CN1096455C - Process for extracting glycitin and soybean saponin - Google Patents
Process for extracting glycitin and soybean saponin Download PDFInfo
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- CN1096455C CN1096455C CN00105542A CN00105542A CN1096455C CN 1096455 C CN1096455 C CN 1096455C CN 00105542 A CN00105542 A CN 00105542A CN 00105542 A CN00105542 A CN 00105542A CN 1096455 C CN1096455 C CN 1096455C
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- soyasaponin
- soybean
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- soybean isoflavones
- macroporous adsorptive
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Abstract
The present invention discloses a technology for extracting soybean isoflavone and soybean saponin, which can solve the problems that soybean isoflavone and soybean saponin can not be produced in an industrialization mode and the production cost of the soybean isoflavone and soybean saponin is high. The technology comprise the following steps: step (1), whey liquid discharged by the production of soybean isolated protein is obtained; step (2), the high temperature flash evaporation and the filtration of the whey liquid are carried out; step (3), the electrodialysis of the whey liquid are carried out; step (4), the whey liquid passes through a cation exchange resin column; step (5), material liquid passes through a column of non-polar macroporous adsorptive resin; step (6), the column of non-polar macroporous adsorptive resin is washed with an organic solvent so as to obtain soybean saponin; step (7), the column of non-polar macroporous adsorptive resin is washed with an organic solvent so as to obtain soybean isoflavone. The method of the present invention cab fully use the whey liquid discharged by the production of soybean isolated protein, and can carry out large-scale industrialization production.
Description
The present invention relates to a kind of technology of extracting soybean isoflavones and Soyasaponin.
Soybean isoflavones and Soyasaponin are the secondary metabolites in the soybeans they grow, are present in soybean plumular axis and the cotyledon, all have bitterness or beany flavor.Present known soybean isoflavones has 12 kinds, comprises the glucosides of the former and nine kinds of mating types of the glycosides of 3 kinds of free types.Soyasaponin has 5 kinds.Studies show that, soybean isoflavones can be converted into the compound that is similar to hormone by bacterium in the intestines, have faint female hormone and anti-oxidant activity, in the breeding of malignant tumour, can block blood vessel hyperplasia effectively, break off the nutriment source, thereby delay or stop neoplastic disease to become cancer, can effectively suppress the generation of leukemia, colorectal carcinoma, lung cancer, cancer of the stomach, mammary cancer, prostate cancer.Simultaneously, soybean isoflavones can also be directly inhibition bone unusual again, the generation of preventing osteoporosis disease, and have antimycotic, prevent and treat effect such as hot flush.Soyasaponin has removes free radical, anti-oxidant and reduce the effect of lipid peroxide, can suppress the oxidation of lipid in the serum, reduces the content of blood cholesterol and triglyceride.Soyasaponin can also stop growth of tumour cell, suppresses the infection of hiv virus, and infected cells is had the certain protection effect.
Soybean isoflavones and Soyasaponin only account for 0.05～0.7% of soybean seeds, separation and Extraction exists many difficulties, separate soybean isoflavones and the Soyasaponin that obtains with prior art, purity is all undesirable, and because complex process, raw material consumption is huge, and cost is too high, causes the price of soybean isoflavones very expensive (price of every gram soybean isoflavones is up to 1390 dollars on the present world market).On the other hand, the whey liquid that gives off when producing soybean protein isolate at present, what have throws away after extracting soybean oligosaccharide, have in addition directly go out of use, and the soybean isoflavones and the Soyasaponin that remain in whey liquid lose thereupon, cause very big waste.
The purpose of this invention is to provide a kind of method of utilizing the whey liquid that gives off when producing soybean protein isolate to come suitability for industrialized production soybean isoflavones and Soyasaponin.
For achieving the above object, the present invention takes following design: a kind of technology of extracting soybean isoflavones and Soyasaponin may further comprise the steps:
(1) gets the production whey liquid that soybean protein isolate discharged;
(2) handle above-mentioned whey liquid with the high temperature flash distillation plant, and filter, collect filtrate with filter;
(3) above-mentioned filtrate makes the specific conductivity of feed liquid drop to 40 μ s/cm through once above electrodialysis
2Below;
(4) feed liquid is once above by cation exchange resin column, make its specific conductivity drop to 4 μ s/cm
2Below;
(5) above-mentioned feed liquid is passed through nonpolarity macroporous adsorptive resins chromatographic column;
(6) wash above-mentioned nonpolarity macroporous adsorptive resins chromatographic column with 55～65% organic solvent, collect elutriant, reclaim organic solvent by vacuum concentration after, with the products therefrom drying, promptly obtain Soyasaponin, wherein preferably methyl alcohol or ethanol of organic solvent;
(7) wash above-mentioned nonpolarity macroporous adsorptive resins chromatographic column with 90～95% organic solvent, collect elutriant, reclaim organic solvent by vacuum concentration after, with the products therefrom drying, promptly obtain soybean isoflavones, wherein preferably methyl alcohol or ethanol of organic solvent.
The present invention is owing to adopting above-mentioned design, having the following advantages:
1, the present invention is with producing the whey liquid that discharges behind the soybean protein isolate as raw material, and be to the further deep processing of whey liquid and utilize again, be a kind of engineering that turns waste into wealth, cost is low, processes precondition is provided for soybean being carried out series.
2, simple, the easy handling of technology of the present invention and required equipment, can be used for large-scale industrial production, obtain a large amount of soybean isoflavones and Soyasaponin, for soybean isoflavones and Soyasaponin in the light industry field, particularly lay a good foundation in the further development and use of pharmacy and health products trade.
3, owing to used nonpolarity macroporous adsorptive resins chromatographic column, soybean isoflavones and Soyasaponin are more fully separated out from feed liquid, be adsorbed on the resin, through the methyl alcohol or the ethanol elution of different concns, can obtain soybean isoflavones and Soyasaponin, guarantee the output capacity and the purity of product.
4, soybean isoflavones of producing with present method and Soyasaponin purity are all above 85%, and the additive or the confession that can be used as medicine fully are directly oral.
5, produce soybean isoflavones and Soyasaponin with present method, resulting byproduct can also further utilize, and cuts the waste, and reduces cost.
The invention will be further described below in conjunction with specific embodiment.
Embodiment: the processing step that extracts soybean isoflavones and Soyasaponin
One, the preparation of raw material
Producing the whey liquid that discharges behind the soybean protein isolate as the raw material that extracts soybean isoflavones and Soyasaponin, above-mentioned whey liquid comprise the whey liquid that discharges when usual way is produced soybean protein isolate and with the Production by Enzymes soybean protein isolate (this method inventor on August 3rd, 1999 apply for a patent, number of patent application is 99111244.x) whey liquid that discharged;
Two, heat denatured protein
1, by the high temperature flash distillation, temperature is brought up to 100～140 ℃ to whey liquid, and the soluble protein sex change in the whey is separated out in the high temperature flasher;
2, suitably add flocculation agent after, filter out denatured protein by filter, collect filtrate;
Three, remove inorganic salt
1, above-mentioned filtrate makes specific conductivity drop to 40 μ s/cm by twice electrodialysis
2Below;
2, feed liquid is passed through cation exchange resin column twice, make the specific conductivity of feed liquid drop to 4 μ s/cm
2Below.
Four, adopt nonpolarity macroporous adsorptive resins chromatographic column to separate soybean isoflavones and Soyasaponin
1, will remove feed liquid behind the inorganic salt by nonpolarity macroporous adsorptive resins chromatographic column (as the HP-20 resin, D370 resin etc.), the exchange volume ratio of feed liquid and resin is 50: 1 (volume ratio of feed liquid and resin is 45～55: 1 gets final product), at this moment, do not contained soybean oligosaccharide (can obtain the soybean oligosaccharide product of content more than 60%) by reverse osmosis, ultrafiltration, concentrated back spraying drying by the direct effusive liquid of resin absorption;
2, by in the feed liquid of resin absorption, contain soybean isoflavones and Soyasaponin, wash nonpolarity macroporous adsorptive resins chromatographic column with 55～65% methyl alcohol or ethanolic soln earlier, elutriant is rich in Soyasaponin;
When 3, treating elutriant face light yellow complexion, use 90～95% methyl alcohol or ethanolic soln instead and wash nonpolarity macroporous adsorptive resins chromatographic column, this elutriant is rich in soybean isoflavones;
4, two kinds of elutriant difference vacuum concentration reclaim methyl alcohol or ethanol, can obtain Soyasaponin and the 85% above soybean isoflavones of content more than 90% after the resultant product drying.
Claims (10)
1, a kind of technology of extracting soybean isoflavones and Soyasaponin, it is characterized in that: it may further comprise the steps:
(1) gets the production whey liquid that soybean protein isolate discharged;
(2) handle above-mentioned whey liquid with the high temperature flash distillation plant, and filter, collect filtrate with filter;
(3) above-mentioned filtrate makes the specific conductivity of feed liquid drop to 40 μ s/cm through once above electrodialysis
2Below;
(4) feed liquid is once above by cation exchange resin column, make its specific conductivity drop to 4 μ s/cm
2Below;
(5) above-mentioned feed liquid is passed through nonpolarity macroporous adsorptive resins chromatographic column;
(6) wash above-mentioned nonpolarity macroporous adsorptive resins chromatographic column with 55～65% organic solvent, collect elutriant, behind vacuum concentration recovery organic solvent,, promptly obtain Soyasaponin the products therefrom drying;
(7) wash above-mentioned nonpolarity macroporous adsorptive resins chromatographic column with 90～95% organic solvent, collect elutriant, behind vacuum concentration recovery organic solvent,, promptly obtain soybean isoflavones the products therefrom drying.
2, the technology of extraction soybean isoflavones according to claim 1 and Soyasaponin, it is characterized in that: described (1) produces the whey liquid that discharges behind the soybean protein isolate, comprise that usual way produces the whey liquid of soybean protein isolate discharging, or the whey liquid that discharges with the Production by Enzymes soybean protein isolate.
3, the technology of extraction soybean isoflavones according to claim 1 and Soyasaponin is characterized in that: the temperature that described (2) handle whey liquid with the high temperature flash distillation plant is 100～140 ℃.
4, according to the technology of claim 1 or 3 described extraction soybean isoflavones and Soyasaponin, it is characterized in that: after described (2) the step high temperature flash distillation, filter before, feed liquid is cooled off by water cooler.
5, the technology of extraction soybean isoflavones according to claim 1 and Soyasaponin is characterized in that: in described (3) filtrate is carried out twice electrodialysis.
6, the technology of extraction soybean isoflavones according to claim 1 and Soyasaponin is characterized in that: in described (4) feed liquid is passed through cation exchange resin column twice.
7, the technology of extraction soybean isoflavones according to claim 1 and 2 and Soyasaponin is characterized in that: when adsorbing with nonpolar macroporous adsorption resin in described (5), feed liquid and resins exchange volume ratio are 45～55: 1.
8, the technology of extraction soybean isoflavones according to claim 1 and Soyasaponin, it is characterized in that: when washing nonpolarity macroporous adsorptive resins chromatographic column with 55～65% organic solvents in described (6), treat that elutriant is little when yellow, entered for (7) step, wash nonpolarity macroporous adsorptive resins chromatographic column with 90～95% organic solvents.
9, the technology of extraction soybean isoflavones according to claim 1 and Soyasaponin is characterized in that: the organic solvent of described (6) step in order to the wash-out nonpolarity macroporous adsorptive resins chromatographic column is methyl alcohol or ethanol.
10, the technology of extraction soybean isoflavones according to claim 1 and Soyasaponin is characterized in that: the organic solvent of described (7) step in order to the wash-out nonpolarity macroporous adsorptive resins chromatographic column is methyl alcohol or ethanol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00105542A CN1096455C (en) | 2000-03-31 | 2000-03-31 | Process for extracting glycitin and soybean saponin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00105542A CN1096455C (en) | 2000-03-31 | 2000-03-31 | Process for extracting glycitin and soybean saponin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1315322A CN1315322A (en) | 2001-10-03 |
| CN1096455C true CN1096455C (en) | 2002-12-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN00105542A Expired - Fee Related CN1096455C (en) | 2000-03-31 | 2000-03-31 | Process for extracting glycitin and soybean saponin |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100452996C (en) * | 2005-04-29 | 2009-01-21 | 任发政 | Sports beverage with fatigue-releasing function |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478402B (en) * | 2013-10-15 | 2015-03-04 | 烟台东方蛋白科技有限公司 | Method for extracting pea molasses and pea protein isolate from pea starch production wastewater |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1216685A (en) * | 1997-10-31 | 1999-05-19 | 蛋白质技术国际公司 | Aglucone isoflavone enriched vegetable protein extract and isolate and process for producing |
-
2000
- 2000-03-31 CN CN00105542A patent/CN1096455C/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1216685A (en) * | 1997-10-31 | 1999-05-19 | 蛋白质技术国际公司 | Aglucone isoflavone enriched vegetable protein extract and isolate and process for producing |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100452996C (en) * | 2005-04-29 | 2009-01-21 | 任发政 | Sports beverage with fatigue-releasing function |
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| Publication number | Publication date |
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| CN1315322A (en) | 2001-10-03 |
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