CN109628282B - Microbial cultivation instrument - Google Patents
Microbial cultivation instrument Download PDFInfo
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- CN109628282B CN109628282B CN201910131977.XA CN201910131977A CN109628282B CN 109628282 B CN109628282 B CN 109628282B CN 201910131977 A CN201910131977 A CN 201910131977A CN 109628282 B CN109628282 B CN 109628282B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biotechnology (AREA)
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- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
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- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a microorganism culture instrument, which comprises a first box body, a constant temperature water bath box, an incubator, a handle, an ocular, a lens cone, an objective and a control panel, a constant temperature water bath box is fixed on the bottom plate of the first box body, an incubator is arranged on the upper side of the constant temperature water bath box, an eyepiece is arranged in the middle of the outer side of the top plate of the first box body, a handle is arranged on the outer side of the top plate of the first box body on one side of the eyepiece, a control plate is arranged on the outer side of the top plate of the first box body away from the handle, a lens barrel is arranged below the eyepiece and connected with the objective, the constant temperature water bath box keeps the temperature in the incubator constant, the eyepiece can observe the microorganism condition in the incubator, the handle can control the rotation of the lens cone, meanwhile, the objective lens can rotate, so that the experimenter can observe the conditions in the incubator without dead angles conveniently, and the experimenter can monitor the progress of culture in real time conveniently.
Description
Technical Field
The invention belongs to the field of microbial culture, and particularly relates to a microbial culture instrument.
Background
Bioengineering is an emerging comprehensive application subject beginning to rise in the early 70 th 20 th century, bioengineering based on system theory, namely the concept of system bioengineering, can be used for large-scale culture of 'engineering bacteria' in the 90 th century, and is a very necessary device in modern bioengineering, but the existing microorganism culture apparatus cannot monitor the condition in the incubator, is often only provided with a small observation window which can only see the approximate condition in the incubator, and probably because factors such as water mist and the like influence the sight, the light in the incubator cannot be adjusted, the observation effect is poor, and the culture dish needs to be observed in detail after the culture is finished, but once mixed bacteria are introduced in many biological experiments, the experimental time is greatly wasted, and different phenomena occur in different stages in the culture process, the current incubator also can't carry out real-time observation to the cultivation stage of difference, if need observe the research to the different stages of cultivateing, needs a large amount of observation samples, and current microbial cultivation instrument often does not set up temperature control device independently moreover, and the control by temperature change effect is poor.
Disclosure of Invention
The present invention has been made to solve the above problems, and an object of the present invention is to provide a microorganism culture apparatus.
The invention realizes the above purpose by the following technical proposal, a microorganism culture instrument comprises a device body, a constant temperature water bath box is fixed on a bottom plate of the device body, an incubator is fixed on a top plate of the constant temperature water bath box, an eyepiece is fixed at the middle part of the top plate of the device body, a handle is arranged on the top plate of the device body at one side of the eyepiece, a control plate is arranged at one side of the top plate of the device body, which is far away from the handle, a lens cone is arranged at the middle part of the top plate of the incubator, an objective lens is arranged at the lower end of the lens cone, an objective lens box is arranged below the lens cone, a first motor is fixed on the bottom plate of the objective lens box, a first transmission shaft is arranged at the upper end of the first motor, a gear is fixed at the upper end of the first transmission shaft, the gear is meshed with an outer gear ring, a third connecting rod is fixed on the gear ring, an objective lens is fixed at one side of the third connecting rod, a slide rail is arranged at the lower side of the third connecting rod, and a first small wheel is arranged in the slide rail, the slide rail downside is provided with the movable box, the movable box downside articulates there is the fourth connecting rod, third connecting rod left side is fixed with first fixed block, first fixed block downside is fixed with the second connecting rod, second connecting rod lower extreme is fixed with the second fixed block, second fixed block right side is provided with the second fixed axle, second fixed axle right-hand member is fixed with the carousel, the carousel right side is provided with the spring tube, the carousel middle part is provided with first fixed axle, first fixed axle right side is fixed with the fifth connecting rod, the fifth connecting rod right side is fixed with the hinged-support, be provided with the light on the hinged-support, be provided with the connection piece on the light, the connection piece is articulated with the sixth connecting rod, sixth connecting rod upper end articulates there is the third fixed axle, third fixed axle middle part articulates there is the fourth connecting rod, third fixed axle left end is fixed in the spring tube.
Preferably, a first connecting rod is arranged on the lower side of the handle, a first gear is fixed at the lower end of the first connecting rod and is meshed with a second gear on the right side, a lens barrel is fixed in the middle of the second gear, a bearing is arranged on the upper side of the middle of the second gear, and a first gasket is arranged on the outer side of the top plate of the incubator, where the lens barrel penetrates through the incubator.
Preferably, the bottom plate of the incubator is provided with a first fixing plate, and the right side of the first fixing plate is provided with a second fixing plate.
Preferably, a spring is arranged in the spring pipe, one end of the spring is fixed on the third fixing shaft, one end of the spring, which is far away from the third fixing shaft, is fixed on the baffle, and the baffle is fixed on the inner wall of the spring pipe.
Preferably, the lower end of the lens barrel is provided with a first baffle, the upper end of the first baffle is fixed with a second gasket, the upper end of the objective lens is provided with a second baffle, and the lower end of the second baffle is provided with a third gasket.
Preferably, a group of parallel reflectors is arranged in the lens barrel, and a group of parallel reflectors is arranged in the objective lens.
Advantageous effects
The device comprises a top plate of a device body, a first gear is driven to rotate by rotating the handle, the first gear drives a second gear to rotate, the second gear drives an objective box to rotate, a control panel is arranged on the side, away from the handle, of the top plate of the device body and controls a first motor and a movable box, the first motor is fixed on the bottom plate of the objective box and enables an objective to rotate in a circular ring on the bottom plate of the objective box, the movable box moves in a sliding rail, a fourth connecting rod hinged to the bottom of the movable box enables a third fixed shaft to rotate in a spring tube, the third fixed shaft enables an illuminating lamp to rotate around a hinged support, the illuminating lamp illuminates an incubator, the eyepiece and the objective both have a certain magnification, so that an experimenter can carefully observe microorganisms through the eyepiece, and the bottom of the incubator is provided with a constant temperature water bath box for preserving heat of the incubator.
Drawings
FIG. 1 is a general block diagram of the present invention;
FIG. 2 is an enlarged view of the invention at A;
FIG. 3 is a cross-sectional view B-B of FIG. 2;
FIG. 4 is an enlarged view at C of FIG. 2;
fig. 5 is an enlarged view of fig. 2 at D.
Reference numerals in the figures
1. A device body; 2. a first case; 3. a constant temperature water bath tank; 4. an incubator; 5. a first fixing plate; 6. a first gear; 7. a first connecting rod; 8. a handle; 9. a second gear; 10. an eyepiece; 11. a bearing; 12. a first gasket; 13. a control panel; 14. a lens barrel; 15. a second fixing plate; 16. a second connecting rod; 17. an objective lens box; 18. an objective lens; 19. a third connecting rod; 20. a slide rail; 21. a gear; 22. a first drive shaft; 23. a first motor; 24. a mobile box; 25. a fourth connecting rod; 26. a first fixed block; 27. a turntable; 28. a first fixed shaft; 29. a second fixed shaft; 30. a second fixed block; 31. a spring tube; 32. a fifth connecting rod; 33. an illuminating lamp; 34. a third fixed shaft; 35. a sixth connecting rod; 37. a first small wheel; 39. a mirror; 40. a second gasket; 41. a third gasket; 42. connecting sheets; 43. a spring; 44. a hinged support; 45. a ring gear; 46. a first baffle plate; 47. a second baffle.
Detailed Description
As shown in figures 1-5, a microorganism culture apparatus comprises a device body 1, a constant temperature water bath 3 is fixed on the bottom plate of the device body 1, an incubator 4 is fixed on the top plate of the constant temperature water bath 3, a first fixing plate 5 is arranged on the bottom plate of the incubator 4, a second fixing plate 15 is arranged on the right side of the first fixing plate 5, an eyepiece 10 is fixed on the middle part of the top plate of the device body 1 on one side of the eyepiece 10, a handle 8 is arranged on the top plate of the device body 1 on one side of the eyepiece 10, a first connecting rod 7 is arranged on the lower side of the handle 8, a first gear 6 is fixed on the lower end of the first connecting rod 7, the first gear 6 is meshed with a right second gear 9, a lens barrel 14 is fixed on the middle part of the second gear 9, a bearing 11 is arranged on the upper side of the middle part of the second gear 9, the lens barrel 14 is arranged on the outer side of the top plate of the incubator 4 through the incubator 4, a first gasket 12 is arranged on the top plate side of the eyepiece 10 far away from the handle 8, a control plate 13 is arranged on one side of the top plate of the device body 1, the middle part of the top plate of the incubator 4 is provided with a lens barrel 14, the lower end of the lens barrel 14 is provided with an objective lens 18, two turning parts in the lens barrel 14 are provided with a group of parallel reflectors 39, two turning parts in the objective lens 18 are provided with a group of parallel reflectors 39, the two groups of parallel reflectors 39 enable light of the objective lens 18 to be transmitted to the ocular lens 10 through the lens barrel 14, the lower end of the lens barrel 14 is provided with a first baffle 46, the upper end of the first baffle 46 is fixed with a second gasket 40, the upper end of the objective lens 18 is provided with a second baffle 47, the lower end of the second baffle 47 is provided with a third gasket 41, the first baffle 46 and the second baffle 47 are arranged in a staggered mode to enable the objective lens 18 to rotate, the second gasket 40 and the third gasket 41 seal the joint, and the objective lens box 17 is arranged below the lens barrel 14.
A first motor 23 is fixed on the bottom plate of the objective box 17, a first transmission shaft 22 is arranged at the upper end of the first motor 23, a gear 21 is fixed at the upper end of the first transmission shaft 22, the gear 21 is meshed with an outer gear ring 45, a third connecting rod 19 is fixed on the gear ring 45, an objective 18 is fixed at one side of the third connecting rod 19, which is far away from the gear ring 45, a slide rail 20 is arranged at the lower side of the third connecting rod 19, a first small wheel 37 is arranged in the slide rail 20, a moving box 24 is arranged at the lower side of the slide rail 20, a motor is arranged in the moving box 24, the motor drives the first small wheel 37 to move in the slide rail 20, a fourth connecting rod 25 is hinged at the lower side of the moving box 24, a first fixed block 26 is fixed at the left side of the third connecting rod 19, a second connecting rod 16 is fixed at the lower side of the first fixed block 26, a second fixed block 30 is fixed at the lower end of the second connecting rod 16, a second fixed shaft 29 is arranged at the right side of the second fixed block 30, a rotary table 27 is fixed at the right end of the second fixed shaft 29, the right side of the rotary table 27 is provided with a spring tube 31, a spring 43 is arranged in the spring tube 31, one end of the spring 43 is fixed on a third fixed shaft 34, one end of the spring 43, which is far away from the third fixed shaft 34, is fixed on a baffle plate, the baffle plate is fixed on the inner wall of the spring tube 31, the middle part of the rotary table 27 is provided with a first fixed shaft 28, the right side of the first fixed shaft 28 is fixed with a fifth connecting rod 32, the right side of the fifth connecting rod 32 is fixed with a hinged support 44, the hinged support 44 is provided with an illuminating lamp 33, the illuminating lamp 33 is provided with a connecting sheet 42, the connecting sheet 42 is hinged with a sixth connecting rod 35, the upper end of the sixth connecting rod 35 is hinged with the third fixed shaft 34, the middle part of the third fixed shaft 34 is hinged with a fourth connecting rod 25, and the left end of the third fixed shaft 34 is fixed in the spring tube 31.
When microorganism culture is needed, the constant temperature water bath box 3 is firstly opened to ensure that the first fixing plate 5 and the second fixing plate 15 on the bottom plate of the incubator 4 keep proper temperature, the microorganism culture dish is placed on the first fixing plate 5 and the second fixing plate 15 after reaching proper temperature, when observation needs to be carried out in the incubator 4 in the culture process, the first gear 6 is rotated by rotating the handle 8, the second gear 9 fixed on the lens cone 14 is rotated by the first gear 6, the objective box 17 fixed on the lower side of the lens cone 14 is rotated for 360 degrees around the lens cone 14 by the second gear 9, the first motor 23 is arranged in the objective box 17, the objective 18 rotates in a circular ring on the bottom plate of the objective box 17, the circular ring on the bottom plate is provided with a connecting piece at the joint of the first fixing plate 5 and the second fixing plate 15, the arrangement of the connecting piece does not influence the observation of the objective 18 on the microorganism culture dish on the first fixing plate 5 and the second fixing plate 15, when light is needed to be illuminated downwards, the movable box 24 is moved through the control panel 13, the movable box 24 moves in the direction far away from the first motor 23 in the slide rail 20, the fourth connecting rod 25 moves downwards, the third fixing shaft 34 pushes the spring 43 in the spring tube 31 to move downwards, the sixth connecting rod 35 arranged on the third fixing shaft 34 enables the illuminating lamp 33 to illuminate downwards, an experimenter can see the clearest microorganism culture condition through the eyepiece 10 by adjusting the angle of the illuminating lamp 33, when illumination is not needed, the spring 43 resets, the handle 8 and the control panel 13 are combined, the microorganism culture condition in the first fixing plate 5 and the second fixing plate 15 can be observed in real time, the illuminating lamp 33 capable of steering can enable the observation result to be clearer, the microorganism is amplified through the eyepiece 10 and the objective 18, the experimenter can know the propagation process of the microorganism, and the experimenter can conveniently observe each stage of culture, meanwhile, whether mixed bacteria appear or not can be observed at the initial stage of the experiment, and once the mixed bacteria are found, the mixed bacteria are immediately inoculated again, so that the waste of experiment time is reduced.
The above description is only exemplary of the present invention, and not intended to limit the present invention, and any modifications, equivalents, improvements, etc. made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (4)
1. A microorganism culture instrument comprises a device body and is characterized in that a constant temperature water bath box is fixed on a bottom plate of the device body, an incubator is fixed on a top plate of the constant temperature water bath box, an eyepiece is fixed at the middle part of the top plate of the device body, a handle is arranged on the top plate of the device body on one side of the eyepiece, a control plate is arranged on one side of the top plate of the device body, away from the handle, of the eyepiece, a lens cone is arranged in the middle of the top plate of the incubator, an objective lens is arranged at the lower end of the lens cone, an objective lens box is arranged below the lens cone, a first motor is fixed on the bottom plate of the objective lens, a first transmission shaft is arranged at the upper end of the first motor, a gear is fixed at the upper end of the first transmission shaft, the gear is meshed with an outer gear ring, a third connecting rod is fixed on the gear ring, an objective lens is fixed on one side, away from the gear ring, a slide rail is arranged on the lower side of the third connecting rod, a first small wheel is arranged in the slide rail, a movable box is arranged on the lower side of the slide rail, a fourth connecting rod is hinged to the lower side of the movable box, a first fixed block is fixed to the left side of the third connecting rod, a second connecting rod is fixed to the lower side of the first fixed block, a second fixed block is fixed to the lower end of the second connecting rod, a second fixed shaft is arranged on the right side of the second fixed block, a rotary disc is fixed to the right end of the second fixed shaft, a spring tube is arranged on the right side of the rotary disc, a first fixed shaft is arranged in the middle of the rotary disc, a fifth connecting rod is fixed to the right side of the first fixed shaft, a hinged support is fixed to the right side of the fifth connecting rod, a lighting lamp is arranged on the hinged support, a connecting sheet is arranged on the lighting lamp and hinged to the sixth connecting rod, a third fixed shaft is hinged to the upper end of the sixth connecting rod, a fourth connecting rod is hinged to the middle of the third fixed shaft, and the left end of the third fixed shaft is fixed in the spring tube; a spring is arranged in the spring pipe, one end of the spring is fixed on the third fixed shaft, one end of the spring, which is far away from the third fixed shaft, is fixed on a baffle plate, and the baffle plate is fixed on the inner wall of the spring pipe; the lower side of the handle is provided with a first connecting rod, the lower end of the first connecting rod is fixedly provided with a first gear, the first gear is meshed with a second gear on the right side, the middle part of the second gear is fixedly provided with a lens barrel, the upper side of the middle part of the second gear is provided with a bearing, and the lens barrel penetrates through the incubator and is positioned outside the top plate of the incubator and provided with a first gasket.
2. The microorganism culture apparatus according to claim 1, wherein a first fixing plate is disposed on the bottom plate of the incubator, and a second fixing plate is disposed on the right side of the first fixing plate.
3. The apparatus according to claim 1, wherein the lower end of the lens barrel is provided with a first baffle, a second spacer is fixed to the upper end of the first baffle, a second baffle is arranged at the upper end of the objective, and a third spacer is arranged at the lower end of the second baffle.
4. The apparatus according to claim 1, wherein a set of parallel mirrors is disposed in the lens barrel, and a set of parallel mirrors is disposed in the objective lens.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910131977.XA CN109628282B (en) | 2019-02-22 | 2019-02-22 | Microbial cultivation instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910131977.XA CN109628282B (en) | 2019-02-22 | 2019-02-22 | Microbial cultivation instrument |
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| Publication Number | Publication Date |
|---|---|
| CN109628282A CN109628282A (en) | 2019-04-16 |
| CN109628282B true CN109628282B (en) | 2022-03-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910131977.XA Active CN109628282B (en) | 2019-02-22 | 2019-02-22 | Microbial cultivation instrument |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111575170A (en) * | 2020-06-18 | 2020-08-25 | 李鑫 | Microorganism detection device capable of performing continuous operation detection |
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| JP2006014627A (en) * | 2004-06-30 | 2006-01-19 | Koito Ind Ltd | Culture apparatus |
| CN207108988U (en) * | 2017-05-05 | 2018-03-16 | 徐州工程学院 | A kind of culture dish for Bioexperiment teaching |
| CN207312425U (en) * | 2017-08-18 | 2018-05-04 | 武汉钢铁有限公司 | Belt automatic centring device |
| CN207962204U (en) * | 2017-06-12 | 2018-10-12 | 石城县长庚生态农业发展有限公司 | A kind of tunable light source lighting apparatus used for aquiculture |
| CN109078310A (en) * | 2018-10-25 | 2018-12-25 | 广东全过程工程咨询有限公司 | A kind of shooting basketball automatic spring basketball stands by rolling accumulation of energy |
| CN208654432U (en) * | 2018-06-10 | 2019-03-26 | 曲靖医学高等专科学校 | A kind of Micro biological Tests device of changeable direction of observation |
-
2019
- 2019-02-22 CN CN201910131977.XA patent/CN109628282B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006014627A (en) * | 2004-06-30 | 2006-01-19 | Koito Ind Ltd | Culture apparatus |
| CN207108988U (en) * | 2017-05-05 | 2018-03-16 | 徐州工程学院 | A kind of culture dish for Bioexperiment teaching |
| CN207962204U (en) * | 2017-06-12 | 2018-10-12 | 石城县长庚生态农业发展有限公司 | A kind of tunable light source lighting apparatus used for aquiculture |
| CN207312425U (en) * | 2017-08-18 | 2018-05-04 | 武汉钢铁有限公司 | Belt automatic centring device |
| CN208654432U (en) * | 2018-06-10 | 2019-03-26 | 曲靖医学高等专科学校 | A kind of Micro biological Tests device of changeable direction of observation |
| CN109078310A (en) * | 2018-10-25 | 2018-12-25 | 广东全过程工程咨询有限公司 | A kind of shooting basketball automatic spring basketball stands by rolling accumulation of energy |
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| CN109628282A (en) | 2019-04-16 |
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