CN109650553A - It is harnessed a river the method and its application of pollution using facultative heterotrophic microorganism - Google Patents

It is harnessed a river the method and its application of pollution using facultative heterotrophic microorganism Download PDF

Info

Publication number
CN109650553A
CN109650553A CN201811564826.5A CN201811564826A CN109650553A CN 109650553 A CN109650553 A CN 109650553A CN 201811564826 A CN201811564826 A CN 201811564826A CN 109650553 A CN109650553 A CN 109650553A
Authority
CN
China
Prior art keywords
river
pollution
water
harnessed
heterotrophic microorganism
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201811564826.5A
Other languages
Chinese (zh)
Inventor
周刚
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN201811564826.5A priority Critical patent/CN109650553A/en
Publication of CN109650553A publication Critical patent/CN109650553A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/30—Aerobic and anaerobic processes
    • C02F3/301—Aerobic and anaerobic treatment in the same reactor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/001—Runoff or storm water
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/06—Contaminated groundwater or leachate
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/34—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

It the present invention relates to the use of facultative heterotrophic microorganism to harness a river the method for pollution, include the following steps: that launching original Rhodopseudomonas palustris in target water carries out adaptability bacteria selection and optimization, prepared by the batch of preparation needed for then being carried out with the bacterial strain filtered out；The Rhodopseudomonas palustris preparation for launching above-mentioned batch preparation in proportion to target water is administered.Of the invention to be harnessed a river the method for pollution using facultative heterotrophic microorganism, wherein the bacterium is low to the environmental requirement of application, and adaptive capacity is very strong, in oligotrophic and eutrophic water body can normal growth, waters, river suitable for various territorial environments.Directly the polluter in water body can be consumed and be utilized, resource utilization will be polluted.After organic pollutant is reduced, a large amount of organic matter decomposes required oxygen under saving, and river water starts reoxygenation, gradually restores to promote water quality rapidly on the basis of solving black and odorous water, provides life condition for aquatic animal.

Description

It is harnessed a river the method and its application of pollution using facultative heterotrophic microorganism
Technical field
It the present invention relates to the use of facultative heterotrophic microorganism to harness a river the method and its application of pollution.
Background technique
In recent years, due to social economy's high speed development, China is faced with water environment, water resource, Ecology and water disaster etc. The most pressing water problems of four classes.Their outstanding behaviours are composite water pollution, and its transfer in basin, lake is comprehensive Shortage of water resources and drinking water safety problem, the ecological environment destruction that water conservancy, hydroelectric project cause, and due to bloods and droughts and Disaster that contamination accident etc. constitutes comprehensive basin, lake is paddled.At this stage, basin-wide water pollution is becoming restriction China The bottleneck of development needs that aggregate measures is taken to solve.
It is such as a large amount of to use high phosphorous detergent and the change rich in phosphorus, nitrogen due to inappropriate, the unreasonable activity of the mankind Untreated people, fowl, animal manure and sanitary sewage, industrial wastewater etc. are discharged into open waters wantonly, greatly exceed river by fertilizer The ability of the natural self-regeneration in river and lake sea, self purification, it is necessary to take the mode of manual intervention to remove pollution sources, restore its original The benign water ecological environment having.
Summary of the invention
It is harnessed a river the method for pollution an object of the present invention is to provide a kind of using facultative heterotrophic microorganism.
It is of the invention to be harnessed a river the method for pollution using facultative heterotrophic microorganism, include the following steps: S101: in target Waters launches original Rhodopseudomonas palustris and carries out adaptability bacteria selection and optimization, then carries out institute with the bacterial strain filtered out The batch of preparation is needed to prepare；
S102: the Rhodopseudomonas palustris preparation for launching above-mentioned batch preparation in proportion to target water is administered.
It is of the invention to be harnessed a river the method for pollution using facultative heterotrophic microorganism, wherein environmental requirement of the bacterium to application Low, adaptive capacity is very strong, the equal energy normal growth in oligotrophic and eutrophic water body, suitable for various territorial environments Waters, river.It is administered in the waters river of pollution using the method for present specification, utilizes the red vacation in the marsh of facultative heterotrophism Monad can be under conditions of not oxygen consumption, the characteristics of breeding using organic matter as nutrition, directly by the pollutant in water body Matter consumption utilizes, and by the food chain of bacterium, flea, fish, will pollute resource utilization.It is a large amount of under saving after organic pollutant is reduced Organic matter decomposes required oxygen, and river water starts reoxygenation, gradually restores to promote water quality rapidly on the basis of solving black and odorous water, is Aquatic animal provides life condition.Waters aquatic ecosystem can be established and restored in a short time.
The Technical investment is small, quick, is not required to engineering measure, can also realize considerable economic effect by " dirt~fish converts " Benefit.Pollution is generated by resource utilization, irredundant substance.Strain source is clear, the mechanism that carries weight verifying, inanimate object safety it Anxiety.
It is harnessed a river the method for pollution, can also be had as follows using facultative heterotrophic microorganism in addition, the present invention is above-mentioned Additional technical characteristic:
Further, the condition of the bacterial strain is prepared in the step S101 are as follows: temperature is 28 DEG C~32 DEG C facultative Illumination condition；PH value is 7.0；And make buffer using potassium dihydrogen phosphate and dipotassium hydrogen phosphate；With ammonium chloride, magnesium sulfate, sulfuric acid Ferrous iron, EDTA, beef extract, B family vitamin, microelement are nutrition.
Further, the concentration of the Rhodopseudomonas palustris bacterial strain described in the step S102 is 3ppm~5ppm.
It further, further include following steps in the step S102: when the primary fish lazy weight in river, by mesh Every cubic metre of waters volume of mark supplies filter-feeding fish 45g~55g.
Further, in the step S102, continue to put into the marsh when the water quality monitoring index is not up to standard The red unicellular bacteria strain of vacation, launching the period is 5 days~6 days.
It is another object of the present invention to propose that the method is organic in municipal sewage, wastewater from chemical industry, high concentration River caused by waste water, feces of livestock and poultry and domestic rubbish disposal it is black smelly and promoted water quality in terms of application.
Additional aspect and advantage of the invention will be set forth in part in the description, and will partially become from the following description Obviously, or practice through the invention is recognized.
Detailed description of the invention
Fig. 1 is the schematic diagram of the PHB particle in RhP microorganism fungus kind rich in linear polyphosphoric acid polymer；
Fig. 2 is the schematic diagram that RhP system technology participates in waters substance circulation.
Specific embodiment
The embodiment of the present invention is described below in detail, examples of the embodiments are shown in the accompanying drawings, wherein from beginning to end Same or similar label indicates same or similar element or element with the same or similar functions.Below with reference to attached The embodiment of figure description is exemplary, it is intended to is used to explain the present invention, and is not considered as limiting the invention.
The earth is one and recycles ability life and growth in nature ecosystem by substance, and sunlight, water, nutrition, life constitute week And the substance renewed moves in circles, so-called pollution may be exactly only to seek to other life forms (such as microorganism) for people It supports, so there is no real wastes.
The technological core of present specification be by polluted water region dispensing can be with various organic and inorganic dirt in water body Dye source is the Rhodopseudomonas palustris strain that nutrition carries out flourish, and own is used as the bait of zooplankter to be consumed again, Zooplankter is used as the food of the large-scale aquatic animal class such as fish, shellfish, shrimp again, and terricole passes through predaceous fish, shellfish, shrimps, most Pollutant is consumed and is taken from water body at last.On the basis of reconstructing aquatic food chain, accelerates the substance circulation of polluted water region, strengthen The self-purification capacity of water body, cuts off the nutrition supply of harmful algae, administers water pollution to reach, prevents algal bloom and pollution Physical resourcesization utilize purpose.
The Rhodopseudomonas palustris of present specification selection is the prokaryotes occurred earliest on the earth, it is photosynthetic with other Biology together forms the original producer in the nature ecosystem, plays an important role in the substance conversion of nature, Energy conversion and the banking system of economical rationality are provided for earth ecology circle.Rhodopseudomonas palustris strain is by China Microbiological Culture presevation committee common micro-organisms center provides, and by spontaneons screening, is isolated and purified obtained with relatively strong heredity The indigenous strain (strain) of stability, is identified by Institute of Micro-biology, the Chinese Academy of Sciences, has been carried out in China General Microbiological collection special Easy storage.The bacterium has ingester in all waters, can be consumed by food chain.Its reproductive order of generation in water body has Limit, withers away after general 10 days, and controllability is very strong.
Strain is originated from China's natural ecosystems, it is widely distributed in ocean, waters, rivers, sludge, in soil, passes through Researcher is repeatedly enriched with and separates, and gradually forms the bacterial strain for being referred to as " RhP Multifunctional water improves bacterium " for stablizing heredity.It has both The function of chemical heterotrophism, autotrophy and photoheterotrophy, autotrophy is the main force of cycle of matter in the earth.
The study found that Rhodopseudomonas palustris has assimilation NH+ 4Effect, can by specific reaction synthesize body needed for A variety of amino acid, to reduce the content of nomadic nitrogen in water body.
Rhodopseudomonas palustris needs to consume in reproductive process a large amount of phosphorus as the structural material of cell, so its is right There is very important the reduction of phosphorus in water body.
Rhodopseudomonas palustris H2S makees hydrogen donor, can be reduced into element S and store in the cell or be deposited on cell Outside, toxic action is reduced.
The bacterium can generate in the environment rich in phosphorus to be accounted for the PHB of cell volume 40%-50% and includes (cytoplasmic Inclusions), being the storage of linear polyphosphoric acid polymer by the Ruminants animal that can not be consumed from intake in environment and temporarily Come.When water body phosphate is lower than threshold value, the linear polyphosphoric acid polymer in PHB is decomposed into phosphate again, and it is thin to become synthesis The important source material of the cellular components such as after birth, protein, DNA.Therefore, the bacterium is fast to the degradation speed of phosphorus in water body, effect is good.
Equally, ammonia nitrogen excessive in water body also can pass through amino during subsequent growth and breeding with similar mechanism Sour route of synthesis participates in the synthesis of cell protein component.
Molecular mechanism research shows that Rhodopseudomonas palustris can pass through non-beta-oxidation and benzoyl coacetylase virtue using luminous energy Fragrant ring reduction approach degradation benzene pesticide residue；The bacterium is higher than common bacteria to the tolerance of the heavy metal ion such as mercury, lead, arsenic, copper by 1 To 2 orders of magnitude.Under the concentration of heavy metal ion of specificity domestication, the adsorption capacity of bacterium may be up to 85% to 98%.
Rhodopseudomonas palustris can not only be under conditions of anaerobism illumination with lower fatty acid, a variety of dicarboxylic acids, alcohols, sugar The organic matters such as class, aromatic compound and carbon dioxide etc. carry out photoheterotrophy growth as electron donor, and can be micro- good Under oxygen dark condition, using above-mentioned organic matter as respiratory substrate, aerobic heterotrophic growth is carried out.Therefore, it is both not as aerobic activity Sludge bacteria is got dirty the limitation of oxygen in water like that, be can use luminous energy and is carried out efficient energetic supersession, even faint light Also it can utilize；Again unlike the methane bacteria of strictly anaerobic is to sensitive oxygen, it can under conditions of aerobic decomposing organic matter, Energy is obtained by oxidative phosphorylation.
Period, certainly also can some nitrogenous, phosphorus inorganic salts utilized by algae, along the original food chain of water body into Row substance circulation, but because great changes have had occurred in its concentration and structure, the value volume and range of product of generated algae will not be blue The pattern that green alga single cell species dominate, the bio-diversity in water body is to emerge from.This process is practical just It is that the substance circulation scene of uncontaminated water body low speed is passed through into artificial bioid intervention in the case where water eutrophication Afterwards, the approach consumed because increasing a pollution, makes water body that the substance recurrent state of high speed be presented, and also just reaches fast degradation dirt The purpose of dye.
The technological core of present specification is to utilize organic or inorganic in Rhodopseudomonas palustris growth consumption polluted water region Nitrogen phosphate and sulfur, carbon of state etc. are that nutrition is proliferated, and by direct saprophytic conversion, control water pollution, cutting pollution tolerance Extremely strong, fish are difficult to the nutrition supply of the cyanobacteria digested.It is clear that can thus establish a quick food chain, accelerate The substance of polluted water region recycles, and promotes the self-purification capacity of water body.
Rhodopseudomonas palustris is multi-party in nutrient competition, adaptability of existence, growth high efficiency and strain stability etc. Face obtains important breakthrough, currently has been applied to administer the microorganism formulation of large-scale water pollution to become.
Microorganism is that most important analyst and potentiality to be exploited are maximum in terrestrial ecosystem, the most valuable money of the mankind Source library.It can be played in the regeneration of the Degradation and Transformation, resource of pollutant, the in terms of microorganism of ecological environment Important function.Environmental pollution that mankind nowadays are faced, shortage of resources, ecological disruption, health are aggrieved etc. many great to ask Topic, is likely to search out solution from the developmental research of microbial resources.Just because of this, developed country was from 80 years A large amount of human and material resources have been put into the exploitation of environmental microorganism, Applied research fields since generation, have achieved many achievements, together When rapidly developed in terms of the commercialization of microbial technique, industrialization, achieve huge economy, environmental and social benefits.
With China's environmental management dynamics continue to increase and horizontal raising, in order to reach sewage purification, Treatment of Sludge, Black smelly equal improvement purpose is disposed and eliminated to Domestic Waste Minimization, while avoiding and reducing secondary pollution again, at microorganism The demand of reason technology is just becoming increasing.In recent years, China occurs much from the micro- of the foreign countries such as Japan, France and the U.S. Biological product.
Domestic many colleges and universities and research institution put into man power and material, and research and development is used for efficient micro- life of pollution control Produce product, and in terms of municipal sewage, wastewater from chemical industry, high concentrated organic wastewater, feces of livestock and poultry and domestic rubbish disposal disposition With application.
The scope of application:
1, for being chronically at industrial pollution, Rural Plane Source Pollution, the black smelly water in river caused by town dweller's domestic pollution Domain is quickly repaired and is improved, and ecology is gradually restored.
Typical case: the year regular harnessing project of 19 kilometers of Anyue Sichuan Yueyang pollution of estuary.
2, the improvement and maintenance in large-scale landscape waters, ecological wetland.
Typical case: Beijing Green Lake National Urban Wetland Park water ecological restoration engineering.
3, it substitutes small towns sewage treatment plant and the life in small towns, production waste is focused on by a constant volume with very low cost Lake and reservoir, after the advanced treating by RhP system technology, water outlet reaches earth's surface water quality standard, can be subsidized with aquaculture profit Part processing cost realizes pollution recycling.
Typical case: the Sichuan Wusheng County town Wan Shanchang domestic sewage treatment engineering.
4, breeding waste innoxious use realizes circular economy.
Typical case: entering crawl after the gold wheel swine waste marsh gas of Guanghan, breed fish after RhP process, water body reuse
The biological safety of Rhodopseudomonas palustris illustrates:
" Rhodopseudomonas palustris " strain is provided by China Microbial Culture Preservation Commission's common micro-organisms center, is passed through Spontaneons screening is isolated and purified the indigenous strain (strain) obtained with stronger genetic stability, passes through Chinese Academy of Sciences microorganism It is identified, carries out Patent Deposit in China General Microbiological collection.
" Rhodopseudomonas palustris " of present specification first is strain in Ministry of Agriculture's available directories, by close scrutiny, Obtaining production licence, (feeding, which adds (2003) 1556 approvals and executes Q/63316440-0.2-2011 standard, to be produced, and is widely answered In aquaculture.
Secondly the bacterium has ingester in all waters, can be consumed by food chain.Moreover it is numerous in water body It is limited to grow algebra, withers away after general 10 days, controllability is very strong.
The biological safety of this technology is in multiple engineering projects of our unit's execution and large-scale more than ten years of waters simultaneously Application in sufficiently verified.
Furthermore this product passes through the zebra fish acute toxicity test of Chinese Center for Disease Control, large cyclop acute toxicity reality Test, small white mouse Acute oral tox-hty test, algal grown inhibit test be confirmed that it is nontoxic level microbe, have good biology Safety.
1, implementation steps
It is of the invention to be harnessed a river the method for pollution using facultative heterotrophic microorganism, include the following steps:
(1) Rhodopseudomonas palustris is launched in target water first and carry out adaptability bacteria selection and optimization, then use The bacterial strain filtered out is in condition are as follows: the facultative illumination condition that temperature is 28 DEG C~32 DEG C；PH value is 7.0；And utilize biphosphate Potassium and dipotassium hydrogen phosphate make buffer；With ammonium chloride, magnesium sulfate, ferrous sulfate, ethylenediamine tetra-acetic acid, beef extract, the dimension life of B race Element and microelement are the Rhodopseudomonas palustris preparation described in preparing under conditions of nutrient.
(2) the Rhodopseudomonas palustris bacterial strain is launched in target water to be administered.Advantageously, described in periodic monitoring The COD of sewageMN, transparency, TP, NH3- N, DO, pH value, water temperature and temperature parameter simultaneously continue to put into when the parameter is not up to standard The Rhodopseudomonas palustris bacterial strain.Advantageously, concentration of the Rhodopseudomonas palustris bacterial strain in sewage be 3ppm~ 5ppm, each dispensing period are 5 days~6 days.
(3) when the primary fish lazy weight in river, according to target every cubic metre of waters volume supply filter-feeding fish 45g~ 55g。
Of the invention to be harnessed a river the method for pollution using facultative heterotrophic microorganism, wherein Rhodopseudomonas palustris is to application Environmental requirement it is low, adaptive capacity is very strong, in oligotrophic and eutrophic water body can normal growth, be suitable for it is various Waters, the river of territorial environment.It is administered in the waters river of pollution using the method for present specification, to being dissolved in water body Oxygen has biggish promotion, will play facilitation for the growth of the stock of fish, can establish in a short time and to restore waters aquatic The ecosystem.Investment can embody for sensorial effects one month less than the 50% of traditional technology, by " dirt~fish converts ", realize Considerable economic benefit.Pollution is generated by resource utilization, irredundant substance.Strain source to China natural water, through authoritative department Verifying, the anxiety of inanimate object safety.
2, management goal
After the status to different waters is analyzed, following phased goal is determined to project:
(1) after administering 1 month, waters black and odorous water phenomenon is eliminated, sensory effects have apparent improvement, and water transparency has Larger promotion, water quality indicator make moderate progress；
(2) after administering 3 months, decline 50% before the main water quality indicator in waters is relatively administered；
(3) after administering 180 days, main water quality monitoring index presses 2002 standard upgrading of GB3838-, two ranks.
3, monitoring parameters and scheme
According to the pollution situation and existence conditions in waters, generally selection CODMN, transparency, TP, NH3- N, DO, pH value, water The parameters such as temperature, temperature are as monitoring objective.The bed mud sample of fixed point acquisition at times simultaneously, investigates different time sections indsole mud sample sheet In above-mentioned each parameter situation of change, converse the reduction of bed mud thickness.
Qualified third party monitoring mechanism is selected to carry out spot sampling monitoring.
Four, technical guarantee and it is expected that achievement
The Institute for Applied Technology great Kai, Chengdu that inventor is set up is to specialize in microbial technique research, production, application Scientific and technical innovation enterprise, the RhP system technology with independent intellectual property rights and RhP Multifunctional water of research and development improve bacterium, As the core technology product that waters, reservoir pollution prevent and treat, the approval production of Ministry of Agriculture's production licence is obtained.Water is being carried out for a long time During prevention and cure of pollution, as the offer unit of technology and product, intend or assume responsibility for several water pollution control projects certainly.It is early It is proposed is administered in 1997 to the imagination of water pollution with biologic technology, and is dedicated to this in the 20 later years The research and test of aspect, have grasped a large amount of firsthand information, and expert's technical appraisement of multiple administration project gives sufficiently Affirmative.
By school-run enterprise in many years project cooperation, power-power cooperation, it is believed that we are ability to provide sufficient skill for this project Art guarantee.
This technology administers the South Lake water pollution and Blue-green Algae Fast Growth of 11.83 sq-km of Hunan Yueyang, takes Very good effect is obtained, by the bad V class water quality of earth's surface, administers to ground Table IV class water quality, meets the functional requirement in the waters and successfully press down The situation of the lake Blue-green Algae Fast Growth is made.
Five, performance analysis
1, economic benefit
The technical spirit of this project is polluter resource utilization, and certain fishery will be generated by, which administering, takes in.
2, social benefit
Since polluted water region water ecological environment is unsound, water pollution is more serious, seriously affects the function in waters.This Project implementation is intended to while converting various organic contaminations in water body, restore water body by manually adding biological agent Self-purification capacity gradually sets up sound food chain, and the use function and landscape effect also the people one cleaning for restoring water body are relaxed Suitable living space.
3, environmental benefit
The microorganism formulation as used in project can decompose organic compound and benzene, Asia in a variety of sewage and bed mud The substances such as nitric acid, hydrogen sulfide inhibit the breeding of harmful bacteria and virus, eliminate waters and black smelly phenomenon.This project is promoted After, directly effective ecological technique method can be provided for periphery water pollution control and Water Ecological Recovery, will brought obvious Environmental benefit, provide good living environment for waters, also will for social construction create a preferable water environment.
Case introduction
One, Blue-green Algae Fast Growth project is administered in Yueyang South Lake water pollution
The water pollution control of overlarge area and Blue-green Algae Fast Growth engineering is prevented, there are no successful stories in China, also do not have Fixed mode and method can be followed, and Yueyang municipal people's government leader dares to be the first, public using 11.83 side of RhP system technology governance In open waters water pollution, prevent Blue-green Algae Fast Growth engineering.
In July, 2008, the South Lake water area water-quality monitoring data that Yueyang ring prison station provides are shown:
TP average value: 0.563mg/L, TN average value: 4.57mg/L, chlorophyll average value: 142.01mg/ cubic metres, high Manganate average value: 8.22mg/L,
We from July 23rd, 2008 to September 1st, using the nearly improvement in 40 days of RhP system technology, Yueyang environment prison The monitoring data that survey station provides are shown:
TN drops to 2.10mg/L by 4.88mg/L；TP drops to 0.137mg/L by 0.334mg/L；
CODmn6.27mg/L is dropped to by 8.96mg/L；CHIa drops to 48.27mg/L by 99.07mg/L；
Dissolved oxygen rises to 7.88mg/L by 6.96mg/L；Transparency rises to 0.52 meter by 0.25 meter；
Regulation effect:
From August 23 days to 2008 July in 2008 completion the first stage project on the 31st.Significant effect: contain cyanobacteria completely Outburst situation eliminates stink, improves water body organoleptic indicator.
Conclusion: (Yueyang government organization expert group review comment)
What South Lake was administered facts have proved that " intensified ecological technology " is theoretical feasible；
The technical costs is low, quick, meets China's actual conditions；
RhP Multifunctional water improvement bacterium activity is strong, wide adaptability, biological safety are good；
It is world-famous puzzle that the pollution control in open big waters, which inhibits blue algae bloom, and the successful implementation of the engineering will be China's lake pollution, which is administered, provides example, has very high society, economy, environmental benefit.
Two, county, Anyue Sichuan Yueyang river project brief
1, Yueyang river overview
The long river levee section in Yueyang river is located at Anyue County north of the city suburb, and river surface is about 50 meters wide, is about 19 kilometers, and 3 meters of mean depth, Storage capacity is up to 189 ten thousand steres.In Anyue county town downstream.There are about 50,000 tons of Anyues city sanitary sewage and Anyue industry parks daily Area's industrial wastewater and littoral resident living, production waste enter the river, water body serious eutrophication, and self-purification capacity disappears, river Water present ink black and the alternate water colour of milky, whole river gives out strong pungent foul smell, directly contributes along the river The common people cannot use the river water to produce, live.
2, Yueyang river regulation effect
After dead fish event occurs for the long river levee section in the November in 2011 of Anyue County on the 11st Yueyang river, cause Anyue county Party committee, county government Great attention.Aiming at the problem that section water body serious eutrophication, Anyue county Party committee, county government's research have been implemented correlation and have been arranged It applies, connection and communication through related functional department, " the RhP system technology " developed using me is determined, from November 23 in 2011 Day starts to carry out it trimestral Emergency management by a definite date and later period sustainable harnessing, under conditions of a variety of unfavorable factors, emergency Control obtains remarkable effect.
On June 5th, 2013, Bureau of Water Resources, Anyue County hold the long river levee section water eutrophication Emergency management item in Yueyang river Mesh checks and accepts meeting.The heads of the unit such as Bureau of Water Resources, county, aquatic products fish-farming operation office, the Bureau of Finance, Sichuan University and part panel member participate in Meeting.
In meeting, undertaken project unit has carried out thematic report with regard to the long river levee section water eutrophication Emergency management in Yueyang river, Aquatic products fish-farming operation office, county implements field surveillance person and is carried out supervision report, and panel member examines its data, looks into It has seen improvement scene, and has carried out discussion, formd following examination opinion:
The project that the Institute for Applied Technology great Kai, Chengdu undertakes obtains following improvement achievement:
(1), it has stopped Yueyang river and has polluted the impetus further deteriorated；
(2), dead fish phenomenon does not occur for target water, and dissolved oxygen reaches 6mg/L or more in water body；
(3), middle and lower reaches river stink completely eliminates, and upstream pollution sources stink is substantially reduced；
(4), river water body whitens, nigrescence state has been eliminated；
(5), the transparency of entire water body significantly improves, and water colour has larger improvement；
(6), the zooplankter in water body have a large capacity and a wide range, wild fish growth it is vigorous.
Participant unanimously thinks that Emergency management effect is obvious, reaches under conditions of pollution is heavy, temperature is low, is pressed for time Protocol requirement agrees to acceptance(check).
Three, Hefei milky way park water system project brief
1, Hefei milky way park water system overview
Between Huizhou City main road and Jinzhai County road, belt South Road is faced in north in Hefei milky way park, and Wu Hulu is adjoined in south (see Fig. 2). It is in the east well-known Bao Zheng garden scenic spot, the building Xi Liandaoxiang scenic spot.171246 square metres of land occupation.Milky way water system is located at the milky way In park, water surface area is about 50000 square metres, and 2 meters of mean depth.
2, water quality condition before the Governing water system of Hefei milky way park
Water quality monitoring index in milky way park is the bad V class of surface water, as water body continues eutrophication, the natural ecology in waters System, bio-diversity and benign food chain are destroyed, and the very strong cyanobacteria of resistant power grows wild, and cyanobacteria is because of surface quilt Wrap up gelatinous polysaccharose substance to be difficult to by fish, food chain cannot be formed, then for years blue algae bloom at evil, water For body with different smell, water quality is in serious eutrophication situation.
To carry out this improvement, milky way park water system detects on July 2nd, 2015 in Baohe District Environmental Protection Agency.Monitoring Report display (see the table below one), water body are in serious eutrophication, belong to the bad V class water quality of surface water.Especially No. 3 points, chlorophyll a Numerical value be up to 1.29 × 103,；And point TP reaches 7.13mg/L.
1 milky way park of table water monitoring data unit on July 2nd, 2015: mg/L
CODMn NH3-N TN TP Chlorophyll a
No. 1 point 7.04 0.466 3.59 0.533 21.5
No. 2 points 8.27 0.071 3.72 0.465 19.9
No. 3 points 16.7 0.222 3.48 7.13 1.29×103
3, Hefei milky way park Governing water system progress brief introduction
Hefei milky way park Governing water system project started on July 2nd, 2015, until on September 10th, 2015, add up to administer Time has been carried out 70 days or so.
(1), full waters, which is splashed, launches the dispensing of RhP preparation: 11 times；It is accumulative to have launched 11 tons of RhP preparation；Use implementation ship Only 11 times；Implement 33 person-times of employee；RhP preparation long-distance transport: 11 times.
(2), improvement is launched with 2500 kilograms of filter-feeding fish.
4, Hefei milky way park Governing water system effect
Baohe District Environmental Protection Agency is respectively at August in 2015 7 days, on August 12nd, 2015 to milky way public affairs after administering a wheat harvesting period Garden Governing water system effect carries out tracing detection.Monitoring report shows and (see the table below 2, table 3), and every water quality indicator declines to a great extent, And sensorial effects have substantially improved, water surface cyanobacterial bloom largely reduces, and water transparency increases, and water body different smell is It eliminates.
2 milky way park of the table water monitoring data unit on the 7th of August in 2015: mg/L
CODMn NH3-N TN TP Chlorophyll a
No. 1 point 2.0 0.398 1.79 0.412 9.06
No. 2 points 2.0 0.312 1.73 0.305 8.65
No. 3 points 2.9 0.309 1.68 0.269 8.1
3 milky way park of the table water monitoring data unit on the 12nd of August in 2015: mg/L
CODMn NH3-N TN TP Chlorophyll a
No. 1 point < 2.0 0.699 2.14 0.385 9.21
No. 2 points < 2.0 0.792 1.85 0.318 12.1
No. 3 points < 2.0 1.38 2.32 0.493 14.2
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show The description of example " or " some examples " etc. means specific features, structure, material or spy described in conjunction with this embodiment or example Point is included at least one embodiment or example of the invention.In the present specification, schematic expression of the above terms are not It must be directed to identical embodiment or example.Moreover, particular features, structures, materials, or characteristics described can be in office It can be combined in any suitable manner in one or more embodiment or examples.In addition, without conflicting with each other, the skill of this field Art personnel can tie the feature of different embodiments or examples described in this specification and different embodiments or examples It closes and combines.
Although the embodiments of the present invention has been shown and described above, it is to be understood that above-described embodiment is example Property, it is not considered as limiting the invention, those skilled in the art within the scope of the invention can be to above-mentioned Embodiment is changed, modifies, replacement and variant.

Claims (7)

1. a kind of harnessed a river the method for pollution using facultative heterotrophic microorganism, which comprises the steps of:
S101: the screening and optimization that original Rhodopseudomonas palustris carries out Adaptive strain are launched in target water, then with sieve The bacterial strain selected carries out batch preparation；
S102: Rhodopseudomonas palustris preparation prepared by the batch periodically is launched to target water by preset concentration, with right The river of pollution is administered.
2. according to claim 1 harnessed a river the method for pollution using facultative heterotrophic microorganism, which is characterized in that in institute It states in step S101, prepares the bacterial strain in batches in illumination and under conditions of temperature is 28 DEG C~32 DEG C.
3. according to claim 1 harnessed a river the method for pollution using facultative heterotrophic microorganism, which is characterized in that in institute It states in step S101, pH value when preparing the bacterial strain in batches is 7.0.
4. according to claim 1 harnessed a river the method for pollution using facultative heterotrophic microorganism, which is characterized in that in institute It states in step S101, makees buffer using potassium dihydrogen phosphate and dipotassium hydrogen phosphate when preparing the bacterial strain in batches, with ammonium chloride, sulphur Sour magnesium, ferrous sulfate, ethylenediamine tetra-acetic acid, beef extract, B family vitamin and microelement are nutrient.
5. according to claim 1 harnessed a river the method for pollution using facultative heterotrophic microorganism, which is characterized in that in institute It states in step S102, the preset concentration is 3ppm~5ppm, and each dispensing period is 5 days~6 days.
6. according to claim 1 harnessed a river the method for pollution using facultative heterotrophic microorganism, which is characterized in that in institute Stating in step S102 further includes following steps: when river fish lazy weight, according to target supplying filter for every cubic metre of waters volume Feeding fish is to 45g~55g.
7. the described in any item methods of claim 1~6 are in municipal sewage, wastewater from chemical industry, high concentrated organic wastewater, feces of livestock and poultry With the application in terms of the black smelly water quality with promotion in river caused by domestic rubbish disposal.
CN201811564826.5A 2018-12-20 2018-12-20 It is harnessed a river the method and its application of pollution using facultative heterotrophic microorganism Pending CN109650553A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811564826.5A CN109650553A (en) 2018-12-20 2018-12-20 It is harnessed a river the method and its application of pollution using facultative heterotrophic microorganism

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201811564826.5A CN109650553A (en) 2018-12-20 2018-12-20 It is harnessed a river the method and its application of pollution using facultative heterotrophic microorganism

Publications (1)

Publication Number Publication Date
CN109650553A true CN109650553A (en) 2019-04-19

Family

ID=66115955

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201811564826.5A Pending CN109650553A (en) 2018-12-20 2018-12-20 It is harnessed a river the method and its application of pollution using facultative heterotrophic microorganism

Country Status (1)

Country Link
CN (1) CN109650553A (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101591068A (en) * 2009-05-21 2009-12-02 东莞圣源环保科技有限公司 The treatment process of polluted river
KR20110005460A (en) * 2009-07-10 2011-01-18 주식회사 한국한신테크노 Industrial waste, waste water sludge, garbage disposal machine
CN101955291A (en) * 2009-07-14 2011-01-26 周刚 Biomanipulation theory based lake pollution treatment technology
CN102050523A (en) * 2010-12-29 2011-05-11 赵亚勋 Method for treatment and in-situ ecological restoration of black and stink riverway by using microorganism
US20160326035A1 (en) * 2013-09-09 2016-11-10 Reinhard Heuser Method for decontaminating contaminated water and plastics container therefor
CN108179100A (en) * 2018-03-26 2018-06-19 尹康康 A kind of photosynthetic bacteria screening and culturing method

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101591068A (en) * 2009-05-21 2009-12-02 东莞圣源环保科技有限公司 The treatment process of polluted river
KR20110005460A (en) * 2009-07-10 2011-01-18 주식회사 한국한신테크노 Industrial waste, waste water sludge, garbage disposal machine
CN101955291A (en) * 2009-07-14 2011-01-26 周刚 Biomanipulation theory based lake pollution treatment technology
CN102050523A (en) * 2010-12-29 2011-05-11 赵亚勋 Method for treatment and in-situ ecological restoration of black and stink riverway by using microorganism
US20160326035A1 (en) * 2013-09-09 2016-11-10 Reinhard Heuser Method for decontaminating contaminated water and plastics container therefor
CN108179100A (en) * 2018-03-26 2018-06-19 尹康康 A kind of photosynthetic bacteria screening and culturing method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
杨逢乐等: "《高原湖泊低污染水治理技术及应用》", 31 August 2014, 冶金工业出版社 *

Similar Documents

Publication Publication Date Title
Marchaim Biogas processes for sustainable development
CN100378272C (en) Stereo in-situ ecological urban water system repairing process
CN102010112B (en) Method for treating livestock wastes
Hernández et al. Studies on the biofiltration capacity of Gracilariopsis longissima: from microscale to macroscale
CN103951152B (en) Compound formulation of a kind of sustainable reduction river bottom mud and preparation method thereof
CN105174471A (en) Reinforced black odorous river microecological reconstruction/balance and water quality improvement method
CN105967340A (en) Ecological restoration method for eutrophic river channel water body
CN106830523A (en) A kind of method that black-odor riverway comprehensive water-body is administered
CN108640453A (en) A kind of simultaneous water quality reaching standard process of poultry waste nutrient recycling
CN105274029B (en) A kind of Nitrobacter winogradskyi and nitrobacteria-denitrifying bacteria composite bacteria agent and production method and application
CN109879445A (en) A kind of river water quality original position method for improving and system
CN109052654A (en) A method of for improving the smelly water of urban black
CN107673558B (en) Black and odorous water body purification method
CN105016579A (en) Rural sewage treatment system
Lu et al. A review of ammonia-oxidizing archaea and anaerobic ammonia-oxidizing bacteria in the aquaculture pond environment in China
CN109052834A (en) A kind of administering method of eutrophication water
CN101701197B (en) Novel microorganism flora mixture and mixed nutrient medium thereof
Mahesh et al. Advances in biotechnological tools for bioremediation of wastewater using bacterial–algal symbiotic system
Guterstam Demonstrating ecological engineering for wastewater treatment in a Nordic climate using aquaculture principles in a greenhouse mesocosm
Wang The development of ecological wastewater treatment and utilization systems (EWTUS) in China
CN111847778A (en) Method for treating and maintaining landscape water body and pool
Prakash et al. Integrated application of macrophytes and zooplankton for wastewater treatment
CN104591306A (en) Novel formula method and apparatus for removal of riverway cyanobacteria in period of dormancy
CN110577335A (en) Method for promoting food chain operation and accelerating water environment treatment
Wang Ecological waste treatment and utilization systems on low-cost, energy-saving/generating and resources recoverable technology for water pollution control in China

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination