CN108969880A - A kind of solubility micropin film and preparation method thereof - Google Patents
A kind of solubility micropin film and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
- A61K9/0021—Intradermal administration, e.g. through microneedle arrays, needleless injectors
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
- A61M37/0015—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin by using microneedles
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
- A61M37/0015—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin by using microneedles
- A61M2037/0023—Drug applicators using microneedles
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
- A61M37/0015—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin by using microneedles
- A61M2037/0046—Solid microneedles
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
- A61M37/0015—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin by using microneedles
- A61M2037/0053—Methods for producing microneedles
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Abstract
The invention discloses a kind of soluble micropin films and preparation method thereof, and preparation method includes following operating procedure: A1) preparation of micropin mold；A2) the preparation of premixed solution；A3) the molding of micropin film；A4 it) demoulds, encapsulate, sterilizing.After soluble micropin film is mixed in proportion by Sodium Hyaluronate, water soluble chitosan, other function component, it is prepared according to method provided by the invention.The present invention is produced using the groove structure mold through Laser Micro-Machining, and production process is simple and convenient, suitable for scale production；Solubility micropin film provided by the invention, film-strength are able to satisfy requirement, have moisturizing, promote reparation, antibacterial, whitening and anti-aging function.
Description
Technical field
The present invention relates to beauty and percutaneous drug delivery field, and in particular to a kind of solubility micropin film and preparation method thereof.
Background technique
Micropin technology is widely applied in beauty treatment fields and percutaneous drug delivery field at present.Micropin be it is a kind of by metal,
The materials such as silicon, polymer are made, length is 25~2000 μm, needle point is micron-sized in symmetrical conical or asymmetric scarf
Array structure.Soluble micropin generally by can the high molecular material of biological dissolution or degradation be prepared, be coated on soluble micro-
Bioactive substance in needle is gradually released and is played a role with dissolution of the soluble material in skin.It is soluble micro-
Needle has better biocompatibility and bigger drugloading rate than insolubility micropin, receive more and more attention and grind
Study carefully.Soluble micropin film is widely used on America and Europe, Japan and Korea S and other places, is applied to resisting age of skin, desalination wrinkle, repairs fibre
Significant effect in terms of the beauty such as dimension elasticity, treatment acne, removal dead skin.
Ideal solubility beauty micropin film should have following feature: 1) safety, will not cause to infect；2) it imitates
Fruit property, the effects of anti-aging, whitening can be reached；3) practicability, the strength of materials are met the requirements；4) cost performance, micropin film produced
Journey is simply, conveniently, suitable for scale production.
Currently, the material for being used to prepare soluble micropin film mostlys come from Sodium Hyaluronate, and it is added as needed
Its ingredient is to enhance cosmetic result, such as collagen, polysaccharose substance and glycerol ingredient is added, and can enhance the strong of micropin film
Degree, promotees prosthetic and whitening and other effects at moisture retention.
Sodium Hyaluronate is called sodium hyaluronate, is that one kind is widely present in vertebrate connective tissue, mucous tissue, eyeball
Crystalline lens and certain bacteriums folder film in polysaccharide material, play key player in human skin skin corium, to keep skin
Skin spring function plays an important role.With age, Sodium Hyaluronate loss speed is faster than the speed of growth in skin, leads to flesh
Skin gradually loses gloss elasticity, the aging phenomena such as wrinkle occurs.The Sodium Hyaluronate of studies have shown that exogenous supplement also has machine
The holding skin elasticity function of the original Sodium Hyaluronate of body rises and slows down aging action.It and is a kind of effective by micropin technology
Exogenous supplement Sodium Hyaluronate mode.
It take Sodium Hyaluronate as the micropin of main host material preparation, after it is pierced into human skin, Sodium Hyaluronate will
It dissolves and is stranded in skin under liquid effects, play beautification function.High molecular Sodium Hyaluronate hydrophily is strong, can inhale
The moisture for receiving 1000 times of own vol, can quickly fill and lead up the fine wrinkles of skin, but solution viscosity is with concentration after water swelling
Increase and sharply increase, this is unfavorable for the production of micropin injection molding；Low molecular Sodium Hyaluronate can stimulate in skin in collagen
The generation of albumen increases the elasticity and plumpness of skin, but needle body mechanical strength is not enough to be pierced into skin.It can be seen that only with single point
The Sodium Hyaluronate of son amount cannot meet production and the performance requirement of micropin simultaneously.
The preparation process of soluble micropin mainly by by functional liquid inject micropin mold, drying later, stripping, cutting and
?.Micropin mold is a key factor of micropin production cost, production operation simplicity.Currently, domestic micropin mold is general
After the solid formpiston micropin prepared before this by micro electro mechanical processing or chemical etching, mould is answered by PDMS and obtains former, it will be pre-
Mixed perfusion obtains after dry in former.In this technique, mould is answered by formpiston and is that former process is complicated, prepares micropin to subsequent
Production and processing make troubles, and these preparation methods are complicated, expensive, lead to the increased costs of micropin product, for
The price of consumer is also very high always, and drop is not got off.
In recent years, domestic the preparation of micropin product is had made intensive studies: wherein Publication No. CN103893018A
Sodium Hyaluronate micropin involved in Chinese patent lowers its mechanical performance because joined hyperbranched polysulfones amine or polyester, and
And easy mold release, micropin molding be not poor during the preparation process.The Chinese patent of Publication No. CN102178616B, a kind of sodium hyaluronate are micro-
Needle pad pasting and preparation method thereof, the needle adhesive film are made of sodium hyaluronate, collagen, polyalcohol, efficacy active component etc., but should
Patent is not related to the molecular weight of sodium hyaluronate, causes injection molding in production process difficult.In Chinese patent CN101687090B
Mention hyaluronic acid micropin, but the standby micropin of the patent system is in addition to containing hyaluronic acid, also includes 50%~70% glue
Former albumen.It has been reported that inflammatory reaction, and the patent can be caused after the microneedle patch containing collagen is pierced into skin
Preferably molecular weight is 400,000 or more Sodium Hyaluronate, is also unfavorable for casting film-forming or injects in the micropore of micropin mold.In
It is mentioned in state patent CN104027324 A by chitosan, collagen, the micropin of Sodium Hyaluronate preparation, but the patent provides
Preparation method complex process, be not suitable for industrialization production.Chinese patent CN105078880A and CN201610913515 institute
It joined chitosan and Sodium Hyaluronate in the composition for the micropin film stated, but not clear to the Sodium Hyaluronate of which kind of specification
It limits, it cannot be said that the function of bright hyaluronic acid.It is also mentioned that the hyalomitome of two kinds of molecular weight in Chinese patent CN107184417A
Sour sodium shares, but does not account for antibiotic property, and in addition the patent needs to prepare respectively, pours needle point liquid and substrate liquid, and process is numerous
It is trivial, it is unfavorable for industrialization production.
In conclusion the preparation process of water solubility micropin there has been no a kind of can completely consider safety, practical at present
Property, whitening anti-aging effect and optimize production line technique.
Summary of the invention
The purpose of the present invention is to provide a kind of soluble micropin membrane preparation method, which uses Laser Micro-Machining
Groove structure mold produced, production process is simple to operate, suitable for scale production；Mold energy Reusability, benefit
In reducing cost.Requirement is able to satisfy by the strength of materials of the soluble micropin film of its method preparation.
Present invention simultaneously provides a kind of soluble micropin film, specifically it is to provide and a kind of protects having of generally acknowledging of modern times
Wet, rush repair function Sodium Hyaluronate and the water soluble chitosan composition with moisturizing, rush reparation, antibacterial functions are newly formulated,
And other function ingredient is added, such as glycerol, pentanediol, hexylene glycol, water soluble collagen, epidermal growth factor, vitamin
C, rice bran stoste or arbutin etc. is one or more, and being prepared into has the function of moisturizing, promotees reparation, antibacterial, whitening and anti-aging
Soluble micropin film.
The present invention provides a kind of preparation method of soluble micropin film, including following operating procedure:
A1) the preparation of micropin mold: using laser micro-machining system, and progress laser is micro- on special groove structure mold adds
Work obtains micropin mold, which can Reusability；
A2) the preparation of premixed solution: will be in macromolecule Sodium Hyaluronate, small-molecular-weight Sodium Hyaluronate, water soluble chitosan
It is one or more mixed with purified water, stirring and dissolving；Other function substance is added in the completely backward system of dissolution, as glycerol,
One of pentanediol, hexylene glycol, water soluble collagen, epidermal growth factor, vitamin C, rice bran stoste or arbutin or
It is a variety of, it is uniformly mixed and vacuumizes bubble removing up to premixed solution；
A3) moulding of micropin film, molding: premixed solution is disposably poured into special micropin mold, vacuumizes, dries later
Case is dry；
A4) demould, encapsulate, sterilizing: removing demoulds up to soluble micropin film, cuts, and encapsulation finally carries out irradiation sterilization.
The present invention also advanced optimizes a kind of mold for preparing soluble micropin film: the mold be use by wavelength for
The laser micro-machining system of 355nm purple light carries out Laser Micro-Machining to molding groove structure mold under certain parameter, leads to
Laser power and process velocity are overregulated to adjust the taper and smoothness of micropin length, external morphology, to obtain for making
The mold of standby solubility micropin film, further optimizes soluble micropin film die.
Preferably, the molding groove structure mold includes crescent, rectangle, rectangular, diamond shape, circle or ellipse
Shape, groove height are 5~50mm, 1~100 μm of groove inner surface horizontal departure；
Preferably, the side plate (1) of the micropin mold and microneedle array (2) distance are 5~50mm, and micropin mold height is
10~100mm, micropin die width are 50~1000 mm；
Preferably, the material of the micropin mold includes stainless steel material, polytetrafluoroethylene (PTFE), silica gel, PDMS.
The present invention also provides a kind of soluble micropin films mainly by Sodium Hyaluronate, water soluble chitosan and other function
Group is grouped as；
The Sodium Hyaluronate is 10~100 kDa Sodium Hyaluronate of 500~2000 kDa of macromolecule and small-molecular-weight
One or both of；
The water soluble chitosan is carboxymethyl chitosan, chitosan hydrochloride, chitosan quaternary ammonium salt, chitosan lactate, shell
The one or more of glycan sulfuric ester, chitosan oligosaccharide, hyaluronic acid-like chitosan；
The other function component be glycerol, pentanediol, hexylene glycol, water soluble collagen, epidermal growth factor, vitamin C,
The one or more of rice bran stoste or arbutin.
The macromolecule Sodium Hyaluronate of the solubility micropin film, small-molecular-weight Sodium Hyaluronate, water soluble chitosan,
The weight content of other function component is respectively 0~3%: 0~15%: 0~3%: 0~2%, remaining as purified water
Content.
The micropin component prepares soluble micro- according to a kind of preparation method of soluble micropin film of the application
Needle film, has the feature that
Preferably, the micropin is cone, triangular pyramidal, quadrangle taper, hexagonal pyramidal, crescent；
Preferably, the length of the micropin is 10~3000 μm；
Preferably, the base diameter of the micropin is 50~1000 μm；
Preferably, the spacing of the micropin is 50~3000 μm.
Under the conditions of the specification that the present invention limits, the soluble micropin film for enabling to the present invention to obtain is effective rapidly in time
Functional component is inputted target effect site by ground, is played moisturizing, is promoted reparation, antibacterial, whitening and anti-aging function, while the application
Soluble micropin film have higher-security, will not cause to infect.
In conclusion beneficial effects of the present invention:
1, soluble micropin membrane preparation method provided by the invention, for the mold of structure obtained in Laser Micro-Machining, fluted
On be irrigated the solution of the different component containing beauty functions and prepare micropin film through moulding, molding, demoulding, encapsulation, sterilizing,
This method is simple to operate, suitable for scale production；The mold of structure that Laser Micro-Machining obtains, fluted can make repeatedly
With conducive to production cost is reduced；Requirement is able to satisfy by the strength of materials of the soluble micropin film of its method preparation.
2, the mold that one of present invention prepares soluble micropin film can be prepared by laser micro-machining system,
The taper and smoothness of micropin length, external morphology are adjusted by adjusting laser power and process velocity, so that the present invention can
It to significantly simplify processing step, further reduces the cost, is convenient for industrialization production, there is very strong practicability.
3, the groove height of micropin mold is 5~50mm, can be used for the solution that loading prepares micropin film, is conducive to primary fill
Note；1~100 μm of groove inner surface horizontal departure, conducive to preparation micropin film is smooth, thickness is uniform；The side plate of micropin mold
It (1) is 5~50mm with microneedle array (2) distance, convenient for removing of the micropin film after dry.It is prepared using this micropin mold micro-
Needle film, it is simple to operate, it is suitable for scale production.
4, the present invention passes through the concentration and component for changing micropin premixed solution, and the Sodium Hyaluronate for overcoming small-molecular-weight obtains
The micropin film mechanical strength arrived is inadequate；The Sodium Hyaluronate of macromolecule because viscosity large fluidity is bad, is unable to get knot again
The problem of structure uniform micropin film, the soluble micropin film of intensity and good toughness can be prepared.
5, the small-molecular-weight Sodium Hyaluronate used in the present invention has moisturizing, promotees reparation, anti-inflammatory, bacteriostasis, and water
Also moisturizing, rush reparation, antibacterial action, the two play synergistic effect to soluble chitosan, and cosmetic result is more preferably.
6, the other function component in micropin film of the present invention, glycerol, pentanediol, hexylene glycol play better moisture-keeping function；
Water soluble collagen, epidermal growth factor have good anti-aging effects, while in vitamin C, rice bran stoste, arbutin
Play the role of good whitening.
7, the needle body mechanical strength of micropin film of the present invention is enough, can successfully be pierced into keratoderma, can be effectively by micropin
Film functional component inputs target effect site, works rapidly, can play apparent moisturizing, promote reparation, antibacterial, whitening and anti-ageing
Old effect.
Detailed description of the invention
Fig. 1 micropin die structure dwg.
The micropin membrane sample of Fig. 2 preparation.
Fig. 3 micropin micro-structure diagram.
Specific embodiment
The present invention is further explained combined with specific embodiments below, cited specific embodiment is only several preferred sides
Formula is not construed as limiting the scope of the invention.
Embodiment 1:
The soluble micropin film major function of the present embodiment is moisturizing, promotees reparation and antibacterial, and specific preparation process is as follows:
1, the preparation of micropin mold: using wavelength for the laser micro-machining system of 355nm purple light, contains groove structure special
Stainless steel mould on carry out Laser Micro-Machining, the micropin mold needle point of acquisition is cone shape, and the length of needle point is 100 μ
M, the base diameter of needle point are 600 μm, and the spacing between needle point is 300 μm, the number of per square centimeter on average micropin needle point
About 300；
2, the preparation of premixed solution: by macromolecule Sodium Hyaluronate 1g, small-molecular-weight Sodium Hyaluronate 8g, carboxymethyl chitosan
1g and purified water 89g mixing, stirring and dissolving；Glycerol 1g is added in the completely backward system of dissolution, is uniformly mixed and vacuumizes degasification
It steeps up to premixed solution；
3, the moulding of micropin film, molding: premixed solution is disposably poured into special micropin mold, is vacuumized, drying is dry
It is dry；
4, demould, encapsulate, sterilizing: removing demoulds up to soluble micropin film, cuts, and encapsulation finally carries out irradiation sterilization.
Embodiment 2:
The soluble micropin film major function of the present embodiment is anti-aging, moisturizing, promotees reparation and antibacterial, and specific preparation step is such as
Under:
1, the preparation of micropin mold: using wavelength for the laser micro-machining system of 355nm purple light, contains groove structure special
Teflon mould on carry out Laser Micro-Machining, the micropin mold needle point of acquisition is cone shape, and the length of needle point is
150 μm, the base diameter of needle point is 500 μm, and the spacing between needle point is 600 μm, per square centimeter on average micropin needle point
Number be about 144；
2, the preparation of premixed solution: by macromolecule Sodium Hyaluronate 2g, small-molecular-weight Sodium Hyaluronate 10g, chitosan hydrochloric acid
Salt 1g and purified water 86.4g mixing, stirring and dissolving；Water soluble collagen 0.6g, mixing is added in the completely backward system of dissolution
Uniformly bubble removing is vacuumized up to premixed solution；
3, the moulding of micropin film, molding: premixed solution is disposably poured into special micropin mold, is vacuumized, drying is dry
It is dry；
4, demould, encapsulate, sterilizing: removing demoulds up to soluble micropin film, cuts, and encapsulation finally carries out irradiation sterilization.
Embodiment 3:
The soluble micropin film major function of the present embodiment is whitening, moisturizing, promotees reparation and antibacterial, and specific preparation process is as follows:
1, the preparation of micropin mold: using wavelength for the laser micro-machining system of 355nm purple light, contains groove structure special
PDMS mold on carry out Laser Micro-Machining, the micropin mold needle point of acquisition is cone shape, and the length of needle point is 200 μm,
The base diameter of needle point is 600 μm, and the spacing between needle point is 700 μm, and the number of per square centimeter on average micropin needle point is about
It is 100；
2, the preparation of premixed solution: by macromolecule Sodium Hyaluronate 1g, small-molecular-weight Sodium Hyaluronate 10g, chitosan hydrochloric acid
Salt 3g and purified water 85.0g mixing, stirring and dissolving；Rice bran stoste 0.8g, arbutin 0.2g is added in the completely backward system of dissolution
It is uniformly mixed and vacuumizes bubble removing up to premixed solution；
3, the moulding of micropin film, molding: premixed solution is disposably poured into special micropin mold, is vacuumized, drying is dry
It is dry；
4, demould, encapsulate, sterilizing: removing demoulds up to soluble micropin film, cuts, and encapsulation finally carries out irradiation sterilization.
Embodiment 4:
The morphologic observation of 4.1 films and hardness determination
The micropin film of preparation is placed under 40 times of optical microscopies, micropin form is observed, is shown in Table 1；And use texture
Instrument detects the micropin intensity in different embodiments, the results are shown in Table 1.
Cell toxicity test
Cell toxicity test is carried out according to method as defined in GB/T16886.5~2003 (contact method), is shown in Table 1.
1 Examples 1 to 3 form of table and performance detection
4.3 bacteriostatic test
Hybrid NC machine tool base plate is prepared first, picks them separately a little cultured staphylococcus aureus, green pus with oese
Bacteria suspension is made in sterile saline 5ml in bacillus and Escherichia coli inclined-plane culture substratess.Bacteria suspension 1.0ml is taken to be added to
In the culture dish of sterilizing-drying, addition is cooled to 50 DEG C or so of ordinary nutritional broth agar culture medium, shakes up, to abundant
It is spare after condensation.Then bacteriostatic test is carried out, sample film is cut into the disk of 1.0cm, is affixed on Hybrid NC machine tool base plate,
35~37 DEG C of inversions are cultivated 20~24 hours, are observed bacterial growth situation, are shown in Table 2.
2 Examples 1 to 3 bacteriostatic experiment result of table
4.4 micropin film anti-aging effects
To 10 45-60 years old ages, have obvious crow's feet (wrinkle cannot intersect, and main wrinkle at least 2cm long) from
It is willing to that the side crow's feet position of subject carries out treatment as treatment group using the micropin film of embodiment 2, other side crow's feet is not
Clinical protocol as a control group is treated, half an hour is treated with micropin film sooner or later daily by treatment group, and control group is without controlling
It treats, is measured using the crow's feet after two weeks to treatment group and control group, the crow's feet phase for the treatment of group is found after statistical analysis
Control group is significantly shorter, control group crow's feet average length is 2.6, and control group is not into and the crow's feet for the treatment of group
Crow's feet of the average length in 1.7 treatment groups.Illustrate that the soluble micropin of the invention patent has the anti-wrinkle anti-senescence function of skin.
Micropin film whitening effect
Skin color (skin etc. is measured according to Lab chrominance space as defined in international lighting association to 36 volunteer's skins
Grade classification declaration: it is divided into I, II, III, IV, V totally five ranks from shallow to deep by skin), it, will after pressing skin color classification to subject
Above-mentioned micropin film pressing removes after 4 hours in subject's face, daily using once, observes whitening effect after two weeks: discovery 36
Name subjects skin obtains certain whitening effect, and concrete outcome is shown in Table 3.
3 Examples 1 to 3 whitening effect of table
It should be understood that the foregoing is merely presently preferred embodiments of the present invention, it is not intended to limit the invention, it is all in the present invention
Spirit and principle within, any modification, equivalent replacement, improvement and so on, should be included in protection scope of the present invention it
It is interior.
Claims (8)
1. a kind of preparation method of solubility micropin film, which is characterized in that including following operating procedure:
A1) the preparation of micropin mold；
A2) the preparation of premixed solution；
A3) the molding of micropin film；
A4 it) demoulds, encapsulate, sterilizing.
2. a kind of soluble micropin film as described in claim 1, which is characterized in that
The premixed solution is by one of Sodium Hyaluronate, water soluble chitosan and other function component or a variety of forms；Institute
Micropin mold is stated to be prepared by laser micro-machining system.
3. a kind of soluble micropin film as claimed in claim 2, which is characterized in that
The Sodium Hyaluronate is 10~100 kDa Sodium Hyaluronate of 500~2000 kDa of macromolecule and small-molecular-weight
One or both of；
The water soluble chitosan is carboxymethyl chitosan, chitosan hydrochloride, chitosan quaternary ammonium salt, chitosan lactate, shell
Glycan sulfuric ester, chitosan oligosaccharide, hyaluronic acid-like chitosan it is one or more；
The other function component be glycerol, pentanediol, hexylene glycol, water soluble collagen, epidermal growth factor, vitamin C,
One of rice bran stoste, arbutin are a variety of.
4. a kind of soluble micropin film as claimed in claim 3, which is characterized in that the macromolecule Sodium Hyaluronate, small
Molecular weight hyaluronic acid sodium, water soluble chitosan, other function component weight content be respectively 0~3%: 0~15%
: 0~3%: 0~2%, remaining as the content of purified water.
5. a kind of preparation method of soluble micropin film as described in claim 1, which is characterized in that
The needle point of the micropin film is one of cone, triangular pyramidal, quadrangle taper, crescent；
The length of the micropin needle point is 10~3000 μm；
The base diameter of the micropin needle point is 50~1000 μm；
The spacing of the micropin needle point is 50~3000 μm.
6. a kind of preparation method of soluble micropin film as described in claim 1, which is characterized in that walked including following operation
It is rapid:
A1) the preparation of micropin mold: using wavelength for the laser micro-machining system of 355nm purple light, in special groove structure mould
Laser Micro-Machining is carried out on tool obtains micropin mold；
A2) the preparation of premixed solution: will be in macromolecule Sodium Hyaluronate, small-molecular-weight Sodium Hyaluronate, water soluble chitosan
It is one or more mixed with purified water, stirring and dissolving；Other function component is added in the completely backward system of dissolution, as glycerol,
One of pentanediol, hexylene glycol, water soluble collagen, epidermal growth factor, vitamin C, rice bran stoste or arbutin or
It is a variety of, it is uniformly mixed and vacuumizes bubble removing up to premixed solution；
A3) the molding of micropin film: premixed solution is disposably coated in special micropin mold, vacuumizes by moulding, it is subsequent
It is dry that vacuum environment is held in continuation of insurance；
A4) demould, encapsulate, sterilizing: removing demoulds up to soluble micropin film, cuts, and encapsulation finally carries out irradiation sterilization.
7. a kind of preparation method of soluble micropin film as described in claim 1 or 6, which is characterized in that
The side plate (1) of the micropin mold and microneedle array (2) distance are 5~50mm, micropin mold height is 10~
100mm, micropin die width are 50~1000 mm；
The groove structure of the micropin mold includes crescent, rectangle, rectangular, diamond shape, circle, ellipse, and groove is high
Degree is 5~50mm, 1~100 μm of groove inner surface horizontal departure；
The material of the micropin mold includes stainless steel material, polytetrafluoroethylene (PTFE), silica gel, PDMS.
8. a kind of solubility micropin film, which is characterized in that the soluble micropin film uses claim 1~7 any one
The method is made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810598273.9A CN108969880B (en) | 2018-06-12 | 2018-06-12 | Soluble microneedle film and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810598273.9A CN108969880B (en) | 2018-06-12 | 2018-06-12 | Soluble microneedle film and preparation method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN108969880A true CN108969880A (en) | 2018-12-11 |
| CN108969880B CN108969880B (en) | 2021-03-09 |
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| CN201810598273.9A Active CN108969880B (en) | 2018-06-12 | 2018-06-12 | Soluble microneedle film and preparation method thereof |
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| CN111084929A (en) * | 2019-12-27 | 2020-05-01 | 广东工业大学 | Antibacterial biological membrane composite microneedle and application thereof |
| CN111450405A (en) * | 2020-05-08 | 2020-07-28 | 无锡元旭生物技术有限公司 | Rapid preparation method of soluble microneedle patch stock solution |
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| CN115400341A (en) * | 2022-08-30 | 2022-11-29 | 华中科技大学 | Soluble polymer microneedle and preparation method thereof |
| CN116548205A (en) * | 2023-04-24 | 2023-08-08 | 南京师范大学 | Microneedle preparation, microneedle patch, and preparation methods and applications thereof |
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| CN111084929A (en) * | 2019-12-27 | 2020-05-01 | 广东工业大学 | Antibacterial biological membrane composite microneedle and application thereof |
| CN110917489A (en) * | 2019-12-27 | 2020-03-27 | 广东工业大学 | Antibacterial chitosan microneedle and application thereof |
| CN111450405A (en) * | 2020-05-08 | 2020-07-28 | 无锡元旭生物技术有限公司 | Rapid preparation method of soluble microneedle patch stock solution |
| CN112870089A (en) * | 2021-02-05 | 2021-06-01 | 华东师范大学 | Soluble hyaluronic acid mask with microneedle structure and preparation method thereof |
| CN114228054A (en) * | 2021-12-14 | 2022-03-25 | 优微（珠海）生物科技有限公司 | Microneedle mould and preparation method of microneedle patch |
| CN114228054B (en) * | 2021-12-14 | 2022-08-16 | 优微（珠海）生物科技有限公司 | Microneedle mould and preparation method of microneedle patch |
| CN115400341A (en) * | 2022-08-30 | 2022-11-29 | 华中科技大学 | Soluble polymer microneedle and preparation method thereof |
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| CN116548205A (en) * | 2023-04-24 | 2023-08-08 | 南京师范大学 | Microneedle preparation, microneedle patch, and preparation methods and applications thereof |
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