CN109645222A

CN109645222A - A kind of paper mulberry biological feedstuff and its preparation method and application

Info

Publication number: CN109645222A
Application number: CN201910113079.1A
Authority: CN
Inventors: 易显凤; 林波; 庞天德; 易显菊; 丘金花; 邓素媛; 史静; 韦锦益
Original assignee: Guangxi Zhuang Autonomous Region Institute of Animal Husbandry
Current assignee: Guangxi Zhuang Autonomous Region Institute of Animal Husbandry
Priority date: 2019-02-13
Filing date: 2019-02-13
Publication date: 2019-04-19

Abstract

The present invention relates to feed processing technologies, in particular to a kind of paper mulberry biological feedstuff and its preparation method and application, biological feedstuff is by as follows at being grouped as: fermented papermulberry tree, Broussonetia papyrifera seed extract, corn, rice husk, Chinese milk vetch, dregs of beans, fermentation cassava slag, fermentation molasses and fermentation rhizoma imperatae；The application feed formula ferments the ingredients such as feed with paper-mulberry leaf, manioc waste, molasses, rhizoma imperatae, herbage ingredient can effectively be softened, the feed formula of the application, which mutually compounds, simultaneously can effectively improve feed mouthfeel, improve feed nutritive value, simultaneously, unsaturated fatty acid also rich in the application has the function of the intramuscular fat content for reducing percentage of water loss, reducing pork.

Description

A kind of paper mulberry biological feedstuff and its preparation method and application

[technical field]

The present invention relates to feed processing technologies, and in particular to a kind of paper mulberry biological feedstuff and preparation method thereof and answers With.

[background technique]

Paper mulberry (Broussonetia papyrifera) is deciduous tree, high 10-20m；Bark dark gray；Paper mulberry has Fast-growing, adaptable, distribution is wide, easily breeding, the feature that heat is high, the period of felling in turn is short.Its root system is shallow, and lateral root distribution is very wide, growth Fastly, rudiment power and tillering ability are strong, resistance to trimming.Resistance tocrocking is strong.It is distributed in temperate zone, the torrid zone of China, no matter Plain, mound Mound or mountainous region can be grown, and leaf is good pannage, and bast fiber is the high-grade materials of papermaking, and material is pure white, root Can it be used as medicine with seed, sap can control skin disease, and economic value is very high.Currently, feed with paper-mulberry leaf as feed by wide coverage, By the report of feed with paper-mulberry leaf fermenting and preparing biological feed, still, paper mulberry fermenting and preparing biological feed is merely to improve feed opening Sense, however, the ingredients such as vitamin rich in, vegetable protein in paper mulberry, however, at present also not about reduce percentage of water loss, The paper mulberry feed of intramuscular fat content is reported, for this purpose, improving the battalion of paper mulberry feed to improve the bioavailability to paper mulberry Support value, it is necessary to improve for paper mulberry biological feedstuff processing method, produce a kind of biology with high nutritive value Feed, so as to the intramuscular fat content that percentage of water loss is effectively reduced, reduces pork.

[summary of the invention]

In view of the above-mentioned problems, the technical problem to be solved in the present invention is to provide a kind of paper mulberry biological feedstuffs and preparation method thereof And application, a kind of biological feedstuff with high nutritive value is produced, so as to the flesh that percentage of water loss is effectively reduced, reduces pork Interior fat content.

In order to solve the above technical problems, The technical solution adopted by the invention is as follows:

A kind of paper mulberry biological feedstuff, the feed are made of following parts by weight ingredient: 101 parts -132 parts of fermentation paper mulberry Leaf, 31 parts -42 parts of Broussonetia papyrifera seed extract, 18 parts -32 parts of corn, 20 parts -29 parts of rice husk, 23 parts -31 parts of purple cloud English, 11 parts -23 parts of dregs of beans, 23 parts -33 parts of fermentation cassava slag, 17 parts -26 parts of fermentation molasses and 28 parts -39 parts of hair Ferment rhizoma imperatae.

Further, the Broussonetia papyrifera seed extract the preparation method comprises the following steps:

(1) ice water that the Broussonetia papyrifera seed temperature that moisture content is 3%-5% is -2~0 DEG C is impregnated, and be put into refrigerator It is refrigerated；

(2) Broussonetia papyrifera seed after step (1) being soaked carries out frying, pulverizes to obtain Broussonetia papyrifera seed powder after fried dry；

It (3) is that 1:2-3 is mixed according to solid-liquid mass ratio with organic solvent by the Broussonetia papyrifera seed powder of step (2), then It is put into supersonic extractors and carries out ultrasonic extraction；

(4) will be centrifuged after step (3) ultrasonic extraction, filter to take filtrate be put into distiller carry out distillation extract obtain paper mulberry kind Seed extract.

Further, the organic solvent are as follows: the ethyl alcohol or percentage by volume that percentage by volume is 70%-80% is The ethyl acetate of 30%-40%.

Further, the power of step (3) ultrasonic extraction is 100w-150w；Extraction time is 10min-20min.

Further, the centrifugal rotational speed of step (4) centrifugation is 1500r/min-2000r/min；Centrifugation time is 40s-60s；The vapo(u)rizing temperature of step (4) distillation is 120 DEG C -130 DEG C.

Further, the fermentation process of the fermented papermulberry tree are as follows: by feed with paper-mulberry leaf chopping with complex enzyme I and water according to quality Carry out stack retting under confined conditions than being 36-38 DEG C in temperature after mixing for 40-50:1:9-16, when stack retting a length of 48h-50h；Heap According to the inoculum concentration access activation probiotics of 4%-6% after macerating, ferment 48h- in the case where temperature is 35 DEG C -38 DEG C of ventilation condition 50h；The carry out stack retting under confined conditions for being 36-38 DEG C in temperature after ventilating fermentation；A length of 3d-4d when stack retting obtains fermentation structure Leaf；Carboxypeptidase that the complex enzyme I is 300U/mg-800U/mg by enzyme-activity unit, enzyme-activity unit 500U/mg-700U/ The pectase that the cellulase and enzyme-activity unit of mg is 1000U/mg-1500U/mg is 3-5:2-6:1 composition according to mass ratio.

Further, the fermentation process of the fermentation rhizoma imperatae are as follows: by rhizoma imperatae chopping with compound enzyme II and water according to matter For amount than being to be cooled to 60-70 DEG C of progress infusion after boiling after 30-40:1:5-10 is mixed, the infusion time is 3h-4h；After infusion It is cooled to room temperature, then according still further to the inoculum concentration access activation probiotics of 3%-5%, the air duct slats for being 33 DEG C -37 DEG C in temperature Ferment 50h-54h under part；The carry out stack retting under confined conditions for being 36-38 DEG C in temperature after ventilating fermentation；A length of 4d- when stack retting 5d obtains fermentation rhizoma imperatae；The carboxypeptidase and enzyme-activity unit that the compound enzyme II is 300U/mg-800U/mg by enzyme-activity unit For 1000U/mg-1500U/mg pectase according to mass ratio be 5-7:4-6 composition.

Further, the fermentation cassava slag the preparation method comprises the following steps: being to COD content by the inoculum concentration of 1%-3% Access activation probiotics in the manioc waste of 500mg/L-900mg/L is 0.1vvm-1.5vvm in the ventilatory capacity of air, and temperature is Aerobic fermentation 20h-24h under the conditions of 35 DEG C -38 DEG C；After aerobic fermentation temperature be 37 DEG C under the conditions of anaerobic fermentation 2d-4d, To obtain the final product.

Further, it is described fermentation molasses the preparation method comprises the following steps: being to COD content by the inoculum concentration of 1%-3% Access activation probiotics in the molasses of 1000mg/L-1500mg/L is 0.5vvm-1.8vvm in the ventilatory capacity of air, and temperature is Aerobic fermentation 20h-24h under the conditions of 35 DEG C -38 DEG C；After aerobic fermentation temperature be 37 DEG C under the conditions of anaerobic fermentation 3d-5d, To obtain the final product.

Further, the activation probiotics is 2.3 × 10 by living bacteria count⁸-4.6×10⁸The lactic acid bacteria of cfu/mg and Living bacteria count is 2.1 × 10⁸-4.6×10⁸The saccharomycete of cfu/mg is that 1-3:4-6 is mixed to prepare according to mass ratio；Institute State the activation method of lactic acid bacteria are as follows: in the fluid nutrient medium of lactic acid bacteria under anaerobic by lactobacillus inoculum, revolving speed is 500r/min-800r/min, temperature are shaking table culture 48-52h under the conditions of 37 DEG C, and the fluid nutrient medium of the lactic acid bacteria is by such as It is lower at being grouped as: yeast powder 10-15 g/L, beef extract 10-15g/L, sodium citrate 2-5g/L, sodium chloride 2-4g/L, gumbo are more Sugared 10-15g/L, manioc waste leaching liquor 20-30g/L, molasses leaching liquor 5-10g/L, the above ingredient dissolution, with sodium hydroxide tune PH to 7.5,120 DEG C of sterilizing 20min；The activation method of the saccharomycete are as follows: saccharomycete is inoculated in the Liquid Culture of saccharomycete In base under anaerobic, revolving speed is 500 r/min-800r/min, and shaking table culture 48-52h, described under the conditions of temperature is 37 DEG C The fluid nutrient medium of saccharomycete is by as follows at being grouped as: yeast powder 7-12g/L, beef extract 8-16g/L, sodium bicarbonate 3-6g/L, Sodium chloride 2-4g/L, barbaloin 15-20g/L, manioc waste leaching liquor 30-40g/L, molasses leaching liquor 10-15g/L, the above ingredient Dissolution, with sodium hydroxide tune pH to 7.0,120 DEG C of sterilizing 20min.

Present invention also provides a kind of methods for preparing paper mulberry biological feedstuff, and described method includes following steps:

(1) each raw material is weighed by the parts by weight；

(2) fermented papermulberry tree, Chinese milk vetch, corn, fermentation cassava slag, fermentation molasses and fermentation rhizoma imperatae are mixed, 35 Stack retting 2d-3d under the conditions of DEG C -37 DEG C；Then steam sterilizing 40min-50min under the conditions of 120 DEG C -130 DEG C；Then at -4 DEG C 10min is freezed under the conditions of~0 DEG C, obtains the mixture of fermentation material；

(3) rice husk and dregs of beans crushed, cross the hair for being then added to step (2) after -150 mesh screen of 100 mesh screens after mixing Then ferment material mixture sprays Broussonetia papyrifera seed extract into mixture, the water spray that or else breaks agglomerating is until mixture hand is pinched Only, under the conditions of 36 DEG C -38 DEG C stack retting 48h-50h, under the conditions of 60 DEG C -70 DEG C drying obtain the biological feedstuff of the application.

Present invention also provides a kind of paper mulberry biological feedstuffs in the purposes for reducing pork pig intramuscular fat content.

Compared with prior art, the invention has the following beneficial effects:

1, the feed with paper-mulberry leaf biological feedstuff of the application is by as follows at being grouped as: fermented papermulberry tree, Broussonetia papyrifera seed extract, jade Rice, rice husk, Chinese milk vetch, dregs of beans, fermentation cassava slag, fermentation molasses and fermentation rhizoma imperatae；It is mutually compounded between the application feed formula Feed mouthfeel can be effectively improved, improve feed nutritive value, it is more suitably applied in pork pig raising, pork can be reduced Intramuscular fat content, feed with paper-mulberry leaf can soften feed with paper-mulberry leaf after everfermentation, make feed with paper-mulberry leaf be easy to be digested and assimilated by animal, absorptivity It is higher, meanwhile, unsaturated fatty acid rich in the feed of the application can inhibit the Fatty synthesis of muscle, to reduce The intramuscular fat content of pork can be obviously improved the mouthfeel of biological feedstuff after paper mulberry fermentation, in order to provide various egg to feed It is white；The manioc waste of the application is that cassava carries out squeezing after full slag anaerobism, aerobic wastewater treatment after alcoholic fermentation being prepared Waste residue, include multiple-microorganism, microelement；Manioc waste organic matter therein after everfermentation is enriched with, so that feed Middle protein content is richer, while the cyanide content that can also be effectively reduced in manioc waste；The molasses of the application extract post-fermentation The dry matter content in feed can be improved；The application rhizoma imperatae contains the ingredients such as abundant Coixol, Cylindrin, sterol and oxalic acid, The precipitation that Trace Elements can be effectively improved after fermentation, compounds also with other compositions and feed mouthfeel can be improved.

2, containing abundant unsaturated fatty acid in the feed of the application, unsaturated fatty acid supplements feed fat, increases Add fodder energy, the feed intake of animal is caused to reduce, meanwhile, unsaturated fatty acid is also one kind of grease, can be to a certain degree Upper influence lumen fermentation can significantly reduce protein ratio to reduce animal to the feed intake of dry matter, to play reduction Percentage of water loss, reduce pork intramuscular fat content purpose.

[specific embodiment]

A specific embodiment of the invention is described further with reference to embodiments.

Embodiment 1

The processing method of the present embodiment paper mulberry biological feedstuff the following steps are included:

(1) preparation fermentation paper mulberry: in temperature after feed with paper-mulberry leaf chopping is mixed with complex enzyme I and water according to mass ratio for 40:1:9 Degree carries out stack retting for 36 DEG C under confined conditions, when stack retting a length of 48h；It is prebiotic according to 4% inoculum concentration access activation after stack retting Bacterium, ferment 48h in the case where temperature is 35 DEG C of ventilation condition；The carry out heap under confined conditions for being 36 DEG C in temperature after ventilating fermentation It macerates；A length of 3d, obtains fermented papermulberry tree when stack retting；Carboxypeptidase, the enzyme activity list that the complex enzyme I is 300U/mg by enzyme-activity unit The pectase that the cellulase and enzyme-activity unit that position is 500U/mg are 1000U/mg is 3:2:1 composition according to mass ratio.

(2) Broussonetia papyrifera seed extract extracts: the ice water that the Broussonetia papyrifera seed temperature that moisture content is 3% is -2 DEG C is impregnated, And it is put into refrigerator and is refrigerated；Broussonetia papyrifera seed after refrigeration is soaked carries out frying, pulverizes to obtain Broussonetia papyrifera seed powder after fried dry； Then according to solid-liquid mass ratio it is that 1:2 is mixed by the ethyl alcohol that Broussonetia papyrifera seed powder and percentage by volume are 70%, places into super Ultrasonic extraction is carried out in sound extractor, the power of ultrasonic extraction is 100w；Extraction time is 10min；It is centrifuged after extraction, Centrifugal rotational speed is 1500r/min；Centrifugation time, which is 40s, filters to take filtrate is put into distiller under the conditions of temperature is 120 DEG C It carries out distillation extraction and obtains Broussonetia papyrifera seed extract.

(3) prepare fermentation cassava slag: by 1% inoculum concentration, into the manioc waste that COD content is 500mg/L, access activation is beneficial Raw bacterium is 0.1vvm in the ventilatory capacity of air, and temperature is aerobic fermentation 20h under the conditions of 35 DEG C；In temperature after aerobic fermentation Under the conditions of being 37 DEG C anaerobic fermentation 2d to get.

(4) preparation fermentation molasses: by 1% inoculum concentration, into the molasses that COD content is 1000mg/L, access activation is prebiotic Bacterium is 0.5vvm in the ventilatory capacity of air, and temperature is aerobic fermentation 20h under the conditions of 35 DEG C；It is in temperature after aerobic fermentation Under the conditions of 37 DEG C anaerobic fermentation 3d to get.

(5) preparation fermentation rhizoma imperatae: after rhizoma imperatae chopping is mixed with compound enzyme II and water according to mass ratio for 30:1:5 After boiling, 60 DEG C of progress infusions are cooled to, the infusion time is 3h；Room temperature is cooled to after infusion, then the inoculation according still further to 3% Amount access activation probiotics, ferment 50h in the case where temperature is 33 DEG C of ventilation condition；It in temperature is 36 DEG C close after ventilating fermentation Stack retting is carried out under the conditions of closing；A length of 4d when stack retting obtains fermentation rhizoma imperatae；The compound enzyme II is 300U/mg by enzyme-activity unit Carboxypeptidase and enzyme-activity unit be 1000U/mg pectase according to mass ratio be 5:4 composition.

(5) each raw material: 101 parts of fermented papermulberry tree, 31 parts of Broussonetia papyrifera seed extract, 18 is weighed by by following parts by weight Corn, 20 parts of the rice husk, 23 parts of Chinese milk vetch, 11 parts of dregs of beans, 23 parts of fermentation cassava slag, 17 parts of fermentation molasses of part With 28 parts of fermentation rhizoma imperatae.

(6) fermented papermulberry tree, Chinese milk vetch, corn, fermentation cassava slag, fermentation molasses and fermentation rhizoma imperatae are mixed, 35 Stack retting 2d under the conditions of DEG C；Then steam sterilizing 40min under the conditions of 120 DEG C；Then 10min is freezed under the conditions of -4 DEG C, obtained The mixture of fermentation material；

(7) fermentation material for being then added to step (6) after mesh screen selects will be sieved with 100 mesh sieve after rice husk and dregs of beans crushing, mixing to mix Object is closed, Broussonetia papyrifera seed extract is then sprayed into mixture, or else breaks water spray until mixture hand pinches agglomerating, 36 Stack retting 48h under the conditions of DEG C, drying obtains the biological feedstuff of the application under the conditions of 60 DEG C.

Wherein, the activation probiotics of step (1) (3) (4) (5) is 2.3 × 10 by living bacteria count⁸The lactic acid bacteria of cfu/mg It is 2.1 × 10 with living bacteria count⁸The saccharomycete of cfu/mg is that 1:4 is mixed according to mass ratio.

Wherein, the activation method of lactic acid bacteria are as follows: by lactobacillus inoculum in anaerobic condition in the fluid nutrient medium of lactic acid bacteria Under, revolving speed 500r/min, temperature is shaking table culture 48h under the conditions of 37 DEG C, the fluid nutrient medium of the lactic acid bacteria by as follows at It is grouped as: yeast powder 10g/L, beef extract 10g/L, sodium citrate 2g/L, sodium chloride 2g/L, gumbo polysaccharide 10g/L, manioc waste Leaching liquor 20g/L, molasses leaching liquor 5g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.5,120 DEG C of sterilizing 20min.

Wherein, the activation method of saccharomycete are as follows: saccharomycete is inoculated in the fluid nutrient medium of saccharomycete in anaerobic condition Under, revolving speed 500r/min, temperature is shaking table culture 48h under the conditions of 37 DEG C, the fluid nutrient medium of the saccharomycete by as follows at It is grouped as: yeast powder 7g/L, beef extract 8g/L, sodium bicarbonate 3g/L, sodium chloride 2g/L, barbaloin 15g/L, manioc waste extraction 30 g/L of liquid, molasses leaching liquor 10g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.0,120 DEG C of sterilizing 20min.

The manioc waste of the present embodiment is to squeeze system after cassava carries out full slag anaerobism, aerobic wastewater treatment after alcoholic fermentation Waste residue obtained by standby, includes multiple-microorganism, microelement and a small amount of alcohol.Manioc waste leaching liquor the preparation method comprises the following steps: by wooden Potato slag is that 1:2 is mixed according to mass ratio with water, is then placed in -3 DEG C of refrigerator-freezer and freezes 8h；It is filtered after normal temperature unfreezing to room temperature Take filtrate to obtain the final product.

Then the molasses leaching liquor of the present embodiment adds gross mass the preparation method comprises the following steps: molasses and water are mixed according to 1:1 1/3 active carbon filters to take filtrate after adsorbing 2h to obtain the final product.

Embodiment 2

(1) preparation fermentation paper mulberry: by feed with paper-mulberry leaf chopping and complex enzyme I and water according to mass ratio be after 50:1:16 is mixed Temperature is 38 DEG C and carries out stack retting under confined conditions, when stack retting a length of 50h；It is prebiotic according to 6% inoculum concentration access activation after stack retting Bacterium, ferment 50h in the case where temperature is 38 DEG C of ventilation condition；The carry out heap under confined conditions for being 38 DEG C in temperature after ventilating fermentation It macerates；A length of 4d, obtains fermented papermulberry tree when stack retting；Carboxypeptidase, the enzyme activity list that the complex enzyme I is 800U/mg by enzyme-activity unit The pectase that the cellulase and enzyme-activity unit that position is 700U/mg are 1500U/mg is 5:6:1 composition according to mass ratio.

(2) Broussonetia papyrifera seed extract extracts: the Broussonetia papyrifera seed that moisture content is 5% is impregnated with the ice water that temperature is 0 DEG C, and It is put into refrigerator and is refrigerated；Broussonetia papyrifera seed after refrigeration is soaked carries out frying, pulverizes to obtain Broussonetia papyrifera seed powder after fried dry；So According to solid-liquid mass ratio it is afterwards that 1:3 is mixed by the ethyl acetate that Broussonetia papyrifera seed powder and percentage by volume are 40%, places into Ultrasonic extraction is carried out in supersonic extractors, the power of ultrasonic extraction is 150w；Extraction time is 20min；Carried out after extraction from The heart, centrifugal rotational speed 2000r/min；Centrifugation time is 60s, to filter to take filtrate to be put into distiller in temperature be 130 DEG C of conditions Under carry out distillation extract obtain Broussonetia papyrifera seed extract.

(3) prepare fermentation cassava slag: by 3% inoculum concentration, into the manioc waste that COD content is 900mg/L, access activation is beneficial Raw bacterium, air ventilatory capacity be 1.5vvm, temperature be 38 DEG C under the conditions of aerobic fermentation for 24 hours；In temperature after aerobic fermentation Under the conditions of being 37 DEG C anaerobic fermentation 4d to get.

(4) preparation fermentation molasses: by 3% inoculum concentration, into the molasses that COD content is 1500mg/L, access activation is prebiotic Bacterium, air ventilatory capacity be 1.8vvm, temperature be 38 DEG C under the conditions of aerobic fermentation for 24 hours；It is in temperature after aerobic fermentation Under the conditions of 37 DEG C anaerobic fermentation 5d to get.

(5) preparation fermentation rhizoma imperatae: after rhizoma imperatae chopping is mixed with compound enzyme II and water according to mass ratio for 40:1:10 After boiling, 70 DEG C of progress infusions are cooled to, the infusion time is 4h；Room temperature is cooled to after infusion, then the inoculation according still further to 5% Amount access activation probiotics, ferment 54h in the case where temperature is 37 DEG C of ventilation condition；It in temperature is 38 DEG C close after ventilating fermentation Stack retting is carried out under the conditions of closing；A length of 5d when stack retting obtains fermentation rhizoma imperatae；The compound enzyme II is 800U/mg by enzyme-activity unit Carboxypeptidase and enzyme-activity unit be 1500U/mg pectase according to mass ratio be 7:6 composition.

(5) each raw material: 132 parts of fermented papermulberry tree, 42 parts of Broussonetia papyrifera seed extract, 32 is weighed by by following parts by weight Corn, 29 parts of the rice husk, 31 parts of Chinese milk vetch, 23 parts of dregs of beans, 33 parts of fermentation cassava slag, 26 parts of fermentation molasses of part With 39 parts of fermentation rhizoma imperatae.

(6) fermented papermulberry tree, Chinese milk vetch, corn, fermentation cassava slag, fermentation molasses and fermentation rhizoma imperatae are mixed, 37 Stack retting 3d under the conditions of DEG C；Then steam sterilizing 50min under the conditions of 130 DEG C；Then 10min is freezed under the conditions of 0 DEG C, obtained The mixture of fermentation material；

(7) fermentation material for being then added to step (6) will be crossed after the screening of 150 mesh screens after rice husk and dregs of beans crushing, mixing to mix Object is closed, Broussonetia papyrifera seed extract is then sprayed into mixture, or else breaks water spray until mixture hand pinches agglomerating, 38 Stack retting 50h under the conditions of DEG C, drying obtains the biological feedstuff of the application under the conditions of 70 DEG C.

Wherein, the activation probiotics of step (1) (3) (4) (5) is 4.6 × 10 by living bacteria count⁸The lactic acid bacteria of cfu/mg It is 4.6 × 10 with living bacteria count⁸The saccharomycete of cfu/mg is that 3:6 is mixed according to mass ratio.

Wherein, the activation method of lactic acid bacteria are as follows: by lactobacillus inoculum in anaerobic condition in the fluid nutrient medium of lactic acid bacteria Under, revolving speed 800r/min, temperature is shaking table culture 52h under the conditions of 37 DEG C, the fluid nutrient medium of the lactic acid bacteria by as follows at It is grouped as: yeast powder 15g/L, beef extract 15g/L, sodium citrate 5g/L, sodium chloride 4g/L, gumbo polysaccharide 15g/L, manioc waste Leaching liquor 30g/L, molasses leaching liquor 10g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.5,120 DEG C of sterilizing 20min.

Wherein, the activation method of saccharomycete are as follows: saccharomycete is inoculated in the fluid nutrient medium of saccharomycete in anaerobic condition Under, revolving speed 800r/min, temperature is shaking table culture 52h under the conditions of 37 DEG C, the fluid nutrient medium of the saccharomycete by as follows at It is grouped as: yeast powder 12g/L, beef extract 16g/L, sodium bicarbonate 6g/L, sodium chloride 4g/L, barbaloin 20g/L, manioc waste leaching Extract 40g/L, molasses leaching liquor 15g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.0,120 DEG C of sterilizing 20min.

Then the molasses leaching liquor of the present embodiment adds gross mass the preparation method comprises the following steps: molasses and water are mixed according to 1:3 1/3 active carbon filters to take filtrate after adsorbing 2h to obtain the final product.

Embodiment 3

(1) preparation fermentation paper mulberry: by feed with paper-mulberry leaf chopping and complex enzyme I and water according to mass ratio be after 45:1:12 is mixed Temperature is 37 DEG C and carries out stack retting under confined conditions, when stack retting a length of 49h；It is prebiotic according to 5% inoculum concentration access activation after stack retting Bacterium, ferment 49h in the case where temperature is 37 DEG C of ventilation condition；The carry out heap under confined conditions for being 37 DEG C in temperature after ventilating fermentation It macerates；A length of 3.5d, obtains fermented papermulberry tree when stack retting；The complex enzyme I is by the carboxypeptidase that enzyme-activity unit is 500U/mg, enzyme activity The pectase that the cellulase and enzyme-activity unit that unit is 600U/mg are 1200U/mg is 4:5:1 composition according to mass ratio.

(2) Broussonetia papyrifera seed extract extracts: the ice water that the Broussonetia papyrifera seed temperature that moisture content is 4% is -1 DEG C is impregnated, And it is put into refrigerator and is refrigerated；Broussonetia papyrifera seed after refrigeration is soaked carries out frying, pulverizes to obtain Broussonetia papyrifera seed powder after fried dry； Then according to solid-liquid mass ratio it is that 1:3 is mixed by the ethyl alcohol that Broussonetia papyrifera seed powder and percentage by volume are 80%, places into super Ultrasonic extraction is carried out in sound extractor, the power of ultrasonic extraction is 120w；Extraction time is 15min；It is centrifuged after extraction, Centrifugal rotational speed is 1700r/min；Centrifugation time, which is 50s, filters to take filtrate is put into distiller under the conditions of temperature is 125 DEG C It carries out distillation extraction and obtains Broussonetia papyrifera seed extract.

(3) prepare fermentation cassava slag: by 2% inoculum concentration, into the manioc waste that COD content is 700mg/L, access activation is beneficial Raw bacterium is 0.9vvm in the ventilatory capacity of air, and temperature is aerobic fermentation 22h under the conditions of 37 DEG C；In temperature after aerobic fermentation Under the conditions of being 37 DEG C anaerobic fermentation 3d to get.

(4) preparation fermentation molasses: by 2% inoculum concentration, into the molasses that COD content is 1200mg/L, access activation is prebiotic Bacterium is 0.9vvm in the ventilatory capacity of air, and temperature is aerobic fermentation 22h under the conditions of 37 DEG C；It is in temperature after aerobic fermentation Under the conditions of 37 DEG C anaerobic fermentation 4d to get.

(5) preparation fermentation rhizoma imperatae: after rhizoma imperatae chopping is mixed with compound enzyme II and water according to mass ratio for 35:1:8 After boiling, 65 DEG C of progress infusions are cooled to, the infusion time is 3.5h；It is cooled to room temperature after infusion, is then connect according still further to 4% Kind amount access activation probiotics, ferment 52h in the case where temperature is 35 DEG C of ventilation condition；It in temperature is 37 DEG C after ventilating fermentation Stack retting is carried out under confined conditions；A length of 4.5d when stack retting obtains fermentation rhizoma imperatae；The compound enzyme II is by enzyme-activity unit The pectase that the carboxypeptidase and enzyme-activity unit of 500U/mg is 1200U/mg is 6:5 composition according to mass ratio.

(5) each raw material: 112 parts of fermented papermulberry tree, 38 parts of Broussonetia papyrifera seed extract, 22 is weighed by by following parts by weight Corn, 24 parts of the rice husk, 28 parts of Chinese milk vetch, 18 parts of dregs of beans, 27 parts of fermentation cassava slag, 21 parts of fermentation molasses of part With 32 parts of fermentation rhizoma imperatae.

(6) fermented papermulberry tree, Chinese milk vetch, corn, fermentation cassava slag, fermentation molasses and fermentation rhizoma imperatae are mixed, 36 Stack retting 2.5d under the conditions of DEG C；Then steam sterilizing 45min under the conditions of 125 DEG C；Then 10min is freezed under the conditions of -2 DEG C, obtained To the mixture of fermentation material；

(7) fermentation material for being then added to step (6) will be crossed after the screening of 120 mesh screens after rice husk and dregs of beans crushing, mixing to mix Object is closed, Broussonetia papyrifera seed extract is then sprayed into mixture, or else breaks water spray until mixture hand pinches agglomerating, 37 Stack retting 49h under the conditions of DEG C, drying obtains the biological feedstuff of the application under the conditions of 65 DEG C.

Wherein, the activation probiotics of step (1) (3) (4) (5) is 3.6 × 10 by living bacteria count⁸The lactic acid bacteria of cfu/mg It is 3.1 × 10 with living bacteria count⁸The saccharomycete of cfu/mg is that 2:5 is mixed according to mass ratio.

Wherein, the activation method of lactic acid bacteria are as follows: by lactobacillus inoculum in anaerobic condition in the fluid nutrient medium of lactic acid bacteria Under, revolving speed 700r/min, temperature is shaking table culture 50h under the conditions of 37 DEG C, the fluid nutrient medium of the lactic acid bacteria by as follows at It is grouped as: yeast powder 12g/L, beef extract 12g/L, sodium citrate 4g/L, sodium chloride 3g/L, gumbo polysaccharide 13g/L, manioc waste Leaching liquor 25g/L, molasses leaching liquor 8g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.5,120 DEG C of sterilizing 20min.

Wherein, the activation method of saccharomycete are as follows: saccharomycete is inoculated in the fluid nutrient medium of saccharomycete in anaerobic condition Under, revolving speed 700r/min, temperature is shaking table culture 50h under the conditions of 37 DEG C, the fluid nutrient medium of the saccharomycete by as follows at It is grouped as: yeast powder 10g/L, beef extract 11g/L, sodium bicarbonate 5g/L, sodium chloride 3g/L, barbaloin 18g/L, manioc waste leaching Extract 35g/L, molasses leaching liquor 13g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.0,120 DEG C of sterilizing 20min.

Then the molasses leaching liquor of the present embodiment adds gross mass the preparation method comprises the following steps: molasses and water are mixed according to 1:2 1/3 active carbon filters to take filtrate after adsorbing 2h to obtain the final product.

Control group 1:

Fermented papermulberry tree is free of in the biological feedstuff raw material of this control group, raw material comprises the following components in parts by weight: 31 parts of Broussonetia papyrifera seed extract, 18 parts of corn, 20 parts of rice husk, 23 parts of Chinese milk vetch, 11 parts of dregs of beans, 23 parts of hair Ferment manioc waste, 17 parts of fermentation molasses and 28 parts of fermentation rhizoma imperatae, other embodiment and embodiment 1 are completely the same.

Control group 2:

Fermentation cassava slag is free of in the biological feedstuff raw material of this control group, raw material comprises the following components in parts by weight: 101 parts of fermented papermulberry tree, 31 parts of Broussonetia papyrifera seed extract, 18 parts of corn, 20 parts of rice husk, 23 parts of Chinese milk vetch, 11 Part dregs of beans, 17 parts of fermentation molasses and 28 parts of fermentation rhizoma imperatae, other embodiment and embodiment 1 it is completely the same.

Control group 3:

Without fermentation molasses in the biological feedstuff raw material of this control group, raw material is comprised the following components in parts by weight: 101 Fermented papermulberry tree, 31 parts of the Broussonetia papyrifera seed extract, 18 parts of corn, 20 parts of rice husk, 23 parts of Chinese milk vetch, 11 parts of part Dregs of beans, 23 parts of fermentation cassava slag and 28 parts of fermentation rhizoma imperatae, other embodiment and embodiment 1 are completely the same.

Control group 4:

Without fermentation rhizoma imperatae in the biological feedstuff raw material of this control group, raw material is comprised the following components in parts by weight: 101 parts of fermented papermulberry tree, 31 parts of Broussonetia papyrifera seed extract, 18 parts of corn, 20 parts of rice husk, 23 parts of Chinese milk vetch, 11 The dregs of beans, 23 parts of fermentation cassava slag and 17 parts of fermentation molasses of part, other embodiment and embodiment 1 are completely the same.

Control group 5:

Broussonetia papyrifera seed extract is free of in the biological feedstuff raw material of this control group, raw material is by following parts by weight at grouping At: 101 parts of fermented papermulberry tree, 18 parts of corn, 20 parts of rice husk, 23 parts of Chinese milk vetch, 11 parts of dregs of beans, 23 parts of hair Ferment manioc waste, 17 parts of fermentation molasses and 28 parts of fermentation rhizoma imperatae, other embodiment and embodiment 1 are completely the same.

Control group 6:

The feed with paper-mulberry leaf of this control group, manioc waste, molasses, rhizoma imperatae are without fermentation process, and Broussonetia papyrifera seed is without mentioning It takes, other preparation methods and embodiment 1 are completely the same, and biological feedstuff formula comprises the following components in parts by weight: 101 parts Feed with paper-mulberry leaf, 31 parts of Broussonetia papyrifera seed, 18 parts of corn, 20 parts of rice husk, 23 parts of Chinese milk vetch, 11 parts of dregs of beans, 23 parts of wood Potato slag, 17 parts of molasses and 28 parts of rhizoma imperatae.

Test experiments 1: the determination of bioactive constituent of biological feedstuff:

Cyanide content (cyaniding analyte detection CN in testing example 1-3 and control group 1-6 biological feedstuff^-Content), thick egg Bai Hanliang, crude fiber content, dry matter content, total amino acid content and unsaturated fatty acid content, are specifically shown in Table 1:

Table 1

As seen from the above table, the cyanide content of embodiment 1-3 is significantly lower than control group 6 and 5 phase of control group 2 and control group When；Illustrate, mainly from manioc waste, explanation can effectively be lowered in feed the cyanide of the application with the fermentation process of the application Cyanide content, and the compounding of other ingredients can more or less reduce cyanide content；The crude protein of embodiment 1-3, total ammonia Base acid content is apparently higher than control group 6, illustrates, the crude protein and total amino acid content of the application feed are matched with the application feed Fang Xiangguan, each ingredient is indispensable, and the fermentation process of the application can effectively improve feed crude protein and total amino acid content； The crude fiber content of embodiment 1-3 is significantly lower than control group 6, illustrates, the fermentation process and feed formula of the application can reduce life The coarse fiber content of object feed；The dry matter content of embodiment 1-3 be greater than control group 6, illustrate, the processing method of the application with Dry matter content can be improved in feed formula；The unsaturated fatty acid content of embodiment 1-3 and control group 1-4 are apparently higher than control Group 5-6 illustrates that the Broussonetia papyrifera seed extract addition of the application can effectively improve the unsaturated fatty acid content in feed.

Testing experiment 2: sensory evaluation:

The subjective appreciation person that sensory evaluation scores method is made of 10 feed processing related technical personnel, to different disposal sample Sensory evaluation is carried out, evaluation result is shown in Table 2:

Table 2

As seen from the above table, the smell of control group 2-3 and control group 6 is not so good as other group, illustrates the manioc waste fermentation of the application With the molasses fermented palatability and quality for effectively improving feed.

Testing experiment 3: viable count after probiotics activation

Control group A:

The probiotics of this control group directlys adopt commercially available lactic acid bacteria and saccharomycete prepares biological feedstuff without overactivation, Other preparation methods are identical with embodiment 1.

Control group B:

The lactic acid bacteria of this control group, saccharomycete activation medium do not add manioc waste leaching liquor, i.e. the liquid training of lactic acid bacteria Base is supported by as follows at being grouped as: yeast powder 10g/L, beef extract 10g/L, sodium citrate 2g/L, sodium chloride 2g/L, gumbo polysaccharide 10g/L, molasses leaching liquor 5g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.5,120 DEG C of sterilizing 20min.Saccharomycete Fluid nutrient medium by as follows at being grouped as: yeast powder 7g/L, beef extract 8g/L, sodium bicarbonate 3g/L, sodium chloride 2g/L, reed Luxuriant growth glucoside 15g/L, molasses leaching liquor 10g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.0,120 DEG C of sterilizing 20min. Other preparation methods are identical with embodiment 1.

Control group C:

The lactic acid bacteria of this control group, saccharomycete activation medium do not add molasses leaching liquor, the i.e. Liquid Culture of lactic acid bacteria Base is by as follows at being grouped as: yeast powder 10g/L, beef extract 10g/L, sodium citrate 2g/L, sodium chloride 2g/L, gumbo polysaccharide 10g/L, manioc waste leaching liquor 20g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.5,120 DEG C of sterilizing 20min.Yeast The fluid nutrient medium of bacterium is by as follows at being grouped as: yeast powder 7g/L, beef extract 8g/L, sodium bicarbonate 3g/L, sodium chloride 2g/L, Barbaloin 15g/L, manioc waste leaching liquor 30g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.0,120 DEG C of sterilizings 20 min.Other preparation methods are identical with embodiment 1.

Control group D:

The lactic acid bacteria of this control group, saccharomycete activation medium use glucose substitution gumbo polysaccharide or barbaloin, i.e. cream The fluid nutrient medium of sour bacterium is by as follows at being grouped as: i.e. the fluid nutrient medium of lactic acid bacteria is by as follows at being grouped as: yeast powder 10g/ L, beef extract 10g/L, sodium citrate 2g/L, sodium chloride 2g/L, glucose 10g/L, manioc waste leaching liquor 20g/L, molasses extraction Liquid 5g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.5,120 DEG C of sterilizing 20min.The fluid nutrient medium of saccharomycete by As follows at being grouped as: yeast powder 7g/L, beef extract 8g/L, sodium bicarbonate 3g/L, sodium chloride 2g/L, glucose 15g/L, cassava Slag leaching liquor 30g/L, molasses leaching liquor 10g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.0,120 DEG C of sterilizings 20min.Other preparation methods are identical with embodiment 1.

The living bacteria count of testing example 1 and control group A-D finished product feedstuff, is specifically shown in Table 3:

Table 3

As seen from the above table, the living bacteria count amount of embodiment 1 is apparently higher than the benefit that control group A-control group D illustrates the application Raw bacterium activation method can effectively improve the living bacteria count of biological feedstuff.

Feeding experiment:

Piglet grows into 100kg from 20kg and is known as the stage of fattening；

It is carried out at the test site of this institute, test selection age, the miscellaneous market pig of the consistent ternary of weight are divided into 8 groups, every group 10 Head, experimental stage are to deliver for sale after 20kg is fattened to 110kg.

Feeding is carried out by pig of the feed formula of test group 1-7 and control group to the stage of fattening, daily to feed 3 times, morning 6: 00-7:00, noon 12:00-13:00, afternoon 18:00-19:00；Other management methods are consistent.

Test group 1: it is fed with pig of the feed of embodiment 1 to the stage of fattening；

Test group 2: it is fed with pig of the feed of control group 1 to the stage of fattening；

Test group 3: it is fed with pig of the feed of control group 2 to the stage of fattening；

Test group 4: it is fed with pig of the feed of control group 3 to the stage of fattening；

Test group 5: it is fed with pig of the feed of control group 4 to the stage of fattening；

Test group 6: it is fed with pig of the feed of control group 5 to the stage of fattening；

Test group 7: it is fed with pig of the feed of control group 6 to the stage of fattening；

Control group: it is fed with pig of the corn flour to the stage of fattening.

Test method and testing index

The acquisition of sample: in off-test, 3 for testing end of term live-weight close to its cell mean are selected respectively from each group Pig is butchered after on an empty stomach 24, measures the pH value, percentage of water loss and intramuscular fat content of pork by pig:

PH value: longissimus dorsi muscle acanthopore measurement of the pH meter directly at trunk reciprocal the with thoracic vertebrae is used；

Percentage of water loss: cutting the thin slice of thick 1.0cm at the 1st lumbar vertebrae longissimus dorsi muscle, is flat on clean rubber tissue, then with directly The rounded sample device of diameter 2.5cm cuts centre meat sample, after weighing.It is each up and down to pad 18 layers between meat sample is placed in two layers of gauze Qualitative Medium speed filter paper.One piece of writing hard plastic board is respectively padded outside filter paper, being then placed into soil allows to add on bulbs of pressure meter It is depressed into 35kg, 5min is kept, weighs meat sample weight immediately after removing pressure.Percentage of water loss adds meat sample weight after pressure to add meat sample weight before pressure.

Calculation formula: percentage of water loss (%)=plus meat sample weight after pressure+plus meat sample weight before pressure × 100%.

The measurement of intramuscular fat: soxhlet extraction methods are used, the meat sample dry matter of 2.0g or so are taken, with fat-free filter paper and degreasing Cotton thread wraps up meat sample, makees solvent with acetic acid, on soxhlet type instrument, extracts hour, acquires fat using fatty bottle incrementss and contain Amount.

Specifically it is shown in Table 4:

Table 4

Group	PH value	Percentage of water loss (%)	Intramuscular fat content (%)
				Test group 1	6.13	1.35	1.02
Test group 2	6.21	1.54	3.24
				Test group 3	6.06	1.63	2.15
Test group 4	6.31	1.46	2.08
				Test group 5	6.41	2.34	4.16
Test group 6	6.25	3.15	5.03
				Test group 7	6.12	3.47	5.13
Control group	5.47	4.06	6.17

As seen from the above table, for pH value, test group 1-7 is close, is higher than control group, illustrates to compare corn flour using greenfeed So that pork pH is higher；The percentage of water loss of test group 1-4 is close, is less than test group 5-7 and control group, illustrates the feed of the application The water-retaining property of pork can be improved in raising；It is fermented papermulberry tree, fermentation cassava slag, fermentation on the ingredient that percentage of water loss influences in formula Molasses；The intramuscular fat content of test group is less than test group 2-7 and control group；Illustrate that the feed formula of the application can reduce pig The intramuscular fat content of meat.

In conclusion the feed formula and preparation method of the application can reduce cyanide content in feed, improve crude protein Content reduces crude fiber content, improves dry matter, total amino acid and unsaturated fatty acid content；It can using the feed of the application Percentage of water loss, the purpose of intramuscular fat content is effectively reduced.

Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair Bright patent claim, it is all the present invention suggested by technical spirit under completed same changes or modifications change, should all belong to In the covered the scope of the patents of the present invention.

Claims

1. a kind of paper mulberry biological feedstuff, which is characterized in that the feed is made of following parts by weight ingredient: 101 parts -132 parts Fermented papermulberry tree, 31 parts -42 parts of Broussonetia papyrifera seed extract, 18 parts -32 parts of corn, 20 parts -29 parts of rice husk, 23 part -31 Part Chinese milk vetch, 11 parts -23 parts of dregs of beans, 23 parts -33 parts of fermentation cassava slag, 17 parts -26 parts of fermentation molasses and 28 part -39 The fermentation rhizoma imperatae of part.

2. a kind of paper mulberry biological feedstuff according to claim 1, which is characterized in that the preparation of the Broussonetia papyrifera seed extract Method are as follows:

(1) ice water that the Broussonetia papyrifera seed temperature that moisture content is 3%-5% is -2~0 DEG C is impregnated, and is put into refrigerator and carries out Refrigeration；

(3) it is that 1:2-3 is mixed according to solid-liquid mass ratio with organic solvent by the Broussonetia papyrifera seed powder of step (2), is then placed in Ultrasonic extraction is carried out in supersonic extractors；

(4) it will be centrifuged after step (3) ultrasonic extraction, filter to take filtrate and be put into distiller and carry out distillation and extract obtaining Broussonetia papyrifera seed and mentioning Take object.

3. a kind of paper mulberry biological feedstuff according to claim 2, which is characterized in that the organic solvent are as follows: volume basis The ethyl acetate that the ethyl alcohol or percentage by volume that number is 70%-80% are 30%-40%.

4. a kind of paper mulberry biological feedstuff according to claim 2, which is characterized in that the function of step (3) ultrasonic extraction Rate is 100w-150w；Extraction time is 10min-20min.

5. a kind of paper mulberry biological feedstuff according to claim 2, which is characterized in that the centrifugation of step (4) centrifugation turns Speed is 1500r/min-2000r/min；Centrifugation time is 40s-60s；The vapo(u)rizing temperature of the step (4) distillation is 120 DEG C- 130℃。

6. a kind of paper mulberry biological feedstuff according to claim 1, which is characterized in that the fermentation process of the fermented papermulberry tree Are as follows: it be after 40-50:1:9-16 mixing according to mass ratio by feed with paper-mulberry leaf chopping and complex enzyme I and water in temperature is 36-38 DEG C closed Under the conditions of carry out stack retting, when stack retting a length of 48h-50h；According to the inoculum concentration access activation probiotics of 4%-6% after stack retting, in temperature Degree is the 48h-50h that ferments under 35 DEG C -38 DEG C of ventilation condition；After ventilating fermentation temperature be 36-38 DEG C under confined conditions into Row stack retting；A length of 3d-4d, obtains fermented papermulberry tree when stack retting；The complex enzyme I is 300U/mg-800U/mg by enzyme-activity unit Carboxypeptidase, enzyme-activity unit be 500U/mg-700U/mg cellulase and enzyme-activity unit be 1000U/mg-1500U/mg fruit Glue enzyme is 3-5:2-6:1 composition according to mass ratio.

7. a kind of paper mulberry biological feedstuff according to claim 1, which is characterized in that the fermentation process of the fermentation rhizoma imperatae Are as follows: after boiling after mixing rhizoma imperatae chopping for 30-40:1:5-10 according to mass ratio with compound enzyme II and water, it is cooled to 60-70 DEG C carry out infusion, the infusion time be 3h-4h；It is cooled to room temperature after infusion, then accesses activation according still further to the inoculum concentration of 3%-5% Probiotics, ferment 50h-54h in the case where temperature is 33 DEG C -37 DEG C of ventilation condition；It in temperature is 36-38 DEG C close after ventilating fermentation Stack retting is carried out under the conditions of closing；A length of 4d-5d when stack retting obtains fermentation rhizoma imperatae；The compound enzyme II is 300U/ by enzyme-activity unit The pectase that the carboxypeptidase and enzyme-activity unit of mg-800U/mg is 1000U/mg-1500U/mg is 5-7:4-6 group according to mass ratio At.

8. a kind of paper mulberry biological feedstuff according to claim 6-7 any one, which is characterized in that the activation probiotics by Living bacteria count is 2.3 × 10⁸-4.6×10⁸The lactic acid bacteria of cfu/mg and living bacteria count are 2.1 × 10⁸-4.6×10⁸cfu/ The saccharomycete of mg is that 1-3:4-6 is mixed to prepare according to mass ratio；The activation method of the lactic acid bacteria are as follows: by lactobacillus inoculum In the fluid nutrient medium of lactic acid bacteria under anaerobic, revolving speed 500r/min-800r/min, temperature are shaken under the conditions of being 37 DEG C Bed culture 48-52h, the fluid nutrient medium of the lactic acid bacteria is by as follows at being grouped as: yeast powder 10-15g/L, beef extract 10- 15g/L, sodium citrate 2-5g/L, sodium chloride 2-4g/L, gumbo polysaccharide 10-15g/L, manioc waste leaching liquor 20-30g/L, molasses Leaching liquor 5-10g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.5,120 DEG C of sterilizing 20min；The work of the saccharomycete Change method are as follows: be inoculated in saccharomycete in the fluid nutrient medium of saccharomycete under anaerobic, revolving speed 500r/min-800r/ Min, temperature are shaking table culture 48-52h under the conditions of 37 DEG C, and the fluid nutrient medium of the saccharomycete is by as follows at being grouped as: yeast Powder 7-12g/L, beef extract 8-16g/L, sodium bicarbonate 3-6g/L, sodium chloride 2-4g/L, barbaloin 15-20g/L, manioc waste extraction Liquid 30-40g/L, molasses leaching liquor 10-15g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.0,120 DEG C of sterilizings 20min。

9. the method for any one of the claim 1-8 preparation paper mulberry biological feedstuff, which is characterized in that the method includes as follows Step:

(1) each raw material is weighed by the parts by weight；

(2) fermented papermulberry tree, Chinese milk vetch, corn, fermentation cassava slag, fermentation molasses and fermentation rhizoma imperatae are mixed, at 35 DEG C -37 Stack retting 2d-3d under the conditions of DEG C；Then steam sterilizing 40min-50min under the conditions of 120 DEG C -130 DEG C；Then at -4 DEG C~0 DEG C Under the conditions of freeze 10min, obtain the mixture of fermentation material；

(3) rice husk and dregs of beans crushed, cross the fermentation material for being then added to step (2) after -150 mesh screen of 100 mesh screens after mixing Then mixture sprays Broussonetia papyrifera seed extract into mixture, or else break water spray until mixture hand pinches agglomerating, 36 Stack retting 48h-50h under the conditions of DEG C -38 DEG C, drying obtains the biological feedstuff of the application under the conditions of 60 DEG C -70 DEG C.

10. the paper mulberry biological feedstuff of preparation method described in the paper mulberry biological feedstuff or claim 9 of any one of claim 1-8 exists Reduce the purposes of pork pig intramuscular fat content.