CN109644790A - A method of improving activities of antioxidant enzymes in tomato seedlings - Google Patents
A method of improving activities of antioxidant enzymes in tomato seedlings Download PDFInfo
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- CN109644790A CN109644790A CN201811607866.3A CN201811607866A CN109644790A CN 109644790 A CN109644790 A CN 109644790A CN 201811607866 A CN201811607866 A CN 201811607866A CN 109644790 A CN109644790 A CN 109644790A
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- tomato
- antioxidant enzymes
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- mycorhiza
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
Abstract
The present invention provides a kind of method for improving activities of antioxidant enzymes in tomato seedlings, and it is respectively 10-35gkg that this method, which is included in application tomato mycorhiza and cyanidin extract, the concentration of the two in tomato seedling culture soil,‑1And 5-10gkg‑1；Wherein, anthocyanidin, which extracts, uses citric acid and alcohol mixed solution, also contains salethyl and ergosterol in the extractant, and be aided with ultraviolet lighting；Wherein, also contain cadmium metal in tomato seedling culture soil, i.e., 15-25mgkg is added in wind desiceted soil‑1CdCl2Solution simultaneously mixes, and balance carried out tomato seedling transplanting after 2-3 weeks.The method that the present invention cultivates tomato, not only increases activities of antioxidant enzymes in tomato body；The method for making tomato growth from heavy metal pollution is additionally provided, the edible safety of tamato fruit is improved；And tomato antimutagenic activity is improved, tomato variety is protected.
Description
Technical field
The invention belongs to field of agricultural cultivation, and in particular to a method of improve activities of antioxidant enzymes in tomato seedlings.
Background technique
Tomato (Tomato) alias tomato, tomato, ancient name persimmon in June, report of good news ternary.In Peru and Mexico, initially
Referred to as " wolf peach ".Fruits nutrition is abundant, has flavour.It can eat raw, cook, being processed into catsup, juice or whole fruit tank
Hiding.Tomato is that one of fruit and vegetable the most universal are cultivated in the whole world.The U.S., the Soviet Union, Italy and China are major producing country.?
There are large area greenhouse, vinyl house and other protecting field facility cultivations in country, China and the Japan in Europe, the United States continent.
Cadmium (Cd) is listed in the first heavy metal pollution of soil object, is widely used in electroplating industry, chemical industry, electronics industry and core
The fields such as industry, the discharge of " three wastes " and sludge dirt filling, municipal refuse etc. aggravate Cd Pollution in Soil increasingly.China
Cd pollution is ploughed up to 11,330,000 hm2, suburb vegetable soil, vegetables Cd pollution the problem of exist in China big and medium-sized cities.Crop
The Cd that root system absorbs easily is migrated to edible part, is then entered enrichment in human body by food chain, is endangered human health.
Anthocyanidin is a kind of water colo(u)r being widely present in plant, belongs to flavones polyphenol compound.It contains virtue
Ring structure and active hydroxyl can terminate free chain reaction, remove the oxygen radical for leading to a variety of diseases, play anti-oxidant
Effect.
The Chinese invention patent of existing 107873419 A of Publication No. CN, discloses a kind of tomato planting method, passes through
It carries out imposing cinnamylaldehyde derivatives Modified Urea at tomato initial stage in florescence, applying cinnamylaldehyde derivatives active constituent can be effective
Promotion potassium element participated in plant adjust Premeabilisation of cells gesture, maintain cell electroneutral, can effectively improve tomato plant
The strain synthesis of protein, the operating and resistance of photosynthate, especially can effectively improve the leaf in tomato plant blade
Chlorophyll contents improve activities of antioxidant enzymes；The Chinese invention patent of 104718840 A of Publication No. CN, discloses a kind of promotion
Method this method of plant seed germination is handled vegetable seeds with epiphysin before being included in sprouting under Cd stress, using taking off
Melanocyte is pre-processed, and the expression of activities of antioxidant enzymes can be induced, and removes a large amount of free radicals generated due to Cd stress, is alleviated
Oxidative stress reduces oxidative damage；And the present invention is while improving activities of antioxidant enzymes in tomato seedlings, additionally providing makes kind
Eggplant grows the method from heavy metal pollution, and improves tomato antimutagenic activity, the method protected to tomato variety.
Summary of the invention
The purpose of the present invention is to provide a kind of methods for improving activities of antioxidant enzymes in tomato seedlings, grow in tomato sprout
Stage improves activities of antioxidant enzymes by regulation nitrogen nutrition proportion and using the artificial red compound light stimulus of indigo plant；In tomato seedling
Growth phase is repaired using Cd stress and tomato mycorhiza and cyanidin extract joint, is higher than Antioxidant Enzyme Systems activity
Normal growth value promotes tomato seedling survival rate and growth rate.
The object of the invention is also to provide making tomato growth from the method for heavy metal pollution, the food of tamato fruit is improved
Use safety.
The present invention also aims to improve tomato antimutagenic activity, tomato variety is protected.
The technical solution that the present invention is taken to achieve the above object are as follows:
A method of it improving activities of antioxidant enzymes in tomato seedlings and tomato cultivating process is managed as follows:
Sprout culture: being respectively 50 × 30 × 20cm by tomato sprout immigration length, width and height when tomato seeds grow a leaf
Water planting slot in, be placed in middle low temperature greenhouse and cultivated；
Seedling culture: it moves into characteristic potting when tomato sprout is grown to three leaves wholeheartedly, is cultivated in greenhouse.
Preferably, sprout cultivation temperature is 10-15 DEG C.
Preferably, during sprout culture forms with nutrient solution, holding total nitrogen concentration is 5-15mmol/L, and nitrate nitrogen: ammonium
The ratio of state nitrogen is 5-10:1；Nitrogen is the mineral nutrient element that plant has the call, and with plant strain growth and development most
For close nutrient, meanwhile, nitrogen is the group of plant vivo protein, nucleic acid, enzyme, chlorophyll and many endogenous hormones
At part and the most important structural material of plant, key substance-enzyme of regulation plant biochemical reaction and its auxiliary is participated in
The synthesis of base.Nitrate nitrogen and ammonium nitrogen are 2 kinds of host inorganic nitrogen that crop can be absorbed；And tomato is happiness nitre crop, with nitre
State nitrogen be in leading nitrogen nutrition environment can growth and development it is good, and high concentration ammonium nitrogen can growth and development to tomato or
Person's yield, quality have adverse effect on.
Preferably, using the artificial red compound light stimulus of indigo plant, light intensity is 150-300 μm of olm during sprout culture-2·
s-1, and light application time is no more than 5 hours daily；Feux rouges and blue light are two kinds of spectrum necessary to plant growth, are that photosynthetic pigments are inhaled
The dominant spectral of receipts；Feux rouges and blue light are about 90% by plant leaf blade absorptivity, and red indigo plant complex light can be superimposed monochromatic with advantage
Feux rouges and blue light advantage, and respective deficiency can be made up；Tomato can not only be promoted using the red blue complex light of proper proportion
The development of photosynthetic organs, promotes photosynthesis, also advantageously improves activities of antioxidant enzymes: SOD increased activity, CAT and POD
Active then complementary trend is presented, CAT activity is higher under low light intensity, and POD activity is lower, and activity is opposite both under high light intensity；SOD,
Whole reinforcement is presented in CAT and POD activity, is better than common light conditions；And with the increase of intensity of illumination, MDA contains in tomato body
Amount slowly decline, not will cause Lipid peroxidation metabolism damage.
Preferably, seedling cultivation temperature is 15-25 DEG C；
Preferably, characteristic potting is the soil of mycorhiza containing tomato and cyanidin extract；The two concentration is respectively 10-
35g·kg-1And 5-10gkg-1。
Preferably, the acquisition methods of tomato mycorhiza are as follows: the tomato place of production fertile soil 100-200g of collection room outgrowth, in
It ferments in the test tube equipped with common PDA culture medium of sterilizing, sufficiently oscillation, which mixes, is placed on 25-30 DEG C of constant-temperature table concussion
36-48h is cultivated, is repeated 2-3 times；The culture solution 2000-3000r/min fermented is centrifuged 3-5min, takes supernatant 6000-
10000r/min is centrifuged 5-8min, takes 0.1-5 μm of filtering with microporous membrane degerming of supernatant, 2-4 DEG C saves backup；Tunning
The biggish impurity of particle first is removed through low-speed centrifugal, then high speed centrifugation obtains tomato mycorhiza extract, contains in the extract more
The beneficial microbe of kind source area tomato growth, such as photosynthetic bacteria, nitrogen-fixing bacteria, bacillus, streptomycete, these growths are beneficial
Bacterium can help Tomato Root System to create advantageous microbiologic population, protect root system infecting from harmful bacteria, virus etc.；Meanwhile
Be conducive to improve Tomato Root System growth adaptation coefficient, accelerate absorption and transport of the root system to nutriment, promote growth.
Preferably, cyanidin extract is selected from one kind of the veterinary antibiotics, cereal, floral extract that are rich in anthocyanidin
Or it is several；Extracting method are as follows: will be enriched in the raw material of anthocyanidin, drying to constant weight at 50-60 DEG C or vacuum freeze drying 24-
It is spare to cross 50-60 mesh then with pulverizer polishing powdering by 32h；Powder raw material is weighed, citric acid and proportion of ethanol is added
For in the mixed solution of 1-1.5:1,55-68 DEG C of extraction 2-4h filters, obtains cyanidin extract.
Preferably, the mass fraction of citric acid is 0.5-0.8%, the volume fraction of ethyl alcohol is 45-65%；Raw material with mention
The solid-liquid ratio for taking agent is 1:15-18g/mL.
Preferably, adding the bigcatkin willow that mass fraction is 0.02-0.05% in extractant in anthocyanidin leaching process
The ergosterol of acetoacetic ester and 0.0001-0.0002%, and it is aided with ultraviolet lighting, light intensity is 30-80 μm of olm-2·s-1, light
According to time 1-2h；The special presence of salethyl and ergosterol, can result in cyanidin extract and is denaturalized, enhance it
While antioxidant activity, the free radical generated to ultraviolet radioactive also has certain scavenging effect；Importantly, after mutation
Cyanidin extract has stronger Scavenging activity to the intracorporal free radical of tomato, and is able to suppress free radical or other stress are made
At tomato dna mutation, tomato variety is protected.
Preferably, characteristic potting is the soil of the cadmium pollution containing simulation；Specific practice are as follows: 3-6kg is crossed into 1-10mm sieve
Wind desiceted soil is packed into plastic tub (diameter × height=30cm × 18cm), and 15-25mgkg is added-1CdCl2Solution simultaneously mixes, and puts down
Weighing apparatus carried out tomato seedling transplanting after 2-3 weeks；Tomato growth is caused to coerce using simulation heavy metal pollution, tomato can be excited
Dynamic change occurs for intracorporal Antioxidant Enzyme Systems activity makes tomato from heavy metal pollution in conjunction with combined remediation method.
Under heavy metal stress, tomato mycorhiza and cyanidin extract carry out joint reparation, can be formed with metal-chelating
Compound reduces harm of the cadmium metal to tomato root and has blocked cadmium to tomato so that cadmium be inhibited to be transported up from tomato root
The pollution of fruit；Simultaneously as adverse environment caused by Cd stress, has caused the active dynamic of Antioxidant Enzyme Systems in tomato body
Variation, when cadmium pollution causes to generate a large amount of active oxygen radicals in tomato body, on the one hand, tomato mycorhiza and cyanidin extract contain
Some antioxidant can remove part free radical, on the other hand, Antioxidant Enzyme Systems SOD, CAT, POD activity in tomato body
Overall activity, which is presented, to be increased, and not only to injure during tomato growth from oxidative stress caused by heavy metal, more be improved
The activity of Antioxidant Enzyme Systems in tomato body, makes it higher than normal level, promotes the dominant growth of tomato seedling instead.
The invention has the benefit that
1) the present invention is directed to improve activities of antioxidant enzymes in tomato seedlings: in tomato sprout growth phase, by regulating and controlling nitrogen
Nutrition-allocated proportion and the artificial red compound light stimulus of indigo plant of use improve activities of antioxidant enzymes；In the Growth of Tomato Seedling stage, coerced using cadmium
Urgent and tomato mycorhiza and cyanidin extract joint are repaired, and Antioxidant Enzyme Systems activity is made to be higher than normal growth value, promotion kind
Eggplant seedling percent and growth rate；
2) present invention is also directed to the method for making tomato growth from heavy metal pollution, improves the edible peace of tamato fruit
Quan Xing；And tomato antimutagenic activity is improved, tomato variety is protected；
3) under heavy metal stress, tomato mycorhiza and cyanidin extract carry out joint reparation, can be with metal-chelating shape
At compound, reduce harm of the cadmium metal to tomato root, so that cadmium be inhibited to be transported up from tomato root, blocked cadmium to kind
The pollution of solanberry reality；Simultaneously as adverse environment caused by Cd stress, it is active dynamic to have caused Antioxidant Enzyme Systems in tomato body
State variation, when cadmium pollution causes to generate a large amount of active oxygen radicals in tomato body, on the one hand, tomato mycorhiza and cyanidin extract
The antioxidant contained can remove part free radical, and on the other hand, Antioxidant Enzyme Systems SOD, CAT, POD are living in tomato body
Property overall activity present and increase, not only to injure from oxidative stress caused by heavy metal during tomato growth, more improve
The activity of Antioxidant Enzyme Systems, makes it higher than normal level, promotes the dominant growth of tomato seedling instead in tomato body；
4) in anthocyanidin extraction process, it is aided with ultraviolet lighting using salethyl and ergosterol, mentions anthocyanidin
Object is taken to be denaturalized, while enhancing its antioxidant activity, the free radical generated to ultraviolet radioactive also has certain scavenging effect；
Importantly, the cyanidin extract after mutation has stronger Scavenging activity to the intracorporal free radical of tomato, and can press down
Tomato dna caused by free radical processed or other stress is mutated, and is protected to tomato variety.
Present invention employs above-mentioned technical proposals to provide model essay, compensates for the deficiencies in the prior art, design is reasonable, operation side
Just.
Specific embodiment
Present invention is further described in detail with reference to embodiments:
Embodiment 1:
A method of it improving activities of antioxidant enzymes in tomato seedlings and tomato cultivating process is managed as follows:
(1) sprout culture: when tomato seeds grow a leaf, by tomato sprout immigration length, width and height be respectively 50 × 30 ×
In the water planting slot of 20cm, it is placed in middle low temperature greenhouse and is cultivated for 10 DEG C；
During sprout culture is formed with nutrient solution, holding total nitrogen concentration is 5mmol/L, and nitrate nitrogen: the ratio of ammonium nitrogen is
5:1；K, Ca, P, Mg concentration are respectively 4,3,2,1mmol/L, and B, Mn, Zn, Cu, Mo concentration are respectively 3,2.5,1.5,0.3,
0.5 μm of ol/L is added dicyandiamide (5 μm of ol/L) and is used as nitrification inhibitor with EDTA-Fe (35 μm of ol/L) for the source Fe；Every 2d changes 1
Secondary nutrient solution, nutrient solution pH are adjusted to 6.0 with 0.1mmol/LNaOH or HCl daily, and nutrient solution concentration is stepped up from 0.5 to complete
Nutrition；Nitrogen is the mineral nutrient element that plant has the call, and the nutrition member the closest with plant strain growth and development
Element, meanwhile, nitrogen is the component part of plant vivo protein, nucleic acid, enzyme, chlorophyll and many endogenous hormones, and is planted
The most important structural material of object participates in the synthesis of the key substance-enzyme and its prothetic group of regulation plant biochemical reaction.Nitre state
Nitrogen and ammonium nitrogen are 2 kinds of host inorganic nitrogen that crop can be absorbed；And tomato is happiness nitre crop, is being leading nitrogen with nitrate nitrogen
In nutrient environment can growth and development it is good, and high concentration ammonium nitrogen can growth and development to tomato or yield, quality produce
Raw detrimental effect；
During sprout culture, using the artificial red compound light stimulus of indigo plant, light intensity is 150 μm of olm-2·s-1, and daily illumination
Time is no more than 5 hours；Feux rouges and blue light are two kinds of spectrum necessary to plant growth, are the dominant spectrals that photosynthetic pigments absorb；
Feux rouges and blue light are about 90% by plant leaf blade absorptivity, and red indigo plant complex light monochromatic feux rouges can be superimposed with advantage and blue light is excellent
Point, and respective deficiency can be made up；It can not only promote the hair of tomato photosynthetic organs using the red blue complex light of proper proportion
Educate, promote photosynthesis, also advantageously improve activities of antioxidant enzymes: SOD increased activity, CAT and POD activity are then presented mutually
Benefit trend, CAT activity is higher under low light intensity, and POD activity is lower, and activity is opposite both under high light intensity；SOD, CAT and POD activity
Whole reinforcement is presented, is better than common light conditions；And with the increase of intensity of illumination, MDA content slowly declines in tomato body, no
It will cause Lipid peroxidation metabolism damage；
(2) seedling is cultivated: moving into characteristic potting when tomato sprout is grown to three leaves wholeheartedly, 15 DEG C of cultures in greenhouse；
Wherein, characteristic potting is the soil of mycorhiza containing tomato and cyanidin extract, and the two concentration is respectively 10gkg-1
And 5gkg-1；
Wherein, the acquisition methods of tomato mycorhiza are as follows: the tomato place of production fertile soil 100g of collection room outgrowth, in the dress of sterilizing
It ferments in the test tube for having common PDA culture medium, sufficiently oscillation mixes and is placed on 25 DEG C of constant-temperature table shake culture 36h, weight
It is 2 times multiple；The culture solution 2000r/min fermented is centrifuged 5min, takes supernatant 6000r/min to be centrifuged 5min, takes supernatant
With 0.1 μm of filtering with microporous membrane degerming, 2 DEG C are saved backup；Tunning first removes the biggish impurity of particle through low-speed centrifugal, so
High speed centrifugation obtains tomato mycorhiza extract afterwards, and the beneficial microbe there are many source area tomato growth, such as light are contained in the extract
Bacterium, nitrogen-fixing bacteria, bacillus, streptomycete etc. are closed, these growth beneficial bacteriums can help Tomato Root System to create advantageous micro- life
Object group protects root system infecting from harmful bacteria, virus etc.；Meanwhile being conducive to improve Tomato Root System growth adaptation coefficient, add
Fast absorption and transport of the root system to nutriment, promotes growth；
Wherein, cyanidin extract is selected from one kind or several of the veterinary antibiotics, cereal, floral extract that are rich in anthocyanidin
Kind；Extracting method are as follows: will be enriched in anthocyanidin raw material drying to constant weight at 50 DEG C or vacuum freeze drying for 24 hours, then use powder
Broken machine polishing powdering, it is spare to cross 50 meshes；Powder raw material is weighed, citric acid and proportion of ethanol is added as the mixed solution of 1:1
In, 55 DEG C of extraction 2h filter, obtain cyanidin extract；The mass fraction of citric acid is 0.5%, and the volume fraction of ethyl alcohol is
45%；The solid-liquid ratio of raw material and extractant is 1:15g/mL；
Wherein, in anthocyanidin leaching process, in extractant add mass fraction be 0.02% salethyl and
0.0001% ergosterol, and it is aided with ultraviolet lighting, light intensity is 30 μm of olm-2·s-1, light application time 1h；Salethyl
With the special presence of ergosterol, it can result in cyanidin extract and be denaturalized, while enhancing its antioxidant activity, to purple
The free radical that external radiation generates also has certain scavenging effect；Importantly, the cyanidin extract after mutation is to tomato body
Interior free radical has stronger Scavenging activity, and is able to suppress tomato dna caused by free radical or other stress and is mutated, right
Tomato variety is protected；
Wherein, characteristic potting is the soil of the cadmium pollution containing simulation；Specific practice are as follows: be packed into the 3kg wind desiceted soil for crossing 1mm sieve
In plastic tub (diameter × height=30cm × 18cm), 15mgkg is added-1CdCl2Solution simultaneously mixes, and balance carries out after 2 weeks
Tomato seedling transplanting；Tomato growth is caused to coerce using simulation heavy metal pollution, the intracorporal antioxidase of tomato can be excited
Dynamic change occurs for system activity makes tomato from heavy metal pollution in conjunction with combined remediation method.
Under heavy metal stress, tomato mycorhiza and cyanidin extract carry out joint reparation, can be formed with metal-chelating
Compound reduces harm of the cadmium metal to tomato root and has blocked cadmium to tomato so that cadmium be inhibited to be transported up from tomato root
The pollution of fruit；Simultaneously as adverse environment caused by Cd stress, has caused the active dynamic of Antioxidant Enzyme Systems in tomato body
Variation, when cadmium pollution causes to generate a large amount of active oxygen radicals in tomato body, on the one hand, tomato mycorhiza and cyanidin extract contain
Some antioxidant can remove part free radical, on the other hand, Antioxidant Enzyme Systems SOD, CAT, POD activity in tomato body
Overall activity, which is presented, to be increased, and not only to injure during tomato growth from oxidative stress caused by heavy metal, more be improved
The activity of Antioxidant Enzyme Systems in tomato body, makes it higher than normal level, promotes the dominant growth of tomato seedling instead.
Embodiment 2:
A method of it improving activities of antioxidant enzymes in tomato seedlings and tomato cultivating process is managed as follows:
(1) sprout culture: when tomato seeds grow a leaf, by tomato sprout immigration length, width and height be respectively 50 × 30 ×
In the water planting slot of 20cm, it is placed in middle low temperature greenhouse and is cultivated for 12 DEG C；
During sprout culture is formed with nutrient solution, holding total nitrogen concentration is 10mmol/L, and nitrate nitrogen: the ratio of ammonium nitrogen is
8:1；K, Ca, P, Mg concentration are respectively 4,3,2,1mmol/L, and B, Mn, Zn, Cu, Mo concentration are respectively 3,2.5,1.5,0.3,
0.5 μm of ol/L is added dicyandiamide (5 μm of ol/L) and is used as nitrification inhibitor with EDTA-Fe (35 μm of ol/L) for the source Fe；Every 2d changes 1
Secondary nutrient solution, nutrient solution pH are adjusted to 6.2 with 0.1mmol/LNaOH or HCl daily, and nutrient solution concentration is stepped up from 0.5 to complete
Nutrition；Nitrogen is the mineral nutrient element that plant has the call, and the nutrition member the closest with plant strain growth and development
Element, meanwhile, nitrogen is the component part of plant vivo protein, nucleic acid, enzyme, chlorophyll and many endogenous hormones, and is planted
The most important structural material of object participates in the synthesis of the key substance-enzyme and its prothetic group of regulation plant biochemical reaction.Nitre state
Nitrogen and ammonium nitrogen are 2 kinds of host inorganic nitrogen that crop can be absorbed；And tomato is happiness nitre crop, is being leading nitrogen with nitrate nitrogen
In nutrient environment can growth and development it is good, and high concentration ammonium nitrogen can growth and development to tomato or yield, quality produce
Raw detrimental effect；
During sprout culture, using the artificial red compound light stimulus of indigo plant, light intensity is 250 μm of olm-2·s-1, and daily illumination
Time is no more than 5 hours；Feux rouges and blue light are two kinds of spectrum necessary to plant growth, are the dominant spectrals that photosynthetic pigments absorb；
Feux rouges and blue light are about 90% by plant leaf blade absorptivity, and red indigo plant complex light monochromatic feux rouges can be superimposed with advantage and blue light is excellent
Point, and respective deficiency can be made up；It can not only promote the hair of tomato photosynthetic organs using the red blue complex light of proper proportion
Educate, promote photosynthesis, also advantageously improve activities of antioxidant enzymes: SOD increased activity, CAT and POD activity are then presented mutually
Benefit trend, CAT activity is higher under low light intensity, and POD activity is lower, and activity is opposite both under high light intensity；SOD, CAT and POD activity
Whole reinforcement is presented, is better than common light conditions；And with the increase of intensity of illumination, MDA content slowly declines in tomato body, no
It will cause Lipid peroxidation metabolism damage；
(2) seedling is cultivated: moving into characteristic potting when tomato sprout is grown to three leaves wholeheartedly, 22 DEG C of cultures in greenhouse；
Wherein, characteristic potting is the soil of mycorhiza containing tomato and cyanidin extract, and the two concentration is respectively 28gkg-1
And 6gkg-1；
Wherein, the acquisition methods of tomato mycorhiza are as follows: the tomato place of production fertile soil 100g of collection room outgrowth, in the dress of sterilizing
It ferments in the test tube for having common PDA culture medium, sufficiently oscillation mixes and is placed on 28 DEG C of constant-temperature table shake culture 40h, weight
It is 3 times multiple；The culture solution 2500r/min fermented is centrifuged 3min, takes supernatant 8000r/min to be centrifuged 5min, takes supernatant
With 2.5 μm of filtering with microporous membrane degermings, 2 DEG C are saved backup；Tunning first removes the biggish impurity of particle through low-speed centrifugal, so
High speed centrifugation obtains tomato mycorhiza extract afterwards, and the beneficial microbe there are many source area tomato growth, such as light are contained in the extract
Bacterium, nitrogen-fixing bacteria, bacillus, streptomycete etc. are closed, these growth beneficial bacteriums can help Tomato Root System to create advantageous micro- life
Object group protects root system infecting from harmful bacteria, virus etc.；Meanwhile being conducive to improve Tomato Root System growth adaptation coefficient, add
Fast absorption and transport of the root system to nutriment, promotes growth；
Wherein, cyanidin extract is selected from one kind or several of the veterinary antibiotics, cereal, floral extract that are rich in anthocyanidin
Kind；Extracting method are as follows: will be enriched in the raw material of anthocyanidin, drying to constant weight at 55 DEG C or vacuum freeze drying 28h, then uses powder
Broken machine polishing powdering, it is spare to cross 60 meshes；Powder raw material is weighed, it is molten for the mixing of 1.2:1 that citric acid and proportion of ethanol is added
In liquid, 65 DEG C of extraction 3h filter, obtain cyanidin extract；The mass fraction of citric acid is 0.6%, and the volume fraction of ethyl alcohol is
60%；The solid-liquid ratio of raw material and extractant is 1:16g/mL；
Wherein, in anthocyanidin leaching process, in extractant add mass fraction be 0.03% salethyl and
0.0001% ergosterol, and it is aided with ultraviolet lighting, light intensity is 50 μm of olm-2·s-1, light application time 2h；Salethyl
With the special presence of ergosterol, it can result in cyanidin extract and be denaturalized, while enhancing its antioxidant activity, to purple
The free radical that external radiation generates also has certain scavenging effect；Importantly, the cyanidin extract after mutation is to tomato body
Interior free radical has stronger Scavenging activity, and is able to suppress tomato dna caused by free radical or other stress and is mutated, right
Tomato variety is protected；
Wherein, characteristic potting is the soil of the cadmium pollution containing simulation；Specific practice are as follows: be packed into the 5kg wind desiceted soil for crossing 5mm sieve
In plastic tub (diameter × height=30cm × 18cm), 20mgkg is added-1CdCl2Solution simultaneously mixes, and balance carries out after 3 weeks
Tomato seedling transplanting；Tomato growth is caused to coerce using simulation heavy metal pollution, the intracorporal antioxidase of tomato can be excited
Dynamic change occurs for system activity makes tomato from heavy metal pollution in conjunction with combined remediation method.
Comparative example 1:
Contain cadmium metal in characteristic potting soil, but is free of tomato mycorhiza and cyanidin extract；Rest part and implementation
Example 2 is completely the same.
Comparative example 2:
Do not contain salethyl and ergosterol in anthocyanidin Extraction solvent, rest part and embodiment 2 are completely the same.
Comparative example 3:
Cadmium metal, tomato mycorhiza and cyanidin extract are free of in tomato seedling culture soil, are general planting soil；Its
Remaining part point and embodiment 2 are completely the same.
Embodiment 3:
Active (active unit: U/ (g is carried out to the tomato (root and leaf) planted under different condition of culture of the invention
min)-1) detection, the results are shown in Table 1；
Tomato (root and leaf) Activity determination under the different condition of culture of table 1
As shown in Table 1, the present invention adds cadmium metal and tomato mycorhiza in tomato seedling culture soil and anthocyanidin mentions
Object is taken, is conducive to improve tomato antioxidant enzymatic activity；The difference is that: when only containing heavy metal in soil, can excite
Activities of antioxidant enzymes increases in tomato body, but tamato fruit does not meet planting requirement vulnerable to heavy metal pollution；And it is coerced in cadmium
Under compeling, tomato mycorhiza is used in combination and cyanidin extract is repaired, tomato antioxidant enzymatic activity can not only be improved, it is also right
Cadmium metal has the function of certain delay and inhibits to propagate, and tamato fruit is made by cadmium pollution, it is edible not can satisfy safety
Standard (in conjunction with table 2).
Each position Cd of 2 tomato of table accumulates content
The prior art of routine techniques dawn known to those skilled in the art in above-described embodiment, therefore herein no longer in detail
It repeats.
The above embodiments are only used to illustrate the present invention, and not limitation of the present invention, the ordinary skill people of this field
Member can also make a variety of changes and modification without departing from the spirit and scope of the present invention.Therefore, all equivalent
Technical solution also belong to scope of the invention, scope of patent protection of the invention should be defined by the claims.
Claims (10)
1. a kind of method for improving activities of antioxidant enzymes in tomato seedlings, it is characterised in that: this method includes carrying out to tomato seedling
Cd stress；The concentration of cadmium ions is 15-25mgkg-1。
2. a kind of method for improving activities of antioxidant enzymes in tomato seedlings according to claim 1, it is characterised in that: this method
It further include that tomato mycorhiza and cyanidin extract joint reparation are carried out to tomato seedling；The tomato mycorhiza and cyanidin extract
Concentration be respectively 10-35gkg-1And 5-10gkg-1。
3. a kind of method for improving activities of antioxidant enzymes in tomato seedlings according to claim 2, it is characterised in that: the flower
Green extract is selected from the one or more of the veterinary antibiotics, cereal, floral extract that are rich in anthocyanidin；Extracting method are as follows: will
Drying to constant weight at 50-60 DEG C or vacuum freeze drying 24-32h for raw material rich in anthocyanidin, is then polished into pulverizer
It is spare to cross 50-60 mesh for powdery；It weighs powder raw material, citric acid is added and proportion of ethanol is in the mixed solution of 1-1.5:1,
55-68 DEG C of extraction 2-4h filters, obtains cyanidin extract.
4. a kind of method for improving activities of antioxidant enzymes in tomato seedlings according to claim 3, it is characterised in that: the flower
In green element leaching process, the salethyl and 0.0001-0.0002% that mass fraction is 0.02-0.05% are added in extractant
Ergosterol, and be aided with ultraviolet lighting, light intensity is 30-80 μm of olm-2·s-1, light application time 1-2h.
5. a kind of method for improving activities of antioxidant enzymes in tomato seedlings according to claim 1, it is characterised in that: described kind
Eggplant cultivating process, is managed as follows:
Sprout culture: being respectively the water of 50 × 30 × 20cm by tomato sprout immigration length, width and height when tomato seeds grow a leaf
It trains in slot, is placed in middle low temperature greenhouse and is cultivated；
Seedling culture: it moves into characteristic potting when tomato sprout is grown to three leaves wholeheartedly, is cultivated in greenhouse.
6. a kind of method for improving activities of antioxidant enzymes in tomato seedlings according to claim 5, it is characterised in that: the bud
Seedling cultivation temperature is 10-15 DEG C；The seedling cultivation temperature is 15-25 DEG C.
7. a kind of method for improving activities of antioxidant enzymes in tomato seedlings according to claim 5, it is characterised in that: the bud
During seedling culture is formed with nutrient solution, holding total nitrogen concentration is 5-15mmol/L, and nitrate nitrogen: the ratio of ammonium nitrogen is 5-10:1.
8. a kind of method for improving activities of antioxidant enzymes in tomato seedlings according to claim 5, it is characterised in that: the bud
During seedling culture, using the artificial red compound light stimulus of indigo plant, light intensity is 150-300 μm of olm-2·s-1, and daily light application time is not
More than 5 hours.
9. a kind of method for improving activities of antioxidant enzymes in tomato seedlings according to claim 5, it is characterised in that: the spy
Color potting is the soil of mycorhiza containing tomato and cyanidin extract；The concentration of the tomato mycorhiza and cyanidin extract is respectively
10-35g·kg-1And 5-10gkg-1。
10. a kind of method for improving activities of antioxidant enzymes in tomato seedlings according to claim 5, it is characterised in that: described
Characteristic potting is the soil of the cadmium pollution containing simulation；Specific practice are as follows: the 3-6kg wind desiceted soil for crossing 1-10mm sieve is packed into plastic tub
It is interior, 15-25mgkg is added-1CdCl2Solution simultaneously mixes, and balance carried out tomato seedling transplanting after 2-3 weeks.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115568384A (en) * | 2022-10-20 | 2023-01-06 | 江南大学 | Application of cerium oxide nano material in enhancing postharvest preservation on the basis of promoting tomato yield and tomato quality |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101828510A (en) * | 2010-05-18 | 2010-09-15 | 新昌县来益生态农业发展有限公司 | Water culture method of tomato with high nutrient content |
| CN105537264A (en) * | 2016-01-19 | 2016-05-04 | 济南益邦生物科技有限公司 | Bioremediation agent for treating compound contaminated soil and application thereof |
| CN107041245A (en) * | 2017-05-22 | 2017-08-15 | 湖南农业大学 | It is a kind of while improving the method that crop nitrogen utilization ratio and Cadmium resistance coerce ability |
| CN108605706A (en) * | 2018-03-20 | 2018-10-02 | 中山大学 | A method of promoting tomato tolerant ability to cadmium |
| CN108967788A (en) * | 2018-08-15 | 2018-12-11 | 谭建新 | A kind of apple ferment drink rich in fruit anthocyanidin and preparation method thereof and device |
-
2018
- 2018-12-27 CN CN201811607866.3A patent/CN109644790A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101828510A (en) * | 2010-05-18 | 2010-09-15 | 新昌县来益生态农业发展有限公司 | Water culture method of tomato with high nutrient content |
| CN105537264A (en) * | 2016-01-19 | 2016-05-04 | 济南益邦生物科技有限公司 | Bioremediation agent for treating compound contaminated soil and application thereof |
| CN107041245A (en) * | 2017-05-22 | 2017-08-15 | 湖南农业大学 | It is a kind of while improving the method that crop nitrogen utilization ratio and Cadmium resistance coerce ability |
| CN108605706A (en) * | 2018-03-20 | 2018-10-02 | 中山大学 | A method of promoting tomato tolerant ability to cadmium |
| CN108967788A (en) * | 2018-08-15 | 2018-12-11 | 谭建新 | A kind of apple ferment drink rich in fruit anthocyanidin and preparation method thereof and device |
Non-Patent Citations (2)
| Title |
|---|
| 丁海东等: "镉和锌对番茄幼苗抗氧化酶活性的影响", 《仲恺农业技术学院学报》 * |
| 史江莉: "不同红/蓝LED组合光源对石榴幼树光合特性和生理指标的影响", 《第二届中国石榴博览会暨第七届全国石榴生产与科研研讨会论文集》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115568384A (en) * | 2022-10-20 | 2023-01-06 | 江南大学 | Application of cerium oxide nano material in enhancing postharvest preservation on the basis of promoting tomato yield and tomato quality |
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Application publication date: 20190419 |