CN109777443A - A kind of soil heavy metal pollution remediation agent - Google Patents
A kind of soil heavy metal pollution remediation agent Download PDFInfo
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- CN109777443A CN109777443A CN201910204345.1A CN201910204345A CN109777443A CN 109777443 A CN109777443 A CN 109777443A CN 201910204345 A CN201910204345 A CN 201910204345A CN 109777443 A CN109777443 A CN 109777443A
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- Prior art keywords
- soil
- heavy metal
- saccharomycete
- metal pollution
- nanoscale iron
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- 239000002689 soil Substances 0.000 title claims abstract description 91
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 40
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 31
- 238000005067 remediation Methods 0.000 title abstract 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 164
- 229910052742 iron Inorganic materials 0.000 claims abstract description 84
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims abstract description 58
- 238000002360 preparation method Methods 0.000 claims abstract description 50
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 49
- 241000208125 Nicotiana Species 0.000 claims abstract description 40
- 229910052793 cadmium Inorganic materials 0.000 claims abstract description 32
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 claims abstract description 32
- 238000000034 method Methods 0.000 claims abstract description 16
- 239000002699 waste material Substances 0.000 claims abstract description 11
- 229940055329 tobacco leaf extract Drugs 0.000 claims abstract description 9
- 238000006243 chemical reaction Methods 0.000 claims abstract description 4
- 241000235342 Saccharomycetes Species 0.000 claims description 69
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 47
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 34
- 239000000284 extract Substances 0.000 claims description 29
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 25
- 238000009418 renovation Methods 0.000 claims description 24
- 238000010521 absorption reaction Methods 0.000 claims description 22
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 15
- 229910052799 carbon Inorganic materials 0.000 claims description 13
- 239000000243 solution Substances 0.000 claims description 13
- 238000003756 stirring Methods 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 8
- 238000002137 ultrasound extraction Methods 0.000 claims description 8
- 238000005406 washing Methods 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 230000010355 oscillation Effects 0.000 claims description 6
- 238000000926 separation method Methods 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 5
- UORVGPXVDQYIDP-UHFFFAOYSA-N borane Chemical compound B UORVGPXVDQYIDP-UHFFFAOYSA-N 0.000 claims description 5
- 229910010277 boron hydride Inorganic materials 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 238000007796 conventional method Methods 0.000 claims description 4
- 238000000605 extraction Methods 0.000 claims description 4
- 238000004108 freeze drying Methods 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 4
- 238000004821 distillation Methods 0.000 claims description 3
- 238000000227 grinding Methods 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 230000002045 lasting effect Effects 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 238000005119 centrifugation Methods 0.000 claims description 2
- 238000002156 mixing Methods 0.000 abstract description 4
- 231100000331 toxic Toxicity 0.000 abstract description 3
- 230000002588 toxic effect Effects 0.000 abstract description 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 2
- 238000011065 in-situ storage Methods 0.000 abstract description 2
- 239000004016 soil organic matter Substances 0.000 abstract 1
- 238000012545 processing Methods 0.000 description 20
- 244000061176 Nicotiana tabacum Species 0.000 description 18
- 240000007594 Oryza sativa Species 0.000 description 15
- 235000007164 Oryza sativa Nutrition 0.000 description 15
- 235000009566 rice Nutrition 0.000 description 15
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 11
- 239000002253 acid Substances 0.000 description 9
- 235000019441 ethanol Nutrition 0.000 description 8
- 238000002161 passivation Methods 0.000 description 6
- 230000009467 reduction Effects 0.000 description 4
- 230000008439 repair process Effects 0.000 description 4
- 238000012136 culture method Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000010903 husk Substances 0.000 description 3
- 244000269722 Thea sinensis Species 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 238000007605 air drying Methods 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 239000006286 aqueous extract Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000003610 charcoal Substances 0.000 description 2
- 239000003638 chemical reducing agent Substances 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 229910052603 melanterite Inorganic materials 0.000 description 2
- 238000003900 soil pollution Methods 0.000 description 2
- 239000002344 surface layer Substances 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 241000282693 Cercopithecidae Species 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- 244000004281 Eucalyptus maculata Species 0.000 description 1
- 240000006053 Garcinia mangostana Species 0.000 description 1
- 235000017048 Garcinia mangostana Nutrition 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- JOPOVCBBYLSVDA-UHFFFAOYSA-N chromium(6+) Chemical compound [Cr+6] JOPOVCBBYLSVDA-UHFFFAOYSA-N 0.000 description 1
- 235000020971 citrus fruits Nutrition 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- IYRDVAUFQZOLSB-UHFFFAOYSA-N copper iron Chemical compound [Fe].[Cu] IYRDVAUFQZOLSB-UHFFFAOYSA-N 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000009569 green tea Nutrition 0.000 description 1
- 229940094952 green tea extract Drugs 0.000 description 1
- 235000020688 green tea extract Nutrition 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- UGKDIUIOSMUOAW-UHFFFAOYSA-N iron nickel Chemical compound [Fe].[Ni] UGKDIUIOSMUOAW-UHFFFAOYSA-N 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000001771 mentha piperita Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000012279 sodium borohydride Substances 0.000 description 1
- 229910000033 sodium borohydride Inorganic materials 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 235000013616 tea Nutrition 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
Landscapes
- Processing Of Solid Wastes (AREA)
Abstract
本发明提供一种土壤重金属污染修复剂，包括酵母菌载体和其负载的纳米铁，其中，纳米铁是以废弃烟叶提取物代替硼氢化物制备的酵母菌载纳米铁。将其与土壤混匀，可促进土壤中的铅和镉转化为残渣态，达到降低土壤中铅和镉的生物有效性的目的。该酵母菌载纳米铁制备不需要任何有毒的试剂，在室温常压下使用常规仪器便能完成。不仅能实现废弃烟叶的资源化利用，降低纳米铁的制备成本，环境友好，而且能在修复土壤重金属污染的同时，增加土壤有机质含量，提高农作物的产质量。可广泛用于铅、镉污染土壤的原位修复，属于重金属污染修复技术领域。The invention provides a soil heavy metal pollution remediation agent, comprising a yeast carrier and nano-iron carried thereon, wherein the nano-iron is a yeast-carried nano-iron prepared by replacing borohydride with waste tobacco leaf extract. Mixing it with the soil can promote the conversion of lead and cadmium in the soil into a residual state, and achieve the purpose of reducing the biological availability of lead and cadmium in the soil. The preparation of the yeast-loaded nano-iron does not require any toxic reagents, and can be completed using conventional instruments at room temperature and normal pressure. The method can not only realize the resource utilization of waste tobacco leaves, reduce the preparation cost of nano-iron, and be environmentally friendly, but also can increase the content of soil organic matter and improve the yield quality of crops while repairing heavy metal pollution in the soil. The invention can be widely used for in-situ restoration of lead and cadmium polluted soil, and belongs to the technical field of heavy metal pollution restoration.
Description
Technical field
The present invention provides a kind of heavy metal pollution of soil renovation agent, the Nanoscale Iron including yeast vector and its load,
In, Nanoscale Iron is that the saccharomycete of boron hydride preparation is replaced to carry Nanoscale Iron with waste tobacco leaf extract, for lead-contaminated soil and
The reparation of cadmium pollution soil.Belong to heavy metal pollution of soil recovery technique field.
Background technique
In recent years, crop heavy metal over-standard phenomenon happens occasionally, and the heavy metal pollution of soil is one of the main reasons for this.
Heavy metal pollution renovation agent is applied, by changing the occurrence patterns of heavy metal, reducing its migration and biology in the soil has
Effect property is one of the effective way of repairing heavy metal in soil pollution.
The high reproducibility of nano zero valence iron and excellent absorption property become a kind of soil huge sum of money for having much application potential
Belong to pollution amelioration agent.Currently, the preparation of Nanoscale Iron is mostly using sodium borohydride or potassium borohydride as reducing agent, not only toxicity is big, and
And plus polymer or surfactant is needed to make the coating agent or stabilizer of Nanoscale Iron, there are certain environmental risks, and with
The plant extracts such as tealeaves, eucalyptus leaves, dried peppermint leaf, sorghum bran, orange peel and citrus are that prepare Nanoscale Iron not only at low cost for reducing agent
It is honest and clean and environmentally protective.Patent CN201710245090.4 disclose it is a kind of with 80 DEG C of water extract green-tea extract for reduction
The method that agent, dispersing agent and screening agent prepare nanometer iron powder, for pitch it is anti-aging and reduction road surface heavy metal ion；Patent
CN201610274411.9 discloses a kind of utilization tea free aqueous extract synthesis Nanoscale Iron removal p, the method for p '-DDT；Patent
CN201510824709.8 and CN201510824685.6 individually discloses a kind of utilization green tea aqueous extract synthesis nano zero-valence
Iron nickel bimetal and nano zero-valence iron-copper bi-metal material and its for the hexavalent chromium polluted side in rehabilitating soil and underground water
Method, both bimetallic materials require addition surfactant and are modified.In addition, patent CN201410112823.3 and
CN201310141406.7 then individually discloses a kind of hot water extracting solution green syt that leaf is liked using mangosteen peel and monkey
The method of Nanoscale Iron.
Saccharomycete has porous network structure and amino abundant, hydroxyl, carboxyl and phosphate group, is a kind of natural life
Object adsorbent.The Nanoscale Iron that waste tobacco leaf extract restores ferrous salt or molysite preparation is loaded on saccharomycete, yeast is made
Bacterium carries Nanoscale Iron, not only can be reduced the reunion between nano iron particles, but also can play the suction-operated and nanometer of saccharomycete simultaneously
The reduction of iron improves the utilization rate of Nanoscale Iron.The prior art yeast that disclosure is prepared not yet with waste tobacco leaf extract
Bacterium carries the method that Nanoscale Iron is used for repairing heavy metal in soil pollution.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of heavy metal pollution of soil renovation agent, including yeast vector and
Its Nanoscale Iron loaded, the Nanoscale Iron are to replace boron hydride with waste tobacco leaf extract, the ferrous salt that saccharomycete is adsorbed
Or molysite in-situ reducing, obtained saccharomycete carry Nanoscale Iron.By adjusting the mass ratio of yeast vector and ferrous salt or molysite,
And/or the dosage by adjusting leaf tobacco extract, and/or by adjusting reaction time, and/or by adjusting stirring or oscillation speed
It spends to control the reactivity of Nanoscale Iron.
Therefore, the first purpose of the invention is to provide a kind of heavy metal pollution of soil renovation agents, including yeast vector
It is that boron hydride is replaced with waste tobacco leaf extract with the Nanoscale Iron of its load, the ferrous salt of saccharomycete absorption or molysite is former
Position reduction gained.
Heavy metal pollution of soil reparation is prepared using waste tobacco leaf extract a second object of the present invention is to provide a kind of
The method of agent, comprising the following steps:
(1) tobacco leaf is handled: it after the tobacco natural for having washed off impurity is dried, dried at 50 DEG C, crushed 200 meshes,
Sealing is stand-by；
(2) prepared by leaf tobacco extract: by tobacco leaf powder obtained in above-mentioned steps (1) and Extraction solvent according to quality volume
Than for 1:5~1:40 (unit gmL-1), it is added in 250mL conical flask, 10~40min of ultrasonic extraction or shaken at room temperature mention
1~8h is taken, after centrifuge separation, clear liquid Rotary Evaporators are concentrated into 10mL at 38 DEG C or less；
(3) prepared by yeast vector: carrying out saccharomycete Liquid Culture according to a conventional method, resulting saccharomycete is centrifuged, is washed
After washing, it is freeze-dried to obtain yeast vector；
(4) ferrous salt or molysite aqueous solution yeast vector absorption ferrous salt or molysite: are added to what step (3) obtained
In yeast vector, in 50~200rmin-10.5~2h of lower stirring or oscillation obtains being adsorbed with ferrous salt after centrifugation, washing
Or the saccharomycete of molysite；
(5) Nanoscale Iron prepare: it is lasting stirring or oscillation under conditions of, toward step (4) obtain be adsorbed with ferrous salt or
The leaf tobacco extract that step (2) obtain is added in the saccharomycete of molysite, continues stirring or oscillating reactions 0.5h~8h, then consolidated
Liquid separation, solid carry Nanoscale Iron after freeze-drying grinding with second distillation water washing 3 times up to saccharomycete.
Preferably, the saccharomycete is Saccharomyces cerevisiae or saccharomyces cerevisiae.
Preferably, tobacco leaf powder described in step (2) is 1.0g, and Extraction solvent is 25mL 0~80% (volume ratio)
Ethanol solution.
Preferably, yeast vector quality described in step (4) is 0.02~1.0g, the ferrous salt or molysite with
The mass ratio of yeast vector is 1:2~30.
Preferably, step (5) is while stirring or oscillation with 0.5~0.8mLmin-1Gas supply speed toward described molten
Lead to N in liquid2, the separation of solid and liquid is in 3000~5000rmin-15~10min is centrifuged under revolving speed.
Third object of the present invention is to provide carry out heavy-metal contaminated soil using the heavy metal pollution of soil renovation agent
The method of reparation.
The restorative procedure of heavy-metal contaminated soil is as follows: applying heavy metal pollution of soil reparation into heavy-metal contaminated soil
Agent, after mixing well, routinely earth culture method is managed.Timing sampling, by BCR method (Tanta, 2006,70:973-978)
Measure the content of various forms heavy metal in soil.
In a specific embodiment, the soil preferably repaired is lead-contaminated soil.
In another specific embodiment, the soil preferably repaired is cadmium pollution soil.
Technical effect
(1) biologically effective of lead and cadmium in soil can be effectively reduced in heavy metal pollution of soil renovation agent prepared by the present invention
Property, promote lead and cadmium in soil to be converted into inactive residual form.Nanoscale Iron load significantly improves saccharomycete to soil weight
The passivation ability of metal: saccharomycete carries ferment of the ability than unsupported Nanoscale Iron that Nanoscale Iron converts the lead in soil to residual form
Female bacterium is high by 24.9%, and the ability that the cadmium in soil is converted residual form by it is higher than the saccharomycete of unsupported Nanoscale Iron by 12.4
Times.
(2) saccharomycete carries the rice husk carbon-supported nanometer that Nanoscale Iron is above equivalent to the passivation ability of lead and cadmium in soil
Iron.The ability that the lead in soil is converted residual form by saccharomycete load Nanoscale Iron is higher by 15.3% than rice hull carbon load Nanoscale Iron, will be native
Cadmium is converted into the ability of residual form than rice hull carbon to carry Nanoscale Iron strong by 155.8% in earth.
(3) with waste tobacco leaf extract replace toxic boron hydride prepare saccharomycete carry Nanoscale Iron, do not need using appoint
What toxic reagent, just can be completed using conventional instrument at room temperature, at a normal, not only be able to achieve the resource utilization of waste tobacco leaf,
The preparation cost of Nanoscale Iron is reduced, it is environmental-friendly, and the soil organism can be increased while repairing heavy metal in soil pollutes
Content improves the yield and quality of crops.
Detailed description of the invention
Fig. 1 is that heavy metal pollution of soil renovation agent handles the influence to form lead content each in lead-contaminated soil in 3 months.
Fig. 2 is that heavy metal pollution of soil renovation agent handles the influence to form cadmium content each in cadmium pollution soil in 2 months.
Specific embodiment
In the following, the present invention will be further detailed with embodiment, but its any for being not limited to these examples
A or similar example.
Embodiment 1:
(1) tobacco leaf is handled: after the tobacco natural for having washed off impurity is dried, being dried at 50 DEG C, be crushed 200 meshes；
(2) prepared by leaf tobacco extract: tobacco leaf powder obtained in 1.0g above-mentioned steps (1) is weighed in 250mL conical flask,
Be added 25mL50% ethanol solution (volume ratio) ultrasonic extraction 30min, after centrifuge separation, clear liquid Rotary Evaporators 38 DEG C with
Under be concentrated into 10mL；
(3) prepared by yeast vector: saccharomycete Liquid Culture is conventionally carried out, saccharomycete is collected and wash, it is cold
It is lyophilized dry；
(4) saccharomycete adsorbs ferrous salt: yeast vector obtained in 0.050g step (3) is weighed in 100mL beaker,
3.0mL 16.5gL is added-1FeSO4·7H2O solution is placed in 25 DEG C of water-baths with 200rmin-1Revolving speed stirring
30min, in 3000rmin-1It is centrifuged 10min under revolving speed, obtains the yeast vector for being adsorbed with ferrous salt；
(5) prepared by Nanoscale Iron: under lasting stirring or oscillating condition, the ferment for being adsorbed with ferrous salt that obtains toward step (4)
The leaf tobacco extract that step (2) obtain is added in female bacterium, continues stirring or oscillating reactions 3h, in 3000rmin-1Under the conditions of from
Heart 5min, solid carry Nanoscale Iron after freeze-drying grinding with second distillation water washing 3 times up to saccharomycete.
Embodiment 2:
Tobacco leaf processing, leaf tobacco extract and yeast vector preparation, saccharomycete absorption ferrous salt and Nanoscale Iron preparation step
With embodiment 1, in addition to weighed yeast vector is 0.020g in step (4).
Embodiment 3:
Tobacco leaf processing, leaf tobacco extract and yeast vector preparation, saccharomycete absorption ferrous salt and Nanoscale Iron preparation step
With embodiment 1, in addition to weighed yeast vector is 0.100g in step (4).
Embodiment 4:
Tobacco leaf processing, leaf tobacco extract and yeast vector preparation, saccharomycete absorption ferrous salt and Nanoscale Iron preparation step
With embodiment 1, in addition to weighed yeast vector is 0.200g in step (4).
Embodiment 5:
Tobacco leaf processing, leaf tobacco extract and yeast vector preparation, saccharomycete absorption ferrous salt and Nanoscale Iron preparation step
With embodiment 1, in addition to weighed yeast vector is 0.300g in step (4).
Embodiment 6:
Tobacco leaf processing, leaf tobacco extract and yeast vector preparation, saccharomycete absorption ferrous salt and Nanoscale Iron preparation step
With embodiment 1, in addition to weighed quality of tobacco is 0.20g in step (2).
Embodiment 7:
Tobacco leaf processing, leaf tobacco extract and yeast vector preparation, saccharomycete absorption ferrous salt and Nanoscale Iron preparation step
With embodiment 1, in addition to weighed quality of tobacco is 0.30g in step (2).
Embodiment 8:
Tobacco leaf processing, leaf tobacco extract and yeast vector preparation, saccharomycete absorption ferrous salt and Nanoscale Iron preparation step
With embodiment 1, in addition to weighed quality of tobacco is 0.40g in step (2).
Embodiment 9:
Tobacco leaf processing, leaf tobacco extract and yeast vector preparation, saccharomycete absorption ferrous salt and Nanoscale Iron preparation step
With embodiment 1, in addition to what is be added in step (2) is 10mL50% ethanol solution.
Embodiment 10:
Tobacco leaf processing, leaf tobacco extract and yeast vector preparation, saccharomycete absorption ferrous salt and Nanoscale Iron preparation step
With embodiment 1, in addition to what is be added in step (2) is 20mL50% ethanol solution.
Embodiment 11:
Tobacco leaf processing, leaf tobacco extract and yeast vector preparation, saccharomycete absorption ferrous salt and Nanoscale Iron preparation step
With embodiment 1, in addition to what is be added in step (2) is 40mL50% ethanol solution.
Embodiment 12:
Tobacco leaf processing, leaf tobacco extract and yeast vector preparation, saccharomycete absorption ferrous salt and Nanoscale Iron preparation step
With embodiment 1, in addition to the time of ultrasonic extraction in step (2) is 10min.
Embodiment 13:
Tobacco leaf processing, leaf tobacco extract and yeast vector preparation, saccharomycete absorption ferrous salt and Nanoscale Iron preparation step
With embodiment 1, in addition to the time of ultrasonic extraction in step (2) is 20min.
Embodiment 14:
Tobacco leaf processing, leaf tobacco extract and yeast vector preparation, saccharomycete absorption ferrous salt and Nanoscale Iron preparation step
With embodiment 1, in addition to the time of ultrasonic extraction in step (2) is 40min.
Embodiment 15:
Tobacco leaf processing, leaf tobacco extract and yeast vector preparation, saccharomycete absorption ferrous salt and Nanoscale Iron preparation step
With embodiment 1, in addition to being to be shaken in 40 DEG C of water-baths and extract 1h instead of ultrasonic extraction 30min in step (2).
Embodiment 16:
Tobacco leaf processing, leaf tobacco extract and yeast vector preparation, saccharomycete absorption ferrous salt and Nanoscale Iron preparation step
With embodiment 1, in addition to being to be shaken in 40 DEG C of water-baths and extract 4h instead of ultrasonic extraction 30min in step (2).
Embodiment 17:
Tobacco leaf processing, leaf tobacco extract and yeast vector preparation, saccharomycete absorption ferrous salt and Nanoscale Iron preparation step
With embodiment 1, in addition to being to replace 50% ethanol solution with 80% ethyl alcohol (volume ratio) in step (2).
Embodiment 18:
Tobacco leaf processing, leaf tobacco extract and yeast vector preparation, saccharomycete absorption ferrous salt and Nanoscale Iron preparation step
With embodiment 1, in addition to being to replace 50% ethanol solution with water in step (2).
Embodiment 19:
Tobacco leaf processing, leaf tobacco extract and yeast vector preparation, saccharomycete absorption molysite and Nanoscale Iron preparation step are same
Embodiment 1, in addition to being with FeCl in step (4)3·6H2O solution replaces FeSO4·7H2O solution.
Application Example 1:
Heavy metal pollution of soil renovation agent is used for lead-contaminated soil reparation.
The restorative procedure of lead-contaminated soil is as follows: surface layer 0-20cm Tobacco-rice rotation topsoil is taken, after natural air drying,
2mm sieve is crossed, by 500mg Pb2+·kg-1Soil is added, and after conventional method aging 1 month, is separately added into 5% soil weight
Metallic pollution renovation agent, the rice hull carbon load Nanoscale Iron of using similar approach preparation and the saccharomycete and rice husk of unsupported Nanoscale Iron
Charcoal fills basin after mixing, each processing is repeated 3 times, and routinely earth culture method is managed at room temperature.It is sampled after 3 months, it is natural
Air-dry after, measure soil in weak acid extractable, can reduction-state, oxidable state and residual form lead content.Soil physics and chemistry substantially
Matter is as shown in table 1.
Table 1 is for trying soil labile organic matter
Compared with the control group for not applying renovation agent, apply 5% saccharomycete carry Nanoscale Iron repair process 3 months after, lead
In contaminated soil weak acid extractable with can reduction-state lead content respectively lower than control group 52.52% and 15.03%, and oxidable state
It is higher than control group by 15.29% and 86.56% respectively with residual form lead content；And after applying saccharomycete 3 months of 5%, lead contamination
In soil weak acid extractable with can reduction-state lead content respectively lower than control group 50.05% and 12.00%, oxidable state and residue
State lead content is higher than control group by 0.15% and 69.28% respectively, sees attached drawing 1.
Show that saccharomycete and saccharomycete carry the biological effectiveness that lead in soil can be effectively reduced in Nanoscale Iron by comparing,
Promote soil in lead be converted into low activity oxidable state and inactive residual form, saccharomycete carry Nanoscale Iron will be in soil
The ability that lead is converted into residual form is 1.25 times of saccharomycete, and Nanoscale Iron load significantly improves saccharomycete to Lead Pollution in Soil
Passivation ability.
The rice hull carbon of addition 5% carries after Nanoscale Iron carries out repair process 3 months, weak acid extractable in lead-contaminated soil, can
Lower than control group 36.85%, 7.56% and 13.42%, residual form lead content compare respectively for reduction-state and oxidable state lead content
It is high by 75.05% according to organizing；And after adding rice hull carbon repair process 3 months of 5%, weak acid extractable in lead-contaminated soil can restore
State and oxidable state lead content lower than control group 43.85%, 7.45% and 3.45% respectively, residual form lead content is higher than control group
31.92%.
Show that saccharomycete carries Nanoscale Iron and receives the ability that the lead in soil is converted into residual form than rice hull carbon load by comparing
It is stronger than rice hull carbon load Nanoscale Iron to the passivation ability of Lead Pollution in Soil to illustrate that saccharomycete carries Nanoscale Iron by meter Tie Gao 15.3%.
Application Example 2:
Heavy metal pollution of soil renovation agent rehabilitating soil cadmium pollution.
The restorative procedure of cadmium pollution is as follows in soil: surface layer 0-20cm Tobacco-rice rotation topsoil is taken, through natural air drying
Afterwards, 2mm sieve is crossed, by 2mg Cd2+·kg-1Soil is added, and after conventional method aging 1 month, is separately added into 1% soil
Heavy metal pollution renovation agent, the rice hull carbon load Nanoscale Iron of using similar approach preparation and saccharomycete and the rice of unsupported Nanoscale Iron
Shell charcoal fills basin after mixing, each processing is repeated 3 times, and routinely earth culture method is managed at room temperature.It is sampled after 2 months, from
So air-dry after, measure soil in weak acid extractable, can reduction-state, oxidable state and residual form cadmium content.Soil is managed substantially
It is as shown in table 1 to change property.
After the saccharomycete of application 1% carries Nanoscale Iron 2 months, weak acid extractable and can reduction-state cadmium content in cadmium pollution soil
Respectively lower than control group 31.45% and 9.79%, oxidable state and residual form cadmium content 2.71% He higher than control group respectively
63.09%；And after applying saccharomycete 2 months of 1%, weak acid extractable in cadmium pollution soil, can reduction-state and oxidable state cadmium
Content lower than control group 26.01%, 8.21% and 2.38% respectively, residual form cadmium content is higher than control group by 4.71%, sees attached drawing
2。
Show that saccharomycete and saccharomycete carry the biological effectiveness that cadmium in soil can be effectively reduced in Nanoscale Iron by comparing,
Saccharomycete carries 13.4 times that the ability that cadmium in soil is converted into residual form is saccharomycete by Nanoscale Iron, and Nanoscale Iron load significantly improves
Passivation ability of the saccharomycete to Cadmium Pollution in Soils.
After the rice hull carbon and rice hull carbon of addition 1% carry Nanoscale Iron progress repair process 2 months, weak acid is mentioned in cadmium pollution soil
Take state cadmium content respectively lower than control group 9.08% and 9.91%, and residual form cadmium content 15.86% He higher than control group respectively
24.66%.
Show that saccharomycete carries Nanoscale Iron and converts the ability of residual form than rice husk carbon-supported nanometer for cadmium in soil by comparison
Iron is strong by 155.8%, and it is stronger than rice hull carbon load Nanoscale Iron to the passivation ability of Cadmium Pollution in Soils to illustrate that saccharomycete carries Nanoscale Iron.
Claims (8)
1. a kind of heavy metal pollution of soil renovation agent, which is characterized in that the Nanoscale Iron including yeast vector and its load,
In, Nanoscale Iron is that the saccharomycete of boron hydride preparation is replaced to carry Nanoscale Iron with waste tobacco leaf extract.It is mixed well withed the soil, it can
Promote lead and cadmium in soil to be converted into residual form, achievees the purpose that the biological effectiveness for reducing lead and cadmium in soil.
2. the preparation method of heavy metal pollution of soil renovation agent described in claim 1, which is characterized in that including preparing step as follows
It is rapid:
(1) tobacco leaf is handled: after the tobacco natural for having washed off impurity is dried, being dried at 50 DEG C, be crushed 200 meshes, seals
For use；
(2) prepared by leaf tobacco extract: being according to mass volume ratio by tobacco leaf powder obtained in above-mentioned steps (1) and Extraction solvent
1:5~1:40 (unit gmL-1), it is added in 250mL conical flask, 10~40min of ultrasonic extraction or shaken at room temperature extract 1
~8h, after centrifuge separation, clear liquid Rotary Evaporators are concentrated into 10mL at 38 DEG C or less；
(3) prepared by yeast vector: resulting saccharomycete is centrifuged by culture yeasts bacterium according to a conventional method, after washing, freeze-drying
Obtain yeast vector；
(4) saccharomycete absorption ferrous salt or molysite: ferrous salt or molysite aqueous solution are added to the saccharomycete that step (3) obtains and carried
In body, in 50~200rmin-10.5~2h of lower stirring or oscillation after centrifugation, washing, obtains being adsorbed with ferrous salt or molysite
Saccharomycete；
(5) prepared by Nanoscale Iron: under conditions of lasting stirring or oscillation, being adsorbed with ferrous salt or molysite toward what step (4) obtained
Saccharomycete in step (2) obtained leaf tobacco extract is added, continue stirring or oscillating reactions 0.5h~8h, then carry out solid-liquid point
From solid carries Nanoscale Iron after freeze-drying grinding with second distillation water washing 3 times up to saccharomycete.
3. the preparation method of heavy metal pollution of soil renovation agent according to claim 2, which is characterized in that the saccharomycete
For Saccharomyces cerevisiae or saccharomyces cerevisiae.
4. the preparation method of heavy metal pollution of soil renovation agent according to claim 2, which is characterized in that in step (2)
The Extraction solvent is the ethanol solution of 0~80% (volume ratio).
5. the preparation method of heavy metal pollution of soil renovation agent according to claim 2, which is characterized in that in step (4)
The yeast vector quality is that the mass ratio of 0.02~1.0g, the ferrous salt or molysite and yeast vector is 1:2
~30.
6. the preparation method of heavy metal pollution of soil renovation agent according to claim 2, which is characterized in that step (5) exists
With 0.5~0.8mLmin while stirring or oscillation-1Gas supply speed into the solution lead to N2, the separation of solid and liquid is
In 3000~5000rmin-15~10min is centrifuged under revolving speed.
7. heavy metal pollution of soil renovation agent described in claim 1, or pass through side described in any one of claim 2~6
The heavy metal pollution of soil renovation agent that method is prepared is used for the reparation of lead-contaminated soil.
8. heavy metal pollution of soil renovation agent described in claim 1, or pass through side described in any one of claim 2~6
The heavy metal pollution of soil renovation agent that method is prepared is used for the reparation of cadmium pollution soil.
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