CN109612988A - A method of test confined space ozone disinfection effect - Google Patents
A method of test confined space ozone disinfection effect Download PDFInfo
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- CN109612988A CN109612988A CN201811604908.8A CN201811604908A CN109612988A CN 109612988 A CN109612988 A CN 109612988A CN 201811604908 A CN201811604908 A CN 201811604908A CN 109612988 A CN109612988 A CN 109612988A
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- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
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Abstract
The present invention relates to a kind of methods for testing confined space ozone disinfection effect, comprising: (1) preparation of absorbing liquid: measuring the liquor kalii iodide that 20mL concentration is 20%, pours into the conical flask of 500mL, add 350mL distilled water, close plug, is made in the same way of 6 parts, spare；(2) sampling and titration；(3) concentration of ozone: CO3=ANa × B × 24000/V0 is calculated as follows.Criterion: after ozone generator is opened 30 minutes, the concentration of ozone must not be lower than 12mg/L.Using the method for the present invention test confined space ozonization effect, it is easy to operate, test quickly, result it is accurate.
Description
Technical field
The present invention relates to field of pharmaceutical technology, in particular to a kind of method for testing ozone disinfection effect.
Background technique
Ozone is a kind of broad-spectrum high efficacy bactericidal agent, has strong sterilizing effect.Molecular structure is not under room temperature, normal pressure
Stablize, voluntarily resolves into oxygen (O quickly2) and single oxygen atom (O), the latter has very strong activity, has extremely strong oxygen to bacterium
Change effect, ozone oxidation have decomposed enzyme necessary to bacterium internal oxidation glucose and have killed it to destroy its cell membrane.
Extra oxygen atom then can voluntarily be reassembled into oxygen molecule (O2), any toxic residua is not present, is pollution-free disinfectant.
The ozone generator that formulating plant uses, ozone generator use oxygen to prepare ozone for the board-like corona discharge method of gas source, it
Have the characteristics that high efficiency, high cleaning and easy to operate.It is installed in air-conditioner set by design requirement, more easily will
Ozone adds in total air supply duct, carries out disinfection to clean area.About the residual quantity of ozone, sharp-nosed people exists
0.01ppm is i.e. discernable, and general people can smell in 0.02ppm, the people of baryodmia in 0.1ppm, when also can obviously feel
It arrives.This concentration is completely harmless to personnel, and makes one feeling of freshness.I.e. up to 0.1ppm, seashore reaches for general forest area
0.05ppm.When summer is sunny, concentration is up to 0.03-0.1ppm in certain cities.What the Ministry of Public Health, China formulated for 1979
Regulation in " industrial hygiene standard ", the safety standard of ozone are 0.15ppm.Unite States Standard regulation, personnel can be in 0.1ppm concentration
Under, it works 8 hours.International ozone association criterion regulation.Allow work under 0.1ppm concentration using the professional indoor of ozone
10 hours.
The sterilizing installation of pharmaceutical production workshop will follow fast sterilizing speed, highly-safe, good energy saving property, performance stabilization, behaviour
Make convenient and fast principle to be selected, the sterilization effect of all sterilizing installations be verified, it is ensured that sterilizing installation has suitability.
The quality that the effect of workshop sterilizing is final with finished product is closely related, it is therefore desirable to test the sterilization effect in workshop
Card.In the prior art, verifying sterilization effect most straightforward approach is the clump count for measuring air, body surface.But the training of bacterium
It supports and needs the time, show that test result generally requires to wait for one to a couple of days, be unfavorable for scheduling of production.
It would therefore be highly desirable to a kind of method for inventing fast verification ozone sterilization effect.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of methods for testing confined space ozone disinfection effect, so that
This method is easy to operate, test is quick, result is accurate.
In order to solve the above-mentioned technical problem, the following technical solutions are proposed by the present invention:
A method of test confined space ozone disinfection effect, which is characterized in that the method is by the following steps group
At:
(1) preparation of absorbing liquid: measuring the liquor kalii iodide that 20mL concentration is 20%, pour into the conical flask of 500mL,
Add 350ml distilled water, close plug is made in the same way of 6 parts, spare；
(2) sampling and titration: 100mL syringe is used at 20,30,40 minutes, to extract rapidly in scheduled room respectively
Gas 1000mL, is slowly injected into absorbing liquid, and 5mL sulfuric acid solution is added immediately and shakes up, stands 5 minutes, uses sodium thiosulfate
The starch solution 0.8-1.2mL of 8-12mg/mL is added in solution titration when solution is in light yellow, continues to be titrated to color disappearance
For it, the dosage of sodium thiosulfate is recorded；
(3) concentration of ozone is calculated as follows:
CO3=ANa × B × 24000/V0, in formula:
CO3-ozone concentration, mg/L；
ANa-sodium thiosulfate titer dosage, mL；
B-sodium thiosulfate concentration of standard solution, mol/L；
V0-ozonated air sample volume, mL；
Criterion: after ozone generator is opened 30 minutes, the concentration of ozone must not be lower than 12mg/L.
It preferably, the position sampled in the step (2) should be far from air outlet.
Preferably, the concentration of starch solution is 12mg/mL in the step (2).
Preferably, the dosage of starch solution is 1.0mL in the step (2).
Using the method for the present invention test confined space ozonization effect, it is easy to operate, test quickly, result it is accurate.
Advantageous effects of the invention can be proved by following tests.
Corresponding relationship between 1 ozone concentration standard of test example and sterilization effect
Test method is tested the concentration of ozone in air by the method for the present invention, while carrying out settling bacteria test and surface sterilization
The corresponding relationship between ozone concentration standard and sterilization effect is verified in test.
After preparation is ready before settling bacteria test method is tested, existed respectively with sterilized Φ 90mm nutrient agar plate
After testing room sedimentation collection bacterium 30min, ozone generator is opened, 30 minutes sterilizing tests is carried out, plate is placed on 37 DEG C of cultures
Culture 48 disappears in case, makees count plate, and (bacterium is compareed in addition to not having to ozone, with experimental group condition phase compared with compareing bacterium
Together), sterilizing rate is calculated.
It is 5 × 5cm that surface sterilization test marks area respectively in test room on away from ground 0.8m eminence color steel plate
Two at be used as test point, with spare after drinking water wiped clean.For sample before taking sterilizing as reference substance, method is with nothing
Bacterium purifies water-moistened cotton swab surface；It learns from else's experience with method the sample after ozonization, control and cotton swab is put into and is filled respectively
Impregnate 10min in 10mL axenic purification water test tube, shake well is with the bacterium washed on lower cotton swab and is uniformly mixed, and precision measures
1mL washing lotion is cultivated 48 hours in 37 DEG C of incubators；Make count plate, and compared with compareing bacterium (bacterium is compareed in addition to not having to ozone,
It is identical as experimental group condition), calculate sterilizing rate.
Test result
Test result see the table below: ozone concentration is tested using the method for the present invention, it is smelly after ozone generator is opened 30 minutes
As long as the concentration of oxygen is more than or equal to 12mg/L, either settling bacteria test or surface sterilization test, sterilizing rate is
100%.The result shows that can directly reflect sterilization effect using test ozone concentration.Therefore the method for the present invention test is quick, ties
Fruit is accurate.
Corresponding relationship between 1 ozone concentration standard of table and sterilization effect
In order to preferably illustrate technical solution, the present invention is further illustrated With reference to embodiment, but
Protection scope of the presently claimed invention is not limited to the following example.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example only a part of the embodiments of the present invention.The scope of protection of present invention is not limited to following embodiments.
Embodiment 1
The method for testing confined space ozone disinfection effect
(1) preparation of absorbing liquid: measuring the liquor kalii iodide that 20mL concentration is 20%, pour into the conical flask of 500mL,
Add 350ml distilled water, close plug is made in the same way of 6 parts, spare；
(2) sampling and titration: use 100mL syringe respectively at 20,30,40 minutes, in scheduled room far from outlet air
Gas 1000mL is extracted rapidly in the position of mouth, is slowly injected into absorbing liquid, and 5mL sulfuric acid solution is added immediately and shakes up, stands 5 points
Clock is titrated with hypo solution, and the starch solution 0.8mL of 8mg/mL is added when solution is in light yellow, continues to be titrated to
Color disappears for it, records the dosage of sodium thiosulfate；
(3) concentration of ozone is calculated as follows:
CO3=ANa × B × 24000/V0, in formula:
CO3-ozone concentration, mg/L；
ANa-sodium thiosulfate titer dosage, mL；
B-sodium thiosulfate concentration of standard solution, mol/L；
V0-ozonated air sample volume, mL；
As a result: after ozone generator is opened 30 minutes, the concentration of ozone is 13.8mg/L.
Beneficial effect
See test example.
Embodiment 2
The method for testing confined space ozone disinfection effect
(1) preparation of absorbing liquid: measuring the liquor kalii iodide that 20mL concentration is 20%, pour into the conical flask of 500mL,
Add 350ml distilled water, close plug is made in the same way of 6 parts, spare；
(2) sampling and titration: use 100mL syringe respectively at 20,30,40 minutes, in scheduled room far from outlet air
Gas 1000mL is extracted rapidly in the position of mouth, is slowly injected into absorbing liquid, and 5mL sulfuric acid solution is added immediately and shakes up, stands 5 points
Clock is titrated with hypo solution, and the starch solution 1.2mL of 10mg/mL is added when solution is in light yellow, continues to be titrated to
Color disappears for it, records the dosage of sodium thiosulfate；
(3) concentration of ozone is calculated as follows:
CO3=ANa × B × 24000/V0, in formula:
CO3-ozone concentration, mg/L；
ANa-sodium thiosulfate titer dosage, mL；
B-sodium thiosulfate concentration of standard solution, mol/L；
V0-ozonated air sample volume, mL；
As a result: after ozone generator is opened 30 minutes, the concentration of ozone is 13.8mg/L.
Beneficial effect
See test example.
Embodiment 3
The method for testing confined space ozone disinfection effect
(1) preparation of absorbing liquid: measuring the liquor kalii iodide that 20mL concentration is 20%, pour into the conical flask of 500mL,
Add 350ml distilled water, close plug is made in the same way of 6 parts, spare；
(2) sampling and titration: use 100mL syringe respectively at 20,30,40 minutes, in scheduled room far from outlet air
Gas 1000mL is extracted rapidly in the position of mouth, is slowly injected into absorbing liquid, and 5mL sulfuric acid solution is added immediately and shakes up, stands 5 points
Clock is titrated with hypo solution, and the starch solution 1.0mL of 12mg/mL is added when solution is in light yellow, continues to be titrated to
Color disappears for it, records the dosage of sodium thiosulfate；
(3) concentration of ozone is calculated as follows:
CO3=ANa × B × 24000/V0, in formula:
CO3-ozone concentration, mg/L；
ANa-sodium thiosulfate titer dosage, mL；
B-sodium thiosulfate concentration of standard solution, mol/L；
V0-ozonated air sample volume, mL；
As a result: after ozone generator is opened 30 minutes, the concentration of ozone is 13.8mg/L.
Beneficial effect
See test example.
Obviously, the above embodiment is merely an example for clearly illustrating the present invention, and is not to of the invention real
Apply the restriction of mode.For those of ordinary skill in the art, it can also make on the basis of the above description other
Various forms of variations or variation.There is no necessity and possibility to exhaust all the enbodiments.It is all in spirit of the invention
With any modifications, equivalent replacements, and improvements made within principle etc., the protection scope of the claims in the present invention should be included in
Within.
Claims (4)
1. a kind of method for testing confined space ozone disinfection effect, which is characterized in that the method is made of the following steps:
(1) preparation of absorbing liquid: measuring the liquor kalii iodide that 20mL concentration is 20%, pour into the conical flask of 500mL, then plus
Enter 350mL distilled water, close plug is made in the same way of 6 parts, spare；
(2) sampling and titration: 100mL syringe is used at 20,30,40 minutes, to extract gas rapidly in scheduled room respectively
1000mL is slowly injected into absorbing liquid, and 5mL sulfuric acid solution is added immediately and shakes up, stands 5 minutes, uses hypo solution
The starch solution 0.8-1.2mL of 8-12mg/mL is added in titration when solution is in light yellow, continues to be titrated to color disappearance for it,
Record the dosage of sodium thiosulfate；
(3) concentration of ozone is calculated as follows:
CO3=ANa × B × 24000/V0, in formula:
CO3-ozone concentration, mg/L；
ANa-sodium thiosulfate titer dosage, mL；
B-sodium thiosulfate concentration of standard solution, mol/L；
V0-ozonated air sample volume, mL；
Criterion: after ozone generator is opened 30 minutes, the concentration of ozone must not be lower than 12mg/L.
2. a kind of method for testing confined space ozone disinfection effect according to claim 1, which is characterized in that the step (2)
It the position of middle sampling should be far from air outlet.
3. according to a kind of method for testing confined space ozone disinfection effect of claim 2, which is characterized in that the step (2)
The concentration of middle starch solution is 12mg/mL.
4. according to a kind of method for testing confined space ozone disinfection effect of claim 3, which is characterized in that in the step (2)
The dosage of starch solution is 1.0mL.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811604908.8A CN109612988A (en) | 2018-12-26 | 2018-12-26 | A method of test confined space ozone disinfection effect |
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| CN201811604908.8A CN109612988A (en) | 2018-12-26 | 2018-12-26 | A method of test confined space ozone disinfection effect |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111521605A (en) * | 2020-04-30 | 2020-08-11 | 上海尚析环保设备有限公司 | Simple device and method for measuring gas-phase ozone concentration |
-
2018
- 2018-12-26 CN CN201811604908.8A patent/CN109612988A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111521605A (en) * | 2020-04-30 | 2020-08-11 | 上海尚析环保设备有限公司 | Simple device and method for measuring gas-phase ozone concentration |
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Application publication date: 20190412 |