CN109700691A - A kind of microneedle patch of compensating water and removing wrinkle and preparation method thereof - Google Patents
A kind of microneedle patch of compensating water and removing wrinkle and preparation method thereof Download PDFInfo
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- CN109700691A CN109700691A CN201910166231.2A CN201910166231A CN109700691A CN 109700691 A CN109700691 A CN 109700691A CN 201910166231 A CN201910166231 A CN 201910166231A CN 109700691 A CN109700691 A CN 109700691A
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- hyaluronic acid
- microneedle
- microneedle patch
- active peptide
- moisturizing
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- NGWKGSCSHDHHAJ-YPFQVHCOSA-N Liquoric acid Chemical compound C1C[C@H](O)C(C)(C)C2CC[C@@]3(C)[C@]4(C)C[C@H]5O[C@@H]([C@](C6)(C)C(O)=O)C[C@@]5(C)[C@@H]6C4=CC(=O)C3[C@]21C NGWKGSCSHDHHAJ-YPFQVHCOSA-N 0.000 description 1
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- KIUKXJAPPMFGSW-MNSSHETKSA-N hyaluronan Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)C1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H](C(O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-MNSSHETKSA-N 0.000 description 1
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Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Cosmetics (AREA)
- Materials For Medical Uses (AREA)
Abstract
本发明涉及一种补水祛皱的微针贴片及其制备方法，涉及护肤品领域。所述微针贴片包括底座和微针，多个针头均匀分布在底座上，底座和针头是由主体支撑成分构成，功效成分分散在主体支撑成分中；主体支撑成分为透明质酸，功效成分为活性肽P‑WH。本发明的微针贴片可有效穿透皮肤角质层被表皮层和真皮上层的组织间液溶解，释放P‑WH到表皮及真皮组织并进行扩散，刺激皮肤细胞胶原蛋白分泌，对皮肤老化、粗糙进行预防及修复，起到补水抗衰老的作用。
The invention relates to a microneedle patch for moisturizing and removing wrinkles and a preparation method thereof, and relates to the field of skin care products. The microneedle patch includes a base and microneedles, a plurality of needles are evenly distributed on the base, the base and the needles are composed of a main body support component, and the functional components are dispersed in the main body support component; the main body support component is hyaluronic acid, and the functional components is the active peptide P-WH. The microneedle patch of the present invention can effectively penetrate the stratum corneum of the skin and be dissolved by the interstitial fluid of the epidermis and the upper dermis, release P-WH to the epidermis and dermis for diffusion, stimulate the secretion of collagen in skin cells, and reduce the effects of skin aging, Rough for prevention and repair, play the role of hydration and anti-aging.
Description
Technical field
The invention belongs to skin care fields, are related to a kind of microneedle patch and preparation method thereof of compensating water and removing wrinkle.
Background technique
Before the mankind can trace back to several centuries for beautiful pursuit and on the demand for reversing aging bring to influence,
And with the growth in man-year age, it is necessarily accompanied with irreversible physiological ageing phenomenon, the most common facial tissue that shows as
Loose and wrinkle generation.Some skin-protection products are added to vitamin, can prevent and improve dry skin aging phenomenon, such as with
Vitamin B3 existing for niacinamide, niacin ester-formin can be used as the precursor of the endogenous enzyme confactor with anti-oxidation function,
It is used in skin care item product, but niacinamide and nicotinate, as cosmetic formulations, the niacin of hydrolysis also can under low dosage
Induce strong red swelling of the skin；It is thin can to reduce sunburn after uv irradiation for vitamin E equally as typical antioxidant
It in born of the same parents and free radical, and is used as moisturizer, activity can extend in conjunction with vitamin C；It is known that vitamin C is one
Kind antioxidant, but its acidity is too strong, can damage keratoderma.The major defect of most of compensating water and removing wrinkle class skin care item is
Effect is slow and inaccurate, also easily causes the skin problems such as allergy.On the other hand, it due to the obstruction of keratoderma, only applies
The skin care item for being distributed in skin surface are difficult really to be absorbed, and cosmetic result is also had a greatly reduced quality.
Summary of the invention
To solve the above problems, the present invention provides a kind of microneedle patch and preparation method thereof of compensating water and removing wrinkle.
The technical solution adopted by the present invention are as follows:
A kind of microneedle patch of compensating water and removing wrinkle, including pedestal and micropin, multiple syringe needles are evenly distributed on pedestal, pedestal and
Syringe needle is made of body supports ingredient, and functional component is dispersed in body supports ingredient；The body supports ingredient is
Bright matter is sour (Hyaluronic acid, HA), and functional component is active peptide P-WH.
The microneedle patch area 4cm2-10000cm2；A length of 100 μm -3000 μm of the micropin needle, microneedle substrate
100 μm -1000 μm of diameter；600 μm -10000 μm of the micropin spacing, the microneedle array 1-200 needle.It is preferred that microneedle patch
Area 4cm2-6cm2；A length of 200 μm -500 μm of micropin needle, 200 μm -500 μm of microneedle substrate diameter；1700 μm of micropin spacing-
2000 μm, 140 needle of microneedle array.
The syringe needle is pyramid, cone or class cone, preferably class cone.
The hyaluronan molecule amount is 10-400kDa, preferably 10kDa, 100kDa, 400kDa.
A kind of preparation method of the microneedle patch of compensating water and removing wrinkle, including the following steps:
1) hyaluronic acid is dissolved in ultrapure water, the hyaluronic acid solution that compound concentration is 2%~10%, after mixing evenly
2h or more is stood, 4 DEG C of refrigerations is placed in, stands overnight, so that hyaluronic acid water suction is sufficiently expanded to gel, obtains hyaluronic acid
Gel.
2) active peptide P-WH is dissolved in ultrapure water, compound concentration is the active peptide solution of 100-800 μ g/ml.
3) the active peptide solution in step 2) and the hyaluronic acid derivatives in step 1) is mixed according to volume ratio 1:1~1:8
It closes, stands 2h or more after mixing evenly, be placed in 4 DEG C of refrigerations, stand overnight, mixed liquor water suction is made sufficiently to be expanded to gel.
4) at room temperature, mixed liquor gelatinous in step 3) is cast on the die cavity of microneedles template, keeps mixed liquor complete
All standing die cavity is vacuum-treated 10-30min.
5) microneedles template being placed in ventilation natural air drying, curing and demolding is dry, the microneedle patch of compensating water and removing wrinkle is obtained,
It is saved at 4 DEG C after sealing.
Active peptide P-WH is a kind of nonapeptide molecule that relative molecular weight is 941.2, and there is stimulation skin collagen to secrete,
Prevent skin, coarse activity of fighting against senium, it has therefore proved that its almost no cytotoxicity and hemolytic, while reacting and sending out without sensitization
It is raw, and can be stable for up in serum 24 hours, there is outstanding development prospect.
Beneficial effects of the present invention: microneedle patch prepared by the present invention is made using hyaluronic acid and active peptide P-WH, thoroughly
Bright matter acid is natural moisturizing factor, is to constitute one of extracellular matrix and the main component of cytoplasm, is intercellular filling
Object plays an important role for the form, structure, function of skin；Active peptide used has stimulation Skin Cell collagen
Secretion, to skin aging, the coarse effect for being prevented and being repaired, almost no cytotoxicity and hemolytic, no class sensitization are anti-
It answers.Hyaluronic acid is mixed with active peptide, skin effect is repaired in conjunction with moisturizing moisturizing and anti-aging, is opened with outstanding application
Hair prospect.
Detailed description of the invention
Fig. 1 is molding microneedle patch schematic diagram；
Fig. 2 is the preparation flow figure of microneedle patch；
Fig. 3 (a) and Fig. 3 (b) is partial enlarged view under hyaluronic acid P-WH mixing micropin fluorescence；
Fig. 4 (a) and Fig. 4 (b) is partial enlarged view under hyaluronic acid FITC-P-WH mixing micropin fluorescence.
Specific embodiment
Below in conjunction with attached drawing and technical solution, a specific embodiment of the invention is further illustrated.
A kind of microneedle patch of compensating water and removing wrinkle of the invention is as shown in Figure 1, preparation flow is as shown in Figure 2.
The preparation of 1 epoxy resin microneedles template of embodiment
Highly 500 μm, 500 μm of bottom diameter, 1800 μm of spacing, the microneedles template production of 10 × 14 arrays.Steps are as follows:
1) epoxy resin A glue is mixed with epoxy resin B glue according to mass ratio 3:1 (or volume ratio 2.5:1), stirring to drop
It is fully transparent without wire drawing inside glue, solidify 90min under the conditions of 50 DEG C, make it into maturation stage, obtains semi-solid preparation asphalt mixtures modified by epoxy resin
Adipose membrane.
2) prepared metal micro-needle is placed on semi-solid preparation epoxy resin film, uniformly presses, places 5h under room temperature, obtain
The uniform microneedles template of thickness.
3) measurement gained microneedles template thickness is put in encapsulation at room temperature and is saved, and marks micropin dimensional parameters.
The parameter of microneedles template in the present embodiment be height 500 ± 10 μm, 500 ± 10 μm of bottom diameter, 1800 μm of spacing, battle array
Column micropin number 10 × 14.
The preparation of 2 sample solution of embodiment
The preparation of hyaluronic acid P-WH active peptide mixed liquor, steps are as follows:
1) selecting molecular weight is the hyaluronic acid of 10kDa, 100kDa and 400kDa, is separately added into ultrapure water, is prepared
At 10%, 5%, 2% hyaluronic acid solution, 2h or more is stood after mixing evenly, 4 DEG C of refrigerations is placed it in, stands overnight, make
Its water suction is sufficiently expanded to gel, spare.
2) active peptide P-WH 4mg is weighed, is dissolved in 4ml ultrapure water, is configured to the drug concentration of 1mg/ml, then will
Its concentration for being successively diluted to 800 μ g/ml, 200 μ g/ml, 100 μ g/ml, it is spare.
3) FITC-P-WH (FITC fluorescent tag label active peptide P-WH) 2mg is weighed, the dimethyl sulfoxide of 2ml is dissolved in
In DMSO, it is configured to concentration and is the FITC-P-WH solution of 1mg/ml, then be diluted to 800 μ g/ml, 200 μ g/ml, 100 μ g/
The concentration of ml, it is spare.
4) by the P-WH activity peptide solution of various concentration, FITC-P-WH solution respectively in step 1) various concentration it is saturating
Bright matter acid solution is mixed according to volume ratio 1:4, is stood 2h or more after mixing evenly, is mixed well it, is respectively obtained different dense
The hyaluronic acid P-WH active peptide mixed liquor and hyaluronic acid FITC-P-WH mixed liquor of degree, it is spare.
The soluble empty micropin preparation of 3 hyaluronic acid of embodiment
The preparation of hyaluronic acid solubility micropin, steps are as follows:
1) by the hyaluronic acid solution prepared in embodiment 2 be put under room temperature stand 10min or more it is spare.
2) at room temperature, by molecular weight be 10kDa, 100kDa and 400kDa, concentration be respectively 10%, 5%, 2% it is saturating
1ml is added dropwise respectively and (according to drugloading rate, template area and required microneedle patch thickness, can adjust and solution is added dropwise for bright matter acid solution
Number) on the die cavity of clean epoxy resin microneedles template, guarantee that die cavity is completely covered in hyaluronic acid solution, be vacuum-treated
10min。
3) microneedles template is placed in ventilation natural air drying, demoulds drying, is saved backup for 4 DEG C after sealing.
Embodiment 3 is used to do blank control with embodiment 4.
The preparation of 4 hyaluronic acid P-WH active peptide solubility micropin of embodiment
The preparation of hyaluronic acid P-WH active peptide solubility micropin, steps are as follows:
1) by the hyaluronic acid P-WH active peptide mixed liquor prepared in embodiment 2 be put under room temperature stand 10min with
It is upper spare.
2) 1ml at room temperature, is added dropwise (according to load medicine in the hyaluronic acid P-WH active peptide mixed liquor of various concentration respectively
Amount, template area and required microneedle patch thickness can adjust the number that solution is added dropwise) in clean epoxy resin microneedles template
On die cavity, guarantees that die cavity is completely covered in mixed liquor, be vacuum-treated 20min.
3) microneedles template is placed in ventilation natural air drying, demoulds drying, is saved backup for 4 DEG C after sealing.
Wherein, height 500 μm, 500 μm of bottom diameter, 1800 μm of spacing molecular weight be 10kDa hyaluronic acid P-WH micropin
Under patch fluorescence shown in partial enlargement such as Fig. 3 (a) and Fig. 3 (b).
The preparation of 5 hyaluronic acid FITC-P-WH solubility micropin of embodiment
The preparation of hyaluronic acid FITC-P-WH solubility micropin, steps are as follows:
1) the hyaluronic acid FITC-P-WH mixed liquor prepared in embodiment 2 is put in standing 10min or more under room temperature
It is spare.
2) at room temperature, by the hyaluronic acid FITC-P-WH mixed liquor of various concentration be added dropwise respectively 1ml (according to drugloading rate,
Template area and required microneedle patch thickness can adjust the number that solution is added dropwise) in clean epoxy resin microneedles template mould
On chamber, guarantees that die cavity is completely covered in mixed liquor, be vacuum-treated 30min.
3) microneedles template is placed in ventilation natural air drying, demoulds drying, is saved backup for 4 DEG C after sealing.
Wherein, height 500 μm, 500 μm of bottom diameter, 1800 μm of spacing molecular weight be 10kDa hyaluronic acid FITC-P-WH
Under microneedle patch fluorescence shown in partial enlarged view such as Fig. 4 (a) and Fig. 4 (b).
Embodiment 5 uses hyaluronic acid FITC-P-WH microneedle patch as the compliance test result of embodiment 4, aobvious according to result
Show, after hyaluronic acid is mixed with P-WH active peptide, P-WH can be dispersed in microneedle patch.
Claims (4)
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110025567A (en) * | 2019-05-21 | 2019-07-19 | 南京医科大学康达学院 | A kind of preparation method of autolyzed hyaluronic acid micropin |
| CN111467668A (en) * | 2020-04-17 | 2020-07-31 | 南京鼓楼医院 | Microneedle array with substrate provided with micro sucker structure and preparation method and application thereof |
| CN113813221A (en) * | 2021-10-29 | 2021-12-21 | 湖北省麦诗特生物科技有限公司 | Soluble beauty microneedle patch composition for removing wrinkles and resisting aging of skin and preparation method thereof |
| CN114983857A (en) * | 2022-05-25 | 2022-09-02 | 杭州铂赛生物科技有限公司 | Preparation method and application of pacific codfish skin gelatin peptide microneedle |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005154321A (en) * | 2003-11-25 | 2005-06-16 | Nano Device & System Research Inc | Microneedle for ascorbic acid administration |
| CN108653177A (en) * | 2018-07-20 | 2018-10-16 | 华中科技大学同济医学院附属协和医院 | A kind of microneedle patch and preparation method thereof for whitening spot-removing |
-
2019
- 2019-03-06 CN CN201910166231.2A patent/CN109700691A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005154321A (en) * | 2003-11-25 | 2005-06-16 | Nano Device & System Research Inc | Microneedle for ascorbic acid administration |
| CN108653177A (en) * | 2018-07-20 | 2018-10-16 | 华中科技大学同济医学院附属协和医院 | A kind of microneedle patch and preparation method thereof for whitening spot-removing |
Non-Patent Citations (1)
| Title |
|---|
| 王慧: "类hEGF短肽P-WH在皮肤抗衰老中的功能与机制", 《中国优秀硕士学位论文全文数据库（硕士）基础科学辑》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110025567A (en) * | 2019-05-21 | 2019-07-19 | 南京医科大学康达学院 | A kind of preparation method of autolyzed hyaluronic acid micropin |
| CN111467668A (en) * | 2020-04-17 | 2020-07-31 | 南京鼓楼医院 | Microneedle array with substrate provided with micro sucker structure and preparation method and application thereof |
| CN113813221A (en) * | 2021-10-29 | 2021-12-21 | 湖北省麦诗特生物科技有限公司 | Soluble beauty microneedle patch composition for removing wrinkles and resisting aging of skin and preparation method thereof |
| CN113813221B (en) * | 2021-10-29 | 2023-11-21 | 湖北省麦诗特生物科技有限公司 | Skin wrinkle-removing anti-aging soluble beautifying microneedle patch composition and preparation method thereof |
| CN114983857A (en) * | 2022-05-25 | 2022-09-02 | 杭州铂赛生物科技有限公司 | Preparation method and application of pacific codfish skin gelatin peptide microneedle |
| CN114983857B (en) * | 2022-05-25 | 2023-09-01 | 杭州铂赛生物科技有限公司 | Preparation method and application of Pacific cod skin gelatin peptide microneedle |
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Application publication date: 20190503 |