN109797240B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101487056A (en) * | 2009-02-26 | 2009-07-22 | 中国农业科学院作物科学研究所 | Method for assistantly screening anti-stripe rust wheat, and special primer therefor |
| CN102618638A (en) * | 2012-01-13 | 2012-08-01 | 山西省农业科学院作物科学研究所 | Molecular marker for wheat stripe rust disease-resistant new gene Yr50 assisted selection and using method thereof |
| US20180327775A1 (en) * | 2013-08-21 | 2018-11-15 | Commonwealth Scientific And Industrial Research Organisation | Rust Resistance Gene |
| CN105132570A (en) * | 2015-09-22 | 2015-12-09 | 中国农业科学院作物科学研究所 | Primer combination assisting in screening stripe-rust-resistant wheat and application of primer combination |
Non-Patent Citations (4)
| Title |
|---|
| CHUNMEI WANG等: "SSR and STS markers for wheat stripe rust resistance gene Yr26", 《EUPHYTICA》 * |
| 吴建辉: "基于BSR-Seq和芯片技术的抗条锈基因Yr26候选基因分析及普通小麦成株期抗条锈QTL定位", 《中国优秀博硕士学位论文全文数据库（博士）农业科技辑》 * |
| 曾庆东: "小麦抗条锈病基因Yr26载体材料BAC文库构建及转录组测序", 《中国优秀博硕士学位论文全文数据库（博士）农业科技辑》 * |
| 邢书娟: "小麦抗条锈病Yr26相关基因的筛选和克隆", 《中国优秀博硕士学位论文全文数据库（硕士）农业科技辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114410652A (en) * | 2022-01-21 | 2022-04-29 | 西南科技大学 | Stripe rust resistant gene QYRsv and swust-1BL tightly linked molecular marker in adult plant stage and application thereof |
| CN116121444A (en) * | 2023-03-03 | 2023-05-16 | 西北农林科技大学 | Functional KASP molecular marker of wheat stripe rust resistance gene YrNP63 and application thereof |
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