CN109718396A - A kind of self-carry filler and preparation method thereof for shaping filling - Google Patents
A kind of self-carry filler and preparation method thereof for shaping filling Download PDFInfo
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- CN109718396A CN109718396A CN201910170080.8A CN201910170080A CN109718396A CN 109718396 A CN109718396 A CN 109718396A CN 201910170080 A CN201910170080 A CN 201910170080A CN 109718396 A CN109718396 A CN 109718396A
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Abstract
A kind of self-carry filler and preparation method thereof for shaping filling.Mainly solve the problems, such as be current shaping self-carry padding field there is the infiltration of self-carry filler and self-carry filler infiltration occur after frequently result in immunological rejection and influence user's life security.It is characterized by comprising the autologous fat cells of the extraction candidate stem cell of 20 ~ 30ml and 50 ~ 60ml.The advantage is that: the self-carry filler leakage scenarios generated in operative failure for beauty of human body type in terms of it is low with the injury in terms of life security.
Description
Technical field
The present invention relates to medical shaping fields, more particularly to a kind of self-carry filler for shaping filling and its preparation side
Method.
Background technique
With the improvement of people's living standards, in daily life, more and more women begin to focus on shaping and beauty
In terms of aspect, a large amount of new technologies and new tool are applied to shaping and beauty, but enter in market along with a large amount of capital, shaping beauty
There is such as technologies in appearance field it is lack of standardization, application technology development is unsmooth, technology is excessively old the problems such as.
Several operations application self-carry filler in shaping and beauty field existing at present includes the materials such as silica gel, hydrogel
There is autoimmunity repulsion in matter, there are the probability of operative failure after operation, pick up by oneself filler infiltration probability of happening 1% ~ 5%
Between, self-carry filler infiltration occur after, the body fluid circulatory etc. of human body is impacted, especially facial plasty cosmetically,
There is the case where prosthesis ruptures, deformation after the completion long period of performing the operation in the filler of selection, for face filling position, outside
It sees and loading position blood circulation nearby exerts a certain influence, when picking up by oneself filler material and contacting blood, exempt from
Epidemic disease rejection influences the life security of user, i.e., there are fillers after probability of failure and failure for existing Face filling plasty
Influence is caused for the life security of user.
Summary of the invention
The present invention is a kind of self-carry filler for shaping filling, candidate stem cell and 50 ~ 60ml including 20 ~ 30ml
Autologous fat cell.
Further, the autologous fat cell of the candidate stem cell including 23ml and 57ml.
A kind of preparation method of the self-carry filler for shaping includes the following steps:
1), haemocyte list adopts technology and obtains candidate stem cell, separation；
2), in vitro culture is carried out for obtaining candidate stem cell；
3) liposuction, is carried out, autologous fat cell is obtained；
4), the candidate stem cell of acquisition and autologous fat cell are mixed；
5), mixed cell extract is centrifuged, obtains the self-carry filler for shaping filling.
Further, the haemocyte list adopts technology as granulocyte stimulating factor stimulation, and blood cell separator divides after blood sampling
Be that 6%HES (hydroxyethyl starch) is added from, the separation condition, obtain upper liquid, the upper liquid at 4 DEG C, with 1500r/min
Under conditions of be centrifuged 10 minutes, sediment is washed 2 times with 1% albuminate aqueous.
Further, the condition of in vitro culture is that mononuclearcell presses 2*106Density is seeded in culture bottle, and culture is added
Base, at 37 DEG C, 5%CO2Under conditions of cultivate.
Further, the candidate stem cell and the operation of autologous fat mixing with cells are the candidate stem cell and from body fat
Fat cell carries out centrifugally operated in centrifuge, and centrifugal condition is the stirring 2h of 10 turns/min per minute.
Further, the cell molar density after the candidate stem cell progress in vitro culture of acquisition is 7*109A/L.
Further, the cell extract after the extraction is centrifuged, and the condition of centrifugation is 1000rpm.
Further, the self-carry filler molar density for shaping is 1*1010A/L.
Further, the body for face or chest moulds the fill in operation.
Specific embodiment
Embodiment 1
Self-blood is extracted in blood drawing, and centrifugation extracting blood obtains and extracts candidate stem cell, and liposuction obtains autologous fat cell, mentions
It takes candidate stem cell 20ml and autologous fat cell 60ml to be stirred 2h with 10 turns/min, is then by the molar density of extraction
7*109The candidate stem cell of a/L and the autologous fat cell of extracting are mixed, and are centrifuged under the centrifugal condition of 1000rpm
To the self-carry filler for shaping.
Embodiment 2
Self-blood is extracted in blood drawing, and centrifugation extracting blood obtains and extracts candidate stem cell, and liposuction obtains autologous fat cell, mentions
It takes candidate stem cell 23ml and autologous fat cell 57ml to be stirred 2h with 10 turns/min, is then by the molar density of extraction
7*109The candidate stem cell of a/L and the autologous fat cell of extracting are mixed, and are centrifuged under the centrifugal condition of 1000rpm
To the self-carry filler for shaping.
Embodiment 3
Self-blood is extracted in blood drawing, and centrifugation extracting blood obtains and extracts candidate stem cell, and liposuction obtains autologous fat cell, mentions
It takes candidate stem cell 25ml and autologous fat cell 55ml to be stirred 2h with 10 turns/min, is then by the molar density of extraction
7*109The candidate stem cell of a/L and the autologous fat cell of extracting are mixed, and are centrifuged under the centrifugal condition of 1000rpm
To the self-carry filler for shaping.
Embodiment 4
Self-blood is extracted in blood drawing, and centrifugation extracting blood obtains and extracts candidate stem cell, and liposuction obtains autologous fat cell, mentions
It takes candidate stem cell 30ml and autologous fat cell 50ml to be stirred 2h with 10 turns/min, is then by the molar density of extraction
7*109The candidate stem cell of a/L and the autologous fat cell of extracting are mixed, and are centrifuged under the centrifugal condition of 1000rpm
To the self-carry filler for shaping.
Cell molar density for each embodiment final product for the self-carry filler of shaping is respectively embodiment 1:
7.8*109A/L, embodiment 2:9.2*109A/L, embodiment 3:1*1010A/L, embodiment 4:8.5*109A/L
Cell molar density data according to the final product of each embodiment for the self-carry filler of shaping, described in embodiment 3
23ml extracts candidate stem cell and the latter made self-carry filler cell mole for shaping of 57ml autologous fat mixing with cells
Purpose is maximum, apery body breast portion eucaryotic cell structure needed for picking up by oneself filler similar in construction to transplanting, meets self-carry filling
The maximum of object transplanting requires: it is flexible, while picking up by oneself filler material and being extracted substantially from self, some materials are Hematopoietic Stem
Cell can greatly reduce the existing low immune response that does not have of self-carry filler material on the market or even without immune response
Technical characterstic.
Every technical staff's notice: of the invention although the present invention is described according to above-mentioned specific embodiment
Invention thought be not limited in the invention, any repacking with inventive concept will all be included in this patent protection of the patent right
In range.
Claims (10)
1. a kind of self-carry filler for shaping filling, it is characterised in that: candidate stem cell including 20 ~ 30ml and 50 ~
The autologous fat cell of 60ml.
2. a kind of self-carry filler for shaping filling according to claim 1, it is characterised in that: making including 23ml
The autologous fat cell of hemocytoblast and 57ml.
3. a kind of preparation method of the self-carry filler for shaping filling, characterized by the following steps:
1), haemocyte list adopts technology and obtains candidate stem cell, separation；
2), in vitro culture is carried out for obtaining candidate stem cell；
3) liposuction, is carried out, autologous fat cell is obtained；
4), the candidate stem cell of acquisition and autologous fat cell are mixed；
5), mixed cell extract is centrifuged, obtains the self-carry filler for shaping filling.
4. a kind of preparation method of self-carry filler for shaping filling according to claim 3, it is characterised in that: institute
It states haemocyte list and adopts technology as granulocyte stimulating factor stimulation, blood cell separator separates after blood sampling, and the separation condition is to add
Enter 6%HES (hydroxyethyl starch), obtain upper liquid, the upper liquid at 4 DEG C, with 1500r/min under conditions of be centrifuged 10 minutes,
Sediment is washed 2 times with 1% albuminate aqueous.
5. a kind of preparation method of self-carry filler for shaping filling according to claim 3, it is characterised in that:
The condition of in vitro culture is that mononuclearcell is seeded in culture bottle by 2*106 density, and culture medium, at 37 DEG C, 5%CO2's is added
Under the conditions of cultivate.
6. a kind of preparation method of self-carry filler for shaping filling according to claim 3, it is characterised in that: institute
State candidate stem cell and the operation of autologous fat mixing with cells be the candidate stem cell and autologous fat cell in centrifuge into
Row centrifugally operated, centrifugal condition are the stirring 2h of 10 turns/min per minute.
7. a kind of preparation method of self-carry filler for shaping filling according to claim 3, it is characterised in that: obtain
Cell molar density after the candidate stem cell progress in vitro culture obtained is 7*109/L.
8. a kind of preparation method of self-carry filler for shaping filling according to claim 3, it is characterised in that: institute
It states the cell extract after extracting to be centrifuged, the condition of centrifugation is 1000rpm.
9. a kind of preparation method of self-carry filler for shaping filling according to claim 3, it is characterised in that: institute
Stating for the self-carry filler molar density of shaping is 1*1010A/L.
10. a kind of self-carry filler for shaping filling described in any bar according to claim 1 ~ 9, which is characterized in that
Body for face or chest moulds the fill in operation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910170080.8A CN109718396A (en) | 2019-03-07 | 2019-03-07 | A kind of self-carry filler and preparation method thereof for shaping filling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910170080.8A CN109718396A (en) | 2019-03-07 | 2019-03-07 | A kind of self-carry filler and preparation method thereof for shaping filling |
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| CN109718396A true CN109718396A (en) | 2019-05-07 |
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| CN201910170080.8A Pending CN109718396A (en) | 2019-03-07 | 2019-03-07 | A kind of self-carry filler and preparation method thereof for shaping filling |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111714702A (en) * | 2020-07-02 | 2020-09-29 | 成都恩喜医疗管理有限公司 | Filler for shaping and shaping filling |
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| CN101534747A (en) * | 2006-07-10 | 2009-09-16 | 哥伦比亚大学 | De novo formation and regeneration of vascularized tissue from tissue progenitor cells and vascular progenitor cells |
| CN105420192A (en) * | 2015-10-12 | 2016-03-23 | 王泰华 | Method for separating and enriching hematopoietic stem cells in peripheral blood |
| CN106215240A (en) * | 2016-07-28 | 2016-12-14 | 广州赛莱拉干细胞科技股份有限公司 | adipose tissue composite preparation and preparation method and application thereof |
| CN106421920A (en) * | 2016-11-16 | 2017-02-22 | 沈阳细胞治疗工程技术研发中心有限公司 | Fat filler and preparation method thereof |
| CN106924815A (en) * | 2017-04-06 | 2017-07-07 | 上海俏佳人医疗美容门诊部股份有限公司 | For the autologous peripheral blood stem cells and autologous fat composition of shaping filling |
-
2019
- 2019-03-07 CN CN201910170080.8A patent/CN109718396A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101534747A (en) * | 2006-07-10 | 2009-09-16 | 哥伦比亚大学 | De novo formation and regeneration of vascularized tissue from tissue progenitor cells and vascular progenitor cells |
| CN105420192A (en) * | 2015-10-12 | 2016-03-23 | 王泰华 | Method for separating and enriching hematopoietic stem cells in peripheral blood |
| CN106215240A (en) * | 2016-07-28 | 2016-12-14 | 广州赛莱拉干细胞科技股份有限公司 | adipose tissue composite preparation and preparation method and application thereof |
| CN106421920A (en) * | 2016-11-16 | 2017-02-22 | 沈阳细胞治疗工程技术研发中心有限公司 | Fat filler and preparation method thereof |
| CN106924815A (en) * | 2017-04-06 | 2017-07-07 | 上海俏佳人医疗美容门诊部股份有限公司 | For the autologous peripheral blood stem cells and autologous fat composition of shaping filling |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111714702A (en) * | 2020-07-02 | 2020-09-29 | 成都恩喜医疗管理有限公司 | Filler for shaping and shaping filling |
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Application publication date: 20190507 |