CN117987270A - Application of edible fungus polysaccharide in spray drying of pediococcus acidilactici - Google Patents
Application of edible fungus polysaccharide in spray drying of pediococcus acidilactici Download PDFInfo
- Publication number
- CN117987270A CN117987270A CN202410308819.8A CN202410308819A CN117987270A CN 117987270 A CN117987270 A CN 117987270A CN 202410308819 A CN202410308819 A CN 202410308819A CN 117987270 A CN117987270 A CN 117987270A
- Authority
- CN
- China
- Prior art keywords
- polysaccharide
- bacterial
- spray drying
- edible fungus
- pediococcus acidilactici
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- 241000191998 Pediococcus acidilactici Species 0.000 title claims abstract description 34
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
- A23K30/20—Dehydration
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/16—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by heating loose unpacked materials
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3562—Sugars; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/40—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by drying or kilning; Subsequent reconstitution
- A23L3/46—Spray-drying
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- A—HUMAN NECESSITIES
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- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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Abstract
The invention discloses application of edible fungus polysaccharide in Pediococcus acidilactici spray drying, and belongs to the technical field of probiotics spray drying. The invention discovers that the edible fungus polysaccharide protective agent can obviously improve the viable count of Pediococcus acidilactici in the spray drying process, and the viable count of Pediococcus acidilactici can reach more than 1.0X10 12 cfu/g by taking Cordyceps militaris polysaccharide, hericium erinaceus polysaccharide, tremella polysaccharide, auricularia polysaccharide and Agaricus blazei polysaccharide as the spray drying protective agent. Meanwhile, the production process is economical and convenient, continuous production can be realized, the flavor and nutrition of the fungus powder are enriched by adding the edible fungus polysaccharide, and the application potential of the pediococcus acidilactici in the probiotic market is widened.
Description
Technical Field
The invention relates to the technical field of probiotics spray drying, in particular to application of edible fungus polysaccharide in Pediococcus acidilactici spray drying.
Background
Pediococcus acidilactici (Pediococcus acidilactici) found in vegetables and cow's milk, which is not pathogenic to plants or animals, is Pediococcus (Pediococcus) belonging to the order Lactobacillus (bacili) of the order Lactobacillus (Lactobacillales) of the order Leuconostoc (Firmicutes) and the family Ballobacteriaceae (Aerococcaceae). The bacterial cells are in a sphere shape, are not prolonged, and have a diameter of 1.2-2.0 mu m. Gram-positive staining, no motility, no formation of spores, no pigment production, and facultative anaerobic growth. The colony surface is smooth and milky white, and the diameter is generally 1.0-2.5 mm. Has the functions of adjusting intestinal flora, preventing and treating intestinal diseases, improving the ecological environment of animals in and out, enhancing immunity, improving feed conversion rate and the like, and is now used in the industries of food, animal husbandry, aquaculture and the like.
The spray drying has the advantages of simple equipment and instrument, low cost, high efficiency, strong continuity and the like, and is suitable for large-scale production, transportation and storage of probiotics. However, in the spray drying process, the probiotic bacteria and the high-temperature air are fully contacted to cause damage to the bacteria, so that the viable count of the bacterial powder is seriously affected. Therefore, the reduction of viable count is a major problem in the preparation of bacterial powders by spray drying.
For many years, lactobacillus preparations have been studied at home and abroad, and lactobacillus is widely studied at present. And the survival rate of lactic acid bacteria in the bacterial powder prepared by a spray drying method can be effectively improved by adding a proper protective agent. Corcoran et al studied the effect of different wall materials on the survival rate of probiotics in the spray drying process and found that after combining skimmed milk with dextran or inulin, the survival rate of probiotics was significantly improved compared with single wall material. The Shore Huai Qiu et al optimize Bacillus subtilis Prob1822 to obtain the compound heat resistant protectant formulation: trehalose 9.0%, sucrose 5.0% and skimmed milk powder 6.8%, and the survival rate of the thalli reaches 95.24% +/-0.84% under the protection of the compound heat-resistant protective agent. Liu Huan and the like are used for preparing lactobacillus plantarum microcapsules by a spray drying method, low-melting-point fat is mixed into a wall material, so that the low-melting-point fat in the prepared microcapsules is dispersed around probiotics, and the heat damage of the spray drying to the thalli is reduced by absorbing heat, so that the number of living bacteria in the microcapsules is increased. In addition, the strain may be subjected to heat shock treatment before spray drying to change the heat resistance of the strain. The heat shock treatment is also called heat stress, and when the probiotics are placed in an environment with the temperature higher than the optimal growth temperature, the probiotics can generate a plurality of chaperones and proteases, and damaged cells are repaired, so that the tolerance of the bacteria to the high-temperature environment is enhanced. The heat resistance of probiotics L.caseiBD-II and the influence of different heat shock conditions on the heat resistance of the probiotics L.caseiBD-II are researched by the quartz and the like, and the optimal heat shock condition of the probiotics L.caseiBD-II is determined through experiments; the bacterial liquid after 7h of culture is subjected to heat shock treatment for 45min at 50 ℃, the bacterial survival rate is 0.146%, and compared with a test group without heat shock treatment, the bacterial survival rate is improved by 2.11 times; zhang Shumeng and the like research the influence of different heat shock treatment temperatures on the heat resistance of lactobacillus acidophilus, and the research shows that the optimal heat shock condition of lactobacillus acidophilus is 45 ℃ for 30min, and under the condition, after the heat treatment in a water bath at 80 ℃ for 5min, the number of viable bacteria is increased by one order of magnitude compared with the number of viable bacteria directly subjected to the heat treatment without the heat shock treatment; hu Liuliu and the like optimize the heat shock condition of lactobacillus casei, the heat resistance of the thallus is obviously improved after heat shock is carried out for 30min at 50 ℃, the viable count of the thallus can reach 6.55 xL 0 6 cfu/mL after heat treatment is carried out for 5min at 80 ℃, and the bacterial activity is obviously improved by one order of magnitude compared with a test group directly carrying out heat treatment at 80 ℃ without heat shock treatment.
Therefore, it is very important to find a suitable Pediococcus acidilactici spray drying protective agent and heat shock conditions, and data support is provided for the application and industrial production of Pediococcus acidilactici in the probiotic market.
Disclosure of Invention
The invention aims to overcome the defects and shortcomings of the prior art and provides application of edible fungus polysaccharide in spray drying of Pediococcus acidilactici. According to the invention, the heat resistance of the pediococcus acidilactici in the spray drying process can be improved by adding the edible fungus polysaccharide, so that the viable count of the fungus powder is improved.
The aim of the invention is achieved by the following technical scheme:
The edible fungus polysaccharide is any one of Cordyceps militaris polysaccharide, hericium erinaceus polysaccharide, tremella polysaccharide, auricularia polysaccharide and Agaricus blazei polysaccharide.
A spray drying protective agent for Pediococcus acidilactici contains edible fungus polysaccharide, wherein the edible fungus polysaccharide is any one of Cordyceps militaris polysaccharide, hericium erinaceus polysaccharide, tremella polysaccharide, auricularia polysaccharide and Agaricus blazei polysaccharide.
The application of the spray drying protective agent in the spray drying of pediococcus acidilactici.
Further, the application is in improving the number of live bacteria of the Pediococcus acidilactici spray drying.
Further, the edible fungus polysaccharide is an edible fungus polysaccharide aqueous solution with the concentration of 10+/-1% (w/v, g/mL).
Further, the application is as follows: preparing bacterial suspension, carrying out heat shock treatment on the bacterial suspension, centrifuging to obtain bacterial mud, and mixing the bacterial mud with an edible fungus polysaccharide aqueous solution according to a mass ratio of 1:3 to 7, preferably 1:5, fully mixing, and then carrying out spray drying, wherein the parameters of the spray drying are as follows: the air inlet temperature is 90+/-2 ℃, the air outlet temperature is controlled at 60 ℃ -70 ℃, the rotating speed of a peristaltic pump is 12+/-3 r/min, the atomization pressure is 0.2+/-0.05 MPa, and the fan power is 50+/-5 Hz.
Further, the preparation method of the bacterial mud comprises the following steps: activating strains to obtain single bacterial colonies; inoculating and culturing single colony to obtain seed solution; transferring the seed liquid into an MRS liquid culture medium for expansion culture to obtain an expansion culture liquid; centrifuging the expanded culture solution to collect bacterial mud, re-suspending the bacterial mud in sterilized 0.85% physiological saline to obtain bacterial suspension, and performing heat shock treatment; and centrifuging again after the heat shock treatment is finished to obtain the final bacterial mud.
Further, the method for activating the strain comprises the following steps: and (3) scribing the strain on an MRS plate, picking a single colony, inoculating the single colony to an MRS liquid culture medium, culturing for 10-14 h at 37 ℃ and carrying out passage for 2-3 times.
Further, the conditions for the expansion culture are 37 to 45℃for 10 to 14 hours, preferably 37℃for 12 hours.
Further, the heat shock treatment conditions are as follows: placing the bacterial suspension in a water bath kettle at 45-60 ℃ for heat shock for 5-20 min; preferably at 55deg.C for 10min.
Further, the centrifugation condition is 3000-6000 rpm/min for 8-20 min; preferably at 5000rpm/min for 10min.
Compared with the prior art, the invention has the following advantages and effects:
The invention provides application of edible fungus polysaccharide in spray drying of Pediococcus acidilactici. The edible fungus polysaccharide can obviously improve the viable count of the pediococcus acidilactici in the spray drying process, and the viable count of the pediococcus acidilactici can reach more than 1.0X10 12 cfu/g by taking Cordyceps militaris polysaccharide, hericium erinaceus polysaccharide, tremella polysaccharide, black fungus polysaccharide and agaricus blazei murill polysaccharide as a spray drying protective agent. Meanwhile, the production process is economical and convenient, continuous production can be realized, the flavor and nutrition of the fungus powder are enriched by adding the edible fungus polysaccharide, and the application potential of the pediococcus acidilactici in the probiotic market is widened.
Detailed Description
The present invention will be described in further detail with reference to examples, but embodiments of the present invention are not limited thereto.
Examples 1 to 5 and comparative examples 1 to 10 below used Pediococcus acidilactici (Pediococcus acidilactici) supplied by the China center for type culture Collection (CICC).
The following comparative examples 11 to 15 employed Lactobacillus fermentum (Lactobacillus fermentum) supplied by the China center for type culture Collection (CICC).
(1) Preparing a culture medium:
MRS Medium (g/L): 10g of beef extract, 10g of peptone, 5g of yeast extract powder, 20g of glucose, 5g of tween-801 g of anhydrous sodium acetate, 2g of dipotassium hydrogen phosphate, 2g of diammonium hydrogen citrate, 0.2g of magnesium sulfate heptahydrate, 0.054g of manganese sulfate monohydrate, and adjusting the pH to 6.5+/-0.1 by constant volume of sterile water. Wherein, the solid culture medium is added with 15-20 g of agar powder on the basis, the liquid culture medium is not added, and the culture medium is sterilized for 15-20 min at 121 ℃.
Beef extract, peptone, yeast extract powder, glucose, anhydrous sodium acetate, dipotassium hydrogen phosphate, diammonium hydrogen citrate, magnesium sulfate heptahydrate, manganese sulfate monohydrate, tween: analytically pure, national drug group chemical reagent limited;
(2) Cordyceps militaris polysaccharide (lot number RLSW 20230823), hericium erinaceus polysaccharide (lot number RLSW 20231211), tremella polysaccharide (lot number RLSW 20231203), auricularia polysaccharide (lot number RLSW 20231117), agaricus blazei polysaccharide (lot number RLSW 20231219) were purchased from Taobao (selenium peptide food raw material company store) of Shanxi Rong Biotechnology Co., ltd, and had a purity of 50%; the xylo-oligosaccharide, fructo-oligosaccharide, isomaltooligosaccharide, maltodextrin, resistant dextrin, beta-cyclodextrin, inulin, sea buckthorn peptide and ovalbumin peptide are all food grade.
The method for detecting the viable count of the pediococcus acidilactici comprises the following steps: the national standard GB4789.35-2016 food safety national standard food microbiology detection of lactobacillus detection is adopted.
Example 1
(1) Strain activation: dipping pediococcus acidilactici preserved in an ultralow temperature refrigerator at the temperature of minus 80 ℃ into a loop by using an inoculating loop, scribing on an MRS solid culture medium, reversely culturing for 24-48 hours at the constant temperature of 37 ℃, picking single bacterial colonies with complete forms, and inoculating the single bacterial colonies into the MRS liquid culture medium for culturing in a constant temperature incubator at the temperature of 37 ℃; after the bacterial liquid is turbid, the bacterial liquid is inoculated into MRS liquid culture medium by a liquid-transfering gun with an inoculum size of 2% (v/v, mL/mL), and the culture is continued for 12 hours at the constant temperature of 37 ℃, and the operation is repeated, and the bacterial liquid is activated for 2 to 3 generations. To ensure the purity of the target strain, the morphology of the microorganism during the propagation was observed with an optical microscope. Taking a certain amount of Cordyceps militaris polysaccharide powder, preparing 10% (w/v, g/mL) of an aqueous solution of Cordyceps militaris polysaccharide, wherein w refers to the mass of the Cordyceps militaris polysaccharide powder, and sterilizing at 85 ℃ for 30min in a sterilizing pot.
(2) Inoculating activated pediococcus acidilactici into MRS liquid culture medium according to an inoculum size of 5%, standing and culturing at 37 ℃ for 12 hours until the pedigree period is reached, centrifuging at 4 ℃ at 5000rpm/min for 10 minutes, collecting bacterial mud, washing with 0.85% physiological saline for 1-2 times, re-suspending in the sterilized 0.85% physiological saline to obtain bacterial suspension, and carrying out hot water bath heat shock treatment at 55 ℃ for 10 minutes. And centrifuging again after the heat shock treatment is finished to obtain the final bacterial mud. Adding sterilized 10% Cordyceps militaris polysaccharide water solution according to the mass ratio of bacterial mud to Cordyceps militaris polysaccharide of 1:5, mixing thoroughly to obtain sample liquid, spray drying at air inlet temperature of 90 deg.C, air outlet temperature of 65+ -3deg.C, peristaltic pump rotation speed of 12r/min, atomization pressure of 0.2MPa, and fan power of 50Hz to obtain Pediococcus acidilactici bacterial powder.
Ten times of the prepared pediococcus acidilactici bacterial powder is serially diluted to a proper multiple, 100 mu L of the pediococcus acidilactici bacterial powder is coated with glass beads, three parallel plates are arranged, and the viable count is calculated after culturing for 24-48 hours at 37 ℃.
Example 2
Compared with example 1, the difference is that according to the bacterial sludge: the mass ratio of the hericium erinaceus polysaccharide to the sterilized 10% (w/v) hericium erinaceus polysaccharide aqueous solution is 1:5, and the rest steps are the same as in example 1, wherein the hericium erinaceus polysaccharide aqueous solution is fully and uniformly mixed to obtain a sample solution.
Example 3
Compared with example 1, the difference is that according to the bacterial sludge: the mass ratio of tremella polysaccharide is 1:5, sterilized 10% (w/v) tremella polysaccharide aqueous solution is added, and the mixture is fully and uniformly mixed to obtain sample liquid, and the rest steps are the same as those of the example 1.
Example 4
Compared with example 1, the difference is that according to the bacterial sludge: adding sterilized 10% (w/v) aqueous solution of Auricularia auricula polysaccharide at a mass ratio of 1:5, and mixing thoroughly to obtain sample solution, wherein the rest steps are the same as those of example 1.
Example 5
Compared with example 1, the difference is that according to the bacterial sludge: the mass ratio of the agaricus blazei polysaccharide to the sterilized 10% (w/v) agaricus blazei polysaccharide aqueous solution was 1:5, and the rest steps were the same as in example 1.
Comparative example 1
Compared with example 1, the difference is that according to the bacterial sludge: the sterilized 10% (w/v) aqueous solution of isomaltooligosaccharide was added at a mass ratio of isomaltooligosaccharide of 1:5, and mixed well to give a sample solution, and the rest steps were the same as in example 1.
Comparative example 2
Compared with example 1, the difference is that according to the bacterial sludge: the mass ratio of the xylo-oligosaccharide is 1:5, the sterilized 10% (w/v) xylo-oligosaccharide aqueous solution is added, and the mixture is fully and uniformly mixed to obtain a sample solution, and the rest steps are the same as those of the example 1.
Comparative example 3
Compared with example 1, the difference is that according to the bacterial sludge: the mass ratio of fructo-oligosaccharide to 5 is 1:10% (w/v) of sterilized fructo-oligosaccharide aqueous solution is added, and the mixture is fully and uniformly mixed to obtain a sample solution, and the rest steps are the same as in example 1.
Comparative example 4
Compared with example 1, the difference is that according to the bacterial sludge: the mass ratio of the resistant dextrin to the sterilized 10% (w/v) resistant dextrin aqueous solution is 1:5, and the mixture is fully and uniformly mixed to obtain a sample solution, and the rest steps are the same as in example 1.
Comparative example 5
Compared with example 1, the difference is that according to the bacterial sludge: the mass ratio of maltodextrin to sterilized 10% (w/v) aqueous solution of maltodextrin was added at 1:5, and the mixture was thoroughly and uniformly mixed to give a sample solution, and the rest of the steps were the same as in example 1.
Comparative example 6
Compared with example 1, the difference is that according to the bacterial sludge: the sterilized 10% (w/v) aqueous solution of beta-cyclodextrin was added at a mass ratio of beta-cyclodextrin of 1:5, and mixed well to give a sample solution, the remaining steps being the same as in example 1.
Comparative example 7
Compared with example 1, the difference is that according to the bacterial sludge: the mass ratio of inulin is 1:5, sterilized 10% (w/v) inulin aqueous solution is added, and the mixture is fully and uniformly mixed to obtain a sample solution, and the rest steps are the same as in example 1.
Comparative example 8
Compared with example 1, the difference is that according to the bacterial sludge: the mass ratio of the sea buckthorn peptide is 1:5, 10% (w/v) sea buckthorn peptide water solution which is sterilized is added, and the mixture is fully and uniformly mixed to obtain a sample solution, and the rest steps are the same as those of the example 1.
Comparative example 9
Compared with example 1, the difference is that according to the bacterial sludge: the mass ratio of the ovalbumin peptide is 1:5, a sterilized 10% (w/v) ovalbumin peptide aqueous solution is added, and the mixture is fully and uniformly mixed to obtain a sample solution, and the rest steps are the same as those of the example 1.
Comparative example 10
Compared with example 1, the difference is that according to the bacterial sludge: the sterilized 0.85wt% physiological saline solution was added at a physiological saline mass ratio of 1:5, and mixed well to obtain a sample solution, and the remaining steps were the same as in example 1.
Comparative example 11
Compared with example 1, the difference is that the bacterial sludge is prepared by (lactobacillus fermentum): the mass ratio of Cordyceps militaris polysaccharide is 1:5, sterilized 10% (w/v) Cordyceps militaris polysaccharide aqueous solution is added, and the mixture is fully and uniformly mixed to obtain a sample solution, and the rest steps are the same as those of the example 1.
Comparative example 12
Compared with example 1, the difference is that the bacterial sludge is prepared by (lactobacillus fermentum): the mass ratio of the hericium erinaceus polysaccharide to the sterilized 10% (w/v) hericium erinaceus polysaccharide aqueous solution is 1:5, and the rest steps are the same as in example 1, wherein the hericium erinaceus polysaccharide aqueous solution is fully and uniformly mixed to obtain a sample solution.
Comparative example 13
Compared with example 1, the difference is that the bacterial sludge is prepared by (lactobacillus fermentum): the mass ratio of tremella polysaccharide is 1:5, sterilized 10% (w/v) tremella polysaccharide aqueous solution is added, and the mixture is fully and uniformly mixed to obtain sample liquid, and the rest steps are the same as those of the example 1.
Comparative example 14
Compared with example 1, the difference is that the bacterial sludge is prepared by (lactobacillus fermentum): adding sterilized 10% (w/v) aqueous solution of Auricularia auricula polysaccharide at a mass ratio of 1:5, and mixing thoroughly to obtain sample solution, wherein the rest steps are the same as those of example 1.
Comparative example 15
Compared with example 1, the difference is that the bacterial sludge is prepared by (lactobacillus fermentum): the mass ratio of the agaricus blazei polysaccharide to the sterilized 10% (w/v) agaricus blazei polysaccharide aqueous solution was 1:5, and the rest steps were the same as in example 1.
TABLE 1 different protectants and viable count after spray drying
As is clear from Table 1, when edible fungus polysaccharide is applied to spray drying of Pediococcus acidilactici, the number of living fungus is higher than that of peptide protectants represented by sea buckthorn peptide and ovalbumin peptide, oligosaccharide protectants represented by isomaltooligosaccharide, xylooligosaccharide, fructooligosaccharide and the like, and dextrin protectants represented by resistant dextrin, maltodextrin, beta-cyclodextrin and the like, the number of living fungus can reach more than 1.0X10 12 cfu/g, and the edible fungus polysaccharide can be used as a novel protectant for preparing high-activity spray drying fungus powder of Pediococcus acidilactici.
The embodiments described above are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the embodiments described above, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principles of the present invention should be made in the equivalent manner, and are included in the scope of the present invention.
Claims (10)
1. The application of edible fungus polysaccharide in preparing pediococcus acidilactici spray drying protective agent is characterized in that: the edible fungus polysaccharide is any one of Cordyceps militaris polysaccharide, hericium erinaceus polysaccharide, tremella polysaccharide, auricularia polysaccharide and Agaricus blazei polysaccharide.
2. The pediococcus acidilactici spray drying protective agent containing edible fungus polysaccharide is characterized in that: the edible fungus polysaccharide is any one of Cordyceps militaris polysaccharide, hericium erinaceus polysaccharide, tremella polysaccharide, auricularia polysaccharide and Agaricus blazei polysaccharide.
3. Use of a spray-drying protectant as claimed in claim 2 in the spray-drying of pediococcus acidilactici.
4. A use according to claim 3, characterized in that:
the application is applied to the improvement of the number of the live bacteria of the Pediococcus acidilactici spray drying.
5. Use according to claim 3 or 4, characterized in that:
the edible fungus polysaccharide is an aqueous solution of edible fungus polysaccharide with the concentration of 10+/-1 percent.
6. The use according to claim 5, characterized in that:
The application is as follows: preparing bacterial suspension, carrying out heat shock treatment on the bacterial suspension, centrifuging to obtain bacterial mud, and mixing the bacterial mud with an edible fungus polysaccharide aqueous solution according to a mass ratio of 1: 3-7, and then spray drying, wherein the parameters of the spray drying are as follows: the air inlet temperature is 90+/-2 ℃, the air outlet temperature is controlled at 60 ℃ -70 ℃, the rotating speed of a peristaltic pump is 12+/-3 r/min, the atomization pressure is 0.2+/-0.05 MPa, and the fan power is 50+/-5 Hz.
7. The use according to claim 6, characterized in that:
The preparation of bacterial suspension, heat shock treatment of the bacterial suspension, and centrifugation to obtain bacterial mud, wherein the bacterial mud is as follows: activating strains to obtain single bacterial colonies; inoculating and culturing single colony to obtain seed solution; transferring the seed liquid into an MRS liquid culture medium for expansion culture to obtain an expansion culture liquid; centrifuging the expanded culture solution to collect bacterial mud, re-suspending the bacterial mud in sterilized 0.85% physiological saline to obtain bacterial suspension, and performing heat shock treatment; and centrifuging again after the heat shock treatment is finished to obtain the final bacterial mud.
8. The use according to claim 7, characterized in that:
the heat shock treatment conditions are as follows: placing the bacterial suspension in a water bath kettle at 45-60 ℃ for heat shock for 5-20 min.
9. The use according to claim 7, characterized in that:
The heat shock treatment conditions are as follows: the bacterial suspension is placed in a water bath kettle with the temperature of 55 ℃ for heat shock for 10min.
10. The use according to claim 7, characterized in that:
the strain is activated as follows: scribing strains on an MRS flat plate, picking single colonies, inoculating the single colonies to an MRS liquid culture medium, culturing for 10-14 h at 37 ℃ and carrying out passage for 2-3 times;
the conditions of the expansion culture are 37-45 ℃ for 10-14 h;
The centrifugal condition is 3000-6000 rpm/min for 8-20 min.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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