CN109758613A - A kind of 3 D-printing combines biological three-dimensional printing technology to prepare tendon method - Google Patents
A kind of 3 D-printing combines biological three-dimensional printing technology to prepare tendon method Download PDFInfo
- Publication number
- CN109758613A CN109758613A CN201910073912.4A CN201910073912A CN109758613A CN 109758613 A CN109758613 A CN 109758613A CN 201910073912 A CN201910073912 A CN 201910073912A CN 109758613 A CN109758613 A CN 109758613A
- Authority
- CN
- China
- Prior art keywords
- printing
- tendon
- cell
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention belongs to biological three-dimensional printing technology fields, in particular a kind of 3 D-printing combines biological three-dimensional printing technology to prepare tendon method, preparation method are as follows: the culture of S1, seed cell, the preparation of S2, tendon scaffold, S3, cell coated preparation and the cell coated processing of S4, nano fibrous membrane-, tendon stem cell is the preservation of this laboratory, is incubated in incubator with the DMEM culture medium containing 10% fetal calf serum；The present invention, the 3 D-printing combines biological three-dimensional printing technology, easily controllable fibre diameter obtains tendon scaffold, and obtains lubricant cell coating in its printout surface by biological three-dimensional printing technology, make itself just to contain the seed cell being largely colonized, conducive to cell Proliferation, differentiation, secretion matrix, and there is favorable lubricating property, the production method is simple and convenient, preferably bionical natural tendon tissue form, cell component and greasy property, are conducive to tendon repair and normal function restores.
Description
Technical field
The invention belongs to biological three-dimensional printing technology fields, and in particular to a kind of biological 3 D-printing skill of 3 D-printing combination
Art prepares tendon method.
Background technique
Tendon injury is one of most common injury gained in sports, and the common type in clinical soft tissue injury, current
Treatment method there is certain limitation or deficiency, bracket and seed cell the regeneration of damaged tissues is guided jointly and
The structure of regenerating tissues is controlled, is the key that determine whether artificial tendon can be used for clinical treatment；
Currently, preparing tendon scaffold mainly includes weaving class bracket with fibre bundle bracket, it is knitted class bracket, electrostatic spinning
Several classes such as nano fiber scaffold, however the tendon scaffold of above method preparation, seed cell sticks, is colonized efficiency in reparation
Lowly, it is difficult to achieve the effect that quickly to repair injury tissue, moreover, obtained tendon scaffold is in vivo easily and surrounding tissue
It is adhered, the recovery of the normal function after influencing tendon rehabilitation.
Summary of the invention
To solve the problems mentioned above in the background art.The present invention provides a kind of 3 D-printings to combine biology is three-dimensional to beat
Print technology prepares tendon method, has the characteristics that be conducive to tendon repair and normal function is abundant.
To achieve the above object, the invention provides the following technical scheme: a kind of 3 D-printing combines biological 3 D-printing skill
Art prepares tendon method, preparation method are as follows:
The culture of S1, seed cell；
The preparation of S2, tendon scaffold；
S3, cell coated preparation.
Preferably, in the S1 step seed cell culture, tendon stem cell with containing 10% fetal calf serum DMEM train
Feeding base is incubated in incubator, and stem cell and fat stem cell are to contain 10% fetal calf serum, Connective Tissue Growth Factor
CTGF25ng/ml, ascorbic acid 25uM α-MEM culture medium be incubated in incubator.
Preferably, contain 5%CO in incubator used in the S1 step2, the temperature in incubator is 37 DEG C.
Preferably, in the S2 step preparation step of tendon scaffold include S21, model foundation: using 3 D-printing
Software establishes printer model, is single-layer or multi-layer oblong-shaped, and save, and waits next step；
The preparation of S22, material: measuring suitable bioabsorbable polymer material, waits next step；
The preparation of S23, equipment: the print parameters of adjustment melting electrostatic spinning 3D printing equipment, by the biology of step S22
High molecular material is placed in inside the barrel of melting electrostatic spinning 3D printing equipment, and it is three-dimensional that starting device carries out melting electrostatic spinning
Printing obtains tendon scaffold, waits next step；
Preferably, the print parameters of printing device are in the S23 step, and print head diameter is 150-400 μm, printing temperature
Degree is 120-200 DEG C, and barrel air pressure is 600-1000KPa in print procedure, and melt spinning negative high voltage module voltage is -2-
10kV, print structure are controlled by printing path, and printing path is 0/90 °, 0/60 ° and 0/60/120 °.
Preferably, preparation step cell coated in the S3 step includes the foundation of S31, model: using 3 D-printing
Software establishes printer model, is single-layer or multi-layer oblong-shaped, and save.
The preparation of S32, material: it measures suitable sterile natural material and is mixed with seed cell, obtained containing Cellular gels, so
After take part to be mixed with suitable lubrication related substances containing Cellular gels.
S33, cell coated printing: 3D printing equipment is sterilized, and sets print parameters；
S34, by being added containing Cellular gels to the barrel of 3D printing equipment in step S32, with nano fibrous membrane
It receives, starts print routine, obtain nano fibrous membrane cell coated, the Cellular gels containing greasing substance in S32 are added
To the barrel of 3D printing equipment, same print parameters are printed, and are printed on cell coated other adjacent area, are used appropriate side
Method is crosslinked gel.
Preferably, the print parameters in the S33 step are that the print head diameter of 3D printing equipment is 150-400 μm,
Print temperature is 18-37 DEG C, and barrel air pressure is 600-1000KPa in print procedure, and print structure is controlled by printing path, is printed
Path is 0/90 °, 0/60 ° and 0/60/120 °.
Preferably, preparation method further includes the cell coated processing of S4, nano fibrous membrane-.
Preferably, the cell coated processing step of nano fibrous membrane-includes that will obtain in S34 step in the S3 step
Have cell coated nano fibrous membrane to be crimped, and to be located at outer layer with the cell coated of lubrication related substances, obtains people
Work tendon, length 2-10cm, diameter 2-10mm cylindric in coiled structure.
Compared with prior art, the beneficial effects of the present invention are:
The present invention, the 3 D-printing combine biological three-dimensional printing technology, and easily controllable fibre diameter obtains tendon scaffold,
And lubricant cell coating is obtained in its printout surface by biological three-dimensional printing technology, make itself just to contain the kind being largely colonized
Daughter cell is conducive to cell Proliferation, differentiation and secretion matrix, and has favorable lubricating property, and the production method is simple and convenient, more preferably
The bionical natural tendon tissue form in ground, cell component and greasy property, are conducive to tendon repair and normal function restores.
Detailed description of the invention:
Fig. 1 is the flow diagram of artificial tendon of the present invention；
Fig. 2 is the schematic diagram data of control experiment of the present invention；
Fig. 3 is the result schematic diagram of qRT-PCR of the present invention detection；
Fig. 4 is stress-strain diagram schematic diagram of the present invention；
Fig. 5 is friction coefficient vs lab diagram of the present invention.
Specific embodiment
Below in conjunction with attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that institute
The embodiment of description is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention,
Every other embodiment obtained by those of ordinary skill in the art without making creative efforts, belongs to this hair
The range of bright protection.
The present invention the following technical schemes are provided: a kind of 3 D-printing combines biological three-dimensional printing technology to prepare tendon method,
Preparation method are as follows:
The culture of S1, seed cell；
The preparation of S2, tendon scaffold；
S3, cell coated preparation.
Specifically, in the S1 step seed cell culture, tendon stem cell with containing 10% fetal calf serum DMEM train
Feeding base is incubated in incubator, and stem cell and fat stem cell are to contain 10% fetal calf serum, Connective Tissue Growth Factor
CTGF25ng/ml, ascorbic acid 25uM α-MEM culture medium be incubated in incubator.
Specifically, containing 5%CO in incubator used in the S1 step2, the temperature in incubator is 37 DEG C.
Specifically, the preparation step of tendon scaffold includes the foundation of S21, model in the S2 step: using 3 D-printing
Software establishes printer model, is single-layer or multi-layer oblong-shaped, and save, and waits next step；
The preparation of S22, material: measuring suitable bioabsorbable polymer material, waits next step；
The preparation of S23, equipment: the print parameters of adjustment melting electrostatic spinning 3D printing equipment, by the biology of step S22
High molecular material is placed in inside the barrel of melting electrostatic spinning 3D printing equipment, and it is three-dimensional that starting device carries out melting electrostatic spinning
Printing obtains tendon scaffold, waits next step；
Specifically, the print parameters of printing device are in the S23 step, print head diameter is 150-400 μm, printing temperature
Degree is 120-200 DEG C, and barrel air pressure is 600-1000KPa in print procedure, and melt spinning negative high voltage module voltage is -2-
10kV, print structure are controlled by printing path, and printing path is 0/90 °, 0/60 ° and 0/60/120 °.
Specifically, preparation step cell coated in the S3 step includes the foundation of S31, model: using 3 D-printing
Software establishes printer model, is single-layer or multi-layer oblong-shaped, and save.
The preparation of S32, material: it measures suitable sterile natural material and is mixed with seed cell, obtained containing Cellular gels, so
After take part to be mixed with suitable lubrication related substances containing Cellular gels.
S33, cell coated printing: 3D printing equipment is sterilized, and sets print parameters；
S34, by being added containing Cellular gels to the barrel of 3D printing equipment in step S32, with nano fibrous membrane
It receives, starts print routine, obtain nano fibrous membrane cell coated, the Cellular gels containing greasing substance in S32 are added
To the barrel of 3D printing equipment, same print parameters are printed, and are printed on cell coated other adjacent area, are used appropriate side
Method is crosslinked gel.
Specifically, the print parameters in the S33 step are, the print head diameter of 3D printing equipment is 150-400 μm,
Print temperature is 18-37 DEG C, and barrel air pressure is 600-1000KPa in print procedure, and print structure is controlled by printing path, is printed
Path is 0/90 °, 0/60 ° and 0/60/120 °.
Specifically, preparation method further includes the cell coated processing of S4, nano fibrous membrane-.
Specifically, the cell coated processing step of nano fibrous membrane-includes that will obtain in S34 step in the S3 step
Have cell coated nano fibrous membrane to be crimped, and to be located at outer layer with the cell coated of lubrication related substances, obtains people
Work tendon, length 2-10cm, diameter 2-10mm cylindric in coiled structure.
Embodiment 1
(1) by inside the barrel of high molecular material polycaprolactone merging melting electrostatic spinning 3D printing equipment, adjustment is molten
Melt the technological parameter of electrostatic spinning 3D printing equipment: print head diameter is 200 μm, and print temperature is 180 DEG C, in print procedure
Barrel air pressure is 600-1000KPa, and melt spinning negative high voltage module voltage is -3kV, and print structure is controlled by printing path, beaten
Printing path is 0/90 °, and starting device carries out melting electrostatic spinning 3 D-printing and obtains tendon scaffold.
(2) tendon stem cell is mixed with 10g sodium alginate gel, is added in the barrel of three-dimensional printer, printing temperature
Degree is set as 37 DEG C, and print head diameter is 200 μm, and a height of 200 μm of layer, barrel air pressure is 800KPa.Printing path is set as
0/90 °, resulting nanofiber film surface is printed, start print routine, print single layer rectangular support frame, length is
3cm, width 1cm.
(3) tendon stem cell, cell factor BMP7 are mixed with 10g sodium alginate gel, is added to three-dimensional printer
In barrel, print temperature is set as 18-37 DEG C, and print head diameter is 200 μm, and a height of 200 μm of layer, barrel air pressure is
800KPa, printing path are set as 0/90 °, in resulting nanofiber film surface, are printed with print area adjacent area,
Start print routine, prints single layer rectangular support frame, length 2cm, width 1cm are finally obtained with greasy property cell
The nano fibrous membrane of coating.
(4) nano fibrous membrane is crimped along broadside, obtains cylindrical artificial tendon, the length is 5cm, diameter is
8mm, detailed process such as Fig. 1.
Embodiment 2
It by gained artificial tendon, is put into incubator and cultivates, with the nano fibrous membrane that common electrostatic spinning obtains, surface kind
It plants tendon stem cell to compare for control group, after cultivating 1,3,5,7 and 9 day respectively, utilizes the flesh in mtt assay detection tendon scaffold
Tendon cell vigor, result figure as indicated with 2, show the flesh that melting electrostatic spinning 3 D-printing combines biological three-dimensional printing technology to obtain
Tenocyte cell on tendon bracket has good proliferation behavior, and cell viability is preferable, and more significant compared with control group proliferation behavior.
Embodiment 3
Obtained tendon scaffold is subjected to qRT-PCR detection, the artificial tendon for having greasy property cell coated gained,
It is put into incubator and cultivates, made with the common electrostatic spinning film surface of resulting tendon scaffold and single layer plantation tendon stem cell
Comparison after cultivating 7 days respectively, carries out qRT-PCR detection, as indicated at 3, two groups of display have cell coated tendon scaffold to result figure
On Tenocyte cell tendon associated products (COLI, COLIII, SCX, TNMD) expression it is obvious more, while having greasy property thin
It is obvious less that the tendon scaffold of born of the same parents' coating sticks associated products (Vinculin) expression.
Embodiment 4
Tendon scaffold obtained in above-described embodiment 1 is placed in progress mechanical stretch test, stress on omnipotent test machine
For strain curve as shown in figure 4, the maximum fracture strength of the bracket is 177.433MPa as the result is shown, mechanical property is preferable.
Embodiment 5
By to artificial tendon scaffold be placed on PVvalue testing machine and carry out tribology tester, to be free of greasing substance
Artificial tendon as control, as a result as shown in figure 5, the artificial tendon coefficient of friction is significantly less than control group as the result is shown.
Finally, it should be noted that the foregoing is only a preferred embodiment of the present invention, it is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features.
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in of the invention
Within protection scope.
Claims (9)
1. a kind of 3 D-printing combines biological three-dimensional printing technology to prepare tendon method, preparation method are as follows:
The culture of S1, seed cell；
The preparation of S2, tendon scaffold；
S3, cell coated preparation.
2. a kind of 3 D-printing according to claim 1 combines biological three-dimensional printing technology to prepare tendon method, feature
Be: the culture of seed cell in the S1 step, tendon stem cell are incubated at the DMEM culture medium containing 10% fetal calf serum
In incubator, stem cell and fat stem cell are to contain 10% fetal calf serum, Connective Tissue Growth Factor CTGF25ng/ml, resist
α-MEM the culture medium of bad hematic acid 25uM is incubated in incubator.
3. a kind of 3 D-printing according to claim 2 combines biological three-dimensional printing technology to prepare tendon method, feature
It is: contains 5%CO in incubator used in the S1 step2, the temperature in incubator is 37 DEG C.
4. a kind of 3 D-printing according to claim 1 combines biological three-dimensional printing technology to prepare tendon method, feature
Be: the preparation step of tendon scaffold includes the foundation of S21, model in the S2 step: being beaten using the foundation of 3 D-printing software
Stamp type is single-layer or multi-layer oblong-shaped, and saves, and waits next step；
The preparation of S22, material: measuring suitable bioabsorbable polymer material, waits next step；
The preparation of S23, equipment: the print parameters of adjustment melting electrostatic spinning 3D printing equipment, by the biological high score of step S22
Inside the barrel of sub- material merging melting electrostatic spinning 3D printing equipment, starting device carries out melting electrostatic spinning 3 D-printing
Tendon scaffold is obtained, next step is waited.
5. a kind of 3 D-printing according to claim 4 combines biological three-dimensional printing technology to prepare tendon method, feature
Be: the print parameters of printing device are in the S23 step, and print head diameter is 150-400 μm, print temperature 120-
200 DEG C, barrel air pressure is 600-1000KPa in print procedure, and melt spinning negative high voltage module voltage is -2-10kV, printing knot
Structure is controlled by printing path, and printing path is 0/90 °, 0/60 ° and 0/60/120 °.
6. a kind of 3 D-printing according to claim 1 combines biological three-dimensional printing technology to prepare tendon method, feature
Be: cell coated preparation step includes the foundation of S31, model in the S3 step: being beaten using the foundation of 3 D-printing software
Stamp type is single-layer or multi-layer oblong-shaped, and saves.
The preparation of S32, material: measuring suitable sterile natural material and mix with seed cell, obtains then taking containing Cellular gels
Part is mixed containing Cellular gels with suitable lubrication related substances.
S33, cell coated printing: 3D printing equipment is sterilized, and sets print parameters；
S34, by being added containing Cellular gels to the barrel of 3D printing equipment in step S32, received with nano fibrous membrane,
Start print routine, obtains nano fibrous membrane cell coated, the Cellular gels containing greasing substance in S32 are added to three-dimensional
The barrel of printing device, same print parameters are printed, and cell coated other adjacent area are printed on, using proper method to solidifying
Glue is crosslinked.
7. a kind of 3 D-printing according to claim 6 combines biological three-dimensional printing technology to prepare tendon method, feature
Be: the print parameters in the S33 step are that the print head diameter of 3D printing equipment is 150-400 μm, and print temperature is
18-37 DEG C, barrel air pressure is 600-1000KPa in print procedure, and print structure is controlled by printing path, printing path 0/
90 °, 0/60 ° and 0/60/120 °.
8. a kind of 3 D-printing according to claim 6 combines biological three-dimensional printing technology to prepare tendon method, feature
Be: preparation method further includes the cell coated processing of S4, nano fibrous membrane-.
9. combine biological three-dimensional printing technology to prepare tendon method a kind of 3 D-printing according to claim 8, it is special
Sign is: the cell coated processing step of nano fibrous membrane-includes applying the tool cell obtained in S34 step in the S3 step
Layer nano fibrous membrane is crimped, and to be located at outer layer with the cell coated of lubrication related substances, is obtained artificial tendon, is in
Coiled structure is cylindric, length 2-10cm, diameter 2-10mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910073912.4A CN109758613A (en) | 2019-01-25 | 2019-01-25 | A kind of 3 D-printing combines biological three-dimensional printing technology to prepare tendon method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910073912.4A CN109758613A (en) | 2019-01-25 | 2019-01-25 | A kind of 3 D-printing combines biological three-dimensional printing technology to prepare tendon method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109758613A true CN109758613A (en) | 2019-05-17 |
Family
ID=66455307
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910073912.4A Pending CN109758613A (en) | 2019-01-25 | 2019-01-25 | A kind of 3 D-printing combines biological three-dimensional printing technology to prepare tendon method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109758613A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115845136A (en) * | 2022-12-15 | 2023-03-28 | 南京市第一医院 | Near-field direct-writing electrostatic spinning 3D bionic tendon bone repair support and preparation method thereof |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101829361A (en) * | 2009-03-10 | 2010-09-15 | 广州迈普再生医学科技有限公司 | Nano-bionic material for tissue repair and preparation method thereof |
| US20150024493A1 (en) * | 2013-07-16 | 2015-01-22 | Nanyang Technological University | Method for preparing a patterned substrate and use thereof in implants for tissue engineering |
| US20160184480A1 (en) * | 2014-12-31 | 2016-06-30 | Daniel Cox | Crosslinkable 3d printed biomaterial-based implants and methods of manufacture thereof |
| CN106659148A (en) * | 2014-05-12 | 2017-05-10 | 鲁斯特生物股份有限公司 | Ready-to-print cells and integrated devices |
| CN206809488U (en) * | 2016-12-30 | 2017-12-29 | 广东工业大学 | A kind of cytoskeleton preparation system |
| CN108265339A (en) * | 2018-04-03 | 2018-07-10 | 西北工业大学 | For printing electrostatic spinning system and method perpendicular to direction of an electric field densification plane |
| CN108404213A (en) * | 2018-05-14 | 2018-08-17 | 上海交通大学医学院附属第九人民医院 | It is a kind of to prepare tendon scaffold method using 3 D-printing and electrostatic spinning technique |
| CN108404205A (en) * | 2018-07-04 | 2018-08-17 | 上海交通大学医学院附属第九人民医院 | A kind of biological 3 D printing preparation method thereof of the load anti-inflammatory Meniscus scaffold of cartilage cell |
-
2019
- 2019-01-25 CN CN201910073912.4A patent/CN109758613A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101829361A (en) * | 2009-03-10 | 2010-09-15 | 广州迈普再生医学科技有限公司 | Nano-bionic material for tissue repair and preparation method thereof |
| US20150024493A1 (en) * | 2013-07-16 | 2015-01-22 | Nanyang Technological University | Method for preparing a patterned substrate and use thereof in implants for tissue engineering |
| CN106659148A (en) * | 2014-05-12 | 2017-05-10 | 鲁斯特生物股份有限公司 | Ready-to-print cells and integrated devices |
| US20160184480A1 (en) * | 2014-12-31 | 2016-06-30 | Daniel Cox | Crosslinkable 3d printed biomaterial-based implants and methods of manufacture thereof |
| CN206809488U (en) * | 2016-12-30 | 2017-12-29 | 广东工业大学 | A kind of cytoskeleton preparation system |
| CN108265339A (en) * | 2018-04-03 | 2018-07-10 | 西北工业大学 | For printing electrostatic spinning system and method perpendicular to direction of an electric field densification plane |
| CN108404213A (en) * | 2018-05-14 | 2018-08-17 | 上海交通大学医学院附属第九人民医院 | It is a kind of to prepare tendon scaffold method using 3 D-printing and electrostatic spinning technique |
| CN108404205A (en) * | 2018-07-04 | 2018-08-17 | 上海交通大学医学院附属第九人民医院 | A kind of biological 3 D printing preparation method thereof of the load anti-inflammatory Meniscus scaffold of cartilage cell |
Non-Patent Citations (3)
| Title |
|---|
| JOYDIP KUNDU ET AL: ""An additive manufacturing-based PCL-alginate-chondrocyte bioprinted scaffold for cartilage tissue engineering"", 《JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE》 * |
| 王哲: ""激光熔融静电纺丝法制备组织工程支架及其性能研究"", 《万方数据》 * |
| 鞠尔男等: ""生物医疗领域三维打印的研究与应用"", 《中国组织工程研究》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115845136A (en) * | 2022-12-15 | 2023-03-28 | 南京市第一医院 | Near-field direct-writing electrostatic spinning 3D bionic tendon bone repair support and preparation method thereof |
| CN115845136B (en) * | 2022-12-15 | 2024-01-26 | 南京市第一医院 | Near-field direct-writing electrostatic spinning 3D bionic tendon-bone repair stent and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Castilho et al. | Bi-layered micro-fibre reinforced hydrogels for articular cartilage regeneration | |
| EP1185211B1 (en) | Bioengineered anterior cruciate ligament | |
| CN109701083A (en) | It is a kind of to prepare artificial tendon method using biological 3 D-printing and electrostatic spinning technique | |
| Zheng et al. | Use of chitosan conduit combined with bone marrow mesenchymal stem cells for promoting peripheral nerve regeneration | |
| CN105749350B (en) | A kind of cardiac muscle sticking patch and preparation method thereof | |
| Puetzer et al. | The effect of the duration of mechanical stimulation and post-stimulation culture on the structure and properties of dynamically compressed tissue-engineered menisci | |
| Rubbens et al. | Intermittent straining accelerates the development of tissue properties in engineered heart valve tissue | |
| US20060134158A1 (en) | Chitosan/acidic biopolymer hybrid fiber and culture base for animal cells | |
| Park et al. | Characteristics of tissue-engineered cartilage from human auricular chondrocytes | |
| CN109646715A (en) | Electrospinning 3D printing prepares the multilayer cartilage complex of factor-containing microballoon | |
| CN109821072A (en) | It is a kind of to prepare tendon synostosis three-phase bracket with coaxial spinning using melting electrospinning 3 D-printing | |
| Chen et al. | Engineering superficial zone features in tissue engineered cartilage | |
| CN106730010A (en) | Acellular nerve hydrogel is used to prepare the purposes of peripheral nerve injury remediation composition | |
| CN109758613A (en) | A kind of 3 D-printing combines biological three-dimensional printing technology to prepare tendon method | |
| CN110507853A (en) | A kind of method of biological 3 D-printing interverbebral disc in support liquid medium | |
| CN109837215A (en) | Melt the tendon synostosis three-phase bracket of electrospinning 3 D-printing preparation | |
| Song et al. | Corneal bioprinting using a high concentration pure collagen I transparent bioink | |
| CN109701079A (en) | Electrospinning 3D printing prepares the cartilage complex of multilayer containing coaxial electrostatic spinning | |
| CN106834223A (en) | Method from inducing umbilical cord mesenchymal stem to Chondrocyte Differentiation | |
| CN111282021B (en) | Meniscus composite scaffold and preparation method thereof | |
| CN106943630B (en) | Hyaline cartilage-like massive tissue cultured and grown in vitro and preparation method and application thereof | |
| CN106606804A (en) | Preparation method of composite structure | |
| CN110218344A (en) | A kind of elastic hydrogel fiber and its preparation method and application | |
| CN106110405A (en) | A kind of functionalization guides muscular tissue regeneration membrane and its preparation method and application | |
| Kistamás et al. | Multifactorial approaches to enhance maturation of human iPSC-derived cardiomyocytes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190517 |