CN109679258A - Blood vessel casting agent, toughness blood vessel casting sample and preparation method thereof - Google Patents
Blood vessel casting agent, toughness blood vessel casting sample and preparation method thereof Download PDFInfo
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- CN109679258A CN109679258A CN201811642232.1A CN201811642232A CN109679258A CN 109679258 A CN109679258 A CN 109679258A CN 201811642232 A CN201811642232 A CN 201811642232A CN 109679258 A CN109679258 A CN 109679258A
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- C—CHEMISTRY; METALLURGY
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- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L27/00—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a halogen; Compositions of derivatives of such polymers
- C08L27/22—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a halogen; Compositions of derivatives of such polymers modified by chemical after-treatment
- C08L27/24—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a halogen; Compositions of derivatives of such polymers modified by chemical after-treatment halogenated
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- G—PHYSICS
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- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/28—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for medicine
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Abstract
The present invention relates to a kind of blood vessel casting agents, toughness blood vessel casting sample and preparation method thereof, belong to bioencapsulation technical field.The blood vessel casting agent, the component including following mass fraction: 100 parts of mold material, 15~30 parts of solvent, 0.3~0.8 part of plasticizer, 3~7 parts of softening agent；Mold material is one or more of chliorinated polyvinyl chloride, Vinylidene Chloride or polyvinyl chloride；Softening agent is one or more of liquid nitrile rubber, liquid polysulphide rubber.The toughness blood vessel casting sample made using the blood vessel casting agent, toughness is preferable, and long-term preservation is unlikely to deform.
Description
Technical field
The invention belongs to bioencapsulation technical fields, and in particular to a kind of blood vessel casting agent, toughness blood vessel casting sample and
Preparation method.
Background technique
It imparts knowledge to students in school at present, the common blood vessel casting sample of study has: dipping specimen and archetype.Archetype structure
Simply, have certain deviation between teaching and true sample, can not achieve sample by shallow-layer to deep structure title display.Leaching
The bottled preparation of specimen's process of stain is simple, needs to save in a liquid, cannot be exposed in air for a long time, be not easy observational learning,
And toxic, irritant smell, and blood vessel easily snaps off in use, influences sample beauty.
Cast moulds technical application has nearly 20 years history to biological dissec field, is produced using cast technique
Come blood vessel casting sample have it is nontoxic, it is tasteless, can save for a long time, it is easy to use, can long-term preservation in the medium advantage of air.
Cast agent injection is intravascular, with solution corrosion tissue appropriate and removes after cast agent solidification, is only stayed
Under filler cast moulds.The cast agent used in blood vessel casting technology usually has tooth shedding, ABS engineering plastics, first
Base methyl acrylate, polyvinyl chloride and Vinylidene Chloride etc., while assisting making cast agent curing molding with plasticiser, solvent etc..
After existing cast agent injects blood vessel and forms, obtained blood vessel casting sample is generally brittle, and toughness is poor,
It is easy to appear cracking when saving for a long time, is not easy to save for a long time.
Summary of the invention
The object of the present invention is to provide a kind of blood vessel casting agents, and the blood vessel casting agent toughness is preferable, the blood vessel casting of preparation
Sample has certain toughness, and long-term preservation is indeformable.
A second object of the present invention is to provide a kind of preparation methods of toughness blood vessel casting sample.
Third object of the present invention is to provide a kind of toughness blood vessel casting samples.
In order to achieve the goal above, the technical scheme adopted by the invention is as follows: blood vessel casting agent, including following mass fraction
Component:
100 parts of mold material, 15~30 parts of solvent, 0.3~0.8 part of plasticizer, 3~7 parts of softening agent；
Mold material is one or more of chliorinated polyvinyl chloride, Vinylidene Chloride or polyvinyl chloride；
Softening agent is one or more of liquid nitrile rubber, liquid polysulphide rubber.
Further, the solvent is ethyl acetate or acetone.
Further, the plasticizer is dihexyl adipate or dibutyl phthalate.
Further, the softening agent is the mixture of liquid nitrile rubber and liquid polysulphide rubber, liquid nitrile rubber
Mass ratio with liquid polysulphide rubber is 3:1~5:1.
Further, mold material is chliorinated polyvinyl chloride.
Further, the blood vessel casting agent further includes the fat-soluble pigment of 0.8~1.2 mass fraction, fat-soluble pigment
For acrylic paints.
Using the method for above-mentioned blood vessel casting agent preparation toughness blood vessel casting sample, comprising the following steps:
1) prepare blood vessel casting agent, take mold material, solvent, plasticizer, the softening agent of formula ratio, be uniformly mixed to get
Blood vessel casting agent；
2) Perfused vessel cast agent marks the biology that blood vessel casting agent prepared in step 1) is once perfused to after being intubated
In this, secondary pouring is carried out after being spaced 1~3h, the blood vessel casting sample after must being perfused；
3) corrode, by blood vessel casting sample 12~14h of standing after perfusion in step 2) so that blood vessel casting agent solidifies, it
It is placed on 5~15d of corrosion, the blood vessel casting sample after must corroding in hydrochloric acid；
4) bleach, the blood vessel casting sample after the corrosion in step 3) rinsed well, be placed in hydrogen peroxide bleaching 2~
3d, up to toughness blood vessel casting sample after finishing.
Further, secondary pouring described in step 2) is supplement perfusion, and the number for supplementing perfusion is 2~3 times.
Further, the percent concentration of hydrochloric acid described in step 3) is 15~25%, hydrogen peroxide described in step 4)
Mass concentration is 8~12%.
The toughness blood vessel casting sample prepared using above-mentioned blood vessel casting agent.
Each substance source is as follows in above-mentioned technical proposal:
Liquid nitrile rubber is purchased from Shanghai Ju Dao Chemical Co., Ltd..
Liquid polysulphide rubber is purchased from Jinxi Research Institute of Chemical Industry Co., Ltd..
Vinylidene Chloride is purchased from Jinan Jun Teng Chemical Co., Ltd..
Chliorinated polyvinyl chloride believes chemical limited liability company purchased from Shandong height.
Polyvinyl chloride is purchased from ten thousand occasion Chemical Co., Ltd. of Guangdong.
Beneficial effects of the present invention:
Blood vessel casting agent of the invention, the chliorinated polyvinyl chloride contained are to be made by polyvinyl chloride by chlorination modified.Chlorine
Change it is modified, material molecule key polarity increase, intermolecular force enhancing, chemical stability increase, make material mechanical property,
Resistance to chemical corrosion and flame retardant property are greatly improved.
Blood can be improved such as liquid nitrile rubber, liquid polysulphide rubber in blood vessel casting agent of the invention, the softening agent contained
The toughness of pipe cast agent.Rubber can be precipitated in liquid nitrile rubber or liquid polysulphide rubber during chliorinated polyvinyl chloride is cured
Particle forms dispersed phase with chliorinated polyvinyl chloride, and rubber grain and chliorinated polyvinyl chloride practise physiognomy and have chemical key connection, and formation is conducive to
Enhancing, toughening and finely dispersed " island structure ".The sample made using blood vessel casting agent of the invention is by certain external force
When deforming, it can restore rapidly.
Blood vessel casting agent of the invention, the plasticizer contained can be with such as dihexyl adipate or dibutyl phthalate
Softening agent, chliorinated polyvinyl chloride cooperate, and improve the toughness of blood vessel casting agent.Dibutyl phthalate is chlorinated polyvinyl chloride second
The common plasticizer of alkene may make chliorinated polyvinyl chloride solidification finished product to have good flexibility.
Toughness blood vessel casting sample of the invention is made of true biology, and the general shape of sample is without change, really
Sense is strong, can clearly show that the neighbouring relationship between position, form and the big and small vessel of blood vessel, facilitate look at, facilitate teacher
Teaching and student's study, provide reference for clinical operation.Toughness blood vessel casting sample of the invention, it is non-toxic and tasteless, it can be for a long time
Be placed in air, be convenient for long-term preservation, be used multiple times, teacher, student can it is hand held at any time in watch and learn, be not required to
It is encapsulated in glass case and impregnates.Toughness blood vessel casting sample of the invention, flexibility is preferable, is easy to use and protects for a long time
It deposits.Specifically in use, being easy to use related equipment to carry out operational demonstration to toughness blood vessel casting sample, such as in teaching process
Pricked using needle etc..
Detailed description of the invention
Fig. 1 is upper limb toughness blood vessel casting sample figure in embodiment 1；
Fig. 2 is upper limb toughness blood vessel casting sample figure in embodiment 1 (drawing to another state).
Specific embodiment
Below in conjunction with embodiment and attached drawing, the invention will be further described.
Embodiment 1
The blood vessel casting agent of the present embodiment, including following component:
Chliorinated polyvinyl chloride 100kg, solvent 50kg, plasticizer 0.5kg, softening agent 5kg, fat-soluble pigment 1kg.
Solvent is ethyl acetate.
Plasticizer is dibutyl phthalate.
Softening agent is liquid nitrile rubber.
Fat-soluble pigment is acrylic paints, and fat-soluble pigment includes red pigment and blue pigment.
The preparation method of the toughness blood vessel casting sample of the present embodiment, comprising the following steps:
1) selection, select skin it is complete, without wound, without fracture, without lesion, the upper limb specimens without corruption.
2) prepare blood vessel casting agent, take mold material, solvent, plasticizer, the softening agent of formula ratio, be uniformly mixed to get
Blood vessel casting agent.When preparing blood vessel casting agent, the blood vessel casting agent of two kinds of colors is prepared, including use made of red pigment and be in
The blood vessel casting agent of existing red blood vessel casting agent and the presentation blue prepared using blue pigment.
3) it is intubated, main artery, big vein is found in upper limb specimens, finds subclavian artery at upper limb specimens armpit,
It is inserted into blood vessel with bore 0.3cm plastic cannula, is tightened with surgical thread, finds ulnar artery, radial artery, clavicle in upper limb specimens forearm
Lower vein, hand back vein etc. tighten, the upper limb specimens after must being intubated with bore 0.1cm plastic cannula insertion blood vessel with surgical thread.
4) Perfused vessel cast agent is drained the residual liquid in the blood vessel in the upper limb specimens after intubation with syringe
Only.Later, blood vessel casting agent prepared in step 2) is once perfused in the upper limb specimens to after being intubated using syringe, root
Groundwater increment is determined according to the volume size of upper limb specimens, age size.Primary perfusion 200mL blood vessel casting agent.It is carried out after the 1h of interval
Secondary pouring, secondary pouring are supplement perfusion, and the number for supplementing perfusion is 2 times, and the amount of supplement perfusion is 200mL every time, altogether
600mL, the upper limb blood vessel casting sample after must being perfused is perfused.In filling process, the pressure of strict control blood vessel, when perfusion
Speed is slow, as long as there is pressure that will stop being perfused, to prevent the excessive blood vessel explosion of pressure.Red blood is presented in arteries perfusion
The blood vessel casting agent of blue is presented in pipe cast agent, vein blood vessel perfusion.
5) corrode, the blood vessel casting sample in step 4) after perfusion stood into 12h so that blood vessel casting agent solidifies, postposition
Corrode 5~7d in the hydrochloric acid that percent concentration is 20%, control corrosion rate temperature is 20~35 DEG C, until the upper limb after perfusion
The institutional framework corrosion of blood vessel casting sample is complete, the upper limb blood vessel casting sample after must corroding.
6) it rinses, with tap water gently the residual miscellaneous tissue wash on the upper limb blood vessel casting sample after corrosion is clean,
The upper limb blood vessel casting sample that must be rinsed well.
7) it bleaches, the upper limb blood vessel casting sample rinsed well is placed in the hydrogen peroxide solution that mass fraction is 10% and is floated
White 2 days, the upper limb blood vessel casting sample after must bleaching.
8) it modifies, the upper limb blood vessel casting sample after bleaching is rinsed well and dried, later removes vascular clot, dredge
It is close to beat branch, heat orthopedic, fixed morphologic localization, disconnected to draw glass glue and glue, it is good that finishing will reach permeability, big blood vessel, small
Vascular distribution is uniform, the vivid effect of color.
The toughness blood vessel casting sample of the present embodiment is prepared by the preparation method of the toughness blood vessel casting of the present embodiment.
Embodiment 2
The blood vessel casting agent of the present embodiment, including following component:
Polyvinyl chloride 100kg, solvent 40kg, plasticizer 0.7kg, softening agent 4kg, fat-soluble pigment 0.8kg.
Solvent is ethyl acetate.
Plasticizer is dihexyl adipate.
Softening agent is the mixture of liquid nitrile rubber and liquid polysulphide rubber, liquid nitrile rubber and liquid polysulphide rubber
Mass ratio be 3:1.
Fat-soluble pigment is acrylic paints, and fat-soluble pigment includes red pigment and blue pigment.
The preparation method of the toughness blood vessel casting sample of the present embodiment, comprising the following steps:
1) selection is selected without lesion, the liver specimens without corruption.
2) prepare blood vessel casting agent, take mold material, solvent, plasticizer, the softening agent of formula ratio, be uniformly mixed to get
Blood vessel casting agent.When preparing blood vessel casting agent, the blood vessel casting agent of two kinds of colors is prepared, including use made of red pigment and be in
The blood vessel casting agent of existing red blood vessel casting agent and the presentation blue prepared using blue pigment.
3) it is intubated, arteria hepatica is found in liver specimens, vena portae hepatica is inserted into blood vessel with plastic cannula, is pricked with surgical thread
Tightly, the liver specimens after must being intubated.Red blood vessel casting agent is presented in arteries perfusion, and blue is presented in vein blood vessel perfusion
Blood vessel casting agent.
4) Perfused vessel cast agent is drained the residual liquid in the blood vessel in the liver specimens after intubation with syringe
Only.Later, blood vessel casting agent prepared in step 2) is once perfused in the liver specimens to after being intubated using syringe, root
Groundwater increment is determined according to the volume size of liver specimens, age size.Primary perfusion 200mL blood vessel casting agent.It is carried out after the 1h of interval
Secondary pouring, secondary pouring are supplement perfusion, and the number for supplementing perfusion is 3 times, and the amount of supplement perfusion is 200mL every time, altogether
800mL, the liver vessel cast moulds after must being perfused are perfused.In filling process, the pressure of strict control blood vessel, when perfusion
Speed is slow, as long as there is pressure that will stop being perfused, to prevent the excessive blood vessel explosion of pressure.
5) corrode, by the liver vessel cast moulds standing 12h after perfusion in step 4) so that blood vessel casting agent solidifies, it
It is placed in the hydrochloric acid that percent concentration is 15% and corrodes 10~15d, control corrosion rate temperature is 5~12 DEG C, until after perfusion
Liver vessel cast moulds institutional framework corrosion completely, the upper limb blood vessel casting sample after must corroding.
6) it rinses, with tap water gently the residual miscellaneous tissue wash on the liver vessel cast moulds after corrosion is clean,
The liver vessel cast moulds that must be rinsed well.
7) it bleaches, the liver vessel cast moulds rinsed well is placed in the hydrogen peroxide solution that mass fraction is 8% and are floated
White 2 days, the liver vessel cast moulds after must bleaching.
8) it modifies, the liver vessel cast moulds after bleaching is rinsed well and dried, later remove vascular clot, dredge
It is close to beat branch, heat orthopedic, fixed morphologic localization, disconnected to draw glass glue and glue, it is good that finishing will reach permeability, big blood vessel, small
Vascular distribution is uniform, the vivid effect of color.
The toughness blood vessel casting sample of the present embodiment is prepared by the preparation method of the toughness blood vessel casting of the present embodiment.
Embodiment 3
The blood vessel casting agent of the present embodiment, including following component:
Chliorinated polyvinyl chloride 100kg, solvent 60kg, plasticizer 0.8kg, softening agent 6kg, fat-soluble pigment 1kg.
Solvent is ethyl acetate.
Plasticizer is dibutyl phthalate.
Softening agent is liquid polysulphide rubber.
Fat-soluble pigment is acrylic paints, and fat-soluble pigment includes red pigment and blue pigment.
The preparation method of the toughness blood vessel casting sample of the present embodiment, the preparation with the toughness blood vessel casting in embodiment 1
Method is identical.
The toughness blood vessel casting sample of the present embodiment is prepared by the preparation method of the toughness blood vessel casting of the present embodiment.
Embodiment 4
The blood vessel casting agent of the present embodiment, including following component:
Chliorinated polyvinyl chloride 100kg, solvent 30kg, plasticizer 0.3kg, softening agent 3kg, fat-soluble pigment 0.9kg.
Solvent is acetone.
Plasticizer is dihexyl adipate.
Softening agent is the mixture of liquid nitrile rubber and liquid polysulphide rubber, liquid nitrile rubber and liquid polysulphide rubber
Mass ratio be 5:1.
Fat-soluble pigment is acrylic paints, and fat-soluble pigment includes red pigment and blue pigment.
The preparation method of the toughness blood vessel casting sample of the present embodiment, the preparation with the toughness blood vessel casting in embodiment 2
Method is identical.
The toughness blood vessel casting sample of the present embodiment is prepared by the preparation method of the toughness blood vessel casting of the present embodiment.
Embodiment 5
The blood vessel casting agent of the present embodiment, including following component:
Vinylidene Chloride 100kg, solvent 55kg, plasticizer 0.6kg, softening agent 7kg, fat-soluble pigment 1.2kg.
Solvent is ethyl acetate.
Plasticizer is dibutyl phthalate.
Softening agent is liquid polysulphide rubber.
Fat-soluble pigment is acrylic paints.
The preparation method of the toughness blood vessel casting sample of the present embodiment, the preparation with the toughness blood vessel casting in embodiment 2
Method is identical.
The toughness blood vessel casting sample of the present embodiment is prepared by the preparation method of the toughness blood vessel casting of the present embodiment.
Embodiment 6
The blood vessel casting agent of the present embodiment, including following component:
Chliorinated polyvinyl chloride 100kg, solvent 50kg, plasticizer 0.4kg, softening agent 5kg, fat-soluble pigment 1.1kg.
Solvent is acetone.
Plasticizer is dihexyl adipate.
Softening agent is the mixture of liquid nitrile rubber and liquid polysulphide rubber, liquid nitrile rubber and liquid polysulphide rubber
Mass ratio be 3:1.
Fat-soluble pigment is acrylic paints, and fat-soluble pigment includes red pigment and blue pigment.
The preparation method of the toughness blood vessel casting sample of the present embodiment, the preparation with the toughness blood vessel casting in embodiment 1
Method is identical.
The toughness blood vessel casting sample of the present embodiment is prepared by the preparation method of the toughness blood vessel casting of the present embodiment.
Claims (10)
1. blood vessel casting agent, which is characterized in that the component including following mass fraction:
100 parts of mold material, 30~60 parts of solvent, 0.3~0.8 part of plasticizer, 3~7 parts of softening agent；
Mold material is one or more of chliorinated polyvinyl chloride, Vinylidene Chloride or polyvinyl chloride；
Softening agent is one or more of liquid nitrile rubber, liquid polysulphide rubber.
2. blood vessel casting agent according to claim 1, which is characterized in that the solvent is ethyl acetate or acetone.
3. blood vessel casting agent according to claim 1, which is characterized in that the plasticizer is dihexyl adipate or adjacent benzene
Dibutyl carboxylic acid.
4. blood vessel casting agent according to claim 1, which is characterized in that the mold material is chliorinated polyvinyl chloride.
5. blood vessel casting agent according to claim 1, which is characterized in that the softening agent is liquid nitrile rubber and liquid
The mass ratio of the mixture of polysulfide rubber, liquid nitrile rubber and liquid polysulphide rubber is 3:1~5:1.
6. blood vessel casting agent according to claim 1, which is characterized in that the blood vessel casting agent further includes 0.8~1.2 matter
The fat-soluble pigment of number is measured, fat-soluble pigment is acrylic paints.
7. using the method for blood vessel casting agent described in claim 1 preparation toughness blood vessel casting sample, which is characterized in that including
Following steps:
1) blood vessel casting agent is prepared, mold material, solvent, plasticizer, the softening agent of formula ratio are taken, is uniformly mixed to get blood vessel
Cast agent；
2) biological sample to after being intubated once is perfused in blood vessel casting agent prepared in step 1) by Perfused vessel cast agent
In, secondary pouring is carried out after being spaced 1~3h, the blood vessel casting sample after must being perfused；
3) corrode, the blood vessel casting sample in step 2) after perfusion stood into 12~14h so that blood vessel casting agent solidifies, postposition
Corrode 5~15d, the blood vessel casting sample after must corroding in hydrochloric acid；
4) it bleaches, the blood vessel casting sample after the corrosion in step 3) is rinsed well, be placed on 2~3d of bleaching in hydrogen peroxide,
Up to toughness blood vessel casting sample after finishing.
8. the method for preparation toughness blood vessel casting sample according to claim 7, which is characterized in that two described in step 2)
Secondary perfusion is supplement perfusion, and the number for supplementing perfusion is 2~3 times.
9. the method for preparation toughness blood vessel casting sample according to claim 7, which is characterized in that salt described in step 3)
The percent concentration of acid is 15~25%, and the mass concentration of hydrogen peroxide described in step 4) is 8~12%.
10. using the toughness blood vessel casting sample of blood vessel casting agent described in claim 1 preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811642232.1A CN109679258A (en) | 2018-12-29 | 2018-12-29 | Blood vessel casting agent, toughness blood vessel casting sample and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811642232.1A CN109679258A (en) | 2018-12-29 | 2018-12-29 | Blood vessel casting agent, toughness blood vessel casting sample and preparation method thereof |
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| CN109679258A true CN109679258A (en) | 2019-04-26 |
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| CN201811642232.1A Pending CN109679258A (en) | 2018-12-29 | 2018-12-29 | Blood vessel casting agent, toughness blood vessel casting sample and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110859999A (en) * | 2019-10-11 | 2020-03-06 | 南方医科大学 | Construction method of three-dimensional vascular network hydrogel |
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|---|---|---|---|---|
| CN101003663A (en) * | 2006-12-30 | 2007-07-25 | 南京师范大学 | Cast agent for blood vessel of no-solvent type epoxy resin |
| CN104497485A (en) * | 2014-12-18 | 2015-04-08 | 黄海龙 | Body sample angiographic filler and preparation method thereof |
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| CN110859999A (en) * | 2019-10-11 | 2020-03-06 | 南方医科大学 | Construction method of three-dimensional vascular network hydrogel |
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