CN107446814B - Equipment is deposited with culture medium of adjustable temperature to biology - Google Patents

Equipment is deposited with culture medium of adjustable temperature to biology Download PDF

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Publication number
CN107446814B
CN107446814B CN201710742097.7A CN201710742097A CN107446814B CN 107446814 B CN107446814 B CN 107446814B CN 201710742097 A CN201710742097 A CN 201710742097A CN 107446814 B CN107446814 B CN 107446814B
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box
air
culture medium
storage box
pipe
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CN107446814A (en
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黄轩
黎尼平
蒋雅婷
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Wan'an Maosheng Information Technology Co ltd
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Yiwu Tiancheng Technology Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers

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  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Biomedical Technology (AREA)
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  • Thermal Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to culture medium storage equipment, in particular to biological temperature-adjustable culture medium storage equipment. The invention aims to provide a temperature-adjustable culture medium storage device for organisms, which can adjust the temperature of a culture medium, provide a sterile environment for the culture medium and store a plurality of culture media. In order to solve the technical problem, the invention provides a temperature-adjustable culture medium storage device for organisms, which comprises a storage box, a glass door, a heating mechanism, a refrigerating mechanism, an air inlet box, a first screen plate, a filter layer, a sterilization layer, a third air pump, an air inlet pipe, a one-way valve and the like; the front part of the storage box is provided with a glass door, and the top part of the storage box is provided with a heating mechanism and a refrigerating mechanism. The invention can adjust the temperature of the culture medium, provide a sterile environment for the culture medium and store a plurality of culture media, and the drying agent can dry the storage box to prevent bacteria from breeding due to over humidity in the storage box.

Description

Equipment is deposited with culture medium of adjustable temperature to biology
Technical Field
The invention relates to culture medium storage equipment, in particular to biological temperature-adjustable culture medium storage equipment.
Background
A living being is a living being with kinetic energy, also a collection of objects, whereas an individual living being refers to an organism, as opposed to a non-biological one.
The culture medium is an artificially prepared nutrient for growth and maintenance of microorganisms, plant tissues and animal tissues, generally contains carbohydrate, nitrogenous substances, inorganic salts including trace elements, vitamins, water and the like, and also contains antibiotics, pigments, hormones and serum, and is slightly different in storage and preservation aspects due to different prepared raw materials and different use requirements, and the liquid culture medium is not easy to store for a long time and is changed into powder.
In biological research, culture media are often manufactured for observation, and the culture media are stored in an environment which needs proper temperature and sterility, so that the conventional culture media storage equipment has the defects that the temperature of the culture media cannot be adjusted, the sterile environment cannot be provided for the culture media, and a plurality of culture media cannot be stored.
Disclosure of Invention
(1) Technical problem to be solved
The invention aims to overcome the defects that the temperature of a culture medium cannot be adjusted, a sterile environment cannot be provided for the culture medium, and a plurality of culture media cannot be stored in the conventional culture medium storage equipment.
(2) Technical scheme
In order to solve the technical problems, the invention provides temperature-adjustable culture medium storage equipment for biology, which comprises a storage box, a glass door, a heating mechanism, a refrigerating mechanism, an air inlet box, a first screen plate, a filter layer, a sterilization layer, a third air pump, an air inlet pipe, a one-way valve, a first support rod, a placing frame and a second screen plate, wherein the glass door is arranged at the front part of the storage box, the heating mechanism and the refrigerating mechanism are arranged at the top part of the storage box, the heating mechanism is positioned at the left side of the refrigerating mechanism, the air inlet box is arranged at the top parts of the heating mechanism and the refrigerating mechanism, the first screen plate is arranged at the top part of the air inlet box, the filter layer and the sterilization layer are arranged between the left wall and the right wall in the air inlet box, the third air pump is arranged at the middle of the bottom part in the air inlet box, the air inlet pipes are symmetrically arranged at the left side, the first supporting rod is evenly provided with a placing rack, and the right side of the bottom of the storage box is provided with a second screen plate.
Preferably, heating mechanism is including the heating cabinet, a housing, the heating wire, first air pump, first gas-supply pipe, first outlet duct and first shower nozzle, it is equipped with the heating cabinet to deposit roof portion left side, the left intake pipe passes the heating cabinet roof portion, top and interior bottom all are equipped with the casing in the heating cabinet, be equipped with the heating wire in the casing, bottom left side is equipped with first air pump in the heating cabinet, be equipped with first gas-supply pipe on the first air pump, first gas-supply pipe lower part is equipped with first outlet duct, first outlet duct passes deposits roof portion, first outlet duct right side evenly is equipped with first shower nozzle.
Preferably, the refrigeration mechanism is including the refrigeration case, the refrigerator, the second air pump, the second gas-supply pipe, second outlet duct and second shower nozzle, it is equipped with the refrigeration case to deposit roof portion right side, right-hand intake pipe passes the refrigeration roof portion, refrigeration incasement top is equipped with the refrigerator, refrigeration incasement bottom right side is equipped with the second air pump, be equipped with the second gas-supply pipe on the second air pump, second gas-supply pipe lower part is equipped with the second outlet duct, the second outlet duct passes the refrigeration roof portion, first outlet duct left side evenly is equipped with the second shower nozzle.
Preferably, the storage box further comprises a second supporting rod, a first sliding rail, a bearing seat, an electric reel, a pull rope, a first sliding block, a rack, a gear and a third supporting rod, the second supporting rod and the first sliding rail are arranged at the bottom in the storage box, the first sliding rail is located between the left and right second supporting rods, the bearing seat is symmetrically arranged at the top and the inner bottom in the storage box, the bearing seat is located in front of the first sliding rail, the third supporting rod is installed between the upper and lower bearing seats, the placing frame is arranged on the third supporting rod, the gear is arranged on the lower portion of the third supporting rod, the electric reel is arranged at the upper end of the left and right second supporting rods, the pull rope is wound on the electric reel, the first sliding block is symmetrically arranged on the left and right sides of the top of the first sliding rail, the left and right sides of the first sliding block are connected with the.
Preferably, the multifunctional drawer further comprises a first supporting plate, a second sliding rail, a second sliding block, a drawer and a handle, the first supporting plate is evenly arranged on the third supporting rod, the second sliding rail is symmetrically arranged on the left side and the right side of the top of the first supporting plate, the second sliding block is arranged on the second sliding rail, the drawer is arranged between the left second sliding block and the right second sliding block, a groove is formed in the middle of the drawer, the third supporting rod penetrates through the groove, and the handle is arranged on the front portion of the drawer.
Preferably, the drying device further comprises a second supporting plate, a placing frame and a drying agent, the second supporting plate is arranged on the left side of the lower portion of the third supporting rod and located above the gear, the placing frame is arranged on the top of the second supporting plate, and the drying agent is arranged in the placing frame.
Preferably, the glass door further comprises a thermometer, a temperature sensor and a controller, the thermometer is arranged on the left side of the rear portion of the glass door, the temperature sensor and the controller are arranged on the right side of the rear portion of the glass door, the temperature sensor is located above the controller, and the temperature sensor is connected with the controller.
The working principle is as follows: in biological research, culture media are often manufactured for observation, and because the culture media need proper temperature and sterile environment for storage, therefore, people can store the culture medium in the device, firstly people put the culture medium on the placing rack through the glass door, then the heating mechanism is started to heat the storage box according to the temperature condition required by the culture medium, or people start the refrigeration mechanism to refrigerate the storage box, the temperature of the storage box is adjusted to meet the temperature condition of the culture medium, outside air enters the air inlet box through the first screen plate, and after the air is filtered and sterilized through the filter layer and the sterilization layer, the environment in the culture medium can not be destroyed, then in gaseous passing through third air pump and intake pipe entering heating mechanism or refrigeration mechanism, the second otter board can be discharged and guarantee to deposit the atmospheric pressure balance of incasement, and when the culture medium was observed to needs, people took out through the glass door the culture medium can.
Because the heating mechanism comprises a heating box, a shell, an electric heating wire, a first air pump, a first air pipe, a first air outlet pipe and a first spray head, the heating box is arranged on the left side of the top of the storage box, the left air inlet pipe penetrates through the top of the heating box, the shell is arranged on the top and the inner bottom of the heating box, the electric heating wire is arranged in the shell, the first air pump is arranged on the left side of the inner bottom of the heating box, the first air pipe is arranged on the first air pump, the first air outlet pipe is arranged on the lower part of the first air pipe, the first air outlet pipe penetrates through the top of the storage box, when the temperature of the storage box needs to be raised, people start the electric heating wire to heat the gas entering the heating box, then start the first air pump to convey the gas into the first gas conveying pipe and the first gas outlet pipe, and spray the hot gas through the first spray head, thereby the temperature in the storage box is raised, and the electric heating wire and the first air pump are closed after the temperature reaches the temperature required by the culture medium.
Because the refrigerating mechanism comprises a refrigerating box, a refrigerator, a second air pump, a second air pipe, a second air outlet pipe and a second spray head, the refrigerating box is arranged on the right side of the top of the storage box, the right air inlet pipe penetrates through the top of the refrigerating box, the refrigerator is arranged on the top of the refrigerating box, the second air pump is arranged on the right side of the bottom of the refrigerating box, the second air pipe is arranged on the second air pump, the second air outlet pipe is arranged on the lower portion of the second air pipe, the second air outlet pipe penetrates through the top of the refrigerating box, the second spray head is uniformly arranged on the left, therefore, when the storage box needs to be refrigerated, people start the refrigerator to refrigerate the air entering the refrigeration box, then start the second air pump to convey air into the second air conveying pipe and the second air outlet pipe, spray the cold air out through the second spray head, thereby reducing the temperature in the storage box and turning off the refrigerator and the second air pump after the temperature reaches the temperature required by the culture medium.
Because the culture box also comprises a second supporting rod, a first sliding rail, a bearing seat, an electric reel, a pull rope, a first sliding block, a rack, a gear and a third supporting rod, the second supporting rod and the first sliding rail are arranged at the bottom in the storage box, the first sliding rail is positioned between the left and right second supporting rods, the bearing seats are symmetrically arranged at the top and the inner bottom in the storage box, the bearing seats are positioned in front of the first sliding rail, the third supporting rod is arranged between the upper and lower bearing seats, the placing frame is arranged on the third supporting rod, the gear is arranged at the lower part of the third supporting rod, the electric reels are arranged at the upper ends of the left and right second supporting rods, the pull rope is wound on the electric reels, the first sliding blocks are symmetrically arranged at the left and right sides of the top of the first sliding rail, the left and right sides of the first sliding blocks are connected with the pull rope, the racks are arranged at, people can start the stay cord is put to the electronic reel in left, the stay cord is received to right-hand electronic reel, thereby drive the rack and remove right, and then make the gear drive the culture medium on the rack rotatory, go to near first shower nozzle department when the culture medium, people close electronic reel can, can heat the culture medium to required temperature fast like this, when needs heat the culture medium, people can start the electronic reel in left and receive the stay cord, the stay cord is put to right-hand electronic reel, thereby drive the rack and remove left, and then make the gear drive the culture medium rotation on the rack, go to near second shower nozzle department when the culture medium, people close electronic reel can, thereby refrigerate the culture medium to required temperature fast, can prevent like this heating or refrigeration time overlength and make the culture medium internal environment have the change to some extent.
Because still including first backup pad, the second slide rail, the second slider, drawer and handle, evenly be equipped with first backup pad on the third branch, first backup pad top left and right sides symmetry is equipped with the second slide rail, be equipped with the second slider on the second slide rail, be equipped with the drawer between the second slider of left and right sides, open flutedly in the middle of the drawer, third branch passes the recess, the drawer front portion is equipped with the handle, so people can pull out the drawer through the handle, then put the culture medium on the drawer, later people push back the drawer and reset can, second slide rail and second slider make people's pull drawer that can be easier, when people will take out the culture medium, repeat above-mentioned operation can, make things convenient for people to get through the pull drawer and put the culture medium.
Because still including the second backup pad, place frame and drier, third branch lower part left side is equipped with the second backup pad, and the second backup pad is located the gear top, and second backup pad top is equipped with places the frame, places and is equipped with the drier in the frame, so the drier can be to depositing the case and drying, prevents to deposit the incasement too moist and breed the bacterium to influence depositing of culture medium.
Because still including the thermometer, temperature sensor and controller, glass door rear portion left side is equipped with the thermometer, glass door rear portion right side is equipped with temperature sensor and controller, temperature sensor is located the controller top, temperature sensor is connected with the controller, so people can observe the thermometer through the glass door, thereby know the temperature value of depositing the incasement, because the culture medium needs to be deposited under the suitable temperature, when temperature sensor detects to deposit incasement temperature and is less than its default, temperature sensor feeds back the signal that detects to the controller, the work of controller control heating mechanism, thereby make and deposit the case intensification, when temperature sensor detects to deposit incasement temperature and is higher than its default, temperature sensor feeds back the signal that detects to the controller, the work of controller control refrigeration mechanism, thereby make and deposit the case refrigeration.
The controller selects a PLC controller with a model S24S0T, which is produced by Xiamenhai science and technology limited, and has 24VDC power supply, 16 input channels and 8 output channels outside the host. The definition of input-output port is: x0 is on, X1 is off, and X2 is a temperature sensor; y0 is a heating wire, Y1 is a first air pump, Y2 is a refrigerator, Y3 is a second air pump, Y4 is a third air pump, and Y5 is an electric reel. According to the electrical control schematic diagram shown in fig. 10, those skilled in the art can control each component to operate according to the above-mentioned working principle by programming without creative labor, and related instructions of programming are all prior art and will not be described herein again.
The control mode of the invention is automatically controlled by the controller, the control circuit of the controller can be realized by simple programming of a person skilled in the art, the invention belongs to the common knowledge in the field, and the invention is mainly used for protecting mechanical devices, so the control mode and the circuit connection are not explained in detail in the invention.
(3) Advantageous effects
The invention can adjust the temperature of the culture medium, provide a sterile environment for the culture medium and store a plurality of culture media, the environment in the culture medium cannot be damaged after the gas is filtered and sterilized by the filter layer and the sterilization layer, and the drying agent can dry the storage box to prevent the storage box from being too humid and breeding bacteria, thereby influencing the storage of the culture medium.
Drawings
Fig. 1 is a first front view structure diagram of the present invention.
Fig. 2 is a second front view structure diagram of the present invention.
Fig. 3 is a schematic front view of the heating mechanism of the present invention.
Fig. 4 is a schematic front view of the refrigeration mechanism of the present invention.
Fig. 5 is a first partial schematic front view structure of the present invention.
Fig. 6 is a second partial schematic front view structure of the present invention.
Fig. 7 is a partial top view of the present invention.
Fig. 8 is a third partial schematic front view structure of the present invention.
Fig. 9 is a front view of a fourth partial structure of the present invention.
Fig. 10 is an electrical control schematic of the present invention.
The labels in the figures are: 1-storage box, 2-glass door, 3-heating mechanism, 301-heating box, 302-shell, 303-heating wire, 304-first air pump, 305-first air pipe, 306-first air outlet pipe, 307-first spray head, 4-refrigerating mechanism, 41-refrigerating box, 42-refrigerator, 43-second air pump, 44-second air pipe, 45-second air outlet pipe, 46-second spray head, 5-air inlet box, 6-first mesh plate, 7-filter layer, 8-sterilization layer, 9-third air pump, 10-air inlet pipe, 11-one-way valve, 12-first support rod, 13-placing rack, 14-second support rod, 15-first sliding rail, 16-bearing seat, 17-electric reel, 18-a pull rope, 19-a first sliding block, 20-a rack, 21-a gear, 22-a first supporting plate, 23-a second sliding rail, 24-a second sliding block, 25-a drawer, 26-a groove, 27-a handle, 28-a second supporting plate, 29-a placing frame, 30-a drying agent, 31-a thermometer, 32-a temperature sensor, 33-a controller, 34-a third supporting rod and 35-a second screen plate.
Detailed Description
The invention is further described below with reference to the figures and examples.
Example 1
A temperature-adjustable culture medium storage device for biology is disclosed, as shown in figures 1-10, and comprises a storage box 1, a glass door 2, a heating mechanism 3, a refrigerating mechanism 4, an air inlet box 5, a first screen plate 6, a filter layer 7, a sterilization layer 8, a third air pump 9, an air inlet pipe 10, a one-way valve 11, a first support rod 12, a placing frame 13 and a second screen plate 35, wherein the glass door 2 is arranged at the front part of the storage box 1, the heating mechanism 3 and the refrigerating mechanism 4 are arranged at the top part of the storage box 1, the heating mechanism 3 is positioned at the left side of the refrigerating mechanism 4, the air inlet box 5 is arranged at the top parts of the heating mechanism 3 and the refrigerating mechanism 4, the first screen plate 6 is arranged at the top part of the air inlet box 5, the filter layer 7 and the sterilization layer 8 are arranged between the left wall and the right wall in the air inlet box 5, the filter layer 7 is positioned above the sterilization layer, the lower parts of the left and right air inlet pipes 10 are respectively provided with a one-way valve 11, a first supporting rod 12 is arranged between the upper wall and the lower wall in the storage box 1, the first supporting rod 12 is evenly provided with a placing frame 13, and the right side of the bottom of the storage box 1 is provided with a second screen plate 35.
Example 2
A temperature-adjustable culture medium storage device for biology is disclosed, as shown in figures 1-10, and comprises a storage box 1, a glass door 2, a heating mechanism 3, a refrigerating mechanism 4, an air inlet box 5, a first screen plate 6, a filter layer 7, a sterilization layer 8, a third air pump 9, an air inlet pipe 10, a one-way valve 11, a first support rod 12, a placing frame 13 and a second screen plate 35, wherein the glass door 2 is arranged at the front part of the storage box 1, the heating mechanism 3 and the refrigerating mechanism 4 are arranged at the top part of the storage box 1, the heating mechanism 3 is positioned at the left side of the refrigerating mechanism 4, the air inlet box 5 is arranged at the top parts of the heating mechanism 3 and the refrigerating mechanism 4, the first screen plate 6 is arranged at the top part of the air inlet box 5, the filter layer 7 and the sterilization layer 8 are arranged between the left wall and the right wall in the air inlet box 5, the filter layer 7 is positioned above the sterilization layer, the lower parts of the left and right air inlet pipes 10 are respectively provided with a one-way valve 11, a first supporting rod 12 is arranged between the upper wall and the lower wall in the storage box 1, the first supporting rod 12 is evenly provided with a placing frame 13, and the right side of the bottom of the storage box 1 is provided with a second screen plate 35.
Heating mechanism 3 is including heating cabinet 301, casing 302, heating wire 303, first air pump 304, first air pipe 305, first outlet duct 306 and first shower nozzle 307, it is equipped with heating cabinet 301 to deposit 1 top left side, left air pipe 10 passes heating cabinet 301 top, top and interior bottom all are equipped with casing 302 in heating cabinet 301, be equipped with heating wire 303 in the casing 302, bottom left side is equipped with first air pump 304 in heating cabinet 301, be equipped with first air pipe 305 on the first air pump 304, first air pipe 305 lower part is equipped with first outlet duct 306, first outlet duct 306 passes and deposits 1 top of case, first outlet duct 306 right side evenly is equipped with first shower nozzle 307.
Example 3
A temperature-adjustable culture medium storage device for biology is disclosed, as shown in figures 1-10, and comprises a storage box 1, a glass door 2, a heating mechanism 3, a refrigerating mechanism 4, an air inlet box 5, a first screen plate 6, a filter layer 7, a sterilization layer 8, a third air pump 9, an air inlet pipe 10, a one-way valve 11, a first support rod 12, a placing frame 13 and a second screen plate 35, wherein the glass door 2 is arranged at the front part of the storage box 1, the heating mechanism 3 and the refrigerating mechanism 4 are arranged at the top part of the storage box 1, the heating mechanism 3 is positioned at the left side of the refrigerating mechanism 4, the air inlet box 5 is arranged at the top parts of the heating mechanism 3 and the refrigerating mechanism 4, the first screen plate 6 is arranged at the top part of the air inlet box 5, the filter layer 7 and the sterilization layer 8 are arranged between the left wall and the right wall in the air inlet box 5, the filter layer 7 is positioned above the sterilization layer, the lower parts of the left and right air inlet pipes 10 are respectively provided with a one-way valve 11, a first supporting rod 12 is arranged between the upper wall and the lower wall in the storage box 1, the first supporting rod 12 is evenly provided with a placing frame 13, and the right side of the bottom of the storage box 1 is provided with a second screen plate 35.
Heating mechanism 3 is including heating cabinet 301, casing 302, heating wire 303, first air pump 304, first air pipe 305, first outlet duct 306 and first shower nozzle 307, it is equipped with heating cabinet 301 to deposit 1 top left side, left air pipe 10 passes heating cabinet 301 top, top and interior bottom all are equipped with casing 302 in heating cabinet 301, be equipped with heating wire 303 in the casing 302, bottom left side is equipped with first air pump 304 in heating cabinet 301, be equipped with first air pipe 305 on the first air pump 304, first air pipe 305 lower part is equipped with first outlet duct 306, first outlet duct 306 passes and deposits 1 top of case, first outlet duct 306 right side evenly is equipped with first shower nozzle 307.
Refrigerating mechanism 4 is including refrigeration case 41, refrigerator 42, second air pump 43, second air supply pipe 44, second outlet duct 45 and second shower nozzle 46, it is equipped with refrigeration case 41 to deposit 1 top right side, right-hand intake pipe 10 passes refrigeration case 41 top, the top is equipped with refrigerator 42 in the refrigeration case 41, bottom right side is equipped with second air pump 43 in the refrigeration case 41, be equipped with second air supply pipe 44 on the second air pump 43, second air supply pipe 44 lower part is equipped with second outlet duct 45, second outlet duct 45 passes refrigeration case 41 top, first outlet duct 306 left side evenly is equipped with second shower nozzle 46.
Example 4
A temperature-adjustable culture medium storage device for biology is disclosed, as shown in figures 1-10, and comprises a storage box 1, a glass door 2, a heating mechanism 3, a refrigerating mechanism 4, an air inlet box 5, a first screen plate 6, a filter layer 7, a sterilization layer 8, a third air pump 9, an air inlet pipe 10, a one-way valve 11, a first support rod 12, a placing frame 13 and a second screen plate 35, wherein the glass door 2 is arranged at the front part of the storage box 1, the heating mechanism 3 and the refrigerating mechanism 4 are arranged at the top part of the storage box 1, the heating mechanism 3 is positioned at the left side of the refrigerating mechanism 4, the air inlet box 5 is arranged at the top parts of the heating mechanism 3 and the refrigerating mechanism 4, the first screen plate 6 is arranged at the top part of the air inlet box 5, the filter layer 7 and the sterilization layer 8 are arranged between the left wall and the right wall in the air inlet box 5, the filter layer 7 is positioned above the sterilization layer, the lower parts of the left and right air inlet pipes 10 are respectively provided with a one-way valve 11, a first supporting rod 12 is arranged between the upper wall and the lower wall in the storage box 1, the first supporting rod 12 is evenly provided with a placing frame 13, and the right side of the bottom of the storage box 1 is provided with a second screen plate 35.
Heating mechanism 3 is including heating cabinet 301, casing 302, heating wire 303, first air pump 304, first air pipe 305, first outlet duct 306 and first shower nozzle 307, it is equipped with heating cabinet 301 to deposit 1 top left side, left air pipe 10 passes heating cabinet 301 top, top and interior bottom all are equipped with casing 302 in heating cabinet 301, be equipped with heating wire 303 in the casing 302, bottom left side is equipped with first air pump 304 in heating cabinet 301, be equipped with first air pipe 305 on the first air pump 304, first air pipe 305 lower part is equipped with first outlet duct 306, first outlet duct 306 passes and deposits 1 top of case, first outlet duct 306 right side evenly is equipped with first shower nozzle 307.
Refrigerating mechanism 4 is including refrigeration case 41, refrigerator 42, second air pump 43, second air supply pipe 44, second outlet duct 45 and second shower nozzle 46, it is equipped with refrigeration case 41 to deposit 1 top right side, right-hand intake pipe 10 passes refrigeration case 41 top, the top is equipped with refrigerator 42 in the refrigeration case 41, bottom right side is equipped with second air pump 43 in the refrigeration case 41, be equipped with second air supply pipe 44 on the second air pump 43, second air supply pipe 44 lower part is equipped with second outlet duct 45, second outlet duct 45 passes refrigeration case 41 top, first outlet duct 306 left side evenly is equipped with second shower nozzle 46.
The storage box also comprises a second support rod 14, a first slide rail 15, a bearing seat 16, an electric reel 17, a pull rope 18, a first slide block 19, a rack 20, a gear 21 and a third support rod 34, wherein the second support rod 14 and the first slide rail 15 are arranged at the bottom in the storage box 1, the first slide rail 15 is positioned in the middle of the second support rods 14 on the left and right sides, the bearing seats 16 are symmetrically arranged at the top and the inner bottom in the storage box 1, the bearing seats 16 are positioned in front of the first slide rail 15, the third support rod 34 is arranged between the bearing seats 16 on the upper and lower sides, a placing frame 13 is arranged on the third support rod 34, the gear 21 is arranged at the lower part of the third support rod 34, the electric reel 17 is arranged at the upper ends of the second support rods 14 on the left and right sides, the pull rope 18 is wound on the electric reel 17, the first slide blocks 19 are symmetrically arranged on the left and right sides of the top of the first slide, the rack 20 is located behind the gear 21, and the rack 20 is engaged with the gear 21.
Still including first backup pad 22, second slide rail 23, second slider 24, drawer 25 and handle 27, evenly be equipped with first backup pad 22 on the third branch pole 34, first backup pad 22 top left and right sides symmetry is equipped with second slide rail 23, is equipped with second slider 24 on the second slide rail 23, is equipped with drawer 25 between the left and right sides two side second sliders 24, and open in the middle of the drawer 25 has recess 26, and third branch pole 34 passes recess 26, and drawer 25 front portion is equipped with handle 27.
The rack is characterized by further comprising a second supporting plate 28, a placing frame 29 and a drying agent 30, wherein the second supporting plate 28 is arranged on the left side of the lower portion of the third supporting rod 34, the second supporting plate 28 is located above the gear 21, the placing frame 29 is arranged on the top of the second supporting plate 28, and the drying agent 30 is arranged in the placing frame 29.
The glass door is characterized by further comprising a thermometer 31, a temperature sensor 32 and a controller 33, wherein the thermometer 31 is arranged on the left side of the rear portion of the glass door 2, the temperature sensor 32 and the controller 33 are arranged on the right side of the rear portion of the glass door 2, the temperature sensor 32 is located above the controller 33, and the temperature sensor 32 is connected with the controller 33.
The working principle is as follows: in biological research, culture media are often manufactured for observation, because the culture media are stored in an environment which needs proper temperature and is aseptic, people can store the culture media in the device, firstly people put the culture media on the placing rack 13 through the glass door 2, then according to the temperature condition needed by the culture media, the heating mechanism 3 is started to heat the storage box 1, or people start the refrigerating mechanism 4 to refrigerate the storage box 1, the temperature condition of the culture media is met by adjusting the temperature of the storage box 1, outside air enters the air inlet box 5 through the first screen plate 6, the environment in the culture media cannot be damaged after the air is filtered and sterilized through the filter layer 7 and the sterilizing layer 8, then the air enters the heating mechanism 3 or the refrigerating mechanism 4 through the third air pump 9 and the air inlet pipe 10, the second screen plate 35 can exhaust air to ensure the air pressure balance in the storage box 1, when the culture medium needs to be observed, people only need to take out the culture medium through the glass door 2.
Because the heating mechanism 3 comprises the heating box 301, the shell 302, the heating wire 303, the first air pump 304, the first air pipe 305, the first air outlet pipe 306 and the first spray head 307, the heating box 301 is arranged on the left side of the top of the storage box 1, the left air inlet pipe 10 penetrates through the top of the heating box 301, the shell 302 is arranged on the top and the inner bottom of the heating box 301, the heating wire 303 is arranged in the shell 302, the first air pump 304 is arranged on the left side of the inner bottom of the heating box 301, the first air pipe 305 is arranged on the first air pump 304, the first air outlet pipe 306 is arranged on the lower portion of the first air pipe 305, the first air outlet pipe 306 penetrates through the top of the storage box 1, the first spray head 307 is uniformly arranged on the right side of the first air outlet pipe 306, when the storage box 1 needs to be heated, people start the heating wire 303 to heat the air entering the heating box 301, then start the first air pump 304 to convey the air into the first, so that the temperature in the storage box 1 rises, and the heating wire 303 and the first air pump 304 are closed after the temperature reaches the temperature required by the culture medium.
Because the refrigerating mechanism 4 comprises the refrigerating box 41, the refrigerator 42, the second air pump 43, the second air pipe 44, the second air outlet pipe 45 and the second spray head 46, the refrigerating box 41 is arranged on the right side of the top of the storage box 1, the right air inlet pipe 10 penetrates through the top of the refrigerating box 41, the refrigerator 42 is arranged on the top of the refrigerating box 41, the second air pump 43 is arranged on the right side of the inner bottom of the refrigerating box 41, the second air pipe 44 is arranged on the second air pump 43, the second air outlet pipe 45 is arranged on the lower portion of the second air pipe 44, the second air outlet pipe 45 penetrates through the top of the refrigerating box 41, the second spray head 46 is uniformly arranged on the left side of the first air outlet pipe 306, when the storage box 1 needs to be refrigerated, people start the refrigerator 42 to refrigerate the air entering the refrigerating box 41, then start the second air pump 43 to convey the air into the second air pipe 44 and the second air outlet pipe 45, and spray the cold, after the temperature of the culture medium is reached, the refrigerator 42 and the second air pump 43 are turned off.
Because the device also comprises a second support rod 14, a first slide rail 15, a bearing seat 16, an electric reel 17, a pull rope 18, a first slide block 19, a rack 20, a gear 21 and a third support rod 34, the second support rod 14 and the first slide rail 15 are arranged at the bottom in the storage box 1, the first slide rail 15 is positioned between the second support rods 14 at the left and right sides, the bearing seats 16 are symmetrically arranged at the top and the inner bottom in the storage box 1, the bearing seats 16 are positioned in front of the first slide rail 15, the third support rod 34 is arranged between the bearing seats 16 at the upper and lower sides, the placing frame 13 is arranged on the third support rod 34, the gear 21 is arranged at the lower part of the third support rod 34, the electric reel 17 is arranged at the upper ends of the second support rods 14 at the left and right sides, the pull rope 18 is wound on the electric reel 17, the first slide blocks 19 are symmetrically arranged at the left and right sides of the top of the first slide rail 15, the left, the rack 20 is located behind the gear 21, the rack 20 is meshed with the gear 21, so when the culture medium needs to be heated, people can start the left electric reel 17 to release the pull rope 18, the right electric reel 17 to release the pull rope 18, thereby driving the rack 20 to move rightwards, further enabling the gear 21 to drive the culture medium on the placing rack 13 to rotate, when the culture medium rotates to a position close to the first nozzle 307, people can close the electric reel 17, thereby the culture medium can be heated to the required temperature rapidly, when the culture medium needs to be heated, people can start the left electric reel 17 to release the pull rope 18, thereby driving the rack 20 to move leftwards, further enabling the gear 21 to drive the culture medium on the placing rack 13 to rotate, when the culture medium rotates to a position close to the second nozzle 46, people can close the electric reel 17, thereby rapidly cooling the culture medium to the required temperature, this prevents the environment in the medium from changing due to the long heating or cooling time.
Because the culture medium taking device further comprises a first supporting plate 22, a second sliding rail 23, a second sliding block 24, a drawer 25 and a handle 27, the first supporting plate 22 is uniformly arranged on a third supporting rod 34, the second sliding rail 23 is symmetrically arranged on the left side and the right side of the top of the first supporting plate 22, the second sliding block 24 is arranged on the second sliding rail 23, the drawer 25 is arranged between the second sliding blocks 24 on the left side and the right side, a groove 26 is formed in the middle of the drawer 25, the third supporting rod 34 penetrates through the groove 26, and the handle 27 is arranged on the front portion of the drawer 25, people can pull out the drawer 25 through the handle 27 and then place the culture medium on the drawer 25, and then people push back and reset the drawer 25, the drawer 25 can be pulled and pulled by the second sliding rail 23 and the second sliding block 24 more easily, when people need to take out the culture medium, the operation is repeated, and people can take and.
Because the culture box further comprises a second supporting plate 28, a placing frame 29 and a drying agent 30, the second supporting plate 28 is arranged on the left side of the lower portion of the third supporting rod 34, the second supporting plate 28 is located above the gear 21, the placing frame 29 is arranged on the top of the second supporting plate 28, and the drying agent 30 is arranged in the placing frame 29, the drying agent 30 can dry the storage box 1, and the storage of a culture medium is prevented from being influenced due to the fact that bacteria are bred in the storage box 1 due to excessive humidity.
Because the temperature gauge 31, the temperature sensor 32 and the controller 33 are further included, the temperature gauge 31 is arranged on the left side of the rear part of the glass door 2, the temperature sensor 32 and the controller 33 are arranged on the right side of the rear part of the glass door 2, the temperature sensor 32 is positioned above the controller 33, and the temperature sensor 32 is connected with the controller 33, people can observe the temperature gauge 31 through the glass door 2 so as to know the temperature value in the storage box 1, because the culture medium needs to be stored at a proper temperature, when the temperature sensor 32 detects that the temperature in the storage box 1 is lower than the preset value, the temperature sensor 32 feeds back the detected signal to the controller 33, the controller 33 controls the heating mechanism 3 to work so as to heat the storage box 1, and when the temperature sensor 32 detects that the temperature in the storage box 1 is higher than the preset value, the temperature sensor 32 feeds back the detected signal to the, the controller 33 controls the operation of the cooling mechanism 4 so that the storage box 1 is cooled.
The controller 33 selects a PLC controller 33 of model S24S0T, which is produced by Xiamenhai science and technology limited, and has 24VDC power supply, 16 input channels and 8 output channels outside the host. The definition of input-output port is: x0 is on, X1 is off, and X2 is temperature sensor 32; y0 is heating wire 303, Y1 is first air pump 304, Y2 is refrigerator 42, Y3 is second air pump 43, Y4 is third air pump 9, and Y5 is electric reel 17. According to the electrical control schematic diagram shown in fig. 10, those skilled in the art can control each component to operate according to the above-mentioned operation principle by programming without creative labor, and related instructions of programming are prior art and will not be described herein again.
The control mode of the present invention is automatically controlled by the controller 33, the control circuit of the controller 33 can be realized by simple programming by those skilled in the art, which belongs to the common knowledge in the field, and the present invention is mainly used for protecting the mechanical device, so the control mode and the circuit connection are not explained in detail in the present invention.
The above examples are merely representative of preferred embodiments of the present invention, and the description thereof is more specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, various changes, modifications and substitutions can be made without departing from the spirit of the present invention, and these are all within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (4)

1. A temperature-adjustable culture medium storage device for biology is characterized by comprising a storage box (1), a glass door (2), a heating mechanism (3), a refrigerating mechanism (4), an air inlet box (5), a first screen plate (6), a filter layer (7), a sterilization layer (8), a third air pump (9), an air inlet pipe (10), a one-way valve (11), a first support rod (12), a placing frame (13) and a second screen plate (35), wherein the glass door (2) is arranged at the front part of the storage box (1), the heating mechanism (3) and the refrigerating mechanism (4) are arranged at the top part of the storage box (1), the heating mechanism (3) is positioned at the left side of the refrigerating mechanism (4), the air inlet box (5) is arranged at the top parts of the heating mechanism (3) and the refrigerating mechanism (4), the first screen plate (6) is arranged at the top part of the air inlet box (5), the filter layer (7) and the sterilization layer (8) are arranged between, the filter layer (7) is positioned above the sterilization layer (8), a third air pump (9) is arranged in the middle of the bottom in the air inlet box (5), air inlet pipes (10) are symmetrically arranged on the left side and the right side of the third air pump (9), check valves (11) are arranged on the lower portions of the left air inlet pipe (10) and the right air inlet pipe (10), a first supporting rod (12) is arranged between the upper wall and the lower wall in the storage box (1), a placing frame (13) is uniformly arranged on the first supporting rod (12), and a second screen plate (35) is arranged on the right side of the bottom of; the heating mechanism (3) comprises a heating box (301), a shell (302), an electric heating wire (303), a first air pump (304), a first air pipe (305), a first air outlet pipe (306) and a first spray head (307), the left side of the top of the storage box (1) is provided with the heating box (301), the left air inlet pipe (10) penetrates through the top of the heating box (301), the shells (302) are arranged at the inner top and the inner bottom of the heating box (301), the electric heating wire (303) is arranged in the shell (302), the left side of the inner bottom of the heating box (301) is provided with the first air pump (304), the first air pipe (305) is arranged on the first air pump (304), the first air outlet pipe (306) is arranged at the lower part of the first air pipe (305), the first air outlet pipe (306) penetrates through the top of the storage box (1), and the first spray head (; the refrigerating mechanism (4) comprises a refrigerating box (41), a refrigerator (42), a second air pump (43), a second air pipe (44), a second air outlet pipe (45) and a second spray head (46), the refrigerating box (41) is arranged on the right side of the top of the storage box (1), the right air inlet pipe (10) penetrates through the top of the refrigerating box (41), the refrigerator (42) is arranged on the top of the refrigerating box (41), the second air pump (43) is arranged on the right side of the bottom of the refrigerating box (41), the second air pipe (44) is arranged on the second air pump (43), the second air outlet pipe (45) is arranged on the lower portion of the second air pipe (44), the second air outlet pipe (45) penetrates through the top of the refrigerating box (41), and the second spray head (46) is uniformly arranged on the left side of the; the electric reel device is characterized by further comprising a second supporting rod (14), a first sliding rail (15), a bearing seat (16), an electric reel (17), a pull rope (18), a first sliding block (19), a rack (20), a gear (21) and a third supporting rod (34), wherein the second supporting rod (14) and the first sliding rail (15) are arranged at the bottom in the storage box (1), the first sliding rail (15) is positioned between the left and right second supporting rods (14), the bearing seats (16) are symmetrically arranged at the top and the bottom in the storage box (1), the bearing seats (16) are positioned in front of the first sliding rail (15), the third supporting rod (34) is arranged between the upper and lower bearing seats (16), the placing frame (13) is arranged on the third supporting rod (34), the gear (21) is arranged at the lower part of the third supporting rod (34), the electric reels (17) are arranged at the upper ends of the left and right second supporting rods (14), and the pull rope (18) is, first slider (19) are symmetrically arranged on the left side and the right side of the top of the first sliding rail (15), the left side and the right side of the first slider (19) are connected with a pull rope (18), a rack (20) is arranged on the top of the first slider (19) on the left side and the right side, the rack (20) is located behind a gear (21), and the rack (20) is meshed with the gear (21).
2. The temperature-adjustable culture medium storage device for organisms according to claim 1, further comprising a first support plate (22), a second slide rail (23), a second slide block (24), a drawer (25) and a handle (27), wherein the first support plate (22) is uniformly arranged on the third support rod (34), the second slide rail (23) is symmetrically arranged on the left side and the right side of the top of the first support plate (22), the second slide block (24) is arranged on the second slide rail (23), the drawer (25) is arranged between the left side and the right side of the second slide block (24), a groove (26) is formed in the middle of the drawer (25), the third support rod (34) penetrates through the groove (26), and the handle (27) is arranged at the front part of the drawer (25).
3. The temperature-adjustable culture medium storage device for the organisms as claimed in claim 2, further comprising a second support plate (28), a placement frame (29) and a desiccant (30), wherein the second support plate (28) is arranged on the left side of the lower portion of the third support rod (34), the second support plate (28) is located above the gear (21), the placement frame (29) is arranged on the top of the second support plate (28), and the desiccant (30) is arranged in the placement frame (29).
4. The temperature-adjustable biological culture medium storage device according to claim 3, further comprising a thermometer (31), a temperature sensor (32) and a controller (33), wherein the thermometer (31) is arranged on the left side of the rear part of the glass door (2), the temperature sensor (32) and the controller (33) are arranged on the right side of the rear part of the glass door (2), the temperature sensor (32) is positioned above the controller (33), and the temperature sensor (32) is connected with the controller (33).
CN201710742097.7A 2017-08-25 2017-08-25 Equipment is deposited with culture medium of adjustable temperature to biology Active CN107446814B (en)

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CN107828630B (en) * 2017-12-13 2021-03-09 李燕 Medical microbial cultivation device
CN114350513A (en) * 2021-12-28 2022-04-15 艾美康淮生物制药(江苏)有限公司 Cell subculture batch operation device and method

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CN205803490U (en) * 2016-06-08 2016-12-14 天津绿持能源技术有限公司 A kind of aerobic microbiological installation for fermenting
CN205934590U (en) * 2016-07-23 2017-02-08 湖北金卫康防护用品有限公司 Make production facility that bonded fabric is smooth fine and smooth
CN206396220U (en) * 2017-01-25 2017-08-11 新疆医科大学附属肿瘤医院 A kind of electric heating constant temperature culture apparatus suitable for cell research

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Publication number Priority date Publication date Assignee Title
CN205329060U (en) * 2016-02-02 2016-06-22 河南科技学院 Microbial cultivation box
CN205803490U (en) * 2016-06-08 2016-12-14 天津绿持能源技术有限公司 A kind of aerobic microbiological installation for fermenting
CN106171315A (en) * 2016-06-30 2016-12-07 哈尔滨尼亚农业有限公司 A kind of Multi-function seed storage facilities
CN205934590U (en) * 2016-07-23 2017-02-08 湖北金卫康防护用品有限公司 Make production facility that bonded fabric is smooth fine and smooth
CN206396220U (en) * 2017-01-25 2017-08-11 新疆医科大学附属肿瘤医院 A kind of electric heating constant temperature culture apparatus suitable for cell research

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