CN109730695A - Tissue fluid detection device - Google Patents
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- CN109730695A CN109730695A CN201811624488.XA CN201811624488A CN109730695A CN 109730695 A CN109730695 A CN 109730695A CN 201811624488 A CN201811624488 A CN 201811624488A CN 109730695 A CN109730695 A CN 109730695A
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Abstract
The present invention relates to a kind of tissue fluid detection devices, the tissue fluid detection device includes substrate and electrochemical sensor, the side of substrate is provided with for being pierced into the micropin for extracting tissue fluid in vivo, and micropin is provided with the duct for tissue fluid circulation, and duct extends through substrate from micropin tip is basad；Electrochemical sensor is set to the side that substrate deviates from micropin, and can with the interstitial fluid contacts that flow through micropin, to be detected to the tissue fluid.In use, on the skin by tissue fluid detection device patch, micropin is pierced into subcutaneously, and under capillarity, tissue fluid enters the duct of micropin, conveys along duct and contacted with electrochemical sensor, the physiologic information in tissue fluid is detected by electrochemical sensor.To keeping the extraction of tissue fluid integrated with the detection of physiologic information, method is simple, it can be achieved that the real-time detection of Human Physiology information and continuous detection, precision height, good reliability, and to the wound very little of human body, can fast implement clinical application.
Description
Technical field
The present invention relates to physiologic information detection technique fields, more particularly to tissue fluid detection device.
Background technique
Currently, include blood glucose, hormone, lactic acid isoconcentration acquisition, or such as antibiotic be present in human body drug it is dense
The acquisition of degree can play the role of the accurate treatment of disease vital.Such as: not curing diabetes thoroughly still now
Drug and method, so, blood sugar concentration is mainly clinically controlled by insulin injection or hypoglycemic medicine for oral administration.Its
In, the dosage needs of antidiabetic drugs adjust in due course according to human body level, and therefore, blood sugar test has become diabetes
Most important component part in nursing.
Now, there are mainly two types of the blood sugar detecting methods taken extensively in the world: (1) then venous blood samples liquid uses
Biochemical Analyzer centrifugal analysis, this method can more accurately obtain blood-sugar content, but operation is extremely complex, and process is slow
Slowly, it is only applicable to hospital's detection；(2) finger tip is taken a blood sample, and then detects blood glucose value, this method detection letter using quickly survey blood glucose meter
It is single, but due to cost and blood sampling frequency acceptance problem, it is limited that number is detected in general patient one day.Therefore, both
Detection method can not achieve the purpose to blood sugar for human body real-time detection, be unfavorable for accurately controlling blood sugar concentration by drug；And
And both methods can generate wound to human body.And due to the complexity of human body, at present still in the optical means of research and development, micro-
Wave detection method, impedance method and electrochemical method, although not having wound to human body, are still deposited when carrying out continuous blood sugar detection
It is remoter from practical application the problems such as signal-to-noise ratio is low, sensitivity is low, poor selectivity and error are big.
Summary of the invention
Based on this, it is necessary in view of the above-mentioned problems, providing a kind of tissue fluid detection device；The tissue fluid detection device can be real
Now to the continuous detection of tissue fluid, precision is high, good reliability, and to the wound very little of human body, can fast implement clinic and answer
With.
A kind of tissue fluid detection device, comprising:
Substrate, the side of the substrate are provided with for being pierced into the micropin for extracting tissue fluid in vivo, and the micropin is provided with
For the duct of tissue fluid circulation, the duct extends through the substrate from the micropin tip to the substrate；
Electrochemical sensor, the electrochemical sensor are set to the side that the substrate deviates from the micropin, and can
With the interstitial fluid contacts for flowing through the micropin, to be detected to the tissue fluid.
The length of the micropin is 100 μm~120 μm in one of the embodiments, and the diameter in the duct is 10 μm
~30 μm.
The quantity of the micropin is multiple in one of the embodiments, and multiple micropins form microneedle array.
The area of the microneedle array is 1mm in one of the embodiments,2~9mm2。
The micropin and the electrochemical sensor are to positive setting in one of the embodiments,.
The substrate is provided with the first groove in one of the embodiments, and the electrochemical sensor is set to described
In first groove.
The micropin and first groove are to positive setting in one of the embodiments, so that the micropin extracted
Tissue fluid is delivered in first groove by the duct.
Contact angle≤10 ° of the microneedle surface in one of the embodiments,；And/or
Contact angle≤10 ° of first groove surfaces.
The substrate is provided with the fluid channel of indent away from the side of the micropin in one of the embodiments, described
Fluid channel includes the second groove and third groove, and second groove is connected to the third groove by link slot, and described the
Two grooves and the micropin are to positive setting, so that by the duct to be delivered to described second recessed for the tissue fluid that extracts of the micropin
In slot, the electrochemical sensor is set in third groove.
Contact angle≤10 ° on the fluid channel surface in one of the embodiments,.
The electrochemical sensor includes working electrode, reference electrode and auxiliary electrode, institute in one of the embodiments,
Working electrode, the reference electrode and the auxiliary electrode is stated to be spaced apart from each other.
The tissue fluid detection device further includes cover board in one of the embodiments, the cover board and the substrate shape
At a cavity, the electrochemical sensor is set in the cavity.
The cavity has opening in one of the embodiments, so that the cavity is connected to outside.
The material of the substrate is high molecular material in one of the embodiments,；And/or
The material of the micropin is high molecular material；And/or
The material of the cover board is high molecular material.
The connection type between the substrate and the cover board is covalent bonding in one of the embodiments,.
Tissue fluid structure of the detecting device of the invention is simple, small in size, easy to carry.When in use, the present invention is organized
Liquid detection device is pasted on the skin, and micropin is pierced into subcutaneously, and under capillarity, tissue fluid enters the duct of micropin, along
Duct conveys and contacts with electrochemical sensor, is detected to tissue fluid by electrochemical sensor to obtain such as blood glucose, swash
The physiologic informations such as element, lactic acid, drug concentration.Especially, electrochemical sensor of the invention is the sensor of three-electrode system, especially
It is suitable for the concentration of glucose in detection tissue fluid, further according to concentration of glucose in tissue fluid and the concentration of glucose in blood
Relationship converts to obtain the concentration of glucose in blood.So the present invention make tissue fluid extraction and tissue fluid contained in ingredient connect
Continuous detection integration, method is simply, it can be achieved that the real-time detection of Human Physiology information.
Almost without feeling of pain when being pierced into skin due to the micropin that the present invention uses, so this minimally invasive physiologic information inspection
Survey method can overcome the defect of invasive detection method, realize that physiologic information continuously detects.Moreover, with noninvasive detection method
It compares, by micropin extracting interstitial fluid, then tissue fluid is detected, can be avoided physical efficiency bioelectricity for measuring signal
It influences, there is higher sensitivity, accuracy and reliability, there is important clinical application value and prospect.
Detailed description of the invention
Fig. 1 is the diagrammatic cross-section of the tissue fluid detection device of the embodiment of the present invention 1；
Fig. 2 is the structural schematic diagram of the microneedle array of the tissue fluid detection device of embodiment 1；
Fig. 3 is the electrochemical sensor schematic diagram of the tissue fluid detection device of embodiment 1；
Fig. 4 is diagrammatic cross-section of the tissue fluid detection device along link slot of the embodiment of the present invention 2；
Fig. 5 is the schematic diagram of the fluid channel of the tissue fluid detection device of embodiment 2.
In figure: 10, substrate；20, micropin；30, electrochemical sensor；40, cover board；101, the first groove, 102, it is second recessed
Slot；103, the second groove；104, link slot；201, duct；301, working electrode；302, reference electrode；303, auxiliary electrode.
Specific embodiment
Tissue fluid detection device provided by the invention will be described further below.
Embodiment 1:
As shown in Figure 1, the tissue fluid detection device of the present embodiment, for detecting human blood glucose concentration, hormone concentration, lactic acid
The physiologic informations such as concentration, drug concentration, so as to monitor the dosage of human body and to the metabolic degree of the drug, with
Realize the accurate treatment to disease.
The tissue fluid detection device includes substrate 10, and the side of the substrate 10 is provided with for being pierced into internal extraction group
The micropin 20 of liquid is knitted, the micropin 20 is provided with the duct 201 for tissue fluid circulation, and the duct 201 is sharp from the micropin 20
It holds to the substrate 10 and extends through the substrate 10；
Electrochemical sensor 30, the electrochemical sensor 30 are set to one that the substrate 10 deviates from the micropin 20
Side, and can with the interstitial fluid contacts that flow through the micropin 20, to be detected to the tissue fluid.
The present invention is by 20 extracting interstitial fluid of micropin, then in the side away from the micropin 20 of substrate 10 to tissue fluid
The physiologic informations such as concentration of glucose, lactic acid concn, hormone concentration, drug concentration detected, can be avoided physical efficiency bioelectricity
Influence for measuring signal has higher sensitivity, accuracy and reliability.
Specifically, the length of the micropin 20 is 100 μm~120 μm, the diameter in the duct 201 is 10 μm~30 μm.
So the wound generated to human body is very small, after the micron-sized micropin 20 is pierced into skin almost without feeling of pain.
In view of the tissue fluid that single micron-sized micropin 20 extracts is limited, it is preferred that the quantity of the micropin 20 is more
A, multiple micropins 20 form microneedle array as shown in Figure 2, area 1mm2~9mm2.So that tissue fluid detection device exists
The volume of the tissue fluid extracted simultaneously when use increases, and meets the requirement of detection.
It is appreciated that the quantity of micropin is unlimited in the microneedle array, and according to testing requirements, the body of the tissue fluid extracted
Product can satisfy detection demand.
It is appreciated that the electrochemical sensor 30 may be disposed at any position in the substrate 10.When electrochemistry passes
When sensor 30 is located on the extended line in duct 201, the electrochemical sensor 30 is with the micropin 20 to positive setting.Work as electrochemistry
When sensor 30 and duct 201 form certain displacement, the electrochemical sensor 30 is with the micropin 20 in offset setting.
Preferably, the micropin 20 is with the electrochemical sensor 30 to positive setting.To the tissue fluid that micropin 20 extracts
When being delivered to side of the substrate 10 away from micropin 20 through duct 201, it can directly be contacted with electrochemical sensor 30, detect tissue
The physiologic informations such as concentration of glucose, lactic acid concn, hormone concentration, drug concentration in liquid, the time is short, high-efficient, accuracy is good.
Specifically, the substrate 10 is provided with the first groove 101, it is recessed that the electrochemical sensor 30 is set to described first
In slot 101.To which the tissue fluid that the micropin 20 extracts is conveyed by the duct 201 and comes together in first groove 101
In, electrochemical sensor 30 can be made to obtain with tissue fluid and preferably contacted.
Again it is preferred to which the micropin 20 is arranged with first groove 101 to positive, so that the tissue that the micropin 20 extracts
Liquid is fed directly in first groove 101 by the duct 201, more efficient.
It is appreciated that when the quantity of micropin 20 is multiple and composition microneedle array, the area of first groove 101 >=
The area of the microneedle array, and make the microneedle array and first groove 101 to positive setting, so that the duct of micropin 20
201 are connected to the first groove 101, so that the tissue fluid for extracting micropin 20 directly comes together in the first groove 101.
Preferably, O can be used2, NO or NO2Corona treatment described in 20 surface of micropin, make its surface and liquid
Contact angle≤10 °.Preferably, which is water, to make 20 surface of micropin in Superhydrophilic, and then improves the extraction of tissue fluid
And transfer efficiency.
In view of O2Plasma processing efficiency highest, further, using O2Corona treatment described in 20 table of micropin
Face makes 10 ° of contact angle < of its surface and liquid.
Equally, O can be used2, NO or NO2Corona treatment described in 101 surface of the first groove, make described first recessed
Contact angle≤10 ° on slot 101 surface and liquid, it is preferred to use O2Corona treatment described in 101 surface of the first groove, make institute
10 ° of contact angle < for stating 101 surface of the first groove and liquid improve to make 101 surface of the first groove in Superhydrophilic
Transfer efficiency of the tissue fluid in the first groove 101.
The electrochemical sensor 30 is the sensor of three-electrode system, by reacting with ingredient tested in tissue fluid
And generate the electric signal directly proportional to the constituent concentration.
In conjunction with Fig. 3, the electrochemical sensor 30 includes working electrode 301, reference electrode 302 and auxiliary electrode 303, institute
It states working electrode 301, the reference electrode 302 and the auxiliary electrode 303 to be spaced apart from each other, and forms three-electrode system.
Wherein, the working electrode 301 includes one of non-enzyme electrode or enzyme electrode.
The enzyme electrode is in the carbon material or metal material surface immobilized enzyme.By introducing carbon material or metal
Material can increase the load capacity of enzyme, improve electron mobility in electrochemical reaction, enhance tissue fluid detection device sensitivity and
Detection accuracy.And the different types of enzyme fixed according to carbon material or metal material surface, it may be implemented to tissue fluid not
With the detection of information.Such as: when detection blood glucose, the enzyme that carbon material or metal material surface are fixed is glucose oxidase；Detection
When lactic acid, the enzyme that carbon material or metal material surface are fixed is lactic dehydrogenase.Equally, detect hormone when, carbon material or
The fixed enzyme of metal material surface is to the sensitive enzyme of the hormone response；When detecting antibiotic, carbon material or metal material table
The fixed enzyme in face is the corresponding enzyme of antibiotic.
The material of the non-enzyme electrode includes at least one of carbon material, metal material, metal oxide materials.It is described
Carbon material includes graphene, carbon nanotube etc., and the metal material includes gold, platinum, copper, tungsten etc., and the metal oxide includes
Copper oxide, cobalt sesquioxide, nickel oxide etc..It can be formed in by the method for 3D printing in the substrate 10.
Present invention is particularly suitable for the concentration of glucose in detection tissue fluid, further according to concentration of glucose in tissue fluid and blood
The relationship of concentration of glucose converts to obtain the concentration of glucose in blood in liquid.
Preferably, using the concentration of glucose in non-enzymatic electrode detection tissue fluid, the working electrode 301 is non-enzymatic electricity
Pole, including graphene composite layer, the graphene composite layer include 3D graphene and are carried on receiving in the 3D graphene
Rice material, contains metallic element in the nano material.
Wherein, 3D graphene is the graphene of three-dimensional structure, with the excellent chemical property of two-dimensional graphene and more greatly
Specific surface area, more nano materials can be loaded, and the network structure charge transfer resistance of 3D graphene is lower, electronics pass
Pass speed faster, thus, sensitivity and detectable limit of the working electrode 301 in glucose detection can be improved.
Meanwhile 3D graphene has the flexibility more more excellent than two-dimensional graphene, distortion, stretching of general level etc. become
Shape will not influence its property and characteristic, so that working electrode 301 be made to have flexibility.It in turn, can by the working electrode 301 satisfaction
The requirement of wearable tissue fluid detection device.
Wherein, the nano material includes at least one of metal, metal oxide, metallic compound, such as gold, platinum, copper,
Tungsten, copper oxide, cuprous oxide, cobalt sesquioxide, nickel oxide etc..The present invention is preferably metallic copper, cuprous oxide or copper oxide
At least one of.
Further, Cu2OPO4 +It can be realized the catalysis oxidation to glucose, then pass through detection electrochemical sensor electricity
The concentration of glucose, effect are preferable in stream detection tissue fluid.Simultaneously, it is contemplated that specific surface is low after cuprous oxide forms film, urges
Changing effect reduces.Therefore, the nano material is preferably cuprous oxide particle.
The reaction equation of the catalysis oxidation is as follows:
Cu2O+H2PO4 -→CuOPO4 ++2H++e-；
CuOPO4 ++ glucose → gluconolactone；
Gluconolactone → gluconic acid.
The reference electrode 302 is Ag/AgCl electrode, one layer can be printed in the substrate 10 by the method for 3D printing
Then metallic silver carries out chlorination to silver electrode using liquor ferri trichloridi and handles to obtain.
The auxiliary electrode 303 is that perhaps the inert electrodes such as platinum can pass through 3D printing or electrochemical deposition method system to gold
It is standby.
In application, the electrochemical sensor 30 can be realized as the detection to tissue fluid with interstitial fluid contacts, to obtain
The physiologic informations such as concentration of glucose, lactic acid concn, hormone concentration, drug concentration, easy to operate, fast response time, and have more
High detection sensitivity and detectable limit.
In view of tissue fluid detection device use environment and use when state, the tissue fluid detection device also wraps
Cover board 40 is included, the cover board 40 forms a cavity with the substrate 10, and the electrochemical sensor 30 is located in the cavity.From
And the tissue fluid that micropin 20 extracts will not be flowed out along the surface of substrate 10, it is particularly possible to guarantee that tissue fluid detection device is moving
Effect when being used under state.
It is appreciated that about 0.1mm~0.5mm will be electrochemical due to the cavity gap very little that cover board 40 and substrate 10 are formed
Learning when sensor 30 is set on cover board 40 can also come into full contact with tissue fluid.At this point, can also be by the electrochemical sensor 30
It is set on the cover board 40.
Preferably, the cavity has opening, so that the cavity is connected to outside.Thus with the evaporation of tissue fluid,
Micropin 20 can constantly extract intracorporal tissue fluid, to realize the purpose of real-time detection.
Specifically, the material of the substrate 10 can be high molecular material, the material of the micropin 20 can be macromolecule
The material of material, the cover board 40 may be high molecular material.
Preferably, when the material of the substrate 10, the micropin 20 and the cover board 40 is high molecular material, make entire
Tissue fluid detection device has flexibility, and bending and tensile property are good, can be worn on body surface, realize the reality of physiologic information
When detection and continuous detection.
Cause human body uncomfortable in view of the high molecular material of good biocompatibility can be avoided, it is preferred that the macromolecule
Material includes one of dimethyl silicone polymer (PDMS), polyurethane (PU), polyvinyl alcohol (PVA).
Preferably, the micropin 20 is an integral molding structure with the substrate 10, and preparation method includes micro- casting method, heat
One of mould pressing method.
Wherein, micro- casting method specifically includes:
(1) mold is provided, the mold includes underlying structure and microneedle configuration；
(2) high molecular material is cast in the mold, isolated integrally formed micropin 20 and substrate 10.
The hot moulding method specifically includes:
(1) mold is provided, the mold includes underlying structure and microneedle configuration；
(2) hot pressing of high molecular material, isolated integrally formed micropin 20 and substrate 10 are carried out with the mold.
It is appreciated that first groove 101 is integrated into substrate 10 when the substrate 10 is provided with the first groove 101
Type structure.
Preferably, the connection type between the substrate 10 and the cover board 40 is covalent bonding.That is, the substrate 10 with
The cover board 40 passes through the Covalent bonding together of its own high molecular material.
The application method of the tissue fluid detection device of the present embodiment, comprising the following steps:
S1 provides above-mentioned tissue fluid detection device；
The micropin 20 of the tissue fluid detection device is pierced into vivo by S2, under capillarity, described in tissue fluid entrance
The duct 201 of micropin 20 is simultaneously conveyed along the duct 201, contacts the tissue fluid with the electrochemical sensor 30；
S3 detects the tissue fluid by the electrochemical sensor 30.
Specifically, in step S2 on the skin by tissue fluid detection device patch, micropin 20 is pierced into subcutaneously, it is easy to use,
And there is no feeling of pain.
Specifically, can be directly obtained required after step S3 electrochemical sensor 30 detects the tissue fluid
Physiologic information or needs obtain required physiologic information after further converting.Such as: when detection blood glucose, electrochemical sensor 30
It detects in tissue fluid after the concentration of glucose, further according to the relationship of concentration of glucose in tissue fluid and the concentration of glucose in blood
Conversion obtains the concentration of glucose in blood.Method is simply, it can be achieved that the real-time detection of Human Physiology information.
Embodiment 2:
As shown in Figure 4 and Figure 5, on the basis of embodiment 1, the substrate 10 deviates from the one of the micropin 20 to the present embodiment
Side is provided with the fluid channel of indent, and the fluid channel includes the second groove 102 and third groove 103,102 He of the second groove
The third groove 103 is connected to by link slot 104, and second groove 102 is arranged with the micropin 20 to positive, so that described
The tissue fluid that micropin 20 extracts is delivered in second groove 102 by the duct 201, and the electrochemical sensor 30 is set
It is placed in third groove 103.To which the tissue fluid that micropin 20 extracts further comes together in third groove 103 through link slot 104
Afterwards, in third groove 103 using electrochemical sensor 30 to tissue fluid carry out concentration of glucose, lactic acid concn, hormone concentration,
The detection of the physiologic informations such as drug concentration keeps detection effect more preferable, more acurrate.
It is appreciated that at this time if when electrochemical sensor 30 is set on the cover board 40, the electrochemical sensing
Device 30 is located at the upper area of the third groove 103 so that electrochemical sensor 30 can be with the tissue in third groove 103
Liquid contact.
Preferably, the width of the link slot 104 is 0.2mm~1mm, the preferably circular groove of the third groove 103
And diameter is 2mm~5mm.The fluid channel is 10mm~15mm along the total length of the length extending direction of link slot 104.
Equally, O can be used2, NO or NO2Corona treatment fluid channel surface, make the fluid channel surface and liquid
Contact angle≤10 °, it is preferred to use O2Corona treatment described in fluid channel surface, make the fluid channel surface and liquid
10 ° of contact angle <, to make the fluid channel surface in Superhydrophilic, improve conveying effect of the tissue fluid in the fluid channel
Rate.
When in use, on the skin by tissue fluid detection device patch, micropin 20 is the tissue fluid detection device of the present embodiment
It is pierced into subcutaneously, under capillarity, tissue fluid enters the duct 201 of the micropin 20 and is delivered to along the duct 201 micro-
In second groove 102 of runner, then conveys and come together in the third groove 103 by link slot 104, in third groove
It contacts the tissue fluid with the electrochemical sensor 30, detects that concentration of glucose, the lactic acid in tissue fluid are dense
The physiologic informations such as degree, hormone concentration, drug concentration.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (15)
1. a kind of tissue fluid detection device characterized by comprising
Substrate, the side of the substrate are provided with for being pierced into the micropin for extracting tissue fluid in vivo, and the micropin is provided with for group
The logical duct of liquid stream is knitted, the duct extends through the substrate from the micropin tip to the substrate；
Electrochemical sensor, the electrochemical sensor are set to the side that the substrate deviates from the micropin, and can be with stream
Interstitial fluid contacts through the micropin, to be detected to the tissue fluid.
2. tissue fluid detection device according to claim 1, which is characterized in that the length of the micropin is 100 μm~120
μm, the diameter in the duct is 10 μm~30 μm.
3. tissue fluid detection device according to claim 1, which is characterized in that the quantity of the micropin be it is multiple, it is multiple
The micropin forms microneedle array.
4. tissue fluid detection device according to claim 3, which is characterized in that the area of the microneedle array is 1mm2~
9mm2。
5. tissue fluid detection device according to claim 1, which is characterized in that the micropin and the electrochemical sensor
To positive setting.
6. tissue fluid detection device according to claim 1, which is characterized in that the substrate is provided with the first groove, institute
Electrochemical sensor is stated to be set in first groove.
7. tissue fluid detection device according to claim 6, which is characterized in that the micropin and first groove are to just
Setting, so that the tissue fluid that the micropin extracts is delivered in first groove by the duct.
8. tissue fluid detection device according to claim 7, which is characterized in that contact angle≤10 ° of the microneedle surface；
And/or
Contact angle≤10 ° of first groove surfaces.
9. tissue fluid detection device according to claim 1, which is characterized in that the substrate deviates from the side of the micropin
It is provided with the fluid channel of indent, the fluid channel includes the second groove and third groove, and second groove and the third are recessed
Slot is connected to by link slot, and second groove and the micropin are to positive setting, so that the tissue fluid that the micropin extracts passes through
The duct is delivered in second groove, and the electrochemical sensor is set in third groove.
10. tissue fluid detection device according to claim 9, which is characterized in that the contact angle on the fluid channel surface≤
10°。
11. tissue fluid detection device according to claim 1, which is characterized in that the electrochemical sensor includes work
Electrode, reference electrode and auxiliary electrode, the working electrode, the reference electrode and the auxiliary electrode are spaced apart from each other.
12. described in any item tissue fluid detection devices according to claim 1~11, which is characterized in that the tissue fluid detection
Device further includes cover board, and the cover board and the substrate form a cavity, and the electrochemical sensor is set in the cavity.
13. tissue fluid detection device according to claim 12, which is characterized in that the cavity has opening, so that institute
Cavity is stated to be connected to outside.
14. tissue fluid detection device according to claim 12, which is characterized in that the material of the substrate is macromolecule material
Material；And/or
The material of the micropin is high molecular material；And/or
The material of the cover board is high molecular material.
15. tissue fluid detection device according to claim 12, which is characterized in that between the substrate and the cover board
Connection type is covalent bonding.
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| CN201811624488.XA CN109730695A (en) | 2018-12-28 | 2018-12-28 | Tissue fluid detection device |
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| CN201811624488.XA CN109730695A (en) | 2018-12-28 | 2018-12-28 | Tissue fluid detection device |
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| CN113974615A (en) * | 2021-11-18 | 2022-01-28 | 中山大学 | Tissue fluid detection device and system thereof |
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