CN102476865A - Wastewater denitrogenation method by use of penicillium strain - Google Patents
Wastewater denitrogenation method by use of penicillium strain Download PDFInfo
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- CN102476865A CN102476865A CN2010105698702A CN201010569870A CN102476865A CN 102476865 A CN102476865 A CN 102476865A CN 2010105698702 A CN2010105698702 A CN 2010105698702A CN 201010569870 A CN201010569870 A CN 201010569870A CN 102476865 A CN102476865 A CN 102476865A
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- denitrification
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Abstract
The invention relates to a wastewater biological denitrogenation technology, specifically to a wastewater denitrogenation method by the use of penicillium strain. According to the method, the organic-bearing wastewater is controlled under the technological conditions as follows: temperature is 5-40 DEG C, pH value is 5.0-9.0, and dissolved oxygen concentration is 0.5-6.5mg/L. The method comprises the following steps of: stirring the wastewater, respectively adding 0.5-300 per thousand of penicillium and progressing mixed cultivation with wastewater for 4-8 hours according to different concentration ranges and with the combination of the wastewater quantity, and accomplishing synchronization and denitrification under the effect of penicillium. By the use of penicillium to treat wastewater, the treatment efficiency is increased by more than 3 times in comparison with that by the use of other strains. The whole denitrogenation reaction process speeds up, the hydraulic retention time can be shortened, and the reactor volume also can be correspondingly reduced. The method provided by the invention has a good development and application prospect in the field of wastewater biological denitrogenation.
Description
Technical field
The present invention relates to a kind of bio-denitrifying sewage technology, be specifically related to a kind of method of utilizing the penicillium spp bacterial strain to carry out sewage water denitrification.
Background technology
Traditional theory thinks that the nitrification and denitrification effect of mikrobe is two diverse biological processes.Nitrification is generally carried out under aerobic condition, divides two stages, and accomplished by two kinds of chemoautotrophic bacterias: nitrococcus is converted into nitrite anions with ammonia, and nitrifier is oxidized to nitrate radical with nitrite anions.Denitrification is reduced to nitrogen or oxynitride with nitrate radical, nitrite anions by heterotrophic denitrifying bacteria under the condition of anaerobism or double oxygen.The respiratory chain of denitrifying bacteria can adopt oxygen under aerobic conditions be terminal electron acceptor, but only just be that terminal electron acceptor carries out anti-nitration reaction with the nitrate radical under anoxia condition.
Main two steps that adopt nitrifying process and denitrification process in traditional bio-denitrifying sewage treating processes, each step adopts different bacterial classifications, is called for short two-step approach.Along with discovering, some special bacteriums can carry out heterotrophic nitrification, and carry out aerobic denitrification simultaneously, and these bacteriums are called as the heterotrophic nitrification-aerobic denitrification bacterium.Based on this type of research, developed synchronous nitration and denitrification single stage method in one, can realize the bio-denitrifying sewage function, overcome the loaded down with trivial details and big shortcoming of energy consumption of existing operation in the traditional two-step method.The bacterial classification kind that present single stage method is adopted is more, and the present invention is primarily aimed at the treatment process that penicillium spp proposes corresponding sewage water denitrification.
Summary of the invention
The objective of the invention is to adopt penicillium spp bacterial strain to carry out the sewage water denitrification treatment process, thereby can in the biological denitrificaion of sewage is handled, realize synchronous nitration and denitrification function with heterotrophic nitrification and aerobic denitrification function.
Technical scheme of the present invention is following: a kind of method of utilizing the penicillium spp bacterial strain to carry out sewage water denitrification; To contain organic sewage is controlled under the following processing condition: temperature 5-40 ℃, pH value 5.0-9.0; Dissolved oxygen concentration 0.5-6.5mg/L stirs sewage, according to the ammonia nitrogen different concns scope in the sewage and the composite wastewater water yield; The penicillium spp and the sewage that add 0.5-300 ‰ respectively carried out mixed culture 4-8 hour, under the effect of penicillium spp, realized synchronous nitration and denitrification.
Further, the aforesaid method of utilizing the penicillium spp bacterial strain to carry out sewage water denitrification wherein, can cooperate filling carrier in the sewage, and concrete filler can adopt 3 D elastic filler+combined stuffing, is covered with contact-oxidation pool 90% and gets final product.
Further, the aforesaid method of utilizing the penicillium spp bacterial strain to carry out sewage water denitrification, wherein, through aeration system, or whisking appliance, or underwater propeller stirs sewage.
Beneficial effect of the present invention is following: the present invention disposes of sewage through utilizing penicillium spp; Nitrification and denitrification is accomplished in same reactor drum; Processing efficiency is than adopting other bacterial classification to improve more than 3 times, and whole denitrification reaction process is accelerated, and hydraulic detention time can shorten; Reactor volume also can correspondingly reduce, and has the excellent development application prospect in the wastewater biological denitrificaion field.
Embodiment
Below in conjunction with embodiment the present invention is carried out detailed description.
At temperature 5-40 ℃, pH value 5.0-9.0; Under dissolved oxygen (DO) the 0.5-6.5mg/L concentration conditions, in a pond body, add and contain organic sewage, can cooperate carriers such as filler in the sewage; Concrete filler can adopt 3 D elastic filler+combined stuffing, is covered with contact-oxidation pool 90% and gets final product.Through aeration, whisking appliance or underwater propeller sewage is stirred; According to the ammonia nitrogen different concns scope in the sewage and the composite wastewater water yield; The penicillium spp and the sewage that add 0.5-300 ‰ respectively carried out mixed culture 4-8 hour, and penicillium spp both can carry out growth and breeding on as the substratum of unique nitrogenous source at ammonia nitrogen, and can carry out growth and breeding on as the substratum of only nitrogen source at nitrite; After stable; Under the effect of penicillium spp, can realize synchronous nitration and denitrifying function, nitric efficiency reaches as high as 95%, has realized the effect of single stage method biological denitrificaion.This method combines existing bio-denitrifying sewage treatment technology, through adding penicillium spp, changes the processing basis of present biological denitrification process, simplifies technical process, realization synchronous nitration denitrification denitrogenation purpose thereby reach.
Embodiment 1
To the lab scale experimental installation, like sewage quantity 10L/h, ammonia nitrogen concentration 40-50mg/L; The CONTROL PROCESS condition is following: 5 ℃ of temperature, pH value 7, and dissolved oxygen concentration 0.5mg/L stirs sewage through aeration system; The penicillium spp that adds 5ml then mixed, cultivates with sewage 4 hours, and penicillium spp both can carry out growth and breeding on as the substratum of unique nitrogenous source at ammonia nitrogen, and can carry out growth and breeding on as the substratum of only nitrogen source at nitrite; After stable; Under the effect of penicillium spp, can realize synchronous nitration and denitrifying function, nitric efficiency reaches as high as 95%, has realized the effect of single stage method biological denitrificaion.
Embodiment 2
To the sequencing batch active sludge sewage treatment experimental device, like sewage quantity 38L/h, ammonia nitrogen concentration 80-100mg/L; The CONTROL PROCESS condition is following: 20 ℃ of temperature, pH value 7; Dissolved oxygen concentration 4mg/L stirs sewage through whisking appliance, and the penicillium spp that adds 30ml then mixed, cultivates with sewage 5 hours; Penicillium spp both can carry out growth and breeding on as the substratum of unique nitrogenous source at ammonia nitrogen; And can carry out growth and breeding on as the substratum of only nitrogen source at nitrite, stable after, under the effect of penicillium spp, can realize synchronous nitration and denitrifying function; Nitric efficiency reaches as high as 95%, has realized the effect of single stage method biological denitrificaion.
Embodiment 3
To the sequencing batch active sludge sewage treatment experimental device, like sewage quantity 40L/h, ammonia nitrogen concentration 80-100mg/L; The CONTROL PROCESS condition is following: 20 ℃ of temperature, pH value 5; Dissolved oxygen concentration 4mg/L stirs sewage through whisking appliance, and the penicillium spp that adds 30ml then mixed, cultivates with sewage 6 hours; Penicillium spp both can carry out growth and breeding on as the substratum of unique nitrogenous source at ammonia nitrogen; And can carry out growth and breeding on as the substratum of only nitrogen source at nitrite, stable after, under the effect of penicillium spp, can realize synchronous nitration and denitrifying function; Nitric efficiency reaches as high as 95%, has realized the effect of single stage method biological denitrificaion.
Embodiment 4
To the contact oxidation method sewage treatment experimental device, like 1 ton/hour of sewage quantity, ammonia nitrogen concentration 30-40mg/L; The CONTROL PROCESS condition is following: 25 ℃ of temperature, pH value 7; Dissolved oxygen concentration 4mg/L stirs sewage through underwater propeller, and the penicillium spp that adds 500ml then mixed, cultivates with sewage 5 hours; Penicillium spp both can carry out growth and breeding on as the substratum of unique nitrogenous source at ammonia nitrogen; And can carry out growth and breeding on as the substratum of only nitrogen source at nitrite, stable after, under the effect of penicillium spp, can realize synchronous nitration and denitrifying function; Nitric efficiency reaches as high as 95%, has realized the effect of single stage method biological denitrificaion.
Embodiment 5
To the contact oxidation method sewage treatment experimental device; Like 1 ton/hour of sewage quantity, ammonia nitrogen concentration 30-40mg/L, the CONTROL PROCESS condition is following: 40 ℃ of temperature, pH value 9; Dissolved oxygen concentration 6.5mg/L; Cooperate filling carrier in the sewage, concrete filler can adopt 3 D elastic filler+combined stuffing, is covered with contact-oxidation pool 90% and gets final product.Through underwater propeller sewage is stirred; The penicillium spp that adds 500ml then mixed, cultivates with sewage 8 hours, and penicillium spp both can carry out growth and breeding on as the substratum of unique nitrogenous source at ammonia nitrogen, and can carry out growth and breeding on as the substratum of only nitrogen source at nitrite; After stable; Under the effect of penicillium spp, can realize synchronous nitration and denitrifying function, nitric efficiency reaches as high as 95%, has realized the effect of single stage method biological denitrificaion.
Claims (3)
1. method of utilizing the penicillium spp bacterial strain to carry out sewage water denitrification; It is characterized in that: will contain organic sewage and be controlled under the following processing condition: temperature 5-40 ℃, pH value 5.0-9.0; Dissolved oxygen concentration 0.5-6.5mg/L stirs sewage, according to the ammonia nitrogen different concns scope in the sewage and the composite wastewater water yield; The penicillium spp and the sewage that add 0.5-300 ‰ respectively carried out mixed culture 4-8 hour, under the effect of penicillium spp, realized synchronous nitration and denitrification.
2. the method for utilizing the penicillium spp bacterial strain to carry out sewage water denitrification as claimed in claim 1 is characterized in that: can cooperate filling carrier in the sewage, concrete filler can adopt 3 D elastic filler+combined stuffing, is covered with contact-oxidation pool 90% and gets final product.
3. according to claim 1 or claim 2 the penicillium spp bacterial strain that utilizes carries out the method for sewage water denitrification, it is characterized in that: through aeration system, or whisking appliance, or underwater propeller stirs sewage.
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| CN2010105698702A CN102476865A (en) | 2010-11-26 | 2010-11-26 | Wastewater denitrogenation method by use of penicillium strain |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104195048A (en) * | 2014-03-27 | 2014-12-10 | 太原理工大学 | Heterotrophic-nitrification fungus capable of producing mycelium pellets, and culture method and application thereof |
| CN105540839A (en) * | 2015-12-10 | 2016-05-04 | 郭嘉川 | Denitrification method for sewage |
| CN109607824A (en) * | 2018-12-26 | 2019-04-12 | 南京贝克特环保科技有限公司 | A kind of biological nitrogen fixation processing method of high ammonia nitrogen organic wastewater |
| CN117887593A (en) * | 2024-03-13 | 2024-04-16 | 西安建筑科技大学 | Mixed nutrition type denitrifying bacterium Penicillium sp.N8 and application thereof |
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| WO2002024583A1 (en) * | 2000-09-25 | 2002-03-28 | Söll Holding GmbH | Microbiological culture for triggering microbiological processes in water |
| CN101687673A (en) * | 2007-04-12 | 2010-03-31 | 诺维信生物股份有限公司 | Wastewater treatment |
| CN101709269A (en) * | 2009-11-27 | 2010-05-19 | 三峡大学 | Method for screening strains of penicillium sp. and application thereof in degradation of glyphosate waste water |
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2010
- 2010-11-26 CN CN2010105698702A patent/CN102476865A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002024583A1 (en) * | 2000-09-25 | 2002-03-28 | Söll Holding GmbH | Microbiological culture for triggering microbiological processes in water |
| CN101687673A (en) * | 2007-04-12 | 2010-03-31 | 诺维信生物股份有限公司 | Wastewater treatment |
| CN101709269A (en) * | 2009-11-27 | 2010-05-19 | 三峡大学 | Method for screening strains of penicillium sp. and application thereof in degradation of glyphosate waste water |
Non-Patent Citations (1)
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| 赵中艳: "一株青霉菌异养硝化和好氧反硝化特性的研究", 《水资源保护》, vol. 24, no. 2, 30 March 2008 (2008-03-30), pages 74 - 2 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104195048A (en) * | 2014-03-27 | 2014-12-10 | 太原理工大学 | Heterotrophic-nitrification fungus capable of producing mycelium pellets, and culture method and application thereof |
| CN105540839A (en) * | 2015-12-10 | 2016-05-04 | 郭嘉川 | Denitrification method for sewage |
| CN105540839B (en) * | 2015-12-10 | 2018-05-22 | 温岭市创嘉信息科技有限公司 | A kind of method of sewage water denitrification |
| CN109607824A (en) * | 2018-12-26 | 2019-04-12 | 南京贝克特环保科技有限公司 | A kind of biological nitrogen fixation processing method of high ammonia nitrogen organic wastewater |
| CN117887593A (en) * | 2024-03-13 | 2024-04-16 | 西安建筑科技大学 | Mixed nutrition type denitrifying bacterium Penicillium sp.N8 and application thereof |
| CN117887593B (en) * | 2024-03-13 | 2024-05-31 | 西安建筑科技大学 | Mixed nutrition type denitrifying bacterium Penicillium sp.N8 and application thereof |
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Application publication date: 20120530 |