CN109607824A - A kind of biological nitrogen fixation processing method of high ammonia nitrogen organic wastewater - Google Patents
A kind of biological nitrogen fixation processing method of high ammonia nitrogen organic wastewater Download PDFInfo
- Publication number
- CN109607824A CN109607824A CN201811600132.2A CN201811600132A CN109607824A CN 109607824 A CN109607824 A CN 109607824A CN 201811600132 A CN201811600132 A CN 201811600132A CN 109607824 A CN109607824 A CN 109607824A
- Authority
- CN
- China
- Prior art keywords
- ammonia nitrogen
- high ammonia
- organic wastewater
- mycoprotein
- processing method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
Abstract
The invention discloses a kind of biological nitrogen fixation processing methods of high ammonia nitrogen organic wastewater, belong to field of environment engineering technology.The present invention includes microorganism plus rich, tame step, biological nitrogen fixation-mycoprotein synthesis step, mycoprotein separating step and filtrate biochemical treatment step, ammonia nitrogen (free ammonia and ammonium ion) in waste water is used to synthesize the protein of own growth needs using acidophilia reducing bacteria and filamentous fungi, conversion metabolism generates mycoprotein under enzyme effect in vivo, and Klebsiella can be realized high-performance bio flocculation, to achieve the purpose that remove ammonia nitrogen in waste water, and mycoprotein is recycled using solid-liquid separation method, for high quality organic fertilizer, the recyclings purposes such as feedstuff, ammonia nitrogen removal frank is 90% or more in waste water.
Description
Technical field
The invention belongs to field of environment engineering technology, solid more specifically to a kind of biology of high ammonia nitrogen organic wastewater
Nitrogen processing method.
Background technique
Ammonia nitrogen is one of the important indicator of water environment pollution object control, and the pollution generated to water body is mainly shown as water body
Eutrophication, not only reduces that water body is ornamental and use value, while being oxidized to NO3-N and NO2-N and seriously affecting water
Life is biological and endangers human health, therefore country is strict with every profession and trade ammonia nitrogen in waste water discharge standard, and such as " sewage is comprehensive
Closing discharge standard " (GB8978-1996) firsts and seconds standard ammonia nitrogen emission limit is respectively 15mg/L and 25mg/L, " poultry
Poultry breeding industry pollutant emission standard " (GB18596-2001) ammonia nitrogen emission limit be 80mg/L.
High ammonia-nitrogen wastewater refers to that ammonia-nitrogen content is higher than the waste water of 1000mg/L, while high-content is also presented (greatly in COD in waste water
In 20000mg/L), such as livestock and poultry cultivation waste waste water, kitchen garbage three phase separation waste water, landfill leachate, have processing difficult
Degree is big, the high feature of processing cost.For handling kitchen garbage three phase separation waste water, ammonia nitrogen in sewage content is 3000mg/
L, COD content are 20000mg/L, and ton water makes an investment in 5~100,000 yuan, and processing cost is at 40~50 yuan, and ammonia nitrogen index is difficult to do
To comprehensive discharge standard second level emission request.
Traditional high ammonia nitrogen processing method has ammonia aeration, nitrification -- denitrification biological denitrogenation method, break point chlorination, ion exchange
Method etc..Ammonia aeration technique efficiencies of nitrogen removal is influenced by pH, temperature, can only be as the pre-treatment of high-concentration ammonia nitrogenous wastewater, and stripping mistake
Since gas-liquid is bigger in journey, the ammonia emitted absorbs difficulty, is emitted into atmosphere and will cause two since concentration is lower
Secondary pollution.Chinese Patent Application No. 201210051165.2, data of publication of application are to disclose a kind of high ammonia on July 18th, 2012
The method of nitrogen wastewater treatment, this method are anti-by mechanical grille slot, oil separator, conditioning tank, carbon source tank, efficient combination air bearing, CASS
The structures such as device, intermediate pool, MBR contact-oxidation pool, secondary settling tank, alkali tank are answered to constitute, process route is complicated, and Institute of Micro-biology
It is lower to bear ammonia nitrogen concentration, influent ammonium concentration is lower than 500mg/L, and requirement of the nitrification and denitrification to dissolved oxygen is high, instead
Nitrifying process needs to add carbon source, and lye need to be replenished in time in nitration reaction consumption basicity, has processing cost height, technique requirement
The deficiencies of stringent.Ion-exchange has no corresponding report in high ammonia-nitrogen wastewater processing because of its expensive processing and regeneration cost
Road.
Chinese patent disclose (bulletin) number CN101643271A prior art discloses a kind of efficient wastewater biological denitrificaions
Method, the processing suitable for nitrogenous industrial wastewater, nitrogenous sanitary sewage.This method is by bacillus with percent by volume
5%~10% is seeded to seed culture medium, is 30~35 DEG C in temperature, shaking speed is cultivated under the conditions of being 180~200r/min
1~1.5d is activated.Seed liquor is taken again, is seeded to fermentation medium by the 5%~10% of percent by volume and is amplified
1~2d of fermented and cultured, until the number of bacterium is more than 10 in culture solution8Required probiotics are made in a/L.This is micro-
Ecological agent is added by the 5%~10% of percent by volume into nitrogenous effluent.This method is not necessarily to carry out the domestication of nitrobacteria
With separate, time and cost is greatly saved, while reducing workload；Still there is pole under conditions of high ammonia nitrogen initial concentration
High ammonia nitrogen removal frank can in a short time remove the nitrogen in waste water.But from the viewpoint of the embodiment, when ammonia nitrogen concentration is
When 200mg/L, removal rate 85%, and being incremented by with ammonia nitrogen concentration, removal rate is on a declining curve, and practical high ammonia nitrogen is useless
Water ammonia-nitrogen content is more than 500mg/L, and such as feces of farm waste water, ammonia-nitrogen content is greater than 1000mg/L, therefore this method is used for
It how unknown handles actual waste water effect, and from the viewpoint of the embodiment, is all made of the simulated wastewater of laboratory configuration, pollution
Ingredient is single, and microorganism growing environment is good, but actual waste water complicated component, often suspended matter (SS), COD, and there are also other dirts
Object is contaminated, as there is a certain amount of antibiotic in animal farm wastewater, if can have an impact to microbial growth, denitrification effect nothing
From textual criticism.Meanwhile in such a way that microorganism individually adds, the complexity of operating cost and operation is on the one hand increased, it is another
Aspect microorganism and high ammonia-nitrogen wastewater do not formed it is good system coexists, it is poor to the water quality resiliency that changes, influence denitrogenation
Efficiency.
Prior art CN105417778A discloses a kind of method of quick treatment scale cattle farm waste waste water, utilizes
Specific microorganism (citric acid heterotroph F1 saves number: CGMCCNo.3032)) biological flocculant is generated, by adsorbing bridge
Connection effect, charge neutralization and volume sweep effect polymerize with SS, COD, ammonia nitrogen, the total phosphorus in the waste waste water of cattle farm and
Precipitating, is further separated using solid-liquid separating equipment, and for the waste sediment isolated for producing organic fertilizer, that isolates is useless
Water realizes qualified discharge after the processing of further biological oxidation.It is anti-in biological coacervation pond and biological coagulation in the prior art
Ying Chizhong is required to that citric acid heterotroph F1 is added, and can realize and preferably remove COD, ammonia nitrogen and SS effect, through life
After the reaction of object coagulation and slagging-off, SS, COD, the total phosphorus overwhelming majority are removed in waste waste water, and removal rate is 90% or more, ammonia nitrogen
Removal rate is 70% or more.How to simplify processing step, reduces the equipment cost in production, while improving ammonia nitrogen removal
Effect is the technical problem urgently to be resolved using such method processing ammonia nitrogen waste water.
Summary of the invention
1, it to solve the problems, such as
Existing ammonia nitrogen removal is ineffective when handling high concentrated organic wastewater for the prior art causes at subsequent bio
Reason complex process and water outlet are difficult to the technical bottleneck of qualified discharge, and the biology that the present invention provides a kind of high ammonia nitrogen organic wastewater is solid
Nitrogen processing method, solve microbial acclimation during the time it is long, it is complicated for operation, be difficult to handle high ammonia-nitrogen wastewater (ammonia nitrogen >
Harmful substance acts on Antimicrobial in deficiency and actual waste water 1000mg/L), and in synchronous high-efficiency removal waste water
Other pollutants, the ammonia nitrogen in waste water is turned waste into wealth and is converted into mycoprotein, is recycled and is realized using solid-liquid separation method
Resource utilization.
2, technical solution
To solve the above problems, the present invention adopts the following technical scheme that.
A kind of biological nitrogen fixation processing method of high ammonia nitrogen organic wastewater, comprising the following steps:
(1) microorganism plus rich, domestication: specific microorganism acidophilia reducing bacteria, the Cray primary that will be isolated from sludge
Salmonella and filamentous fungi are seeded in culture medium respectively, tame after expanding numerous culture, then in high ammonia nitrogen organic wastewater, and repeatedly 3
Secondary, gained microorganism is microorganism after domestication；
(2) biological nitrogen fixation-mycoprotein synthesis: by microbial inoculum after domestication described in step (1) and high ammonia to be processed
Nitrogen waste water is inoculated with, and cultivation and fermentation obtains the muddy water containing mycoprotein；
(3) mycoprotein separates: using solid-liquid separation method by solid-liquid in the muddy water containing mycoprotein described in step (2)
Separation obtains mycoprotein mud cake and underload pollution water outlet.
Preferably, it is tamed described in step (1) are as follows: acidophilia reducing bacteria, filamentous fungi and Cray after numerous culture will be expanded
Primary Salmonella with volume ratio be (3~6): 1:(2~3) mixing, be added into high ammonia nitrogen organic wastewater to be processed, in 25~28 DEG C,
The numerous culture of oscillation expansion obtains cell density greater than 10 in the reciprocal shaker of 180~200 rpm8The inoculum of a/mL.
Preferably, the high ammonia-nitrogen wastewater COD concentration is≤20000mg/L, and ammonia nitrogen concentration is≤2000mg/L, SS content
For≤30000mg/L.
Preferably, the acidophilia reducing bacteria is Acidiphilium sp.JZ-6 (CGMCC No.11036), is belonged to thermophilic
Acid Gram-negative amphimicrobian type bacterium.
Preferably, the filamentous fungi is Mucor sp.ZG-3 (gene login sequence number is KM668056.1), belongs to hair
Mould category.
Preferably, the Klebsiella is Klebsiella sp.F1 (CGMCC NO.3032).
Preferably, step (1) acidophilia restores bacterium culture medium content are as follows: FeSO4·7H2O 1g/L, (NH4)2SO4
3.5 g/L, KCl 0.119g/L, sulphur powder 0.2g/L, K2HPO40.58g/L, Ca (NO3)2·4H2O 0.168g/L, MgSO4·
7H2O 4.42g/L。
Preferably, when the also pollutants such as SS, COD in the high ammonia-nitrogen wastewater of actual treatment, step (3) is discharged and are entered
Simple biochemical processing process, the biochemical processing process are one or more of techniques such as A/O or SBR or artificial swamp,
Being discharged each contamination index (SS, COD, ammonia nitrogen, total nitrogen, total phosphorus) can reach emission request.
Preferably, the filamentous fungi culture medium is potato dextrose broth: taking peeled potatoes 200g, is cut into small
Block adds distilled water 1000mL to boil 30min, with 8 layers of filtered through gauze murphy juice, filters off potato block, distilled water is added to supplement filtrate body
Product adds glucose 20g, uniformly dispenses 115 DEG C of high pressure steam sterilization 30min into triangular flask again after to be dissolved to 1000mL.
Preferably, the citric acid bacterium culture medium content are as follows: glucose 10g/L, calcium lactate 5g/L.
Preferably, step (1) the acidophilia reducing bacteria and Klebsiella expand numerous culture be respectively placed in 25~28 DEG C,
Numerous culture is expanded in oscillation in the reciprocal shaker of 180~200 rpm, until somatic cells quantity reaches 108A/mL.
Preferably, filamentous fungi described in step (1) expands the extraction and fungal inocula that numerous culture includes fungal spore
Preparation；The extraction of fungal spore includes: in an aseptic environment, to add sterile water to the plate with fresh filamentous fungi, use glass
Glass, which applies stick agitation, rinses fungal spore, and filtering is placed in centrifuge tube, saves at 4 DEG C；The preparation packet of fungal inocula
It includes: aseptically, spore suspension being diluted to 10 with sterile water7A/mL, spore suspension is inoculated with after taking 1mL to dilute
To equipped in the sterilized PDB culture medium of 99mL, be placed in 25~28 DEG C, numerous training is expanded in oscillation in 180~200rpm reciprocal shaker
It supports, until somatic cells quantity reaches 108A/mL.
Preferably, the volume ratio of mixed bacteria liquid and high ammonia nitrogen organic wastewater is 1:3 during step (1) described domestication.
Preferably, the inoculation volume ratio of microbial inoculum and high ammonia-nitrogen wastewater to be processed is (3 after step (2) described domestication
~5): 20.
Preferably, using the thallus after domestication described in centrifugal process separating step (1).
Preferably, step (2) the cultivation and fermentation condition is 28~30 DEG C, under aerobic condition, and aeration quantity is 3~8m3/
(h·m2)。
Preferably, a length of 12~36h, system pH reach 5.5 or less when step (2) described cultivation and fermentation.
Preferably, step (3) the isolated mycoprotein mud cake dry at low temperature and be broken into 0.1~
0.5cm particle.
Preferably, step (3) mycoprotein mud cake low temperature drying temperature is 40~45 DEG C.
Preferably, step (3) solid-liquid separation method is gravity concentration or mechanical dehydration method.
Preferably, step (3) solid-liquid separation method is plate compression dehydration.
Preferably, it is low to refer to that ammonia nitrogen concentration is distinguished lower than 100mg/L, COD and SS for step (3) the low pollution load water outlet
In the water quality of 300mg/L and 50mg/L.
3, beneficial effect
Compared with the prior art, the invention has the benefit that
(1) present invention by nitrogen-fixing microorganism flora by the mineralized nitrogen in high ammonia nitrogen organic wastewater at mycoprotein reality
The purpose now removed handles high ammonia nitrogen organic wastewater using composite flora, overcomes use using the synergistic effect of composite flora
Single microorganism buffering bad adaptability causes the organic wastewater higher for ammonia nitrogen concentration such as kitchen garbage, waste-water NH_3-N treating to imitate
The technology limitation that fruit is poor, COD, SS removal rate are low, can rapidly and efficiently cooperate with processing ammonia nitrogen, COD and SS pollutant, and a step makes height
Concentration organic wastewater is reduced to low pollution load sewage, substantially reduces the technology difficulty of subsequent biological treatment to guarantee that water outlet reaches
Mark rate.
(2) present invention is true by specific microorganism acidophilia reducing bacteria, Klebsiella and the filiform isolated from sludge
Bacterium is seeded in culture medium, tames after expanding numerous culture, then in high ammonia nitrogen organic wastewater, also using the acidophilia after domestication
Opportunistic pathogen, filamentous fungi and Klebsiella composite bacteria liquid are carbon source using the inorganic substances in waste water, the ammonia nitrogen (trip in waste water
From ammonia and ammonium ion) enter in microorganism growth process internal and is reacted under glutamine synthelase effect with glutamic acid
Glutamine is generated, transaminase-catalyzed glutamine occurs transamination and then synthesizes other amino acid, and a variety of amino acid are made
Precursor substance for protein synthesis catalyzes and synthesizes microbial bacteria body protein by protein synzyme；Wherein, gram of use
The primary Salmonella F1 of thunder can generate high-performance bio flocculant, lead to adsorption bridging effect capture waste water in the form of inertia existing for
COD, ammonia nitrogen, SS substance, the mycelia of filamentous fungi can adsorb dissolubility and non-solubility substance in high ammonia-nitrogen wastewater, and lead to
The biomass for being metabolized these substances to increase itself is crossed, for synthesizing the protein of own growth needs, in vivo under enzyme effect
Conversion metabolism generates mycoprotein, thus achieve the purpose that cooperate with ammonia nitrogen in removal waste water with Klebsiella, meanwhile, it is Filamentous
The mycelia of fungi by absorption, absorb and squeeze in waste water with the poroid of pollutant existing for inertia form (COD, SS etc.)
Architectural characteristic plays the role of flocculating and settling；System acidity is effectively reduced during converting ammonia nitrogen in acidophilia reducing bacteria,
To avoid metabolic influence of the harmful substances such as antibiotic on filamentous fungi conversion ammonia nitrogen in waste water, further ensure
Klebsiella；Under acidophilia reducing bacteria, Klebsiella and the collective effect of filamentous fungi, thallus egg is generated with ammonia nitrogen
It is white, mycoprotein is recycled using solid-liquid separation method, the high-quality for being conducive to mycetome albumen mud cake after mud-water separation is organic
Fertilizer, feedstuff resource utilization.
(3) used in technical solution of the present invention acidophilia reducing bacteria JZ-6, filamentous fungi ZG-3 and Klebsiella F1 with
Volume ratio is (3~6): 1:(2~3), and obtain COD, ammonia nitrogen, SS substance removal rate in this proportional region and have larger mention
It is high；In embodiment 1, acidophilia reducing bacteria JZ-6, filamentous fungi ZG-3 and Klebsiella F1 are 3:1:2, COD with volume ratio
Removal rate reaches 97.2%, and ammonia nitrogen removal frank 96.4%, SS removal rate is 98.9%, and comparative example A is under equal conditions,
Use acidophilia reducing bacteria JZ-6, filamentous fungi ZG-3 and Klebsiella F1 with volume ratio be 1:1:1 when, ammonia nitrogen removal
It is 87%, SS removal rate is 89% that rate, which is 87.5%, COD removal rate,；In comparative example B, using acidophilia reducing bacteria JZ-6, silk
When shape fungi ZG-3 and Klebsiella F1 with volume ratio is 6:1:1, ammonia nitrogen removal frank 83%, COD removal rate is
80%, SS removal rate are 85%；In comparative example C, using acidophilia reducing bacteria JZ-6, filamentous fungi ZG-3 and Klebsiella
F1 with volume ratio be 1:1:3 when, ammonia nitrogen removal frank 81%, COD removal rate is that 86%, SS removal rate is 88%；It can push away
Know, it is compound micro- when the volume ratio of acidophilia reducing bacteria JZ-6 and Klebsiella F1 and filamentous fungi ZG-3 reach special ratios
Biology can reach optimal ammonia nitrogen removal effect, realize that ammonia nitrogen removal frank is 90% or more in waste water, compared to single Cray primary
The microbiological treatment system of Salmonella F1 is greatly improved.
(4) microbial bacteria body protein of the present invention shifts during the fermentation is attached to suspended particulate substance surface, citric acid
The biological flocculant of bacterium secretion has good flocculation coagulation performance, further realizes mud-water separation by solid-liquid separation method,
0.1~0.5cm particle is dried at low temperature and be broken into mycetome albumen mud cake, utilizes convenient for following resourceization.
(5) ammonia nitrogen removal frank is 90% or more after biological nitrogen fixation is handled for the high ammonia nitrogen organic wastewater of the present invention, while through solid
Liquid separation process ss suspended solid removal rate is in 95% or more, COD removal rate 80% or more；It is big for ammonia nitrogen concentration in water inlet
In 2000mg/L or more, filtrate after separation of solid and liquid is discharged into Easy manual's wetland variation, water outlet can qualified discharge.
Detailed description of the invention
Fig. 1 is Examples 1 to 3 technical solution process flow chart.
Fig. 2 is 4 technical solution process flow chart of embodiment.
Fig. 3 is the technical solution process flow chart of embodiment 5 and 6.
Specific embodiment
The present invention is further described below combined with specific embodiments below.
Embodiment 1
According to 1 process of attached drawing to initial concentration are as follows: ammonia nitrogen: 500mg/L, COD:18900mg/L, SS:24800mg/L's
Ammonia nitrogen waste water is handled.
(1) microorganism plus rich, domestication: acidophilia reducing bacteria JZ-6, the Klebsiella F1 that will be isolated from sludge
It is seeded in culture medium with filamentous fungi ZG-3, is subsequently placed in 28 DEG C, numerous culture is expanded in oscillation in the reciprocal shaker of 180rpm,
Until somatic cells quantity reaches 108A/mL；
Acidophilia restores bacterium culture medium content g/L:FeSO4·7H2O 1g/L, (NH4)2SO43.5g/L, KCl 0.119g/
L, sulphur powder 0.2g/L, K2HPO40.58g/L, Ca (NO3)2·4H2O 0.168g/L, MgSO4·7H2O 4.42g/L。
Filamentous fungi ZG-3 seeded process are as follows: (1) extraction of fungal spore: in an aseptic environment, addend milliliter sterile water
On to the plate with fresh filamentous fungi, applying stick agitation with glass rinses fungal spore, and filtering is placed in centrifuge tube
In, it is saved at 4 DEG C；(2) preparation of fungal inocula: aseptically, spore suspension is diluted to sterile water
107A/mL, spore suspension is seeded to equipped in the sterilized PDB culture medium of 99mL after taking 1mL to dilute.
Klebsiella F1 culture medium content are as follows: glucose 10g/L, calcium lactate 5g/L.
Acidophilia reducing bacteria JZ6, filamentous fungi ZG-3 and Klebsiella F1 are mixed by 3:1:2, draw Mixed Microbes
For liquid into waste water to be processed, the volume ratio of mixed bacteria liquid and high ammonia nitrogen organic wastewater is 1:3, in 28 DEG C, 180rpm it is reciprocating
The numerous culture of oscillation expansion obtains cell density greater than 10 in shaking table8A/mL, using centrifugal process separating thallus, gained thallus is again in height
It is tamed in ammonia nitrogen organic wastewater, 3 times repeatedly, gained microorganism is microorganism after domestication.
(2) biological nitrogen fixation-mycoprotein synthesis: by microbial inoculum after domestication and waste water to be processed by volume 15% into
Row inoculation is placed in 28 DEG C, (aeration quantity 3m under aerobic condition3/(h·m2)) carry out cultivation and fermentation, the reaction system pH after 12h
Reach 5.4, fermentation process terminates.
(3) mycoprotein separates: fermentation liquid being realized mud-water separation by solid-liquid separating machine, selected mesh size is 100
Mesh.The mycetome albumen mud cake obtained after separation of solid and liquid dries 2h under low temperature baking oven, and temperature control is at 45 DEG C, moisture content of the cake
Lower than 60%.Mud cake dries and is broken into particle of the partial size between 0.1~0.5cm with crusher, can be used as high-quality
Composting material.Clear filtrate is obtained after separation of solid and liquid.The ammonia-nitrogen content in filtrate is 18mg/L after measured, and removal rate is
96.4%；COD removal rate is that 97.2%, SS removal rate is 98.9%.
Comparative example A
It is essentially identical with technical solution in embodiment 1 in this example, identical waste water is handled, difference place is: acidophilia is also
Opportunistic pathogen JZ-6, filamentous fungi ZG-3 and Klebsiella F1 are 1:1:1 with volume ratio (total volume is still in the same manner as in Example 1).
Filtrate after measured, ammonia-nitrogen content 62.5mg/L, removal rate 87.5%；COD removal rate is 87%, SS
Removal rate is 89%.
Comparative example B
It is essentially identical with technical solution in embodiment 1 in this example, identical waste water is handled, difference place is: acidophilia is also
Opportunistic pathogen JZ-6, filamentous fungi ZG-3 and Klebsiella F1 are 6:1:1 with volume ratio (total volume is still in the same manner as in Example 1).
Filtrate after measured, ammonia-nitrogen content 85mg/L, removal rate 83%；COD removal rate is that 80%, SS is gone
Except rate is 85%.
Comparative example C
It is essentially identical with technical solution in embodiment 1 in this example, identical waste water is handled, difference place is: acidophilia is also
Opportunistic pathogen JZ-6, filamentous fungi ZG-3 and Klebsiella F1 are 1:1:3 with volume ratio (total volume is still in the same manner as in Example 1).
Filtrate after measured, ammonia-nitrogen content 95mg/L, removal rate 81%；COD removal rate is that 86%, SS is gone
Except rate is 88%.
Embodiment 2
According to 1 process of attached drawing to initial concentration are as follows: ammonia nitrogen: 1000mg/L, COD:21700mg/L, SS:28900mg/L's
Ammonia nitrogen waste water is handled.
(1) microorganism plus rich, domestication: with embodiment 1, difference is step: acidophilia reducing bacteria JZ6, filamentous fungi
The volume ratio of ZG-3 and Klebsiella F1 are 5:1:2.5.
(2) biological nitrogen fixation-mycoprotein synthesis: by microbial inoculum after domestication and waste water to be processed by volume 20% into
Row inoculation is placed in 28 DEG C, (aeration quantity 6m under aerobic condition3/(h·m2)) carry out cultivation and fermentation, the reaction system pH after for 24 hours
Reach 5.2, fermentation process terminates.
(3) mycoprotein separates: fermentation liquid being realized mud-water separation by solid-liquid separating machine, selected mesh size is 100
Mesh.The mycetome albumen mud cake obtained after separation of solid and liquid dries 2h under low temperature baking oven, and temperature control is at 45 DEG C, moisture content of the cake
Lower than 60%.Mud cake dries and is broken into particle of the partial size between 0.1~0.5cm with crusher.It is obtained after separation of solid and liquid
Obtain clear filtrate.The ammonia-nitrogen content in filtrate is 55mg/L, removal rate 94.5% after measured；COD removal rate is 93%, SS
Removal rate is 98%.
Embodiment 3
According to 1 process of attached drawing to initial concentration are as follows: ammonia nitrogen: 2000mg/L, COD:25700mg/L, SS:29800mg/L's
Ammonia nitrogen waste water is handled.
(1) microorganism plus rich, domestication: with embodiment 1, difference is operating procedure: acidophilia reducing bacteria JZ6, filiform
The volume ratio of fungi ZG-3 and Klebsiella F1 are 6:1:3.
(2) biological nitrogen fixation-mycoprotein synthesis: by microbial inoculum after domestication and waste water to be processed by volume 25% into
Row inoculation is placed in 28 DEG C, (aeration quantity 8m under aerobic condition3/(h·m2)) cultivation and fermentation is carried out, reaction system pH reaches after 36h
To 5.4, fermentation process terminates.
(3) mycoprotein separates: fermentation liquid being realized mud-water separation by solid-liquid separating machine, selected mesh size is 100
Mesh.The mycetome albumen mud cake obtained after separation of solid and liquid dries 2h under low temperature baking oven, and temperature control is at 45 DEG C, moisture content of the cake
Lower than 60%.Mud cake dries and is broken into particle of the partial size between 0.1~0.5cm with crusher.It is obtained after separation of solid and liquid
Obtain clear filtrate.The ammonia-nitrogen content in filtrate is 125mg/L, removal rate 93.8% after measured；COD removal rate is 90%, SS
Removal rate is 96%.
Embodiment 4
The present embodiment is nitrification liquid processing after certain kitchen garbage treatment plant anaerobic nitrification.
Mentioned technique according to the present invention, is handled digestive juice after factory's anaerobic digestion according to 2 process of attached drawing, should
Digestive juice basic physical and chemical is as follows:
(1) microorganism plus rich, domestication: with embodiment 1, difference is operating procedure: acidophilia reducing bacteria JZ6, filiform
The volume ratio of fungi ZG-3 and Klebsiella F1 are 4:1:2.
(2) biological nitrogen fixation-mycoprotein synthesis: by digestive juice after microbial inoculum after domestication and anaerobic digestion of kitchen wastes
It 25% is inoculated with by volume, is placed in 28 DEG C, (aeration quantity 8m under aerobic condition3/(h·m2)) cultivation and fermentation is carried out, through 36h
Reaction system pH reaches 5.3 afterwards, and fermentation process terminates.
(3) mycoprotein separates: fermentation liquid being realized mud-water separation by solid-liquid separating machine, selected mesh size is 100
Mesh.The mycetome albumen mud cake obtained after separation of solid and liquid dries 2h under low temperature baking oven, and temperature control is at 45 DEG C, moisture content of the cake
Lower than 60%.Mud cake dries and is broken into particle of the partial size between 0.1~0.5cm with crusher.It is obtained after separation of solid and liquid
Clear filtrate is obtained, each pollutant index is as follows after measured:
(4) filtrate biochemical treatment: carrying out biochemical treatment using A/O technique to filtrate after separation of solid and liquid, handle time 2d, right
Discharge outlet water outlet is measured, and each pollution determination value is as follows:
Certain kitchen garbage, waste-water of table 1 compares before and after the processing
The secondary standard in " integrated wastewater discharge standard " (GB8978-1996) is compareed, final outflow water meets discharge and wants
It asks.
Embodiment 5
The present embodiment is Nanjing cattle farm waste wastewater treatment.
Cattle farm waste waste water is handled according to 3 process of attached drawing, waste to be processed is cattle farm by being separated by solid-liquid separation
The underflow water generated afterwards, basic physics and chemistry matter: pH7.65, SS, COD, NH3The content of-N and total phosphorus (TP) is respectively 69500,
91470,957 and 129mg/L.
(1) microorganism plus rich, domestication: with embodiment 1.
(2) biological nitrogen fixation-mycoprotein synthesis: by microbial inoculum after domestication and cattle farm underflow water by volume 15%
It is inoculated with, is placed in 28 DEG C, (aeration quantity 3m under aerobic condition3/(h·m2)) carry out cultivation and fermentation, the reaction system after 12h
PH reaches 5.2, and fermentation process terminates.
(3) mycoprotein separates: fermentation liquid is realized mud-water separation, feed time 0.5h, high pressure by plate and frame filter press
Hydraulic pressure squeezes pressure maintaining 10min, feed pressure 0.4MPa, diaphragm pressing pressure 0.6MPa.The mycetome albumen obtained after separation of solid and liquid
Mud cake dries 4h under low temperature baking oven, and at 40 DEG C, moisture content of the cake is lower than 60% for temperature control.Mud cake is dried and is used broken
Particle of the crusher machine at partial size between 0.1~0.5cm.Clear filtrate is obtained after separation of solid and liquid, after measured each pollutant index
It is as follows: SS, COD, NH3The content of-N and total phosphorus (TP) is respectively 200,4500,68 and 18mg/L.
(4) filtrate biochemical treatment: carrying out biochemical treatment using SBR technique to filtrate after separation of solid and liquid, handle time 3d,
Discharge outlet water outlet is measured, each pollution determination value is as follows: SS, COD, NH3The content of-N and total phosphorus (TP) is respectively
30,102,25 and 5mg/L.It compares " livestock and poultry breeding industry pollutant emission standard " (GB18596-2001), final outflow water meets row
Requirement is put, can be used for cattle farm itself cowshed rinse water and greening water etc..
Comparison before and after certain the cattle farm waste wastewater treatment of table 2
Embodiment 6
The present embodiment is Hunan piggery waste wastewater treatment.
Piggery waste waste water is handled according to 3 process of attached drawing, the substantially physical and chemical matter of waste waste water to be processed is as follows: pH=
7.52, SS, COD, NH3The content of-N and total phosphorus (TP) is respectively 4900,11290,1044 and 148mg/L.
(1) microorganism plus rich, domestication: with embodiment 1.
(2) biological nitrogen fixation-mycoprotein synthesis: by microbial inoculum after domestication and piggery waste waste water by volume 40%
It is inoculated with, is placed in 28 DEG C, (aeration quantity 5m under aerobic condition3/(h·m2)) carry out cultivation and fermentation, the reaction system after for 24 hours
PH reaches 5.2, and fermentation process terminates.
(3) mycoprotein separates: fermentation liquid is realized mud-water separation, feed time 0.5h, high pressure by plate and frame filter press
Hydraulic pressure squeezes pressure maintaining 10min, feed pressure 0.4MPa, diaphragm pressing pressure 0.6MPa.The mycetome albumen obtained after separation of solid and liquid
Mud cake dries 4h under low temperature baking oven, and at 40 DEG C, moisture content of the cake is lower than 60% for temperature control.Mud cake is dried and is used broken
Particle of the crusher machine at partial size between 0.1~0.5cm.Clear filtrate is obtained after separation of solid and liquid, after measured each pollutant index
It is as follows: SS, COD, NH3The content of-N and total phosphorus (TP) is respectively 100,1500,58 and 8mg/L.
(4) filtrate biochemical treatment: carrying out biochemical treatment using SBR technique to filtrate after separation of solid and liquid, handle time 2d,
Discharge outlet water outlet is measured, each pollution determination value is as follows: SS, COD, NH3The content of-N and total phosphorus (TP) is respectively
50,125,30 and 3mg/L.It compares " livestock and poultry breeding industry pollutant emission standard " (GB18596-2001), final outflow water meets row
Requirement is put, can be used for cattle farm itself cowshed rinse water and greening water etc..
Comparison before and after certain the piggery waste wastewater treatment of table 3
By above-mentioned data it is found that joint acidophilia reducing bacteria JZ6, filamentous fungi ZG3 and Klebsiella F1 handle high ammonia
Nitrogen waste water, the ammonia nitrogen that can be absorbed and utilized in waste water by microorganism is used to synthesize itself mycoprotein as energy substance, both real
Show the purpose of efficient degradation ammonia nitrogen, and be converted into the mycoprotein more preferably recycled, realizes waste resource recovery.
Claims (10)
1. a kind of biological nitrogen fixation processing method of high ammonia nitrogen organic wastewater, which comprises the following steps:
(1) microorganism plus rich, domestication: specific microorganism acidophilia reducing bacteria, the Klebsiella that will be isolated from sludge
It is seeded in culture medium, is tamed after expanding numerous culture, then in high ammonia nitrogen organic wastewater, 3 times repeatedly, institute respectively with filamentous fungi
Obtaining microorganism is microorganism after domestication；
(2) biological nitrogen fixation-mycoprotein synthesis: microbial inoculum after domestication described in step (1) and high ammonia nitrogen to be processed is useless
Water is inoculated with, and cultivation and fermentation obtains the muddy water containing mycoprotein；
(3) mycoprotein separates: being divided solid-liquid in the muddy water containing mycoprotein described in step (2) using solid-liquid separation method
From, obtain mycoprotein mud cake and underload pollution water outlet.
2. the biological nitrogen fixation processing method of high ammonia nitrogen organic wastewater according to claim 1, it is characterised in that: step (1)
Described in tame are as follows: acidophilia reducing bacteria, filamentous fungi and Klebsiella after numerous culture will be expanded with volume ratio as (3~6):
1:(2~3) mixing, it is added into high ammonia nitrogen organic wastewater to be processed, in 25~28 DEG C, the reciprocal shaker of 180~200rpm
Middle oscillation expands numerous culture and obtains cell density greater than 108The inoculum of a/mL.
3. the biological nitrogen fixation processing method of high ammonia nitrogen organic wastewater according to claim 1, it is characterised in that: when practical place
There are also when the pollutants such as SS, COD in the high ammonia-nitrogen wastewater of reason, step (3) are discharged and enter simple biochemical processing process, it is described
Biochemical processing process is one or more of techniques such as A/O or SBR or artificial swamp, and being discharged each contamination index can reach discharge
It is required that.
4. the biological nitrogen fixation processing method of high ammonia nitrogen organic wastewater according to claim 2, it is characterised in that: the filiform
Fungi ZG-3 culture medium is potato dextrose broth: taking peeled potatoes 200g, is cut into small pieces, distilled water 1000mL is added to boil
30min is boiled, with 8 layers of filtered through gauze murphy juice, filters off potato block, adds distilled water supplement filtrate volume to 1000mL, adds glucose
20g uniformly dispenses 115 DEG C of high pressure steam sterilization 30min into triangular flask after to be dissolved again.
5. the biological nitrogen fixation processing method of high ammonia nitrogen organic wastewater according to claim 2, it is characterised in that: step (1)
Described in filamentous fungi expand it is numerous culture include fungal spore extraction and fungal inocula preparation；The extraction packet of fungal spore
It includes: in an aseptic environment, adding sterile water to the plate with fresh filamentous fungi, applying stick agitation with glass rushes fungal spore
It washes, filtering is placed in centrifuge tube, is saved at 4 DEG C；The preparation of fungal inocula includes: that aseptically, use is sterile
Spore suspension is diluted to 10 by water7A/mL, spore suspension is seeded to equipped with the sterilized PDB training of 99mL after taking 1mL to dilute
It supports in base, is placed in 25~28 DEG C, numerous culture is expanded in oscillation in 180~200rpm reciprocal shaker, until somatic cells quantity reaches
108A/mL.
6. the biological nitrogen fixation processing method of high ammonia nitrogen organic wastewater according to claim 2, it is characterised in that: step (1)
The volume ratio of mixed bacteria liquid and high ammonia nitrogen organic wastewater is 1:3 during the domestication.
7. the biological nitrogen fixation processing method of high ammonia nitrogen organic wastewater according to claim 6, it is characterised in that: step (2)
The inoculation volume ratio of microbial inoculum and high ammonia-nitrogen wastewater to be processed is (3~5) after the domestication: 20.
8. the biological nitrogen fixation processing method of high ammonia nitrogen organic wastewater according to claim 6, it is characterised in that: step (2)
The cultivation and fermentation condition is 28~30 DEG C, under aerobic condition, and aeration quantity is 3~8m3/(h·m2)。
9. the biological nitrogen fixation processing method of high ammonia nitrogen organic wastewater according to claim 6, it is characterised in that: step (2)
A length of 12~36h when the cultivation and fermentation, system pH reach 5.5 or less.
10. the biological nitrogen fixation processing method of high ammonia nitrogen organic wastewater described according to claim 1~any one of 9, special
Sign is: step (3) the isolated mycoprotein mud cake dries at low temperature and is broken into 0.1~0.5cm particle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811600132.2A CN109607824A (en) | 2018-12-26 | 2018-12-26 | A kind of biological nitrogen fixation processing method of high ammonia nitrogen organic wastewater |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811600132.2A CN109607824A (en) | 2018-12-26 | 2018-12-26 | A kind of biological nitrogen fixation processing method of high ammonia nitrogen organic wastewater |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109607824A true CN109607824A (en) | 2019-04-12 |
Family
ID=66010655
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811600132.2A Pending CN109607824A (en) | 2018-12-26 | 2018-12-26 | A kind of biological nitrogen fixation processing method of high ammonia nitrogen organic wastewater |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109607824A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1464626A2 (en) * | 2003-03-28 | 2004-10-06 | Biovitis S.A. | Process for the treatment of agri-food and industrial effluents comprising one phase of biodigestion by filamentous fungi |
| US20070190632A1 (en) * | 2004-10-01 | 2007-08-16 | Cornelius Bessler | Alpha-amylase variants having an elevated solvent stability, method for the production thereof and detergents and cleansers containing these alpha-amylase variants |
| CN102476865A (en) * | 2010-11-26 | 2012-05-30 | 中国辐射防护研究院 | Wastewater denitrogenation method by use of penicillium strain |
| CN105417778A (en) * | 2015-11-26 | 2016-03-23 | 南京贝克特环保科技有限公司 | Method for quickly treating excrement wastewater in large-scale dairy farm |
| CN108128989A (en) * | 2018-02-12 | 2018-06-08 | 南京贝克特环保科技有限公司 | A kind of new bio nitrogen fixation process processing high ammonia-nitrogen wastewater device and processing method |
| CN108128996A (en) * | 2017-12-27 | 2018-06-08 | 南京贝克特环保科技有限公司 | A kind of processing complex microorganism bacterium solution of citric acid sludge and bioleaching processing method |
| CN108342339A (en) * | 2018-01-16 | 2018-07-31 | 浙江双良商达环保有限公司 | Klebsiella bacterial strain and its application of sanitary sewage containing ammonia nitrogen in river sewage and rural area |
| CN108707598A (en) * | 2018-05-29 | 2018-10-26 | 江南大学 | A method of using filamentous fungi as the intensified anti-nitrated bacterial nitrogen removal of carrier |
| CN108996832A (en) * | 2018-08-17 | 2018-12-14 | 南宁市黄陈生猪养殖场 | A kind of technique using microbial inoculum processing industrial wastewater |
-
2018
- 2018-12-26 CN CN201811600132.2A patent/CN109607824A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1464626A2 (en) * | 2003-03-28 | 2004-10-06 | Biovitis S.A. | Process for the treatment of agri-food and industrial effluents comprising one phase of biodigestion by filamentous fungi |
| US20070190632A1 (en) * | 2004-10-01 | 2007-08-16 | Cornelius Bessler | Alpha-amylase variants having an elevated solvent stability, method for the production thereof and detergents and cleansers containing these alpha-amylase variants |
| CN102476865A (en) * | 2010-11-26 | 2012-05-30 | 中国辐射防护研究院 | Wastewater denitrogenation method by use of penicillium strain |
| CN105417778A (en) * | 2015-11-26 | 2016-03-23 | 南京贝克特环保科技有限公司 | Method for quickly treating excrement wastewater in large-scale dairy farm |
| CN108128996A (en) * | 2017-12-27 | 2018-06-08 | 南京贝克特环保科技有限公司 | A kind of processing complex microorganism bacterium solution of citric acid sludge and bioleaching processing method |
| CN108342339A (en) * | 2018-01-16 | 2018-07-31 | 浙江双良商达环保有限公司 | Klebsiella bacterial strain and its application of sanitary sewage containing ammonia nitrogen in river sewage and rural area |
| CN108128989A (en) * | 2018-02-12 | 2018-06-08 | 南京贝克特环保科技有限公司 | A kind of new bio nitrogen fixation process processing high ammonia-nitrogen wastewater device and processing method |
| CN108707598A (en) * | 2018-05-29 | 2018-10-26 | 江南大学 | A method of using filamentous fungi as the intensified anti-nitrated bacterial nitrogen removal of carrier |
| CN108996832A (en) * | 2018-08-17 | 2018-12-14 | 南宁市黄陈生猪养殖场 | A kind of technique using microbial inoculum processing industrial wastewater |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108101228A (en) | A kind of wastewater treatment microbial microbial inoculum | |
| CN109912155A (en) | A kind of integrated technology and application method of livestock and poultry liquid dung harmless treatment | |
| CN104261570B (en) | A kind of livestock and poultry cultivation anaerobism purifying agent for polluted water | |
| CN113321345A (en) | Method for simultaneously recovering phosphorus in water body and removing antibiotics based on sludge-based biochar | |
| CN106967435A (en) | A kind of preparation method of multifunction soil improver | |
| CN103642729B (en) | Method for producing Bacillus subtilis for feeds by fermenting high-salt-content amino acid wastewater | |
| CN102010104A (en) | Method for treating riboflavin fermentation waste water | |
| CN107879774A (en) | A kind of preparation method of biological decomposing agent | |
| CN105130013A (en) | Method of treating piggery methane fluid by utilizing composite photosynthetic bacteria | |
| CN105198161B (en) | A kind of processing preparation suitable for high-concentration hardly-degradable waste water | |
| CN101665805A (en) | Method for producing microbial flocculant by using aquaculture wastewater | |
| LU500900B1 (en) | System and method for treating human and livestock excrement | |
| CN101701197B (en) | Novel microorganism flora mixture and mixed nutrient medium thereof | |
| CN111233196A (en) | Garbage percolation concentrated solution adsorption fermentation dehydration solidification process | |
| CN105502852B (en) | A kind of method of quick processing garbage burning factory landfill leachate | |
| CN105084557B (en) | Technology for removing industrial COD and ammonia nitrogen from monosodium glutamate wastewater | |
| CN104478184B (en) | A kind of method of municipal sludge clean utilization | |
| CN102897923A (en) | Bioleaching method for promoting deep dehydration of water-blooming cyanobacteria | |
| CN107828773A (en) | A kind of preparation method of the domestication agent of the activated sludge of brine waste processing | |
| CN105621806B (en) | A kind of biological coagulation oxidation technology of quick processing kitchen garbage, waste-water | |
| CN208684682U (en) | A kind of total system of landfill leachate tail water | |
| CN108128996B (en) | Composite microbial liquid for treating citric acid sludge and bioleaching treatment method | |
| CN110498584A (en) | A kind of municipal sludge fermentation assistant | |
| CN109607824A (en) | A kind of biological nitrogen fixation processing method of high ammonia nitrogen organic wastewater | |
| CN105754904B (en) | One plant of ocean Psychrobacter strain and its application in water body dephosphorized |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190412 |