CN109609569A - A kind of sweet potato fructose syrup preparation method - Google Patents
A kind of sweet potato fructose syrup preparation method Download PDFInfo
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- CN109609569A CN109609569A CN201910009833.7A CN201910009833A CN109609569A CN 109609569 A CN109609569 A CN 109609569A CN 201910009833 A CN201910009833 A CN 201910009833A CN 109609569 A CN109609569 A CN 109609569A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
Abstract
The present invention provides a kind of sweet potato fructose syrup preparation method, belongs to fructose syrup preparation technical field.This method includes that combined-enzyme method sweet potato glucose syrup preparation process, glucose isomer enzyme tautomerize to the techniques such as fructose technique, syrup decolorization technique, syrup elution concentration.This rational technology is feasible, with low investment, technological operation is simple, practical, high production efficiency, can produce beneficial to consumer's health, flavor taste is all good, can low tempertaure storage high-quality sweet potato fructose syrup, extend Sweet Potato Industry chain with this, develops our province with the production technology of sweet potato production fructose syrup.
Description
Technical field
The invention belongs to fructose syrup preparation technical fields, and in particular to a kind of sweet potato fructose syrup preparation method.
Background technique
Fructose syrup also known as high fructose syrup, isomery syrup, it is to pass through grape with the enzyme process effect resulting saccharified liquid of starch
The isomerization of sugared isomerase, the mixing molasses that a part of glucose isomerase is formed for fructose are one kind with fructose and Portugal
Grape sugar is the health novel starch sugar product of main sugar.
Fructose syrup is since the 1970s realizes industrialized production, due to its production is not limited by region and season
It rapidly develops；In recent years, in the environment that energy shortage, international and domestic sugared valence remain high, it is widely used in food row
Industry, especially soft-drink industry.
Compared with sucrose, fructose syrup is with high sugariness, unique flavor, high-dissolvability, more cold more sweet tea, low in calories, osmotic pressure
The characteristics such as big, occupy world's soft drink industry first, gradually replace sucrose role, effectively reduce cost, thus solve sugarcane
The sugared insufficient Industry of supply.The use of fructose syrup will not only destroy food mouthfeel, moreover it is possible to increase mellow flavor,
It ensure that the genuineness of food；Meanwhile it is as a kind of comparatively ideal novel sweetener, to the elderly and diabetic
Speech, blood sugar concentration variation are unobvious；As a kind of good nutrition agent, human body internal protein can be inhibited to consume, in coronary heart disease, painstaking effort
There is good therapeutic effect in some diseases such as pipe disease, the loss of human body protein can be supplemented.Therefore, fructose syrup is due to it
Application is wide, excellent, and effect performance multiplicity is worldwide increasingly deep generally to be approved by people, is increasingly becoming sugarcane
Third class sugar source other than sugar and beet sugar.
In recent years, the production of fructose syrup mostly with cornstarch, crack rice, wheat Crude starch etc. is raw material, and be with sweet potato
The research report of raw material is less.Sweet potato is as the second largest cereal crops in Fujian Province, and cultivated area is big, yield is more, but mainly quilt
To extract starch and be processed into the primary processed items such as candied fruit, tasty and crisp flake, vermicelli, product is single, and industrial chain is short, institute
Take benefit lower.
The further investigation with people to fructose syrup superiority, fructose syrup become more next in its application field simultaneously
Wider, market demand is also increasing, also increases therewith to its quality requirement.Due to the height of fructose syrup social status
The shortcomings that depending primarily on the fructose content in its mixing molasses, and F42 sugar is because its sugariness is close with sucrose, showing is Portugal
Grape sugared content is higher, and crystallization is easy when low temperature, it has not been convenient to store, be insufficient for the demand of the industries such as medical and health care.
It is domestic at present to solve that fructose content in fructose syrup is low, easy crystallization is not easy the method stored and mainly prepares high fructose
The fructose syrup of content, i.e. F55 fructose syrup.But there is complex technical process, production efficiency in preparation F55 fructose syrup mostly
Low, the problems such as cost of investment is high.
Therefore it develops that a kind of reasonable, with low investment, technological operation is simple, practical, high production efficiency, can produce
Beneficial to consumer's health, flavor taste it is all good, can low tempertaure storage high-quality fructose syrup, and meet market industry demand
Using sweet potato as the fructose syrup preparation method of raw material, to promote the progress of fructose syrup technology of preparing play an important role, together
When extensible Sweet Potato Industry chain, our province is developed with the production technology of sweet potato production fructose syrup with this.
Summary of the invention
The purpose of the present invention is to provide a kind of sweet potato fructose syrup preparation methods, overcome existing fructose syrup preparation production
Present in complex technical process, production efficiency is low, the storage of cost of investment high and low temperature easily crystallization the problems such as.The present invention is with sweet potato
For raw material, fructose syrup is prepared using combined-enzyme method and glucose isomer enzyme tautomerizes to the technologies Integrated Development such as fructose
A kind of sweet potato fructose syrup preparation method.
A kind of sweet potato fructose syrup preparation method, comprising the following steps: the preparation of combined-enzyme method sweet potato glucose syrup, glucose
Allomerase tautomerizes to fructose, mixing preparation.
Combined-enzyme method sweet potato glucose syrup preparation the following steps are included:
(1) the commercially available fresh sweet potato of selection, removal of impurities are cleaned, after peeling slice, and being put into food steamer expires 10~15 min of steaming after gas, then will
The sweet potato cooked is beaten in beater after mixing in proportion with water to homogenate；
(2) it adjusts sweet potato to be homogenized to pH=6.0 ± 0.5, adds to heat after anhydrous calcium chloride being gelatinized；It is risen to homogenized temperature
90 DEG C ± 2 DEG C, mesophilicα-diastase, 40~60 min of liquefaction reaction, through high temperature after liquid glucose DE value is up to 15%~20% is added
Enzyme deactivation is centrifuged to obtain supernatant；
(3) gained supernatant is cooled to room temperature, is adjusted to pH=4.0~4.5, carbohydrase is added, is saccharified at 60 DEG C ± 2 DEG C
24~48 h are reacted, high temperature enzyme deactivation obtains saccharified liquid after liquid glucose DE value is up to 95% or more；
(4) it decolourizes: gained saccharified liquid being subjected to active carbon decoloring, diatomite is filtered by vacuum；
(5) saccharified liquid after decoloration elution concentration: is obtained into solid content through ion exchange desalination bleaching, vacuum evaporation process
Content is that 45%~50% high-purity refines sweet potato glucose syrup.
The glucose isomer enzyme tautomerize to fructose the following steps are included:
(1) take solid content be 45%~50% high-purity refine sweet potato glucose syrup, be added epsom salt, cobalt chloride with
And sodium pyrosulfite；PH=7.5~8.0 are adjusted later, and glucose isomerase, the isomerization reaction 25 at 60 DEG C ± 2 DEG C is added
~30 h survey fructose content after high temperature enzyme deactivation；
(2) it decolourizes: gained liquid glucose being subjected to active carbon decoloring, diatomite is filtered by vacuum；
(3) liquid glucose after decoloration elution concentration: is obtained into F42 fruit Portugal through ion exchange desalination bleaching, vacuum evaporation process
Syrup.
The mixing preparation specifically includes following steps, by the F42 fructose syrup being prepared after chromatographic isolation, carries out
It blends, then is concentrated to get the fructose syrup of F55 through eluting.
The sweet potato mixes with water 1:2 in mass ratio, Ca after the anhydrous calcium chloride is added2+Final concentration of 150 ppm,
The additional amount of the mesophilicα-diastase is 20 U/g substrates, and the carbohydrase additional amount is 140 U/g substrates.
In the high-purity purification sweet potato glucose syrup, final concentration of 45 mg/kg of epsom salt, cobalt chloride final concentration
For 0.001 mol/L, final concentration of 100 ppm of sodium pyrosulfite；The glucose isomerase additional amount is 12 mg/g substrates.
The syrup decolorization technique the following steps are included:
To be cooled to decolouring liquid glucose 50 DEG C hereinafter, adjust pH=4.0~4.5, be added 303 sugared Powdered Activated Carbons, 50 DEG C ±
2 DEG C of 30 min of intermittent stirring carry out adsorption bleaching to liquid glucose；After decoloration, it is true that diatomite is carried out in conjunction with super-cell
Empty pump filters out charcoal；
Specific process is filtered by vacuum in the diatomite are as follows: a filter paper is put in Buchner funnel, the sugar diatomite that will be modulated
Equably layer overlay, then it is poured slowly into liquid glucose, carry out suction filtration removal of impurities.
Syrup elution concentration technology the following steps are included:
Ion exchange desalination bleaching: suitable resin cation and resin anion (R.A.) are selected；Liquid glucose is flowed through with the flow velocity of 1.5 L/h
Sun-male-female-anion-exchange column collects liquid glucose until detection liquid glucose conductivity is less than 20 μ S/cm；
Vacuum evaporation: by the liquid glucose after desalination of decolourizing in carrying out vacuum evaporation, being concentrated into required solid content,
And make light transmittance up to 90% or more.
303 sugar is 3wt% with Powdered Activated Carbon additional amount；The sugar diatomite modulator approach are as follows: in mass ratio
Thick type diatomite and thin type diatomite is added in 1:1, and 3wt% distilled water is added to stir and evenly mix.
The resin cation is 732 cation exchange resins, D101 macroporous absorbent resin, DM11 macroporous absorbent resin three
Kind resin 1:1:1 ratio in mass ratio mixes；The resin anion (R.A.) is 201X7 strong-basicity styrene series anion exchange tree
Rouge.
The process flow are as follows:
(1) fresh sweet potato → removal of impurities is cleaned, and → peeling, which is sliced, → to be cooked and → deploys and → be beaten → gelatinization → to liquefy (medium temperature α-shallow lake
Powder enzyme) → high temperature enzyme deactivation → saccharification (carbohydrase) → high temperature enzyme deactivation → saccharified liquid → activity carbon decoloring → diatomite vacuum filtration →
Ion exchange → vacuum evaporation → purification sweet potato glucose syrup.
(2) sweet potato glucose syrup → isomerization (glucose isomerase) → high temperature enzyme deactivation → activity carbon decoloring → diatom is refined
Native vacuum filtration → ion exchange → vacuum evaporation → F42 fructose syrup.
(3) F42 fructose syrup → chromatographic isolation → F90 fructose syrup → mixing preparation (adding F42 fructose syrup) → ion
Exchange → vacuum evaporation → F55 sweet potato fructose syrup.
The present invention has the advantages that
(1) preparation method reasonable of the present invention, with low investment, technological operation is simple, practical, high production efficiency, can produce
Out beneficial to consumer's health, flavor taste it is all good, can low tempertaure storage high-quality fructose syrup, and meet market industry need
Ask using sweet potato as the fructose syrup preparation method of raw material, to promote the progress of fructose syrup technology of preparing play an important role.
(2) in the prior art the production of fructose syrup mostly with cornstarch, crack rice, wheat Crude starch etc. is raw material, and with
Sweet potato is that the research report of raw material is less；Sweet potato is as the second largest cereal crops in Fujian Province, and cultivated area is big, yield is more, this hair
It is bright to can reach the purpose for extending Sweet Potato Industry chain, our province is developed with the production technology of sweet potato production fructose syrup with this.
(3) F42 sugar because its sugariness it is close with sucrose, the shortcomings that showing, is higher for glucose content, and holds when low temperature
Easily crystallization, it has not been convenient to store, F55 sweet potato fructose syrup low-temperature storage number of days prepared by the present invention can reach 100 days, storage
Number of days extends 70 days than commercially available general F55 fructose syrup.
Specific embodiment
In order to make content of the present invention easily facilitate understanding, With reference to embodiment to of the present invention
Technical solution is described further, but the present invention is not limited only to this.
Embodiment 1
(1) combined-enzyme method sweet potato glucose syrup preparation process:
Select commercially available fresh sweet potato, removal of impurities is cleaned, after peeling slice, is put into food steamer and is expired and steam 13 min after gas, then will cook
Sweet potato and water are beaten in the ratio of 1:2 in beater to homogenate；
It adjusts sweet potato to be homogenized to pH=6.0, adding anhydrous calcium chloride makes Ca in slurry2+Concentration is pasted up to 150 ppm, rear heating
Change；90 DEG C are risen to homogenized temperature, 20 U/g mesophilicα-diastases are added by substrate quality, 50 min of liquefaction reaction works as liquid glucose
DE value up to after 18% through high temperature enzyme deactivation, be centrifuged to obtain supernatant；
Gained supernatant is cooled to room temperature, pH=4.3 are adjusted to, and 140 U/g carbohydrase are added by substrate quality, at 60 DEG C
Saccharification 36 h of reaction, high temperature enzyme deactivation obtains saccharified liquid (i.e. the sweet potato Glucose Liquid of no bleaching) after liquid glucose DE value is up to 98%；
Above-mentioned gained is cooled to 50 DEG C hereinafter, adjusting pH=4.3, are added 303 sugar powder activity of 3wt% to decolouring liquid glucose
Charcoal, 50 DEG C of 30 min of intermittent stirring carry out adsorption bleaching to liquid glucose.After decoloration, vacuum is carried out in conjunction with super-cell
Filter carbon removal.Wherein specific process is filtered by vacuum in diatomite are as follows: a filter paper is put in Buchner funnel, the sugar silicon that will be modulated
Diatomaceous earth equably layer overlay, then it is poured slowly into liquid glucose, carry out suction filtration removal of impurities.Wherein sugar diatomite modulator approach are as follows: addition
Ratio is the thick type diatomite and thin type diatomite of 1:1, and 3wt% distilled water is added to stir and evenly mix.
Ion exchange desalination bleaching: resin cation select 732 cation exchange resins, D101 macroporous absorbent resin,
Three kinds of resins of DM11 macroporous absorbent resin are used in mixed way in 1:1:1 ratio, and resin anion (R.A.) uses 201X7 strong-basicity styrene series
Anion exchange resin.Liquid glucose flows through sun-male-female-anion-exchange column with the flow velocity of 1.5 L/h (i.e. 10 rpm), until detection
Until liquid glucose conductivity < 20 μ S/cm, liquid glucose is collected.
Vacuum evaporation: by the liquid glucose after desalination of decolourizing in carrying out vacuum evaporation, being concentrated into solid content is
48%, and make light transmittance up to 90% or more, that is, high-purity purification sweet potato glucose syrup is prepared.(2) glucose isomer enzyme
Tautomerize to fructose technique:
Above-mentioned high-purity purification sweet potato glucose syrup (solid content 48%) is taken, 45 mg/kg epsom salts, chlorination are added
Cobalt and sodium pyrosulfite control Mg in syrup2+Concentration is up to 45 mg/kg, Co2+Concentration is up to 0.001 mol/L, Ca2+Concentration 0~
2 mg/kg, SO2Concentration is up to 100 ppm.PH=7.8 are adjusted later, by the glucose isomerase that 12 mg/g substrates are added, in 60
28 h of isomerization reaction at DEG C is surveyed fructose content (at this time after isomerization gained liquid glucose without decolorization) after high temperature enzyme deactivation；
Above-mentioned gained is cooled to 50 DEG C hereinafter, adjusting pH=4.3, are added 303 sugar powder activity of 3wt% to decolouring liquid glucose
Charcoal, 50 DEG C of 30 min of intermittent stirring carry out adsorption bleaching to liquid glucose.After decoloration, vacuum is carried out in conjunction with super-cell
Filter carbon removal.Wherein specific process is filtered by vacuum in diatomite are as follows: a filter paper is put in Buchner funnel, the sugar silicon that will be modulated
Diatomaceous earth equably layer overlay, then it is poured slowly into liquid glucose, carry out suction filtration removal of impurities.Wherein sugar diatomite modulator approach are as follows: addition
Ratio is the thick type diatomite and thin type diatomite of 1:1, and 3wt% distilled water is added to stir and evenly mix.
Ion exchange desalination bleaching: resin cation select 732 cation exchange resins, D101 macroporous absorbent resin,
Three kinds of resins of DM11 macroporous absorbent resin are used in mixed way in 1:1:1 ratio, and resin anion (R.A.) uses 201X7 strong-basicity styrene series
Anion exchange resin.Liquid glucose flows through sun-male-female-anion-exchange column with the flow velocity of 1.5 L/h (i.e. 10 rpm), until detection
Until liquid glucose conductivity < 20 μ S/cm, liquid glucose is collected.
Vacuum evaporation: by the liquid glucose after desalination of decolourizing in carrying out vacuum evaporation, being concentrated into solid content is
71% F42 fructose syrup (fructose content 45%), and make light transmittance up to 90% or more.
(3) (732 cation exchange resins and DM11 are big through chromatographic isolation for the F42 fructose syrup that step (2) is prepared
Macroporous adsorbent resin is used in mixed way in the ratio of 1:1) obtain F90 fructose syrup (fructose content 87%), after will be prepared
F42 fructose syrup and the F90 fructose syrup ratio of 25:9 in mass ratio blent, desalination, recycle ion exchange resin into
Row decoloration desalination is concentrated by evaporation；
Ion exchange desalination bleaching: resin cation selects 732 cation exchange resins, D101 macroporous absorbent resin, DM11 big
Three kinds of resins of macroporous adsorbent resin are used in mixed way in 1:1:1 ratio, and resin anion (R.A.) uses 201X7 strong-basicity styrene series anion
Exchanger resin.Liquid glucose flows through sun-male-female-anion-exchange column with the flow velocity of 1.5 L/h (i.e. 10 rpm), until detection liquid glucose electricity
Until conductance < 20 μ S/cm, liquid glucose is collected.
Vacuum evaporation: by the liquid glucose after desalination of decolourizing in carrying out vacuum evaporation, it is concentrated to get solid content
For the fructose syrup (fructose content 60%) of 77% F55.
Embodiment 2
(1) combined-enzyme method sweet potato glucose syrup preparation process:
Select commercially available fresh sweet potato, removal of impurities is cleaned, after peeling slice, is put into food steamer and is expired and steam 10 min after gas, then will cook
Sweet potato and water are beaten in the ratio of 1:2 in beater to homogenate；
It adjusts sweet potato to be homogenized to pH=5.5, adding anhydrous calcium chloride makes Ca in slurry2+Concentration is pasted up to 150 ppm, rear heating
Change；88 DEG C are risen to homogenized temperature, 20 U/g mesophilicα-diastases are added by substrate quality, 40 min of liquefaction reaction works as liquid glucose
DE value up to after 15% through high temperature enzyme deactivation, be centrifuged to obtain supernatant；
Gained supernatant is cooled to room temperature, pH=4.0 are adjusted to, and 140 U/g carbohydrase are added by substrate quality, at 58 DEG C
Saccharification 24 h of reaction, high temperature enzyme deactivation obtains saccharified liquid (i.e. the sweet potato Glucose Liquid of no bleaching) after liquid glucose DE value is up to 95%；
Above-mentioned gained is cooled to 50 DEG C hereinafter, adjusting pH=4.0, are added 303 sugar powder activity of 3wt% to decolouring liquid glucose
Charcoal, 48 DEG C of ± 2 DEG C of 30 min of intermittent stirring carry out adsorption bleaching to liquid glucose.After decoloration, in conjunction with super-cell into
Row vacuum filtration carbon removal.Wherein specific process is filtered by vacuum in diatomite are as follows: a filter paper is put in Buchner funnel, by what is modulated
Sugar diatomite equably layer overlay, then it is poured slowly into liquid glucose, carry out suction filtration removal of impurities.Wherein sugar diatomite modulator approach
Are as follows: adding proportion is the thick type diatomite and thin type diatomite of 1:1, and 3wt% distilled water is added to stir and evenly mix.
Ion exchange desalination bleaching: resin cation select 732 cation exchange resins, D101 macroporous absorbent resin,
Three kinds of resins of DM11 macroporous absorbent resin are used in mixed way in 1:1:1 ratio, and resin anion (R.A.) uses 201X7 strong-basicity styrene series
Anion exchange resin.Liquid glucose flows through sun-male-female-anion-exchange column with the flow velocity of 1.5 L/h (i.e. 10 rpm), until detection
Until liquid glucose conductivity < 20 μ S/cm, liquid glucose is collected.
Vacuum evaporation: by the liquid glucose after desalination of decolourizing in carrying out vacuum evaporation, being concentrated into solid content is
45%, and make light transmittance up to 90% or more, that is, high-purity purification sweet potato glucose syrup is prepared.
(2) glucose isomer enzyme tautomerizes to fructose technique:
Above-mentioned high-purity purification sweet potato glucose syrup (solid content 45%) is taken, 45 mg/kg epsom salts, chlorination are added
Cobalt and sodium pyrosulfite control Mg in syrup2+Concentration is up to 45 mg/kg, Co2+Concentration is up to 0.001 mol/L, Ca2+Concentration 0~
2 mg/kg, SO2Concentration is up to 100 ppm.PH=7.5 are adjusted later, by the glucose isomerase that 12 mg/g substrates are added, in 58
25 h of isomerization reaction at DEG C is surveyed fructose content (at this time after isomerization gained liquid glucose without decolorization) after high temperature enzyme deactivation；
Above-mentioned gained is cooled to 50 DEG C hereinafter, adjusting pH=4.0, are added 303 sugar powder activity of 3wt% to decolouring liquid glucose
Charcoal, 48 DEG C of ± 2 DEG C of 30 min of intermittent stirring carry out adsorption bleaching to liquid glucose.After decoloration, in conjunction with super-cell into
Row vacuum filtration carbon removal.Wherein specific process is filtered by vacuum in diatomite are as follows: a filter paper is put in Buchner funnel, by what is modulated
Sugar diatomite equably layer overlay, then it is poured slowly into liquid glucose, carry out suction filtration removal of impurities.Wherein sugar diatomite modulator approach
Are as follows: adding proportion is the thick type diatomite and thin type diatomite of 1:1, and 3wt% distilled water is added to stir and evenly mix.
Ion exchange desalination bleaching: resin cation select 732 cation exchange resins, D101 macroporous absorbent resin,
Three kinds of resins of DM11 macroporous absorbent resin are used in mixed way in 1:1:1 ratio, and resin anion (R.A.) uses 201X7 strong-basicity styrene series
Anion exchange resin.Liquid glucose flows through sun-male-female-anion-exchange column with the flow velocity of 1.5 L/h (i.e. 10 rpm), until detection
Until liquid glucose conductivity < 20 μ S/cm, liquid glucose is collected.
Vacuum evaporation: by the liquid glucose after desalination of decolourizing in carrying out vacuum evaporation, it is concentrated to get solid content
For 69% F42 fructose syrup (fructose content 43%), and make light transmittance up to 90% or more.(3) step (2) is prepared
F42 fructose syrup through chromatographic isolation, (732 cation exchange resins and DM11 macroporous absorbent resin are made in the ratio mixing of 1:1
With) obtain F90 fructose syrup (fructose content 85%), after by the F42 fructose syrup being prepared and F90 fructose syrup by
The ratio of mass ratio 25:9 blent, desalination, and ion exchange resin is recycled to carry out decoloration desalination, be concentrated by evaporation；
Ion exchange desalination bleaching: resin cation selects 732 cation exchange resins, D101 macroporous absorbent resin, DM11 big
Three kinds of resins of macroporous adsorbent resin are used in mixed way in 1:1:1 ratio, and resin anion (R.A.) uses 201X7 strong-basicity styrene series anion
Exchanger resin.Liquid glucose flows through sun-male-female-anion-exchange column with the flow velocity of 1.5 L/h (i.e. 10 rpm), until detection liquid glucose electricity
Until conductance < 20 μ S/cm, liquid glucose is collected.
Vacuum evaporation: by the liquid glucose after desalination of decolourizing in carrying out vacuum evaporation, obtaining solid content is 75%
F55 fructose syrup (fructose content 58%).
Embodiment 3
(1) combined-enzyme method sweet potato glucose syrup preparation process:
Select commercially available fresh sweet potato, removal of impurities is cleaned, after peeling slice, is put into food steamer and is expired and steam 15 min after gas, then will cook
Sweet potato and water are beaten in the ratio of 1:2 in beater to homogenate；
It adjusts sweet potato to be homogenized to pH=6.5, adding anhydrous calcium chloride makes Ca in slurry2+Concentration is pasted up to 150 ppm, rear heating
Change；92 DEG C are risen to homogenized temperature, 20 U/g mesophilicα-diastases are added by substrate quality, 60 min of liquefaction reaction works as liquid glucose
DE value up to after 20% through high temperature enzyme deactivation, be centrifuged to obtain supernatant；
Gained supernatant is cooled to room temperature, pH=4.5 are adjusted to, and 140 U/g carbohydrase are added by substrate quality, at 62 DEG C
Saccharification 48 h of reaction, high temperature enzyme deactivation obtains saccharified liquid (i.e. the sweet potato Glucose Liquid of no bleaching) after liquid glucose DE value is up to 96%；
Above-mentioned gained is cooled to 50 DEG C hereinafter, adjusting pH=4.5, are added 303 sugar powder activity of 3wt% to decolouring liquid glucose
Charcoal, 52 DEG C of 30 min of intermittent stirring carry out adsorption bleaching to liquid glucose.After decoloration, vacuum is carried out in conjunction with super-cell
Filter carbon removal.Wherein specific process is filtered by vacuum in diatomite are as follows: a filter paper is put in Buchner funnel, the sugar silicon that will be modulated
Diatomaceous earth equably layer overlay, then it is poured slowly into liquid glucose, carry out suction filtration removal of impurities.Wherein sugar diatomite modulator approach are as follows: addition
Ratio is the thick type diatomite and thin type diatomite of 1:1, and 3wt% distilled water is added to stir and evenly mix.
Ion exchange desalination bleaching: resin cation select 732 cation exchange resins, D101 macroporous absorbent resin,
Three kinds of resins of DM11 macroporous absorbent resin are used in mixed way in 1:1:1 ratio, and resin anion (R.A.) uses 201X7 strong-basicity styrene series
Anion exchange resin.Liquid glucose flows through sun-male-female-anion-exchange column with the flow velocity of 1.5 L/h (i.e. 10 rpm), until detection
Until liquid glucose conductivity < 20 μ S/cm, liquid glucose is collected.
Vacuum evaporation: by the liquid glucose after desalination of decolourizing in carrying out vacuum evaporation, being concentrated into solid content is
46% high-purity refines sweet potato glucose syrup, and makes light transmittance up to 90% or more.
(2) glucose isomer enzyme tautomerizes to fructose technique:
Above-mentioned high-purity purification sweet potato glucose syrup (solid content 46%) is taken, 45 mg/kg epsom salts, chlorination are added
Cobalt and sodium pyrosulfite control Mg in syrup2+Concentration is up to 45 mg/kg, Co2+Concentration is up to 0.001 mol/L, Ca2+Concentration 0~
2 mg/kg, SO2Concentration is up to 100 ppm.PH=8.0 are adjusted later, and 12 mg/g glucose isomerases are added by substrate quality, in
30 h of isomerization reaction at 62 DEG C is surveyed fructose content (at this time after isomerization gained liquid glucose without decolorization) after high temperature enzyme deactivation；
Above-mentioned gained is cooled to 50 DEG C hereinafter, adjusting pH=4.5, are added 303 sugar powder activity of 3wt% to decolouring liquid glucose
Charcoal, 52 DEG C of 30 min of intermittent stirring carry out adsorption bleaching to liquid glucose.After decoloration, vacuum is carried out in conjunction with super-cell
Filter carbon removal.Wherein specific process is filtered by vacuum in diatomite are as follows: a filter paper is put in Buchner funnel, the sugar silicon that will be modulated
Diatomaceous earth equably layer overlay, then it is poured slowly into liquid glucose, carry out suction filtration removal of impurities.Wherein sugar diatomite modulator approach are as follows: addition
Ratio is the thick type diatomite and thin type diatomite of 1:1, and 3wt% distilled water is added to stir and evenly mix.
Ion exchange desalination bleaching: resin cation select 732 cation exchange resins, D101 macroporous absorbent resin,
Three kinds of resins of DM11 macroporous absorbent resin are used in mixed way in 1:1:1 ratio, and resin anion (R.A.) uses 201X7 strong-basicity styrene series
Anion exchange resin.Liquid glucose flows through sun-male-female-anion-exchange column with the flow velocity of 1.5 L/h (i.e. 10 rpm), until detection
Until liquid glucose conductivity < 20 μ S/cm, liquid glucose is collected.
Vacuum evaporation: by the liquid glucose after desalination of decolourizing in carrying out vacuum evaporation, it is concentrated to get solid content
For 70% F42 fructose syrup (fructose content 44%), and make light transmittance up to 90% or more.
(3) (732 cation exchange resins and DM11 are big through chromatographic isolation for the F42 fructose syrup that step (2) is prepared
Macroporous adsorbent resin is used in mixed way in the ratio of 1:1) obtain F90 fructose syrup (fructose content 86%), after will be prepared
F42 fructose syrup and the F90 fructose syrup ratio of 25:9 in mass ratio blent, desalination, recycle ion exchange resin into
Row decoloration desalination is concentrated by evaporation；
Ion exchange desalination bleaching: resin cation selects 732 cation exchange resins, D101 macroporous absorbent resin, DM11 big
Three kinds of resins of macroporous adsorbent resin are used in mixed way in 1:1:1 ratio, and resin anion (R.A.) uses 201X7 strong-basicity styrene series anion
Exchanger resin.Liquid glucose flows through sun-male-female-anion-exchange column with the flow velocity of 1.5 L/h (i.e. 10 rpm), until detection liquid glucose electricity
Until conductance < 20 μ S/cm, liquid glucose is collected.
Vacuum evaporation: by the liquid glucose after desalination of decolourizing in carrying out vacuum evaporation, obtaining solid content is 76%
F55 fructose syrup (fructose content 59%), and make light transmittance up to 90% or more.
Comparative example 1
In commercially available general F55 fructose syrup fructose content be 55% or so, 0~10 DEG C at a temperature of its storage 30 days after just go out
It now crystallizes, seriously affects consumer's use.
The comparison is as follows:
From above table it can be seen that passing through the obtained F55 sweet potato fructose syrup of technique of embodiment 1, fructose content is
60%, just there is crystalline polamer in product after 0~10 DEG C of 100 d of low tempertaure storage；Pass through the obtained F55 of the technique of embodiment 2
Sweet potato fructose syrup, fructose content 58%, just there is crystalline polamer in product after 0~10 DEG C of 93 d of low tempertaure storage；Pass through
The obtained F55 sweet potato fructose syrup of the technique of embodiment 3, fructose content 59%, after 0~10 DEG C of low tempertaure storage 95d
Just there is crystalline polamer in product；And the fructose content in comparative example 1 in commercially available F55 fructose syrup product is 55%, in 0~10 DEG C
Just has there is crystalline polamer in product after 30 d of low tempertaure storage, seriously affects consumer's use；It is therefore seen that using this technology method
F55 fructose syrup its fructose content height produced, can extend the low tempertaure storage period, be beneficial to consumer's use.
Claims (10)
1. a kind of sweet potato fructose syrup preparation method, which comprises the following steps: combined-enzyme method sweet potato glucose syrup system
Standby, glucose isomer enzyme tautomerizes to fructose, mixing preparation.
2. a kind of sweet potato fructose syrup preparation method according to claim 1, which is characterized in that the combined-enzyme method sweet potato
Glucose syrup preparation the following steps are included:
(1) the commercially available fresh sweet potato of selection, removal of impurities are cleaned, after peeling slice, and being put into food steamer expires 10~15 min of steaming after gas, then will
The sweet potato cooked is beaten in beater after mixing in proportion with water to homogenate；
(2) it adjusts sweet potato to be homogenized to pH=6.0 ± 0.5, adds to heat after anhydrous calcium chloride being gelatinized；It is risen to homogenized temperature
90 DEG C ± 2 DEG C, mesophilicα-diastase, 40~60 min of liquefaction reaction, through high temperature after liquid glucose DE value is up to 15%~20% is added
Enzyme deactivation is centrifuged to obtain supernatant；
(3) gained supernatant is cooled to room temperature, is adjusted to pH=4.0~4.5, carbohydrase is added, is saccharified at 60 DEG C ± 2 DEG C
24~48 h are reacted, high temperature enzyme deactivation obtains saccharified liquid after liquid glucose DE value is up to 95% or more；
(4) it decolourizes: gained saccharified liquid being subjected to active carbon decoloring, diatomite is filtered by vacuum；
(5) saccharified liquid after decoloration elution concentration: is obtained into solid content through ion exchange desalination bleaching, vacuum evaporation process
Content is that 45%~50% high-purity refines sweet potato glucose syrup.
3. a kind of sweet potato fructose syrup preparation method according to claim 1, which is characterized in that the glucose isomerization
Enzyme tautomerize to fructose the following steps are included:
(1) take solid content be 45%~50% high-purity refine sweet potato glucose syrup, be added epsom salt, cobalt chloride with
And sodium pyrosulfite；PH=7.5~8.0 are adjusted later, and glucose isomerase, the isomerization reaction 25 at 60 DEG C ± 2 DEG C is added
~30 h survey fructose content after high temperature enzyme deactivation；
(2) it decolourizes: gained liquid glucose being subjected to active carbon decoloring, diatomite is filtered by vacuum；
(3) liquid glucose after decoloration elution concentration: is obtained into F42 fruit Portugal through ion exchange desalination bleaching, vacuum evaporation process
Syrup.
4. a kind of sweet potato fructose syrup preparation method according to claim 1, which is characterized in that the mixing preparation is specific
Include the following steps, by the F42 fructose syrup being prepared after chromatographic isolation, blent, then is concentrated to get F55 through elution
Fructose syrup.
5. a kind of sweet potato fructose syrup preparation method according to claim 2, which is characterized in that the sweet potato and water press quality
It is mixed than 1:2, Ca after the anhydrous calcium chloride is added2+Final concentration of 150 ppm, the additional amount of the mesophilicα-diastase is
20 U/g substrates, the carbohydrase additional amount are 140 U/g substrates.
6. a kind of sweet potato fructose syrup preparation method according to claim 3, which is characterized in that the high-purity refines sweet potato
In glucose syrup, final concentration of 45 mg/kg of epsom salt, final concentration of 0.001 mol/L of cobalt chloride, sodium pyrosulfite are whole
Concentration is 100 ppm；The glucose isomerase additional amount is 12 mg/g substrates.
7. according to a kind of any sweet potato fructose syrup preparation method of Claims 2 or 3, which is characterized in that the syrup
Decoloration process the following steps are included:
To be cooled to decolouring liquid glucose 50 DEG C hereinafter, adjust pH=4.0~4.5, be added 303 sugared Powdered Activated Carbons, 50 DEG C ±
2 DEG C of 30 min of intermittent stirring carry out adsorption bleaching to liquid glucose；After decoloration, it is true that diatomite is carried out in conjunction with super-cell
Empty pump filters out charcoal；
Specific process is filtered by vacuum in the diatomite are as follows: a filter paper is put in Buchner funnel, the sugar diatomite that will be modulated
Equably layer overlay, then it is poured slowly into liquid glucose, carry out suction filtration removal of impurities.
8. according to a kind of any sweet potato fructose syrup preparation method of claim 2-3, which is characterized in that the syrup is washed
De- concentration technology the following steps are included:
Ion exchange desalination bleaching: suitable resin cation and resin anion (R.A.) are selected；Liquid glucose is flowed through with the flow velocity of 1.5 L/h
Sun-male-female-anion-exchange column collects liquid glucose until detection liquid glucose conductivity is less than 20 μ S/cm；
Vacuum evaporation: by the liquid glucose after desalination of decolourizing in carrying out vacuum evaporation, being concentrated into required solid content,
And make light transmittance up to 90% or more.
9. a kind of sweet potato fructose syrup preparation method according to claim 7, which is characterized in that 303 sugar is living with powder
Property charcoal additional amount be 3wt%；The sugar diatomite modulator approach are as follows: thick type diatomite and thin type silicon is added in 1:1 in mass ratio
Diatomaceous earth adds 3wt% distilled water to stir and evenly mix.
10. a kind of sweet potato fructose syrup preparation method according to claim 8, which is characterized in that the resin cation is
732 cation exchange resins, D101 macroporous absorbent resin, three kinds of resins of DM11 macroporous absorbent resin 1:1:1 ratio in mass ratio
Mixing；The resin anion (R.A.) is 201X7 strong-basicity styrene series anion exchange resin.
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| CN110408666A (en) * | 2019-08-30 | 2019-11-05 | 安徽鸿昌糖业科技有限公司 | A kind of preparation method of fructose syrup |
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