CN109575150A - A kind of extraction process of Cordyceps sinensis polysaccharide - Google Patents
A kind of extraction process of Cordyceps sinensis polysaccharide Download PDFInfo
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- CN109575150A CN109575150A CN201811299518.4A CN201811299518A CN109575150A CN 109575150 A CN109575150 A CN 109575150A CN 201811299518 A CN201811299518 A CN 201811299518A CN 109575150 A CN109575150 A CN 109575150A
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- Prior art keywords
- cordyceps sinensis
- extraction process
- sinensis polysaccharide
- polysaccharide
- extraction
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Sustainable Development (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention provides a kind of extraction process of Cordyceps sinensis polysaccharide, the extraction process includes the extraction of polysaccharide and the purification of polysaccharide.The polysaccharide extracting process can high efficiency extraction Cordyceps sinensis polysaccharide, reduce cost, have important economic value and wide market application potential, be suitble to industrialized production.
Description
Technical field
The invention belongs to field of biological product, relate particularly to a kind of extraction process of Cordyceps sinensis polysaccharide.
Background technique
Cordyceps sinensis polysaccharide is a kind of polymer composite, its one of main active as cordyceps sinensis, is content in cordyceps sinensis
Highest pharmacological active substance.A large amount of pharmacological experiment shows Cordyceps sinensis polysaccharide with antitumor, adjusting is immune, hypoglycemic, drop
Blood lipid, antiatherosclerosis, protection kidney, analgesia and the anti-effect of putting.
The method that tradition extracts Cordyceps sinensis polysaccharide in cordyceps sinensis, such as decocting method, circumfluence method, continuous backflow method, infusion process, percolation
Deng, be known can in detail.But these types of method haves the shortcomings that a common production cycle is long；Tradition is extracted in Cordyceps militaris
The method of cordycepin needs a large amount of extraction solvent, extraction effect if water bath reflux method, soxhlet extraction are extracting technology
Rate is low.And have that energy consumption is big, and extract yield is relatively low, production cost is higher and disadvantage.
Summary of the invention
In order to solve drawbacks described above, the object of the present invention is to provide a kind of extraction process of Cordyceps sinensis polysaccharide, the Polyose extractions
Technique can reduce cost with high efficiency extraction Cordyceps sinensis polysaccharide, have important economic value and wide market application potential.
Technical solution to achieve the above object is as follows: a kind of extraction process of Cordyceps sinensis polysaccharide, comprising steps of
1. suction filtration processing is carried out to strain, then will filter obtained mycelium be put into dried in air dry oven it is completely dry
It is dry, weigh its quality；
2. after having claimed quality, mycelium being shredded and is put into mortar, pours into liquid nitrogen and ground, it can be with liquid nitrogen grinding
By the more abundant, thinner of cordyceps mycelia grinding, the amount that extraction obtains Cordyceps sinensis polysaccharide will be higher；
3. pulverized powder will be ground to pour into bottle, pure water is added, and is put into ultrasonic wave and is soaked with the ultrasound of 120W/40KHZ frequency
It mentions；
4. after having extracted, being centrifuged to obtain supernatant with centrifuge, supernatant is stored in test tube.
Wherein, the time that 1. is step is dried 6-7 hour.2. the middle fineness ground is 60 mesh to step.Step 3. in plus
The pure water entered is 15ml, and the time of the ultrasound extraction is 30min.Step 4. in the revolving speed that is centrifuged be 4000rpm, the time is
15min。
Further, 4. step further comprises the steps of: afterwards
5. the purification of polysaccharide: ethyl alcohol is added to supernatant, precipitating filters to obtain thick solid polysaccharide intracellular, is cleaned with ether solid
It is spare to save refrigerator for body, filtering, the thick solid polysaccharide intracellular of be removed fat and protein.The ethyl alcohol of addition is 80% second
Alcohol, ethanol consumption are 2 times of supernatant volumes, are then precipitated for 24 hours.
The beneficial effects of the present invention are: providing a kind of extraction process of Cordyceps sinensis polysaccharide, which can be high
Effect extracts Cordyceps sinensis polysaccharide, reduces cost, has important economic value and wide market application potential, is suitble to industry metaplasia
It produces.
Specific embodiment
Following steps are only to illustrate the technical solution of the disclosure, rather than its limitations；Although referring to These steps
The disclosure is described in detail, but those skilled in the art should understand that: it still can be to aforementioned each step
Technical solution documented by rapid is modified, or equivalent substitution of some or all of the technical features；And these
It modifies or replaces, the range of each step technique scheme of the disclosure that it does not separate the essence of the corresponding technical solution.
Embodiment 1: the culture of cordyceps species
1. the activation of strain
Aweto fungus in refrigerator will be stored in be inoculated on potato culture medium flat plate, be put into 25 DEG C of incubator, trained
It supports three days, completes the activation of strain.
2. expanding culture
The bacterium to grow after picking activation is linked into potato fluid nutrient medium and is put into shaking table, at 25 DEG C, 150r/min
Under conditions of, shaken cultivation ten days.
3. fermented and cultured
Configure the culture medium of following ratio: glucose 10g/L, sucrose 25g/L, corn flour 10g/L, maltose 5g/L, egg
White peptone 4g/L, ammonium nitrate 3g/L, magnesium sulfate 0.8g/L, dipotassium hydrogen phosphate 0.4g/L, potassium dihydrogen phosphate 0.4g/L, ferrous sulfate
1.4g/L, vitamin B1 0.03g/L, glycine 0.8g/L, yeast powder 4g/L and dregs of beans 4g/L after bacterium of having gone out, take expansion to cultivate
Bacterium solution be inoculated into the culture medium of above-mentioned bacterium of having gone out, be put into shaking table, at 25 DEG C, under conditions of 150r/min, shaken cultivation ten
It.
Embodiment 2: Polyose extraction
The good strain of fermented and cultured in embodiment 1 is subjected to suction filtration processing, the mycelium filtered contains intracellular thick more
Sugar, the mycelium filtered are put into 6-7 hour of baking in air dry oven, and mycelium is completely dried at this time, then weighs its matter
It measures and records；After having claimed quality, mycelium is shredded and is put into mortar, poured into liquid nitrogen and ground, mycelium is ground into 60
Mesh fineness or so；Pulverized powder will be ground to pour into wide-mouth bottle, add 15ml pure water, and be put into ultrasound extraction in ultrasonic wave
30min；After having extracted, under conditions of 4000rpm, 15min, it is centrifuged to obtain supernatant with centrifuge, supernatant is stored in test tube
In.
Embodiment 3: the purification of polysaccharide
Supernatant obtained in embodiment 2 is placed in wide-mouth bottle, 80% ethyl alcohol of 2 times of supernatant volumes is added, is precipitated
For 24 hours, thick solid polysaccharide intracellular is filtered to obtain, cleans solid 2-3 times with 20ml ether, is filtered, be removed fat and protein
It is spare to save refrigerator for thick solid polysaccharide intracellular.
Claims (7)
1. a kind of extraction process of Cordyceps sinensis polysaccharide, which is characterized in that comprising steps of
1. carrying out suction filtration processing to strain, it then will filter obtained mycelium and dry；
It is put into mortar 2. mycelium is shredded, pours into liquid nitrogen and ground；
3. pure water is added to pulverized powder is ground, and carry out ultrasonic extraction；
4. after having extracted, being centrifuged to obtain supernatant with centrifuge, supernatant is stored in test tube.
2. a kind of extraction process of Cordyceps sinensis polysaccharide according to claim 1, which is characterized in that 1. time that step is dried
For 6-7 hour.
3. a kind of extraction process of Cordyceps sinensis polysaccharide according to claim 1, which is characterized in that step 2. in grind it is thin
Degree is 55-60 mesh.
4. a kind of extraction process of Cordyceps sinensis polysaccharide according to claim 1, which is characterized in that step 3. in be added it is pure
Water is 15ml, and the time of the ultrasound extraction is 30min.
5. a kind of extraction process of Cordyceps sinensis polysaccharide according to claim 1, which is characterized in that step 4. in be centrifuged turn
Speed is 4000rpm, time 15min.
6. a kind of extraction process of Cordyceps sinensis polysaccharide according to claim 1, which is characterized in that 4. step further includes step afterwards
It is rapid:
5. the purification of polysaccharide: ethyl alcohol is added to supernatant, precipitating filters to obtain thick solid polysaccharide intracellular, cleans solid, mistake with ether
Filter, the thick solid polysaccharide intracellular of be removed fat and protein, freezen protective are spare.
7. a kind of extraction process of Cordyceps sinensis polysaccharide according to claim 6, which is characterized in that the ethyl alcohol of addition is 80%
Ethyl alcohol, ethanol consumption are 2 times of supernatant volumes, are then precipitated for 24 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811299518.4A CN109575150A (en) | 2018-11-01 | 2018-11-01 | A kind of extraction process of Cordyceps sinensis polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811299518.4A CN109575150A (en) | 2018-11-01 | 2018-11-01 | A kind of extraction process of Cordyceps sinensis polysaccharide |
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| CN109575150A true CN109575150A (en) | 2019-04-05 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20220287251A1 (en) * | 2019-08-09 | 2022-09-15 | Carolina Truffiéres Llc | Methods and compositions for the production of ectomycorrhizal mycelia and methods of use thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1560085A (en) * | 2004-03-05 | 2005-01-05 | 君 贺 | Process for extracting cordycepic polyose |
| CN101124988A (en) * | 2007-09-25 | 2008-02-20 | 江苏瑞迪生科技有限公司 | Method for extracting refined cordycepin and cordyceps polysaccharide from Cordyceps militaris |
| CN105732832A (en) * | 2014-12-10 | 2016-07-06 | 黑龙江众生生物工程有限公司 | Preparation method of cordyceps militaris polysaccharide |
-
2018
- 2018-11-01 CN CN201811299518.4A patent/CN109575150A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1560085A (en) * | 2004-03-05 | 2005-01-05 | 君 贺 | Process for extracting cordycepic polyose |
| CN101124988A (en) * | 2007-09-25 | 2008-02-20 | 江苏瑞迪生科技有限公司 | Method for extracting refined cordycepin and cordyceps polysaccharide from Cordyceps militaris |
| CN105732832A (en) * | 2014-12-10 | 2016-07-06 | 黑龙江众生生物工程有限公司 | Preparation method of cordyceps militaris polysaccharide |
Non-Patent Citations (1)
| Title |
|---|
| 聂斌英 等: ""亚香棒虫草内生真菌C-10转化多糖发酵工艺探讨"", 《中国果蔬》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20220287251A1 (en) * | 2019-08-09 | 2022-09-15 | Carolina Truffiéres Llc | Methods and compositions for the production of ectomycorrhizal mycelia and methods of use thereof |
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Application publication date: 20190405 |