CN109626771A - A method of promote anaerobically digested sludge heavy metal stable - Google Patents
A method of promote anaerobically digested sludge heavy metal stable Download PDFInfo
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- CN109626771A CN109626771A CN201811526137.5A CN201811526137A CN109626771A CN 109626771 A CN109626771 A CN 109626771A CN 201811526137 A CN201811526137 A CN 201811526137A CN 109626771 A CN109626771 A CN 109626771A
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- 239000010802 sludge Substances 0.000 title claims abstract description 85
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 37
- 241000894006 Bacteria Species 0.000 claims abstract description 75
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 42
- 230000029087 digestion Effects 0.000 claims abstract description 38
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 35
- 229910052742 iron Inorganic materials 0.000 claims abstract description 17
- 241000015585 Corynebacterium humireducens Species 0.000 claims abstract description 13
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 8
- 229910052793 cadmium Inorganic materials 0.000 claims description 20
- 229910052804 chromium Inorganic materials 0.000 claims description 20
- 229910052802 copper Inorganic materials 0.000 claims description 20
- 229910052745 lead Inorganic materials 0.000 claims description 20
- 229910052759 nickel Inorganic materials 0.000 claims description 17
- 229910052725 zinc Inorganic materials 0.000 claims description 17
- 230000033116 oxidation-reduction process Effects 0.000 claims description 8
- 206010035664 Pneumonia Diseases 0.000 claims description 3
- 239000003864 humus Substances 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims description 2
- 230000008569 process Effects 0.000 abstract description 13
- 229910052500 inorganic mineral Inorganic materials 0.000 abstract description 6
- 239000011707 mineral Substances 0.000 abstract description 6
- 239000005416 organic matter Substances 0.000 abstract description 3
- 238000011946 reduction process Methods 0.000 abstract description 3
- 238000006243 chemical reaction Methods 0.000 description 25
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
- 239000001963 growth medium Substances 0.000 description 12
- 229910052751 metal Inorganic materials 0.000 description 12
- 239000002184 metal Substances 0.000 description 12
- 238000011065 in-situ storage Methods 0.000 description 10
- 239000010865 sewage Substances 0.000 description 10
- 238000004458 analytical method Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 238000002161 passivation Methods 0.000 description 8
- 238000000855 fermentation Methods 0.000 description 7
- 238000012545 processing Methods 0.000 description 7
- 229910021646 siderite Inorganic materials 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 239000006228 supernatant Substances 0.000 description 7
- 239000001888 Peptone Substances 0.000 description 6
- 108010080698 Peptones Proteins 0.000 description 6
- 238000011534 incubation Methods 0.000 description 6
- 238000001095 inductively coupled plasma mass spectrometry Methods 0.000 description 6
- 229910001629 magnesium chloride Inorganic materials 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 235000019319 peptone Nutrition 0.000 description 6
- 238000007789 sealing Methods 0.000 description 6
- 239000011780 sodium chloride Substances 0.000 description 6
- 229940041514 candida albicans extract Drugs 0.000 description 5
- 238000001914 filtration Methods 0.000 description 5
- 239000002068 microbial inoculum Substances 0.000 description 5
- 239000012138 yeast extract Substances 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 4
- 244000005700 microbiome Species 0.000 description 3
- 241000589519 Comamonas Species 0.000 description 2
- 229910017518 Cu Zn Inorganic materials 0.000 description 2
- 241000588747 Klebsiella pneumoniae Species 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000009418 renovation Methods 0.000 description 2
- 239000013049 sediment Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 241000607142 Salmonella Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000010564 aerobic fermentation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 238000000975 co-precipitation Methods 0.000 description 1
- 238000009264 composting Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000000937 inactivator Effects 0.000 description 1
- 229910001608 iron mineral Inorganic materials 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
- C02F2101/22—Chromium or chromium compounds, e.g. chromates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Treatment Of Sludge (AREA)
Abstract
The invention discloses a kind of stable method of promotion anaerobically digested sludge heavy metal, this method is that iron-reducing bacterium agent will be added in anaerobically digested sludge, in 25~37 DEG C anaerobic digestion 20~60 days；The iron-reducing bacterium agent is selected from least one of Corynebacterium humireducens, iron-reduced tuftedmonas and Friedlander's bacillus.The method of the present invention can make heavy metal form stable mineral, to realize the quick fixation of heavy metal.The mineralising of organic matter is coupled in iron-reducing bacterium iron reduction process, while directly accelerating the process of sludge digestion.
Description
Technical field
The invention belongs to field of environment protection, and in particular to one kind is inoculated with iron-reducing bacterium bacterium during anaerobic sludge digestion
Agent and the method for promoting heavy metal stable.
Background technique
Municipal sludge is the sediment of generation during municipal sewage treatment.More than 4,000 ten thousand tons of sludge of China's annual output, and
Speed with annual 15% increases.Therefore, how scientific and reasonable disposition municipal sludge is environmental problem urgently to be solved.At present
Mud decrement-is harmless-and stabilization treatment method is mainly aerobic fermentation, anaerobic digestion, anhydration and incineration etc..But due to sludge
It is enriched most heavy metal in sewage disposal process, the content of beary metal such as Cu, Pb, Cr, Cd are high, and recycling sludge utilized
Heavy metal cannot achieve effective control in journey, and there are huge environmental risks for the sludge after disposition.
Currently, sludge heavy-metal recovery technique is mainly in-situ passivation technology, i.e. addition passivator changes at sludge composting
The form of heavy metal (Cu, Pb, Cr, Cd) in reason, to realize the in-situ passivation of heavy metal.Chemical inactivators are at high cost, secondary
It is seriously polluted.First choice of the microorganism in situ passivating technique because of the advantages that at low cost, without secondary pollution as heavy metal passivating technique.
Heavy metal microorganism in situ passivating technique is using certain micro-organisms to the absorption of heavy metal, precipitating, redox
The effects of, to reduce the toxicity and migration of heavy metal.Patent CN 103752604A discloses a kind of charcoal and iron reduction
The method of microbial inoculum renovation of heavy metal polluted soil with combined, patent CN 102443400B disclose one kind and can be applied to soil and river
Gush the ferriferous oxide and humus and its reducing bacteria tri compound renovation agent and preparation method thereof of sediment in-situ organic contamination reparation.
Through retrieving, it is not yet found that iron-reducing bacterium is applied to the report of anaerobic sludge digestion heavy metals in process passivation.
Summary of the invention
The present invention provides a kind of method that iron-reducing bacterium promotes anaerobic sludge digestion heavy metals in process stable.
The technical solution used in the present invention is:
Application of the iron-reducing bacterium agent in promotion anaerobically digested sludge heavy metal is stablized, the iron-reducing bacterium agent are selected from humic
Matter restores at least one of bar bacterium, iron-reduced tuftedmonas and Friedlander's bacillus.
Preferably, the heavy metal includes Cu, Zn, Pb, Cd, Cr, Ni.
A method of promote anaerobically digested sludge heavy metal stable, comprising the following steps:
Will in anaerobically digested sludge add iron-reducing bacterium agent, in 25~37 DEG C anaerobic digestion 20~60 days；
The iron-reducing bacterium agent is in Corynebacterium humireducens, iron-reduced tuftedmonas and Friedlander's bacillus
At least one.
Preferably, the deposit number of the Corynebacterium humireducens is CGMCC No.2452；The iron restores feathering list
The deposit number of born of the same parents bacterium is CGMCC No.1968；The deposit number of the Friedlander's bacillus is CGMCC No.2454.
Preferably, the iron-reducing bacterium agent of 0.5%~5%v/v is added in every liter of anaerobically digested sludge.
Preferably, concentration >=6 × 10 of the iron-reducing bacterium viable count in the iron-reducing bacterium agent9CFU/mL。
Preferably, it is added before iron-reducing bacterium agent, anaerobically digested sludge first leads to N2To oxidation-reduction potential -300mV with
Under.
Preferably, revolving speed is 120~220rpm when anaerobic digestion.
Preferably, the heavy metal includes Cu, Zn, Pb, Cd, Cr, Ni.
Preferably, the TCOD of the anaerobically digested sludge be 5000~35000mg/L, pH be 5~7.5, SCOD 500~
10000~40000mg/L of 1500mg/L, TS 10000~40000mg/L, VS 10000~30000mg/L, SS；VSS10000
~30000mg/L, NH420~100mg/L of-N 100~150mg/L, VFAs.
The beneficial effects of the present invention are:
The method of the present invention can make heavy metal form stable mineral, to realize the quick fixation of heavy metal.Iron reduction
The mineralising of organic matter is coupled in bacterium iron reduction process, while directly accelerating the process of sludge digestion.
Detailed description of the invention
The vivianite SEM figure formed after anaerobic digestion after Fig. 1 addition iron-reducing bacterium microbial inoculum；
The siderite SEM figure formed after anaerobic digestion after Fig. 2 addition iron-reducing bacterium microbial inoculum；
The SEM-EDS map of vivianite (vivianite) in Fig. 3 anaerobic digestion process；
The SEM-EDS map of siderite (siderite) in Fig. 4 anaerobic digestion process.
Specific embodiment
The present invention is further illustrated combined with specific embodiments below.
Anaerobically digested sludge is acquired from certain sewage plant secondary settling tank returned sludge.The sludge fetched first uses the screen to filtrate of 40 mesh
It is quiet again to sink for 24 hours, supernatant is removed, the sludge after concentration is then saved in the refrigerator that temperature is 4 DEG C.Excess sludge after concentration
Basic physics and chemistry matter is as follows: pH 6.26, TCOD 32400mg/L, SCOD 708mg/L, TS 33578mg/L, VS 22456mg/L；
SS 32582mg/L；VSS 20695mg/L, NH4- N 125mg/L, VFAs mg/L 26.Heavy Metals content be Cu, Zn,
The concentration of Pb, Cd, Cr, Ni are respectively 548.4,1182.8,98.4,10.5,218.8 and 87.4mg/L.
Embodiment 1
(1) anaerobically digested sludge TCOD 30000mg/L, pH are adjusted to 7.0 or so, leads to N2To oxidation-reduction potential (ORP)
When less than -300mV (usual 30min).Then, in N2Protection under sludge is packed into specification is 500mL, swept volume is
The anaerobic reaction bottle of 300mL, while in anaerobic reaction bottle, every 1L anaerobically digested sludge adds the iron-reducing bacterium agent of 1%v/v
(concentration >=6 × 10 of living bacteria count in iron-reducing bacterium agent9CFU/mL), cover tightly bottle cap and seal up membrana oralis sealing.Not to be inoculated with
The processing of iron-reducing bacterium agent is control.
From certain sewage plant secondary settling tank returned sludge, (this experiment seed sludge uses sewage for above-mentioned anaerobically digested sludge acquisition
Treatment plant's fresh activity sludge), the sludge fetched first uses the screen to filtrate of 40 mesh is quiet again to sink for 24 hours, supernatant is removed, then in temperature
Degree is the sludge after preservation concentration in 4 DEG C of refrigerator.The basic physical and chemical matter of excess sludge is as follows after concentration: pH 6.26, TCOD
32400mg/L, SCOD 708mg/L, TS 33578mg/L, VS 22456mg/L；SS 32582mg/L；VSS 20695mg/L,
NH4- N 125mg/L, VFAs 26mg/L.Heavy Metals content be the concentration of Cu, Zn, Pb, Cd, Cr, Ni be respectively 548.4,
1182.8,98.4,10.5,218.8 and 87.4mg/L.Activated state heavy metal analysis: 1mol/L magnesium chloride, 180rpm, 25 are used
DEG C shaking table extracts 2h, ICP-MS measurement.
Above-mentioned iron-reducing bacterium agent is by Corynebacterium humireducens (Corynebacterium humireducens
CGMCC2452), iron-reduced tuftedmonas (Comamonas ferrireducens CGMCC 1968) and e coil k 1 pneumonia
3 plants of bacterial strain mixed culture of bacterium (Klebsiella pneumoniae CGMCC 1968) are made, and Spawn incubation method is as follows: by this
3 kinds of bacterium are seeded to LB culture medium, 7.0,30 DEG C of 48~72h of aerobic culture of pH；LB culture medium composition are as follows: peptone 10g/L, ferment
Female extract 5g/L, sodium chloride 10g/L.
(2) anaerobic digestion.Anaerobic reaction bottle is placed in shaking table and carries out anaerobic digestion.Shaking speed is set as 150rpm.
Reaction temperature is 35 DEG C, and anaerobic fermentation digests 30d.The experiment uses intermediate temperature anaerobic fermentation digestion process.
(3) Speciation Analysis of Heavy Metals.After the completion of anaerobic digestion, sludge activity state content of beary metal is detected.
The result shows that: 5 kinds of activated state content of beary metal such as Cu, Zn, Pb, Cd, Cr, Ni are down to 29.61 respectively, 85.47,
2.95,0.50,5.25,1.05mg/L, substantially less than control treatment 39.48,127.74,4.72,0.63,6.56,1.57mg/
L (is shown in Table 1).
SEM has found, during anaerobic sludge digestion, under iron-reducing bacterium effect, Fe oxides are significantly changed,
And form two kinds of ferriferous oxide vivianites (Fig. 1) and siderite (Fig. 2).Adhere on SEM-EDS map, on iron mineral big
Heavy metal Cd is measured, and Cd tapers off trend around mineral, illustrates that ferriferous oxide forms vivianite at mine in two secondary pollutants
(vivianite) it can be enriched with surrounding Cd during and be co-precipitated, reached heavy metal in-situ passivation effect (Fig. 3).
And the siderite surface (siderite) and around, be evenly distributed around Cd, illustrate ferriferous oxide in two secondary pollutants at mine shape
Co-precipitation (Fig. 4) cannot occur with Cd around during at siderite.This shows the distribution of Cd and Lan Tie in mineral
There are certain correlations for mine crystal positions, that is, have the position Cd distribution of vivianite mineral significantly to increase, this also indicates that secondary shape
At vivianite facilitate conversion of the activated state Cd to stationary state Cd in cultivating system.It is potentially based on this, in anaerobic digestion process
Middle addition iron-reducing bacterium realizes the in-situ passivation of the heavy metals such as Cu, Pb, Cr, Cd.
1. activated state content of beary metal of table changes (mg/L)
Embodiment 2
(1) anaerobically digested sludge TCOD 20000mg/L, pH are adjusted to 7.0 or so, leads to N2To oxidation-reduction potential (ORP)
When less than -300mV (usual 30min).Then, in N2Protection under sludge is packed into specification is 500mL, swept volume is
The anaerobic reaction bottle of 300mL, while in anaerobic reaction bottle, every 1L anaerobically digested sludge adds the iron-reducing bacterium agent of 2%v/v
(concentration >=6 × 10 of living bacteria count in iron-reducing bacterium agent9CFU/mL), cover tightly bottle cap and seal up membrana oralis sealing.Not to be inoculated with
The processing of iron-reducing bacterium agent is control.
From certain sewage plant secondary settling tank returned sludge, the sludge fetched first uses the sieve of 40 mesh for above-mentioned anaerobically digested sludge acquisition
Filtering is quiet again to sink for 24 hours, removes supernatant, the sludge after concentration is then saved in the refrigerator that temperature is 4 DEG C.It is remaining dirty after concentration
The basic physical and chemical matter of mud is as follows: pH 6.26, TCOD 32400mg/L, SCOD 708mg/L, TS 33578mg/L, VS
22456mg/L；SS 32582mg/L；VSS 20695mg/L, NH4- N 125mg/L, VFAs 26mg/L.Heavy Metals content
Concentration for Cu, Zn, Pb, Cd, Cr, Ni is respectively 548.4,1182.8,98.4,10.5,218.8 and 87.4mg/L.Activated state
Heavy metal analysis: 2h, ICP-MS measurement are extracted using 1mol/L magnesium chloride, 180rpm, 25 DEG C of shaking tables.
Above-mentioned iron-reducing bacterium agent is by Corynebacterium humireducens (Corynebacterium humireducens, CGMCC
No.2452), iron-reduced tuftedmonas (Comamonas ferrireducens, CGMCC No.1968) and kerekou pneumonia primary
3 plants of bacterial strain mixed culture of Salmonella (Klebsiella pneumoniae, CGMCC No.2454) are made, and Spawn incubation method is such as
Under: 3 kinds of bacterium are seeded to LB culture medium, 7.0,30 DEG C of 48~72h of aerobic culture of pH；LB culture medium composition are as follows: peptone
10g/L, yeast extract 5g/L, sodium chloride 10g/L.
(2) anaerobic digestion.Anaerobic reaction bottle is placed in shaking table and carries out anaerobic digestion.Shaking speed is set as 150rpm.
Reaction temperature is 30 DEG C.Anaerobic fermentation 40d.
(3) Speciation Analysis of Heavy Metals.After the completion of anaerobic digestion, sludge activity state content of beary metal is detected.
The result shows that 5 kinds of activated state content of beary metal such as Cu, Zn, Pb, Cd, Cr, Ni reduce respectively with comparing
20.69%, 20.22%, 17.53%, 15.56%, 14.78% and 31.67%.Illustrate to add the iron in anaerobic digestion process
Reducing bacteria can be realized the in-situ passivation (being shown in Table 2) of the heavy metals such as Cu, Zn, Pb, Cr, Cd, Ni.
2. activated state content of beary metal of table changes (mg/L)
Embodiment 3
(1) anaerobically digested sludge TCOD 10000mg/L, pH are adjusted to 7.0 or so, leads to N2To oxidation-reduction potential (ORP)
When less than -300mV (usual 30min).Then, in N2Protection under sludge is packed into specification is 500mL, swept volume is
The anaerobic reaction bottle of 300mL, while in anaerobic reaction bottle, every 1L anaerobically digested sludge adds the iron-reducing bacterium agent of 2%v/v
(concentration >=6 × 10 of living bacteria count in iron-reducing bacterium agent9CFU/mL), cover tightly bottle cap and seal up membrana oralis sealing.Not to be inoculated with
The processing of iron-reducing bacterium agent is control.
From certain sewage plant secondary settling tank returned sludge, the sludge fetched first uses the sieve of 40 mesh for above-mentioned anaerobically digested sludge acquisition
Filtering is quiet again to sink for 24 hours, removes supernatant, the sludge after concentration is then saved in the refrigerator that temperature is 4 DEG C.It is remaining dirty after concentration
The basic physical and chemical matter of mud is as follows: pH 6.26, TCOD 32400mg/L, SCOD 708mg/L, TS 33578mg/L, VS
22456mg/L；SS 32582mg/L；VSS 20695mg/L, NH4- N 125mg/L, VFAs 26mg/L.Heavy Metals content
Concentration for Cu, Zn, Pb, Cd, Cr, Ni is respectively 548.4,1182.8,98.4,10.5,218.8 and 87.4mg/L.Activated state
Heavy metal analysis: 2h, ICP-MS measurement are extracted using 1mol/L magnesium chloride, 180rpm, 25 DEG C of shaking tables.
Above-mentioned iron-reducing bacterium agent is by Corynebacterium humireducens, iron-reduced tuftedmonas and 3 plants of Friedlander's bacillus
Bacterial strain mixed culture is made, and Spawn incubation method is as follows: 3 kinds of bacterium are seeded to LB culture medium, 7.0,30 DEG C of aerobic cultures of pH
48~72h；LB culture medium composition are as follows: peptone 10g/L, yeast extract 5g/L, sodium chloride 10g/L.
(2) anaerobic digestion.Anaerobic reaction bottle is placed in shaking table and carries out anaerobic digestion.Shaking speed is set as 200rpm.
Reaction temperature is 37 DEG C.Anaerobic fermentation 40d.
(3) Speciation Analysis of Heavy Metals.After the completion of anaerobic digestion, sludge activity state content of beary metal is detected.As a result
Show and compare, Cu (II), Cr (VI) content reduce by 11.80% respectively, 23.55%, 27.37%, 20.00%,
16.43% and 26.15% (being shown in Table 3).Illustrate to add in anaerobic digestion process the iron-reducing bacterium can be realized Cu, Zn, Pb,
The in-situ passivation of the heavy metals such as Cr, Cd, Ni.
3. activated state content of beary metal of table changes (mg/L)
| Processing | Cu | Zn | Pb | Cd | Cr | Ni |
| Group | 15.25 | 48.24 | 1.92 | 0.30 | 2.81 | 0.65 |
| Control group | 13.45 | 36.88 | 1.38 | 0.24 | 2.34 | 0.48 |
| Decreasing value (%) | 11.80 | 23.55 | 27.37 | 20.00 | 16.43 | 26.15 |
Embodiment 4
(1) anaerobically digested sludge TCOD 10000mg/L, pH are adjusted to 7.0 or so, leads to N2To oxidation-reduction potential (ORP)
When less than -300mV (usual 30min).Then, in N2Protection under sludge is packed into specification is 500mL, swept volume is
The anaerobic reaction bottle of 300mL, while in anaerobic reaction bottle, every 1L anaerobically digested sludge adds the iron-reducing bacterium agent of 3%v/v
(concentration >=6 × 10 of living bacteria count in iron-reducing bacterium agent9CFU/mL), cover tightly bottle cap and seal up membrana oralis sealing.Not to be inoculated with
The processing of iron-reducing bacterium agent is control.
From certain sewage plant secondary settling tank returned sludge, the sludge fetched first uses the sieve of 40 mesh for above-mentioned anaerobically digested sludge acquisition
Filtering is quiet again to sink for 24 hours, removes supernatant, the sludge after concentration is then saved in the refrigerator that temperature is 4 DEG C.It is remaining dirty after concentration
The basic physical and chemical matter of mud is as follows: pH 6.26, TCOD 32400mg/L, SCOD 708mg/L, TS 33578mg/L, VS
22456mg/L；SS 32582mg/L；VSS 20695mg/L, NH4- N 125mg/L, VFAs 26mg/L.Heavy Metals content
Concentration for Cu, Zn, Pb, Cd, Cr, Ni is respectively 548.4,1182.8,98.4,10.5,218.8 and 87.4mg/L.Activated state
Heavy metal analysis: 2h, ICP-MS measurement are extracted using 1mol/L magnesium chloride, 180rpm, 25 DEG C of shaking tables.
Above-mentioned iron-reducing bacterium agent is by Corynebacterium humireducens, iron-reduced tuftedmonas and 3 plants of Friedlander's bacillus
Bacterial strain mixed culture is made, and Spawn incubation method is as follows: 3 kinds of bacterium are seeded to LB culture medium, 7.0,30 DEG C of aerobic cultures of pH
48~72h；LB culture medium composition are as follows: peptone 10g/L, yeast extract 5g/L, sodium chloride 10g/L.
(2) anaerobic digestion.Anaerobic reaction bottle is placed in shaking table and carries out anaerobic digestion.Shaking speed is set as 220rpm.
Reaction temperature is 30 DEG C.Anaerobic fermentation 30d.
(3) Speciation Analysis of Heavy Metals.After the completion of anaerobic digestion, sludge activity state content of beary metal is detected.
The result shows that with comparing, Cu, Zn, Pb, Cr, Cd, Ni content reduces by 10.82% respectively, 20.14%,
21.35%, 17.36,14.25% and 21.03% (being shown in Table 4).Illustrating to add the iron-reducing bacterium in anaerobic digestion process can
Realize the in-situ passivation of the heavy metals such as Cu, Zn, Pb, Cr, Cd, Ni.
4. activated state content of beary metal of table changes (mg/L)
| Group | Cu | Zn | Pb | Cd | Cr | Ni |
| Control group | 16.25 | 50.24 | 2.21 | 0.32 | 2.94 | 0.71 |
| Iron-reducing bacterium microbial inoculum | 14.49 | 40.12 | 1.74 | 0.26 | 2.52 | 0.56 |
| Decreasing value (%) | 10.82 | 20.14 | 21.35 | 17.36 | 14.25 | 21.03 |
Embodiment 5
(1) anaerobically digested sludge TCOD 10000mg/L, pH are adjusted to 7.0 or so, leads to N2To oxidation-reduction potential (ORP)
When less than -300mV (usual 30min).Then, in N2Protection under sludge is packed into specification is 500mL, swept volume is
The anaerobic reaction bottle of 300mL, while in anaerobic reaction bottle, every 1L anaerobically digested sludge adds the iron-reducing bacterium agent of 5%v/v
(concentration >=6 × 10 of living bacteria count in iron-reducing bacterium agent9CFU/mL), cover tightly bottle cap and seal up membrana oralis sealing.Not to be inoculated with
The processing of iron-reducing bacterium agent is control.
From certain sewage plant secondary settling tank returned sludge, the sludge fetched first uses the sieve of 40 mesh for above-mentioned anaerobically digested sludge acquisition
Filtering is quiet again to sink for 24 hours, removes supernatant, the sludge after concentration is then saved in the refrigerator that temperature is 4 DEG C.It is remaining dirty after concentration
The basic physical and chemical matter of mud is as follows: pH7.5, TCOD 32400mg/L, SCOD 708mg/L, TS 33578mg/L, VS 22456mg/
L；SS 32582mg/L；VSS 20695mg/L, NH4- N 125mg/L, VFAs 26mg/L.Heavy Metals content be Cu, Zn,
The concentration of Pb, Cd, Cr, Ni are respectively 548.4,1182.8,98.4,10.5,218.8 and 87.4mg/L.The inspection of activated state heavy metal
It surveys: extracting 2h, ICP-MS measurement using 1mol/L magnesium chloride, 180rpm, 25 DEG C of shaking tables.
Above-mentioned iron-reducing bacterium agent is made of Corynebacterium humireducens culture, and Spawn incubation method is as follows: the bacterium is inoculated with
To LB culture medium, 7.0,30 DEG C of 48~72h of aerobic culture of pH；LB culture medium composition are as follows: peptone 10g/L, yeast extract
5g/L, sodium chloride 10g/L.
(2) anaerobic digestion.Anaerobic reaction bottle is placed in shaking table and carries out anaerobic digestion.Shaking speed is set as 220rpm.
Reaction temperature is 30 DEG C, anaerobic fermentation 50d.
Embodiment 6
(1) anaerobically digested sludge TCOD 10000mg/L, pH are adjusted to 7.0 or so, leads to N2To oxidation-reduction potential (ORP)
When less than -300mV (usual 30min).Then, in N2Protection under sludge is packed into specification is 500mL, swept volume is
The anaerobic reaction bottle of 300mL, while in anaerobic reaction bottle, every 1L anaerobically digested sludge adds the iron-reducing bacterium agent of 5%v/v
(concentration >=6 × 10 of living bacteria count in iron-reducing bacterium agent9CFU/mL), cover tightly bottle cap and seal up membrana oralis sealing.Not to be inoculated with
The processing of iron-reducing bacterium agent is control.
From certain sewage plant secondary settling tank returned sludge, the sludge fetched first uses the sieve of 40 mesh for above-mentioned anaerobically digested sludge acquisition
Filtering is quiet again to sink for 24 hours, removes supernatant, the sludge after concentration is then saved in the refrigerator that temperature is 4 DEG C.It is remaining dirty after concentration
The basic physical and chemical matter of mud is as follows: pH 5, TCOD 32400mg/L, SCOD 708mg/L, TS 33578mg/L, VS 22456mg/
L；SS 32582mg/L；VSS 20695mg/L, NH4- N 125mg/L, VFAs 26mg/L.Heavy Metals content be Cu, Zn,
The concentration of Pb, Cd, Cr, Ni are respectively 548.4,1182.8,98.4,10.5,218.8 and 87.4mg/L.The inspection of activated state heavy metal
It surveys: extracting 2h, ICP-MS measurement using 1mol/L magnesium chloride, 180rpm, 25 DEG C of shaking tables.
Above-mentioned iron-reducing bacterium agent is made of Corynebacterium humireducens culture, and Spawn incubation method is as follows: the bacterium is inoculated with
To LB culture medium, 7.0,30 DEG C of 48~72h of aerobic culture of pH；LB culture medium composition are as follows: peptone 10g/L, yeast extract
5g/L, sodium chloride 10g/L.
(2) anaerobic digestion.Anaerobic reaction bottle is placed in shaking table and carries out anaerobic digestion.Shaking speed is set as 220rpm.
Reaction temperature is 37 DEG C, anaerobic fermentation 20d.
In conclusion the additional iron-reducing bacterium microbial inoculum of the present invention can make heavy metal form stable mineral, to realize weight
The quick fixation of metal.The mineralising of organic matter is coupled in iron-reducing bacterium iron reduction process, directly accelerates the process of sludge digestion.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (10)
1. application of the iron-reducing bacterium agent in promotion anaerobically digested sludge heavy metal is stablized, the iron-reducing bacterium agent are selected from humus
Restore at least one of bar bacterium, iron-reduced tuftedmonas and Friedlander's bacillus.
2. application according to claim 1, which is characterized in that the heavy metal includes Cu, Zn, Pb, Cd, Cr, Ni.
3. a kind of method for promoting anaerobically digested sludge heavy metal stable, which comprises the following steps:
Will in anaerobically digested sludge add iron-reducing bacterium agent, in 25~37 DEG C anaerobic digestion 20~60 days；
The iron-reducing bacterium agent in Corynebacterium humireducens, iron-reduced tuftedmonas and Friedlander's bacillus extremely
Few one kind.
4. according to the method described in claim 3, it is characterized in that, the deposit number of the Corynebacterium humireducens is
CGMCC No.2452；The deposit number of the iron-reduced tuftedmonas is CGMCC No.1968；The e coil k 1 pneumonia
The deposit number of bacterium is CGMCC No.2454.
5. the method according to claim 3, which is characterized in that add 0.5%~5% in every liter of anaerobically digested sludge
The iron-reducing bacterium agent of v/v.
6. the method according to claim 3 or 4, which is characterized in that the iron-reducing bacterium viable count in the iron-reducing bacterium agent
Concentration >=6 × 109CFU/mL。
7. the method according to claim 3, which is characterized in that be added before iron-reducing bacterium agent, anaerobically digested sludge
First lead to N2To oxidation-reduction potential below -300mV.
8. according to the method described in claim 3, it is characterized in that, revolving speed is 120~220rpm when anaerobic digestion.
9. according to the method described in claim 3, it is characterized in that, the heavy metal includes Cu, Zn, Pb, Cd, Cr, Ni.
10. according to the method described in claim 3, it is characterized in that, the TCOD of the anaerobically digested sludge be 5000~
35000mg/L, pH be 5~7.5, SCOD, 500~1500mg/L, TS 10000~40000mg/L, VS 10000~
10000~40000mg/L of 30000mg/L, SS；VSS10000~30000mg/L, NH4- N 100~150mg/L, VFAs 20
~100mg/L.
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