CN109588604A - A kind of pinctada fucata glycosaminoglycan effervescent tablet and preparation method thereof - Google Patents
A kind of pinctada fucata glycosaminoglycan effervescent tablet and preparation method thereof Download PDFInfo
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- CN109588604A CN109588604A CN201811344841.9A CN201811344841A CN109588604A CN 109588604 A CN109588604 A CN 109588604A CN 201811344841 A CN201811344841 A CN 201811344841A CN 109588604 A CN109588604 A CN 109588604A
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- Prior art keywords
- glycosaminoglycan
- pinctada fucata
- effervescent tablet
- pinctada
- preparation
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Classifications
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- A—HUMAN NECESSITIES
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- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
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- A—HUMAN NECESSITIES
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- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/40—Effervescence-generating compositions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0066—Isolation or extraction of proteoglycans from organs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention relates to a kind of pinctada fucata glycosaminoglycan effervescent tablets and preparation method thereof, include following components in percentage by weight: pinctada fucata glycosaminoglycan 15%-25%, disintegrating agent 30%-40%, adhesive 1%-3%, lubricant 1%-3%, corrigent 2%-4%, surplus are lactose.Pinctada fucata glycosaminoglycan effervescent tablet piece type prepared by the present invention is that biplane is round, shape is clean and tidy, surface is smooth, and color is white, uniformity, meets water and is disintegrated rapidly and generates bulk gas, mouthfeel is soft, free from admixture, has distinctive fragrance and flavour, and organoleptic quality is good, it has no toxic side effect, helps to maintain the normal immune function of body.The method of the present invention process operability is strong, low in cost, is suitble to industrialized production, provides technological innovation for the application of pinctada fucata glycosaminoglycan and the high-valued exploitation of shellfish meat, significant to the development of promotion pearl aquaculture and processing industry.
Description
Technical field
The present invention relates to health food processing technique field, in particular to a kind of pinctada fucata glycosaminoglycan effervescent tablet and
Preparation method.
Background technique
Pinctada fucata Pinctada fucata martensii (Dunker) also known as pteria martensii, are south Chinas
The principal item of seawater pearl culture is distributed in Guangdong, Guangxi and Hainan coastal area.Pinctada fucata is in addition to being rich in high-quality egg
Except white matter and several mineral materials, glycosaminoglycan in the middle is also especially noticeable.Glycosaminoglycan
(Glycosaminoglycans, GAGs), also referred to as glycosaminoglycan or acid mucopolysaccharide are the length being made of duplicate disaccharide unit
Chain polysaccharide is present in the tissue of most marine animals, and structure and property are different from known terrestrial plant polysaccharide, are reported
Road has a variety of effects such as enhancing immune function, anticoagulation, hypoglycemic, antitumor.According to the literature, glycosaminoglycan can stimulate machine
Maturation, differentiation and the breeding of the various immunocompetent cells (Dendritic Cells, bone-marrow-derived lymphocyte) of body increase the non-spy of macrophage
Specific cell poison, the generation of cell factors and the tables of cytokine receptor such as induction IL-1, IL-2, TNF-α, IFN-γ, IL-8
It reaches, antigen submission, antibody is promoted to be formed, the effects of the classical pathway and change approach of complement activation system.Pinctada fucata sugar
Amine glycan is a kind of natural bioactive polysaccharide with immunoloregulation function, mainly by D- Glucosamine, D- ammonia
The monosaccharide such as base glucuronic acid, glucose, mannose, galactolipin, xylose, fucose are constituted.
After pearl is adopted in pearl agriculture, annual remaining about 3500-4000 tons of processing byproduct pearl oyster meat, is mostly fresh or thick
Eaten after processing, sold with fresh goods based on, it is cheap (about 5-10 member/kg), in Guangdong Leizhou City and Xuwen County, Beihai Fisheries Base Guangxi Province
The ground such as city, Hainan cause the huge waste of resource, and deep processing rate is lower, and preservation term is short, easily putrid and deteriorated, lead to pearl oyster meat
Development and utilization are restricted.
As 103039792A patent application discloses " it is a kind of for enhance immune glycosaminoglycan from Pinctada martensii soft capsule and its
Preparation method ", technique are that nacre whole viscera homogenate enzymatic hydrolysis, centrifugation alcohol precipitation, dry the glycosaminoglycan of washing and are added auxiliary
Soft capsule is made in material.But the defect of the above method is: before pearl oyster meat is without adding flooding sofening treatment, through testing
Card, thick glycosaminoglycan purity obtained is only 30%-45%, the too low immunoenhancement result that will affect obtained product of purity.
Effervescent tablet is a kind of tablet containing Effervescent tablet disintegration agent.Disintegrating agent is usually organic acid and sodium carbonate, bicarbonate
The mixture of sodium, into the water after, acid-base reactions occur for two kinds of substances, generate great amount of carbon dioxide, make tablet be disintegrated rapidly with
Melt, the bubble for being disintegrated generation makes tablet rolling up and down in water, accelerates it to be disintegrated and melt, is partially dissolved in drinking-water, makes
When drinking-water is drunk in entrance, there is the aesthetic feeling as carbonated drink, liked by the current young consumer group, is a kind of novel solid drink
Material.Effervescent tablet originates in pharmaceutical field, has many advantages, such as that rapid-action, utilization rate is high, easy to carry, at present to field of food
Development, such as pumpkin corn powder effervescent tablet, hawthorn effervescent tablet, effervescent tea tablet.Under the premise of pinctada fucata resource complete utilization
Pinctada fucata glycosaminoglycan effervescent tablet is developed, it can be maximum while effectively supplement pinctada fucata glycosaminoglycan function factor
The extra large fishy smell for reducing to limit pinctada fucata shellfish meat itself, enhances agreeable to the taste sense, for people healthy living provide it is a kind of novel
Pinctada fucata glycosaminoglycan health food.
Summary of the invention
The first purpose of the invention is to provide a kind of pinctada fucata glycosaminoglycan effervescent tablet, can help to remain normal
Immune function is convenient for carrying, and breaches traditional limitation of existing pinctada fucata food development form, and solves Hepu pearl oyster
The lower problem of shellfish shellfish meat higher value application rate.
A second object of the present invention is to provide the preparation method of above-mentioned pinctada fucata glycosaminoglycan effervescent tablet, this method
The product of being made purity is high, it is simple and easy, it is suitble to industrialized production.
Solving technical solution used by first purpose of the invention is:
A kind of pinctada fucata glycosaminoglycan effervescent tablet, it includes following weight percent meter components:
The disintegrating agent is sodium bicarbonate and citric acid, preferably, the proportion of the sodium bicarbonate and citric acid is 2:
1。
Described adhesive is 10% polyvinylpyrrolidone ethanol solution.Because experiment uses soda acid mixing granulation technique, need
Strict control contact wetting, so the polyvinylpyrrolidone (PVP) that selection dissolves in dehydrated alcohol makees adhesive
The lubricant is PEG4000.Since mobility of particle is bad when to avoid film-making, there is slice weight fluctuation greatly, out
Lubricant will be added in phenomenons, the particles such as piece is difficult, finish is low before tabletting.Soluble oil PEG 4000 collapses effervescent tablet
Clarity after solution is without influence, and lubricity and resistance to bond are preferable, preferably select
The corrigent includes 0.1% essence, and surplus is sweetener, preferably, the sweetener is Sucralose.
Solving technical solution used by second purpose of the invention is:
A kind of preparation method of pinctada fucata glycosaminoglycan effervescent tablet comprising following steps:
S1, add flooding after taking pinctada fucata meat tissue to be homogenized, adjust pH to 7.0-7.6, add neutral proteinase and pancreas
Protease composite enzyme is digested, centrifuging and taking supernatant after enzyme deactivation, obtains pinctada fucata glycosaminoglycan mother liquor；
S2, gained pinctada fucata glycosaminoglycan mother liquor is subjected to alcohol precipitation processing, precipitating is filtered and washed after centrifugation, freezed
Dry thick glycosaminoglycan；
S3, thick glycosaminoglycan is taken, after anion exchange chromatography, dialysis concentration is freeze-dried to obtain pinctada fucata sugar
Amine glycan；
S4, it screens, then sprays after taking pinctada fucata glycosaminoglycan and disintegrating agent, diluent to be mixed evenly in proportion
Adhesive softwood is granulated, and is added corrigent, lubricant, after mixing after dry, whole grain, compression molding, after sterilization packaging
Obtain pinctada fucata glycosaminoglycan effervescent tablet finished product.In the present invention, diluent is lactose.
Further, the solid-liquid ratio that flooding is added in the step S1 is 1:2,45-55 DEG C under magnetic stirring apparatus effect
Heating water bath 4-6h.
The pH of 0.1mol/L sodium hydrate regulator solution is used in the step S1.
The amount of neutral proteinase and trypsase complex enzyme is that 10- is added in every gram of pinctada fucata shellfish meat in the step S1
The mass ratio of 15mg, neutral proteinase and trypsase is 7:8.
In the step S1, enzymatic hydrolysis 45-55 DEG C of heating water bath 4h under magnetic stirring apparatus effect.
Destroy the enzyme treatment is the inactivated proteases 10min in 100 DEG C of water-baths in the step S1, is cooled to room temperature；It is described from
Heart processing is that 8000-12000rpm is centrifuged 15-20min.
Further, alcohol precipitation processing is in the step S2 plus dehydrated alcohol is to final concentration 75%, precipitates 8-12h at 4 DEG C.
Centrifugation is with 10000-12000rpm centrifugation 15-20min in the step S2；Washing is precipitated as with dehydrated alcohol-
Acetone alternately washing 4-5 times.
Freeze-drying is in 0.030~0.035kPa, -50~-55 DEG C of vacuum freezedrying 12-36h in the step S2.
Further, anion exchange chromatography is using DEAE-52 anion-exchange column to raw sugar in the step S3
Amine glycan is purified, applied sample amount 10mL, and flow velocity 1mL/min, every pipe collects 10mL, and mobile phase is respectively pure water, 0.5mol/
mLNaAc、1.5mol/mLNaCl。
Dialysis concentration is to be dialysed for 24 hours with the bag filter of molecular cut off 8KD-14KD in the step S3.
Freeze-drying is in 0.030~0.035kPa, -50~-55 DEG C of vacuum freezedrying 12-36h in the step S3.
Further, in the step S4 screening to sieve with 100 mesh sieve, be granulated for 16 meshes, whole grain be 20 meshes.
Drying is dried to moisture content lower than 0.5% in the step S4 under the conditions of being 60-70 DEG C.
Sterilizing is using ultraviolet disinfecting machine progress ultraviolet-sterilization in the step S4.
The mesh number requirement of step S4, has an impact to tablet molding and fater disintegration.
The beneficial effects of the present invention are:
1) before carrying out enzymolysis processing, hot water extraction first is carried out to raw material, volume of material is expanded, makes albumen in raw material
The prototype structure part of matter changes, and can save enzymolysis time, save foreign protein in enzyme dosage, more preferably removing finished product.
2) anion exchange chromatography, bag filter dialysis purification glycosaminoglycan is added in the present invention, reaches obtained product purity
85% or more, there is huge promotion compared to the purification purity without extraction, is a kind of glycosaminoglycan extraction of raising to greatest extent
The new process of rate.
3) pinctada fucata glycosaminoglycan effervescent tablet prepared by the present invention is biplane circular piece type, and surface is smooth, color
White, uniformity meets water and is disintegrated and generates bulk gas rapidly, has distinctive fragrance and flavour, and action temperature and nothing
Toxic side effect has apparent health-care efficacy, helps to maintain the normal immune function of human organism.
4) preparation method is simple for pinctada fucata glycosaminoglycan effervescent tablet of the present invention, is suitble to industrialized production, favorably
In solving the problems, such as pinctada fucata higher value application, the wasting of resources is avoided, while solving environmental pollution caused by shellfish meat abandons
Problem promotes the sustainable and healthy development of pearl aquaculture and secondary industry.
Detailed description of the invention
Fig. 1 is histogram of the pinctada fucata glycosaminoglycan effervescent tablet to the protective effect of spleen；
Fig. 2 is the histogram of the effect of pinctada fucata glycosaminoglycan effervescent tablet Number of Peripheral Blood Leucocyte；
Fig. 3 is histogram of the pinctada fucata glycosaminoglycan effervescent tablet to the effect of bone marrow cell；
Fig. 4 is histogram of the pinctada fucata glycosaminoglycan effervescent tablet to the effect of spleen lymphocyte proliferation ability；
Fig. 5 is histogram of the pinctada fucata glycosaminoglycan effervescent tablet to the effect of spleen NK cell activity；
Fig. 6 is histogram of the pinctada fucata glycosaminoglycan effervescent tablet to the effect of peritoneal macrophage phagocytic activity；
Fig. 7 is histogram of the pinctada fucata glycosaminoglycan effervescent tablet to the effect of serum IL-2 level.
Specific embodiment
Technical solution of the present invention is described further with reference to the accompanying drawings and examples, so that those skilled in the art
The present invention can be better understood and can be practiced, but illustrated embodiment is not as a limitation of the invention.
Embodiment 1
A kind of pinctada fucata glycosaminoglycan effervescent tablet for helping to maintain normal immunological function, the effervescent tablet slice weight are
202.3mg ± 2.8mg comprising pinctada fucata glycosaminoglycan 25%, disintegrating agent 36%, adhesive 2.5%, lubricant 2%, is rectified
Taste agent 3%, surplus are lactose.
The preparation method of pinctada fucata glycosaminoglycan effervescent tablet in the present embodiment, comprising the following steps:
(1) pinctada fucata meat tissue 2568g is taken, flooding is added for 1:2 with solid-liquid ratio after homogenate, is made in magnetic stirring apparatus
With lower 50 DEG C of water bath with thermostatic control 5h, with the pH to 7.6 of the sodium hydrate regulator solution of 0.1mol/L；With every gram of pinctada fucata shellfish meat
15mg neutral proteinase and trypsase combined hydrolysis (mass ratio of neutral proteinase and trypsase is 7:8) is added, in magnetic
Power blender acts on lower 50 DEG C of water-bath 4h.After in 100 DEG C of water-baths inactivated proteases 10min, be cooled to room temperature,
11000rpm is centrifuged 15min, collects supernatant, obtains pinctada fucata glycosaminoglycan mother liquor；
(2) add dehydrated alcohol to final concentration 75% in gained pinctada fucata glycosaminoglycan mother liquor, precipitate 8h at 4 DEG C, then
It is centrifuged 15min with 11000rpm, precipitating dehydrated alcohol-acetone alternately washing 4 times, in 0.032kPa, -52 DEG C of freezing vacuums are dry
Dry to obtain thick glycosaminoglycan 33.51g for 24 hours, crude product yield is 1.30%；
(3) the thick glycosaminoglycan for taking step (2) purifies thick glycosaminoglycan using DEAE-52 anion-exchange column,
Applied sample amount 10mL, flow velocity 1mL/min, every pipe collect 10mL, and mobile phase is respectively pure water, 0.5mol/mLNaAc, 1.5mol/
MLNaCl is dialysed for 24 hours with the bag filter of molecular cut off 10KD, is closed in 0.032kPa, -52 DEG C of vacuum freezedrying 28h
Pu glycosaminoglycan from Pinctada martensii 13.47g, highly finished product yield are 0.525%, and purity is up to 86.9%；
(4) 100 mesh are crossed after pinctada fucata glycosaminoglycan and disintegrating agent, lactose being mixed evenly according to the above ratio
Sieve, the polyvinylpyrrolidone ethanol solution of mass concentration 10% is slowly sprayed to mixed raw material, while constantly stirring by 2mL/10g
It mixes uniformly, neither too hard, nor too soft softwood is made, cross 16 meshes, so that softwood is become particle, manufactured wet granular is spread in enamel tray
In, it is dried to moisture content under the conditions of 65 DEG C lower than 0.5% hereinafter, being crushed with pulverizer, crosses 20 meshes, mass fraction is added
2% lubricant and 3% corrigent carry out ultraviolet-sterilization using ultraviolet disinfecting machine, using double with single-punch tablet press tabletting
Pinctada fucata glycosaminoglycan effervescent tablet 210, slice weight 202.3mg is made by the specification packaging of 10 every plates in aluminium packaging altogether
± 2.8mg, the every 50.58mg of glycosaminoglycan containing pinctada fucata.
According to the regulation of GB 16740-2014 national food safety standard health food, gained final product quality index such as table 1
It is shown, meet the requirements.
The detection of 1 pinctada fucata glycosaminoglycan effervescent tablet quality index of table
Embodiment 2
A kind of pinctada fucata glycosaminoglycan effervescent tablet for helping to maintain normal immunological function, the effervescent tablet slice weight are
198.6mg ± 2.4mg includes pinctada fucata glycosaminoglycan 20%, disintegrating agent 33%, adhesive 2%, lubricant 2%, flavoring
Agent 3%, surplus are lactose.
The preparation method of pinctada fucata glycosaminoglycan effervescent tablet in the present embodiment, comprising the following steps:
(1) pinctada fucata meat tissue 2714g is taken, flooding is added for 1:2 with solid-liquid ratio after homogenate, is made in magnetic stirring apparatus
With lower 55 DEG C of water bath with thermostatic control 4h, with the pH to 7.2 of the sodium hydrate regulator solution of 0.1mol/L；With every gram of pinctada fucata shellfish meat
12mg neutral proteinase and trypsase combined hydrolysis (mass ratio of neutral proteinase and trypsase is 7:8) is added, in magnetic
Power blender acts on lower 55 DEG C of water-bath 4h.After in 100 DEG C of water-baths inactivated proteases 10min, be cooled to room temperature,
10000rpm is centrifuged 20min, collects supernatant, obtains pinctada fucata glycosaminoglycan mother liquor；
(2) add dehydrated alcohol to final concentration 75% in gained pinctada fucata glycosaminoglycan mother liquor, precipitate 12h at 4 DEG C, then
It is centrifuged 20min with 10000rpm, precipitating dehydrated alcohol-acetone alternately washing 4 times, in 0.031kPa, -54 DEG C of freezing vacuums are dry
Dry 30h obtains thick glycosaminoglycan 40.31g, and crude product yield is 1.49%, and purity is up to 89.2%；
(3) the thick glycosaminoglycan for taking step (2) purifies thick glycosaminoglycan using DEAE-52 anion-exchange column,
Applied sample amount 10mL, flow velocity 1mL/min, every pipe collect 10mL, and mobile phase is respectively pure water, 0.5mol/mLNaAc, 1.5mol/
MLNaCl is dialysed for 24 hours with the bag filter of molecular cut off 12KD, and in 0.032kPa, -51 DEG C of vacuum freezedryings are closed for 24 hours
Pu glycosaminoglycan from Pinctada martensii 16.19g, highly finished product yield are 0.600%；
(4) 100 mesh are crossed after pinctada fucata glycosaminoglycan and disintegrating agent, lactose being mixed evenly according to the above ratio
Sieve, the polyvinylpyrrolidone ethanol solution of mass concentration 10% is slowly sprayed to mixed raw material, while constantly stirring by 2mL/10g
It mixes uniformly, neither too hard, nor too soft softwood is made, cross 16 meshes, so that softwood is become particle, manufactured wet granular is spread in enamel tray
In, it is dried to moisture content under the conditions of 65 DEG C lower than 0.5% hereinafter, being crushed with pulverizer, crosses 20 meshes, mass fraction is added
2% lubricant and 3% corrigent carry out ultraviolet-sterilization using ultraviolet disinfecting machine, using double with single-punch tablet press tabletting
Pinctada fucata glycosaminoglycan effervescent tablet 230, slice weight 198.6mg is made by the specification packaging of 10 every plates in aluminium packaging altogether
± 2.4mg, the every 39.72mg of glycosaminoglycan containing pinctada fucata.
According to the regulation of GB 16740-2014 national food safety standard health food, gained final product quality index such as table 2
It is shown, meet the requirements.
The detection of 2 pinctada fucata glycosaminoglycan effervescent tablet quality index of table
Embodiment 3
A kind of pinctada fucata glycosaminoglycan effervescent tablet for helping to maintain normal immunological function, the effervescent tablet slice weight are
205.1mg ± 4.0mg includes pinctada fucata glycosaminoglycan 20%, disintegrating agent 39%, adhesive 3%, lubricant 3%, flavoring
Agent 4%, surplus are lactose.
The preparation method of pinctada fucata glycosaminoglycan effervescent tablet in the present embodiment, comprising the following steps:
(1) pinctada fucata meat tissue 2618g is taken, flooding is added for 1:2 with solid-liquid ratio after homogenate, is made in magnetic stirring apparatus
With lower 50 DEG C of water bath with thermostatic control 6h, with the pH to 7.4 of the sodium hydrate regulator solution of 0.1mol/L；With every gram of pinctada fucata shellfish meat
10mg neutral proteinase and trypsase combined hydrolysis (mass ratio of neutral proteinase and trypsase is 7:8) is added, in magnetic
Power blender acts on lower 50 DEG C of water-bath 4h.After in 100 DEG C of water-baths inactivated proteases 10min, be cooled to room temperature,
12000rpm is centrifuged 15min, collects supernatant, obtains pinctada fucata glycosaminoglycan mother liquor；
(2) add dehydrated alcohol to final concentration 75% in gained pinctada fucata glycosaminoglycan mother liquor, precipitate 12h at 4 DEG C, then
It is centrifuged 15min with 12000rpm, precipitating dehydrated alcohol-acetone alternately washing 4 times, in 0.034kPa, -52 DEG C of freezing vacuums are dry
Dry 36h obtains thick glycosaminoglycan 38.11g, and crude product yield is 1.46%, and purity is up to 88.5%；
(3) the thick glycosaminoglycan for taking step (2) purifies thick glycosaminoglycan using DEAE-52 anion-exchange column,
Applied sample amount 10mL, flow velocity 1mL/min, every pipe collect 10mL, and mobile phase is respectively pure water, 0.5mol/mLNaAc, 1.5mol/
MLNaCl is dialysed for 24 hours with the bag filter of molecular cut off 10KD, is closed in 0.032kPa, -53 DEG C of vacuum freezedrying 20h
Pu glycosaminoglycan from Pinctada martensii 13.87g, highly finished product yield are 0.530%；
(4) 100 mesh are crossed after pinctada fucata glycosaminoglycan and disintegrating agent, lactose being mixed evenly according to the above ratio
Sieve, the polyvinylpyrrolidone ethanol solution of mass concentration 10% is slowly sprayed to mixed raw material, while constantly stirring by 2mL/10g
It mixes uniformly, neither too hard, nor too soft softwood is made, cross 16 meshes, so that softwood is become particle, manufactured wet granular is spread in enamel tray
In, it is dried to moisture content under the conditions of 70 DEG C lower than 0.5% hereinafter, being crushed with pulverizer, crosses 20 meshes, mass fraction is added
3% lubricant and 4% corrigent carry out ultraviolet-sterilization using ultraviolet disinfecting machine, using double with single-punch tablet press tabletting
Pinctada fucata glycosaminoglycan effervescent tablet 190, slice weight 205.1mg is made by the specification packaging of 10 every plates in aluminium packaging altogether
± 4.0mg, the every 41.02mg of glycosaminoglycan containing pinctada fucata.
According to the regulation of GB 16740-2014 national food safety standard health food, gained final product quality index such as table 3
It is shown, meet the requirements.
The detection of 3 pinctada fucata glycosaminoglycan effervescent tablet quality index of table
The adjusting of immune function is made in order to verify pinctada fucata glycosaminoglycan effervescent tablet obtained in above-described embodiment
With and its safety above-mentioned implementation observed by building immunological function repression mouse model and oral acute toxicity rat model
Influence and Acute oral tox-hty test of the product to immune organ, immunocyte and cell factor etc. are made in example.
According to glycosaminoglycan active evaluation test result early period and adult-mouse dose reduction formula, pinctada fucata sugar
The mouse effective dose of amine glycan is 0.25mg/d, is adult effective dose 100mg/d according to formula scales, is equivalent to two panels bubble
Rise the GAG content of piece.
Pinctada fucata glycosaminoglycan effervescent tablet obtained in embodiment 1 is selected, is constructed using routine experiment method immune
Function inhibitio Balb/c inbred mouse model (SPF grades, half male and half female, weight 16-18g), 60 Balb/c mouse are random
It is divided into 6 test groups, glycosaminoglycan effervescent tablet low dosage (0.125mg/d), middle dosage (0.25mg/d), high dose is respectively set
(0.5mg/d) three groups stomach-filling given the test agent 21 days, are seen totally using lentinan film (0.25mg/d) as positive controls
It examines and influence of the product to immune organ, immunocyte and cell factor etc. is made in example 1.
Test data result is as follows:
1) protective effect of the pinctada fucata glycosaminoglycan effervescent tablet to spleen
As shown in Figure 1, cyclophosphamide causes seriously to damage to mouse spleen compared with Normal group, cause spleen etc.
The weight saving of immune organ illustrates model construction success (P < 0.01).Compared with model group, lentinan film, Hepu pearl oyster
Shellfish glycosaminoglycan effervescent tablet low dose group (0.125mg/d), middle dose group (0.25mg/d) and high dose group (0.5mg/d) are right
Mouse spleen damage has improvement result (P < 0.05), illustrates lentinan film and pinctada fucata glycosaminoglycan effervescent tablet to ring phosphorus
Spleen atrophy caused by amide all has apparent improvement.Glycosaminoglycan effervescent tablet low dose group (0.125mg/d), middle dose
Dose-effect relationship (P < 0.05) is presented in amount group (0.25mg/d), high dose group (0.5mg/d), but compared with normal group, each group is not
It is horizontal (P < 0.01) to be restored to Normal group.
In Fig. 1: normal group of * P < 0.05and**P < 0.01vs., #P < 0.05and##P < 0.01vs. model group (n=10)
(same in following figure)
2) effect of pinctada fucata glycosaminoglycan effervescent tablet Number of Peripheral Blood Leucocyte
As shown in Fig. 2, cyclophosphamide seriously reduces mouse peripheral blood leucocyte level compared with normal group, illustrate mould
Type constructs successfully (P < 0.01).Compared with model group, lentinan film, pinctada fucata glycosaminoglycan effervescent tablet low dose group
(0.125mg/d), middle dose group (0.25mg/d), high dose group (0.5mg/d) are under mouse peripheral blood leucocyte level
Drop has improvement result (P < 0.05), illustrates that it can be such that peripheral white blood cells level restores, shows lentinan film, pinctada fucata
Glycosaminoglycan effervescent tablet plays a protective role for the reduction of leucocyte.Pinctada fucata glycosaminoglycan effervescent tablet low dose group
(0.125mg/d), middle dose group (0.25mg/d), high dose group (0.5mg/d) present dose-effect relationship (P < 0.05), but with it is normal
Group is compared, and it is horizontal (P < 0.05) that each group does not recover to Normal group.
3) effect of the pinctada fucata glycosaminoglycan effervescent tablet to bone marrow cell
As shown in figure 3, cyclophosphamide causes seriously to damage to bone marrow cells in mice compared with normal group, illustrate model structure
Build up function (P < 0.01).Compared with model group, lentinan film, pinctada fucata glycosaminoglycan effervescent tablet low dose group
(0.125mg/d), middle dose group (0.25mg/d), high dose group (0.5mg/d) have improvement to make bone marrow cells in mice damage
With (P < 0.01).Pinctada fucata glycosaminoglycan effervescent tablet low dose group (0.125mg/d), middle dose group (0.25mg/d), height
Dose-effect relationship (P < 0.05) is presented in dosage group (0.5mg/d), and compared with normal group, high dose group (0.5mg/d) has been restored to
It is normal to organize horizontal (P > 0.05).
4) effect of the pinctada fucata glycosaminoglycan effervescent tablet to spleen lymphocyte proliferation ability
As shown in figure 4, cyclophosphamide causes seriously to damage to mice spleen lymphocytes proliferation ability compared with normal group,
Illustrate model construction success (P < 0.01).Compared with model group, lentinan film, low dose of pinctada fucata glycosaminoglycan effervescent tablet
Amount group (0.125mg/d), middle dose group (0.25mg/d), high dose group (0.5mg/d) are to mice spleen lymphocytes proliferation energy
The reduction of power has improvement result (P < 0.05).Pinctada fucata glycosaminoglycan effervescent tablet low dose group (0.125mg/d), middle dosage
Dose-effect relationship (P < 0.05) is presented in group (0.25mg/d), high dose group (0.5mg/d), but compared with normal group, each group is not extensive
It is horizontal (P < 0.01) that Normal group is arrived again.
5) effect of the pinctada fucata glycosaminoglycan effervescent tablet to spleen NK cell killing activity
As shown in figure 5, cyclophosphamide causes seriously to damage to mice spleen NK cell killing activity compared with normal group, say
Bright model construction success (P < 0.01).Compared with model group, lentinan film, pinctada fucata glycosaminoglycan effervescent tablet low dosage
Group (0.125mg/d), middle dose group (0.25mg/d), high dose group (0.5mg/d) are to mice spleen NK cell killing activity
Reduction has improvement result (P < 0.01).Pinctada fucata glycosaminoglycan effervescent tablet low dose group (0.125mg/d), middle dose group
Dose-effect relationship (P < 0.05) is presented in (0.25mg/d), high dose group (0.5mg/d) group, but compared with normal group, each group is not extensive
It is horizontal (P < 0.01) that Normal group is arrived again.
6) effect of the pinctada fucata glycosaminoglycan effervescent tablet to peritoneal macrophage phagocytic activity
As shown in fig. 6, cyclophosphamide causes seriously to damage to Turnover of Mouse Peritoneal Macrophages phagocytic activity compared with normal group
Wound illustrates model construction success (P < 0.01).Compared with model group, lentinan film, pinctada fucata glycosaminoglycan effervescent tablet
Low dose group (0.125mg/d), middle dose group (0.25mg/d), high dose group (0.5mg/d) are to Turnover of Mouse Peritoneal Macrophages
The reduction of phagocytic activity has improvement result (P < 0.05).Pinctada fucata glycosaminoglycan effervescent tablet low dose group (0.125mg/d),
Dose-effect relationship (P < 0.05) is presented in middle dose group (0.25mg/d), high dose group (0.5mg/d), but compared with normal group, each group
It is horizontal (P < 0.01) that Normal group is not recovered to.
7) effect of the pinctada fucata glycosaminoglycan effervescent tablet to serum IL-2 level
As shown in fig. 7, cyclophosphamide causes seriously to damage to mice serum IL-2 level compared with normal group, illustrate mould
Type constructs successfully (P < 0.01).Compared with model group, lentinan film, pinctada fucata glycosaminoglycan effervescent tablet middle dose group
(0.25mg/d), high dose group (0.5mg/d) have improvement result (P < 0.01) to the reduction of mice serum IL-2 level.Hepu
Glycosaminoglycan from Pinctada martensii effervescent tablet low dose group (0.125mg/d), middle dose group (0.25mg/d), high dose group (0.5mg/d)
It presents dose-effect relationship (P < 0.05), but compared with normal group, it is horizontal (P < 0.01) that each group does not recover to Normal group.
8) conclusion
After three weeks by stomach-filling given the test agent, pinctada fucata glycosaminoglycan effervescent tablet is to immune suppression caused by cyclophosphamide
Dose-dependent positive correlation effect is presented in system, and middle dosage (0.25mg/d) is that effective dose (is people according to body surface area conversion
Effective dose be 100mg/d), specific manifestation are as follows: accelerate index and spleen index, peripheral white blood cell and bone marrow cell it is extensive
It is multiple, enhance spleen lymphocyte proliferation and conversion capability, spleen NK cell killing activity and peritoneal macrophage phagocytic activity, increases blood
Clear IL-2 is horizontal, while can also preferably improve the quality of life of mouse.Compared with lentinan film, pinctada fucata osamine is poly-
The immunoregulation effect of sugared effervescent tablet is suitable, works gentle, has improvement result to multiple indexs, be applicable to sub-health population
The raising of immunity of organisms.
Acute oral tox-hty test refers to that primary or the interior test repeatedly contaminated for 24 hours, the main half for measuring tested material cause
Dead amount or concentration, and observe the poisoning manifestations of experimental animal.In the test, pinctada fucata glycosaminoglycan effervescent tablet group, solvent
Each 20 SD rats of control group are giving tested material process and in the 14d observation period, rat expression behaviour, activity situation, breathing, appearance
Gesture etc. is showed no exception, no rats death.After zootomy, heart, liver, spleen, stomach, intestines, kidney and the life of rat are visually observed
The internal organs such as gland shape, color, size and ratio are grown, any exception is showed no, lesion does not occur for Rats Organs and Tissues.Rat weight is just
Often increase, the rat weight of pinctada fucata glycosaminoglycan effervescent tablet group is equal compared with solvent control group in each minute point
There was no significant difference (P > 0.05), as shown in table 4.Therefore, under the experimental condition, pinctada fucata glycosaminoglycan effervescent tablet pair
SD rat oral maximum tolerated dose (MTD) is higher than 15g/kg, and toxicity grading is nontoxic.
Table 4SD rat peroral acute toxicity tests weight result
It is not limiting the scope of the present invention with the above embodiment of the present invention, embodiments of the present invention are unlimited
In this, all this kind above content according to the present invention is not being departed from according to the ordinary technical knowledge and customary means of this field
Under the premise of the above-mentioned basic fundamental thought of the present invention, the modifications of other diversified forms that above structure of the present invention is made, replacement or
Change, should all fall within the scope and spirit of the invention.
Claims (20)
1. a kind of pinctada fucata glycosaminoglycan effervescent tablet, which is characterized in that include following weight percent meter component:
2. pinctada fucata glycosaminoglycan effervescent tablet according to claim 1, which is characterized in that the disintegrating agent is carbonic acid
The proportion of hydrogen sodium and citric acid, the sodium bicarbonate and citric acid is 2:1.
3. pinctada fucata glycosaminoglycan effervescent tablet according to claim 1, which is characterized in that the lubricant is
PEG4000。
4. pinctada fucata glycosaminoglycan effervescent tablet according to claim 1-3, which is characterized in that the bonding
Agent is 10% polyvinylpyrrolidone ethanol solution.
5. pinctada fucata glycosaminoglycan effervescent tablet according to claim 1, which is characterized in that the corrigent includes
0.1% essence, surplus are sweetener.
6. pinctada fucata glycosaminoglycan effervescent tablet according to claim 5, which is characterized in that the sweetener is three
Chlorine sucrose.
7. the preparation method of pinctada fucata glycosaminoglycan effervescent tablet according to claim 1-6, feature exist
In, comprising the following steps:
S1, add flooding after taking pinctada fucata meat tissue to be homogenized, adjust pH to 7.0-7.6, add neutral proteinase and tryptose
Enzyme complex enzyme is digested, centrifuging and taking supernatant after enzyme deactivation, obtains pinctada fucata glycosaminoglycan mother liquor；
S2, gained pinctada fucata glycosaminoglycan mother liquor is subjected to alcohol precipitation processing, precipitating is filtered and washed after centrifugation, be freeze-dried
Obtain thick glycosaminoglycan；
S3, take thick glycosaminoglycan, after anion exchange chromatography, dialysis concentration, be freeze-dried pinctada fucata osamine is poly-
Sugar；
S4, it is screened after taking pinctada fucata glycosaminoglycan and disintegrating agent, diluent to be mixed evenly in proportion, then sprays bonding
Agent softwood is granulated, and is added corrigent, lubricant, after mixing after dry, whole grain, compression molding, must be closed after sterilization packaging
Pu glycosaminoglycan from Pinctada martensii effervescent tablet finished product.
8. the preparation method of pinctada fucata glycosaminoglycan effervescent tablet according to claim 7, which is characterized in that the step
In rapid S1 plus the solid-liquid ratio of flooding is 1:2, the 45-55 DEG C of heating water bath 4-6h under magnetic stirring apparatus effect.
9. the preparation method of pinctada fucata glycosaminoglycan effervescent tablet according to claim 7 or 8, which is characterized in that institute
Stating the amount of neutral proteinase and trypsase complex enzyme in step S1 is that 10-15mg is added in every gram of pinctada fucata shellfish meat, described
The mass ratio of neutral proteinase and trypsase is 7:8.
10. the preparation method of pinctada fucata glycosaminoglycan effervescent tablet according to claim 9, which is characterized in that described
Enzymatic hydrolysis 45-55 DEG C of heating water bath 4h under magnetic stirring apparatus effect in step S1.
11. the preparation method of pinctada fucata glycosaminoglycan effervescent tablet according to claim 10, which is characterized in that described
Destroy the enzyme treatment is the inactivated proteases 10min in 100 DEG C of water-baths in step S1, is cooled to room temperature；The centrifugal treating is 8000-
12000rpm is centrifuged 15-20min.
12. the preparation method of pinctada fucata glycosaminoglycan effervescent tablet according to claim 7 or 11, which is characterized in that
Alcohol precipitation processing is in the step S2 plus dehydrated alcohol is to final concentration 75%, precipitates 8-12h at 4 DEG C.
13. the preparation method of pinctada fucata glycosaminoglycan effervescent tablet according to claim 12, which is characterized in that described
Centrifugation is with 10000-12000rpm centrifugation 15-20min in step S2；Washing is precipitated as alternately being washed with dehydrated alcohol-acetone
4-5 times.
14. the preparation method of pinctada fucata glycosaminoglycan effervescent tablet according to claim 13, which is characterized in that described
Freeze-drying is in 0.030~0.035kPa, -50~-55 DEG C of vacuum freezedrying 12-36h in step S2.
15. special according to the preparation method of the described in any item pinctada fucata glycosaminoglycan effervescent tablets of claim 7,11,14
Sign is that anion exchange chromatography is to carry out using DEAE-52 anion-exchange column to thick glycosaminoglycan in the step S3
Purifying, applied sample amount 10mL, flow velocity 1mL/min, every pipe collect 10mL, mobile phase be respectively pure water, 0.5mol/mLNaAc,
1.5mol/mLNaCl。
16. the preparation method of pinctada fucata glycosaminoglycan effervescent tablet according to claim 15, which is characterized in that described
Dialysis concentration is to be dialysed for 24 hours with the bag filter of molecular cut off 8KD-14KD in step S3.
17. the preparation method of pinctada fucata glycosaminoglycan effervescent tablet according to claim 16, which is characterized in that described
Freeze-drying is in 0.030~0.035kPa, -50~-55 DEG C of vacuum freezedrying 12-36h in step S3.
18. according to the preparation method of the described in any item pinctada fucata glycosaminoglycan effervescent tablets of claim 7,11,14,17,
It is characterized in that, in the step S4 screening to sieve with 100 mesh sieve, be granulated for 16 meshes, whole grain be 20 meshes.
19. the preparation method of pinctada fucata glycosaminoglycan effervescent tablet according to claim 18, which is characterized in that described
Drying is dried to moisture content lower than 0.5% in step S4 under the conditions of being 60-70 DEG C.
20. the preparation method of pinctada fucata glycosaminoglycan effervescent tablet according to claim 19, which is characterized in that described
Sterilizing is using ultraviolet disinfecting machine progress ultraviolet-sterilization in step S4.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1562073A (en) * | 2003-05-07 | 2005-01-12 | 汤毅 | Non-injection preparation containing medium and/or low molecular weight chondroitin sulfate |
| CN1947724A (en) * | 2006-03-10 | 2007-04-18 | 北京阜康仁生物制药科技有限公司 | Compound amino-glucose hydrochloride, chondroitin sulfate effervescent, tablets prepn. method and use thereof |
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2018
- 2018-11-13 CN CN201811344841.9A patent/CN109588604A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1562073A (en) * | 2003-05-07 | 2005-01-12 | 汤毅 | Non-injection preparation containing medium and/or low molecular weight chondroitin sulfate |
| CN1947724A (en) * | 2006-03-10 | 2007-04-18 | 北京阜康仁生物制药科技有限公司 | Compound amino-glucose hydrochloride, chondroitin sulfate effervescent, tablets prepn. method and use thereof |
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Application publication date: 20190409 |