CN111714702A - Filler for shaping and shaping filling - Google Patents
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- CN111714702A CN111714702A CN202010633428.5A CN202010633428A CN111714702A CN 111714702 A CN111714702 A CN 111714702A CN 202010633428 A CN202010633428 A CN 202010633428A CN 111714702 A CN111714702 A CN 111714702A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3834—Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3886—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0653—Adipocytes; Adipose tissue
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking
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Abstract
The invention discloses a filler for shaping, shaping and filling, which comprises hematopoietic stem cells, autologous fat cells and a biological material, and the filler comprises the following raw materials in parts by weight: 20-26 parts of hematopoietic stem cells, 54-60 parts of hematopoietic stem cells and the balance of biological materials, wherein the biological materials are swelled by using normal saline, the swelled biological materials are sequentially treated by using a human serum albumin solution, a glutaraldehyde solution and an ethanol solution, the mass volume concentration of the human serum albumin solution is set to be 0.3-0.7%, the mass concentration of the glutaraldehyde solution is 0.8-1.2%, the volume concentration of the ethanol solution is 80%, the treatment time of the human serum albumin solution is set to be 3 hours, the treatment time of the glutaraldehyde solution is set to be 30 minutes, and the treatment time of the ethanol solution is set to be 10 minutes. This a filler for moulding packing of plastic can reduce the rejection effect, passivates and disinfect, guarantees the result of use.
Description
Technical Field
The invention belongs to the technical field of filler processing, and particularly relates to a filler for shaping, shaping and filling.
Background
With the improvement of the living standard of people, more and more women begin to pay attention to the aspects of plastic cosmetology in daily life, and a great deal of new technologies and new means are applied to the aspects of plastic cosmetology, but along with the large amount of capital entering the market, the fields of plastic cosmetology have the problems of technical non-standardization, unsmooth development of applied technologies, over-old technology and the like. At present, several operation application self-extracting fillers in the field of plastic cosmetology comprise materials such as silica gel and hydrogel, autoimmune rejection exists, the probability of operation failure exists after the operation, and when the self-extracting filler material is contacted with blood, immunological rejection reaction occurs to influence the life safety of a user.
Therefore, in order to meet the current situation, the design and production of a filler for plastic filling are urgently needed to meet the requirements of practical use.
Disclosure of Invention
The present invention is directed to a filler for plastic filling, which solves the above problems of the prior art.
In order to achieve the purpose, the invention provides the following technical scheme: a filler for shaping and filling comprises hematopoietic stem cells, autologous fat cells and a biological material, wherein the raw materials comprise the following components in parts by weight: 20-26 parts of hematopoietic stem cells, 54-60 parts of hematopoietic stem cells and the balance of biological materials.
Preferably, the biological material is swelled with physiological saline, and the swelled biological material is sequentially treated with a human serum albumin solution, a glutaraldehyde solution and an ethanol solution.
Preferably, the mass volume concentration of the human serum albumin solution is set to be 0.3-0.7%, the mass concentration of the glutaraldehyde solution is 0.8-1.2%, and the volume concentration of the ethanol solution is 80%.
Preferably, the treatment time of the human serum albumin solution is set to 3 hours, the treatment time of the glutaraldehyde solution is set to 30 minutes, and the treatment time of the ethanol solution is set to 10 minutes.
A preparation method of a filler for plastic filling comprises the following steps:
s1, blood sampling: taking autologous blood, and obtaining hematopoietic stem cells from blood cells of the blood by using an apheresis technology;
s2, culturing: culturing the obtained hematopoietic stem cells in vitro to ensure the number of the hematopoietic stem cells;
s3, liposuction: performing autologous liposuction, then obtaining fat cells, then performing dedifferentiation treatment on the fat cells, and then culturing;
s4, centrifugal mixing: mixing and centrifuging the obtained hematopoietic stem cells and autologous fat cells by using a centrifuge to obtain a filler;
s5, filling: the filler is filled into the biomaterial using a dedicated device.
Preferably, the blood cell apheresis technology is granulocyte stimulating factor stimulation, and the blood cell is separated by a blood cell separator after blood collection, wherein the separation condition is that 6% HES (hydroxyethyl starch) is added to obtain supernatant, and the supernatant is centrifuged for 10 minutes at 4 ℃ and 1500 r/min.
Preferably, the dedifferentiated adipocytes are cultured to a confluency of 80%, the original medium is aspirated, the medium is cultured with 1640 medium without phenol red for 48 hours, and the filtrate is concentrated to a protein concentration of 1000 mg per liter to obtain a filler concentrated medium for use.
Preferably, the centrifuge for centrifugal mixing is set to TG18G of hochtian scientific instruments ltd of shanghai, and the time for centrifugation is set to 1 hour.
The invention has the technical effects and advantages that: the filler for shaping and filling, hematopoietic stem cells and autologous fat cells are used as filling, so that the influence of accidental rupture on a user can be reduced, and the rejection effect of the cells on the cells is poor; biomaterial utilizes human serum albumin to handle, can reduce the human rejection to biomaterial, utilizes glutaraldehyde and ethanol can passivate and disinfect biomaterial moreover, also can play the effect that reduces the rejection, and this a filler for plastic filling of plastic can reduce the rejection effect, passivates and disinfects, guarantees the result of use.
Detailed Description
The technical solutions in the present disclosure will be clearly and completely described below with reference to the present disclosure, and it is obvious that the described contents are only a part of the present disclosure, and not all of the present disclosure. All other matters which can be obtained by a person skilled in the art without making creative efforts based on the contents of the present invention belong to the protection scope of the present invention.
Example 1
The invention provides a filler for shaping, shaping and filling, which comprises hematopoietic stem cells, autologous fat cells and a biological material, wherein the filler comprises the following raw materials in parts by weight: 20 parts of hematopoietic stem cells and 54 parts of biological materials.
Specifically, the biological material is swelled by using normal saline, and the swelled biological material is sequentially treated by using a human serum albumin solution, a glutaraldehyde solution and an ethanol solution.
Specifically, the mass volume concentration of the human serum albumin solution is set to be 0.3%, the mass concentration of the glutaraldehyde solution is 0.8%, and the volume concentration of the ethanol solution is 80%.
Specifically, the treatment time of the human serum albumin solution is set to 3 hours, the treatment time of the glutaraldehyde solution is set to 30 minutes, and the treatment time of the ethanol solution is set to 10 minutes.
A preparation method of a filler for plastic filling comprises the following steps:
s1, blood sampling: taking autologous blood, and obtaining hematopoietic stem cells from blood cells of the blood by using an apheresis technology;
s2, culturing: culturing the obtained hematopoietic stem cells in vitro to ensure the number of the hematopoietic stem cells;
s3, liposuction: performing autologous liposuction, then obtaining fat cells, then performing dedifferentiation treatment on the fat cells, and then culturing;
s4, centrifugal mixing: mixing and centrifuging the obtained hematopoietic stem cells and autologous fat cells by using a centrifuge to obtain a filler;
s5, filling: the filler is filled into the biomaterial using a dedicated device.
Specifically, the blood cell apheresis technology is granulocyte stimulating factor stimulation, blood cells are separated by a blood cell separator after blood collection, the separation condition is that 6% HES (hydroxyethyl starch) is added to obtain supernatant, and the supernatant is centrifuged for 10 minutes at 4 ℃ under the condition of 1500 r/min.
Specifically, the dedifferentiated adipocytes were cultured to a confluency of 80%, the original medium was aspirated, the medium was cultured with 1640 medium without phenol red for 48 hours, and the filtrate was concentrated to a protein concentration of 1000 mg per liter to obtain a filler concentrated medium for use.
Specifically, the centrifuge for centrifugal mixing was set to TG18G of shanghai hertian scientific instruments ltd, and the time for centrifugation was set to 1 hour.
Example 2
The invention provides a filler for shaping, shaping and filling, which comprises hematopoietic stem cells, autologous fat cells and a biological material, wherein the filler comprises the following raw materials in parts by weight: 26 parts and 60 parts of hematopoietic stem cells, and the balance of biological materials.
Specifically, the biological material is swelled by using normal saline, and the swelled biological material is sequentially treated by using a human serum albumin solution, a glutaraldehyde solution and an ethanol solution.
Specifically, the mass volume concentration of the human serum albumin solution is set to be 0.7%, the mass concentration of the glutaraldehyde solution is 1.2%, and the volume concentration of the ethanol solution is 80%.
Specifically, the treatment time of the human serum albumin solution is set to 3 hours, the treatment time of the glutaraldehyde solution is set to 30 minutes, and the treatment time of the ethanol solution is set to 10 minutes.
A preparation method of a filler for plastic filling comprises the following steps:
s1, blood sampling: taking autologous blood, and obtaining hematopoietic stem cells from blood cells of the blood by using an apheresis technology;
s2, culturing: culturing the obtained hematopoietic stem cells in vitro to ensure the number of the hematopoietic stem cells;
s3, liposuction: performing autologous liposuction, then obtaining fat cells, then performing dedifferentiation treatment on the fat cells, and then culturing;
s4, centrifugal mixing: mixing and centrifuging the obtained hematopoietic stem cells and autologous fat cells by using a centrifuge to obtain a filler;
s5, filling: the filler is filled into the biomaterial using a dedicated device.
Specifically, the blood cell apheresis technology is granulocyte stimulating factor stimulation, blood cells are separated by a blood cell separator after blood collection, the separation condition is that 6% HES (hydroxyethyl starch) is added to obtain supernatant, and the supernatant is centrifuged for 10 minutes at 4 ℃ under the condition of 1500 r/min.
Specifically, the dedifferentiated adipocytes were cultured to a confluency of 80%, the original medium was aspirated, the medium was cultured with 1640 medium without phenol red for 48 hours, and the filtrate was concentrated to a protein concentration of 1000 mg per liter to obtain a filler concentrated medium for use.
Specifically, the centrifuge for centrifugal mixing was set to TG18G of shanghai hertian scientific instruments ltd, and the time for centrifugation was set to 1 hour.
Example 3
The invention provides a filler for shaping, shaping and filling, which comprises hematopoietic stem cells, autologous fat cells and a biological material, wherein the filler comprises the following raw materials in parts by weight: 23 parts of hematopoietic stem cells and 57 parts of biological materials.
Specifically, the biological material is swelled by using normal saline, and the swelled biological material is sequentially treated by using a human serum albumin solution, a glutaraldehyde solution and an ethanol solution.
Specifically, the mass volume concentration of the human serum albumin solution is set to be 0.5%, the mass concentration of the glutaraldehyde solution is 1%, and the volume concentration of the ethanol solution is 80%.
Specifically, the treatment time of the human serum albumin solution is set to 3 hours, the treatment time of the glutaraldehyde solution is set to 30 minutes, and the treatment time of the ethanol solution is set to 10 minutes.
A preparation method of a filler for plastic filling comprises the following steps:
s1, blood sampling: taking autologous blood, and obtaining hematopoietic stem cells from blood cells of the blood by using an apheresis technology;
s2, culturing: culturing the obtained hematopoietic stem cells in vitro to ensure the number of the hematopoietic stem cells;
s3, liposuction: performing autologous liposuction, then obtaining fat cells, then performing dedifferentiation treatment on the fat cells, and then culturing;
s4, centrifugal mixing: mixing and centrifuging the obtained hematopoietic stem cells and autologous fat cells by using a centrifuge to obtain a filler;
s5, filling: the filler is filled into the biomaterial using a dedicated device.
Specifically, the blood cell apheresis technology is granulocyte stimulating factor stimulation, blood cells are separated by a blood cell separator after blood collection, the separation condition is that 6% HES (hydroxyethyl starch) is added to obtain supernatant, and the supernatant is centrifuged for 10 minutes at 4 ℃ under the condition of 1500 r/min.
Specifically, the dedifferentiated adipocytes were cultured to a confluency of 80%, the original medium was aspirated, the medium was cultured with 1640 medium without phenol red for 48 hours, and the filtrate was concentrated to a protein concentration of 1000 mg per liter to obtain a filler concentrated medium for use.
Specifically, the centrifuge for centrifugal mixing was set to TG18G of shanghai hertian scientific instruments ltd, and the time for centrifugation was set to 1 hour.
Specifically, the filler for plastic filling compares the effects of the fillers of examples 1 to 3, and the filler of example 3 has the best effect, has a better ratio of hematopoietic stem cells to autologous fat cells than the other two groups, and has lower rejection compared with the other two groups due to the treatment time of the human serum albumin solution and the glutaraldehyde solution.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing description, it will be apparent to one skilled in the art that various changes, modifications, equivalents, and improvements may be made without departing from the spirit and scope of the invention.
Claims (8)
1. The filler for shaping and filling is characterized by comprising hematopoietic stem cells, autologous fat cells and a biological material, wherein the raw materials comprise the following components in parts by weight: 20-26 parts of hematopoietic stem cells, 54-60 parts of hematopoietic stem cells and the balance of biological materials.
2. A filling for plastic shaping filling according to claim 1, characterized in that: the biological material is swelled by using normal saline, and the swelled biological material is sequentially treated by using a human serum albumin solution, a glutaraldehyde solution and an ethanol solution.
3. A filling for plastic shaping filling according to claim 2, characterized in that: the human serum albumin solution is set to have a mass volume concentration of 0.3-0.7%, the glutaraldehyde solution is set to have a mass concentration of 0.8-1.2%, and the ethanol solution is set to have a volume concentration of 80%.
4. A filling for plastic shaping filling according to claim 2, characterized in that: the processing time of the human serum albumin solution was set to 3 hours, the processing time of the glutaraldehyde solution was set to 30 minutes, and the processing time of the ethanol solution was set to 10 minutes.
5. A preparation method of a filler for shaping and filling is characterized by comprising the following steps: the method comprises the following steps:
s1, blood sampling: taking autologous blood, and obtaining hematopoietic stem cells from blood cells of the blood by using an apheresis technology;
s2, culturing: culturing the obtained hematopoietic stem cells in vitro to ensure the number of the hematopoietic stem cells;
s3, liposuction: performing autologous liposuction, then obtaining fat cells, then performing dedifferentiation treatment on the fat cells, and then culturing;
s4, centrifugal mixing: mixing and centrifuging the obtained hematopoietic stem cells and autologous fat cells by using a centrifuge to obtain a filler;
s5, filling: the filler is filled into the biomaterial using a dedicated device.
6. The method for preparing a filler for plastic filling according to claim 5, wherein: the blood cell apheresis technology is granulocyte stimulating factor stimulation, blood cell separation is carried out by a blood cell separator after blood collection, the separation condition is that 6 percent HES (hydroxyethyl starch) is added to obtain supernatant, and the supernatant is centrifuged for 10 minutes at the temperature of 4 ℃ and the speed of 1500 r/min.
7. The method for preparing a filler for plastic filling according to claim 5, wherein: culturing the dedifferentiated adipocytes to a confluency of 80%, sucking out the original culture medium, culturing for 48 hours by using 1640 medium without phenol red, and concentrating the filtrate to a protein concentration of 1000 mg per liter to obtain a filler concentrated culture medium for later use.
8. The method for preparing a filler for plastic filling according to claim 5, wherein: the centrifuge for centrifugal mixing was set to TG18G of shanghai hertian scientific instruments ltd, and the time for centrifugation was set to 1 hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010633428.5A CN111714702A (en) | 2020-07-02 | 2020-07-02 | Filler for shaping and shaping filling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010633428.5A CN111714702A (en) | 2020-07-02 | 2020-07-02 | Filler for shaping and shaping filling |
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| CN111714702A true CN111714702A (en) | 2020-09-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202010633428.5A Pending CN111714702A (en) | 2020-07-02 | 2020-07-02 | Filler for shaping and shaping filling |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113713177A (en) * | 2021-10-15 | 2021-11-30 | 珠海美如初医疗美容有限公司 | Chest fat composite filler composition and preparation method and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1912109A (en) * | 2005-08-09 | 2007-02-14 | 中国人民解放军军事医学科学院野战输血研究所 | Structural method and application of tissue engineering adipose tissue |
| CN102625689A (en) * | 2009-05-04 | 2012-08-01 | 尼奥斯泰姆公司 | Method and composition for restoration of age-related tissue loss in the face or selected areas of the body |
| CN105749347A (en) * | 2016-02-24 | 2016-07-13 | 广州赛莱拉干细胞科技股份有限公司 | Cosmetic filler and preparation method thereof |
| CN109718396A (en) * | 2019-03-07 | 2019-05-07 | 秦文锋 | A kind of self-carry filler and preparation method thereof for shaping filling |
| KR20190070453A (en) * | 2017-12-13 | 2019-06-21 | 단국대학교 산학협력단 | Implant structure for plastic operation and method of manufacturing the same |
| CN110575564A (en) * | 2019-09-30 | 2019-12-17 | 张勇 | filler for medical plastic cosmetology and preparation method and application thereof |
-
2020
- 2020-07-02 CN CN202010633428.5A patent/CN111714702A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1912109A (en) * | 2005-08-09 | 2007-02-14 | 中国人民解放军军事医学科学院野战输血研究所 | Structural method and application of tissue engineering adipose tissue |
| CN102625689A (en) * | 2009-05-04 | 2012-08-01 | 尼奥斯泰姆公司 | Method and composition for restoration of age-related tissue loss in the face or selected areas of the body |
| CN105749347A (en) * | 2016-02-24 | 2016-07-13 | 广州赛莱拉干细胞科技股份有限公司 | Cosmetic filler and preparation method thereof |
| KR20190070453A (en) * | 2017-12-13 | 2019-06-21 | 단국대학교 산학협력단 | Implant structure for plastic operation and method of manufacturing the same |
| CN109718396A (en) * | 2019-03-07 | 2019-05-07 | 秦文锋 | A kind of self-carry filler and preparation method thereof for shaping filling |
| CN110575564A (en) * | 2019-09-30 | 2019-12-17 | 张勇 | filler for medical plastic cosmetology and preparation method and application thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113713177A (en) * | 2021-10-15 | 2021-11-30 | 珠海美如初医疗美容有限公司 | Chest fat composite filler composition and preparation method and application thereof |
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