CN109596808A - Impact analysis method of the biogas slurry different amounts to Cd in soil - Google Patents
Impact analysis method of the biogas slurry different amounts to Cd in soil Download PDFInfo
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- CN109596808A CN109596808A CN201811486229.5A CN201811486229A CN109596808A CN 109596808 A CN109596808 A CN 109596808A CN 201811486229 A CN201811486229 A CN 201811486229A CN 109596808 A CN109596808 A CN 109596808A
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- 239000002689 soil Substances 0.000 title claims abstract description 104
- 239000002002 slurry Substances 0.000 title claims abstract description 94
- 238000004458 analytical method Methods 0.000 title claims abstract description 28
- 229910001385 heavy metal Inorganic materials 0.000 claims abstract description 34
- 241001122767 Theaceae Species 0.000 claims abstract description 30
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 claims abstract description 15
- 239000000463 material Substances 0.000 claims abstract description 7
- 239000000126 substance Substances 0.000 claims abstract description 7
- 239000002420 orchard Substances 0.000 claims abstract description 3
- 238000012545 processing Methods 0.000 claims description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 238000002474 experimental method Methods 0.000 claims description 9
- 238000000605 extraction Methods 0.000 claims description 9
- 238000001179 sorption measurement Methods 0.000 claims description 9
- 229910052793 cadmium Inorganic materials 0.000 claims description 8
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 claims description 8
- 241001465754 Metazoa Species 0.000 claims description 7
- 241000196324 Embryophyta Species 0.000 claims description 5
- 230000002262 irrigation Effects 0.000 claims description 5
- 238000003973 irrigation Methods 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- 238000010521 absorption reaction Methods 0.000 claims description 4
- 238000012925 biological evaluation Methods 0.000 claims description 4
- 238000007796 conventional method Methods 0.000 claims description 4
- 238000007405 data analysis Methods 0.000 claims description 4
- 239000008367 deionised water Substances 0.000 claims description 4
- 229910021641 deionized water Inorganic materials 0.000 claims description 4
- 229910002804 graphite Inorganic materials 0.000 claims description 4
- 239000010439 graphite Substances 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 4
- 230000000877 morphologic effect Effects 0.000 claims description 4
- 238000005070 sampling Methods 0.000 claims description 4
- 238000000354 decomposition reaction Methods 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 abstract description 16
- 239000005416 organic matter Substances 0.000 abstract description 14
- 230000000694 effects Effects 0.000 abstract description 8
- 238000005342 ion exchange Methods 0.000 abstract description 8
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N iron oxide Inorganic materials [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 abstract description 7
- AMWRITDGCCNYAT-UHFFFAOYSA-L manganese oxide Inorganic materials [Mn].O[Mn]=O.O[Mn]=O AMWRITDGCCNYAT-UHFFFAOYSA-L 0.000 abstract description 7
- PPNAOCWZXJOHFK-UHFFFAOYSA-N manganese(2+);oxygen(2-) Chemical class [O-2].[Mn+2] PPNAOCWZXJOHFK-UHFFFAOYSA-N 0.000 abstract description 7
- 239000002253 acid Substances 0.000 abstract description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000010871 livestock manure Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 229910002551 Fe-Mn Inorganic materials 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 238000005904 alkaline hydrolysis reaction Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000003621 irrigation water Substances 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000033116 oxidation-reduction process Effects 0.000 description 1
- 150000002927 oxygen compounds Chemical class 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000012502 risk assessment Methods 0.000 description 1
- 238000004856 soil analysis Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/24—Earth materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/71—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light thermally excited
- G01N21/74—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light thermally excited using flameless atomising, e.g. graphite furnaces
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/24—Earth materials
- G01N2033/245—Earth materials for agricultural purposes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/24—Earth materials
- G01N2033/248—Earth materials related to manure as a biological product, i.e. excluding artificial fertilizers
Abstract
The invention belongs to soil surveying technical field, a kind of biogas slurry different amounts are disclosed to the impact analysis method of Cd form in soil, to pour the soil of biogas slurry for a long time as material, analysis biogas slurry pours the influence to the form and biological effectiveness of heavy metal Cd in Orchard Soil.Biogas slurry is applied in tea garden soil of the present invention can inhibit the bioactivity and biological effectiveness of heavy metal Cd, and the ion-exchange state of Cd, iron and manganese oxides reference state, carbonate combine state are converted to organic matter reference state and residual form in soil.The biological unavailability of Cd is significantly improved, improves 5.14%~31.64%；The bioactivity and biological effectiveness of Cd are reduced, the bioactivity of Cd reduces by 11.63%~50.14%；Biogas slurry has preferable effect in terms of improving soil physical and chemical property；Be conducive to analysis biogas slurry to develop and use on the acid soils such as tea place, reduce the pollution of tea garden soil, improves the safety of tealeaves.
Description
Technical field
A kind of influence point the invention belongs to soil surveying technical field more particularly to biogas slurry different amounts to Cd in soil
Analysis method.
Background technique
Currently, the prior art commonly used in the trade is such that
Contain the nutrients such as a large amount of N, P, K, micronutrient element, amino acid, enzyme in biogas slurry after livestock manure fermented
Matter is rationally conducive to improve soil texture using biogas slurry, alleviates soil ph, and preservation of fertility promotes soil ecology ring
Border benign cycle, can reduce the generation of crop disease and insect, improve the yield and quality of agricultural product.Biogas slurry is liquid fertilizer, agricultural
After can reduce agricultural irrigation water, improve water resource utilization efficiency.
But in recent years, animal husbandry adds the elements such as heavy metal in animal and fowl fodder, leads to animal dung for higher profit
Just middle contents of heavy metal elements increases, by containing heavy metal element in the product biogas slurry after livestock manure fermented.The agricultural mistake of biogas slurry
It may cause heavy metal pollution of soil in journey.Therefore, the agricultural health risk assessment of biogas slurry has been a concern.
The life activity of existing heavy metals in soil has outside the Pass in addition to the content with heavy metal in soil, also and with much money
The existing forms belonged in the soil have much relations, and form existing for heavy metal in soil is different, and bioactivity is different.With much money
Belong to total amount to be difficult to reflect the bioactivity of heavy metal, and existing form can effectively evaluate its chemistry to heavy metal in the soil
Behavior.
Under different soil environment conditions, such as the change of soil pH, content of organic matter factor can cause soil
The conversion of middle heavy metal existing forms, the validity of the heavy metal on plants of different shape are different.On the acid soil of tea place
After applying biogas slurry, the pH value and the content of organic matter of soil change.
Currently, it is more in relation to the research that compost, bio-fertilizer are applied in tea place, and about biogas slurry to tea growth situation, production
Amount and the influence Research on differences of safety are rarely reported.Tea garden soil slant acidity has an impact to soil acidity or alkalinity after applying biogas slurry,
Have an impact to the existing forms of heavy metal Cd in the soil.
In conclusion problem of the existing technology is:
(1) existing biogas slurry is indefinite to tea growth situation, yield and the influence of safety variance analysis；
(2) have an impact after applying biogas slurry to soil acidity or alkalinity, biogas slurry is continuous due to tea garden soil slant acidity in the prior art
Pouring processing, reduces the biological unavailability of Cd；The bioactivity and biological effectiveness of Cd are improved, tea garden soil, drop are polluted
The safety of low tealeaves.
Solve the difficulty and meaning of above-mentioned technical problem:
The present invention analyzes biogas slurry pouring pair using indoor culture experiment using the tea garden soil for pouring biogas slurry for a long time as material
The influence of the form and biological effectiveness of heavy metal Cd in tea garden soil is commented for biogas slurry in the resource utilization in tea place and safety
Offer reference is provided.
Summary of the invention
In view of the problems of the existing technology, the influence the present invention provides a kind of biogas slurry different amounts to Cd in soil point
Analysis method.
To pour the soil of biogas slurry for a long time as material；It is poured using indoor culture experiment analysis biogas slurry to a huge sum of money in Orchard Soil
Belong to the influence of the form and biological effectiveness of Cd, and carries out the assessment of biogas slurry safe utilization.To be improve tea yield, quality
And safety provides effective way, and provides foundation rationally to reduce tea place ecological risk using organic fertilizers such as biogas slurries.
The invention is realized in this way a kind of impact analysis method of biogas slurry different amounts to Cd in soil, the biogas slurry
Different amounts to the impact analysis method of Cd in soil, specifically includes the following steps:
Step 1: acquisition is taken tea place trial zone arable layer in continuous administration biogas slurry 4 years, is removed visible for trying soil
Undecomposed and half decomposition plant and animal residues and biggish grit, it is spare；
Step 2: according to overall control principle, set 4 processing according to tealeaves irrigation volume, CK: clear water compares (CK)；T1: application
0.5N(NH4 +- N) biogas slurry；T2: application 1N (NH4 +- N) biogas slurry；T3: application 2N (NH4 +- N) biogas slurry；
Step 3: using indoor culture experiment, claim 500g fresh soil sample to be put into 1000mL vial and cultivate, be separately added into
The biogas slurry of same volume keeps field capacity 70%, cultivates 60 days；
Step 4: Soil K+adsorption measures soil physical and chemical property using conventional method, is divided light using sampling Graphite Furnace Atomic Absorption
Degree meter measures effective cadmium and full cadmium content；
Step 5: data processing is arranged using Excel2010 software, carries out data analysis, place using SPSS16.0 software
Reason, statistics.
Further, in step 1, the arable layer of tea place trial zone, depth 0-20cm.
Further, in step 2,3 repetitions are done in each processing.
Further, in step 4, Soil K+adsorption is accurately weighed respectively using Tessier continuous extraction for trying soil
(2.00 ± 0.01) g is extracted according to Tessier continuous extraction program；Specific steps are as follows:
(1) extracting solution of each form is centrifuged 15min with 4000r/min, crosses 0.45um filter membrane, to be measured；
(2) after the completion of each step extraction process, residue is eluted 2 times with 10mL deionized water；
(3) residue after will be washed is used for the morphological analysis of next step；Simultaneously set one group it is parallel.
Further, in step 4, Soil K+adsorption, biological evaluation, the bioactivity of heavy metal in soil includes biology
Utilizability, the potential usability of biology, biology hardly possible usability, wherein bioavailability availability coefficient K is described:
K=(commutative friendship+carbonate combine state)/full dose=(F1+F2)/(F1+F2+F3+F4+F5)
F1, F2, F3, F4, F5 are respectively 5 kinds of forms of heavy metal-polluted soil in formula.
In conclusion advantages of the present invention and good effect are as follows:
Compared with the control, the processing of biogas slurry continuous pouring significantly improves the biological unavailability of Cd to the present invention, improves
5.14%~31.64%.Reducing the bioactivity and biological effectiveness of Cd, the bioactivity of Cd reduces by 11.63%~
50.14%.
The present invention the result shows that, biogas slurry has preferable effect in terms of improving soil physical and chemical property.
Present invention demonstrates that the bioactivity and biological effectiveness of heavy metal Cd, soil can be inhibited by applying biogas slurry in tea garden soil
The ion-exchange state of middle Cd, iron and manganese oxides reference state, carbonate combine state are converted to organic matter reference state and residual form.Cause
This, can further analyze biogas slurry and develop and use on the acid soils such as tea place, reduce the pollution of tea garden soil, improve tealeaves
Safety.
Detailed description of the invention
Fig. 1 is impact analysis method flow chart of the biogas slurry different amounts provided in an embodiment of the present invention to Cd in soil.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
The present invention analyzes biogas slurry pouring pair using indoor culture experiment using the tea garden soil for pouring biogas slurry for a long time as material
The influence of the form and biological effectiveness of heavy metal Cd in tea garden soil is commented for biogas slurry in the resource utilization in tea place and safety
Offer reference is provided；And carry out the assessment of biogas slurry safe utilization.
Application principle of the invention is further elaborated with reference to the accompanying drawing；
As shown in Figure 1, impact analysis method of the biogas slurry different amounts provided in an embodiment of the present invention to Cd in soil, specifically
The following steps are included:
S101: acquisition takes tea place trial zone arable layer in continuous administration biogas slurry 4 years, removal is visible not for trying soil
The plant and animal residues and biggish grit for decomposing and partly decomposing, it is spare；
S102: according to overall control principle, set 4 processing according to tealeaves irrigation volume, CK: clear water compares (CK)；T1: application
0.5N(NH4 +- N) biogas slurry；T2: application 1N (NH4 +- N) biogas slurry；T3: application 2N (NH4 +- N) biogas slurry；
S103: using indoor culture experiment, claim 500g fresh soil sample to be put into 1000mL vial and cultivate, be separately added into phase
The biogas slurry of same volume keeps field capacity 70%, cultivates 60 days；
S104: Soil K+adsorption measures soil physical and chemical property using conventional method, using sampling Graphite Furnace Atomic Absorption spectrophotometric
Meter measures effective cadmium and full cadmium content；
S105: data processing is arranged using Excel2010 software, carries out data analysis, place using SPSS16.0 software
Reason, statistics.
In step S101, tea place trial zone provided in an embodiment of the present invention arable layer, depth 0-20cm.
In step S102,3 repetitions are done in each processing provided in an embodiment of the present invention.
In step S104, Soil K+adsorption provided in an embodiment of the present invention claims using Tessier continuous extraction is accurate respectively
It takes for trying soil (2.00 ± 0.01) g, is extracted according to Tessier continuous extraction program；Specific steps are as follows:
(1) extracting solution of each form is centrifuged 15min with 4000r/min, crosses 0.45um filter membrane, to be measured；
(2) after the completion of each step extraction process, residue is eluted 2 times with 10mL deionized water；
(3) residue after will be washed is used for the morphological analysis of next step；Simultaneously set one group it is parallel.
In step S104, Soil K+adsorption provided in an embodiment of the present invention, biological evaluation, the biology of heavy metal in soil
Activity includes bioavailability, the potential usability of biology, and biology hardly possible usability, wherein bioavailability availability coefficient K comes
Description:
K=(commutative friendship+carbonate combine state)/full dose=(F1+F2)/(F1+F2+F3+F4+F5)
F1, F2, F3, F4, F5 are respectively 5 kinds of forms of heavy metal-polluted soil in formula.
Below with reference to specific test, the invention will be further described.
Experiment 1；
1 materials and methods
(1) test material
Peasant household, Songyang County inferior lobe village methane-generating pit is derived from for examination biogas slurry.The village Ling Sixia, the Songyang County township Ye Cun is picked up from for examination soil to connect
The tea place trial zone arable layer of continuous application biogas slurry 4 years, depth 0-20cm remove animals and plants that are visible undecomposed and partly decomposing
Residuum and biggish grit, it is spare.The basic physicochemical character of soil are as follows: content of organic matter 22.08gkg-1, alkaline hydrolysis nitrogen content
63.76mg·kg-1, available phosphorus content 11.32mgkg-1, quick-acting potassium content 132.10mgkg-1, pH value 5.05.
(2) test method
Nitrogen mainly exists in the form of ammonia nitrogen in biogas slurry, therefore the biogas slurry irrigation volume respectively handled is rolled over by ammonia-nitrogen content
It calculates.According to local tealeaves NH4 +- N applies standard, with taken biogas slurry NH4 +- N concentration conversion tea place amount of application.According to overall control
Principle sets 4 processing according to tealeaves irrigation volume, CK: clear water compares (CK)；T1: application 0.5N (NH4 +- N) biogas slurry；T2: application 1N
(NH4 +- N) biogas slurry；T3: application 2N (NH4 +- N) biogas slurry.3 repetitions of each processing.Using indoor culture experiment, claim 500g fresh
Soil sample is put into 1000mL vial and cultivates, and is separately added into the biogas slurry of same volume, keeps field capacity 70%, culture 60
It.
(3) analysis method
Soil physical and chemical property is measured using conventional method, and effective cadmium and full cadmium content are using sampling Graphite Furnace Atomic Absorption light splitting light
Degree meter measurement.
1) Tessier continuous extraction is accurately weighed respectively for trying soil (2.00 ± 0.01) g, continuous according to Tessier
Extraction method program (table 1) extracts.The extracting solution of each form is centrifuged 15min with 4000r/min, crosses 0.45um filter membrane
It is to be measured.After the completion of each step extraction process, residue with 10mL deionized water elute 2 times, then will be washed after residue
Morphological analysis for next step.Simultaneously set one group it is parallel.
1 Tessier continuous extraction of table
2) bioactivity of biological evaluation heavy metal in soil includes bioavailability, the potential usability of biology,
Biological hardly possible usability, wherein bioavailability availability coefficient K is described:
F1, F2, F3, F4, F5 are respectively 5 kinds of forms of heavy metal-polluted soil in formula.
K=(commutative friendship+carbonate combine state)/full dose=(F1+F2)/(F1+F2+F3+F4+F5)
(4) data processing method
It is arranged using Excel2010 software, data analysis, processing, statistics is carried out using SPSS16.0 software.
2 results and analysis
(1) influence of the biogas slurry continuous pouring to heavy metal Cd form content
Each form content (mgkg of heavy metal Cd under 2 biogas slurry continuous pouring different disposal of table-1)
The processing of biogas slurry continuous pouring increases the ion-exchange state of Cd in soil, residual form amount, and with biogas slurry dosage
Increase and increases；Reduce the carbonate combine state of Cd in soil, iron and manganese oxides reference state, organic matter reference state amount, wherein
Carbonate combine state, iron and manganese oxides reference state content are reduced with the increase of biogas slurry dosage, organic matter reference state amount with
The increase of biogas slurry dosage and increase.Wherein, significant difference between the ion-exchange state of Cd, organic matter reference state are respectively handled in soil；
In soil the carbonate combine state content of Cd between T2 and T3 processing without notable difference, significant difference between other processing；Ferrimanganic
Oxidizable, residual form content are respectively between CK and T1 processing without notable difference, significant difference between other processing.
(2) biogas slurry continuous pouring processing heavy metal Cd morphosis analysis
Each fractions distribution of heavy metal Cd (%) under 3 biogas slurry continuous pouring different disposal of table
Each ion-exchange state, carbonate combine state, iron and manganese oxides reference state, organic matter for handling lower heavy metal Cd combines
State, residual form distribution are shown in Table 3.Each form of the lower heavy metal Cd of CK, T1 processing is ordered as residual form > carbonate by proportion
Reference state > organic matter reference state > iron and manganese oxides reference state > ion-exchange state, wherein residual form proportion is maximum.T2,
Each form of Cd is ordered as residual form > organic matter reference state > carbonate combine state > iron by proportion in T3 processing soil
Mn oxide reference state > ion-exchange state, wherein residual form proportion is maximum.
(3) influence of the biogas slurry continuous pouring to heavy metal Cd bioactivity and bioavailability
The bioactivity and biological effectiveness of heavy metal Cd under 4 biogas slurry continuous pouring different disposal of table
The heavy metal of exchangeable species is most easily bioavailable, and toxicity is most strong, carbonate combine state be also easier to discharge again into
Enter water phase, Fe-Mn oxide can also be discharged when oxidation reduction potential value changes, and have likely effectiveness, organic matter knot to biology
State is closed then to be not easy to be absorbed, and the combination of residual form and deposit is most secured, activity is minimum, thus toxicity is minimum, inanimate object effect
It answers.Each bioactivity for handling lower heavy metal and biological effectiveness are listed in table 4 accordingly.
As shown in Table 4, compared with the control, the processing of biogas slurry continuous pouring is remarkably improved the biological unavailability of Cd, mentions
It is high by 5.14%~31.64%.Reducing the bioactivity and biological effectiveness of Cd, the bioactivity of Cd reduces by 11.63%~
50.14%；The potential usability of biology of Cd shows as the trend fallen after rising, and the potential usability of biology of T1 processing, Cd is higher than
CK processing, the potential usability of biology that T2, T3 handle Cd reduce；As biogas slurry amount of application increases, the potential usability of biology of Cd
In decreasing trend.
3 results
Biogas slurry can change the form of heavy metal in soil Cd, and the ion that the processing of biogas slurry continuous pouring increases Cd in soil is handed over
State, residual form amount are changed, and is increased with the increase of biogas slurry dosage；Reduce the carbonate combine state of Cd in soil, ferrimanganic oxygen
Compound reference state, organic matter reference state amount.
Compared with the control, biogas slurry continuous pouring processing significantly improves the biological unavailability of Cd, improve 5.14%~
31.64%.The bioactivity and biological effectiveness of Cd are reduced, the bioactivity of Cd reduces by 11.63%~50.14%.
Biogas slurry has preferable effect in terms of improving soil physical and chemical property.
Present invention demonstrates that the bioactivity and biological effectiveness of heavy metal Cd, soil can be inhibited by applying biogas slurry in tea garden soil
The ion-exchange state of middle Cd, iron and manganese oxides reference state, carbonate combine state are converted to organic matter reference state and residual form.Cause
This, can further analyze biogas slurry and develop and use on the acid soils such as tea place.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (6)
1. a kind of biogas slurry different amounts are to the impact analysis method of Cd in soil, which is characterized in that the biogas slurry different amounts pair
The impact analysis method of Cd to pour the soil of biogas slurry as material for a long time in soil；It is poured using indoor culture experiment analysis biogas slurry
Influence to the form and biological effectiveness of heavy metal Cd in Orchard Soil, and carry out the assessment of biogas slurry safe utilization.
2. biogas slurry different amounts as described in claim 1 are to the impact analysis method of Cd in soil, which is characterized in that the natural pond
Liquid different amounts to the impact analysis method of Cd in soil, specifically includes the following steps:
Step 1: acquisition takes tea place trial zone arable layer in continuous administration biogas slurry 4 years, removal is visible not to divide for trying soil
The plant and animal residues and biggish grit of solution and half decomposition, it is spare；
Step 2: according to overall control principle, set 4 processing according to tealeaves irrigation volume, CK: clear water compares (CK)；T1: application 0.5N
(NH4 +- N) biogas slurry；T2: application 1N (NH4 +- N) biogas slurry；T3: application 2N (NH4 +- N) biogas slurry；
Step 3: using indoor culture experiment, claim 500g fresh soil sample to be put into 1000mL vial and cultivate, be separately added into identical
The biogas slurry of volume keeps field capacity 70%, cultivates 60 days；
Step 4: Soil K+adsorption measures soil physical and chemical property using conventional method, using sampling Graphite Furnace Atomic Absorption spectrophotometer
Measure effective cadmium and full cadmium content；
Step 5: data processing, using Excel2010 software arrange, using SPSS16.0 software carry out data analysis, processing,
Statistics.
3. biogas slurry different amounts as claimed in claim 2 are to the impact analysis method of Cd in soil, which is characterized in that the step
In rapid one, the arable layer of tea place trial zone, depth 0-20cm.
4. biogas slurry different amounts as claimed in claim 2 are to the impact analysis method of Cd in soil, which is characterized in that the step
In rapid two, 3 repetitions are done in each processing.
5. biogas slurry different amounts as claimed in claim 2 are to the impact analysis method of Cd in soil, which is characterized in that the step
In rapid four, Soil K+adsorption, using Tessier continuous extraction accurately weigh respectively for try soil (2.00 ± 0.01) g, according to
Tessier continuous extraction program extracts；Specific steps are as follows:
(1) extracting solution of each form is centrifuged 15min with 4000r/min, crosses 0.45um filter membrane, to be measured；
(2) after the completion of each step extraction process, residue is eluted 2 times with 10mL deionized water；
(3) residue after will be washed is used for the morphological analysis of next step；Simultaneously set one group it is parallel.
6. biogas slurry different amounts as claimed in claim 2 are to the impact analysis method of Cd in soil, which is characterized in that the step
In rapid four, Soil K+adsorption, biological evaluation, the bioactivity of heavy metal in soil include bioavailability, biology it is potential
Usability, biology hardly possible usability, wherein bioavailability availability coefficient K is described:
K=(commutative friendship+carbonate combine state)/full dose=(F1+F2)/(F1+F2+F3+F4+F5)
F1, F2, F3, F4, F5 are respectively 5 kinds of forms of heavy metal-polluted soil in formula.
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| CN110333340A (en) * | 2019-07-31 | 2019-10-15 | 招商局生态环保科技有限公司 | The analysis method of Cr VI form in a kind of improved soil |
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| CN115647028A (en) * | 2022-11-15 | 2023-01-31 | 中国长江三峡集团有限公司 | Method for restoring lead-polluted soil of acid pyrite mine |
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