GB2609691A

GB2609691A - Patterned bacterial cellulose (BC) composite membrane with biological activity, and preparation method and use thereof

Info

Publication number: GB2609691A
Application number: GB2204571.0A
Authority: GB
Inventors: Yang Guang; Oscar Ode Boni Biaou; Li Xiaohong; Lamboni Lallepak
Original assignee: Huazhong University of Science and Technology
Current assignee: Huazhong University of Science and Technology
Priority date: 2021-03-31
Filing date: 2022-03-30
Publication date: 2023-02-15
Also published as: GB202204571D0; CN113069275A

Abstract

The preparation method includes the following steps: inverting a template with a patterned structure into a bacteria culture solution capable of synthesizing bacterial cellulose, and culturing for a period of time to obtain a bacterial cellulose-based membrane with a patterned structure (preferably grooved); and immersing the bacterial cellulose-based membrane into a solution of a natural polymer bioactive material (preferably silk sericin or gelatin) such that the bacterial cellulose-based membrane with a patterned structure and the natural polymer bioactive material are compounded through a hydrogen bond to obtain the patterned bacterial cellulose-based membrane with a biological activity. The composite membrane can be used as a wound dressing to rapidly induce skin tissue regeneration or reduce scarring;

Description

PATTERNED BACTERIAL CELLULOSE-BASED COMPOSITE MEMBRANE WITH

BIOLOGICAL ACTIVITY, AND PREPARATION METHOD AND USE THEREOF

TECHNICAL FIELD

[0001] The present disclosure relates to the technical field of biomedical polymer composites, and particularly, to a patterned bacterial cellulose-based composite membrane with biological activity, as well as the preparation method and its application as a wound dressing.

BACKGROUND ART

[0002] Skin damages can expose the body to various harmful substances and even cause serious blood loss. Therefore, to quickly induce the skin to restore a protective function, researchers have designed various wound dressings to restore a physical barrier function of the skin and promote wound healing by accelerating skin tissue regeneration. However, it is a challenge for the wound dressings to restore the integrity of natural tissue structure of the skin. Some of these dressings lack desirable biological activity and cannot accelerate wound healing, while others can induce the wound healing in a relatively short period of time. This may prevent the damaged skin from restoring to an original normal structure.

[0003] During wound treatment, traditional wound dressings such as gauze are easy to adhere to the wound, and have limited clinical use due to the fact that it is easy to damage the wound surface when exchange the wound dressings. Bacterial cellulose (BC) is a natural hydrogel produced and secreted by microorganisms such as Acelobacter, Rhizobium, and Agrobacterium. Due to the desirable biocompatibility, high water holding capacity, mechanical properties and permeability, and low toxicity, the BC is widely used in artificial skin, wound dressings, cartilage tissue engineering materials, and neurovascular, vascular and dental implant materials. However, due to the lack of biological activity, the efficacy of BC has to be improved in promoting wound healing. Natural polymer bioactive materials such as collagen, gelatin, hyaluronic acid and sericin are rich in sources and have excellent biocompatibility and biological activity. These materials have received more and more attention in the fields of biomedicine and tissue engineering, and have gradually become a research hotspot in the field of natural biomaterials. However, due to the poor mechanical properties when used alone, these materials need to be complexed with other materials to complement their strengths and weaknesses. In addition, remodeling of an extracellular matrix (ECM) plays a crucial role in the repair of skin tissue wounds. The ECM can serve as a dynamic scaffold for protection and promotion of wound repair, and as a signaling molecule for cell growth. Therefore, it is essential for improving the effectiveness and quality of skin repair by precise regulation of biochemical and biomechanical interactions for cell-matrix in space and time. In a previous study, a bacterial cellulose-silk sericin composite (BC-SS) was used as a wound dressing; results of in vitro studies showed that the composite could promote the proliferation of fibroblasts and epithelial cells, thereby promoting wound healing (Lamboni. L, Li.Y, Liu. J and Yang, G. BIOMACROMOT,E,CULES, 2016, 17, 3076-3084.). However, this skin repair dressing may cause uncontrolled proliferation of fibroblasts and lead to the deposition of excessive collagen, resulting in tissue fibrosis and scarring (Keane T. J., Horejs C., Stevens M. M., Adv. Drug Del/v. Rev., 2018, 129: 407-419; Coentro J. Q., Pugliese E., Hanley G., Raghunath M., Zeugolis D. I., Adv. Drug Del/v. Rev., 2019, 146: 37-59.).

SUNIMARY

[0004] The present disclosure solves the problem that the wound repair dressings can lead to uncontrolled proliferation of fibroblasts and lead to the deposition of excessive collagen in the prior art, resulting in tissue fibrosis and scarring. The present disclosure provides a preparation method of a patterned bacterial cellulose-based composite membrane with a biological activity as a wound dressing, where a bacterial cellulose-based membrane with a patterned structure is compounded with a natural polymer bioactive material. A composite membrane prepared by the preparation method has excellent mechanical properties and biological activity, and has a fine groove structure on a surface. The composite membrane can control the orientation arrange and growth morphology of cells, and promote rapid wound healing. The composite membrane can further induce uniform distribution of fibroblasts and deposited collagen, thereby reducing fibrosis and scarring.

[0005] A first aspect of the present disclosure is to provide a preparation method of a patterned bacterial cellulose-based membrane with a biological activity, including the following steps: [0006] (1) inverting a template with a patterned structure into a bacteria culture medium capable of synthesizing bacterial cellulose, and conducting culture to obtain a bacterial cellulose-based membrane with a patterned structure.

100071 (2) immersing the bacterial cellulose-based membrane with a patterned structure obtained in step (1) into a solution of a natural polymer bioactive material, such that the bacterial cellulose-based membrane with a patterned structure and the natural polymer bioactive material are compounded through a hydrogen bond force to obtain the patterned bacterial cellulose-based membrane with a biological activity.

[0008] Preferably, the patterned structure may be a striped groove structure.

[0009] Preferably, the striped groove structure may be formed by grooves of a uniform width, or first grooves and second grooves that have different widths and are alternately arranged; 100101 Preferably, the grooves of a uniform width each may have a width of 5 jun to 20 pm; and the first grooves each may have a width of 5 gm to 15 pm, and the second grooves each may have a width oft pm to 10 pm.

[0011] Preferably, the natural polymer bioactive material may be any one selected from the group consisting of seri ci n, collagen, gelatin, and hyaluroni c acid.

100121 Preferably, the bacteria capable of synthesizing bacterial cellulose may be any one selected from the gyoup consisting of Aceiobacter Mum, Azolobacter, ilgrobacterthm, Rhizobium, Pseudomoncts, and Alcahgenes.

100131 Preferably, in step (1), the culture may be conducted for 5 d to 7 d.

[0014] Preferably, a solute in the solution of the natural polymer bioactive material may have a mass fraction of 1% to 3%; and the immersion may be conducted for 12 h to 48 h. [0015] Preferably, the template with a patterned structure may be a polydimethylsiloxane (PDMS) template with a patterned structure.

[0016] Preferably, a preparation method of the PDMS template with a patterned structure may include the following steps: [0017] Sl: designing a pattern of a lithography mask, and subjecting the designed pattern to lithography on a silicon wafer to obtain a patterned photoresist template; and [0018] S2: fully mixing PDMS and a curing agent, removing air bubbles to obtain a transparent viscous liquid, and pouring the transparent viscous liquid onto the patterned photoresist template obtained in step Si; conducting heat curing, and peeling off the PDMS from the patterned photoresist template, to form the PDMS template with a patterned structure complementary to a structure of the patterned photoresist template.

[0019] A second aspect of the present disclosure is to provide a patterned bacterial cellulose-based membrane with a biological activity prepared by the preparation method.

[0020] A third aspect of the present disclosure is to provide the use of the patterned bacterial cellulose-based membrane with a biological activity in the preparation of a wound dressing; where [0021] preferably, the wound dressing may be used to control the orientation an-ange and growth morphology of cells, and to induce uniform distribution of fibroblasts and deposited collagen, thereby reducing the fibrosis and scarring.

100221 Overall, compared with the prior art, the above technical solutions of the present disclosure mainly have the following technical advantages: [0023] (1) The patterned bacterial cellulose-based membrane has a three-dimensional fiberous network structure, thus providing a spatial structure basis for uniformly compounding the natural polymer bioactive material. The patterned bacterial cellulose-based composite membrane has excellent mechanical properties, and desirable biological activity and biocompatibility.

100241 (2) The patterned bacterial cellulose-based composite membrane has a striped groove structure with an adjustable stripe width, which can guide cells to grow along the fiberous grooves, thereby controlling the orientation arrange and growth morphology of cells.

[0025] (3) The patterned bacterial cellulose-based composite membrane has a fine Groove structure on a surface, which can quickly induce tissue regeneration and promote rapid wound healing, and can induce uniform distribution of fibroblasts and deposited collagen, thereby reducing fibrosis and scarring 100261 (4) Preferably, the striped groove structure is formed by the grooves of a uniform width or alternately arranging the first groove and the second groove with different widths, which helps to adjust the growth and orientation of cells, and to rearrange the microfilaments, microtubules and cytoskeletons. The structure further affects the growth and changes in physiological behavior of cells, which promotes a rapid wound healing, and reduces fibrosis and scarring.

[0027] (5) The preparation method is simple and environmental-friendly, and can be conducted on a large scale.

BRIEF DESCRIPTION OF THE DRAWINGS

[0028] FIG. 1 shows a field emission scanning electron microscope (FESEM) image of an rBC membrane prepared in Comparative Example 1; 100291 FIG. 2 shows an FESEM image of an rBC-SS1 membrane prepared in Comparative Example 2; [0030] FIG. 3 shows an FESEM image of an rBC-SS2 membrane prepared in Comparative Example 2; 100311 FIG. 4 shows an optical microscope image of a PDNIS template with uniform groove width (10 um) prepared in Example 1; [0032] FIG. 5 shows an FESEM image of an mBC membrane prepared in Example 2; [0033] FIG. 6 shows an FESEM image of an mBC-SS1 membrane prepared in Example 4; 100341 FIG. 7 shows an FESEM image of an mBC-SS2 membrane prepared in Example 5; [0035] FIG. 8 shows test results of a relative cell proliferation rate of samples prepared in Comparative Examples 1-3 and Examples 5-7; 100361 FIG. 9 shows a laser confoca1 microscope image of NIFI-3T3 cells in the samples prepared in Comparative Examples 1-3 and Examples 5-7; [0037] FIG. 10 shows a digital photo of the samples prepared in Comparative Examples 1-3 and Example 5-7 in repairing rat skin damages; 100381 FIG. 11 shows quantitative test results of a wound healing rate of the samples prepared in Comparative Examples 1-3 and Example 5-7 in repairing the rat skin damages 100391 FIG. 12 shows H&E staining results of the samples prepared in Comparative Examples 1-3 and Example 5-7 in repairing the rat skin damages for 3 d; where a refers to a blank control, b refers to the rBC, c refers to the mBC, d refers to the rBC-SS2, e refers to the mBC-SS2, and f refers to the mBC-SS; 100401 FIG. 13 shows test results of a thickness of granulation tissues formed after the samples prepared in Comparative Examples 1-3 and Example 5-7 in repairing the rat skin damages for different days; 100411 FIG. 14 shows test results of a thickness of epidermis formed after the samples prepared in Comparative Examples 1-3 and Example 5-7 in repairing the rat skin damages for different days; [0042] FIG. 15 shows collagen-III immunohistochemical fluorescence staining images of the samples prepared in Comparative Examples 1-3 and Example 5-7 in repairing the rat skin damages for 7 d and 14 d; and [0043] FIG. 16 shows quantitative determination results of collagen-III synthesis of the samples prepared in Comparative Examples 1-3 and Example 5-7 in repairing the rat skin damages for 7 d and 14 d.

DETAILED DESCRIPTION OF THE EMBODIMENTS

100441 In order to make the aim, technical solutions, and advantages of the present disclosure more clear, the present disclosure is further described in detail below with reference to the figures and examples. It should be appreciated that the specific example described herein is only intended to explain the present disclosure and is not intended to limit the present disclosure. Further, the technical features involved in the various embodiments of the present disclosure described below may be combined with each other as long as they do not constitute a conflict with each other.

[0045] Comparative Example 1 100461 A preparation method of a BC membrane included: a Hestrin & Schramm medium was prepared, where each component and proportion were: citric acid (0.15%, w/v), disodium phosphate (0.27%, w/v), a yeast extract (0.5%, w/v), peptone (0.5%, w/v), and glucose (2%, w/v). Each of the component was dissolved in deionized water, sterilized at high temperature, and AceThhacter xylinum was inoculated in a sterilized medium, followed by conducting incubation in an incubator at 30 °C for 7 d. The boiling was conducted with a 1N4 NaOH solution to remove bacteria remained in the BC membrane, and the BC membrane was washed with the deionized water several times until a pH value was 7. FIG. 1 shows an FESEM image of a rBC (random ) BC) membrane. As can be seen from the figure, the nanoflbers are randomly arranged to form a network structure that communicates with each other.

[0047] Comparative Example 2 [0048] A preparation method of a BC/SS (silk sericin) composite membrane included: silkworm cocoons were cut into pieces, boiled at 120°C. for 1 h, and a solution was concentrated by dialysis, and freeze-dried to obtain an SS powder. SS solutions with mass fractions of 1%, 2% and 3% were prepared with deionized water, added to a BC membrane, immersed at room temperature for 24 h to obtain a BC/SS composite membrane, followed by washing with PBS to remove excessive SS. FIG. 2 and FIG. 3 are FESEM images of rBC-SS1 and rBC-552 membranes, respectively. It can be seen that the SS is uniformly distributed on a surface of the rBC membrane.

[0049] Example 1

[0050] Patterned design and a preparation method of a PDMS template included the following steps: [0051] (1) Uniform striped groove patterns with a width of 10 pm, or alternately-arranged striped groove patterns with a width of 10 m and 5 pm were designed using AutoCAD drawing software. The designed patterns were subjected to lithography on a silicon wafer, and a patterned surface was silanized by perfluorosilane to enhance the inertness of the patterned template surface to prolong a service life.

[0052] (2) two liquids, PDMS and a curing agent, were fully mixed according to a mass ratio of 10:1, and air bubbles were removed to obtain a transparent viscous liquid; bubble-removed PDMS was poured onto a patterned photoresist template with a thickness of 3 mm, and the air bubbles were further removed by vacuum.

[0053] (3) The template was placed in an oven to fully cure the PDMS, and the PDMS was carefully peeled off from the patterned photoresist template; the PDMS formed a pattern complementary to the template in concavity and convexity, and the pattern was cut into a required size for further use.

[0054] FIG. 4 shows an optical microscope image of the PDMS template grooves with a uniform width (10 pm). It can be seen from the figure that, the stripe structure of the groove is arranged in parallel, with a same width of each stripe.

[0055] Example 2

[0056] A preparation method of a patterned BC membrane included: a Hestrin & Schramm medium was prepared, where each component and proportion were: citric acid (0.15s4, w/v), disodium phosphate (0.27%, w/v), a yeast extract (0.5%, w/v), peptone (0.5%, w/v), and glucose (2%, w/v). Each of the component was dissolved in deionized water, sterilized at high temperature, and Acetobacter xylitutm was inoculated in a sterilized medium, a PDMS template with a stripe width of 10 um was inverted in the medium, followed by conducting incubation in a 30°C incubator for 7 d. Boiling was conducted with a 1M NaOH solution to remove bacteria remaining in the BC membrane, and the BC membrane was washed with the deionized water several times until a pH value was 7. FIG. 5 shows a FESEM image of the mBC membrane. It can be seen from the figure that the mBC is a neatly-arranged fiberous groove stnicture with a relatively dense directional arrangement.

100571 Example 3

[0058] A Hestrin & Schramm medium was prepared, where each component and proportion were: citric acid (0.15%, w/v), disodium phosphate (0.27%, w/v), a yeast extract (0.5%, w/v), peptone (0.5%, w/v), and glucose (2%, w/v). Each of the component was dissolved in deionized water, sterilized at high temperature, and Acetobacter xylinum was inoculated in a sterilized medium, alternately-arranged striped groove PDMS templates with a width of 10 um and 5 [trn were inverted in the medium, followed by conducting incubation in a 30°C incubator for 7 d. Boiling was conducted with a 1M NaOH solution to remove bacteria remaining in the BC membrane, and the BC membrane was washed with the deionized water several times until a pt-I value was 7, to obtain alternately-arranged fiberous groove structure membranes with different widths.

[0059] Example 4

100601 A preparation method of a patterned BC/SS composite membrane included the following steps: [0061] Silkworm cocoons were cut into pieces, boiled at 120°C for 1 h, and a solution was concentrated by dialysis, and freeze-dried to obtain an SS powder. An SS solution with a mass fraction of 1% was prepared with deionized water, added to a patterned BC membrane with a stripe width of 10 tun, immersed at room temperature for 24 h to obtain a patterned BC/SS composite membrane, followed by washing with PBS to remove excessive SS. FIG. 6 shows a FESEM image of an mBC-SS1 membrane It can be seen from the figure that the SS is uniformly compounded in the BC membrane.

[0062] Example 5

[0063] A preparation method of a patterned BC/SS composite membrane included the following steps: [0064] Silkworm cocoons were cut into pieces, boiled at 120°C for 1 h, and a solution was concentrated by dialysis, and freeze-dried to obtain an SS powder. An SS solution with a mass fraction of 2% was prepared with deionized water, added to a patterned BC membrane with a stripe width of 10 i.tm, immersed at room temperature for 24 h to obtain a patterned BC/SS composite membrane, followed by washing with PBS to remove excessive SS. FIG. 7 shows an FESENI image of an mBC-SS2 membrane.

[0065] Example 6

[0066] A preparation method of a patterned BC/SS composite membrane included: silkworm cocoons were cut into pieces, boiled at 120°C for 1 h, and a solution was concentrated by dialysis, and freeze-dried to obtain an SS powder. An SS solution with a mass fraction of 3% was prepared with deionized water, added to a patterned BC membrane with a stripe width of 10 pm, immersed at room temperature for 24 h to obtain an mBC-SS composite membrane, followed by washing with PBS to remove excessive SS.

[0067] Example 7

[0068] 0.2 g of collagen was dissolved in 10 mL of deionized water by stirring. After being completely dissolved, a collagen solution was added to an mBC membrane with a groove width of 10)tm, immersed at room temperature for 24 h to obtain a patterned BC-collagen composite membrane, followed by washing with PBS to remove excessive collagen to obtain a collagen-modified mBC functional membrane.

[0069] Example 8

[0070] 0.2 g of gelatin was dissolved in 10 mL of deionized water by stirring. After being completely dissolved, a gelatin solution was added to an mBC membrane with a groove width of 10 gm, immersed at room temperature for 24 h to obtain a patterned BC-gelatin composite membrane, followed by washing with PBS to remove excessive gelatin to obtain a gelatin-modified mBC functional membrane.

[0071] Example 9

[0072] 0.2 g of hyaluronic acid was dissolved in 10 mL of deionized water by stirring. After being completely dissolved, a hyaluronic acid solution was added to a patterned BC membrane with a first groove in 5 pm width and a second groove in 10 pm width that were alternately-arranged, immersed at room temperature for 24 h to obtain an mBC-hyaluronic acid composite membrane, followed by washing with PBS to remove excessive hyaluronic acid to obtain a hyaluronic acid-modified mBC functional membrane.

[0073] Example 10

[0074] The cytocompatibility of composite membranes mBC, mBC-SS1, mBC-SS2, and mBCSS3 prepared in Examples 1, 2, 3, and 4, as well as composite membranes rBC, rBC-SS1, rBCSS2, and rBC-SS3 prepared in Comparative Examples 1 and 2 were evaluated using NIH-3T3 (mouse embryonic fibroblast cell line) as a model cell. The two types of cells were cultured in a CO, incubator at 37°C and 5% CO2; when the cell fusion reached to 80% to 90%, the cells were seeded on each group of the materials, with an amount of the cells (6x 1040 seeded on each material, after 1 d, 3 d, and 5 d of culture, a cell viability assay was conducted with CCK-8, where each sample was repeated three times; and a relative cell proliferation rate was calculated. FIG. 8 and FIG. 9 show test results of a relative cell proliferation rate and a laser confocal microscope image of NIH-3T3 cells grown on the materials in the samples prepared in Comparative Examples 1-3 and Examples 5-7. It can be seen from the figures that the mBC composite membrane can promote cell proliferation more than the rBC composite membrane, and can guide cells to grow along the fiberous grooves, indicating that the mBC composite membrane has a desirable potential for use in inducing tissue oriented growth.

100751 Example 11

[0076] Approved by the Laboratory Animal Experiment Ethics Committee of Wuhan Servicebio Technology Co., Ltd., adult male Wistar rats were used for in vivo experiments. 16 adult male Wistar rats weighing 300 g to 500 g were randomly divided into four groups: 3 d, 7 d, 14 d, and 21 d according to an experimental time point. The rats were anesthetized, and traumatic surgery was conducted following standard aseptic procedures. The back of rat was shaved to create 6 circular wounds of 9 mm in diameter. All 6 wounds were treated with different dressings including mBC, mBC-SS1, mBC-SS2, rBC, rBC-SS2 and gauze (as a control). A microstructured surface of the mBC and its composite were in contact with the wound, while a smooth surface of the rBC and its composite were in contact with the wound. According to preliminary test results of 7 d, the dressing was changed every 48 h for the first 7 d, and every 24 h for the rest of the time. According to the surface structure of the BC and its composites, the dressing was kept in the wound for 48 h to induce the distribution of cells, especially fibroblasts. FIG. 10 shows a digital photo of the samples of each group in repairing rat skin damages; FIG. 11 shows corresponding quantitative test results of a wound healing rate. It can be seen from the figures that the mBC and rBC composite membrane groups have higher wound healing speed and wound healing rate than that of the control group, showing great advantages in skin damage repair. FIG. 12 shows H&E staining results of the samples of each group in repairing the rat skin damages for 3 d; where a refers to a blank control, b refers to the rBC, c refers to the mBC, d refers to the rBC-SS2, e refers to the mBC-SS2, and f refers to the mBC-SS1. It can be seen that obvious granulation tissues were seen in the rBC and mBC groups, while no granulation tissue was formed in the blank control group. Furthermore, the rBC-SS2, mBC-SS1 and mBC-SS2 composite groups present more significant granulation tissue formation relative to the rBC group. From the results of quantitative analysis on granulation tissue formation (FIG. 13), it is found that the thickness of granulation tissue formation increases in different groups on 7th day; on 14th and 21th days, the thickness of granulation tissue formation decreases. The reason is that in each group, the transition of skin tissue from hyperplasia to remodeling is more obvious in mBC and mBC-SS2 groups than that in rBC and rBC-SS2 groups. This indicates that the mBC-SS2 composite can effectively inhibit the overgrowth of new tissues. FIG. 14 shows results of a quantitative test of epidermal layer thickness in skin repair. On the 7th day, except for the total lesion skin group (control) without epithelization, the rest groups have obvious epithelization. On days 14 and 21, the thickness of nascent epidermis on the different BC groups is reduced relative to that on day 7, possibly due to the transformation of proliferated keratinocytes into keratinized cells during the epidermal remodeling. In addition, FIG. 15 and FIG. 16 show the immunohistochemical fluorescence staining and quantitative test results of collagen-III production in different groups, respectively. From the experimental results, it is found that on day 7, except for the blank control group and the single BC group, the other groups significantly promote the secretion of collagen-III, indicating that the compounding of sericin improves the synthesis of collagen. On the 14th day, the secretion of collagen-III in the mBC and mBC-SS2 groups is lower than that in the other groups, and the synthesis amount of collagen-III in the mBC-SS group is significantly lower than that in the other groups. This indicates that there is no excessive wound contraction in the mBC-SS group relative to the other groups, indirectly reflecting the prevention of fibrosis and scarring in this group.

100771 It is easy for those skilled in the art to understand that the above-mentioned contents are merely the preferred examples of the present disclosure, and are not intended to limit the present disclosure. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present disclosure should fall within the protection scope of the present disclosure.

Claims

WHAT IS CLAIMED IS: 1. A preparation method of a patterned bacterial cellulose-based membrane with a biological activity, comprising the following steps: (1) inverting a template with a patterned structure into a bacteria culture medium capable of synthesizing bacterial cellulose, and conducting culture to obtain a bacterial cellulose-based membrane with a patterned structure; and (2) immersing the bacterial cellulose-based membrane with a patterned structure obtained in step (1) into a solution of a natural polymer bioactive material, such that the bacterial cellulose-based membrane with a patterned structure and the natural polymer bioactive material are compounded through a hydrogen bond force to obtain the patterned bacterial cellulose-based membrane with a biological activity.
2. The preparation method of a patterned bacterial cellulose-based membrane with a biological activity according to claim 1, wherein the patterned structure is a striped groove structure.
3. The preparation method of a patterned bacterial cellulose-based membrane with a biological activity according to claim 2, wherein the striped groove structure is formed by grooves of a uniform width, or first grooves and second grooves that have different widths and are alternately arranged; preferably, the grooves of a uniform width each have a width of 5 um to 20 gm; and the first grooves each have a width of 5 pm to 15 gm, and the second groove each have a width of 1 um to 10 um
4. The preparation method of a patterned bacterial cellulose-based membrane with a biological activity according to claim 1, wherein the natural polymer bioactive material is any one selected from the group consisting of sericin, collagen, gelatin, and hyaluronic acid; and preferably, the bacteria capable of synthesizing bacterial cellulose are any one selected from the group consisting of Acetohacter xylinum, Azotohacter, Agrohacterium, Rhizohium, I' sendomonas, and Alcahgenes.
5. The preparation method of a patterned bacterial cellulose-based membrane with a biological activity according to claim 1, wherein in step (1), the culture is conducted for 5 d to 7 d.
6. The preparation method of a patterned bacterial cellulose-based membrane with a biological activity according to claim I, wherein a solute in the solution of the natural polymer bioactive material has a mass fraction of 1% to 3%, and the immersion is conducted for 12 h to 48 h.
7. The preparation method of a patterned bacterial cellulose-based membrane with a biological activity according to claim 1, wherein the template with a patterned structure is a polydimethylsiloxane (PDMS) template with a patterned structure
8. The preparation method of a patterned bacterial cellulose-based membrane with a biological activity according to claim 7, wherein a preparation method of the PDMS template with a patterned structure comprises the following steps: Si: designing a pattern of a lithography mask, and subjecting the designed pattern to lithography on a silicon wafer to obtain a patterned photoresist template; and S2: fully mixing PDMS and a curing agent, removing air bubbles to obtain a transparent viscous liquid, and pouring the transparent viscous liquid onto the patterned photoresist template obtained in step Si; conducting heat curing, and peeling off the PDMS from the patterned photoresist template, to form the PDMS template with a patterned structure complementary to a structure of the patterned photoresist template.
9. A patterned bacterial cellulose-based membrane with a biological activity prepared by the preparation method according to any one of claims 1 to 8.
10. Use of the patterned bacterial cellulose-based membrane with a biological activity according to claim 9 in the preparation of a wound dressing; wherein preferably, the wound dressing is used to control the orientation arrange and growth morphology of cells, and to induce uniform distribution of fibroblasts and deposited collagen, thereby reducing fibrosis and scarring.