CN109709234B - Method and device for detecting benzoic acid in seasoning - Google Patents
Method and device for detecting benzoic acid in seasoning Download PDFInfo
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- CN109709234B CN109709234B CN201910095647.XA CN201910095647A CN109709234B CN 109709234 B CN109709234 B CN 109709234B CN 201910095647 A CN201910095647 A CN 201910095647A CN 109709234 B CN109709234 B CN 109709234B
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Abstract
The invention relates to the technical field of food detection, and particularly discloses a method and a device for detecting benzoic acid in a seasoning. The detection device is used in the detection method, deionized water which is several times of the mass of the crushed seasoning is added into the crushing tank in batches, so that the sampling is convenient, and the sieve is arranged in the crushing tank, so that the particle size of the seasoning in the sample meets the standard, benzoic acid in solid matters (insoluble in water) in the seasoning is completely released, the accuracy of the detection result of the benzoic acid in the seasoning is improved, and the safety of the seasoning is ensured.
Description
Technical Field
The invention relates to the technical field of food detection, and particularly discloses a method and a device for detecting benzoic acid in a seasoning.
Background
The seasoning is a substance capable of harmonizing the color, smell and shape of food, changing the sensory property of the food and enhancing the special flavor. Seasoning can be classified into brewing seasoning, pickles seasoning, fresh vegetable seasoning, dried food seasoning, aquatic product seasoning and other seasoning according to the commodity property, wherein the pickles seasoning includes hot pickled mustard tuber, pickled ginger, pickled pepper and the like. At present, when the seasonings such as hot pickled mustard tuber, pickled pepper and the like are packaged, pickling liquid such as pickled pepper water is usually packaged and sold together to form a wet seasoning.
In general, in order to prolong the shelf life of seasonings or foods, additives having preservative function, such as benzoic acid, are added to the seasonings or foods. Benzoic acid, also known as benzoic acid, has the functions of sterilization, bacteriostasis and corrosion prevention, and is a common food additive. However, the consumption of excessive benzoic acid is harmful to human body and causes serious damage to liver and kidney, and thus, the content of benzoic acid in seasonings or foods should be strictly controlled. In the stipulation of the maximum using amount of benzoic acid in various foods, the maximum using amount of low-salt pickles, sauces and preserves is 0.5g/kg in the food additive use health standard (GB 2760-86).
At present, a plurality of methods for detecting benzoic acid mainly comprise thin layer chromatography, gas chromatography, liquid chromatography, ultraviolet spectrophotometry and the like. These methods require pretreatment of a sample to be tested, and among them, pretreatment of liquid seasonings such as soy sauce, vinegar, etc. is simple, and pretreatment of wet seasonings (including solid and liquid) is troublesome. The existing pretreatment mode of the wet seasoning is as follows: utilize rubbing crusher to smash wet flavouring, nevertheless at kibbling in-process, owing to have liquid, smash the thing and can splash to the inner wall on, difficult processing, and current rubbing crusher does not have the function of the crushing degree of control wet flavouring, probably leads to the crushing degree of wet flavouring not enough, and its inside benzoic acid can not release completely, leads to the testing result inaccurate.
Disclosure of Invention
The invention aims to provide a method and a device for detecting benzoic acid in a seasoning, and provides a new device and a new method for sample pretreatment for a wet seasoning, so that the crushing degree of the wet seasoning is ensured, and the accuracy of benzoic acid detection in the wet seasoning is improved.
In order to achieve the purpose, the basic scheme of the invention is as follows: benzoic acid detection device in flavouring, including smashing the jar, the motor is installed to the top of smashing the jar, the coaxial fixedly connected with pivot of output of motor, and the coaxial fixed mounting of pivot has crushing sword, vertical sliding connection has the screen cloth in smashing the jar, and the screen cloth separates smashing the jar for the upper tank body and lower jar body, crushing sword is located the upper tank internally, and the lateral wall fixedly connected with stopper of the upper tank body, screen cloth and stopper offset, and the first spring of one side fixedly connected with of stopper is kept away from to the screen cloth, and the one end of screen cloth and the diapire fixed connection of the lower jar body are kept away from to first spring.
The working principle and the beneficial effects of the basic scheme are as follows:
1. the screen mesh realizes the separation of solid and liquid in the wet seasoning, the solid is left in the upper tank body, and the liquid enters the lower tank body through the screen mesh. Drive crushing sword work through the motor drive pivot, smash the internal solid of jar, for the condition of smashing solid and liquid together, this basic scheme can reduce and smash thing degree of splashing.
2. After crushing, exert pressure to the screen cloth and make the screen cloth slide to jar body direction down, wash the pivot with deionized water, smash sword and go up jar body perisporium, make the crushing thing that is stained with to attach fall on the screen cloth, simultaneously, because the screen cloth slides to jar body direction down, the liquid level surpasss the screen cloth, crushing thing and liquid contact, the vibration screen cloth, in-process, crushing thing dispersion is in the liquid, the crushing thing that the particle diameter is less than the screen cloth sieve mesh gets into down jar internal, and the crushing thing that the particle diameter is greater than the screen cloth sieve mesh stays on the screen cloth, it continues to smash to wait after the screen cloth slides and resets, guarantee to thoroughly smash the solid in the wet flavouring with this, make benzoic acid in the solid completely release, improve the accuracy of testing result.
3. In this basic scheme, added the deionized water in to smashing the jar, firstly can wash pivot, crushing sword and go up jar body perisporium, secondly can dilute the wet flavouring, under the prerequisite that satisfies the detection concentration requirement, can get great volume during the sample, reduce the error, improve the accuracy of testing result.
Further, a cylinder body is fixedly installed in the lower tank body, one end of the cylinder body horizontally extends out of the lower tank body, a piston is connected in the cylinder body in a sliding mode and divides the cylinder body into a left cavity and a right cavity, the piston is hinged with a piston rod, an opening is formed in one side wall, far away from the piston, of the right cavity, and the piston rod penetrates through the opening; the left cavity is communicated with a water inlet pipe and a water outlet pipe, the water inlet pipe is provided with a first one-way control mechanism for guiding liquid into the left cavity, and the water outlet pipe is provided with a second one-way control mechanism for guiding the liquid out of the left cavity; the coaxial fixedly connected with driving gear of pivot, the driving gear meshing has reduction gear, the coaxial fixedly connected with disc of reduction gear, the edge fixedly connected with vertical axis of disc, the coaxial fixedly connected with bearing of vertical axis, the piston rod is kept away from the one end of piston and the outer lane fixed connection of bearing.
Coaxial fixed connection rotates at the epaxial driving gear of commentaries on classics, rotates with driving gear engaged's reduction gear to drive the disc and rotate, the disc rotates the pulling piston rod and removes, thereby control piston reciprocating sliding makes the volume constantly changing of left cavity: when the volume of the left cavity is increased, the internal pressure is reduced, and the liquid in the lower tank body enters the left cavity through the water inlet pipe; when the volume of the left cavity is reduced, the internal pressure is increased, and the liquid in the left cavity is discharged through the water outlet pipe, so that the liquid continuously enters the left cavity in the lower tank body, and the liquid continuously flows into the lower tank body from the left cavity, flows, so that the crushed materials and the liquid are uniformly mixed, and suspension is formed in the lower tank body, and the uniform sampling is ensured.
Furthermore, the first one-way control mechanism comprises a fixed plate and a sliding plate, the fixed plate is fixedly connected to the inner wall of the water inlet pipe, the sliding plate is slidably connected to the inner wall of the water inlet pipe, a limiting strip is fixedly connected to the inner wall of the water inlet pipe, the limiting strip is positioned on one side, away from the fixed plate, of the sliding plate, and the fixed plate is positioned between the sliding plate and the left cavity; the fixed plate is fixedly connected with a second spring, and one end of the second spring, which is far away from the fixed plate, is fixedly connected with the sliding plate; the fixed plate is provided with a through groove, the sliding plate is provided with a water opening, the water opening is connected with a plugging column for plugging the water opening in a sealing and sliding manner, the plugging column is fixedly connected with the fixed plate, and the plugging column is provided with a water groove axially arranged.
When the volume of the left cavity is increased, the internal pressure is reduced, and the sliding plate in the water inlet pipe slides towards the direction of the fixed plate, so that the sliding plate and the plugging column move relatively, and the liquid in the lower tank body flows into the left cavity through the water through groove on the plugging column and the through groove on the fixed plate successively; when the volume of the left cavity is reduced, the internal pressure is increased, the sliding plate in the water inlet pipe has the tendency of sliding in the direction away from the fixed plate, but the sliding plate is limited by the limiting strip at the moment, so that the sliding plate is static, the plugging column plugs the water through port, and the function of one-way water inlet of the water inlet pipe is realized.
Further, the second one-way control mechanism is the same as the first one-way control mechanism in terms of its components, and is different from the first one-way control mechanism in that the slide plate is located between the fixed plate and the left chamber.
When the volume of the left cavity is reduced, the internal pressure is increased, and at the moment, the plugging column in the water inlet pipe plugs the water through hole in the water inlet pipe, so that the sliding plate in the water outlet pipe slides towards the direction of the fixed plate, the sliding plate and the plugging column move relatively, and the liquid in the left cavity flows into the lower tank body through the water through groove on the plugging column and the through groove on the fixed plate successively; when the volume of the left cavity is increased, the internal pressure is reduced, the sliding plate in the water outlet pipe has the tendency of sliding in the direction away from the fixed plate, but the sliding of the sliding plate is limited by the limiting strip at the moment, so that the sliding plate is static, the plugging column plugs the water through port, and the function of one-way water outlet of the water outlet pipe is realized.
Further, the one end that left cavity was kept away from to inlet tube and outlet pipe all sets up towards the screen cloth.
The one end that left cavity was kept away from to inlet tube and outlet pipe all faces the screen cloth, consequently, no matter be the produced rivers power of rivers when inlet tube intake, still the rivers power that rivers produced when the outlet pipe goes out water all can act on the screen cloth for crushing thing on the screen cloth is at the effect of rivers power down with liquid misce bene, avoids the vibration screen cloth.
The benzoic acid detection method in the condiment that the benzoic acid detection device in the condiment is suitable for comprises:
s1: weighing Xg wet seasoning, putting the wet seasoning into the upper tank body, draining, and crushing;
s2: weighing N x Xg deionized water, wherein N is more than or equal to 1 and is an integer, adding the deionized water obtained in the step S2 into the crushing tank in batches, and the liquid level of the deionized water does not exceed the screen after each deionized water addition;
s3: after deionized water is added, controlling the screen to slide downwards to be immersed into liquid, mixing crushed materials on the screen with the liquid, then controlling the screen to slide upwards to separate the liquid, if the crushed materials are accumulated on the screen, continuing crushing, repeating the operation until the crushed materials are not accumulated on the screen when the screen slides away from the liquid, and uniformly mixing the crushed materials and the liquid in the lower tank to form suspension;
s4: taking 10g of the suspension sample in the step S3, adding 0.5mL of NaOH solution with the concentration of 1mol/L, mixing, adding deionized water and fixing the volume to 20 mL;
s5: uniformly mixing the solution with the constant volume in the step S4, placing the mixture into a centrifuge tube for centrifugation, and taking supernatant after centrifugation;
s6: adding ethyl acetate and n-hexane into the supernatant obtained in the step S5, stirring, standing, and separating an oil phase and a water phase;
s7: taking the water phase in the step S6 to perform high performance liquid chromatography measurement, wherein the parameters of the high performance liquid chromatography are set as follows:
a chromatographic column: a C18 column;
column temperature: room temperature;
mobile phase: 0.05% phosphoric acid aqueous solution and ethanol mixed solution, wherein the volume ratio of the 0.05% phosphoric acid aqueous solution to the ethanol is 100: 12;
detection wavelength: 224 nm;
sample introduction amount: 50 μ L.
The working principle and the beneficial effects of the detection method are as follows: in steps S2 and S3, deionized water is added to the grinding tank in batches, the rotating shaft, the grinding knife and the inner wall of the upper tank body can be cleaned, so that the attached ground material can fall on the screen, the screen is continuously immersed in the liquid, and the ground material with the particle size smaller than the screen hole on the screen can pass through the screen and enter the lower tank body, so that the solid in the wet seasoning is thoroughly ground, benzoic acid in the solid can be completely released, and the accuracy of the detection result is improved. In steps S4 to S6, NaOH solution is added so that NaOH reacts with benzoic acid to generate sodium benzoate which can be dissolved in water, the sample is centrifuged to settle the crushed material to obtain supernatant, and ethyl acetate and n-hexane are used to remove organic matters in the supernatant, thereby avoiding the organic matters from affecting the detection result of high performance liquid chromatography.
Further, in the step S2, when the deionized water is added in batches, the container for weighing the wet seasoning in the step S1 is rinsed with the deionized water, and the rinsing solution is added to the grinding tank.
Liquid or solid is remained in the container for weighing the wet seasoning, so that after the container is rinsed by deionized water, the rinsing liquid is added into the crushing tank, and the weighed wet seasoning can completely enter the crushing tank.
Further, in the step S4, before the constant volume is performed, the container for sampling is rinsed three times with deionized water, and the rinsing solution is used for the constant volume.
And (3) rinsing the container for sampling and using the rinsing liquid for constant volume so that 10g of samples are in the liquid after constant volume, and the accuracy of the detection result is improved.
Drawings
FIG. 1 is a schematic structural diagram of a benzoic acid detecting device in seasoning according to an embodiment of the present invention;
fig. 2 is an enlarged schematic view of a point a in fig. 1.
Detailed Description
The following is further detailed by way of specific embodiments:
reference numerals in the drawings of the specification include: the crushing tank comprises a crushing tank 1, an upper tank body 101, a lower tank body 102, a motor 2, a rotating shaft 3, a crushing cutter 4, a driving gear 5, a reduction gear 6, a disc 7, a vertical shaft 8, a bearing 9, a screen 10, a limiting block 11, a first spring 12, a cylinder body 13, a piston 131, a left chamber 132, a right chamber 133, a piston rod 134, a water inlet pipe 14, a water outlet pipe 15, a first one-way control mechanism 16, a first sliding plate 161, a first fixing plate 162, a first limiting strip 163, a second spring 164, a first through groove 165, a first blocking column 166, a first water inlet groove 167, a second one-way control mechanism 17, a second sliding plate 171, a second fixing plate 172, a second limiting strip 173, a third spring 174, a second through groove 175, a second blocking column 176 and a second water outlet groove 177.
The embodiment is basically as shown in figure 1: benzoic acid detection device in flavouring, including smashing jar 1, smash jar 1 top and install motor 2, motor 2's the coaxial fixedly connected with pivot 3 of output, 3 coaxial fixed mounting in pivot have crushing sword 4 and driving gear 5, and the driving gear 5 meshing has reduction gear 6, 6 coaxial fixedly connected with discs 7 of reduction gear, the fixedly connected with vertical axis 8 of edge of disc 7, the 8 coaxial fixedly connected with bearings of vertical axis 9.
Vertical sliding connection has screen cloth 10 in smashing jar 1, and screen cloth 10 will smash jar 1 and separate for last jar body 101 and lower jar body 102, smashes sword 4 and is located last jar body 101, goes up the lateral wall fixedly connected with stopper 11 of jar body 101, and screen cloth 10 offsets with stopper 11, and the first spring 12 of downside fixedly connected with of screen cloth 10, the lower extreme of first spring 12 and the diapire fixed connection of lower jar body 102.
A cylinder body 13 is fixedly arranged in the lower tank body 102, and the right end of the cylinder body 13 horizontally extends out of the lower tank body 102. The piston 131 is connected in the cylinder 13 in a sliding manner, the cylinder 13 is divided into a left chamber 132 and a right chamber 133 by the piston 131, the piston 131 is hinged to a piston rod 134, an opening is formed in the right side wall of the right chamber 133, the piston rod 134 penetrates through the opening, and the right end of the piston rod 134 is fixedly connected with the outer ring of the bearing 9.
The left chamber 132 is communicated with a water inlet pipe 14 and a water outlet pipe 15, and the left ends of the water inlet pipe 14 and the water outlet pipe 15 are both arranged upwards. The first one-way control mechanism 16 for guiding the liquid into the left chamber 132 is installed on the water inlet pipe 14, and the second one-way control mechanism 17 for guiding the liquid out of the left chamber 132 is installed on the water outlet pipe 15. Referring to fig. 2, the first unidirectional control mechanism 16 includes a first sliding plate 161 and a first fixing plate 162, the first fixing plate 162 is fixedly connected to the inner wall of the water inlet pipe 14, the first sliding plate 161 is slidably connected to the inner wall of the water inlet pipe 14, a first limit bar 163 is fixedly connected to the inner wall of the water inlet pipe 14, the first limit bar 163 is located on the left side of the first sliding plate 161, and the first fixing plate 162 is located between the first sliding plate 161 and the left chamber 132. A second spring 164 is fixedly connected between the first fixed plate 162 and the first sliding plate 161. First logical groove 165 has been seted up to first fixed plate 162, and first water service mouth has been seted up to first sliding plate 161, and first water service mouth sealing sliding connection has a first shutoff post 166 that is used for shutoff first water service mouth, and first shutoff post 166 and first fixed plate 162 fixed connection have seted up the first intake chamber 167 of axial setting on the first shutoff post 166.
The second one-way control mechanism 17 includes a second sliding plate 171 and a second fixed plate 172, the second fixed plate 172 is fixedly connected to the inner wall of the water outlet pipe 15, the second sliding plate 171 is slidably connected to the inner wall of the water outlet pipe 15, a second limiting bar 173 is fixedly connected to the inner wall of the water outlet pipe 15, the second limiting bar 173 is located on the right side of the second sliding plate 171, and the second sliding plate 171 is located between the second fixed plate 172 and the left chamber 132. A third spring 174 is fixedly connected between the second fixed plate 172 and the second sliding plate 171. The second fixing plate 172 is provided with a second through groove 175, the second sliding plate 171 is provided with a second water through opening, the second water through opening is connected with a second blocking column 176 used for blocking the second water through opening in a sealing and sliding manner, the second blocking column 176 is fixedly connected with the second fixing plate 172, and the second blocking column 176 is provided with a second water outlet groove 177 axially arranged.
The method for detecting benzoic acid in the seasoning comprises the following steps:
s1: weighing 10g of wet seasoning in a glass dish, putting the wet seasoning into the upper tank body 101, draining, and crushing;
s2: weighing 20g of deionized water, adding the deionized water obtained in the step S2 into the crushing tank 1 in batches, when adding the deionized water for the first three times, rinsing the glass ware obtained in the step S1 with the deionized water, adding seasonings adhered to the glass ware into the crushing tank 1, turning off the motor 2 when adding the deionized water every time, wherein the liquid level of the deionized water does not exceed the screen 10 after adding the deionized water every time;
s3: after deionized water is added, starting the motor 2 and controlling the screen mesh 10 to slide downwards to be immersed into liquid, mixing crushed materials on the screen mesh 10 with the liquid, controlling the screen mesh 10 to slide upwards to separate from the liquid after 20 seconds, if the crushed materials stacked on the screen mesh 10 exist, continuing to crush, repeating the operation until the crushed materials stacked on the screen mesh 10 do not exist when the screen mesh 10 slides upwards to separate from the liquid, and uniformly mixing the crushed materials and the liquid in the lower tank body to form suspension;
s4: taking 10g of the suspension sample in the step S3, adding 0.5mL of NaOH solution with the concentration of 1mol/L, mixing, adding deionized water and fixing the volume to 20 mL;
s5: uniformly mixing the solution with the constant volume in the step S4, placing the solution into a centrifuge tube for centrifugation, centrifuging the solution for one minute under the condition of 10000r/min, and taking supernatant after centrifugation;
s6: adding 10mL of ethyl acetate and 10mL of n-hexane into the supernatant obtained in the step S5, stirring, standing, and separating an oil phase and a water phase;
s7: taking the water phase in the step S6 to perform high performance liquid chromatography measurement, wherein the parameters of the high performance liquid chromatography are set as follows:
a chromatographic column: a C18 column,
Column temperature: at room temperature,
Mobile phase: 0.05% phosphoric acid aqueous solution and ethanol mixed solution, wherein the volume ratio of the 0.05% phosphoric acid aqueous solution to the ethanol is 100:12, and the detection wavelength is as follows: 224nm of,
Sample introduction amount: 50 μ L.
In steps S1 to S3, after the wet seasoning is placed in the upper tank 101, the solid in the wet seasoning is located on the screen 10, and the liquid in the wet seasoning drops into the lower tank 102 through the screen 10, so as to realize the solid-liquid separation of the wet seasoning. The motor 2 is started, the motor 2 drives the rotating shaft 3 to rotate, the rotating shaft 3 drives the crushing knife 4 and the driving gear 5 to rotate, and the rotating crushing knife 4 crushes the solid on the screen mesh 10 to change the solid into crushed objects. Meanwhile, the driving gear 5 is engaged with the reduction gear 6, the reduction gear 6 rotates to drive the disc 7 to rotate, the vertical shaft 8 fixedly connected to the edge of the disc 7 rotates to drive the right end of the piston rod 134 to rotate, and the piston 131 is driven to slide left and right in a reciprocating manner, so that the volume of the left chamber 132 is changed constantly.
When the volume of the left chamber 132 increases, the internal pressure decreases, and at this time, in the water outlet pipe 15, because the second limiting bar 173 is arranged on the right side of the second sliding plate 171 to limit the position of the second limiting bar, the second sliding plate 171 is still, and the second blocking column 176 blocks the second water through opening. In the water inlet pipe 14, the first sliding plate 161 slides rightward, the first blocking post 166 moves leftward relative to the first sliding plate 161, and the left end of the first water inlet groove 167 of the first blocking post 166 is exposed out of the first sliding plate 161, so that the liquid in the lower tank 102 flows into the left chamber 132 through the first water inlet groove 167 and the first through groove 165 successively.
When the volume of the left chamber 132 decreases, the internal pressure increases, and at this time, in the water inlet pipe 14, the first sliding plate 161 slides and returns to the left by the second spring 164, and because the left side of the first sliding plate 161 has the first stopper 163, the first sliding plate 161 is stationary after returning, and the first blocking column 166 blocks the first water passage port. In the water outlet pipe 15, the second sliding plate 171 slides leftwards, the second blocking column 176 moves rightwards relative to the second sliding plate 171, and the right end of the second water outlet groove 177 on the second blocking column 176 is exposed out of the second sliding plate 171, so that the liquid in the left chamber 132 flows into the lower tank 102 through the second water discharge groove and the second through groove 175 successively.
Therefore, with the change of the volume of the left chamber 132, the water inlet pipe 14 continuously feeds water, the water outlet pipe 15 continuously feeds water, the liquid in the lower tank 102 continuously flows, and the crushed material and the liquid entering the lower tank 102 after being screened by the screen 10 are uniformly mixed. In addition, because the left ends of the water inlet pipe 14 and the water outlet pipe 15 are both arranged upwards, water flow formed when the water inlet pipe 14 absorbs water and the water outlet pipe 15 discharges water passes through the screen 10, crushed objects accumulated on the screen 10 are scattered, the crushed objects with the particle diameter smaller than that of the screen 10 can smoothly pass through the screen 10, and the screen 10 is prevented from being blocked.
After no more crushed material is deposited on the screen 10, the sampling is performed in step S4, and the motor 2 is turned off after the sampling, and then the steps S5 to S7 are performed.
Through the device and the method, the solid in the wet seasoning can be thoroughly crushed, so that the benzoic acid in the solid is completely released, the accuracy of the detection result is improved, and the lowest concentration of the benzoic acid in the seasoning can be detected to be 5 ng/mL.
The foregoing is merely an example of the present invention and common general knowledge of known specific structures and features of the embodiments is not described herein in any greater detail. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several changes and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the present invention.
Claims (5)
1. Benzoic acid detection device in flavouring, including smashing the jar, the motor is installed to the top of smashing the jar, the coaxial fixedly connected with pivot of output of motor, and the coaxial fixed mounting of pivot has crushing sword, its characterized in that: the crushing tank is vertically and slidably connected with a screen, the screen divides the crushing tank into an upper tank body and a lower tank body, the crushing cutter is positioned in the upper tank body, the side wall of the upper tank body is fixedly connected with a limiting block, the screen is abutted against the limiting block, one side of the screen, far away from the limiting block, is fixedly connected with a first spring, and one end of the first spring, far away from the screen, is fixedly connected with the bottom wall of the lower tank body; a cylinder body is fixedly installed in the lower tank body, one end of the cylinder body horizontally extends out of the lower tank body, a piston is connected in the cylinder body in a sliding mode, the cylinder body is divided into a left cavity and a right cavity by the piston, the piston is hinged to a piston rod, an opening is formed in one side wall, away from the piston, of the right cavity, and the piston rod penetrates through the opening; the left cavity is communicated with a water inlet pipe and a water outlet pipe, one ends of the water inlet pipe and the water outlet pipe, which are far away from the left cavity, are both arranged towards the screen, a first one-way control mechanism for guiding liquid into the left cavity is arranged on the water inlet pipe, and a second one-way control mechanism for guiding the liquid out of the left cavity is arranged on the water outlet pipe; the rotating shaft is coaxially and fixedly connected with a driving gear, the driving gear is meshed with a reduction gear, the reduction gear is coaxially and fixedly connected with a disc, the edge of the disc is fixedly connected with a vertical shaft, the vertical shaft is coaxially and fixedly connected with a bearing, and one end of a piston rod, far away from the piston, is fixedly connected with an outer ring of the bearing; the first one-way control mechanism comprises a fixed plate and a sliding plate, the fixed plate is fixedly connected to the inner wall of the water inlet pipe, the sliding plate is slidably connected to the inner wall of the water inlet pipe, a limiting strip is fixedly connected to the inner wall of the water inlet pipe, the limiting strip is located on one side, away from the fixed plate, of the sliding plate, and the fixed plate is located between the sliding plate and the left cavity; the fixed plate is fixedly connected with a second spring, and one end of the second spring, which is far away from the fixed plate, is fixedly connected with the sliding plate; the fixed plate is provided with a through groove, the sliding plate is provided with a water opening, the water opening is connected with a plugging column for plugging the water opening in a sealing and sliding manner, the plugging column is fixedly connected with the fixed plate, and the plugging column is provided with a water groove axially arranged.
2. The apparatus for detecting benzoic acid in seasoning, as claimed in claim 1, wherein: the second one-way control mechanism is the same as the first one-way control mechanism in terms of its components, and differs from the first one-way control mechanism in that the sliding plate is located between the fixed plate and the left chamber.
3. The method for detecting benzoic acid in the seasoning is characterized by comprising the following steps: the apparatus for detecting benzoic acid in seasoning according to claim 1, comprising the steps of:
s1: weighing Xg wet seasoning, putting the wet seasoning into an upper tank body, draining, and crushing;
s2: weighing N x Xg deionized water, wherein N is more than or equal to 1 and is an integer, adding the deionized water obtained in the step S2 into a grinding tank in batches, and the liquid level of the deionized water does not exceed a screen after each addition;
s3: after deionized water is added, controlling the screen to slide downwards to be immersed into liquid, mixing crushed materials on the screen with the liquid, then controlling the screen to slide upwards to separate the liquid, if the crushed materials stacked on the screen exist, continuing crushing until the crushed materials stacked on the screen do not exist when the screen slides upwards to separate the liquid, and uniformly mixing the crushed materials in the lower tank body with the liquid to form suspension;
s4: taking 10g of the suspension sample in the step S3, adding 0.5mL of NaOH solution with the concentration of 1mol/L, mixing, adding deionized water and fixing the volume to 20 mL;
s5: uniformly mixing the solution with the constant volume in the step S4, placing the mixture into a centrifuge tube for centrifugation, and taking supernatant after centrifugation;
s6: adding ethyl acetate and n-hexane into the supernatant obtained in the step S5, stirring, standing, and separating an oil phase and a water phase;
s7: taking the water phase in the step S6 to perform high performance liquid chromatography measurement, wherein the parameters of the high performance liquid chromatography are set as follows:
a chromatographic column: a C18 column;
column temperature: room temperature;
mobile phase: 0.05% phosphoric acid aqueous solution and ethanol mixed solution, wherein the volume ratio of the 0.05% phosphoric acid aqueous solution to the ethanol is 100: 12;
detection wavelength: 224 nm;
sample introduction amount: 50 μ L.
4. The method for detecting benzoic acid in a seasoning, as defined in claim 3, wherein: in the step S2, when deionized water is added in batches, the container for weighing the wet seasoning in the step S1 is rinsed with deionized water, and the rinsing solution is added to the grinding tank.
5. The method for detecting benzoic acid in a seasoning, as defined in claim 4, wherein: in the step S4, before the volume is fixed, the container for sampling is rinsed three times with deionized water, and the rinsing solution is used for fixing the volume.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104807939A (en) * | 2015-05-11 | 2015-07-29 | 梧州市产品质量检验所 | Method for detecting benzoic acid in food |
| CN107875887A (en) * | 2017-10-13 | 2018-04-06 | 百奥森（江苏）食品安全科技有限公司 | A kind of block food secure sample detection separates out preparation facilities with solution |
| CN207786691U (en) * | 2017-10-20 | 2018-08-31 | 重庆生搏饲料有限公司 | A kind of feed processing grinding device |
| CN108722561A (en) * | 2018-05-09 | 2018-11-02 | 芜湖拓云农业技术有限公司 | A kind of crush maize device for agricultural production |
| CN109046643A (en) * | 2018-09-20 | 2018-12-21 | 李影 | A kind of aniseed processing efficient pulverizing device |
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| CN103084250A (en) * | 2012-12-29 | 2013-05-08 | 苏州韩博厨房电器科技有限公司 | Kitchen waste processor with cleaning agent adding device |
| CN205839894U (en) * | 2016-07-07 | 2016-12-28 | 高树选 | A kind of domestic kitchen garbage grinder structure |
| CN206578122U (en) * | 2016-12-27 | 2017-10-24 | 天津市恒润环境工程有限公司 | A kind of refuse classification and processing unit |
| CN108773101A (en) * | 2018-06-13 | 2018-11-09 | 广州市中潭空气净化科技有限公司 | A kind of garbage recovery device of the environmental protection in municipal works field |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104807939A (en) * | 2015-05-11 | 2015-07-29 | 梧州市产品质量检验所 | Method for detecting benzoic acid in food |
| CN107875887A (en) * | 2017-10-13 | 2018-04-06 | 百奥森（江苏）食品安全科技有限公司 | A kind of block food secure sample detection separates out preparation facilities with solution |
| CN207786691U (en) * | 2017-10-20 | 2018-08-31 | 重庆生搏饲料有限公司 | A kind of feed processing grinding device |
| CN108722561A (en) * | 2018-05-09 | 2018-11-02 | 芜湖拓云农业技术有限公司 | A kind of crush maize device for agricultural production |
| CN109046643A (en) * | 2018-09-20 | 2018-12-21 | 李影 | A kind of aniseed processing efficient pulverizing device |
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