CN109652335A - A kind of preparation method of feeding solid lactic acid bacterium high activity microbial inoculum - Google Patents
A kind of preparation method of feeding solid lactic acid bacterium high activity microbial inoculum Download PDFInfo
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- CN109652335A CN109652335A CN201910051895.4A CN201910051895A CN109652335A CN 109652335 A CN109652335 A CN 109652335A CN 201910051895 A CN201910051895 A CN 201910051895A CN 109652335 A CN109652335 A CN 109652335A
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- lactic acid
- parts
- acid bacteria
- microbial inoculum
- preparation
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 title claims abstract description 168
- 241000894006 Bacteria Species 0.000 title claims abstract description 118
- 239000004310 lactic acid Substances 0.000 title claims abstract description 84
- 235000014655 lactic acid Nutrition 0.000 title claims abstract description 84
- 239000007787 solid Substances 0.000 title claims abstract description 45
- 238000002360 preparation method Methods 0.000 title claims abstract description 33
- 230000000694 effects Effects 0.000 title claims abstract description 20
- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 19
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 47
- 238000000855 fermentation Methods 0.000 claims abstract description 18
- 230000004151 fermentation Effects 0.000 claims abstract description 18
- 239000000843 powder Substances 0.000 claims abstract description 12
- 235000013406 prebiotics Nutrition 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 33
- 239000000203 mixture Substances 0.000 claims description 29
- 238000009472 formulation Methods 0.000 claims description 26
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 19
- 238000003756 stirring Methods 0.000 claims description 15
- 239000000284 extract Substances 0.000 claims description 13
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims description 10
- 235000020183 skimmed milk Nutrition 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- 239000000419 plant extract Substances 0.000 claims description 9
- 229920002472 Starch Polymers 0.000 claims description 8
- 235000011187 glycerol Nutrition 0.000 claims description 8
- 239000002253 acid Substances 0.000 claims description 6
- 235000013305 food Nutrition 0.000 claims description 6
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- 108010024636 Glutathione Proteins 0.000 claims description 5
- 229920000294 Resistant starch Polymers 0.000 claims description 5
- 229930006000 Sucrose Natural products 0.000 claims description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 5
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 5
- 239000005862 Whey Substances 0.000 claims description 5
- 102000007544 Whey Proteins Human genes 0.000 claims description 5
- 108010046377 Whey Proteins Proteins 0.000 claims description 5
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 5
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 5
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical compound O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 claims description 5
- 239000006185 dispersion Substances 0.000 claims description 5
- 239000000706 filtrate Substances 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 239000012467 final product Substances 0.000 claims description 5
- 229960003180 glutathione Drugs 0.000 claims description 5
- 229910052901 montmorillonite Inorganic materials 0.000 claims description 5
- 235000021254 resistant starch Nutrition 0.000 claims description 5
- 235000019698 starch Nutrition 0.000 claims description 5
- 239000008107 starch Substances 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 239000005720 sucrose Substances 0.000 claims description 5
- 239000011248 coating agent Substances 0.000 claims description 4
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 229920001282 polysaccharide Polymers 0.000 claims description 4
- 239000005017 polysaccharide Substances 0.000 claims description 4
- 238000002390 rotary evaporation Methods 0.000 claims description 4
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 3
- 241000186016 Bifidobacterium bifidum Species 0.000 claims description 3
- 229920002261 Corn starch Polymers 0.000 claims description 3
- 241001147746 Lactobacillus delbrueckii subsp. lactis Species 0.000 claims description 3
- 229920000168 Microcrystalline cellulose Polymers 0.000 claims description 3
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims description 3
- 229940002008 bifidobacterium bifidum Drugs 0.000 claims description 3
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 3
- 239000008120 corn starch Substances 0.000 claims description 3
- 229940099112 cornstarch Drugs 0.000 claims description 3
- 239000006071 cream Substances 0.000 claims description 3
- 238000005469 granulation Methods 0.000 claims description 3
- 230000003179 granulation Effects 0.000 claims description 3
- 235000019813 microcrystalline cellulose Nutrition 0.000 claims description 3
- 239000008108 microcrystalline cellulose Substances 0.000 claims description 3
- 229940016286 microcrystalline cellulose Drugs 0.000 claims description 3
- 229920001592 potato starch Polymers 0.000 claims description 3
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims description 3
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims description 3
- 229940100445 wheat starch Drugs 0.000 claims description 3
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 2
- 241000194032 Enterococcus faecalis Species 0.000 claims description 2
- 241000194031 Enterococcus faecium Species 0.000 claims description 2
- 241000208688 Eucommia Species 0.000 claims description 2
- 240000004670 Glycyrrhiza echinata Species 0.000 claims description 2
- 235000001453 Glycyrrhiza echinata Nutrition 0.000 claims description 2
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 claims description 2
- 235000017382 Glycyrrhiza lepidota Nutrition 0.000 claims description 2
- 244000199866 Lactobacillus casei Species 0.000 claims description 2
- 235000013958 Lactobacillus casei Nutrition 0.000 claims description 2
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 2
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 2
- 229920002774 Maltodextrin Polymers 0.000 claims description 2
- 239000005913 Maltodextrin Substances 0.000 claims description 2
- 241000191998 Pediococcus acidilactici Species 0.000 claims description 2
- 241000191996 Pediococcus pentosaceus Species 0.000 claims description 2
- 239000011575 calcium Substances 0.000 claims description 2
- 229910052791 calcium Inorganic materials 0.000 claims description 2
- 229940032049 enterococcus faecalis Drugs 0.000 claims description 2
- 229940017800 lactobacillus casei Drugs 0.000 claims description 2
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 2
- 229940010454 licorice Drugs 0.000 claims description 2
- 229940035034 maltodextrin Drugs 0.000 claims description 2
- 229940116317 potato starch Drugs 0.000 claims description 2
- 239000000661 sodium alginate Substances 0.000 claims description 2
- 235000010413 sodium alginate Nutrition 0.000 claims description 2
- 229940005550 sodium alginate Drugs 0.000 claims description 2
- 238000001694 spray drying Methods 0.000 claims description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims 1
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims 1
- 239000000460 chlorine Substances 0.000 claims 1
- 229910052801 chlorine Inorganic materials 0.000 claims 1
- 238000000605 extraction Methods 0.000 claims 1
- 150000004676 glycans Chemical class 0.000 claims 1
- 241000186660 Lactobacillus Species 0.000 abstract description 5
- 229940039696 lactobacillus Drugs 0.000 abstract description 5
- 210000000941 bile Anatomy 0.000 abstract description 4
- 210000000936 intestine Anatomy 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 4
- 230000000975 bioactive effect Effects 0.000 abstract description 3
- 239000006041 probiotic Substances 0.000 abstract description 3
- 235000018291 probiotics Nutrition 0.000 abstract description 3
- 210000002784 stomach Anatomy 0.000 abstract description 3
- 238000005457 optimization Methods 0.000 abstract description 2
- 230000000529 probiotic effect Effects 0.000 abstract description 2
- 238000004088 simulation Methods 0.000 abstract description 2
- 230000035899 viability Effects 0.000 abstract 1
- 238000002156 mixing Methods 0.000 description 7
- 239000000047 product Substances 0.000 description 5
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 3
- 210000004209 hair Anatomy 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 150000004804 polysaccharides Chemical class 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 240000001046 Lactobacillus acidophilus Species 0.000 description 2
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 229940099352 cholate Drugs 0.000 description 2
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000004925 Acrylic resin Substances 0.000 description 1
- 229920000178 Acrylic resin Polymers 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 235000003717 Boswellia sacra Nutrition 0.000 description 1
- 240000007551 Boswellia serrata Species 0.000 description 1
- 235000012035 Boswellia serrata Nutrition 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 1
- 239000004863 Frankincense Substances 0.000 description 1
- 208000018522 Gastrointestinal disease Diseases 0.000 description 1
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 1
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 150000004781 alginic acids Chemical class 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000003266 anti-allergic effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000007596 consolidation process Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 235000003969 glutathione Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000000266 injurious effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- -1 iron ion Chemical class 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/157—Lactis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of preparation methods of feeding solid lactic acid bacterium high activity microbial inoculum.The preparation process of the lactic bacteria activity microbial inoculum includes the fermentation of probiotic lactobacillus live bacteria agent, the basic recipe of lactic acid bacteria solid-state microbial inoculum and preparation and powder prilling comprising formula.The present invention improves its prebiotic function while guaranteeing lactic acid bacteria high viability by adding the optimization of various bioactive components and basic recipe and protection formula in the fermentation process of viable lactic acid bacteria agent.Lactic acid bacteria is acidproof in lactic bacteria activity microbial inoculum prepared by the present invention, bile tolerance ability improves, and survival rate is high in simulation animal intestines and stomach liquid, has good prebiotic healthcare function.
Description
Technical field
The invention belongs to bioengineering fields, and in particular to a kind of preparation side of feeding solid lactic acid bacterium high activity microbial inoculum
Method.
Background technique
Active lactic acid bacteria agent have promote gastrointestinal disturbances absorb, adjust intestinal microecology balance, improve immunity of organisms,
The functions such as disease-prevention health.Meanwhile lactobacillus preparation is because its edible natural uses, has no toxic side effect, safe and reliable and easily extensive hair
The advantages such as ferment are widely used in the industries such as health care of food, livestock feed, aquaculture, microbial fermentation and environmental protection.So
And presently commercially available lactic acid bacteria is mostly liquid bacterial agent, dosage form is single and storage life is relatively short, and solid lactic acid bacteria preparation is not only
Delicate mouthfeel, and convenient for storage and transport.Therefore, the exploitation and preparation of lactic acid bacteria solid active microbial inoculum have a vast market
Potentiality.
Currently, the fermentation Study on Preparation in relation to lactic acid bacteria solid active microbial inoculum is less, formula needs optimization and upgrading.Specially
Sharp 1369553 A of CN discloses a kind of method for preparing solid active lactobacillus preparation, which adds solid starch matter carbon source
With a variety of nitrogen sources, using saccharification aspergillus starch-splitting, and solid formulation is prepared as by low temperature drying.The invention technique letter
Single easy, product is convenient for saving, but zymophyte work is not high, and product nutrient prebiotic substance is single.1400304 A of patent CN
Disclose a kind of liquid-consolidation combined fermentation technology of probiotics, the solid fermentation link of the invention in anaerobism polybag into
Row, solid matrix ingredient is simple, fermentation-scale is limited.
The preparation process of lactic acid bacteria high activity microbial inoculum of the present invention includes the fermentation of probiotic lactobacillus live bacteria agent, lactic acid
The preparation of bacterium solid-state microbial inoculum basic recipe and protection formula and later period powder granulation process.The present invention is in viable lactic acid bacteria agent
Various bioactive components are added in fermentation process, and are formulated as the cream rich in a variety of nutrition prebiotic components and thallus Protective substances
Sour bacteria preparation eventually passes through drying process and obtains milky white granules shape lactic acid bacteria product.The preparation side of the lactic bacteria activity microbial inoculum
Method is economic and environment-friendly, and simple production process is efficient, and production cost is low；Product free from extraneous odour, mouthfeel is soft, there is natural fermented frankincense taste；
Its acid-fast ability improves 50% or more, bile tolerance ability and is enhanced about more than once, and animal intestines and stomach liquid survival rate is high；Cream with higher
Sour bacterium survival rate and the prebiotic function of plurality kinds of health care.
The advantage of the invention is that quantitatively adding montmorillonite, trehalose, Pu Lu in the fermentation process of viable lactic acid bacteria agent
The various bioactive components such as blue polysaccharide, glutathione, resistant starch, glycerol, different VC acid, it is living effectively improving lactic acid bacteria agent
While property, the nutritional ingredient and healthcare function of product are enhanced.It adsorbs and fixes for example, montmorillonite has, inactivate the micro- life of cause of disease
Object, and absorption, the effect for removing toxin and injurious factor are that a kind of have emulsification, thickening, suspending, adsorption function and well
Medical value bioactivity auxiliary material.Resistant starch can remarkably promote lactobacter growth, assist enteron aisle to iron ion and calcium from
Son absorption, and it is from a wealth of sources, preparation process is simple, in good taste.Glutathione then has antiallergy, antitumor, anti-aging
It is a kind of important antioxidant with the functions such as strengthen immunity.
Summary of the invention
A kind of feeding solid lactic acid bacterium high activity microbial inoculum prepared by the present invention, specifically includes viable lactic acid bacteria agent and lactic acid bacteria
The fermentation of solid formulation, basic recipe, the preparation of protection formula and the powder of solid formulation of lactic acid bacteria solid formulation are granulated
Technique.
Firstly, fermentation prepares lactic acid live bacteria agent, fermentation strain source includes enterococcus faecium, enterococcus faecalis, lactic acid intestines ball
Bacterium, bifidobacterium bifidum, lactobacillus acidophilus, Lactobacillus casei, lactobacillus lactis, lactobacillus plantarum, Pediococcus acidilactici, rhamnose
One or more of lactobacillus, Pediococcus pentosaceus or bacillus bulgaricus.
Wherein, lactobacillus-fermented culture medium adds skimmed milk power 10-15%(W/V in parts by weight) and it is two kinds following
Or two kinds or more of mixture:
Resistant starch 1-3%
Trehalose 3-5%
Pulullan polysaccharide 1-3%
Different VC acid 0.1-0.5%
Glutathione 0.2-0.5 mM
Montmorillonite 0.1-1.0%
Glycerol 0.5-2.5%
The specific zymotechnique of lactic acid live bacteria agent are as follows: 105-110 DEG C of sterilizing 10-15 min of culture medium, after being cooled to room temperature,
Lactobacillus-fermented strain is accessed in the ratio of 1~3 %.In 30 DEG C of -39 DEG C of ferment at constant temperature 5-8 h after inoculation, until skimmed milk power is newborn
Solidification, fermentation ends when pH is 4.0-4.5.150-200 rpm stirring is cooled to room temperature, and obtains viable lactic acid bacteria agent, has
Imitate viable count >=2.0 × 109cfu/mL。
Viable lactic acid bacteria agent is formulated as basic recipe and protection formula solid formulation respectively, preparation process is as described below.
Above-mentioned viable lactic acid bacteria agent and each component are mixed, water is added while stirring.In parts by weight, each component
Are as follows: prebiotic factor 3-10 parts, vigorous agent 3-10 parts of knot, 20.5-55 parts of coating agent, 34-67 parts of starch and 25-70 parts of water.It is sufficiently mixed
Cyclone drying, as basic recipe solid formulation under the conditions of 20-45 DEG C after even.
Wherein, the above-mentioned prebiotic factor is one or both of xylo-oligosaccharide or oligofructose；Vigorous knot agent is that shuttle methyl is fine
Tie up plain one or both of sodium or microcrystalline cellulose；Coating agent is in polyacrylic resinⅡ, sodium alginate or calcium chloride
It is one or more of；Starch is one or more of cornstarch, wheat starch or potato starch.
It takes viable lactic acid bacteria agent 3500-4000 rpm to be centrifuged 8-10 min, collects bacterium mud.In the bacterium mud and protection formula
Various component mixing, are then added water, dispersion stirs evenly, and obtains creamy formulations.In parts by weight, every 1-5 parts of bacterium mud mixes other groups
Point: 0.1-2.0 parts of food grade glycerin, 0.4-2.0 parts of sucrose, 0.3-5.0 parts of maltodextrin, 0.1-3.0 parts of whey powder, plant mention
Take 0.06-1.5 parts of object, 0.5-7.0 parts of water.
Wherein, above-mentioned plant extracts is one of eucommia leaf extract, dried orange peel extracts or licorice or several
Kind.Preparation method are as follows: take the object to be extracted of 1-10 parts by weight, 40-55 DEG C of drying to water content is lower than 10%, is crushed to 60-80
Mesh, dehydrated alcohol extract 1-3 h.With 100-200 mesh net filtration and filtrate is collected, 40-55 DEG C of rotary evaporation obtains extract,
The water that 10-200 parts by weight are added sufficiently dissolves to obtain the final product.
The feeding high-activity lactic acid bacteria preparation of gained protection formula carries out powder granulation using spray drying, and actual conditions are
Feed rate 2-5 mL/min, intake 40-50 m3/ h, 55-80 DEG C of leaving air temp.
The water content of above-mentioned basic recipe and protection formula lactic acid bacteria solid formulation is 3.0-5.0 %, the effective bacterium of lactic acid bacteria
Viable count >=1.0 × 1012cfu/g。
Detailed description of the invention
Fig. 1 is different lactic acid bacteria agent acid resistance results
Fig. 2 is different lactic acid bacteria agent Pig cholate tolerability results
Specific embodiment
The present invention will be further explained combined with specific embodiments below, so that those skilled in the art knows more about
The present invention, but be not intended to limit the present invention.
Embodiment 1
Firstly, preparing 12%(W/V) skimmed milk power, the trehalose and 0.3 mM glutathione of 3 %, 110 DEG C of sterilizings 10 are added
min.After being cooled to room temperature, bifidobacterium bifidum, fermenting lactic acid bacterium live bacteria agent are accessed in the ratio of 3 %.35 DEG C of constant temperature hairs
6 h of ferment, until skimmed milk power curdling is solid, fermentation ends when pH is 4.5.It by 150 rpm stirring, is cooled to room temperature, obtains lactic acid
Bacterium live bacteria agent, living bacteria count are 2.0 × 109Cfu/mL.
Then, above-mentioned viable lactic acid bacteria agent is formulated as basic recipe solid formulation.By viable lactic acid bacteria agent and each component
It is mixed, water is added while stirring.In parts by weight, each component are as follows: 5 parts of xylo-oligosaccharide, 4 parts of shuttle sodium carboxymethylcellulose pyce, sea
25 parts of mosanom, 50 parts of cornstarch and 40 parts of water.Cyclone drying, as basic recipe solid-state under the conditions of 30 DEG C after mixing well
Preparation.4.5 % of gained basic recipe lactic acid bacteria solid formulation water content, lactic acid bacteria living bacteria count 1.08 × 1012cfu/g。
At the same time, above-mentioned viable lactic acid bacteria agent is formulated as protection formula solid formulation.Take viable lactic acid bacteria agent 3500
Rpm is centrifuged 10 min, collects bacterium mud.In parts by weight, 2 parts of bacterium muds and 0.1 part of food grade glycerin, 0.4 part of sucrose, malt is taken to paste
0.4 part of essence, 0.8 part of whey powder, 1.0 parts of plant extracts mixing, are then added 2.5 parts of water, dispersion stirs evenly, and obtains protection laboratory milk
Shape preparation.
Wherein, extract plant extracts the preparation method comprises the following steps: by the folium cortex eucommiae of 2 parts by weight and the dried orange peel of 3 parts by weight, 45 DEG C it is dry
It is dry to be lower than 10% to water content, 60 mesh are crushed to, dehydrated alcohol extracts 2 h.With 200 mesh net filtrations and filtrate is collected, 45 DEG C of rotations
Turn evaporation and obtain extract, with the water that 50 parts by weight are added, sufficiently dissolve to obtain the final product.
The feeding high-activity lactic acid bacteria preparation of gained protection formula is spray-dried, and actual conditions are feed rate 3
ML/min, 45 m of intake3/ h, 60 DEG C of leaving air temp.Gained protection formula 4.2 % of lactic acid bacteria solid formulation water content, lactic acid
Bacterium living bacteria count 1.17 × 1012cfu/g。
Embodiment 2
Firstly, preparing 15%(W/V) skimmed milk power, 2% pulullan polysaccharide, 2% resistant starch and the 0.3 different VC of % mM is added
Acid, 110 DEG C of 12 min of sterilizing.After being cooled to room temperature, it is inoculated in the ratio of 3 % lactobacillus lactis, 2 % bacillus bulgaricus, hair
Ferment produces viable lactic acid bacteria agent.39 DEG C of 5 h of ferment at constant temperature, until skimmed milk power curdling is solid, fermentation ends when pH is 4.0.By
200 rpm stirring is cooled to room temperature, and obtains viable lactic acid bacteria agent, and living bacteria count is 3.6 × 109Cfu/mL.
Then, above-mentioned viable lactic acid bacteria agent is formulated as basic recipe solid formulation.By viable lactic acid bacteria agent and each component
It is mixed, water is added while stirring.In parts by weight, each component are as follows: 10 parts of oligofructose, 8 parts of microcrystalline cellulose, alginic acid
10 parts of sodium, 10 parts of calcium chloride, 50 parts of potato starch and 45 parts of water.Cyclone drying, as base under the conditions of 45 DEG C after mixing well
This formula solid formulation.3.2 % of gained basic recipe lactic acid bacteria solid formulation water content, lactic acid bacteria living bacteria count 1.45 ×
1012cfu/g。
At the same time, above-mentioned viable lactic acid bacteria agent is formulated as protection formula solid formulation.Take viable lactic acid bacteria agent 4000
Rpm is centrifuged 8 min, collects bacterium mud.In parts by weight, 5 parts of bacterium muds and 1.0 parts of food grade glycerin, 0.9 part of sucrose, malt is taken to paste
2.5 parts of essence, 1.5 parts of whey powder, 1.2 parts of plant extracts mixing, are then added 5.8 parts of water, dispersion stirs evenly, and obtains creamy formulations.
Wherein, plant is extracted the preparation method comprises the following steps: the Radix Glycyrrhizae of the folium cortex eucommiae of 3 parts by weight and 2 parts by weight is dried extremely at 55 DEG C
Moisture content 8%, is crushed to 80 mesh, and dehydrated alcohol extracts 3 h.With 200 mesh net filtrations and filtrate is collected, 55 DEG C of rotary evaporations
It obtains extract, with the water that 50 parts by weight are added, sufficiently dissolves to obtain the final product.
The feeding high-activity lactic acid bacteria preparation of gained protection formula is spray-dried, and actual conditions are feed rate
2.0 mL/min, 50 m of intake3/ h, 60 DEG C of leaving air temp.Gained protection formula 3.6 % of lactic acid bacteria solid formulation water content,
Lactic acid bacteria living bacteria count 1.60 × 1012cfu/g。
Embodiment 3
Firstly, prepare 15%(W/V) skimmed milk power, 3.5%(W/V is added) trehalose and 0.5 % montmorillonite, 108 DEG C of sterilizings
12 min.After being cooled to room temperature, lactobacillus acidophilus, fermenting lactic acid bacterium live bacteria agent are accessed in the ratio of 3 %.In 37 DEG C of conditions
Lower 6 h of ferment at constant temperature, until skimmed milk power curdling is solid, fermentation ends when pH is 4.3.By 180 rpm stirring, it is cooled to room temperature,
Viable lactic acid bacteria agent is obtained, living bacteria count is 2.5 × 109Cfu/mL.
Then, above-mentioned viable lactic acid bacteria agent is formulated as basic recipe solid formulation.By viable lactic acid bacteria agent and each component
It is mixed, water is added while stirring.In parts by weight, each component are as follows: 5 parts of xylo-oligosaccharide, gathers 5 parts of shuttle sodium carboxymethylcellulose pyce
II 35 parts of acrylic resin, 50 parts of wheat starch and 40 parts of water.Cyclone drying under the conditions of 40 DEG C after mixing well, as substantially
It is formulated solid formulation.4.1 % of basic recipe lactic acid bacteria solid formulation water content, lactic acid bacteria living bacteria count 1.25 ×
1012cfu/g。
At the same time, above-mentioned viable lactic acid bacteria agent is formulated as protection formula solid formulation.Take viable lactic acid bacteria agent 3800
Rpm is centrifuged 8 min, collects bacterium mud.In parts by weight, 3 parts of bacterium muds and 1.0 parts of food grade glycerin, 1.0 parts of sucrose, malt is taken to paste
3.0 parts of essence, 2.0 parts of whey powder, 1.0 parts of plant extracts mixing, are then added 5.0 parts of water, dispersion stirs evenly, and obtains protection laboratory milk
Shape preparation.
Wherein, plant extracts the preparation method comprises the following steps: the folium cortex eucommiae of 3 parts by weight dry is lower than to water content at 50 DEG C
9%, 80 mesh are crushed to, dehydrated alcohol extracts 3 h.With 200 mesh net filtrations and filtrate is collected, 50 DEG C of rotary evaporations are extracted
Object is sufficiently dissolved with the water that 20 parts by weight are added to obtain the final product.
The feeding high-activity lactic acid bacteria preparation of gained protection formula is spray-dried, and actual conditions are feed rate 3
ML/min, 45 m of intake3/ h, 65 DEG C of leaving air temp.Gained protection formula 3.5 % of lactic acid bacteria solid formulation water content, lactic acid
Bacterium living bacteria count 1.33 × 1012cfu/g。
Reference examples 1 are common commercially available lactic acid bacteria agent.
Each embodiment and comparative example acid resistance analyze result such as Fig. 1, and Pig cholate tolerance analyzes result such as Fig. 2.
As seen from table, the acidproof and bile tolerance ability of the lactic acid bacteria agent of each embodiment is respectively increased compared with comparative example
50% or more and one times or more.Therefore, the acidproof and bile tolerance ability of the lactic acid bacteria agent of the method preparation is equal according to the present invention
It greatly improves, microbial inoculum survival rate in simulation animal intestines and stomach liquid is higher.
Claims (7)
1. specifically including viable lactic acid bacteria agent the present invention relates to a kind of preparation method of feeding solid lactic acid bacterium high activity microbial inoculum
Fermentation, the preparation of the basic recipe and protection formula of lactic acid bacteria solid formulation and the powder prilling of later period solid formulation.
2. as described in claim 1, it is characterised in that the fermenting microbe that the lactic acid bacteria solid formulation uses includes but unlimited
In one or more of following lactic acid bacteria: enterococcus faecium, enterococcus faecalis, lactoenterococcus, bifidobacterium bifidum, acidophilus cream bar
Bacterium, Lactobacillus casei, lactobacillus lactis, lactobacillus plantarum, Pediococcus acidilactici, Lactobacillus rhamnosus, Pediococcus pentosaceus or guarantor add
Leah bacillus.
3. as described in claim 1, it is characterised in that the zymotechnique of the viable lactic acid bacteria agent is as follows:
105-110 DEG C of sterilizing 10-15 min of culture medium after being cooled to room temperature, accesses lactobacillus-fermented bacterium in the ratio of 1~3 %
30 DEG C of -39 DEG C of ferment at constant temperature 5-8 h are solid to skimmed milk power curdling after kind of so be inoculated with, when pH 4.0-4.5 fermentation ends stir,
It is cooled to room temperature, obtains viable lactic acid bacteria agent
Wherein, lactobacillus-fermented culture medium adds 10-15%(W/V in parts by weight) skimmed milk power and two kinds or two following
Kind or more mixture:
Resistant starch 1-3%
Trehalose 3-5%
Pulullan polysaccharide 1-3%
Different VC acid 0.1-0.5%
Glutathione 0.2-0.5 mM
Montmorillonite 0.1-1.0%
Glycerol 0.5-2.5%
As described in claim 1, it is characterised in that prepared by the basic recipe of the feeding solid lactic acid bacterium high activity microbial inoculum
Journey is as follows:
It takes viable lactic acid bacteria agent obtained and other each components in claim 3 to be mixed, water is added while stirring with weight
Part meter, other each components are as follows: prebiotic factor 3-10 parts, vigorous agent 3-10 parts of knot, 20.5-55 parts of coating agent, 34-67 parts of starch and water
25-70 parts, mix well wherein, the above-mentioned prebiotic factor is one or both of xylo-oligosaccharide or oligofructose；Suddenly tying agent is
One or both of shuttle sodium carboxymethylcellulose pyce or microcrystalline cellulose；Coating agent is polyacrylic resinⅡ, sodium alginate or chlorine
Change one or more of calcium；Starch is one or more of cornstarch, wheat starch or potato starch.
4. as described in claim 1, it is characterised in that the powder of the feeding solid lactic acid bacterium high activity microbial inoculum basic recipe
Prilling is cyclone drying under the conditions of 20-45 DEG C.
5. as described in claim 1, it is characterised in that the protection of the feeding solid lactic acid bacterium high activity microbial inoculum is formulated preparation
Process is as follows:
It takes viable lactic acid bacteria agent obtained in claim 3 to be centrifuged and collect bacterium mud, is mixed with the various components in protection formula,
Then water is added, dispersion stirs evenly, and bacterium solution is made in parts by weight, and every 1-5 parts of bacterium mud mixes other components are as follows: food grade glycerin
0.1-2.0 parts, 0.4-2.0 parts of sucrose, 0.3-5.0 parts of maltodextrin, 0.1-3.0 parts of whey powder, plant extracts 0.06-1.5
Wherein, above-mentioned plant extracts is one in eucommia leaf extract, dried orange peel extracts or licorice by 0.5-7.0 parts of part, water
Kind is several.
6. the extracting method of plant extracts are as follows: take the object to be extracted of 1-10 parts by weight, 40-55 DEG C of drying to water content is lower than
10%, it is crushed to 60-80 mesh, filtrate is collected by filtration in dehydrated alcohol extraction 1-3 h., and 40-55 DEG C of rotary evaporation obtains extract, uses
The water of 10-200 parts by weight sufficiently dissolves to obtain the final product.
7. as described in claim 1, it is characterised in that the protection of the feeding solid lactic acid bacterium high activity microbial inoculum, which is formulated, to be used
Spray drying carries out powder granulation, concrete technology feed rate 2-5 mL/min, intake 40-50 m3/ h, leaving air temp 55-
80℃。
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