CN109781682A - The measuring method of Dissolved Organic Matter in a kind of breeding wastewater duckweed processing system - Google Patents
The measuring method of Dissolved Organic Matter in a kind of breeding wastewater duckweed processing system Download PDFInfo
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Abstract
The invention discloses a kind of measuring method of Dissolved Organic Matter in breeding wastewater duckweed processing system, the measuring method the following steps are included: detection breeding wastewater duckweed processing system in heavy metal concentration, obtain breeding wastewater in heavy metal concentration；For the heavy metal concentration in the breeding wastewater of acquisition, molecular size range and type that preset heavy metal concentration/fluorescent components tables of data obtains Dissolved Organic Matter in breeding wastewater duckweed processing system are inquired, wherein heavy metal concentration/fluorescent components tables of data includes heavy metal concentration, Dissolved Organic Matter molecular size range and their corresponding mapping relations and heavy metal concentration, Dissolved Organic Matter type and their corresponding mapping relations.Measuring method of the present invention has many advantages, such as quick, stable, reliable, efficient, the component of DOM in duckweed processing system can not only be identified, relationship between clear DOM each component and heavy metal concentration simultaneously, has a good application prospect in the actual treatment of breeding wastewater.
Description
Technical field
The invention belongs to technical field of biological environmental protection, it is related to Dissolved Organic Matter in a kind of breeding wastewater duckweed processing system
Measuring method.
Background technique
In recent years, copper, zinc are applied to livestock and poultry breeding industry as feed addictive extensively, since the anaerobism of breeding wastewater disappears
Change is difficult to handle the heavy metals such as copper, zinc, causes still to contain high concentration copper, zinc in biogas slurry.The compound dirt of copper, zinc, ammonia nitrogen and total phosphorus
Dye proposes stern challenge to traditional processing method, including microbiological treatment and phytoremediation pig raising biogas slurry.
As a kind of phytoremediation technology, duckweed system is widely used in the processing of pig raising biogas slurry.Duckweed is divided because it has
The notable features such as cloth is extensively, anti-pollution, the speed of growth are fast, easily harvest, are the important plants of the phytoremediation technology of water pollution
One of monoid.But due to the presence of heavy metal, the growth of duckweed and microorganism will receive influence, and therefore, duckweed system is answered
With being greatly limited.
In order to mitigate heavy metal copper zinc to the pressure coercion of duckweed, duckweed can be with by the antioxidant system of itself
Generate a variety of antioxidants.Dissolved Organic Matter (DOM) is exactly one such antioxidant, it belongs to water plant and rhizosphere
The heterogeneous mixture that microbial cell Metabolism Excretion or self-dissolving generate.This mix ingredients is complicated, property multiplicity, and be easy with
Complexing occurs for metal, affects heavy metal in the Transport And Transformation of aquatic system.Although there is many researchs to report DOM in water
The effect of processing system, but the ingredient of the DOM of duckweed system release and property have not been reported.
Under the irradiation of the exciting light of specific wavelength, the DOM in water can issue the transmitting light of specific wavelength, and it is different at
The DOM divided has different position peaks, therefore three-dimensional fluorescence spectrum (EEMs) can be used to characterize the composition of DOM in water.Usually
" peak-seeking method " is used to carry out qualitative analysis to fluorescence peak.But due to the ingredient of DOM complexity, overlapped fluorescence peak, merely
Peak-seeking method can not be to carrying out going deep into parsing, to increase the difficulty of fluorophor qualitative analysis.Parallel factor method is as one
The algorithm of kind fluorescence data processing, combines with fluorescence excitation-emission matrix (EEM) spectrum, has been used as studying DOM extensively
A kind of effective ways of composition, it, which can be reduced, does not assimilate interference overlapped between the conjunction object of fluorogen, so that it is determined that DOM
In single fluorescent components.As emerging water pollutant analytical technology, three-dimensional fluorescence spectrum combination parallel factor method has
High sensitivity does not destroy the advantages that sample structure, is widely used in DOM qualitative and quantitative analysis in the water body of rivers and lakes, detects, traces back
The fields such as source.However, environmental factor such as heavy metal and the interaction between DOM fluorescent components are unclear.Accordingly it is determined that
Qualitative, quantitative relationship in DOM between each fluorescent components and environmental factor is improved to Transport And Transformations such as environmental factor such as heavy metals
Understanding is one of current critical issue urgently to be solved.Therefore, find it is a kind of quickly, stablize, be reliable, efficient measurement cultivation it is useless
The method of Dissolved Organic Matter in water duckweed processing system, be very it is necessary to.
Summary of the invention
The technical problem to be solved by the present invention is to overcome deficiency in the prior art, provide it is a kind of quickly, stablize, be reliable,
The measuring method of Dissolved Organic Matter in efficient breeding wastewater duckweed processing system.
In order to solve the above technical problems, the technical solution adopted by the present invention is that:
The measuring method of Dissolved Organic Matter in a kind of breeding wastewater duckweed processing system, comprising the following steps:
Heavy metal concentration in S1, detection breeding wastewater duckweed processing system, obtains the heavy metal concentration in breeding wastewater；
S2, for the heavy metal concentration in the breeding wastewater of acquisition, inquire preset heavy metal concentration/fluorescent components data
Table obtains the molecular size range and type of Dissolved Organic Matter in breeding wastewater duckweed processing system；The heavy metal concentration/glimmering
Light component tables of data includes heavy metal concentration, Dissolved Organic Matter molecular size range and their corresponding mapping relations and a huge sum of money
Belong to concentration, Dissolved Organic Matter type and their corresponding mapping relations.
Above-mentioned measuring method, it is further improved, in the step S2, the heavy metal concentration/fluorescent components data
The building of table, comprising the following steps:
(1) duckweed plantation is cultivated in duckweed processing system, is adopted in the breeding wastewater of different heavy metal concentrations
Collect the breeding wastewater water sample of corresponding different heavy metal concentrations under the conditions of different incubation times；
(2) three-dimensional fluorescence spectrum measurement is carried out to water sample obtained in step (1), obtains the fluorescence spectrum number of different water samples
According to；
(3) parallel factor analysis is carried out to the fluorescence data of difference water sample obtained in step (2), obtained different heavy
The corresponding effective fluorescent components data of metal concentration and fluorescent components figure；
(4) effective fluorescent components data corresponding to difference heavy metal concentration obtained in step (3) carry out principal component point
Heavy metal concentration, Dissolved Organic Matter molecular size range and their corresponding mapping relations and heavy metal concentration, molten are established in analysis
Solution property organic molecule amount size and their corresponding mapping relations, obtain heavy metal concentration/fluorescent components tables of data.
Above-mentioned measuring method, further improved, in the step (1): the plantation duty ratio of the duckweed is by fresh
Duckweed 1.6g~3g is planted in every liter of breeding wastewater of restatement；Corresponding heavy metal in the breeding wastewater of the difference heavy metal concentration
Concentration is respectively 0mg/L, 0.1mg/L, 0.5mg/L, 1.0mg/L, 2.0mg/L, 5.0mg/L；The breeding wastewater is pig raising natural pond
Liquid；In the breeding wastewater COD be 100mg/L~200mg/L, ammonia nitrogen be 60mg/L~90mg/L, total phosphorus be 10mg/L~
15mg/L；The duckweed is dilute floating pulse duckweed；The pH value that breeding wastewater is controlled in the incubation is 6.1~6.5；The water sample
The acquisition interval time be 4h~8h.
Above-mentioned measuring method, it is further improved, in the step (2): further including before measurement three-dimensional fluorescence spectrum
Following steps: the filter membrane that the water sample via hole diameter that step (1) obtains is 0.45 μm is filtered；Resulting clear liquid dilution will be filtered
To TOC concentration≤10.9mg/L；The location parameter of the three-dimensional fluorescence spectrum are as follows: temperature is 25 DEG C~30 DEG C, excitation wavelength
Range is 200~500nm, is scanned with the increment of 10nm, and the range of launch wavelength is 200nm~600nm, with the increasing of 2nm
Amount is scanned；The slit width of the excitation wavelength and launch wavelength is 10nm；The measurement of the three-dimensional fluorescence spectrum
Fluorescent scanning speed 12000nm/min in journey, single sweep operation time are less than 1min.
Above-mentioned measuring method, further improved, in the step (3): the parallel factor analysis includes scattering peak
Removal, Raman units correction, abnormality value removing, residual analysis, random assignment analysis and model verifying；The fluorescent components figure
Excitation wavelength is removed in drawing process and launch wavelength is lower than the data of 250nm；The fluorescent components figure by with it is known glimmering
Light constitutional diagram compares, and determines the composition of Dissolved Organic Matter in breeding wastewater duckweed processing system.
Above-mentioned measuring method, it is further improved, in the step S2, inquire default heavy metal concentration/fluorescent components
Heavy metal concentration, Dissolved Organic Matter molecular size range and their corresponding mapping relations and heavy metal in tables of data is dense
Degree, Dissolved Organic Matter type and their corresponding mapping relations obtain dissolubility in breeding wastewater duckweed processing system respectively and have
The molecular size range and type of machine matter.
Above-mentioned measuring method, it is further improved, the heavy metal concentration, Dissolved Organic Matter molecular size range and it
In corresponding mapping relations, heavy metal concentration is divided into 3 sections, be respectively as follows: M1≤0.5mg/L, 0.5mg/L < M2≤
1mg/L, 1mg/L < M3；
When the heavy metal concentration section is M1, indicate to produce in breeding wastewater duckweed processing system without Dissolved Organic Matter
It is raw；
When the heavy metal concentration section is M2, the Dissolved Organic Matter generated in breeding wastewater duckweed processing system is indicated
Based on the Dissolved Organic Matter of molecular weight < 10000Da；
When the heavy metal concentration section is M3, the Dissolved Organic Matter generated in breeding wastewater duckweed processing system is indicated
Based on molecular weight >=10000Da Dissolved Organic Matter.
Above-mentioned measuring method, further improved, the heavy metal concentration, Dissolved Organic Matter type and their correspondences
Mapping relations in, heavy metal concentration is divided into 2 sections, is respectively as follows: C1≤0.1mg/L, 0.1mg/L < C2；
When the heavy metal concentration section is C1, indicates that Dissolved Organic Matter type is mainly microbial product, show to support
The trophic level for growing waste water duckweed processing system is normal, conducive to the growth of plant and microorganism；
When the heavy metal concentration section is C2, indicates that Dissolved Organic Matter type is mainly acidic materials, show to cultivate
The trophic level of waste water duckweed processing system is abnormal, is unfavorable for the growth of plant and microorganism.
Above-mentioned measuring method, it is further improved, the microbial product be microbial metabolism generate humic acid and/
Or reduced coenzyme；The acidic materials are the humic acid that tyrosine and/or plant metabolism generate.
Above-mentioned measuring method, further improved, the heavy metal is Cu or Zn.
Compared with the prior art, the advantages of the present invention are as follows:
(1) the present invention provides a kind of measuring method of Dissolved Organic Matter in breeding wastewater duckweed processing system, pass through
Heavy metal concentration in detection acquisition breeding wastewater, and the preset heavy metal concentration of the inquiry of the heavy metal concentration by obtaining/glimmering
Light component tables of data is the molecular size range and type of Dissolved Organic Matter in desirable breeding wastewater duckweed processing system, thus real
Now to the measurement of Dissolved Organic Matter in breeding wastewater duckweed processing system, have many advantages, such as it is quick, stable, reliable, efficient, and
Measuring method of the present invention has many advantages, such as simple process, easy to operate, low in cost, can not only quick, sensitive, accurately reflect
The component of DOM in other duckweed processing system, while in clear duckweed processing system in DOM between each component and heavy metal concentration
Relationship has a good application prospect in the actual treatment of breeding wastewater.
(2) in measuring method of the present invention, using Principal Component Analysis to the number of parallel factor method fluorescent components obtained
According to being further processed, have quickly, stablize, reliable efficient processing feature, can in qualitative determination DOM each fluorescent components with
Heavy metal interaction relationship had both been able to achieve " the mathematics separation " of DOM fluorescence information in duckweed system, and can also specify duckweed system
The qualitative relationships of middle metal concentration and DOM fluorescent components can provide advantageous information for the design and management of duckweed system.
Detailed description of the invention
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, the technical scheme in the embodiment of the invention is clearly and completely described.
Fig. 1 is Cu concentration/fluorescent components tables of data building flow chart in the embodiment of the present invention 1.
Fig. 2 is the fluorescent components figure of Dissolved Organic Matter in the breeding wastewater duckweed processing system of the embodiment of the present invention 1.
Fig. 3 is the relational graph in the embodiment of the present invention 1 between Cu concentration and Dissolved Organic Matter each component.
Specific embodiment
Below in conjunction with Figure of description and specific preferred embodiment, the invention will be further described, but not therefore and
It limits the scope of the invention.
Embodiment 1:
The measuring method of Dissolved Organic Matter in a kind of breeding wastewater duckweed processing system, comprising the following steps:
Cu concentration in S1, detection breeding wastewater duckweed processing system, obtains the Cu concentration in breeding wastewater；
S2, for the Cu concentration in the breeding wastewater of acquisition, inquire preset Cu concentration/fluorescent components tables of data and obtain and support
The molecular size range and type of Dissolved Organic Matter in waste water duckweed processing system are grown, wherein Cu concentration/fluorescent components tables of data
Include Cu concentration, Dissolved Organic Matter molecular size range and their corresponding mapping relations and Cu concentration, Dissolved Organic Matter
Type and their corresponding mapping relations.
In the present embodiment, the Cu concentration preset in Cu concentration/fluorescent components tables of data, Dissolved Organic Matter molecular weight are inquired
Size and their corresponding mapping relations and Cu concentration, Dissolved Organic Matter type and their corresponding mapping relations obtain respectively
Take the molecular size range and type of Dissolved Organic Matter in breeding wastewater duckweed processing system.
Cu concentration, Dissolved Organic Matter molecular size range in the present embodiment, in default Cu concentration/fluorescent components tables of data
And in their corresponding mapping relations, Cu concentration is divided into 3 sections, is respectively as follows: M1≤0.5mg/L, 0.5mg/L < M2
≤ 1mg/L, 1mg/L < M3；When Cu concentration ranges are M1, indicate in breeding wastewater duckweed processing system without Dissolved Organic Matter
It generates；When Cu concentration ranges are M2, indicate the Dissolved Organic Matter generated in breeding wastewater duckweed processing system with molecular weight
Based on the Dissolved Organic Matter of < 10000Da；When heavy metal concentration section is M3, indicate in breeding wastewater duckweed processing system
The Dissolved Organic Matter of generation is based on molecular weight >=10000Da Dissolved Organic Matter.
In the present embodiment, preset Cu concentration/fluorescent components tables of data in Cu concentration, Dissolved Organic Matter type and they
In corresponding mapping relations, Cu concentration is divided into 2 sections, is respectively as follows: C1≤0.1mg/L, 0.1mg/L < C2；Cu concentration
When section is C1, indicates that Dissolved Organic Matter type is mainly microbial product, show the battalion of breeding wastewater duckweed processing system
It is normal to support level, conducive to the growth of plant and microorganism；When Cu concentration ranges are C2, indicate that Dissolved Organic Matter type is mainly
Acidic materials show that the trophic level of breeding wastewater duckweed processing system is abnormal, are unfavorable for the growth of plant and microorganism.This
In embodiment, microbial product is the humic acid and/or reduced coenzyme that microbial metabolism generates；Acidic materials be tyrosine and
/ or plant metabolism generate humic acid.
In the present embodiment, the building of Cu concentration/fluorescent components tables of data, flow chart is as shown in Figure 1, include following step
It is rapid:
(1) prepare 1L difference Cu concentration (gradient of Cu concentration be 0mg/L, 0.1mg/L, 0.5mg/L, 1.0mg/L,
2.0mg/L, 5.0mg/L) pig raising biogas slurry (in the pig raising biogas slurry, COD 150mg/L, ammonia nitrogen 82mg/L, total phosphorus 15mg/
L), duckweed is planted into the pig raising biogas slurry of different Cu concentration gradients respectively to be placed in breeding wastewater duckweed processing system, wherein
The duty ratio for planting duckweed is 3g duckweed fresh weight/1L pig raising biogas slurry.Adjust the initial pH for biogas slurry of respectively raising pigs in duckweed processing system
Value is 6.4, is cultivated in duckweed processing system.During the cultivation process, it is adjusted daily with the NaOH solution of 0.1mol/L floating
The pH value of duckweed processing system and to control pH value be 6.4, and (it was right under the conditions of different incubation times to acquire every 5 days acquisition water samples
The breeding wastewater water sample for the different Cu concentration answered), water sample is drawn with 10mL syringe when acquisition.
(2) water sample obtained in step (1) is crossed into the filter membrane that aperture is 0.45 μm, be filtered respectively, utilize TOC
Total dissolved organic carbon (DOC) of measurement filtering gained clear liquid, obtains sample DOC concentration on analyzer (Shimadzu, Japan), according to
Need the DOC concentration to less than 10.9mg/L with ultrapure water dilute sample；Using (Hitachi, Japan) on three-dimensional fluorescence analyzer
The three-dimensional fluorescence spectrum of sample after measurement dilution, obtains the fluorescence data of different water samples, and wherein fluorescence spectrometry is joined
Number are as follows: temperature is 25 DEG C~30 DEG C, and the range of excitation wavelength is 200~500nm, is scanned with the increment of 10nm, transmitted wave
Long range is 200nm~600nm, is scanned with the increment of 2nm.The slit width of excitation wavelength and launch wavelength is
10nm.Fluorescent scanning speed 12000nm/min, single sweep operation time are less than 1min.
(3) fluorescence data obtained in step (2) is converted into .CSV file, imports MatLab platform, utilized
DOMFluor program parses three-dimensional fluorescence spectrum using parallel factor analysis (PARAFAC), mainly goes including scattering peak
It removes, Raman units correction, abnormality value removing, residual analysis, random assignment analysis, model verifying, it is corresponding to obtain different Cu concentration
Effective fluorescent components data (including effective fluorescent components number, effective fluorescent components) and fluorescent components figure.Fluorescent components figure
Excitation wavelength and launch wavelength should all remove the data that wavelength is lower than 250nm in drafting, to reduce the noise as caused by low wavelength,
Improve accuracy.Fig. 2 is the fluorescent components of Dissolved Organic Matter in the breeding wastewater duckweed processing system of the embodiment of the present invention 1
Figure.As shown in Figure 2, in the present embodiment, finally obtaining the model being verified has 4, shows the DOM of duckweed processing system release
Be mainly made of 4 fluorescent components.By being compared with known fluorescent components figure, it is determined that the title of fluorescent components, i.e., originally
The composition of DOM in the duckweed processing system of embodiment are as follows: the humic acid (C1) of microbial metabolism generation, tyrosine (C2), reduction
The humic acid (C4) that type coenzyme (NADH) (C3), plant metabolism generate, as a result as shown in Figure 2.
(4) by the fluorescent components data obtained in step (3) (the corresponding effective fluorescent components number of as different Cu concentration
According to) PAST3.0 software is imported, the principal component analysis of fluorescence data is carried out, it is big to establish Cu concentration, Dissolved Organic Matter molecular weight
Small and their corresponding mapping relations and Cu concentration, Dissolved Organic Matter type and their corresponding mapping relations obtain weight
Relationship between metal concentration/fluorescent components tables of data, as Cu concentration and Dissolved Organic Matter each component, as a result as shown in Figure 3.
Fig. 3 is the relational graph in the embodiment of the present invention 1 between Cu concentration and Dissolved Organic Matter each component.In Fig. 3, PC1 be first it is main at
Point, for being expressed as the size of DOM molecular weight, when wherein PC1 is negative value, it is expressed as system and is generated without DOM, PC1 is small just
Value is expressed as in system based on the DOM of high molecular weight；PC1 be big positive value be expressed as in system be with the DOM of low molecular weight
It is main.PC2 is Second principal component, for indicating that the trophic level of system indicates the trophic level of system when value PC2 is positive value
Mainly microbial product, system trophism are normal；When PC2 is negative value, indicates that the trophic level of system is mainly acidic materials, be
System nutrition meta-acid, is unfavorable for the growth of plant and microorganism.
In the present embodiment, when Cu concentration is 1.0mg/L, show under the conditions of low concentration Cu, the processing of breeding wastewater duckweed
Indicate that the Dissolved Organic Matter generated in breeding wastewater duckweed processing system has with the dissolubility of molecular weight < 10000Da in system
Based on machine matter；When Cu concentration is 2.0-5.0mg/L, show under the conditions of high concentration Cu, in breeding wastewater duckweed processing system
The Dissolved Organic Matter of generation is based on molecular weight >=10000Da Dissolved Organic Matter.
In the present embodiment, when Cu concentration is 0.1mg/L, show under the conditions of low concentration Cu, the processing of breeding wastewater duckweed
Dissolved Organic Matter type is mainly microbial product (humic acid and reduced coenzyme that microbial metabolism generates), table in system
The trophic level of bright breeding wastewater duckweed processing system is normal, conducive to the growth of plant and microorganism；When Cu concentration is 2.0-
When 5.0mg/L, show under the conditions of high concentration Cu, Dissolved Organic Matter type is mainly in breeding wastewater duckweed processing system
Acidic materials (humic acid that tyrosine and plant metabolism generate), show the trophic level of breeding wastewater duckweed processing system not
Normally, it is unfavorable for the growth of plant and microorganism.
Above embodiments are only the preferred embodiment of the present invention, and protection scope of the present invention is not limited merely to above-mentioned reality
Apply example.All technical solutions belonged under thinking of the present invention all belong to the scope of protection of the present invention.It is noted that being led for this technology
For the those of ordinary skill in domain, improvements and modifications without departing from the principle of the present invention, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (10)
1. the measuring method of Dissolved Organic Matter in a kind of breeding wastewater duckweed processing system, which is characterized in that including following step
It is rapid:
Heavy metal concentration in S1, detection breeding wastewater duckweed processing system, obtains the heavy metal concentration in breeding wastewater；
S2, for the heavy metal concentration in the breeding wastewater of acquisition, inquire preset heavy metal concentration/fluorescent components tables of data and obtain
Take the molecular size range and type of Dissolved Organic Matter in breeding wastewater duckweed processing system；The heavy metal concentration/fluorescence group
Divided data table includes that heavy metal concentration, Dissolved Organic Matter molecular size range and their corresponding mapping relations and heavy metal are dense
Degree, Dissolved Organic Matter type and their corresponding mapping relations.
2. measuring method according to claim 1, which is characterized in that in the step S2, the heavy metal concentration/fluorescence
The building of component tables of data, comprising the following steps:
(1) duckweed plantation is cultivated in duckweed processing system in the breeding wastewater of different heavy metal concentrations, acquisition is not
The breeding wastewater water sample of corresponding difference heavy metal concentration under the conditions of incubation time；
(2) three-dimensional fluorescence spectrum measurement is carried out to water sample obtained in step (1), obtains the fluorescence data of different water samples；
(3) parallel factor analysis is carried out to the fluorescence data of difference water sample obtained in step (2), obtains different heavy metals
The corresponding effective fluorescent components data of concentration and fluorescent components figure；
(4) effective fluorescent components data corresponding to difference heavy metal concentration obtained in step (3) carry out principal component analysis, build
Vertical heavy metal concentration, Dissolved Organic Matter molecular size range and their corresponding mapping relations and heavy metal concentration, dissolubility
Organic molecule amount size and their corresponding mapping relations, obtain heavy metal concentration/fluorescent components tables of data.
3. measuring method according to claim 2, which is characterized in that in the step (1): the plantation load of the duckweed
Than in every liter based on fresh weight breeding wastewater plant duckweed 1.6g~3g；It is corresponding in the breeding wastewater of the difference heavy metal concentration
Heavy metal concentration be respectively 0mg/L, 0.1mg/L, 0.5mg/L, 1.0mg/L, 2.0mg/L, 5.0mg/L；The breeding wastewater
For biogas slurry of raising pigs；COD is 100mg/L~200mg/L in the breeding wastewater, and ammonia nitrogen is 60mg/L~90mg/L, and total phosphorus is
10mg/L~15mg/L；The duckweed is dilute floating pulse duckweed；The pH value that breeding wastewater is controlled in the incubation is 6.1~6.5；
The acquisition interval time of the water sample is 4h~8h.
4. measuring method according to claim 2, which is characterized in that in the step (2): measurement three-dimensional fluorescence spectrum it
It is preceding further comprising the steps of: the filter membrane that the water sample via hole diameter that step (1) obtains is 0.45 μm is filtered；It will filter resulting
Clear liquid is diluted to TOC concentration≤10.9mg/L；The location parameter of the three-dimensional fluorescence spectrum are as follows: temperature is 25 DEG C~30 DEG C, is swashed
The range for sending out wavelength is 200~500nm, is scanned with the increment of 10nm, and the range of launch wavelength is 200nm~600nm, with
The increment of 2nm is scanned；The slit width of the excitation wavelength and launch wavelength is 10nm；The three-dimensional fluorescence spectrum
Fluorescent scanning speed 12000nm/min in continuous mode, single sweep operation time are less than 1min.
5. measuring method according to claim 2, which is characterized in that in the step (3): the parallel factor analysis packet
Include scattering peak removal, Raman units correction, abnormality value removing, residual analysis, random assignment analysis and model verifying；The fluorescence
Excitation wavelength is removed in the drawing process of constitutional diagram and launch wavelength is lower than the data of 250nm；The fluorescent components figure by with
Known fluorescent components figure compares, and determines the composition of Dissolved Organic Matter in breeding wastewater duckweed processing system.
6. measuring method according to claim 1, which is characterized in that in the step S2, the default heavy metal concentration of inquiry/
Heavy metal concentration, Dissolved Organic Matter molecular size range and their corresponding mapping relations in fluorescent components tables of data and again
Metal concentration, Dissolved Organic Matter type and their corresponding mapping relations obtain molten in breeding wastewater duckweed processing system respectively
The molecular size range and type of solution property organic matter.
7. measuring method according to claim 6, which is characterized in that the heavy metal concentration, Dissolved Organic Matter molecule
Measure in size and their corresponding mapping relations, heavy metal concentration be divided into 3 sections, be respectively as follows: M1≤0.5mg/L,
0.5mg/L < M2≤1mg/L, 1mg/L < M3；
When the heavy metal concentration section is M1, indicate to generate in breeding wastewater duckweed processing system without Dissolved Organic Matter；
When the heavy metal concentration section is M2, the Dissolved Organic Matter generated in breeding wastewater duckweed processing system is indicated to divide
Based on the Dissolved Organic Matter of son amount < 10000Da；
When the heavy metal concentration section is M3, the Dissolved Organic Matter generated in breeding wastewater duckweed processing system is indicated to divide
Based on the Dissolved Organic Matter of sub- amount >=10000Da.
8. measuring method according to claim 6, which is characterized in that the heavy metal concentration, Dissolved Organic Matter type
And in their corresponding mapping relations, heavy metal concentration is divided into 2 sections, is respectively as follows: C1≤0.1mg/L, 0.1mg/L <
C2；
It when the heavy metal concentration section is C1, indicates that Dissolved Organic Matter type is mainly microbial product, shows that cultivation is useless
The trophic level of water duckweed processing system is normal, conducive to the growth of plant and microorganism；
When the heavy metal concentration section is C2, indicates that Dissolved Organic Matter type is mainly acidic materials, show breeding wastewater
The trophic level of duckweed processing system is abnormal, is unfavorable for the growth of plant and microorganism.
9. measuring method according to claim 8, which is characterized in that the microbial product is what microbial metabolism generated
Humic acid and/or reduced coenzyme；The acidic materials are the humic acid that tyrosine and/or plant metabolism generate.
10. measuring method described according to claim 1~any one of 9, which is characterized in that the heavy metal is Cu or Zn.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110835162A (en) * | 2019-10-28 | 2020-02-25 | 东珠生态环保股份有限公司 | Method for restoring heavy metal polluted water body by utilizing ecological cycle of aquatic plant duckweed |
| CN113933256A (en) * | 2021-09-22 | 2022-01-14 | 武汉纺织大学 | System and method for detecting odor adsorption and release performance of textiles |
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