CN206809488U - A kind of cytoskeleton preparation system - Google Patents
A kind of cytoskeleton preparation system Download PDFInfo
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- CN206809488U CN206809488U CN201621493047.7U CN201621493047U CN206809488U CN 206809488 U CN206809488 U CN 206809488U CN 201621493047 U CN201621493047 U CN 201621493047U CN 206809488 U CN206809488 U CN 206809488U
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- syringe pump
- shower nozzle
- cytoskeleton
- spray printing
- collection device
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Abstract
The utility model discloses a kind of cytoskeleton preparation system, including：For receiving the collection device of spray printing material；For the movable platform for driving collection device to move；For spraying spray printing material to form the shower nozzle of syringe pump first of cell cytoskeleton, the shower nozzle of syringe pump first is connected to provide thermal source to produce the heater of the spray printing material of molten condition and high-pressure electrostatic field device, and high-pressure electrostatic field device is used to make the spray printing material in the shower nozzle of syringe pump first under high temperature fused state spray to collection device；High-pressure electrostatic field device is connected with computer, and the shower nozzle of syringe pump first is arranged at the top of collection device；For spraying the shower nozzle of syringe pump second of biology ink to cell cytoskeleton, the shower nozzle of syringe pump second is arranged at the top of collection device；Computer, for controlling the successively injection of the shower nozzle of syringe pump first and the shower nozzle of syringe pump second.The utility model can make to form the more accurate cytoskeleton of structure.
Description
Technical field
Tissue engineering technique field is the utility model is related to, more specifically to a kind of cytoskeleton preparation system.
Background technology
The national science foundation of the US committee proposes the definition of organizational project：, will based on the relation between structure and function
The general principle of engineering science and life science is applied among the normal and pathological tissue of mammal, for recovering, maintain or
Person improves the development of the biological substitution product of function of organization.Forming the three elements of organizational project includes：Cell, support, growth factor.
Cell is the most basic structural units of all living things tissue, and support provides support for cells grown for a complete tissue, raw
The long factor can guide, coordinate cellular activity.
At present, in terms of degradation speed matches with cell growth breeding in control cytoskeleton body；In control support tool
The three-dimensional configuration configuration aspects that require and all also deposit in terms of the bioactivity-protected and control release to growth factor etc.
It is unresolved in many stubborn problems.
Support has vital effect in organizational project, need to possess following feature：
The tridimensional network connected with higher porosity with inside, the offer strong point can be sticked for cell,
And it is easy to the transport of nutriment and metabolic waste；, can with good biocompatibility, controllable degradability and absorbability
It is processed as three-dimensional structure；With appropriate surface chemical property, in favor of the sticking of cell, breed, break up；Can be according to different groups
The requirement knitted, regulate and control suitable mechanical property.
At present, conventional tissue engineering cell scaffold preparation technology group will be divided into pore method and Freeze Drying Technique and electrostatic
Spinning technique three major types.
Pore method has that mechanical strength is low, the two serious problems of residual of pore creating material, and the residual of pore creating material can influence carefully
The attachment of born of the same parents is with growth so as to causing tissue to ruin death.
The three-dimensional rack hole less shortcoming of size that Freeze Drying Technique can have structure causes cell smoothly to penetrate
Support reaches its inside configuration, still has more cell adherence in rack surface, normal proliferative and tissue of this situation to cell
Comprehensive reparation range of decrease have adverse effect on；And the residual of organic solvent can influence the culture of bioactie agent or cell.
Biological support prepared by the solution electrospinning of electrostatic spinning process there is it is inevitably very important the defects of, it is molten
The problems such as dissolvent residual existing for liquid method of electrostatic spinning and high cost recovery, limits its application in biomedical sector.
In summary, how to provide one kind and be easy to cytoskeleton shaped device, be that current those skilled in the art urgently solve
Certainly the problem of.
Utility model content
In view of this, the purpose of this utility model is to provide a kind of cytoskeleton preparation system, and the system can be easy to make
Make cytoskeleton.
To achieve these goals, the utility model provides following technical scheme：
A kind of cytoskeleton preparation system, including：
For receiving the collection device of spray printing material；
For the movable platform for driving collection device to move；
For spraying spray printing material to form the shower nozzle of syringe pump first of cell cytoskeleton, the shower nozzle of syringe pump first connects
For providing thermal source to produce the heater of the spray printing material of molten condition and high-pressure electrostatic field device, the high-voltage electrostatic field
Device is used to make the spray printing material in the shower nozzle of syringe pump first under high temperature fused state spray to collection device；The high pressure
Static field device is connected with computer, and the shower nozzle of syringe pump first is arranged at the top of the collection device；
For spraying the shower nozzle of syringe pump second of biology ink to the cell cytoskeleton, the shower nozzle of syringe pump second is arranged at
The top of the collection device；
Computer, for controlling the successively injection of the shower nozzle of syringe pump first and the syringe pump second shower nozzle, it is used in combination
Movement in the movable platform of control relative to the shower nozzle of syringe pump first.
Preferably, the jet-printing head of the shower nozzle of syringe pump first it is sophisticated between the collection plane of the collection device away from
It it is 0.5 to 3 millimeter from scope.
Preferably, the movable platform is horizontal shifting platform, and the horizontal shifting platform is also connected with vertical shift dress
Put, to carry out vertical shift after completing individual layer spray printing.
Preferably, the shower nozzle of syringe pump first and the syringe pump second shower nozzle is arranged on syringe pump support, institute
It is high-pressure modular to state heater, or the heater is the high-pressure electrostatic field device.
Preferably, the inside diameter ranges of the injection needle of the shower nozzle of syringe pump first are 200 microns to 250 microns.
Preferably, the spray printing material is artificial synthesized PLA high polymer materials；
And/or the computer connects the movable platform, the motion control device bag by motion control device
Include the one or more in manipulator controller, retarder control device and electromechanical controlling device.
The spray printing material of molten condition can be passed through high-pressure electrostatic by cytoskeleton preparation system provided by the utility model
The effect of field device can form electrostatic spinning, so as to form more accurate cell cytoskeleton, direct write electrostatic on collection device
Spining technology can realize the path controllable precise of material, to realize the cytoskeleton with precision architecture.
Brief description of the drawings
, below will be to embodiment in order to illustrate more clearly of the utility model embodiment or technical scheme of the prior art
Or the required accompanying drawing used is briefly described in description of the prior art, it should be apparent that, drawings in the following description are only
It is embodiment of the present utility model, for those of ordinary skill in the art, on the premise of not paying creative work, also
Other accompanying drawings can be obtained according to the accompanying drawing of offer.
Fig. 1 is a kind of connection diagram of cytoskeleton preparation system provided by the utility model.
In Fig. 1：
1 it is computer, 2 be movable platform, 3 be high-pressure electrostatic field device, 4 be double shower nozzle syringe pumps, 5 is to collect dress
Put, 6 be motion control device.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out
Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made
The every other embodiment obtained, belong to the scope of the utility model protection.
Core of the present utility model is to provide a kind of cytoskeleton preparation system, and the system can be easy to manufacture cell branch
Frame.
Fig. 1 is refer to, Fig. 1 is a kind of connection diagram of cytoskeleton preparation system provided by the utility model.
A kind of cytoskeleton preparation system provided by the utility model, structure composition on mainly include collection device 5,
Movable platform 2, the shower nozzle of syringe pump first, the shower nozzle of syringe pump second and computer 1.
Wherein, collection device 5 is used to receive spray printing material, and reception refers to receiving the shower nozzle of syringe pump first, syringe pump the
The spray printing material that two shower nozzles spray to it, and form cell cytoskeleton and final cytoskeleton, that is to say, that collection device 5 is use
In the device for setting product, it can be a container or be a planar structure.Movable platform 2 is used to drive collection device
5 move, it is mobile can specifically include moving horizontally, a variety of move modes such as vertical shift.
The shower nozzle of syringe pump first is used to spray spray printing material to collection device 5, to form the cell of spray printing material composition
Framework, the shower nozzle of syringe pump first connection heater, heater are used to provide thermal source to produce the spray printing material of molten condition,
The spray printing material of molten condition can be sprayed from the shower nozzle of syringe pump first to collection device 5, and the shower nozzle of syringe pump first is arranged at receipts
The top of acquisition means 5.Top is the relative position relation of the shower nozzle of syringe pump first and collection device 5, can be up-down structure,
Can be the relative position relation with certain level corner, so that in actual use, the shower nozzle of syringe pump first fills to collection
5 are put to be sprayed.The shower nozzle of syringe pump first is also connected with high-pressure electrostatic field device 3, and high-pressure electrostatic field device 3 is used to utilize high pressure
Electrostatic field makes the spray printing material in the shower nozzle of syringe pump first under high temperature fused state spray to collection device 5；High-voltage electrostatic field fills
3 are put to be connected with computer 1.
The shower nozzle of syringe pump second is used to spray biology ink to cell cytoskeleton, and the shower nozzle of syringe pump second is arranged at collection device 5
Top.Computer 1 is used for the successively injection for controlling the shower nozzle of syringe pump first and the shower nozzle of syringe pump second, and for controlling and can transport
Moving platform 2 relative to the shower nozzle of syringe pump first movement.
It should be noted that above-mentioned high-pressure electrostatic field device 3 is to be arranged between the shower nozzle of syringe pump first and collection device 5
Electrostatic field, can be played by caused electrostatic field makes the spray printing material in the shower nozzle of syringe pump first be sprayed onto collection device 5
Effect.
Two injection pump spray-heads are used for the spray printing material for producing molten condition and biology ink, and two injection pump spray-heads can be same
When be arranged on a double shower nozzle syringe pumps 4.Collection device 5 is used to receive spray printing material and biology ink.
It should be noted that the spray printing material of above-mentioned molten condition can be formed by the effect of high-pressure electrostatic field device 3
Electrostatic spinning, so as to form more accurate cell cytoskeleton on collection device 5, direct write electrostatic spinning technique can realize material
Path controllable precise, with realize with precision architecture cytoskeleton.
Cytoskeleton preparation system provided by the utility model can pass through the shower nozzle of syringe pump first and high-voltage electrostatic field
The electrostatic spinning technique for cooperatively forming molten condition material of device 3, and pass through the shifting of movable platform 2 drive collection device 5
It is dynamic, to form the alternation of the shower nozzle of syringe pump first and the shower nozzle of syringe pump second, the two is realized successively and print, have
Body, successively the step of printing may be referred to the description below：Syringe pump the first shower nozzle spray printing goes out cell cytoskeleton, by movable flat
The movement of platform 2 makes cell cytoskeleton be moved to below the shower nozzle of syringe pump second equipped with biology ink, and then will be biological black from syringe pump
Second shower nozzle is sprayed in the gap of cell cytoskeleton, is formed the first Rotating fields, is then made receipts again by the movement of movable platform 2
Acquisition means 5 move to the first shower nozzle of syringe pump direction, spray printing second layer cell cytoskeleton, the superposition printing circulated, last shape
Into cell-material composite.
On the basis of above-described embodiment, the jet-printing head tip of the shower nozzle of syringe pump first and the collection of collection device 5 are put down
The distance between face range L is 0.5 to 3 millimeter.The collection of the shower nozzle of syringe pump first that the present embodiment is provided and collection device 5
Spacing is maintained between 0.5 to 3 millimeter between plane, has minimum jet length, can ensure high voltage electrostatic field
On the premise of, further ensure spray printing effect.
On the basis of above-described embodiment, movable platform 2 is specifically as follows horizontal shifting platform, horizontal shifting platform
Vertical shift device is also connected with, to carry out vertical shift after completing individual layer spray printing.Due to the spray printing provided in above-described embodiment
Two shower nozzles are needed to act on spraying successively in mode, so movable platform 2 needs to provide migration in the horizontal direction.When
So, because the method for spray printing is successively spray printing, so adjusting height, it is necessary to adjust the height of jet-printing head in manufacturing process
Process can be realized by vertical shift device.
On the basis of any one above-mentioned embodiment, the shower nozzle of syringe pump first and the shower nozzle of syringe pump second are arranged at
On syringe pump support, the two is fixed by syringe pump support.Optionally, the above-mentioned shower nozzle of syringe pump first and syringe pump
Two shower nozzles can be same injection device, and injection device has different storage cavitys, so as to realize the storage of different kind of material
Deposit.
Optionally, heater is high-pressure modular in above-described embodiment, or heater is high-pressure electrostatic field device 3.Need
It is noted that heater can use the form of high-tension electricity to heat material, firing rate is fast, and temperature-controllable.
Certainly, heater is not limited to the form of above-mentioned offer, can also select other kinds of heater in the prior art.
On the basis of any one above-mentioned embodiment, the inside diameter ranges of the injection needle of the shower nozzle of syringe pump first are
200 microns to 250 microns.
Optionally, internal diameter can be specially 232 μm, under the conditions of spray printing distance is 0.5 to 3mm near-field electrostatics, Ke Yizhi
Write out a diameter of 50 to 500nm fibre structure.
On the basis of any one above-mentioned embodiment, spray printing material can be artificial synthesized PLA high polymer materials.
Spray printing is carried out by the way that artificial synthesized PLA high polymer materials are heated into soldier, the higher polymer fiber of structure precision can be obtained
Structure.
From the perspective of product, the artificial synthesized PLA high polymer materials that the utility model uses are as melted material
Make cytoskeleton, it is not necessary to poisonous solvent is introduced, compared with traditional solution electrospinning process, for follow-up support
Cell culture is simultaneously implanted into the tissue of human body body or lesions position repair and has obvious advantage.
In a specific embodiment, above computer 1 connects movable platform 2 by motion control device 6, motion
Control device 6 includes the one or more in manipulator controller, retarder control device and electromechanical controlling device.
Optionally, due to using controlling organization of the computer 1 as movable platform 2, drive apparatus class therebetween
Type is not unique, and any one can receive computer 1 and control, and the mechanism for passing motion to movable platform 2 belongs to this
In the protection domain of utility model.
Optionally, above-mentioned motion control device 6 can be that Corvidae shellfish thinks motor.
Any one above-mentioned cytoskeleton preparation system in use, operating method specifically may comprise steps of：
Step S1, the spray printing material in the shower nozzle of heated syringe pump first.
Step S2, computer controls the shower nozzle of syringe pump first to the shaping position spray printing of collection device to form cell frame
Frame, computer controls movable platform movement, so that cell cytoskeleton is moved to the spray printing position of the shower nozzle of syringe pump second.
Step S3, computer controls the shower nozzle of syringe pump second to cell cytoskeleton spray printing biology ink；Computer control is movable
Platform moves, and shaping position is moved to the spray printing position of the shower nozzle of syringe pump first；Judge whether that spray printing is completed, if it has not, then
Return to step S2.
Spray printing material in step S1 in the shower nozzle of heated syringe pump first, the step of, it can specifically include：
Using the spray printing material in high-tension electricity device the first shower nozzle of heated syringe pump, spray printing material is artificial synthesized PLA
High polymer material.
The utility model has substantive distinguishing features following prominent and notable technological progress compared with prior art：
The melting electrostatic spinning wire shaping technology that the utility model introduces is used for the cell branch for being formed with suitable cell growth
Frame.Based on melting electrostatic spinning wire shaping technology be one kind can be formed with the case where computer system accurately controls a diameter of 50~
500nm polymer fiber.
Direct write electrostatic spinning technique is applied to the shaping of cytoskeleton by the utility model, and direct write electrostatic spinning technique can be with
The deposition path controllable precise of fiber is realized, the cytoskeleton that space is adapted to cell growth can be built.
The melted material that the utility model uses need not introduce poisonous solvent, with traditional solution electrospinning process
Compare, by the tissue of the compound implanting to human body or lesions position, as biomaterial is gradually degraded and inhaled in vivo
Receive, the cell of implantation is constantly bred in vivo and extracellular matrix secretion, corresponding tissue or organ is ultimately formed, so as to reach
Repair the purpose of wound and Reconstruction of The Function, and for follow-up support cell culture and be implanted into the tissue or organopathy of human body body
Damage position, which repair, has obvious advantage.
Each embodiment is described by the way of progressive in this specification, what each embodiment stressed be and other
The difference of embodiment, between each embodiment identical similar portion mutually referring to.
Cytoskeleton preparation system provided by the utility model is described in detail above.Tool used herein
Body example is set forth to principle of the present utility model and embodiment, and the explanation of above example is only intended to help and understood
Method and its core concept of the present utility model.It should be pointed out that for those skilled in the art, do not taking off
On the premise of from the utility model principle, some improvement and modification can also be carried out to the utility model, these are improved and modification
Also fall into the protection domain of the utility model claims.
Claims (6)
- A kind of 1. cytoskeleton preparation system, it is characterised in that including：For receiving the collection device of spray printing material；For the movable platform for driving collection device to move；For spraying spray printing material to form the shower nozzle of syringe pump first of cell cytoskeleton, the shower nozzle of syringe pump first is connected to Thermal source is provided to produce the heater of the spray printing material of molten condition and high-pressure electrostatic field device, the high-pressure electrostatic field device For making the spray printing material in the shower nozzle of syringe pump first under high temperature fused state spray to collection device；The high-pressure electrostatic Field device is connected with computer, and the shower nozzle of syringe pump first is arranged at the top of the collection device；For spraying the shower nozzle of syringe pump second of biology ink to the cell cytoskeleton, the shower nozzle of syringe pump second is arranged at described The top of collection device；Computer, for controlling the successively injection of the shower nozzle of syringe pump first and the syringe pump second shower nozzle, and for controlling Make movement of the movable platform relative to the shower nozzle of syringe pump first.
- 2. cytoskeleton preparation system according to claim 1, it is characterised in that the spray printing of the shower nozzle of syringe pump first Sophisticated with the distance between the collection plane of collection device scope is 0.5 to 3 millimeter.
- 3. cytoskeleton preparation system according to claim 2, it is characterised in that the movable platform is to move horizontally Platform, the horizontal shifting platform are also connected with vertical shift device, to carry out vertical shift after completing individual layer spray printing.
- 4. cytoskeleton preparation system according to claim 3, it is characterised in that the shower nozzle of syringe pump first and described The shower nozzle of syringe pump second is arranged on syringe pump support, and the heater is high-pressure modular, or the heater is institute State high-pressure electrostatic field device.
- 5. the cytoskeleton preparation system according to Claims 1-4 any one, it is characterised in that the syringe pump The inside diameter ranges of the injection needle of one shower nozzle are 200 microns to 250 microns.
- 6. cytoskeleton preparation system according to claim 5, it is characterised in that the spray printing material is artificial synthesized PLA high polymer materials；And/or the computer connects the movable platform by motion control device, the motion control device includes machine One or more in tool hand control device, retarder control device and electromechanical controlling device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621493047.7U CN206809488U (en) | 2016-12-30 | 2016-12-30 | A kind of cytoskeleton preparation system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201621493047.7U CN206809488U (en) | 2016-12-30 | 2016-12-30 | A kind of cytoskeleton preparation system |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106512105A (en) * | 2016-12-30 | 2017-03-22 | 广东工业大学 | Cell scaffold preparing system and method |
| CN109758613A (en) * | 2019-01-25 | 2019-05-17 | 上海交通大学医学院附属第九人民医院 | A kind of 3 D-printing combines biological three-dimensional printing technology to prepare tendon method |
| CN111317594A (en) * | 2020-02-28 | 2020-06-23 | 广州迈普再生医学科技股份有限公司 | Automatic artificial blood vessel production device |
-
2016
- 2016-12-30 CN CN201621493047.7U patent/CN206809488U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106512105A (en) * | 2016-12-30 | 2017-03-22 | 广东工业大学 | Cell scaffold preparing system and method |
| CN109758613A (en) * | 2019-01-25 | 2019-05-17 | 上海交通大学医学院附属第九人民医院 | A kind of 3 D-printing combines biological three-dimensional printing technology to prepare tendon method |
| CN111317594A (en) * | 2020-02-28 | 2020-06-23 | 广州迈普再生医学科技股份有限公司 | Automatic artificial blood vessel production device |
| CN111317594B (en) * | 2020-02-28 | 2023-10-20 | 广州迈普再生医学科技股份有限公司 | Automatic artificial blood vessel production device |
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20181218 Address after: 528000 Room 103, 71 Lihe Science and Technology Industry Center, 33 South Information Avenue, Shishan Town, Nanhai District, Foshan City, Guangdong Province Patentee after: FOSHAN QINGZI PRECISION MEASUREMENT AND CONTROL TECHNOLOGY CO., LTD. Address before: 510062 courtyard 729, Dongfeng East Road, Yuexiu District, Guangzhou, Guangdong Patentee before: Guangdong University of Technology |
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| TR01 | Transfer of patent right |