CN109593653B - Biological cell culture device - Google Patents
Biological cell culture device Download PDFInfo
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- CN109593653B CN109593653B CN201811558913.XA CN201811558913A CN109593653B CN 109593653 B CN109593653 B CN 109593653B CN 201811558913 A CN201811558913 A CN 201811558913A CN 109593653 B CN109593653 B CN 109593653B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
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Abstract
The invention relates to a biological cell culture device, which relates to the technical field of biological research.A driving motor is fixedly arranged in a box body on the right side of a base, and a driving gear is fixedly arranged on the output end of the driving motor; a driven gear is fixedly sleeved on the outer side wall of the support column and is connected with the driving gear through a chain; an observation table is fixedly inserted on the side wall of the box body on the right side of the operation table, the right side of the observation table extends to the right side of the box body, and a glass cover is fixedly covered on the extending end; a water storage tank is fixedly arranged at the left side of the tank body; the inside of storage water tank is fixed and is provided with the suction pump, and the output fixedly connected with house steward of suction pump, house steward's upper end pass the upper portion of storage water tank after, are connected with shower and circulating pipe respectively through three way connection, possess the ability of adjusting indoor temperature and humidity, have improved the cultivation efficiency of cell greatly, have improved the survival rate of cell, and when needs are observed, can make things convenient for the experimenter to observe and study.
Description
Technical Field
The invention relates to the technical field of biological research, in particular to a biological cell culture device.
Background
The biological cells are stem cells, which are simply primitive undifferentiated cells having a multi-directional differentiation potential and a self-replicating ability, and are primitive cells forming various tissues and organs of mammals. The stem cells have common morphology, are usually circular or oval, have small cell volume and relatively large nuclei, mostly have euchromatin and have high telomerase activity.
At present, a biological cell culture chamber does not have the capacity of adjusting indoor temperature and humidity, the cell culture efficiency is low, so that the cell survival rate is relatively low, the cell culture chamber needs to be taken out for observation when being observed, the observation and the research are inconvenient, and the improvement is needed to be needed.
Disclosure of Invention
The invention aims to overcome the defects and shortcomings of the prior art, provides the biological cell culture device which is simple in structure, reasonable in design and convenient to use, has the capability of adjusting indoor temperature and humidity, greatly improves the cell culture efficiency, improves the cell survival rate, and can facilitate observation and research of experimenters when observation is needed.
In order to achieve the purpose, the invention adopts the technical scheme that: it comprises a box body, an operation platform and a culture dish; an operation table is arranged in the box body, and a plurality of culture dishes are arranged on the operation table in a round angle manner; the device also comprises a driving motor, a water storage tank, an electric cabinet, an observation platform and a glass cover; a base is vertically and fixedly arranged at the inner bottom of the box body, a support column is vertically and fixedly arranged at the bottom of the operating platform, and the upper part of the base is fixedly connected with the bottom of the support column through a flat-top thrust ball bearing; a driving motor is fixedly arranged in the box body on the right side of the base, and a driving gear is fixedly arranged on the output end of the driving motor; a driven gear is fixedly sleeved on the outer side wall of the support column and is connected with the driving gear through a chain; a shaft post is vertically and fixedly arranged at the inner bottom of the box body, and the shaft post is rotatably arranged in the middle of the operating platform, the supporting column and the base in a penetrating manner through a bearing; the upper end of the shaft column is fixedly provided with a fixed seat, and a push rod is fixedly arranged on the fixed seat; a magnet is fixedly arranged on the end head of the push rod; a plurality of right angle seats are fixedly arranged on the operating platform, every two right angle seats are oppositely arranged, and the culture dish is arranged between the two opposite right angle seats; an iron block is fixedly arranged on the side wall of the culture dish, which is attached to the two right-angle seats, and the iron block and the magnet are arranged in an attractive manner; an observation table is fixedly inserted into the side wall of the box body on the right side of the operation table, the height of the left side of the observation table is flush with the height of the operation table, the right side of the observation table extends to the right side of the box body, and a glass cover is fixedly covered on the extending end of the observation table; the bottom of the glass cover is fixed on the observation platform, and the left side of the glass cover is fixed on the side wall of the box body; a water storage tank is fixedly arranged on the left side of the tank body, and a water inlet and outlet is fixedly arranged at the bottom of the left side wall of the water storage tank; a water suction pump is fixedly arranged inside the water storage tank, the output end of the water suction pump is fixedly connected with a main pipe, the upper end of the main pipe penetrates through the upper part of the water storage tank and is respectively connected with a spray pipe and a circulating pipe through a three-way joint, and control valves are fixedly arranged on the spray pipe and the circulating pipe; the right end of the spray pipe penetrates through the left side wall of the box body and is communicated with the connecting pipe; the connecting pipe is fixedly arranged on the left side wall in the box body through a pipe clamp; a plurality of atomizing nozzles are fixedly arranged on the connecting pipe, and the atomizing nozzles extend to the upper part of the operating platform; the right end of the circulating pipe penetrates through the left side wall of the box body and is fixedly arranged on the rear side wall in the box body in a U shape; a plurality of first heating pipes are fixedly arranged inside the water storage tank; an electric cabinet is fixedly arranged on the upper portion of the left side wall of the box body, and the driving motor, the push rod and the first heating pipe are all connected with a power supply in the electric cabinet.
Furthermore, a drying box is fixedly arranged at the top of the box body; the top of the box body is fixedly provided with a plurality of vent holes; a suction pump is fixedly arranged at the top of the drying box, and the input end of the suction pump is communicated with the inside of the drying box; a second heating pipe is fixedly arranged inside the drying box, and the suction pump and the second heating pipe are both connected with a power supply in the electric cabinet; a net cage is fixedly arranged at the bottom of the drying box, a screen plate is fixedly arranged in the net cage, activated carbon is fixedly filled in the net cage at the upper part of the screen plate, and calcium oxide particles are fixedly filled in the net cage at the bottom of the screen plate; the suction pump sucks the air in the box body into the drying box for drying, the second heating pipe dries the air through a heating method, the activated carbon filters impurities in the air, and the calcium oxide particles adsorb moisture in the air.
Furthermore, a supporting table is fixedly arranged on the inner wall of the left side of the box body, and a guide wheel is rotatably arranged on the supporting table through a wheel seat; a circular guide rail is fixedly arranged at the bottom of the operating platform, and the guide wheel is arranged on the circular guide rail in a rolling manner; the support table supports the left side of the operating table, and the guide wheels roll on the circular guide rails.
Furthermore, the operating platform is fixedly provided with a temperature sensor probe, the temperature sensor probe is connected with a power supply in the electric cabinet, the temperature sensor probe detects the temperature condition in the box body, and then data are transmitted to a controller in the electric cabinet.
Furthermore, a high liquid level sensor is fixedly arranged on the left side wall of the top in the water storage tank, a low liquid level sensor is fixedly arranged on the left side wall of the bottom in the water storage tank, and the high liquid level sensor and the low liquid level sensor are both connected with a power supply in the electric cabinet; the high liquid level sensor and the low liquid level sensor are used for detecting the liquid level condition in the water storage tank and then transmitting data to the controller in the electric cabinet.
Furthermore, a three-color warning lamp is fixedly arranged at the top of the water storage tank and is connected with a power supply in the electric cabinet; the three-color warning light is used for prompting the liquid level condition in the water storage tank.
Furthermore, an angle adjusting device is fixedly arranged on the right side wall inside the box body, a lamp holder is fixedly arranged at the left end of the angle adjusting device, a lamp holder is fixedly clamped on the lamp holder, and the lamp holder is connected with a power supply in the electric cabinet; the angle adjusting device comprises a first connecting rod, a second connecting rod, a first disc, a second disc and a fastening hand; the left end of the first connecting rod is fixedly connected with the lamp holder, and the right end of the first connecting rod is fixedly connected with the first disc; the right end of the second connecting rod is fixedly arranged on the right side wall of the box body, the left end of the second connecting rod is fixedly connected with a second disc, and the first disc and the second disc are arranged oppositely; racks are fixedly arranged on the opposite surfaces of the first disc and the second disc, and the two racks are clamped; the middle parts of the first disc and the second disc are fixedly connected through tightening hand screwing; the angle between a regulation connecting rod and No. two connecting rods to can adjust the angle of shining of lamp holder, loosen the fastening hand and twist, adjust the position of rack toothing on a disc and No. two disc opposite faces, adjust the angle between a connecting rod and No. two connecting rods.
The working principle of the invention is as follows: opening a box door of the box body, respectively placing culture dishes in which culture media and biological cells are placed between every two right-angle seats, driving a driving motor to drive a driving gear to rotate, and driving the driving gear and a driven gear to be connected through a chain so that the driven gear starts to rotate, and then driving an operating platform on a support column to start to rotate around a shaft column; when the culture dish needs to be observed, the driving motor is started, the culture dish needing to be observed is stopped at the right end of the push rod, then the driving motor is suspended, the push rod is started, the push rod extends, the magnet and the iron block are attracted with each other, then the culture dish can be pushed onto an observation platform, and an experimenter can directly observe the growth condition of biological cells in the culture dish through the glass cover; when the box body needs to be humidified, the control valve on the circulating pipe is closed, and the control valve on the spraying pipe is opened, so that water in the water storage tank can be pumped into the spraying pipe through the suction pump and then is sprayed out through the atomizing nozzle; when the water needs to be heated, a control valve on the circulating pipe is opened, a control valve on the spray pipe is closed, then a heating pipe in the water storage tank is heated, so that the water temperature in the water storage tank is increased, and then the high-temperature water circulates in the circulating pipe to drive the temperature in the tank body to be slowly increased.
After adopting above-mentioned structure, this embodiment beneficial effect does:
1. the suction pump conveys water in the water storage tank into the spraying pipe from the header pipe, and then the water is sprayed out through the atomizing nozzles to adjust the humidity in the tank, so that the cell culture efficiency can be improved, and the cell survival rate is improved.
2. Magnet and iron plate attraction are drawn to the push rod extension for on the culture dish promoted the observation bench from the operation panel, the laboratory staff can observe biological cell's cultivation situation through the glass cover promptly, makes things convenient for laboratory staff to observe and research.
3. A heating pipe in the storage water tank heats the water source in the storage water tank, and after the heating is completed, hot water is conveyed to the inside of the circulating pipe through the water suction pump, so that the inside of the box body can be heated, and the survival efficiency of cells can be improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
Fig. 1 is a schematic structural view of the present invention.
FIG. 2 is a schematic view of the connection structure of the operation table, the culture dish, the fixing base and the push rod of the present invention.
Fig. 3 is a schematic view of a coupling structure of the guide wheel and the circular guide rail of the present invention.
Fig. 4 is a schematic structural view of the angle adjusting device of the present invention.
Fig. 5 is a schematic view showing a deployed structure of the angle adjusting apparatus of the present invention.
Description of reference numerals:
the device comprises a box body 1, an operation table 2, a culture dish 3, a base 4, a support column 5, a flat-top thrust ball bearing 6, a driving motor 7, a driving gear 8, a driven gear 9, a chain 10, a shaft column 11, a fixed seat 12, a push rod 13, a magnet 14, a right-angle seat 15, an iron block 16, an observation table 17, a glass cover 18, a water storage tank 19, a water inlet and outlet 20, a water suction pump 21, a header pipe 22, a spray pipe 23, a circulating pipe 24, a control valve 25, a connecting pipe 26, an atomizing nozzle 27, a first heating pipe 28, an electric cabinet 29, a drying cabinet 30, a vent hole 31, a suction pump 32, a second heating pipe 33, a net cage 34, a net plate 35, activated carbon 36, calcium oxide particles 37, a support table 38, a guide wheel 39, a circular guide rail 40, a temperature sensor probe 41, a high liquid level sensor 42, a low liquid level sensor 43, a three-color warning lamp 44, an angle adjusting device 45, a first connecting rod 45-1, a second connecting rod 45-2, a third connecting rod, 45-3 parts of a first disk, 45-4 parts of a second disk, 45-5 parts of a fastening hand, 45-6 parts of a rack, 46 parts of a lamp holder and 47 parts of a lamp holder.
Detailed Description
The invention will be further described with reference to the accompanying drawings.
Referring to fig. 1 to 5, the technical solution adopted by the present embodiment is: it comprises a box body 1, an operation platform 2 and a culture dish 3; an operation table 2 is arranged in the box body 1, a plurality of culture dishes 3 are arranged on the operation table 2 at equal round angles, the culture dishes 3 are square culture dishes and are convenient to fix, the box body 1 is a cell culture box in the prior art, and the cell culture box is provided with a sealing box door and a hasp and the like, and is the prior art; the device also comprises a driving motor 7, a water storage tank 19, an electric cabinet 29, an observation platform 17 and a glass cover 18; a base 4 is vertically and fixedly welded at the inner bottom of the box body 1, a support pillar 5 is vertically and fixedly welded at the bottom of the operating platform 2, the upper part of the base 4 is fixedly connected with the bottom of the support pillar 5 through a flat-top thrust ball bearing 6, and the operating platform 2 can conveniently rotate around the base 4; a driving motor 7 is fixedly arranged in the box body 1 on the right side of the base 4, the type of the driving motor 7 is 60KTYZ, and a driving gear 8 is fixedly welded on the output end of the driving motor 7; a driven gear 9 is fixedly sleeved on the outer side wall of the support pillar 5, the driven gear 9 is connected with the driving gear 8 through a chain 10, and the driving motor 7 can drive the support pillar 5 to rotate through the connected driving gear 8 and the driven gear 9; a shaft post 11 is vertically and fixedly welded at the inner bottom of the box body 1, and the shaft post 11 is rotatably arranged in the middle of the operating platform 2, the support column 5 and the base 4 in a penetrating manner through a bearing; the upper end of the shaft column 11 is fixedly provided with a fixed seat 12, a push rod 13 is fixedly arranged on the fixed seat 12, the push rod 13 is a single-leg three-section electric push rod, and the model of the push rod is SMR-300; the end of the push rod 13 is fixedly provided with a magnet 14 through a bolt; 12 right-angle seats 15 are fixedly arranged on the operating platform 2, every two right-angle seats 15 are oppositely arranged, and the culture dish 3 is arranged between the two opposite right-angle seats 15, so that the culture dish 3 is conveniently limited; an iron block 16 is fixedly arranged on the side wall of the culture dish 3 attached to the two right-angle seats 15 through bolts, the iron block 16 and the magnet 14 are arranged in an mutually attracting mode, and when the culture dish 3 is pushed out, the culture dish 3 can be returned to the original position through the attraction between the magnet 14 and the iron block 16; an observation table 17 is fixedly inserted into the side wall of the box body 1 on the right side of the operation table 2, the left side of the observation table 17 is flush with the operation table 2, the right side of the observation table 17 extends to the right side of the box body 1, and a glass cover 18 is fixedly covered on the extending end; the bottom of the glass cover 18 is fixed on the observation platform 17 through bolts, the left side of the glass cover 18 is fixed on the side wall of the box body 1, the culture dish 3 can be pushed onto the observation platform 17, and an experimenter can directly observe the growth condition of biological cells in the culture dish 3 through the glass cover 18; a water storage tank 19 is fixedly arranged on the left side of the tank body 1, and a water inlet and outlet 20 is fixedly arranged at the bottom of the left side wall of the water storage tank 19 through a bolt and a flange; a water suction pump 21 is fixedly arranged in the water storage tank 19 through bolts, the water suction pump 21 adopts the model of IYG32-300, the output end of the water suction pump 21 is fixedly connected with a main pipe 22, the upper end of the main pipe 22 penetrates through the upper part of the water storage tank 19 and is respectively connected with a spray pipe 23 and a circulating pipe 24 through three-way joints, and the spray pipe 23 and the circulating pipe 24 are both fixedly provided with control valves 25 through bolts and flanges; the right end of the spray pipe 23 penetrates through the left side wall of the box body 1 and is communicated with the connecting pipe 26; the connecting pipe 26 is fixedly arranged on the left side wall inside the box body 1 through a pipe clamp and a bolt; a plurality of atomizing nozzles 27 are fixedly arranged on the connecting pipe 26, the atomizing nozzles 27 extend to the upper part of the operating platform 2, and water in the water storage tank 19 can be pumped into the spraying pipe 23 through the suction pump 32 and then is sprayed out through the atomizing nozzles 27; the right end of the circulating pipe 24 penetrates through the left side wall of the box body 1 and is fixedly arranged on the rear side wall in the box body 1 in a U shape through bolts; a plurality of first heating pipes 28 are fixedly arranged in the water storage tank 19 through bolts, and the heating structure and principle of the first heating pipes 28 are the same as those of the quick heater in the prior art; the electric cabinet 29 is fixedly arranged on the upper portion of the left side wall of the box body 1 through bolts, the electric cabinet 29 is provided with an operation panel, a controller and a power supply, the water suction pump 21, the first heating pipe 28, the driving motor 7 and the push rod 13 are arranged on the operation panel of the electric cabinet 29, the power lines of the driving motor 7 and the push rod 13 penetrate through the side wall of the box body 1 and then are connected with the power supply in the electric cabinet 29, the power lines of the water suction pump 21 and the first heating pipe 28 penetrate through the side wall of the water storage tank 19 and then are connected with the power supply in the electric cabinet 29, and a sealing ring is fixedly arranged at a position penetrating through the side wall of the water storage tank 19.
Further, a drying box 30 is fixedly welded on the top of the box body 1; the top of the box body 1 is fixedly provided with three ventilation holes 31, so that the box body 1 is communicated with the drying box 30; the top of the drying box 30 is fixedly provided with a suction pump 32 through bolts, the suction pump 32 adopts a model TWC-180810, and the input end of the suction pump 32 is communicated with the inside of the drying box 30; a second heating pipe 33 is fixedly arranged inside the drying box 30, the structure and the principle of the second heating pipe 22 are the same as those of a heating wire in the drying box in the prior art, switches of the suction pump 32 and the second heating pipe 33 are arranged on an operation panel of the electric cabinet 29, and power wires of the suction pump 32 and the second heating pipe 33 penetrate through the side wall of the drying box 30 and then are connected with a power supply in the electric cabinet 29; a net cage 34 is fixedly welded at the bottom of the drying box 30, a net plate 35 is fixedly arranged inside the net cage 34, activated carbon 36 is fixedly filled inside the net cage 34 on the upper portion of the net plate 35, calcium oxide particles 37 are fixedly filled inside the net cage 34 at the bottom of the net plate 35, and air can be conveniently filtered and dried; the suction pump 32 sucks the air in the box body 1 into the drying box 30 for drying, the second heating pipe 33 dries the air through a heating method, the activated carbon 36 filters impurities in the air, and the calcium oxide particles 37 adsorb moisture in the air, so that the moist air of the box body 1 is conveniently dried.
Furthermore, a supporting table 38 is fixedly welded on the inner wall of the left side of the box body 1, and a guide wheel 39 is rotatably arranged on the supporting table 38 through a wheel seat; a circular guide rail 40 is fixedly arranged at the bottom of the operating platform 2, and the guide wheel 39 is arranged on the circular guide rail 40 in a rolling manner; the support base 38 supports the left side of the console 2, and the guide wheels 39 roll on the circular guide rails 40, thereby preventing the console 2 from overturning.
Further, a temperature sensor probe 41 is fixedly arranged on the operating platform 2, the temperature sensor probe 41 is an electronic thermometer in the prior art, the temperature sensor probe 41 is connected with a power supply in the electric cabinet 29, the temperature sensor probe 41 detects the temperature condition in the box body 1, and then data is transmitted to a controller in the electric cabinet 29, so that the temperature in the box body 1 can be monitored in real time.
Furthermore, a high liquid level sensor 42 is fixedly arranged on the left side wall of the inner top of the water storage tank 19, a low liquid level sensor 43 is fixedly arranged on the left side wall of the inner bottom of the water storage tank 19, the high liquid level sensor 42 and the low liquid level sensor 43 are both connected with a power supply in the electric cabinet 29, and the high liquid level sensor 42 and the low liquid level sensor 43 are both corrosion-resistant input type liquid level transmitters; the high liquid level sensor 42 and the low liquid level sensor 43 are used for detecting the liquid level condition in the water storage tank 19 and then transmitting data to the controller in the electric cabinet 29, so that the condition that the water level condition in the water storage tank 19 is too high or too low can be effectively prevented.
Further, a three-color warning lamp 44 is fixedly arranged at the top of the water storage tank 19 through a bolt, and the three-color warning lamp 44 is connected with a power supply in the electric cabinet 29; the tricolor warning light is used for prompting the liquid level condition in the water storage tank, and can prompt experimenters when the water level condition in the water storage tank 19 is too high or too low.
Furthermore, an angle adjusting device 45 is fixedly arranged on the right side wall inside the box body 1, a lamp holder 46 is fixedly welded on the left end of the angle adjusting device 45, a lamp holder 47 is fixedly clamped on the lamp holder 46, and the lamp holder 47 is connected with a power supply in the electric cabinet 29, so that the inside of the box body 1 can be conveniently illuminated; the angle adjusting device 45 comprises a first connecting rod 45-1, a second connecting rod 45-2, a first disc 45-3, a second disc 45-4 and a fastening hand screw 45-5; the left end of the first connecting rod 45-1 is fixedly welded with the lamp holder 46, and the right end of the first connecting rod 45-1 is fixedly welded with the first disc 45-3; the right end of the second connecting rod 45-2 is fixedly welded on the right side wall of the box body 1, the left end of the second connecting rod 45-2 is fixedly welded with a second disc 45-4, and the first disc 45-3 is arranged opposite to the second disc 45-4; racks 45-6 are fixedly arranged on the opposite surfaces of the first round disc 45-3 and the second round disc 45-4, and the two racks 45-6 are clamped to prevent the first round disc 45-3 and the second round disc 45-4 from sliding when being matched and connected; the middle parts of the first round disc 45-3 and the second round disc 45-4 are fixedly connected through a fastening hand screw 45-5; the angle between the first connecting rod 45-1 and the second connecting rod 45-2 is adjusted, so that the irradiation angle of the lamp holder 47 can be adjusted, the fastening hand 45-5 is loosened, and the meshing position of the racks 45-6 on the opposite surfaces of the first disc 45-3 and the second disc 45-4 is adjusted, so that the angle between the first connecting rod 45-1 and the second connecting rod 45-2 is adjusted.
The working principle of the specific embodiment is as follows: when the device is used for biological cell culture, the box door of the box body 1 is opened, culture dishes 3 containing culture medium and biological cells are respectively placed between every two right-angle seats 15, the driving motor 7 drives the driving gear 8 to rotate, and the driving gear 8 and the driven gear 9 are connected through the chain 10, so that the driven gear 9 starts to rotate, and the operating platform 2 on the support column 5 is driven to rotate around the shaft column 11; when the culture dish 3 needs to be observed, the driving motor 7 is started, so that the culture dish 3 needing to be observed stops at the right end of the push rod 13, then the driving motor 7 is suspended, the push rod 13 is started, the push rod 13 extends, the magnet 14 and the iron block 16 are attracted to each other, then the culture dish 3 can be pushed onto the observation platform 17, and an experimenter can directly observe the growth condition of biological cells in the culture dish 3 through the glass cover 18; when the interior of the box body 1 needs to be humidified, the control valve 25 on the circulating pipe 24 is closed, the control valve 25 on the spraying pipe 23 is opened, so that water in the water storage tank 19 can be pumped into the spraying pipe 23 through the suction pump 32 and then is sprayed out through the atomizing nozzle 27; when the temperature needs to be heated, the control valve 25 on the circulating pipe 24 is opened, the control valve 25 on the spraying pipe 23 is closed, then the first heating pipe 28 in the water storage tank 19 is heated, so that the temperature of the water in the water storage tank 19 is increased, and then the high-temperature water circulates in the circulating pipe 24 to drive the temperature in the box body 1 to be slowly increased.
After adopting above-mentioned structure, this embodiment beneficial effect does:
1. the water in the water storage tank 19 is conveyed into the spraying pipe 23 from the header pipe 22 by the water suction pump 21, and then is sprayed out by the atomizing nozzle 27 to adjust the humidity in the box body 1, so that the cell culture efficiency can be improved, and the cell survival rate is improved.
2. The push rod 13 extends to attract the magnet 14 and the iron block 16, so that the culture dish 3 is pushed to the observation platform 17 from the operation platform 2, and an experimenter can observe the culture condition of biological cells through the glass cover 18, thereby being convenient for the experimenter to observe and research.
3. A heating pipe 28 in the water storage tank 19 heats the water source in the water storage tank 19, and after heating is completed, hot water is conveyed to the inside of the circulating pipe 24 through the water suction pump 21, so that the inside of the box body 1 can be heated, and the survival efficiency of cells can be improved.
The above description is only for the purpose of illustrating the technical solutions of the present invention and not for the purpose of limiting the same, and other modifications or equivalent substitutions made by those skilled in the art to the technical solutions of the present invention should be covered within the scope of the claims of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (1)
1. A biological cell culture device comprises a box body (1), an operation platform (2) and a culture dish (3); an operation table (2) is arranged in the box body (1), and a plurality of culture dishes (3) are arranged on the operation table (2) in a round angle manner; the method is characterized in that: the device also comprises a driving motor (7), a water storage tank (19), an electric cabinet (29), an observation platform (17) and a glass cover (18); a base (4) is vertically and fixedly arranged at the inner bottom of the box body (1), a support column (5) is vertically and fixedly arranged at the bottom of the operating platform (2), and the upper part of the base (4) is fixedly connected with the bottom of the support column (5) through a flat-top thrust ball bearing (6); a driving motor (7) is fixedly arranged in the box body (1) on the right side of the base (4), and a driving gear (8) is fixedly arranged on the output end of the driving motor (7); a driven gear (9) is fixedly sleeved on the outer side wall of the supporting column (5), and the driven gear (9) is connected with the driving gear (8) through a chain (10); an axial column (11) is vertically and fixedly arranged at the inner bottom of the box body (1), and the axial column (11) is rotatably arranged in the middle of the operating platform (2), the supporting column (5) and the base (4) in a penetrating manner through a bearing; a fixed seat (12) is fixedly arranged at the upper end of the shaft column (11), and a push rod (13) is fixedly arranged on the fixed seat (12); a magnet (14) is fixedly arranged on the end head of the push rod (13); a plurality of right-angle seats (15) are fixedly arranged on the operating platform (2), every two right-angle seats (15) are arranged oppositely, and the culture dish (3) is arranged between the two opposite right-angle seats (15); an iron block (16) is fixedly arranged on the side wall of the culture dish (3) which is attached to the two right-angle seats (15), and the iron block (16) and the magnet (14) are mutually attracted; an observation table (17) is fixedly inserted into the side wall of the box body (1) on the right side of the operation table (2), the left side of the observation table (17) is flush with the operation table (2), the right side of the observation table (17) extends to the right side of the box body (1), and a glass cover (18) is fixedly covered on the extending end; the bottom of the glass cover (18) is fixed on the observation platform (17), and the left side of the glass cover (18) is fixed on the side wall of the box body (1); a water storage tank (19) is fixedly arranged on the left side of the tank body (1), and a water inlet and outlet (20) is fixedly arranged at the bottom of the left side wall of the water storage tank (19); a water suction pump (21) is fixedly arranged in the water storage tank (19), the output end of the water suction pump (21) is fixedly connected with a header pipe (22), the upper end of the header pipe (22) penetrates through the upper part of the water storage tank (19) and then is respectively connected with a spray pipe (23) and a circulating pipe (24) through a three-way joint, and control valves (25) are fixedly arranged on the spray pipe (23) and the circulating pipe (24); the right end of the spray pipe (23) penetrates through the left side wall of the box body (1) and is communicated with the connecting pipe (26); the connecting pipe (26) is fixedly arranged on the left side wall inside the box body (1) through a pipe clamp; a plurality of atomizing nozzles (27) are fixedly arranged on the connecting pipe (26), and the atomizing nozzles (27) extend to the upper part of the operating platform (2); the right end of the circulating pipe (24) penetrates through the left side wall of the box body (1) and is fixedly arranged on the rear side wall in the box body (1) in a U shape; a plurality of first heating pipes (28) are fixedly arranged inside the water storage tank (19); an electric cabinet (29) is fixedly arranged on the upper part of the left side wall of the box body (1), and the driving motor (7), the push rod (13) and the first heating pipe (28) are all connected with a power supply in the electric cabinet (29);
a drying box (30) is fixedly arranged at the top of the box body (1); the top of the box body (1) is fixedly provided with a plurality of vent holes (31); a suction pump (32) is fixedly arranged at the top of the drying box (30), and the input end of the suction pump (32) is communicated with the inside of the drying box (30); a second heating pipe (33) is fixedly arranged inside the drying box (30), and the suction pump (32) and the second heating pipe (33) are both connected with a power supply in the electric cabinet (29); a net cage (34) is fixedly arranged at the bottom of the drying box (30), a net plate (35) is fixedly arranged in the net cage (34), activated carbon (36) is fixedly filled in the net cage (34) at the upper part of the net plate (35), and calcium oxide particles (37) are fixedly filled in the net cage (34) at the bottom of the net plate (35); the air in the box body (1) is sucked into the drying box (30) by the suction pump (32) to be dried, the air is dried by the heating pipe (33) in a heating way, impurities in the air are filtered by the active carbon (36), and moisture in the air is adsorbed by the calcium oxide particles (37); a supporting table (38) is fixedly arranged on the inner wall of the left side of the box body (1), and a guide wheel (39) is rotatably arranged on the supporting table (38) through a wheel seat; a circular guide rail (40) is fixedly arranged at the bottom of the operating platform (2), and the guide wheel (39) is arranged on the circular guide rail (40) in a rolling manner; the supporting table (38) supports the left side of the operating table (2), and the guide wheel (39) rolls on the circular guide rail (40); a temperature sensor probe (41) is fixedly arranged on the operating platform (2), the temperature sensor probe (41) is connected with a power supply in the electric cabinet (29), the temperature sensor probe (41) detects the temperature condition in the box body (1), and then data are transmitted to a controller in the electric cabinet (29); a high liquid level sensor (42) is fixedly arranged on the left side wall of the inner top of the water storage tank (19), a low liquid level sensor (43) is fixedly arranged on the left side wall of the inner bottom of the water storage tank (19), and the high liquid level sensor (42) and the low liquid level sensor (43) are both connected with a power supply in the electric cabinet (29); the high liquid level sensor (42) and the low liquid level sensor (43) are used for detecting the liquid level condition in the water storage tank (19) and then transmitting data to a controller in the electric cabinet (29); a three-color warning lamp (44) is fixedly arranged at the top of the water storage tank (19), and the three-color warning lamp (44) is connected with a power supply in the electric cabinet (29); the three-color warning lamp (44) is used for prompting the liquid level condition in the water storage tank (19); an angle adjusting device (45) is fixedly arranged on the right side wall inside the box body (1), a lamp holder (46) is fixedly arranged at the left end of the angle adjusting device (45), a lamp holder (47) is fixedly clamped on the lamp holder (46), and the lamp holder (47) is connected with a power supply in the electric cabinet (29); the angle adjusting device (45) comprises a first connecting rod (45-1), a second connecting rod (45-2), a first disc (45-3), a second disc (45-4) and a fastening hand screw (45-5); the left end of the first connecting rod (45-1) is fixedly connected with the lamp holder (46), and the right end of the first connecting rod (45-1) is fixedly connected with the first disc (45-3); the right end of the second connecting rod (45-2) is fixedly arranged on the right side wall of the box body (1), the left end of the second connecting rod (45-2) is fixedly connected with a second disc (45-4), and the first disc (45-3) and the second disc (45-4) are arranged oppositely; racks (45-6) are fixedly arranged on the opposite surfaces of the first disc (45-3) and the second disc (45-4), and the two racks (45-6) are clamped; the middle parts of the first disc (45-3) and the second disc (45-4) are fixedly connected through a fastening hand screw (45-5); adjusting the angle between the first connecting rod (45-1) and the second connecting rod (45-2), so that the irradiation angle of the lamp holder (47) can be adjusted, loosening the fastening hand (45-5), and adjusting the meshing position of the racks (45-6) on the opposite surfaces of the first disc (45-3) and the second disc (45-4) to adjust the angle between the first connecting rod (45-1) and the second connecting rod (45-2);
opening a box door of the box body (1), respectively placing culture dishes (3) containing culture media and biological cells between every two right-angle seats (15), driving a driving gear (8) to rotate by a driving motor (7), and enabling the driven gear (9) to start to rotate as the driving gear (8) is connected with the driven gear (9) through a chain (10), so that an operation table (2) on a support column (5) is driven to start to rotate around a shaft column (11); when the culture dish (3) needs to be observed, the driving motor (7) is started, the culture dish (3) needing to be observed is stopped at the right end of the push rod (13), then the driving motor (7) is suspended, the push rod (13) is started, the push rod (13) is extended, the magnet (14) and the iron block (16) are attracted to each other, then the culture dish (3) can be pushed onto the observation platform (17), and the growth condition of biological cells in the culture dish (3) is observed directly through the glass cover (18); when the box body (1) needs to be humidified, the control valve (25) on the circulating pipe (24) is closed, the control valve (25) on the spraying pipe (23) is opened, so that water in the water storage tank (19) can be pumped into the spraying pipe (23) through the suction pump (32), and then is sprayed out through the atomizing nozzle (27); when the temperature needs to be heated, the control valve (25) on the circulating pipe (24) is opened, the control valve (25) on the spraying pipe (23) is closed, then the first heating pipe (28) in the water storage tank (19) is heated, so that the temperature of the water in the water storage tank (19) is increased, and then the high-temperature water circulates in the circulating pipe (24) to drive the temperature in the box body (1) to be slowly increased.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811558913.XA CN109593653B (en) | 2018-12-13 | 2018-12-13 | Biological cell culture device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811558913.XA CN109593653B (en) | 2018-12-13 | 2018-12-13 | Biological cell culture device |
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| CN109593653A CN109593653A (en) | 2019-04-09 |
| CN109593653B true CN109593653B (en) | 2021-10-01 |
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| CN111621410A (en) * | 2020-06-29 | 2020-09-04 | 江苏康正生物科技有限公司 | A incubator for food detection |
| CN112625906B (en) * | 2020-12-30 | 2022-11-18 | 深圳乐土沃森精准医疗有限公司 | Special culture dish of cardiac muscle cell and nerve cell |
| CN112481129B (en) * | 2021-01-15 | 2021-07-09 | 北京达济康华生物科技有限责任公司 | Cell culture instrument for immune cell amplification |
| CN113462568B (en) * | 2021-08-12 | 2023-07-28 | 杭州中赢生物医疗科技有限公司 | Human peripheral blood NK cell culture device |
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|---|---|---|---|---|
| CN104862210A (en) * | 2015-04-29 | 2015-08-26 | 李郝 | Microbiological detection cultivating device |
| CN107338190A (en) * | 2017-07-26 | 2017-11-10 | 青岛市海慈医疗集团 | A kind of clinical laboratory's anti-pollution type germ culturing device |
| CN208104439U (en) * | 2018-03-16 | 2018-11-16 | 长沙医学院 | A kind of atomizing type cell culture apparatus |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR101660852B1 (en) * | 2008-12-23 | 2016-09-28 | 조마 (유에스) 엘엘씨 | Flexible manufacturing system |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104862210A (en) * | 2015-04-29 | 2015-08-26 | 李郝 | Microbiological detection cultivating device |
| CN107338190A (en) * | 2017-07-26 | 2017-11-10 | 青岛市海慈医疗集团 | A kind of clinical laboratory's anti-pollution type germ culturing device |
| CN208104439U (en) * | 2018-03-16 | 2018-11-16 | 长沙医学院 | A kind of atomizing type cell culture apparatus |
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