CN109790551A - 改进的基因编辑 - Google Patents

改进的基因编辑 Download PDF

Info

Publication number
CN109790551A
CN109790551A CN201780050339.4A CN201780050339A CN109790551A CN 109790551 A CN109790551 A CN 109790551A CN 201780050339 A CN201780050339 A CN 201780050339A CN 109790551 A CN109790551 A CN 109790551A
Authority
CN
China
Prior art keywords
sequence
nucleic acid
recombinase
cell
genome
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201780050339.4A
Other languages
English (en)
Chinese (zh)
Inventor
亚当·布赖恩·罗伯逊
朱莉娅·罗伯逊
阿妮卡·赖夫施奈德
阿尔内·克伦格兰
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oslo Universitetssykehus hf
Original Assignee
Oslo Universitetssykehus hf
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oslo Universitetssykehus hf filed Critical Oslo Universitetssykehus hf
Publication of CN109790551A publication Critical patent/CN109790551A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN201780050339.4A 2016-06-16 2017-06-16 改进的基因编辑 Pending CN109790551A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662350941P 2016-06-16 2016-06-16
US62/350,941 2016-06-16
PCT/IB2017/000967 WO2018229521A1 (en) 2016-06-16 2017-06-16 Improved gene editing

Publications (1)

Publication Number Publication Date
CN109790551A true CN109790551A (zh) 2019-05-21

Family

ID=65019181

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201780050339.4A Pending CN109790551A (zh) 2016-06-16 2017-06-16 改进的基因编辑

Country Status (7)

Country Link
US (2) US11708588B2 (enExample)
EP (1) EP3472329A1 (enExample)
JP (1) JP2020511931A (enExample)
CN (1) CN109790551A (enExample)
CA (1) CA3028074A1 (enExample)
CL (1) CL2018003649A1 (enExample)
WO (1) WO2018229521A1 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111518220A (zh) * 2020-05-14 2020-08-11 重庆英茂盛业生物科技有限公司 一种融合蛋白及其设计方法
CN111909961A (zh) * 2020-08-21 2020-11-10 华侨大学 一种CRISPR/Cas介导的ATL细胞基因编辑载体及其应用
CN112979821A (zh) * 2019-12-18 2021-06-18 华东师范大学 一种提高基因编辑效率的融合蛋白及其应用
CN113891938A (zh) * 2019-05-29 2022-01-04 孟山都技术公司 使用增强的靶向编辑技术的定向基因组工程

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230407339A1 (en) * 2020-11-06 2023-12-21 The University Of Hong Kong Transferable type i-f crispr-cas genome editing system

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1529759A (zh) * 2001-01-10 2004-09-15 模块转染系统
WO2015089351A1 (en) * 2013-12-12 2015-06-18 The Broad Institute Inc. Compositions and methods of use of crispr-cas systems in nucleotide repeat disorders
EP2895620A1 (en) * 2012-09-11 2015-07-22 Life Technologies Corporation Nucleic acid amplification
CN104946621A (zh) * 2014-03-28 2015-09-30 中国科学院上海生命科学研究院 基因敲入组合物及其使用方法和应用
CN105073981A (zh) * 2012-10-30 2015-11-18 重组股份有限公司 动物中性成熟的控制
WO2016028682A1 (en) * 2014-08-17 2016-02-25 The Broad Institute Inc. Genome editing using cas9 nickases
WO2016054326A1 (en) * 2014-10-01 2016-04-07 The General Hospital Corporation Methods for increasing efficiency of nuclease-induced homology-directed repair
US20160153006A1 (en) * 2013-06-17 2016-06-02 The Broad Institute Inc. Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4217344A (en) 1976-06-23 1980-08-12 L'oreal Compositions containing aqueous dispersions of lipid spheres
US4235871A (en) 1978-02-24 1980-11-25 Papahadjopoulos Demetrios P Method of encapsulating biologically active materials in lipid vesicles
US4186183A (en) 1978-03-29 1980-01-29 The United States Of America As Represented By The Secretary Of The Army Liposome carriers in chemotherapy of leishmaniasis
US4261975A (en) 1979-09-19 1981-04-14 Merck & Co., Inc. Viral liposome particle
US4485054A (en) 1982-10-04 1984-11-27 Lipoderm Pharmaceuticals Limited Method of encapsulating biologically active materials in multilamellar lipid vesicles (MLV)
US4501728A (en) 1983-01-06 1985-02-26 Technology Unlimited, Inc. Masking of liposomes from RES recognition
US4946787A (en) 1985-01-07 1990-08-07 Syntex (U.S.A.) Inc. N-(ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US4897355A (en) 1985-01-07 1990-01-30 Syntex (U.S.A.) Inc. N[ω,(ω-1)-dialkyloxy]- and N-[ω,(ω-1)-dialkenyloxy]-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US5049386A (en) 1985-01-07 1991-09-17 Syntex (U.S.A.) Inc. N-ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)Alk-1-YL-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US4797368A (en) 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US4774085A (en) 1985-07-09 1988-09-27 501 Board of Regents, Univ. of Texas Pharmaceutical administration systems containing a mixture of immunomodulators
JP2874751B2 (ja) 1986-04-09 1999-03-24 ジェンザイム・コーポレーション 希望する蛋白質をミルク中へ分泌する遺伝子移植動物
US4837028A (en) 1986-12-24 1989-06-06 Liposome Technology, Inc. Liposomes with enhanced circulation time
US4873316A (en) 1987-06-23 1989-10-10 Biogen, Inc. Isolation of exogenous recombinant proteins from the milk of transgenic mammals
US5264618A (en) 1990-04-19 1993-11-23 Vical, Inc. Cationic lipids for intracellular delivery of biologically active molecules
WO1991017424A1 (en) 1990-05-03 1991-11-14 Vical, Inc. Intracellular delivery of biologically active substances by means of self-assembling lipid complexes
US5173414A (en) 1990-10-30 1992-12-22 Applied Immune Sciences, Inc. Production of recombinant adeno-associated virus vectors
US5587308A (en) 1992-06-02 1996-12-24 The United States Of America As Represented By The Department Of Health & Human Services Modified adeno-associated virus vector capable of expression from a novel promoter
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US7868149B2 (en) 1999-07-20 2011-01-11 Monsanto Technology Llc Plant genome sequence and uses thereof
US6603061B1 (en) 1999-07-29 2003-08-05 Monsanto Company Agrobacterium-mediated plant transformation method
US20090100536A1 (en) 2001-12-04 2009-04-16 Monsanto Company Transgenic plants with enhanced agronomic traits
US8889394B2 (en) 2009-09-07 2014-11-18 Empire Technology Development Llc Multiple domain proteins
US9405700B2 (en) 2010-11-04 2016-08-02 Sonics, Inc. Methods and apparatus for virtualization in an integrated circuit
EP3450563A1 (en) * 2011-02-25 2019-03-06 Recombinetics, Inc. Genetically modified animals and methods for making the same
US20140310830A1 (en) 2012-12-12 2014-10-16 Feng Zhang CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes
US8697359B1 (en) * 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
AU2015342749B2 (en) 2014-11-07 2022-01-27 Editas Medicine, Inc. Methods for improving CRISPR/Cas-mediated genome-editing
EP3274453B1 (en) * 2015-03-26 2021-01-27 Editas Medicine, Inc. Crispr/cas-mediated gene conversion
US9988637B2 (en) * 2015-10-26 2018-06-05 National Tsing Hua Univeristy Cas9 plasmid, genome editing system and method of Escherichia coli

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1529759A (zh) * 2001-01-10 2004-09-15 模块转染系统
EP2895620A1 (en) * 2012-09-11 2015-07-22 Life Technologies Corporation Nucleic acid amplification
CN105073981A (zh) * 2012-10-30 2015-11-18 重组股份有限公司 动物中性成熟的控制
US20160153006A1 (en) * 2013-06-17 2016-06-02 The Broad Institute Inc. Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation
WO2015089351A1 (en) * 2013-12-12 2015-06-18 The Broad Institute Inc. Compositions and methods of use of crispr-cas systems in nucleotide repeat disorders
CN104946621A (zh) * 2014-03-28 2015-09-30 中国科学院上海生命科学研究院 基因敲入组合物及其使用方法和应用
WO2016028682A1 (en) * 2014-08-17 2016-02-25 The Broad Institute Inc. Genome editing using cas9 nickases
WO2016054326A1 (en) * 2014-10-01 2016-04-07 The General Hospital Corporation Methods for increasing efficiency of nuclease-induced homology-directed repair

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
KENAN C. MURPHY: "λRecombination and Recombineering", ECOSAL PLUS, pages 1 - 70 *
MICHAEL E. PYNE ET AL.: "Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli", 《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》 *
MICHAEL E. PYNE ET AL.: "Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli", 《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》, 22 August 2015 (2015-08-22) *
STEVEN LIN ET AL.: "Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery", 《CELL BIOLOGY GENES AND CHROMOSOMES》 *
STEVEN LIN ET AL.: "Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery", 《CELL BIOLOGY GENES AND CHROMOSOMES》, 15 December 2014 (2014-12-15) *
XUN LIANG ET AL.: "A CRISPR/Cas9 and Cre/Lox systembased express vaccine development strategy against re-emerging Pseudorabies virus", 《SCIENTIFIC REPORTS》 *
XUN LIANG ET AL.: "A CRISPR/Cas9 and Cre/Lox systembased express vaccine development strategy against re-emerging Pseudorabies virus", 《SCIENTIFIC REPORTS》, 18 January 2016 (2016-01-18) *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113891938A (zh) * 2019-05-29 2022-01-04 孟山都技术公司 使用增强的靶向编辑技术的定向基因组工程
CN112979821A (zh) * 2019-12-18 2021-06-18 华东师范大学 一种提高基因编辑效率的融合蛋白及其应用
CN111518220A (zh) * 2020-05-14 2020-08-11 重庆英茂盛业生物科技有限公司 一种融合蛋白及其设计方法
CN111909961A (zh) * 2020-08-21 2020-11-10 华侨大学 一种CRISPR/Cas介导的ATL细胞基因编辑载体及其应用

Also Published As

Publication number Publication date
JP2020511931A (ja) 2020-04-23
CL2018003649A1 (es) 2019-07-05
US11708588B2 (en) 2023-07-25
WO2018229521A1 (en) 2018-12-20
WO2018229521A8 (en) 2019-02-28
US20230416788A1 (en) 2023-12-28
US20220010336A1 (en) 2022-01-13
EP3472329A1 (en) 2019-04-24
CA3028074A1 (en) 2018-12-20

Similar Documents

Publication Publication Date Title
JP7090775B2 (ja) 遺伝子産物の発現を変更するためのＣＲＩＳＰＲ－Ｃａｓ系および方法
JP7198328B2 (ja) 配列操作のための系、方法および最適化ガイド組成物のエンジニアリング
JP7383062B2 (ja) 配列操作のための最適化されたＣＲＩＳＰＲ－Ｃａｓ二重ニッカーゼ系、方法および組成物
JP6395765B2 (ja) 配列操作のための改善された系、方法および酵素組成物のエンジニアリングおよび最適化
DK2921557T3 (en) Design of systems, methods and optimized sequence manipulation guide compositions
CN109790551A (zh) 改进的基因编辑
HK40005733B (en) Engineered nuceic acid-targeting nucleic acids
HK1216759B (zh) 用於序列操纵的系统、方法和优化的指导组合物的工程化

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20190521

WD01 Invention patent application deemed withdrawn after publication