CN109793073A - The extracting method at Ilex paraguarensis drink Lipid-lowering activities position and hypoglycemic activity position - Google Patents
The extracting method at Ilex paraguarensis drink Lipid-lowering activities position and hypoglycemic activity position Download PDFInfo
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- CN109793073A CN109793073A CN201910219423.5A CN201910219423A CN109793073A CN 109793073 A CN109793073 A CN 109793073A CN 201910219423 A CN201910219423 A CN 201910219423A CN 109793073 A CN109793073 A CN 109793073A
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Abstract
The invention discloses a kind of extracting methods at Ilex paraguarensis drink Lipid-lowering activities position and hypoglycemic activity position, it is characterized by comprising the following steps: S1: taking Ilex paraguarensis to drink Akg, crush, with 70-90 DEG C of decoction 2.5-3.5h of the 5-15 times of water measured, period stirs frequently, filters to get filtrate, and repeats 70-90 DEG C of decoction 2.5-3.5h of the 5-15 times of water measured, it is concentrated under reduced pressure after merging filtrate, medicinal extract twice, discards petroleum ether with petroleum ether degreasing, obtains Ilex paraguarensis drinking-water extract medicinal extract;S2: being grouped with absorption resin, and successively with 30% ethyl alcohol, 60% ethyl alcohol, 90% ethanol elution, eluent is concentrated under reduced pressure, and obtains Ilex paraguarensis drinking-water extract Y1,30% ethyl alcohol extractive part Y2,60% ethyl alcohol extractive part Y3,90% ethyl alcohol extractive part Y4;S3: concentration, freeze-drying, refrigerator save；S4: optimal Lipid-lowering activities position and hypoglycemic activity position are chosen according to extract experimental result.The invention has the benefit that the extraction process at Lipid-lowering activities position and hypoglycemic activity position is convenient and efficient.
Description
Technical field
The present invention relates to a kind of extracting methods at Ilex paraguarensis drink Lipid-lowering activities position and hypoglycemic activity position.
Background technique
Drink tea has become one of people's life habit, and especially in ancient Chinese culture, tea is
Become a kind of culture.It is also higher and higher for the quality requirements of drink tea with the promotion of people's quality of life, it is such as a large amount of
Tea is biased to health protection tea, slim tea etc., and then improves people's lives quality by these health protection teas, slim tea.
The extracting method process at existing Ilex paraguarensis drink Lipid-lowering activities position and hypoglycemic activity position is complicated, needs further
It improves.
Summary of the invention
The purpose of the present invention is to provide a kind of Ilex paraguarensis drink Lipid-lowering activities position and hypoglycemic activity position extracting method,
Its extraction process is convenient.
To achieve the above object, the technical scheme is that
A kind of extracting method at Ilex paraguarensis drink Lipid-lowering activities position and hypoglycemic activity position, which comprises the steps of:
S1: taking Ilex paraguarensis to drink Akg, crushes, with 70-90 DEG C of decoction 2.5-3.5h of the 5-15 times of water measured, during which stirs frequently, mistake
Filtrate is filtered to obtain, 70-90 DEG C of decoction 2.5-3.5h of the 5-15 times of water measured is repeated, is concentrated under reduced pressure after merging filtrate, medicinal extract petroleum ether
Degreasing twice, discards petroleum ether, obtains Ilex paraguarensis drinking-water extract medicinal extract;
S2: being grouped with absorption resin, and successively with 30% ethyl alcohol, 60% ethyl alcohol, 90% ethanol elution, elution is concentrated under reduced pressure
Liquid obtains Ilex paraguarensis drinking-water extract Y1,30% ethyl alcohol extractive part Y2,60% ethyl alcohol extractive part Y3,90% ethyl alcohol position
Extract Y4;
S3: concentration, freeze-drying, refrigerator save；
S4: optimal Lipid-lowering activities position and hypoglycemic activity position are chosen according to extract experimental result.
Preferably, include the following steps:
S1: taking Ilex paraguarensis to drink 20kg, crushes, and during which the 80 DEG C of decoction 3h of water measured with 10 times are stirred frequently, filtered to get filtrate, weight
80 DEG C of decoction 3h of water of 10 times of amounts are answered, are concentrated under reduced pressure after merging filtrate, medicinal extract twice, discards petroleum ether with petroleum ether degreasing, obtains
Ilex paraguarensis drinking-water extract medicinal extract;
S2: being grouped with absorption resin, successively uses water, 30% ethyl alcohol, 60% ethyl alcohol, 90% ethanol elution, and reduced pressure is washed
De- liquid obtains Ilex paraguarensis drinking-water extract Y1,30% ethyl alcohol extractive part Y2,60% ethyl alcohol extractive part Y3,90% ethyl alcohol portion
Position extract Y4;
S3: concentration, freeze-drying, refrigerator save；
S4: optimal Lipid-lowering activities position and hypoglycemic activity position are chosen according to extract experimental result.
The invention has the benefit that the extraction process at Lipid-lowering activities position and hypoglycemic activity position is convenient and efficient.
Specific embodiment
With reference to embodiment, the specific embodiment of the present invention is further described.Following embodiment is only used for more
Add and clearly demonstrate technical solution of the present invention, and not intended to limit the protection scope of the present invention.
The technical solution that the present invention embodies is:
Embodiment 1
It takes Ilex paraguarensis to drink 20kg, crushes, during which the 80 DEG C of decoction 3h of water measured with 10 times are stirred frequently, filtered to get filtrate, weight
Multiple 10 times of amount boilings are primary, are concentrated under reduced pressure after merging filtrate, medicinal extract twice, discards petroleum ether, get Ma Dai with petroleum ether degreasing
Tea-drinking water extract medicinal extract.Rough segmentation is carried out with D101 type macroporous absorbent resin afterwards, successively with 30% ethyl alcohol, 60% ethyl alcohol, 90%
Eluent is concentrated under reduced pressure in ethanol elution, obtains Ilex paraguarensis drinking-water extract (Y1), 30% ethyl alcohol position (Y2), 60% ethyl alcohol position
(Y3), 90% ethyl alcohol position medicinal extract (Y4).Concentration, freeze-drying, refrigerator save backup.
1 material and instrument
1.1 experimental animals: Kunming male mice, 22 ± 2g of weight.
1.2 drugs and reagent
Ilex paraguarensis drinks the self-control of different parts powder, Y1, Y2, Y3, Y4, simvastatin.Mouse total cholesterol (TC) kit, mouse
Triglycerides (TG) kit, mouse high-density lipoprotein (HDL-C) kit, mouse low-density lipoprotein (LDL-C) reagent
Box, mouse superoxide dismutase (SOD) kit.Ethyl alcohol (analysis is pure), acetonitrile (chromatographically pure), methanol (chromatographically pure), formic acid
(analysis is pure).
1.3 key instrument
Electronic analytical balance, high speed freezing centrifuge, freeze drier, Rotary Evaporators, adjustable electric hot plate, semiautomatic biochemistry
Analyzer, Olympus inverted microscope.
2, method
2.1 model and grouping
It chooses high lipid food nursing method and establishes experimental hyperlipidemia mouse model.KM male mice feeds 3d in advance, when experiment with
Machine picks out 10 mouse as blank control group (K), is fed with chow diet, remaining mouse feeds 2w with high lipid food
Experimental Mice hyperlipemia model is established, the 14d tail vein for giving high lipid food takes hematometry modeling mouse fasting blood
Clear four items of blood lipid tests is horizontal.The successful hyperlipemia in mice of modeling 60 is taken, is randomly divided into 6 groups, every group 10.It is followed successively by mould
Type control group (M), Simvastatin positive controls (X), Ilex paraguarensis drinking-water position group (Y1), Ilex paraguarensis drink 30% ethyl alcohol position group
(Y2), Ilex paraguarensis drinks 60% ethyl alcohol position group (Y3), Ilex paraguarensis drinks 90% ethyl alcohol position group (Y4).Each group mouse divides cage to feed,
Free diet alternately illuminates, 23~25 DEG C of temperature, humidity 40%~70% for 12 hours.During experiment, every 4 days weighing each groups
Mouse weight observes each group mouse diet amount, amount of drinking water and activity condition.
2.2 administration modes and nursing
In addition to blank group, Simvastatin group is administered by mouse weight 40mg/kg, and Ilex paraguarensis drinks each position group and presses mouse constitution
1.67/kg administration is measured, model group gives same volume 0.9% physiological saline, successive administration 4w.Two is small after last time is administered
When anesthesia put to death all mouse, eyeball takes blood 1ml.Blood stands 2h, and 5000r/min centrifugation 10min takes serum, and -80 DEG C
Low temperature refrigerator stores serum.After taking blood, each group mouse is dissected, liver, kidney, spleen of each group mouse etc. is won rapidly and carries out disease
Neo-Confucianism checks, it is spare to be stored in -80 DEG C of low temperature refrigerators.Partial liver is taken to be fixed with universal tissue fixative solution simultaneously, it is conventional de-
Slice is made in water, paraffin embedding, and HE is dyed, and observes under inverted microscope.
2.3 detection
The each group mouse state of mind, activity condition, hair color situation, food-intake and drink are observed during experiment
Water, secretion, excreta, body weights etc..
Biochemical reagents box measures the content of TC, LDL-C, TG, HDL-C, SOD in mice serum.
One-way analysis of variance is carried out to each group of data using statistical software, comparison is examined using t between group, experimental result
It is indicated with mean ± SD, p < 0.05 is to have significant difference, has statistical significance.
Histopathological examination and the observation of HE hepatic pathology section.
2.4 analyze that with pathological examination to show that Ilex paraguarensis drinks a part (X) blood fat reducing function optimal through data.
Embodiment 2
It takes Ilex paraguarensis to drink 20kg, crushes, during which the 80 DEG C of decoction 3h of water measured with 10 times are stirred frequently, filtered to get filtrate, weight
Multiple 10 times of amount boilings are primary, are concentrated under reduced pressure after merging filtrate, medicinal extract twice, discards petroleum ether, get Ma Dai with petroleum ether degreasing
Tea-drinking water extract medicinal extract.Rough segmentation is carried out with D101 type macroporous absorbent resin afterwards, successively with 30% ethyl alcohol, 60% ethyl alcohol, 90%
Eluent is concentrated under reduced pressure in ethanol elution, obtains Ilex paraguarensis drinking-water extract (Y1), 30% ethyl alcohol position (Y2), 60% ethyl alcohol position
(Y3), 90% ethyl alcohol position medicinal extract (Y4).Concentration, freeze-drying, refrigerator save backup.
1, material and instrument
1.1 experimental animals: Kunming male mice, 22 ± 2g of weight.
1.2 drugs and reagent
Ilex paraguarensis drinks the self-control of different parts powder, Y1, Y2, Y3, Y4, streptozotocin (STZ), melbine, acetonitrile (chromatography
It is pure), methanol (chromatographically pure), remaining reagent is that analysis is pure.
1.3 key instrument
Electronic analytical balance, blood-sugar detecting instrument, eight lead physiograph, camera.
2. method
2.1 models and grouping
STZ is before use in ice bath with the preparation of 0.1mol/L sodium citrate buffer, and miillpore filter filtration sterilizes, and ip gives
Rat 60 mg/kg1 times takes tail hematometry blood glucose level after 72 h, and blood glucose value >=16.5 mmol/L are as diabetes rat
The standard of Cheng Mo.Successful 60 diabetes rats of modeling are randomly divided into 6 groups: model group (M), the melbine positive are right
30% ethyl alcohol position group (Y2) is drunk according to group (G), Ilex paraguarensis drinking-water position group (Y1), Ilex paraguarensis, Ilex paraguarensis drinks 60% ethyl alcohol position
Group (Y3), Ilex paraguarensis drink 90% ethyl alcohol position group (Y4), and normal rat 10 is separately taken to be only used as blank control group (K).
2.2 administration modes and nursing
Each equal ig administration of administration group rat, melbine positive controls (0.15g/kg), remaining Ilex paraguarensis drink each position group
(1g/kg) daily 1 time, continues 11 weeks, blank group and model group give equal amount of distilled water.It was surveyed in administration the 6th, 11 week
Fixed 24 h glucoses in urine and blood glucose level.The urine sample for acquiring 24 h collection measures urine sugar value using professional test paper.Big rat-tail is quiet
Arteries and veins takes blood, and blood glucose meter and test paper detect blood glucose level.25 mg/kg of rat ip yellow Jackets is anaesthetized after being administered 11 weeks,
It faces upward position to fix, separates exposure right carotid, ligature distal end, proximal part artery clamp folder closes, pressure sensor is inserted into
Physiograph is led in left ventricle, connection eight, detects left ventricular mean systolic blood pressure (LVSP), left ventricle
Press (LVEDP), average Left Ventricular internal pressure maximum climbing speed (+dp/dtmax) and maximum fall off rate (- dp/ in mean diastolic blood end
), and synchronous recording heart rate dtmax.
2.3 detection
The each group mouse state of mind, activity condition, hair color situation, food-intake and drink are observed during experiment
Water, secretion, excreta, body weights etc..
One-way analysis of variance is carried out to each group of data using statistical software, comparison is examined using t between group, experimental result
It is indicated with mean ± SD, p < 0.05 is to have significant difference, has statistical significance.
2.4 analyze that with pathological examination to show that Ilex paraguarensis drinks a part (X) function of reducing blood sugar optimal through data.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, without departing from the technical principles of the invention, several improvements and modifications can also be made, these improvements and modifications
Also it should be regarded as protection scope of the present invention.
Claims (2)
1. the extracting method at a kind of Ilex paraguarensis drink Lipid-lowering activities position and hypoglycemic activity position, which is characterized in that including walking as follows
It is rapid:
S1: taking Ilex paraguarensis to drink Akg, crushes, with 70-90 DEG C of decoction 2.5-3.5h of the 5-15 times of water measured, during which stirs frequently, mistake
Filtrate is filtered to obtain, 70-90 DEG C of decoction 2.5-3.5h of the 5-15 times of water measured is repeated, is concentrated under reduced pressure after merging filtrate, medicinal extract petroleum ether
Degreasing twice, discards petroleum ether, obtains Ilex paraguarensis drinking-water extract medicinal extract;
S2: being grouped with absorption resin, and successively with 30% ethyl alcohol, 60% ethyl alcohol, 90% ethanol elution, elution is concentrated under reduced pressure
Liquid obtains Ilex paraguarensis drinking-water extract Y1,30% ethyl alcohol extractive part Y2,60% ethyl alcohol extractive part Y3,90% ethyl alcohol position
Extract Y4;
S3: concentration, freeze-drying, refrigerator save；
S4: optimal Lipid-lowering activities position and hypoglycemic activity position are chosen according to extract experimental result.
2. the extracting method at Ilex paraguarensis drink according to claim 1 Lipid-lowering activities position and hypoglycemic activity position, feature
It is, includes the following steps:
S1: taking Ilex paraguarensis to drink 20kg, crushes, and during which the 80 DEG C of decoction 3h of water measured with 10 times are stirred frequently, filtered to get filtrate, weight
80 DEG C of decoction 3h of water of 10 times of amounts are answered, are concentrated under reduced pressure after merging filtrate, medicinal extract twice, discards petroleum ether with petroleum ether degreasing, obtains
Ilex paraguarensis drinking-water extract medicinal extract;
S2: being grouped with absorption resin, successively uses water, 30% ethyl alcohol, 60% ethyl alcohol, 90% ethanol elution, and reduced pressure is washed
De- liquid obtains Ilex paraguarensis drinking-water extract Y1,30% ethyl alcohol extractive part Y2,60% ethyl alcohol extractive part Y3,90% ethyl alcohol portion
Position extract Y4;
S3: concentration, freeze-drying, refrigerator save；
S4: optimal Lipid-lowering activities position and hypoglycemic activity position are chosen according to extract experimental result.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115736061A (en) * | 2022-10-21 | 2023-03-07 | 宁波绿之健药业有限公司 | Preparation process of mate tea extract |
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| CN1569120A (en) * | 2004-04-28 | 2005-01-26 | 江苏中康新药指纹图谱开发有限公司 | Effective portion extraction of dogwood and its preparation method and usage |
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| CN115736061A (en) * | 2022-10-21 | 2023-03-07 | 宁波绿之健药业有限公司 | Preparation process of mate tea extract |
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Application publication date: 20190524 |