CN109644940A - A kind of sheep Escherichia coli attack malicious method - Google Patents
A kind of sheep Escherichia coli attack malicious method Download PDFInfo
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- CN109644940A CN109644940A CN201910119205.4A CN201910119205A CN109644940A CN 109644940 A CN109644940 A CN 109644940A CN 201910119205 A CN201910119205 A CN 201910119205A CN 109644940 A CN109644940 A CN 109644940A
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- Prior art keywords
- lamb
- escherichia coli
- malicious
- sheep
- test
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- 241000588724 Escherichia coli Species 0.000 title claims abstract description 28
- 241001494479 Pecora Species 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 18
- 235000019687 Lamb Nutrition 0.000 claims abstract description 45
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 7
- 206010012735 Diarrhoea Diseases 0.000 claims description 7
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 230000003115 biocidal effect Effects 0.000 claims description 2
- 201000006549 dyspepsia Diseases 0.000 claims description 2
- 239000004615 ingredient Substances 0.000 claims description 2
- 208000001848 dysentery Diseases 0.000 claims 1
- 206010016256 fatigue Diseases 0.000 claims 1
- 210000004251 human milk Anatomy 0.000 claims 1
- 235000020256 human milk Nutrition 0.000 claims 1
- 239000002574 poison Substances 0.000 claims 1
- 231100000614 poison Toxicity 0.000 claims 1
- 230000000384 rearing effect Effects 0.000 claims 1
- 241000894006 Bacteria Species 0.000 abstract description 10
- 239000008188 pellet Substances 0.000 abstract description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 abstract description 2
- 239000012895 dilution Substances 0.000 description 5
- 238000010790 dilution Methods 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 206010004016 Bacterial diarrhoea Diseases 0.000 description 1
- 201000004569 Blindness Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000010422 painting Methods 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Malicious method is attacked the invention discloses a kind of sheep Escherichia coli, comprising the following steps: test lamb is divided into healthy control group and challenge test group；The malicious first stage is attacked to the feeding of test lamb for foodstuff, draws Escherichia coli Growth curve；It attacks malicious second stage and F17 Escherichia coli bacteria liquid is fed with syringe to test lamb；The lamb state of observation in every 6 hours records lamb pellet morphology, and adjusts Escherichia coli bacteria liquid dosage according to lamb physiology combinations of states growth curve of bacteria；3 d are continuously fed until lamb physiological status remains unchanged, are recorded with " normal " (1 type, 2 types), " paste " (3 type), " water sample " (4 type) three kinds of excrement stickiness for standard.A kind of sheep Escherichia coli proposed by the present invention attack malicious method, and targetedly improve sheep Escherichia coli attacks malicious success rate.
Description
Technical field
Malicious method is attacked the present invention relates to animal and veterinary technical field more particularly to a kind of sheep Escherichia coli.
Background technique
In the intensive culture production process of modernization, bacterial diarrhea is most commonly seen high-incidence in intensive sheep farm, carefully
The therapeutic scheme of bacterium property diarrhea is there are many defects, and conventional bacteria attacks malicious technology, and there is blindness, low efficiency and specific aims
The disadvantages of not strong.
Summary of the invention
What the main object of the present invention was to provide a kind of sheep Escherichia coli attacks malicious method, it is intended to solve traditional bacterium and attack
Low efficiency existing for malicious method, the disadvantages of specific aim is not strong.
In order to achieve the goal above, attacking malicious method the invention proposes a kind of sheep Escherichia coli, which is characterized in that packet
Include following steps:
Step 1 draws Escherichia coli Growth curve, and malicious bacterial concentration is attacked in adjustment；
Test lamb is divided into healthy control group and challenge test group by step 2；
Step 3 attacks the malicious first stage, to test lamb feeding for foodstuff；
Step 4 attacks malicious second stage, makes to test the oral F17 Escherichia coli bacteria liquid of lamb；
Step 5 attacks malicious second stage, and the lamb state of observation in every 6 hours records lamb physiological status；
Step 6 attacks malicious second stage, continuously feeds 5d until lamb physiological status remains unchanged, with " normal ", " paste
Shape ", " water sample " three kinds of excrement stickiness are that standard is recorded.
The step 2 includes: that test lamb is divided into healthy control group and challenge test group, is strictly isolated between two groups
Raising is organized interior lamb subfield and is individually raised.
The step 3 includes: to attack the malicious first stage, to the feeding of test lamb for foodstuff, the physiology of observation test lamb
State can carry out the operation for attacking malicious second stage if continuous 2 days do not occur diarrhea phenomenon at this time.
The step 6 include: continuously feed 3d until lamb physiological status remain unchanged, with " normal ", " paste ",
" water sample " three kinds of excrement stickiness are that standard is recorded；" normal " includes 1 type, 2 types, " paste " corresponding 3 types；" water sample " is corresponding
4 types.
In step 4, every test lamb receive daily the mean dose concentration of F17 coli strain be 4.6 ×
108CFU·mL-1；Mean dose concentration need to be adjusted according to growth curve.
Compared with the existing technology, the case where the invention has the benefit that according to feeding for after foodstuff, excludes to be eaten by generation
Diarrhea caused by expecting also provides the standard of perfection of pellet morphology, targetedly provides for the malicious scheme of attacking of sheep Escherichia coli
With reference to.
Detailed description of the invention
Fig. 1 is Escherichia coli Growth curve graph.
Specific embodiment
Below by way of specific embodiment, invention is further described in detail.
The present invention proposes a kind of malicious method of attacking of sheep Escherichia coli, the sheep Escherichia coli attack malicious method include with
Lower step:
Step 1 draws Escherichia coli Growth curve, and malicious bacterial concentration is attacked in adjustment；Growth curve is shown in Fig. 1.
Growth curve the production method is as follows:
(1), plate streaking, picking single colonie are inoculated with 5mL LB culture medium, and 37 DEG C are incubated overnight；
(2), take 1mL seed liquor that 100mL LB culture medium is respectively added, 37 DEG C are incubated overnight；
(3), 1mL bacterium solution is taken within every 1 hour, 3 repetitions measure OD600Light absorption value.
Bacterial Plate bacterium colony counting operation method is as follows:
(1), Escherichia coli bacteria liquid to be measured is inoculated with to LB culture medium, is incubated overnight.Measure OD600Light absorption value takes a certain amount of
The bacteria suspension of (such as 100 μ l) carries out 10 × times gradient series dilution；
(2), a certain amount of (such as 100 μ l) dilution is taken to be spread evenly across plating medium, 3 repetitions of each dilution, 37
It DEG C is incubated overnight；
(3), after growing bacterium colony on plate, choosing the most suitable plate of dilution, (usual clump count is tens of to hundreds of
Between a), the clump count on plate is counted, according to the amount of liquid of dilution and painting plate, converses the viable count in original bacterium solution.
Usual unit is CUF/ml, i.e., how many viable count in every original bacterium solution of ml.
Test lamb is divided into healthy control group and challenge test group by step 2；
Step 2 includes: to choose 18 3-5 ages in days, be in a good state of health, the individual of lamb similar in weight, will test lamb
It is divided into healthy control group and challenge test group, wherein blank control group 3, challenge test group 15.Strictly it is isolated between two groups
Raising is organized interior lamb subfield and is individually raised；
Step 3 attacks the malicious first stage, to the feeding of test lamb for foodstuff, is free of antibiotic and microorganism for foodstuff ingredient
Preparation.
Step 3 includes: to attack the malicious first stage, and to the feeding of test lamb for foodstuff, the physiological status of lamb is tested in observation, if
Do not occur diarrhea phenomenon within continuous 2 days, can exclude as causing to test endocrine disturbance caused by lamb indigestion for foodstuff, this
When can carry out attacking malicious second stage；
Step 4 attacks malicious second stage, makes to test the oral F17 Escherichia coli bacteria liquid of lamb；
Step 4 include: every test lamb receive daily the mean dose concentration of F17 coli strain be 4.6 ×
108CFU·mL-1；Mean dose concentration need to be adjusted according to growth curve.
Step 5 attacks malicious second stage, and the lamb state of observation in every 6 hours records lamb physiological status；
Step 6 attacks malicious second stage, continuously feed 3d until lamb physiological status remain unchanged, with " normal " (1 type, 2
Type), " paste " (3 type), " water sample " (4 type) three kinds of excrement stickiness be standard recorded.Excrement stickiness standard is shown in Table 1.
1 excrement classification chart of table
The above display describes basic principles and main features and advantage of the invention.The technical staff of the industry should
Understand, the present invention is not limited to the above embodiments, what is described in the above embodiment and the description is only saying the principle of the present invention,
Without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes and improvements are all
It drops into the claimed scope of the invention.The scope of the present invention is defined by the appended claims and its equivalents.
Claims (6)
1. a kind of sheep Escherichia coli attack malicious method, which comprises the following steps:
Step 1 draws Escherichia coli Growth curve, and malicious bacterial concentration is attacked in adjustment；
Test lamb is divided into healthy control group and challenge test group by step 2；
Step 3 attacks the malicious first stage, to test lamb feeding for foodstuff；
Step 4 attacks malicious second stage, makes to test the oral F17 Escherichia coli bacteria liquid of lamb；
Step 5 attacks malicious second stage, and the lamb state of observation in every 6 hours records lamb physiological status；
Step 6 attacks malicious second stage, continuously feeds 5d until lamb physiological status remains unchanged, with " normal ", " paste ", " water
Three kinds of excrement stickiness of sample " are that standard is recorded.
2. sheep Escherichia coli as described in claim 1 attack malicious method, which is characterized in that the step 2 includes: that will try
It tests lamb and is divided into healthy control group and challenge test group, stringent isolated rearing between two groups is organized interior lamb subfield and individually raised.
3. sheep Escherichia coli as described in claim 1 attack malicious method, which is characterized in that the step 3 includes: to attack poison
First stage, to the feeding of test lamb for foodstuff, the physiological status of lamb is tested in observation, if continuous 2 days do not occur diarrhea phenomenon,
It can exclude to lead to diarrhea caused by lamb indigestion as substitute breast milk, challenge test can be carried out at this time；Wherein, physiological status packet
Include and be not limited to dysentery, watery diarrhea, lassitude.
4. sheep Escherichia coli as described in claim 1 attack malicious method, which is characterized in that the step 6 includes: continuous
3d is fed until lamb physiological status remains unchanged, using " normal ", " paste ", " water sample " three kinds of excrement stickiness as standard progress
Record；" normal " includes 1 type and 2 types, " paste " corresponding 3 types；" water sample " corresponds to 4 types；
。
5. sheep Escherichia coli as described in claim 1 attack malicious method, which is characterized in that do not include antibiosis for foodstuff ingredient
Element.
6. sheep Escherichia coli as described in claim 1 attack malicious method, which is characterized in that in step 4, every test lamb
Receiving the mean dose concentration of F17 coli strain daily is 4.6 × 108CFU·mL-1；Mean dose concentration need to be according to life
Long curve is adjusted.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910119205.4A CN109644940A (en) | 2019-02-18 | 2019-02-18 | A kind of sheep Escherichia coli attack malicious method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910119205.4A CN109644940A (en) | 2019-02-18 | 2019-02-18 | A kind of sheep Escherichia coli attack malicious method |
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| Publication Number | Publication Date |
|---|---|
| CN109644940A true CN109644940A (en) | 2019-04-19 |
Family
ID=66122890
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910119205.4A Pending CN109644940A (en) | 2019-02-18 | 2019-02-18 | A kind of sheep Escherichia coli attack malicious method |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6355859B1 (en) * | 1997-05-20 | 2002-03-12 | Biotechnology Research And Development Corporation | Interactions between genotype and diet in swine that prevent E. coli associated intestinal disease |
| US20090053793A1 (en) * | 2006-10-13 | 2009-02-26 | Rowan University | Ethanol resistant and furfural resistant strains of E. coli FBR5 for production of ethanol from cellulosic biomass |
| WO2014033313A1 (en) * | 2012-09-03 | 2014-03-06 | Vib Vzw | Protective anti-etec antibody |
| CN104286522A (en) * | 2014-10-01 | 2015-01-21 | 姜海艳 | Formula of sheep feed and preparation method of sheep feed |
| CN105920619A (en) * | 2016-05-25 | 2016-09-07 | 天津市畜牧兽医研究所 | Method for building weaned-piglet inflammatory response model and application |
| CN106337080A (en) * | 2016-11-29 | 2017-01-18 | 河南科技大学 | Swine escherichia coli pathogenicity test method |
| US20170333496A1 (en) * | 2016-05-20 | 2017-11-23 | Church & Dwight Co., Inc. | Lactobacillus and bacillus based direct fed microbial treatment for poultry and method of use |
| CN108018228A (en) * | 2017-09-28 | 2018-05-11 | 江苏省农业科学院 | One plant of Escherichia coli O 157:H7 low virulent strains and its application |
| CN108060098A (en) * | 2017-12-13 | 2018-05-22 | 山东大学 | A kind of fermentation culture method of Escherichia coli and its application in feed addictive |
-
2019
- 2019-02-18 CN CN201910119205.4A patent/CN109644940A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6355859B1 (en) * | 1997-05-20 | 2002-03-12 | Biotechnology Research And Development Corporation | Interactions between genotype and diet in swine that prevent E. coli associated intestinal disease |
| US20090053793A1 (en) * | 2006-10-13 | 2009-02-26 | Rowan University | Ethanol resistant and furfural resistant strains of E. coli FBR5 for production of ethanol from cellulosic biomass |
| WO2014033313A1 (en) * | 2012-09-03 | 2014-03-06 | Vib Vzw | Protective anti-etec antibody |
| CN104286522A (en) * | 2014-10-01 | 2015-01-21 | 姜海艳 | Formula of sheep feed and preparation method of sheep feed |
| US20170333496A1 (en) * | 2016-05-20 | 2017-11-23 | Church & Dwight Co., Inc. | Lactobacillus and bacillus based direct fed microbial treatment for poultry and method of use |
| CN105920619A (en) * | 2016-05-25 | 2016-09-07 | 天津市畜牧兽医研究所 | Method for building weaned-piglet inflammatory response model and application |
| CN106337080A (en) * | 2016-11-29 | 2017-01-18 | 河南科技大学 | Swine escherichia coli pathogenicity test method |
| CN108018228A (en) * | 2017-09-28 | 2018-05-11 | 江苏省农业科学院 | One plant of Escherichia coli O 157:H7 low virulent strains and its application |
| CN108060098A (en) * | 2017-12-13 | 2018-05-22 | 山东大学 | A kind of fermentation culture method of Escherichia coli and its application in feed addictive |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190419 |
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