CN109619093B - Low-temperature freezing living body quality guarantee method - Google Patents
Low-temperature freezing living body quality guarantee method Download PDFInfo
- Publication number
- CN109619093B CN109619093B CN201810380921.3A CN201810380921A CN109619093B CN 109619093 B CN109619093 B CN 109619093B CN 201810380921 A CN201810380921 A CN 201810380921A CN 109619093 B CN109619093 B CN 109619093B
- Authority
- CN
- China
- Prior art keywords
- temperature
- cooling
- low
- speed
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
Abstract
The invention belongs to the technical field of freezing preservation, and provides a low-temperature freezing living body quality guarantee method, which comprises the following steps: A. material pretreatment: removing soil and impurities on the surface of fresh plant rootstocks; B. cooling and dehydrating: under the ventilation condition, carrying out gradient cooling treatment on the fresh plant rootstocks in the step A to obtain low-temperature dehydrated plant rootstocks below 0 ℃, and adopting the slowest cooling speed to be less than 1.0 ℃/d when the temperature is reduced to 4 ℃; C. water spraying, ventilation and preservation: keeping the temperature of the low-temperature dehydrated plant roots and stems in the step B constant, providing air for the low-temperature dehydrated plant roots and stems, spraying water mist according to a period, and forming a heat-preservation water-replenishing ice layer on the surface of the water mist to obtain frozen living plant roots and stems; D. temperature rise and water supplement treatment: and C, under the ventilation condition, carrying out gradient heating treatment on the frozen living plant rhizomes in the step C to obtain quality-guaranteeing plant rhizomes. The invention solves the problem that the traditional refrigeration technology can not realize the re-cultivation of the plant rootstocks after long-time refrigeration.
Description
Technical Field
The invention belongs to the technical field of freezing preservation, and relates to a quality guarantee method for a low-temperature frozen living body.
Background
The traditional refrigeration technology can only realize short-term preservation and preservation of plant branches, fruits, seeds, rhizomes, leaves and the like, the plants can wither and even rot when preserved for a long time, and particularly the roots and the stalks of the plants can still keep living after the long-term refrigeration and can be used for secondary cultivation. The reason is that the plant cells contain a large amount of water, the density and the volume of water at 4 ℃ are the maximum and the volume are the minimum, the water volume is increased by the increase and the decrease of the temperature, and most of the traditional refrigeration technologies are used for rapidly cooling and freezing the plants under the closed and non-ventilated conditions, so that the free water in the plant cells cannot be lost, the frozen volume is increased, the cells are punctured, the structure of the plant cells is damaged irreversibly, and the integrity of the cell structure is an important condition for quality guarantee and preservation activity.
Disclosure of Invention
The invention provides a quality guarantee method for a low-temperature frozen living body, which solves the technical problems.
A method for preserving quality of a low-temperature frozen living body is characterized by comprising the following steps:
A. material pretreatment: removing soil on the surface of fresh plant rootstocks;
B. cooling and dehydrating: under the ventilation condition, carrying out gradient cooling treatment on the fresh plant rootstocks in the step A to obtain low-temperature dehydrated plant rootstocks below 0 ℃, and adopting the slowest cooling speed to be less than 1.0 ℃/d when the temperature is reduced to 4 ℃;
C. water spraying, ventilation and preservation: keeping the temperature of the low-temperature dehydrated plant roots and stems in the step B constant, providing air for the low-temperature dehydrated plant roots and stems, spraying water mist according to a period, and forming a heat-preservation water-replenishing ice layer on the surface of the water mist to obtain frozen living plant roots and stems;
D. temperature rise and water supplement treatment: and C, under the ventilation condition, carrying out gradient heating treatment on the frozen living plant rhizomes in the step C to obtain quality-guaranteeing plant rhizomes, wherein the slowest heating speed is adopted when the gradient heating is carried out at 4 ℃ and is less than 1.0 ℃/d.
As a further technical scheme, the ventilation speed in the step B is kept constant, the gradient cooling is divided into primary cooling which is higher than 5 ℃, secondary cooling which is 5-0 ℃ and tertiary cooling which is lower than 0 ℃, the primary cooling speed is higher than the tertiary cooling speed, and the tertiary cooling speed is higher than the secondary cooling speed.
As a further technical scheme, the ventilation speed in the step B is 0.1-0.3 m/s, the secondary cooling speed is 0.1-0.9 ℃/d, and the tertiary cooling speed is 1.0-2.0 ℃/d.
And C, as a further technical scheme, the air in the step C is fresh air, the thickness of the heat-preservation water-replenishing ice layer is 0.1-2.0 mm, and the period of spraying water mist is adjusted according to the thickness of the heat-preservation water-replenishing ice layer.
As a further technical scheme, the ventilation speed in the step D is kept constant and is 0.2 m/s-0.4 m/s, the gradient temperature rise is divided into a first-stage temperature rise of less than 0 ℃, a second-stage temperature rise of 0-5 ℃ and a third-stage temperature rise of more than 5 ℃, the second-stage temperature rise speed is 0.1 ℃/D-0.9 ℃/D, the first-stage temperature rise speed is 1.0 ℃/D-2.0 ℃/D, and the third-stage temperature rise is natural temperature rise.
As a further technical scheme, the fresh plant rhizome is chicory root, the speed of secondary cooling is 0.5 ℃/d, the speed of tertiary cooling is 1.5 ℃/d, the storage time is 1 year when the final temperature of gradient cooling is-8 ℃, the storage time is 0.5 year when the final temperature of gradient cooling is-3 ℃ to-5 ℃, the thickness of the ice layer is 1mm, and the period of spraying water mist is once every three days.
As a further technical scheme, in the process of gradient temperature rise in the step D, fresh air is continuously introduced into the frozen live plant rhizomes, and magnetized water is intermittently sprayed at the same time, wherein the flow rate of the fresh air is 0.2 m/s.
As a further technical scheme, in the process of gradient temperature rise in the step D, oxygen-containing water is sprayed to the roots and stems of the frozen living plants to carry out water supplementing and oxygen supplementing treatment, wherein the spraying period of the oxygen-containing water is once a day.
As a further technical scheme, in the step C, the frozen living plant rhizomes are subjected to illumination treatment in the process of spraying water mist, the frozen living plant rhizomes are not subjected to illumination when the water mist is not sprayed, and in the step D, the gradient illumination treatment is performed when the gradient temperature rise treatment is performed.
As a further technical scheme, the gradient illumination comprises primary illumination, secondary illumination and tertiary illumination with sequentially enhanced illumination intensity, wherein the illumination intensity of the primary illumination, the secondary illumination and the tertiary illumination is sequentially 10 Lux-30 Lux, 40 Lux-60 Lux and 60 Lux-100 Lux.
The principle and the beneficial effects of the invention are as follows:
1. in the invention, the plant roots and stems are subjected to gradient cooling under the ventilation condition, and the ventilation and the gradient cooling are beneficial to slowly removing water from the plant roots and stems in the cooling process; meanwhile, the maximum density and the minimum volume of water at 4 ℃ are the minimum, so that the volume change of the plant cells at 4 ℃ is large, if the plant cells are irreversibly damaged by adopting rapid cooling at 4 ℃, the cooling speed is slowest and is less than 1.0 ℃/d by adopting gradient cooling at 4 ℃, so that the damage and inactivation of the plant cell structure can be avoided, the plant cell structure is ensured to be intact, and the quality guarantee and the preservation activity of plant rhizomes are facilitated.
2. In the invention, the low-temperature dehydrated plant rootstocks are preserved under the condition of water spraying, ventilation, namely fresh air is provided for the low-temperature dehydrated plant rootstocks, so that the basic respiration of the low-temperature dehydrated plant rootstocks is ensured, and the plant rot is avoided; regularly spray water smoke and form heat preservation moisturizing ice sheet on its surface, the ice sheet is favorable to keeping the low temperature invariable on the one hand, and on the other hand can be to the necessary moisture of low temperature dehydration plant rhizome supplementary life activity, and the ice sheet is too thin to be favorable to keeping warm and moisturizing, and the ice sheet is too thick to be favorable to the plant respiration to go on, and suitable ice sheet thickness guarantees that the plant is in the frozen state of living body, the time of extension plant rhizome living body preservation.
3. According to the invention, the frozen living plant rootstocks are subjected to gradient temperature rise under the condition of ventilation, namely oxygen supply, wherein the temperature rise speed is slowest and less than 1.0 ℃/d when the temperature is raised to 4 ℃, the slow gradient temperature rise is favorable for the frozen living plant rootstocks to slowly absorb water melted by an ice layer in the thawing process, the plant cells are gradually restored to a normal water-containing state, the damage to the plant cell structure caused by too fast temperature rise is avoided, and the cultivation of the thawed plant living bodies is facilitated.
4. In the invention, under the condition that the ventilation condition, the cooling speed of gradient cooling, the heating speed of gradient heating and the thickness of a heat-preservation water-replenishing ice layer are all suitable, the living body preservation time of plant rootstocks can be adjusted by changing the freezing preservation temperature of frozen living plant rootstocks, for example, under the ventilation condition, the speed of secondary cooling is 0.5 ℃/d, the speed of tertiary cooling is 1.5 ℃/d, the thickness of the ice layer is 1mm, the preservation time of chicory roots is about 1 year when the final temperature of gradient cooling is-8 ℃, the thawed chicory roots can be used for processing and producing chicory tea, coffee substitutes and traditional Chinese medicine decoction pieces, meanwhile, the thawed chicory roots can be used for producing chicory in a water culture mode, and the preservation time is about half a year when the final temperature of gradient cooling is-3 ℃ to-5 ℃.
5. In the invention, the step C of storing by spraying water has the influence on the living body storage time and the living body cultivation survival rate by adopting the steps of spraying common water, magnetized water and oxygen-containing water, wherein compared with the step C of spraying common water, the step C of spraying the magnetized water and the oxygen-containing water is favorable for prolonging the living body storage time of the chicory roots and improving the living body cultivation survival rate of the chicory roots; meanwhile, compared with the direct illumination, the gradient illumination adopted in the temperature rise water replenishing step D can improve the survival rate of the living body cultivation.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A method for preserving quality of a low-temperature frozen living body is characterized by comprising the following steps:
A. material pretreatment: removing soil and impurities on the surface of the chicory root;
B. cooling and dehydrating: b, while keeping the ventilation air speed at 0.1m/s, carrying out gradient cooling treatment on the chicory root in the step A, firstly cooling the chicory root from room temperature to 5 ℃ at a cooling speed of 10 ℃/d, then cooling the chicory root from 5 ℃ to 0 ℃ at a cooling speed of 0.1 ℃/d, and then cooling the chicory root from 0 ℃ to-3 ℃ at a cooling speed of 1.0 ℃/d to obtain low-temperature dehydrated chicory root;
C. water spraying, ventilation and preservation: keeping the temperature of the low-temperature dehydrated chicory roots in the step B constant at-3 ℃, providing air for the low-temperature dehydrated chicory roots, spraying water mist on the low-temperature dehydrated chicory roots once every three days at the same time with the air flow rate of 0.2m/s, and forming a heat-preservation water-replenishing ice layer with the thickness of 0.1mm on the surface of the water mist to obtain frozen living chicory roots;
D. temperature rise and water supplement treatment: and C, while keeping the ventilation air speed at 0.2m/s, carrying out gradient temperature rise on the frozen living chicory root in the step C, namely, raising the temperature from-3 ℃ to 0 ℃ at the temperature rise speed of 1.0 ℃/d, then raising the temperature from 0 ℃ to 5 ℃ at the temperature rise speed of 0.1 ℃/d, and finally raising the temperature from 5 ℃ to room temperature at the temperature rise speed of 10 ℃/d to obtain the quality-guaranteed chicory root.
Example 2
A method for preserving quality of a low-temperature frozen living body is characterized by comprising the following steps:
A. material pretreatment: removing soil on the surface of the chicory root, and removing yellow leaves;
B. cooling and dehydrating: b, while keeping the ventilation air speed at 0.1m/s, carrying out gradient cooling treatment on the chicory root in the step A, firstly cooling the chicory root from room temperature to 5 ℃ at a cooling speed of 10 ℃/d, then cooling the chicory root from 5 ℃ to 0 ℃ at a cooling speed of 0.5 ℃/d, and then cooling the chicory root from 0 ℃ to-3 ℃ at a cooling speed of 1.5 ℃/d to obtain low-temperature dehydrated chicory root;
C. water spraying, ventilation and preservation: keeping the temperature of the low-temperature dehydrated chicory roots in the step B constant at-3 ℃, providing air for the low-temperature dehydrated chicory roots, spraying water mist on the low-temperature dehydrated chicory roots once every three days at the same time with the air flow rate of 0.2m/s, and forming a heat-preservation water-replenishing ice layer with the thickness of 0.1mm on the surface of the water mist to obtain frozen living chicory roots;
D. temperature rise and water supplement treatment: and C, while keeping the ventilation air speed at 0.2m/s, carrying out gradient temperature rise on the frozen living chicory root in the step C, namely, raising the temperature from-3 ℃ to 0 ℃ at the temperature rise speed of 1.0 ℃/d, then raising the temperature from 0 ℃ to 5 ℃ at the temperature rise speed of 0.1 ℃/d, and finally raising the temperature from 5 ℃ to room temperature at the temperature rise speed of 10 ℃/d to obtain the quality-guaranteed chicory root.
Example 3
A method for preserving quality of a low-temperature frozen living body is characterized by comprising the following steps:
A. material pretreatment: removing soil and impurities on the surface of the chicory root;
B. cooling and dehydrating: b, while keeping the ventilation air speed at 0.1m/s, carrying out gradient cooling treatment on the chicory root in the step A, firstly cooling the chicory root from room temperature to 5 ℃ at a cooling speed of 10 ℃/d, then cooling the chicory root from 5 ℃ to 0 ℃ at a cooling speed of 0.5 ℃/d, and then cooling the chicory root from 0 ℃ to-3 ℃ at a cooling speed of 2.0 ℃/d to obtain low-temperature dehydrated chicory root;
C. water spraying, ventilation and preservation: keeping the temperature of the low-temperature dehydrated chicory roots in the step B constant at-3 ℃, providing air for the low-temperature dehydrated chicory roots, spraying water mist on the low-temperature dehydrated chicory roots once every three days at the same time with the air flow rate of 0.2m/s, and forming a heat-preservation water-replenishing ice layer with the thickness of 0.1mm on the surface of the water mist to obtain frozen living chicory roots;
D. temperature rise and water supplement treatment: and C, while keeping the ventilation air speed at 0.2m/s, carrying out gradient temperature rise on the frozen living chicory root in the step C, namely, raising the temperature from-3 ℃ to 0 ℃ at the temperature rise speed of 1.0 ℃/d, then raising the temperature from 0 ℃ to 5 ℃ at the temperature rise speed of 0.1 ℃/d, and finally raising the temperature from 5 ℃ to room temperature at the temperature rise speed of 10 ℃/d to obtain the quality-guaranteed chicory root.
Example 4
A method for preserving quality of a low-temperature frozen living body is characterized by comprising the following steps:
A. material pretreatment: removing soil and impurities on the surface of the chicory root;
B. cooling and dehydrating: b, while keeping the ventilation air speed at 0.1m/s, carrying out gradient cooling treatment on the chicory root in the step A, firstly cooling the chicory root from room temperature to 5 ℃ at a cooling speed of 10 ℃/d, then cooling the chicory root from 5 ℃ to 0 ℃ at a cooling speed of 0.9 ℃/d, and then cooling the chicory root from 0 ℃ to-3 ℃ at a cooling speed of 2.0 ℃/d to obtain low-temperature dehydrated chicory root;
C. water spraying, ventilation and preservation: keeping the temperature of the low-temperature dehydrated chicory roots in the step B constant at-3 ℃, providing air for the low-temperature dehydrated chicory roots, spraying water mist on the low-temperature dehydrated chicory roots once every three days at the same time with the air flow rate of 0.2m/s, and forming a heat-preservation water-replenishing ice layer with the thickness of 0.1mm on the surface of the water mist to obtain frozen living chicory roots;
D. temperature rise and water supplement treatment: and C, while keeping the ventilation air speed at 0.2m/s, carrying out gradient temperature rise on the frozen living chicory root in the step C, namely, raising the temperature from-3 ℃ to 0 ℃ at the temperature rise speed of 1.0 ℃/d, then raising the temperature from 0 ℃ to 5 ℃ at the temperature rise speed of 0.1 ℃/d, and finally raising the temperature from 5 ℃ to room temperature at the temperature rise speed of 10 ℃/d to obtain the quality-guaranteed chicory root.
Example 5
A method for preserving quality of a low-temperature frozen living body is characterized by comprising the following steps:
A. material pretreatment: removing soil and impurities on the surface of the chicory root;
B. cooling and dehydrating: b, while keeping the ventilation air speed at 0.1m/s, carrying out gradient cooling treatment on the chicory root in the step A, firstly cooling the chicory root from room temperature to 5 ℃ at a cooling speed of 10 ℃/d, then cooling the chicory root from 5 ℃ to 0 ℃ at a cooling speed of 0.5 ℃/d, and then cooling the chicory root from 0 ℃ to-3 ℃ at a cooling speed of 1.5 ℃/d to obtain low-temperature dehydrated chicory root;
C. water spraying, ventilation and preservation: keeping the temperature of the low-temperature dehydrated chicory roots in the step B constant at-3 ℃, providing air for the low-temperature dehydrated chicory roots, spraying water mist on the low-temperature dehydrated chicory roots once every three days at the same time with the air flow rate of 0.2m/s, and forming a heat-preservation water-replenishing ice layer with the thickness of 0.1mm on the surface of the water mist to obtain frozen living chicory roots;
D. temperature rise and water supplement treatment: and C, while keeping the ventilation air speed at 0.2m/s, carrying out gradient temperature rise on the frozen living chicory root in the step C, namely, raising the temperature from-3 ℃ to 0 ℃ at the temperature rise speed of 1.5 ℃/d, then raising the temperature from 0 ℃ to 5 ℃ at the temperature rise speed of 0.5 ℃/d, and finally raising the temperature from 5 ℃ to room temperature at the temperature rise speed of 10 ℃/d to obtain the quality-guaranteed chicory root.
Example 6
A method for preserving quality of a low-temperature frozen living body is characterized by comprising the following steps:
A. material pretreatment: removing soil and impurities on the surface of the chicory root;
B. cooling and dehydrating: b, while keeping the ventilation air speed at 0.3m/s, carrying out gradient cooling treatment on the chicory root in the step A, firstly cooling the chicory root from room temperature to 5 ℃ at a cooling speed of 10 ℃/d, then cooling the chicory root from 5 ℃ to 0 ℃ at a cooling speed of 0.5 ℃/d, and then cooling the chicory root from 0 ℃ to-5 ℃ at a cooling speed of 1.5 ℃/d to obtain low-temperature dehydrated chicory root;
C. water spraying, ventilation and preservation: keeping the temperature of the low-temperature dehydrated chicory roots in the step B constant at-5 ℃, providing air for the low-temperature dehydrated chicory roots, spraying water mist on the low-temperature dehydrated chicory roots once every three days at the same time with the air flow rate of 0.2m/s, and forming a heat-preservation water-replenishing ice layer with the thickness of 1.0mm on the surface of the water mist to obtain frozen living chicory roots;
D. temperature rise and water supplement treatment: and C, while keeping the ventilation air speed at 0.5m/s, carrying out gradient temperature rise on the frozen living chicory root in the step C, namely, raising the temperature from-5 ℃ to 0 ℃ at the temperature rise speed of 1.5 ℃/d, then raising the temperature from 0 ℃ to 5 ℃ at the temperature rise speed of 0.5 ℃/d, and finally raising the temperature from 5 ℃ to room temperature at the temperature rise speed of 10 ℃/d to obtain the quality-guaranteed chicory root.
Example 7
A method for preserving quality of a low-temperature frozen living body is characterized by comprising the following steps:
A. material pretreatment: removing soil and impurities on the surface of the chicory root;
B. cooling and dehydrating: b, while keeping the ventilation air speed at 0.3m/s, carrying out gradient cooling treatment on the chicory root in the step A, firstly cooling the chicory root from room temperature to 5 ℃ at a cooling speed of 5 ℃/d, then cooling the chicory root from 5 ℃ to 0 ℃ at a cooling speed of 0.5 ℃/d, and then cooling the chicory root from 0 ℃ to-5 ℃ at a cooling speed of 1.5 ℃/d to obtain low-temperature dehydrated chicory root;
C. water spraying, ventilation and preservation: keeping the temperature of the low-temperature dehydrated chicory roots in the step B constant at-5 ℃, providing air for the low-temperature dehydrated chicory roots, spraying water mist on the low-temperature dehydrated chicory roots once every three days at the same time with the air flow rate of 0.2m/s, and forming a heat-preservation water-replenishing ice layer with the thickness of 1.0mm on the surface of the water mist to obtain frozen living chicory roots;
D. temperature rise and water supplement treatment: and C, while keeping the ventilation air speed at 0.5m/s, carrying out gradient temperature rise on the frozen living chicory root in the step C, namely, raising the temperature from-5 ℃ to 0 ℃ at the temperature rise speed of 1.5 ℃/d, then raising the temperature from 0 ℃ to 5 ℃ at the temperature rise speed of 0.5 ℃/d, and finally raising the temperature from 5 ℃ to room temperature at the temperature rise speed of 5 ℃/d to obtain the quality-guaranteed chicory root.
Example 8
A method for preserving quality of a low-temperature frozen living body is characterized by comprising the following steps:
A. material pretreatment: removing soil on the surface of the chicory root, and removing yellow leaves;
B. cooling and dehydrating: b, while keeping the ventilation air speed at 0.3m/s, carrying out gradient cooling treatment on the chicory root in the step A, firstly cooling the chicory root from room temperature to 5 ℃ at a cooling speed of 5 ℃/d, then cooling the chicory root from 5 ℃ to 0 ℃ at a cooling speed of 0.5 ℃/d, and then cooling the chicory root from 0 ℃ to-5 ℃ at a cooling speed of 1.5 ℃/d to obtain low-temperature dehydrated chicory root;
C. water spraying, ventilation and preservation: keeping the temperature of the low-temperature dehydrated chicory roots in the step B constant at-5 ℃, providing air for the low-temperature dehydrated chicory roots, spraying water mist on the low-temperature dehydrated chicory roots once every three days at the same time with the air flow rate of 0.2m/s, and forming a heat-preservation water-replenishing ice layer with the thickness of 1.5mm on the surface of the water mist to obtain frozen living chicory roots;
D. temperature rise and water supplement treatment: and C, while keeping the ventilation air speed at 0.5m/s, carrying out gradient temperature rise on the frozen living chicory root in the step C, namely, raising the temperature from-5 ℃ to 0 ℃ at the temperature rise speed of 1.5 ℃/d, then raising the temperature from 0 ℃ to 5 ℃ at the temperature rise speed of 0.5 ℃/d, and finally raising the temperature from 5 ℃ to room temperature at the temperature rise speed of 5 ℃/d to obtain the quality-guaranteed chicory root.
Example 9
A method for preserving quality of a low-temperature frozen living body is characterized by comprising the following steps:
A. material pretreatment: removing soil and impurities on the surface of the chicory root;
B. cooling and dehydrating: b, while keeping the ventilation air speed at 0.3m/s, carrying out gradient cooling treatment on the chicory root in the step A, firstly cooling the chicory root from room temperature to 5 ℃ at a cooling speed of 5 ℃/d, then cooling the chicory root from 5 ℃ to 0 ℃ at a cooling speed of 0.5 ℃/d, and then cooling the chicory root from 0 ℃ to-5 ℃ at a cooling speed of 1.5 ℃/d to obtain low-temperature dehydrated chicory root;
C. water spraying, ventilation and preservation: keeping the temperature of the low-temperature dehydrated chicory roots in the step B constant at-5 ℃, providing air for the low-temperature dehydrated chicory roots, spraying water mist on the low-temperature dehydrated chicory roots once every three days at the same time with the air flow rate of 0.2m/s, and forming a heat-preservation water-replenishing ice layer with the thickness of 1.0mm on the surface of the water mist to obtain frozen living chicory roots;
D. temperature rise and water supplement treatment: and C, while keeping the ventilation air speed at 0.5m/s, carrying out gradient temperature rise on the frozen living chicory root in the step C, namely, raising the temperature from-5 ℃ to 0 ℃ at the temperature rise speed of 1.5 ℃/d, then raising the temperature from 0 ℃ to 5 ℃ at the temperature rise speed of 0.9 ℃/d, and finally raising the temperature from 5 ℃ to room temperature at the temperature rise speed of 5 ℃/d to obtain the quality-guaranteed chicory root.
Example 10
A method for preserving quality of a low-temperature frozen living body is characterized by comprising the following steps:
A. material pretreatment: removing soil and impurities on the surface of the chicory root;
B. cooling and dehydrating: b, while keeping the ventilation air speed at 0.3m/s, carrying out gradient cooling treatment on the chicory root in the step A, firstly cooling the chicory root from room temperature to 5 ℃ at a cooling speed of 5 ℃/d, then cooling the chicory root from 5 ℃ to 0 ℃ at a cooling speed of 0.5 ℃/d, and then cooling the chicory root from 0 ℃ to-5 ℃ at a cooling speed of 1.5 ℃/d to obtain low-temperature dehydrated chicory root;
C. water spraying, ventilation and preservation: keeping the temperature of the low-temperature dehydrated chicory roots in the step B constant at-5 ℃, providing air for the low-temperature dehydrated chicory roots, spraying water mist on the low-temperature dehydrated chicory roots once every three days at the same time with the air flow rate of 0.2m/s, and forming a heat-preservation water-replenishing ice layer with the thickness of 1.0mm on the surface of the water mist to obtain frozen living chicory roots;
D. temperature rise and water supplement treatment: and C, while keeping the ventilation air speed at 0.5m/s, carrying out gradient temperature rise on the frozen living chicory root in the step C, namely, raising the temperature from-5 ℃ to 0 ℃ at the temperature rise speed of 2.0 ℃/d, then raising the temperature from 0 ℃ to 5 ℃ at the temperature rise speed of 0.9 ℃/d, and finally raising the temperature from 5 ℃ to room temperature at the temperature rise speed of 5 ℃/d to obtain the quality-guaranteed chicory root.
Example 11
A method for preserving quality of a low-temperature frozen living body is characterized by comprising the following steps:
A. material pretreatment: removing soil and impurities on the surface of the chicory root;
B. cooling and dehydrating: b, while keeping the ventilation air speed at 0.3m/s, carrying out gradient cooling treatment on the chicory root in the step A, firstly cooling the chicory root from room temperature to 5 ℃ at a cooling speed of 5 ℃/d, then cooling the chicory root from 5 ℃ to 0 ℃ at a cooling speed of 0.5 ℃/d, and then cooling the chicory root from 0 ℃ to-5 ℃ at a cooling speed of 1.5 ℃/d to obtain low-temperature dehydrated chicory root;
C. water spraying, ventilation and preservation: keeping the temperature of the low-temperature dehydrated chicory roots in the step B constant at-5 ℃, providing air for the low-temperature dehydrated chicory roots, spraying water mist on the low-temperature dehydrated chicory roots once every three days at the same time with the air flow rate of 0.4m/s, and forming a heat-preservation water-replenishing ice layer with the thickness of 1.0mm on the surface of the water mist to obtain frozen living chicory roots;
D. temperature rise and water supplement treatment: and C, while keeping the ventilation air speed at 0.5m/s, carrying out gradient temperature rise on the frozen living chicory root in the step C, namely, raising the temperature from-5 ℃ to 0 ℃ at the temperature rise speed of 1.5 ℃/d, then raising the temperature from 0 ℃ to 5 ℃ at the temperature rise speed of 0.5 ℃/d, and finally raising the temperature from 5 ℃ to room temperature at the temperature rise speed of 5 ℃/d to obtain the quality-guaranteed chicory root.
Example 12
A method for preserving quality of a low-temperature frozen living body is characterized by comprising the following steps:
A. material pretreatment: removing soil and impurities on the surface of the chicory root;
B. cooling and dehydrating: b, while keeping the ventilation air speed at 0.3m/s, carrying out gradient cooling treatment on the chicory root in the step A, firstly cooling the chicory root from room temperature to 5 ℃ at a cooling speed of 5 ℃/d, then cooling the chicory root from 5 ℃ to 0 ℃ at a cooling speed of 0.5 ℃/d, and then cooling the chicory root from 0 ℃ to-8 ℃ at a cooling speed of 1.5 ℃/d to obtain low-temperature dehydrated chicory root;
C. water spraying, ventilation and preservation: keeping the temperature of the low-temperature dehydrated chicory roots in the step B constant at-8 ℃, providing air for the low-temperature dehydrated chicory roots, spraying water mist on the low-temperature dehydrated chicory roots once every three days at the same time with the air flow rate of 0.2m/s, and forming a heat-preservation water-replenishing ice layer with the thickness of 1.0mm on the surface of the water mist to obtain frozen living chicory roots;
D. temperature rise and water supplement treatment: and C, while keeping the ventilation air speed at 0.5m/s, carrying out gradient temperature rise on the frozen living chicory root in the step C, namely, raising the temperature from-8 ℃ to 0 ℃ at the temperature rise speed of 1.5 ℃/d, then raising the temperature from 0 ℃ to 5 ℃ at the temperature rise speed of 0.5 ℃/d, and finally raising the temperature from 5 ℃ to room temperature at the temperature rise speed of 5 ℃/d to obtain the quality-guaranteed chicory root.
Example 13
A method for preserving quality of a low-temperature frozen living body is characterized by comprising the following steps:
A. material pretreatment: removing soil and impurities on the surface of the chicory root;
B. cooling and dehydrating: b, while keeping the ventilation air speed at 0.3m/s, carrying out gradient cooling treatment on the chicory root in the step A, firstly cooling the chicory root from room temperature to 5 ℃ at a cooling speed of 5 ℃/d, then cooling the chicory root from 5 ℃ to 0 ℃ at a cooling speed of 0.5 ℃/d, and then cooling the chicory root from 0 ℃ to-10 ℃ at a cooling speed of 1.5 ℃/d to obtain low-temperature dehydrated chicory root;
C. water spraying, ventilation and preservation: keeping the temperature of the low-temperature dehydrated chicory roots in the step B constant at-10 ℃, providing air for the low-temperature dehydrated chicory roots, spraying water mist on the low-temperature dehydrated chicory roots once every three days at the same time with the air flow rate of 0.2m/s, and forming a heat-preservation water-replenishing ice layer with the thickness of 1.0mm on the surface of the water mist to obtain frozen living chicory roots;
D. temperature rise and water supplement treatment: and C, while keeping the ventilation air speed at 0.5m/s, carrying out gradient temperature rise on the frozen living chicory root in the step C, namely, raising the temperature from-10 ℃ to 0 ℃ at the temperature rise speed of 1.5 ℃/d, then raising the temperature from 0 ℃ to 5 ℃ at the temperature rise speed of 0.5 ℃/d, and finally raising the temperature from 5 ℃ to room temperature at the temperature rise speed of 5 ℃/d to obtain the quality-guaranteed chicory root.
Example 14
A method for preserving quality of a low-temperature frozen living body is characterized by comprising the following steps:
A. material pretreatment: removing soil and impurities on the surface of the chicory root;
B. cooling and dehydrating: c, while keeping the ventilation air speed at 0.3m/s, carrying out gradient cooling treatment on the chicory root in the step A, and firstly cooling from room temperature to-5 ℃ at the cooling speed of 5 ℃/d to obtain low-temperature dehydrated chicory root;
C. water spraying, ventilation and preservation: keeping the temperature of the low-temperature dehydrated chicory roots in the step B constant at-5 ℃, providing air for the low-temperature dehydrated chicory roots, spraying water mist on the low-temperature dehydrated chicory roots once every three days at the same time with the air flow rate of 0.2m/s, and forming a heat-preservation water-replenishing ice layer with the thickness of 1.0 on the surface of the water mist to obtain frozen living chicory roots;
D. temperature rise and water supplement treatment: and C, while keeping the ventilation air speed at 0.5m/s, carrying out gradient temperature rise on the frozen living chicory root in the step C, namely, raising the temperature from-5 ℃ to 0 ℃ at the temperature rise speed of 1.5 ℃/d, then raising the temperature from 0 ℃ to 5 ℃ at the temperature rise speed of 0.5 ℃/d, and finally raising the temperature from 5 ℃ to room temperature at the temperature rise speed of 5 ℃/d to obtain the unfrozen chicory root.
Example 15
A method for preserving quality of a low-temperature frozen living body is characterized by comprising the following steps:
A. material pretreatment: removing soil and impurities on the surface of the chicory root;
B. cooling and dehydrating: b, while keeping the ventilation air speed at 0.3m/s, carrying out gradient cooling treatment on the chicory root in the step A, firstly cooling the chicory root from room temperature to 5 ℃ at a cooling speed of 5 ℃/d, then cooling the chicory root from 5 ℃ to 0 ℃ at a cooling speed of 0.5 ℃/d, and then cooling the chicory root from 0 ℃ to-5 ℃ at a cooling speed of 1.5 ℃/d to obtain low-temperature dehydrated chicory root;
C. water spraying, ventilation and preservation: keeping the temperature of the low-temperature dehydrated chicory roots in the step B constant at-5 ℃, providing air for the low-temperature dehydrated chicory roots, spraying water mist on the low-temperature dehydrated chicory roots once every three days at the same time with the air flow rate of 0.2m/s, and forming a heat-preservation water-replenishing ice layer with the thickness of 1.0mm on the surface of the low-temperature dehydrated chicory roots to obtain frozen chicory roots;
D. temperature rise and water supplement treatment: and C, heating the frozen living chicory root in the step C at a ventilation speed of 0.5m/s, namely heating the frozen living chicory root from-5 ℃ to room temperature at a heating speed of 5 ℃/d to obtain the unfrozen chicory root.
Example 16
A method for preserving quality of a low-temperature frozen living body is characterized by comprising the following steps:
A. material pretreatment: removing soil and impurities on the surface of the chicory root;
B. cooling and dehydrating: c, while keeping the ventilation air speed at 0.3m/s, carrying out gradient cooling treatment on the chicory root in the step A, and firstly cooling from room temperature to-8 ℃ at the cooling speed of 10 ℃/d to obtain low-temperature dehydrated chicory root;
C. water spraying, ventilation and preservation: keeping the temperature of the low-temperature dehydrated chicory roots in the step B constant at-8 ℃, providing air for the low-temperature dehydrated chicory roots, spraying water mist on the low-temperature dehydrated chicory roots once every three days at the same time with the air flow rate of 0.2m/s, and forming a heat-preservation water-replenishing ice layer with the thickness of 1.0mm on the surface of the low-temperature dehydrated chicory roots to obtain frozen chicory roots;
D. temperature rise and water supplement treatment: and C, while keeping the ventilation air speed at 0.5m/s, carrying out gradient temperature rise on the frozen living chicory root in the step C, namely, raising the temperature from-8 ℃ to 0 ℃ at the temperature rise speed of 1.5 ℃/d, then raising the temperature from 0 ℃ to 5 ℃ at the temperature rise speed of 0.5 ℃/d, and finally raising the temperature from 5 ℃ to room temperature at the temperature rise speed of 5 ℃/d to obtain the unfrozen chicory root.
Example 17
A method for preserving quality of a low-temperature frozen living body is characterized by comprising the following steps:
A. material pretreatment: removing soil and impurities on the surface of the chicory root;
B. cooling treatment: c, under the condition of no ventilation, carrying out gradient cooling treatment on the chicory root in the step A, firstly cooling from room temperature to 5 ℃ at a cooling speed of 5 ℃/d, then cooling from 5 ℃ to 0 ℃ at a cooling speed of 0.5 ℃/d, and then cooling from 0 ℃ to-5 ℃ at a cooling speed of 1.5 ℃/d to obtain low-temperature chicory root;
C. and (4) water spraying preservation: keeping the temperature of the low-temperature chicory root in the step B constant at-5 ℃, spraying water mist on the chicory root once in three days under the condition of no ventilation, and forming a heat-preservation water-replenishing ice layer with the thickness of 1.0mm on the surface of the chicory root to obtain frozen chicory root;
D. temperature rise and water supplement treatment: and C, under the condition of no ventilation, carrying out gradient temperature rise on the frozen chicory root in the step C, namely, raising the temperature from-5 ℃ to 0 ℃ at the temperature rise speed of 1.5 ℃/d, then raising the temperature from 0 ℃ to 5 ℃ at the temperature rise speed of 0.5 ℃/d, and finally raising the temperature from 5 ℃ to room temperature at the temperature rise speed of 5 ℃/d to obtain the unfrozen chicory root.
According to the embodiments 1-17, 1000 chicory roots are respectively subjected to living body preservation, and then the living body preservation time is tested, and the test results show that the ventilation condition, the gradient cooling rate, the gradient heating rate and the thickness of the heat-preservation water-replenishing ice layer all have important influence on the living body preservation time of the chicory roots. Through comparison between examples 1 to 13 and examples 14 to 17, it is known that the living storage time of the chicory root is greatly shortened due to no ventilation, no gradient temperature rise and drop, or too fast temperature rise and drop speed, and too thin or too thick ice layer; the examples 1-13 show that the ventilation speed does not directly influence the living preservation time of the chicory roots, but the ventilation can provide oxygen, slowly take away water, and is beneficial to the slow dehydration and respiration of plants, so that the living preservation time of the chicory roots is indirectly influenced; as can be seen from examples 7 and 12, under the ventilation condition, the speed of the secondary cooling is 0.5 ℃/d, the speed of the tertiary cooling is 1.5 ℃/d, the thickness of the ice layer is 1mm, the preservation time is about 1 year when the final temperature of the gradient cooling is-8 ℃, the processing and the production of chicory tea, coffee substitutes and traditional Chinese medicine decoction pieces can be realized after the thawing, meanwhile, the chicory rootstocks can be kept living, the chicory can be produced by water culture after the thawing, the preservation time is about half a year when the final temperature of the gradient cooling is-3 ℃ to-5 ℃, and the test results are shown in Table 1:
TABLE 1 data for examples 1-17
Example 18
A method for preserving quality of a frozen living body at a low temperature, which is characterized in that, in the step C, oxygen-containing water is sprayed on the surface of chicory root once in three days, and the rest is the same as the example 7.
Example 19
A method for preserving quality of a frozen living body at a low temperature, characterized in that, in the step C, oxygen-containing water is sprayed on the surface of chicory root once every three days, and the rest is the same as in example 12.
Example 20
A method for preserving living bodies by freezing at low temperature is characterized in that magnetized water is sprayed on the surfaces of the chicory roots once in three days in the step C, and the rest is the same as in example 7.
Example 21
A method for preserving quality of a living body by low-temperature freezing is characterized in that magnetized water is sprayed on the surface of chicory roots once every three days in the step C, and the rest is the same as the example 12.
Example 22
A low-temperature freezing living body quality guarantee method is characterized in that water mist is sprayed on the surfaces of chicory roots once in three days in the step C, the water mist is ordinary water, gradient illumination is carried out in the gradient temperature rise process, and the rest is the same as that in the example 7.
Example 23
A method for keeping quality of living bodies frozen at low temperature is characterized in that water mist is sprayed on the surfaces of the chicory roots once in three days in the step C, the water mist is ordinary water, gradient illumination is carried out in the gradient temperature rise process, and the rest is the same as that in the example 12.
Respectively preserving 1000 chicory roots in vivo according to embodiments 7, 12 and 18-23, and then testing the preservation time of the living bodies and the water culture survival rate of the thawed chicory roots, wherein the test results show that the preservation time of the living bodies and the survival rate of the living bodies are influenced by adopting the steps of spraying common water, magnetizing water and oxygen-containing water in the water spraying preservation step C, wherein compared with the step of spraying common water, the magnetized water and the oxygen-containing water are beneficial to prolonging the preservation time of the living bodies of the chicory roots and improving the survival rate of the chicory produced by the living body cultivation of the chicory roots; meanwhile, compared with the direct illumination, the gradient illumination adopted in the temperature rise water replenishing step D can improve the survival rate of the living body cultivation. The test results are shown in table 2:
TABLE 2 data for examples 17 to 16
To sum up: according to the method of the embodiments 1-13, the plants such as chicory roots can be frozen for a long time, the living preservation time of the plants can be adjusted according to requirements, the fresh plants can be supplied all the year round, and the quality, freshness and cultivation are convenient.
Meanwhile, the method is also suitable for storing the roots and stems of the plants such as radish, sweet potato, ginger and the like.
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions, improvements, etc. within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. A method for preserving quality of a low-temperature frozen living body is characterized by comprising the following steps:
A. material pretreatment: removing soil on the surface of fresh plant rootstocks;
B. cooling and dehydrating: under the ventilation condition, carrying out gradient cooling treatment on the fresh plant rootstocks in the step A to obtain low-temperature dehydrated plant rootstocks below 0 ℃, and adopting the slowest cooling speed to be less than 1.0 ℃/d when the temperature is reduced to 4 ℃;
C. water spraying, ventilation and preservation: keeping the temperature of the low-temperature dehydrated plant roots and stems in the step B constant, providing air for the low-temperature dehydrated plant roots and stems, spraying water mist according to a period, and forming a heat-preservation water-replenishing ice layer on the surface of the water mist to obtain frozen living plant roots and stems;
D. temperature rise and water supplement treatment: c, under the ventilation condition, carrying out gradient heating treatment on the frozen living plant rhizomes in the step C to obtain quality-guaranteeing plant rhizomes, wherein the slowest heating speed is adopted when the gradient heating is carried out at 4 ℃ and is less than 1.0 ℃/d;
c, performing illumination treatment on the frozen living plant rootstocks in the step C in the process of spraying water mist, not performing illumination when the water mist is not sprayed, and performing gradient illumination treatment when the gradient temperature rise treatment is performed in the step D;
c, spraying oxygen-containing water or magnetized water on the surface of the chicory root once in three days in the step C; and C, in the step C, the air is fresh air, the thickness of the heat-preservation water-replenishing ice layer is 0.1-2.0 mm, and the period of spraying water mist is adjusted according to the thickness of the heat-preservation water-replenishing ice layer.
2. The quality guarantee method of the low-temperature frozen living body according to claim 1, wherein the ventilation speed in the step B is kept constant, and the gradient cooling is divided into a primary cooling of more than 5 ℃, a secondary cooling of 5-0 ℃ and a tertiary cooling of less than 0 ℃, wherein the primary cooling is faster than the tertiary cooling, and the tertiary cooling is faster than the secondary cooling.
3. The quality guarantee method for the frozen living body at the low temperature according to claim 2, wherein the ventilation speed in the step B is 0.1-0.3 m/s, the speed of the secondary cooling is 0.1-0.9 ℃/d, and the speed of the tertiary cooling is 1.0-2.0 ℃/d.
4. The quality guarantee method for the frozen living body at the low temperature according to claim 3, wherein the ventilation speed in the step D is kept constant and is 0.2m/s to 0.4m/s, the gradient temperature rise is divided into a first-stage temperature rise of less than 0 ℃, a second-stage temperature rise of 0 ℃ to 5 ℃ and a third-stage temperature rise of more than 5 ℃, the second-stage temperature rise speed is 0.1 ℃/D to 0.9 ℃/D, the first-stage temperature rise speed is 1.0 ℃/D to 2.0 ℃/D, and the third-stage temperature rise is a natural temperature rise.
5. The quality guarantee method of the low-temperature frozen living body according to claim 4, wherein the fresh plant rhizome is chicory root, the speed of the secondary cooling is 0.5 ℃/d, the speed of the tertiary cooling is 1.5 ℃/d, the storage time of the gradient cooling final temperature is 1 year at-8 ℃, the storage time of the gradient cooling final temperature is 0.5 year at-3 ℃ to-5 ℃, the thickness of the ice layer is 1mm, and the spraying period of the gradient cooling is once every three days.
6. The quality guarantee method of the frozen living plant at low temperature according to claim 4 or 5, wherein fresh air is continuously introduced into the frozen living plant rhizomes during the gradient temperature rise in the step D, and simultaneously magnetized water is intermittently sprayed, wherein the flow rate of the fresh air is 0.2 m/s.
7. The quality guarantee method of the frozen living body at low temperature according to claim 4 or 5, wherein the oxygen-containing water is sprayed to the roots and stems of the frozen living body during the gradient temperature rise in the step D to perform the water and oxygen supplement treatment, and the spraying period of the oxygen-containing water is once a day.
8. The quality guarantee method for the frozen living body at the low temperature as claimed in claim 1, wherein the gradient illumination comprises a primary illumination, a secondary illumination and a tertiary illumination with sequentially enhanced illumination intensity, and the illumination intensity of the primary illumination, the secondary illumination and the tertiary illumination is sequentially 10 Lux-30 Lux, 40 Lux-60 Lux and 60 Lux-100 Lux.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810380921.3A CN109619093B (en) | 2018-04-25 | 2018-04-25 | Low-temperature freezing living body quality guarantee method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810380921.3A CN109619093B (en) | 2018-04-25 | 2018-04-25 | Low-temperature freezing living body quality guarantee method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109619093A CN109619093A (en) | 2019-04-16 |
| CN109619093B true CN109619093B (en) | 2022-01-25 |
Family
ID=66066221
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810380921.3A Active CN109619093B (en) | 2018-04-25 | 2018-04-25 | Low-temperature freezing living body quality guarantee method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109619093B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113796227A (en) * | 2021-09-17 | 2021-12-17 | 海宁格恩泰科技有限公司 | Low-temperature treatment method for improving plant productivity |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1178450A (en) * | 1995-03-15 | 1998-04-08 | 株式会社冰温 | Method of preserving food or the like in nonfrozen state in temperature zone below icing point |
| CN105285087A (en) * | 2015-11-17 | 2016-02-03 | 河北联兴佳垚农业科技有限公司 | Chicory root storage method |
| CN107242290A (en) * | 2017-07-18 | 2017-10-13 | 宁夏中玺枣业股份有限公司 | Lingwu Long Jujube Inappropriate ADH syndrome pit disease forecooling method |
| CN107535483A (en) * | 2017-08-15 | 2018-01-05 | 合肥元政农林生态科技有限公司 | A kind of storage method of hare's-lettuce seed |
-
2018
- 2018-04-25 CN CN201810380921.3A patent/CN109619093B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1178450A (en) * | 1995-03-15 | 1998-04-08 | 株式会社冰温 | Method of preserving food or the like in nonfrozen state in temperature zone below icing point |
| CN105285087A (en) * | 2015-11-17 | 2016-02-03 | 河北联兴佳垚农业科技有限公司 | Chicory root storage method |
| CN107242290A (en) * | 2017-07-18 | 2017-10-13 | 宁夏中玺枣业股份有限公司 | Lingwu Long Jujube Inappropriate ADH syndrome pit disease forecooling method |
| CN107535483A (en) * | 2017-08-15 | 2018-01-05 | 合肥元政农林生态科技有限公司 | A kind of storage method of hare's-lettuce seed |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109619093A (en) | 2019-04-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Childers et al. | Influence of submersion of the roots on transpiration, apparent photosynthesis, and respiration of young apple trees | |
| CN105248626B (en) | A kind of processing method inhibiting fruit dry plate brown stain | |
| CN107296091A (en) | A kind of method that super low temperature quick frozen reduces Penaeus Vannmei benevolence percentage of water loss | |
| CN102599233A (en) | Quick-frozen daylily making method | |
| CN105494321A (en) | Seed cryopreservation method | |
| WO2021073609A1 (en) | Method for preparing water-cultured ripe tobacco leaves in shading environment | |
| CN109619093B (en) | Low-temperature freezing living body quality guarantee method | |
| KR101044246B1 (en) | Methods of dried persimmons | |
| CN103300141A (en) | Peach low-temperature storage and fresh keeping method and application of gamma-aminobutyric acid to fresh keeping of peaches | |
| CN107549295A (en) | A kind of micro- method for freezing the nearly fresh-keeping fresh and alive plant agricultural products of freezing point thawing technique of storage | |
| CN105379685A (en) | Method for preserving exorista japonica townsend pupae | |
| CN103749649A (en) | Honey peach heat treatment storage and preservation technology | |
| CN101990933A (en) | Hot air and MeJa compounded treatment refreshing method of loquat fruit | |
| CN105831232A (en) | Blanching-hot air-freezing three-stage type gumbo preservation method and application thereof | |
| CN102302050B (en) | Refrigeration fresh-keeping method of peach | |
| Chandrashekar | Physio-Chemical changes during post harvest handling of onion (Allium cepa L.)-A Review | |
| CN204438637U (en) | A kind of fruit and vegetable fresh-keeping and cold storage storehouse | |
| CN210094165U (en) | Super ice temperature fruit vegetables planting device | |
| CN107751354A (en) | A kind of lotus root point freeze-drying method | |
| CN107593689A (en) | A kind of thin shell mountain pecan peach flower powder processing and freezing and storing method | |
| KR102338267B1 (en) | Method for Freezing Storage of Tree Ear and Tree Ear Stored therefrom | |
| CN112930902A (en) | Method for improving grafting survival rate of blueberries by using micro-freezing technology | |
| CN106721949A (en) | It is a kind of to adjust the method that nostoc chill point significantly prolongs storage period | |
| CN103229762A (en) | Leucaena leucocephala seed ultra-dry preservation method | |
| CN107950640A (en) | A kind of lyophilized technique of the fresh green onion of vacuum freeze drying |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |