CN109580821A - The detection method of impurity succinic acid in a kind of S- benzyl succinic acid - Google Patents
The detection method of impurity succinic acid in a kind of S- benzyl succinic acid Download PDFInfo
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Abstract
The invention discloses a kind of detection methods of impurity succinic acid in S- benzyl succinic acid.This method is qualitative and quantitative to succinic acid progress in S- benzyl succinic acid using HPLC detection method using Agilent SB-CN as chromatographic column, and has carried out methodology validation, the experiment proved that, this method has many advantages, such as that specificity is strong, quick, sensitive, accurate.The present invention establishes the qualitative and quantitative approach of impurity succinic acid in S- benzyl succinic acid for the first time, controls convenient for the quality to S- benzyl succinic acid, to improve the drug safety of the active constituent using S- benzyl succinic acid as intermediate.
Description
Technical field
The present invention relates to the impurity in a kind of S- benzyl succinic acid --- and the detection method of succinic acid belongs to medical science neck
Domain.
Background technique
S- benzyl succinic acid ((S) -2-Benzylsuccinic acid) is a kind of important medicine intermediate, main to use
In the production of Mitiglinide Calcium.Mitiglinide Calcium is by Japanese Kissei Pharmaceutical Co., Ltd. (KISSEI
PHARMACEUTICAL) the ATP dependent form potassium channel blocker developed, in April, 2004 list in Japan for the first time, and 2006
In Korean market, 2010 Nian Yuanyan products are approved to list in China, and the current country has the more granted productions of pharmaceutical producing enterprise.
Mitiglinide Calcium is clinically used for treatment type II diabetes, and mechanism of action is similar to sulfonylureas, but action speed is faster, and half-life period
It is short, the postprandial blood sugar for reducing diabetic is not only improved, and can avoid continuing the hypoglycemia that hypoglycemic causes, with other tradition
Hypoglycemic drug have apparent advantage, can be used as the Remedies for diabetes of a line, therefore the intermediate important as its
S- benzyl succinic acid, have a vast market foreground.
Succinic acid is the reaction mass of S- benzyl succinic acid, therefore should formulate detection of the succinic acid in S- benzyl succinic acid
Method controls the quality of S- benzyl succinic acid.But through retrieving, there is no at present about succinic acid in S- benzyl succinic acid
Detection method of content report.
Succinic acid, English name: Succinic acid, molecular formula: C4H6O4；Molecular weight: 118.09, the following institute of structural formula
Show:
Summary of the invention
In view of the above-mentioned problems, the present invention provides a kind of detection method of impurity succinic acid in S- benzyl succinic acid for the first time.
This method is qualitative, quantitative to succinic acid progress in S- benzyl succinic acid using HPLC detection method, and has carried out methodology validation.
The experiment proved that this method has many advantages, such as that specificity is strong, quick, sensitive, accurate, it can be reliably in S- benzyl succinic acid
Impurity --- succinic acid carries out qualitative and quantitative analysis.
The technical scheme is that in a kind of S- benzyl succinic acid impurity succinic acid detection method, characterized in that will
S- benzyl succinic acid sample dissolution after direct injected using HPLC detection method in S- benzyl succinic acid succinic acid carry out it is qualitative,
It is quantitative.
Wherein, chromatographic condition are as follows:
Chromatographic column: Agilent SB-CN (4.6 × 250mm, 5 μm) or Kromasil 60-5CN (4.6 × 150mm, 5 μ
M), flow velocity: 0.8~1.2ml/min, column temperature: 33~37 DEG C, sample volume: 40 μ l, Detection wavelength: 212nm, mobile phase A: methanol-
Water (containing 0.1% phosphoric acid)=3:97~7:93, Mobile phase B: methanol-water (containing 0.1% phosphoric acid)=43:57~47:53, solvent:
Methanol-water=3:97~7:93.
It is preferred that chromatographic condition is as follows: chromatographic column: Agilent SB-CN (4.6 × 250mm, 5 μm), flow velocity: 1.0ml/min,
Column temperature: 35 DEG C, sample volume: 40 μ l, Detection wavelength: 212nm, mobile phase A: methanol-water (containing 0.1% phosphoric acid)=5:95, flowing
Phase B: methanol-water (containing 0.1% phosphoric acid)=45:55, solvent: methanol-water=5:95.
Condition of gradient elution is shown in Table 1.
1 gradient elution table of table
Further, the present invention initially sets up the standard curve of reference substance (succinic acid), using external standard method S- benzyl
Impurity succinic acid in succinic acid.
Further, under testing conditions of the invention, succinic acid peak RT is 3.8 ± 0.1min；S- benzyl succinic acid peak
RT is 15.48 ± 0.1min, and the standard curve of foundation is y=1317586.7x-0.5, r=0.9999.
As preferred embodiment of the invention, detection method is specific as follows:
1) S- benzyl succinic acid is taken, with solvent dissolution and constant volume, is configured in 1ml containing the molten of 3mg S- benzyl succinic acid
Liquid, as test solution；
2) succinic acid is taken, with solvent dissolution and constant volume, the solution that 1ml contains 0.003mg succinic acid is configured to, as control
Product solution；
3) reference substance solution for test solution and step 2) preparation for taking step 1) to prepare carries out HPLC detection, records color
Spectrogram；
3) according to external standard method, with impurity succinic acid content in calculated by peak area S- benzyl succinic acid.
Advantage of the invention is:
1, succinic acid is the reaction mass of S- benzyl succinic acid.The present invention establishes impurity fourth in S- benzyl succinic acid for the first time
The detection method of diacid controls convenient for the quality to S- benzyl succinic acid, thus improve using S- benzyl succinic acid as
The drug safety of the active constituent of intermediate.
2, good separating effect
The present invention establishes the HPLC method of succinic acid assay in S- benzyl succinic acid for the first time, and carries out to method
Optimization, enables both ingredients to be separated well.As can be seen from Figure 3: succinic acid peak RT is 3.803min；S- benzyl
Succinic acid peak RT is 15.483min, the two good separating effect.
3, the specificity of method is strong, quick, sensitive, accurate
The experiment proved that this method has, specificity strong (specifically for succinic acid), quick, sensitive (detection is limited to
9.3ng is quantitatively limited to 37.4ng), accurate (97.3-101.6%) the advantages that, can be reliably to fourth two in S- benzyl succinic acid
The content of acid carries out qualitative and quantitative analysis.
Detailed description of the invention
Fig. 1 is succinic acid solution chromatogram, and succinic acid peak RT is 3.805；
Fig. 2 is test solution chromatogram, and S- benzyl succinic acid peak RT is 15.482min；
Fig. 3 is mixed solution chromatogram；Succinic acid peak RT is 3.803min；S- benzyl succinic acid peak RT is 15.483min；
Fig. 4 succinic acid linearity curve.
Specific embodiment
Embodiment 1
1 instrument and material
1.1 instruments: 2489 high performance liquid chromatograph of Waters Arc (U.S.'s Waters)；
1.2 reagents: methanol (chromatographic grade), phosphoric acid (chromatographic grade), water is ultrapure water.
2 methods and result
2.1 chromatographic condition
Chromatographic column: Agilent ZORBAX SB-CN chromatographic column (4.6 × 250mm, 5 μm), column temperature: 35 DEG C, sample volume: 40
μl；
Mobile phase A: methanol-water (containing 0.1% phosphoric acid)=5:95, Mobile phase B: methanol-water (containing 0.1% phosphoric acid)=45:
55, solvent: methanol-water=5:95；Flow velocity: 1.0ml/min, condition of gradient elution are shown in Table 1.
Detector: 2489 UV detector of Waters, Detection wavelength: 212nm.
The preparation of 2.2 solution
The preparation of 2.21 specificity solution
Precision weighs succinic acid reference substance 31.15mg, sets 100ml measuring bottle, and solubilizer dissolves and is diluted to scale, obtains solution
A, i.e. reference substance solution.
Precision weighs diethyl succinate 30.21mg, sets 100ml measuring bottle, is diluted to scale with solvent, precision measures
1.0ml sets 10ml measuring bottle, is diluted to scale with solvent, obtains solution b.
Precision weighs benzaldehyde 30.65mg, sets 100ml measuring bottle, is diluted to scale with solvent, precision measures 1.0ml and sets
10ml measuring bottle is diluted to scale with solvent, obtains solution c.
Benzoic acid 3.10mg is weighed, 100ml measuring bottle is set, solubilizer dissolves and be diluted to scale, obtains solution d.
Benzylidene succinic acid 3.06mg is weighed, 100ml measuring bottle is set, solubilizer ultrasonic dissolution is simultaneously diluted to scale, obtains solution
e。
Precision weighs α-phenylethylamine 30.36mg, sets 100ml measuring bottle, is diluted to scale with solvent, precision measures 1.0ml and sets
10ml measuring bottle is diluted to scale with solvent, obtains solution f.
(S)-benzyl succinic acid (R)-α-phenylethylamine salt 3.12mg is weighed, 100ml measuring bottle is set, solubilizer is dissolved and is diluted to
Scale obtains solution g.
Precision measures solution a 1.0ml and sets in 100ml measuring bottle, is diluted to scale with solvent, and obtaining concentration is 0.003115mg/
The reference substance solution of ml.
Precision weighs this product S- benzyl succinic acid 300.23mg, sets 100ml measuring bottle, and solubilizer ultrasonic dissolution is simultaneously diluted to quarter
Degree, obtains test solution.
Precision weighs this product S- benzyl succinic acid 30.26mg, sets 10ml measuring bottle, measures solution a 0.1ml, solution b respectively
Each 1.0ml of~solution f sets above-mentioned same 10ml measuring bottle, and solubilizer dissolves and is diluted to scale, obtains mixed solution.
The preparation of 2.22 linear solvents
Precision weighs succinic acid reference substance 31.15mg, sets in 100ml measuring bottle, and scale is dissolved and be diluted to solvent, accurate
It measures 1.0ml to set in 100ml measuring bottle, is diluted to scale with solvent, obtain the reference substance solution that concentration is 0.003115mg/ml.Point
Do not take 15 μ l of reference substance solution, 20 μ l, 30 μ l, 40 μ l, 50 μ l sample introductions, obtain concentration be respectively 0.001168mg/ml,
The linear solvent of 0.001558mg/ml, 0.002336mg/ml, 0.003115mg/ml, 0.003894mg/ml.
The preparation of 2.23 mark-on test solutions
Totally six parts of weighed S- benzyl succinic acid sample 300mg (being accurate to 0.01mg), sets in 100ml measuring bottle respectively, adds
Enter solution a1.0ml, then solubilizer dissolves and be diluted to scale, as mark-on test solution, the repeatability of calculation method.No
Same date is measured same sample, the Intermediate precision of calculation method.
Prepare the mark-on test solution for adding various concentration succinic acid reference substance solution, the accuracy of calculation method.
2.24 detection
Precision measures 2.21 reference substance solution, test solution and each 40 μ l of mixed solution directly (difference) sample introduction, presses
HPLC detection is carried out according to chromatographic condition shown in 2.1, records chromatogram.The chromatogram difference of three kinds of solution is as shown in Figs. 1-3.From
Fig. 3 is it can be seen that succinic acid peak RT is 3.803min；S- benzyl succinic acid peak RT is 15.483min, and the chromatographic peak of the two can
It is separated well, and other impurities do not interfere the measurement of succinic acid.Linear solvent is detected, then with succinic acid
Concentration is abscissa, and peak area is ordinate, linear standard curve is drawn, by external standard method with calculated by peak area S- benzyl amber
The content of impurity succinic acid in acid.
The verifying of 3 analysis methods
3.1 specificity
The six progress HPLC detections of reference substance solution continuous sample introduction are taken, obtained succinic acid peak area RSD=0.93% is protected
Stay time RSD=0.01%.
Succinic acid and adjacent peak separating degree are 4.88 in mixed solution, and other impurities and sample do not interfere the survey of succinic acid
Fixed, specificity meets the requirements.
3.2 linear relationship
Linear solvent is taken to carry out HPLC measurement, for acquired results using the concentration of succinic acid as abscissa, peak area is ordinate,
Draw standard working curve.Linear equation is y=1317586.7x-0.5, r=0.9999 (see Fig. 4).
3.3 repeatability and Intermediate precision
It takes six parts of solution of same S- benzyl succinic acid sample configuration to carry out repeated experiment, the results are shown in Table 2.
The repeated result of table 2
Six parts of solution of the same S- benzyl succinic acid sample configuration that same date does not take repetition to test carry out repeated experiment,
It the results are shown in Table 3.
3 Intermediate precision result of table
3.4 accuracy
Prepare the test solution that sample adds various concentration succinic acid reference substance solution, the accuracy of calculation method.As a result
It is shown in Table 4.
4 accuracy result of table
3.5 durabilities: taking reference substance solution under specificity item, in 32 hours, repeats sample introduction, reference substance peak area is opposite
Standard deviation is 1.04%；S- benzyl succinic acid sample solution under specificity item is taken, in 8 hours, repeats sample introduction, sample solution
Middle succinic acid is not detected.
3.6 detection limits: the detection limit (LOD) of method is the sample introduction concentration of succinic acid when taking 3 times of signal-to-noise ratio S/N >, is led to
It crosses and is calculated as 9.3ng, percent concentration 0.0075%.
3.7 quantitative limits: the quantitative limit (LOQ) of method is that the sample introduction concentration of succinic acid when taking 10 times of signal-to-noise ratio S/N > is logical
It crosses and is calculated as 37.4ng, percent concentration 0.03%.
4 discuss
The HPLC detection method of succinic acid assay in S- benzyl succinic acid is established herein, and method has been carried out excellent
Change, both ingredients is enable to be separated (such as Fig. 3) well.The experiment proved that this method have specificity it is strong, quickly,
The advantages that sensitive, accurate, reliably the content to succinic acid in S- benzyl succinic acid can carry out qualitative and quantitative analysis.
Succinic acid does not find specific toxicity data without genotoxicity caution structure yet, according to unknown impuritie in ICHQ3A
Requirement, it is≤0.10% that we, which order control limit,.
Embodiment 2: the detection of actual sample
1) S- benzyl succinic acid is taken, with solvent dissolution and constant volume, is configured in 1ml containing the molten of 3mg S- benzyl succinic acid
Liquid, as test solution；
2) succinic acid is taken, with solvent dissolution and constant volume, the solution that 1ml contains 0.003mg succinic acid is configured to, as control
Product solution；
3) take step 1) prepare test solution and step 2) preparation each 40 μ l of reference substance solution directly (difference) into
Sample, using with the identical instrument of embodiment 1 and reagent, carry out HPLC detection according to chromatographic condition shown in 2.1, record color
Spectrogram；
3) according to external standard method, with impurity succinic acid content in calculated by peak area S- benzyl succinic acid.
Three batches of samples of detection with the aforedescribed process are adopted, the results are shown in Table 5:
Succinic acid content in 5 three batches of products of table
| Sample lot number | 1 | 2 | 3 |
| Succinic acid content % | It is not detected | It is not detected | It is not detected |
。
Claims (9)
1. the detection method of impurity succinic acid in a kind of S- benzyl succinic acid, characterized in that dissolve S- benzyl succinic acid sample
Direct injected carries out succinic acid in S- benzyl succinic acid using HPLC detection method qualitative, quantitative afterwards.
2. the detection method of impurity succinic acid in a kind of S- benzyl succinic acid as described in claim 1, characterized in that described
HPLC chromatogram condition are as follows:
Chromatographic column: Agilent SB-CN chromatographic column or Kromasil 60-5 CN chromatographic column；Flow velocity: 0.8~1.2ml/min；Column
Temperature: 33~37 DEG C；Detection wavelength: 212nm；Mobile phase A: methanol-contains water=3:97~7:93 of 0.1% phosphoric acid；Mobile phase B:
Methanol-contains water=43:57~47:53 of 0.1% phosphoric acid.
3. the detection method of impurity succinic acid in a kind of S- benzyl succinic acid as claimed in claim 2, characterized in that the color
Spectral condition is as follows:
Chromatographic column: Agilent SB-CN chromatographic column, 4.6 × 250mm, 5 μm；Flow velocity: 1.0ml/min；Column temperature: 35 DEG C；Sample introduction
Amount: 40 μ l；Detection wavelength: 212nm；Mobile phase A: methanol-contains water=5:95 of 0.1% phosphoric acid, and Mobile phase B: methanol-contains
Water=45:55 of 0.1% phosphoric acid.
4. the detection method of impurity succinic acid in a kind of S- benzyl succinic acid as claimed in claim 3, characterized in that gradient is washed
De- condition, condition of gradient elution are as follows:
5. the detection method of impurity succinic acid in a kind of S- benzyl succinic acid as described in any one of claim 1-4,
It is characterized in, the solvent of S- benzyl succinic acid and succinic acid dissolution use are as follows: methanol-water=5:95 by volume.
6. the detection method of impurity succinic acid in a kind of S- benzyl succinic acid as claimed in claim 5, characterized in that establish fourth
The standard curve of diacid, using the content of impurity succinic acid in external standard method S- benzyl succinic acid.
7. the detection method of impurity succinic acid in a kind of S- benzyl succinic acid as claimed in claim 6, characterized in that described to build
Vertical standard curve is y=1317586.7x-0.5, r=0.9999.
8. the detection method of impurity succinic acid in a kind of S- benzyl succinic acid as claimed in claim 5, characterized in that HPLC inspection
In survey method, succinic acid peak RT is 3.8 ± 0.1min；S- benzyl succinic acid peak RT is 15.48 ± 0.1min.
9. the detection method of impurity succinic acid in a kind of S- benzyl succinic acid as described in any one of claim 6-8,
It is characterized in, the detection method is specific as follows:
1) S- benzyl succinic acid is taken, with solvent dissolution and constant volume, is configured to the solution containing 3mg S- benzyl succinic acid in 1ml,
As test solution；
2) succinic acid is taken, with solvent dissolution and constant volume, is configured to the solution that 1ml contains 0.003mg succinic acid, it is molten as reference substance
Liquid；
3) reference substance solution for test solution and step 2) preparation for taking step 1) to prepare carries out HPLC detection, records chromatography
Figure；
4) according to external standard method, with impurity succinic acid content in calculated by peak area S- benzyl succinic acid.
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| CN110850003A (en) * | 2019-12-16 | 2020-02-28 | 湖南九典制药股份有限公司 | Method for separating impurity succinic acid from ferrous succinate |
| CN112557566A (en) * | 2020-12-12 | 2021-03-26 | 江西济民可信药业有限公司 | Method for detecting corresponding isomer of mitiglinide calcium intermediate S-benzylsuccinic acid |
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Address after: 251400 No. 12, Taixing East Street, Jibei Economic Development Zone, Jiyang District, Jinan City, Shandong Province Patentee after: Shandong Baoyuan Pharmaceutical Co.,Ltd. Address before: Strong in Jiyang County of Ji'nan City, 251400 North Street, Shandong Province Economic Development Zone Patentee before: SHANDONG BOYUAN PHARMACEUTICAL Co.,Ltd. |
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Denomination of invention: A Detection Method of Succinic Acid in S-Benzylsuccinic Acid Effective date of registration: 20221130 Granted publication date: 20210319 Pledgee: Qilu bank Limited by Share Ltd. Ji'nan science and technology innovation center sub branch Pledgor: Shandong Baoyuan Pharmaceutical Co.,Ltd. Registration number: Y2022980023658 |