CN109700785A - A kind of Rubus parvifolius Cellulose/Chitosan composite membrane for treating 2 grades of diabetes - Google Patents
A kind of Rubus parvifolius Cellulose/Chitosan composite membrane for treating 2 grades of diabetes Download PDFInfo
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- CN109700785A CN109700785A CN201811371629.1A CN201811371629A CN109700785A CN 109700785 A CN109700785 A CN 109700785A CN 201811371629 A CN201811371629 A CN 201811371629A CN 109700785 A CN109700785 A CN 109700785A
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Abstract
The present invention provides a kind of Rubus parvifolius Cellulose/Chitosan composite membranes and preparation method thereof, for treating the diabetes that extent is 2 grades (Wanger staging), the composite membrane is the preparation method comprises the following steps: preparation Rubus parvifolius (Rubus parvifolius L.) cellulose；Then 1- ethyl-3-methylimidazole acetate (Emim Ac) ion liquid dissolving Rubus parvifolius cellulose is used, tape casting on glass plate is poured on, is prepared into hygrometric state Rubus parvifolius cellulose membrane；After elution ionic liquid, hygrometric state Rubus parvifolius cellulose membrane is put into chitosan acetic acid solution and is impregnated, surface moisture is air-dried at room temperature, 60 DEG C of drying in baking oven is subsequently placed in and takes out, up to Rubus parvifolius Cellulose/Chitosan composite membrane, facing the used time is cut into the shape to fit with affected part.The composite membrane use in Rubus parvifolius L and chitosan as raw material, there is good bacteriostasis and biological degradability, safe and reliable, environmental protection, without side-effects is used for a long time.
Description
Technical field
The present invention relates to medical instruments fields, and in particular to a kind of Rubus parvifolius Cellulose/Chitosan with bacteriostasis is multiple
Film and preparation method thereof is closed, can be used for the treatment of 2 grades of diabetes.
Background technique
Diabetes are the complication of diabetes, diabetic concurrent various different degrees of vascular lesions and neuropathy
Becoming different leads to the illnesss such as lower limb infection, ulcer.According to Wanger staging, diabetes one are divided into 5 grades, including 0 grade, 1 grade, 2
Grade, 3 grades, 4, grade and 5 grades.2 grades of patient with diabetic feet foots start ulcer occur, and are often accompanied by infection, and infection site can be deposited
In a variety of germs.
Rubus parvifolius (Rubus parvifolius L.) is rose family rubus plant, shrub, and root, stem, Ye Jieke are used as medicine,
Have effects that clearing heat and cooling blood, eliminating stagnation to stop pain；Its bacteriostatic experiment the result shows that, Rubus parvifolius is to staphylococcus aureus, green pus bar
The various bacterias such as bacterium have inhibiting effect, and external application can control eczema, dermatitis, have huge potentiality in terms of antibacterial therapy.
Cellulose is natural polymer, and rich content in nature has good biocompatibility and biological degradability,
And intensity it is high, it is renewable but do not have antibiotic property；Chitosan is the derivative of natural biological polysaccharide chitin, has antibacterial, disappears
Scorching effect, safe and non-toxic, biocompatibility and biological degradability are excellent, but membrane material is made in chitosan, and intensity is low, crisp
Property is big.
With the development of technology of biological membrane, prepares cellulose chitosan complex film and make cellulose and chitosan respective scarce
Point is compensated for.Using Rubus parvifolius cellulose and chitosan as raw material, researching and developing, there is the composite membrane of bacteriostasis to be used for 2 grades of diabetes
The treatment of foot can effectively reduce the risk of patient with diabetic feet foot infection, improve the quality of living, and cellulose chitosan is multiple
Closing film, there is biological degradability to be had no toxic side effect using safety and environmental protection.
In consideration of it, special propose the invention.
Summary of the invention
It is prepared the purpose of the present invention is to provide a kind of Rubus parvifolius Cellulose/Chitosan composite membrane and preparation method thereof
Composite membrane has biological degradability, and fungistatic effect is good, can be used for treating 2 grades of diabetes.
Rubus parvifolius Cellulose/Chitosan composite membrane is achieved through the following technical solutions:
(1) preparation of Rubus parvifolius cellulose；
(2) preparation of hygrometric state Rubus parvifolius cellulose membrane；
(3) Rubus parvifolius cellulose is mixed with chitosan, is prepared into composite membrane.
Further, the Rubus parvifolius cellulose is prepared by the following method to obtain: taking Rubus parvifolius raw material and 4%-7%HNO3It presses
1: the solid-liquid ratio of (8-15) is put into boiling reflux 3h in the reaction vessel of reflux unit, then reactor content is washed into
Property, 2%-4%Na OH boiling reflux 2h is added, is washed out to neutrality, products therefrom 35%H2O2Bleaching 3h, filtering
Fall H2O2Solution, products therefrom are washed to neutrality, and it is spare that powder is made in 60 DEG C of dryings.
Further, the hygrometric state Rubus parvifolius cellulose membrane is prepared by the following method to obtain: Rubus parvifolius cellulose powder is weighed,
It is added into Emim Ac ionic liquid, then ultrasonic wave added dissolution pours them onto tape casting on glass plate, puts after cooling
Enter in 50% ethanol/water solution and stand 1.5h, ionic liquid is precipitated, 3-5 times repeatedly, after elution ionic liquid, by cellulose
Film takes out, and hygrometric state Rubus parvifolius cellulose membrane is just made in natural air drying surface moisture.
Further, the Rubus parvifolius Cellulose/Chitosan composite membrane, is prepared by the following method to obtain: by hygrometric state Rubus parvifolius
Cellulose membrane, which is put into chitosan acetic acid solution, impregnates 2h, take out air-dry 2h be placed in baking oven 60 DEG C of drying to get.
Further, the Rubus parvifolius Cellulose/Chitosan composite membrane, it is characterised in that: Rubus parvifolius raw material includes Chinese medicine thatch
Dry root, stem or the herb of the certain kind of berries.
Further, the Rubus parvifolius Cellulose/Chitosan composite membrane preparation process, it is characterised in that: according to weighing body
The adding proportion of product ratio, Rubus parvifolius cellulose and Emim Ac ionic liquid is 1: 20.
Further, the Rubus parvifolius Cellulose/Chitosan composite membrane preparation process, it is characterised in that: the ultrasound
Assist course of dissolution, power 100-150W, time 15-25min.
Further, the Rubus parvifolius Cellulose/Chitosan composite membrane, it is characterised in that: the chitosan acetic acid is molten
Liquid concentration is 3%-7%.
Beneficial effects of the present invention:
Rubus parvifolius Cellulose/Chitosan composite membrane of the present invention, with rose family rubus plant Rubus parvifolius and chitosan
It is environmentally protective as raw material, it is degradable, there is good fungistatic effect, it is easy to use, safe for treating 2 grades of diabetes
Reliably.
Detailed description of the invention
Fig. 1 is the production technological process of composite membrane of the present invention.
Fig. 2 is one embodiment of the invention staphylococcus aureus bacteriostatic experiment result figure.
Specific embodiment
To keep purpose, the technical solution of the embodiment of the present invention clearer, the present invention will be made below further detailed
Description.But this should not be interpreted as during the scope of the above subject matter of the present invention is limited to the following embodiments, it is all based on the present invention
The technology that content is realized all belongs to the scope of the present invention.
Embodiment 1
The present invention provides a kind of Rubus parvifolius Cellulose/Chitosan composite membranes for treating 2 grades of diabetes, including prepare as follows
Step:
(1) preparation of Rubus parvifolius cellulose；
(2) preparation of hygrometric state Rubus parvifolius cellulose membrane；
(3) Rubus parvifolius cellulose is mixed with chitosan, is prepared into composite membrane.
The Rubus parvifolius cellulose is prepared by the following method to obtain: take Rubus parvifolius raw material and 5%HNO3 by 1: 15 solid-liquid ratio
It is put into boiling reflux 3h in the reaction vessel of reflux unit, then reactor content is washed to neutrality, 2%Na OH is added and boils
Boiling reflux 2h, is washed out to neutrality.Products therefrom 35%H2O2 bleaching 3h, filters out H2O2 solution, products therefrom
It is spare that powder is made in 60 DEG C of dryings to neutrality in washing.
The hygrometric state Rubus parvifolius cellulose membrane is prepared by the following method to obtain: weighing Rubus parvifolius cellulose powder 5g, is added extremely
In 100mL Emim Ac ionic liquid, 120W ultrasonic wave added dissolves 25min, then pours them onto tape casting on glass plate, to
It is put into after cooling in 50% ethanol/water solution and stands 1.5h, ionic liquid is precipitated, it 5 times repeatedly, will after elution ionic liquid
Cellulose membrane takes out, natural air drying surface moisture.
Hygrometric state Rubus parvifolius cellulose membrane is put into 5% chitosan acetic acid solution and impregnates 2h, air-dried 2h is taken out and is placed on baking oven
In 60 DEG C drying to get the Rubus parvifolius Cellulose/Chitosan composite membrane.
Embodiment 2
The present invention provides a kind of Rubus parvifolius Cellulose/Chitosan composite membranes for treating 2 grades of diabetes, including prepare as follows
Step:
(1) preparation of Rubus parvifolius cellulose；
(2) preparation of hygrometric state Rubus parvifolius cellulose membrane；
(3) Rubus parvifolius cellulose is mixed with chitosan, is prepared into composite membrane.
The Rubus parvifolius cellulose is prepared by the following method to obtain: take Rubus parvifolius raw material and 6%HNO3 by 1: 12 solid-liquid ratio
It is put into boiling reflux 3h in the reaction vessel of reflux unit, then reactor content is washed to neutrality, 3%Na OH is added and boils
Boiling reflux 2h, is washed out to neutrality.Products therefrom 35%H2O2 bleaching 3h, filters out H2O2 solution, products therefrom
It is spare that powder is made in 60 DEG C of dryings to neutrality in washing.
The hygrometric state Rubus parvifolius cellulose membrane is prepared by the following method to obtain: weighing Rubus parvifolius cellulose powder 10g, is added extremely
In 200mL Emim Ac ionic liquid, 130W ultrasonic wave added dissolves 20min, then pours them onto tape casting on glass plate, to
It is put into after cooling in 50% ethanol/water solution and stands 1.5h, ionic liquid is precipitated, it 4 times repeatedly, will after elution ionic liquid
Cellulose membrane takes out, natural air drying surface moisture.
Hygrometric state Rubus parvifolius cellulose membrane is put into 6% chitosan acetic acid solution and impregnates 2h, air-dried 2h is taken out and is placed on baking oven
In 60 DEG C drying to get the Rubus parvifolius Cellulose/Chitosan composite membrane.
Embodiment 3
The present invention provides a kind of Rubus parvifolius Cellulose/Chitosan composite membranes for treating 2 grades of diabetes, including prepare as follows
Step:
(1) preparation of Rubus parvifolius cellulose；
(2) preparation of hygrometric state Rubus parvifolius cellulose membrane；
(3) Rubus parvifolius cellulose is mixed with chitosan, is prepared into composite membrane.
The Rubus parvifolius cellulose is prepared by the following method to obtain: Rubus parvifolius raw material and 7%HNO3 being taken to put by 1: 8 solid-liquid ratio
Enter boiling reflux 3h in the reaction vessel of reflux unit, then reactor content is washed to neutrality, 4%Na OH is added and boils
Flow back 2h, is washed out to neutrality.Products therefrom 35%H2O2 bleaching 3h, filters out H2O2 solution, products therefrom is washed
It washs into, it is spare that powder is made in 60 DEG C of dryings.
The hygrometric state Rubus parvifolius cellulose membrane is prepared by the following method to obtain: weighing Rubus parvifolius cellulose powder 10g, is added extremely
In 200mL Emim Ac ionic liquid, 150W ultrasonic wave added dissolves 15min, then pours them onto tape casting on glass plate, to
It is put into after cooling in 50% ethanol/water solution and stands 1.5h, ionic liquid is precipitated, it 3 times repeatedly, will after elution ionic liquid
Cellulose membrane takes out, natural air drying surface moisture.
Hygrometric state Rubus parvifolius cellulose membrane is put into 7% chitosan acetic acid solution and impregnates 2h, air-dried 2h is taken out and is placed on baking oven
In 60 DEG C drying to get the Rubus parvifolius Cellulose/Chitosan composite membrane.
In order to illustrate beneficial effects of the present invention, present invention applicant prepare according to the method for embodiment 1 Rubus parvifolius cellulose/
Chitosan complex film carries out following experiments:
Rubus parvifolius Cellulose/Chitosan composite membrane is padded on conical flask bottom, staphylococcus aureus culture solution is added, then
It is cultivated by 3% inoculum concentration inoculation staphylococcus aureus.Blank group: staphylococcus aureus is added with model conical flask
Culture solution is inoculated with staphylococcus aureus；Two groups respectively at culture 0,4,8,12,16,20, for 24 hours sampling survey OD value, production life
Long curve.Referring to FIG. 2, when in staphylococcus aureus culture medium there are when Rubus parvifolius Cellulose/Chitosan composite membrane, it is golden yellow
Staphylococcic growth is obviously inhibited.
Claims (9)
1. it is a kind of treat 2 grades of diabetes Rubus parvifolius Cellulose/Chitosan composite membrane, which is characterized in that preparation method include with
Lower step:
(1) preparation of Rubus parvifolius cellulose；
(2) preparation of hygrometric state Rubus parvifolius cellulose membrane；
(3) Rubus parvifolius cellulose is mixed with chitosan, is prepared into composite membrane.
2. the compound membrane preparation method of Rubus parvifolius Cellulose/Chitosan according to claim 1, which is characterized in that the thatch
Certain kind of berries cellulose the preparation method comprises the following steps: taking Rubus parvifolius raw material and 4%-7%HNO3By 1: the solid-liquid ratio of (8-15) is put into reflux unit
Boiling reflux 3h in reaction vessel, then reactor content is washed to neutrality, 2%-4%Na OH boiling reflux 2h is added, so
After wash to neutrality, products therefrom 35%H2O2Bleaching 3h, filters out H2O2Solution, products therefrom are washed to neutrality, in
It is spare that powder is made in 60 DEG C of dryings.
3. the compound membrane preparation method of Rubus parvifolius Cellulose/Chitosan according to claim 1, which is characterized in that described is wet
State Rubus parvifolius cellulose membrane the preparation method comprises the following steps: weigh Rubus parvifolius cellulose powder, be added into Emim Ac ionic liquid, ultrasound is auxiliary
Hydrotropy solution, then pours them onto tape casting on glass plate, is put into 50% ethanol/water solution after cooling and stands 1.5h, made
Ionic liquid is precipitated, and 3-5 times repeatedly, then elution ionic liquid takes out cellulose membrane, and natural air drying surface moisture is just made
At hygrometric state Rubus parvifolius cellulose membrane.
4. the compound membrane preparation method of Rubus parvifolius Cellulose/Chitosan according to claim 1, which is characterized in that the thatch
The method that certain kind of berries cellulose and chitosan are prepared by mixing into composite membrane are as follows: hygrometric state Rubus parvifolius cellulose membrane is put into chitosan acetic acid solution
Middle immersion 2h, the air-dried 2h of taking-up, which is placed in baking oven, dries to get Rubus parvifolius Cellulose/Chitosan composite membrane for 60 DEG C.
5. the compound membrane preparation method of Rubus parvifolius Cellulose/Chitosan according to claim 2, which is characterized in that the thatch
Certain kind of berries raw material includes: dry root, stem or the herb of middle Rubus parvifolius L.
6. the compound membrane preparation method of Rubus parvifolius Cellulose/Chitosan according to claim 3, which is characterized in that according to weight
The adding proportion of volume ratio, Rubus parvifolius cellulose and Emim Ac ionic liquid is 1: 20.
7. the compound membrane preparation method of Rubus parvifolius Cellulose/Chitosan according to claim 3, which is characterized in that described is super
Sound auxiliary dissolution, power 100-150W, time 15-25min.
8. the compound membrane preparation method of Rubus parvifolius Cellulose/Chitosan according to claim 4, which is characterized in that the shell
Glycan acetic acid solution concentration is 3%-7%.
9. a kind of Rubus parvifolius Cellulose/Chitosan composite membrane prepared such as any one of claim 1-8.
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| CN201811371629.1A CN109700785A (en) | 2018-11-12 | 2018-11-12 | A kind of Rubus parvifolius Cellulose/Chitosan composite membrane for treating 2 grades of diabetes |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111777692A (en) * | 2020-08-17 | 2020-10-16 | 南京赛尔健生物技术有限公司 | Rubus parvifolius graded polysaccharide, preparation method and application in depression resistance |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2314856B (en) * | 1996-07-03 | 2000-04-12 | British Textile & Technology G | Cellulose product |
| CN101733002A (en) * | 2009-12-31 | 2010-06-16 | 厦门大学 | PH-sensitive type cation regenerated cellulose composite film as well as preparation method and application thereof |
| CN105778159A (en) * | 2016-05-17 | 2016-07-20 | 天津工业大学 | Method for preparing chitosan/cellulose blended membrane |
-
2018
- 2018-11-12 CN CN201811371629.1A patent/CN109700785A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2314856B (en) * | 1996-07-03 | 2000-04-12 | British Textile & Technology G | Cellulose product |
| CN101733002A (en) * | 2009-12-31 | 2010-06-16 | 厦门大学 | PH-sensitive type cation regenerated cellulose composite film as well as preparation method and application thereof |
| CN105778159A (en) * | 2016-05-17 | 2016-07-20 | 天津工业大学 | Method for preparing chitosan/cellulose blended membrane |
Non-Patent Citations (4)
| Title |
|---|
| VINITA DUBEY. ET AL: "Pervaporative separation of ethanolwater azeotrope using a novel chitosan-impregnated bacterial cellulose membrane and chitosan–poly(vinyl alcohol) blends", 《JOURNAL OF MEMBRANE SCIENCE》 * |
| 周德生，谭元生: "《简明中药手册》", 31 January 2014, 山西科学技术出版社 * |
| 张晓艳: "离子液体中再生纤维素膜的制备及性能研究", 《广西大学硕士学位论文》 * |
| 高峰: "《药用高分子材料学》", 31 October 2014, 华东理工大学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111777692A (en) * | 2020-08-17 | 2020-10-16 | 南京赛尔健生物技术有限公司 | Rubus parvifolius graded polysaccharide, preparation method and application in depression resistance |
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