CN109568170A - A kind of suncream and preparation method thereof - Google Patents
A kind of suncream and preparation method thereof Download PDFInfo
- Publication number
- CN109568170A CN109568170A CN201910077364.2A CN201910077364A CN109568170A CN 109568170 A CN109568170 A CN 109568170A CN 201910077364 A CN201910077364 A CN 201910077364A CN 109568170 A CN109568170 A CN 109568170A
- Authority
- CN
- China
- Prior art keywords
- protein
- suncream
- preparation
- parts
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/70—Biological properties of the composition as a whole
- A61K2800/72—Hypo-allergenic
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
Abstract
A kind of suncream and preparation method thereof, the present invention relates to cosmetic technical field, a kind of it is an object of the present invention to provide absorbing abilities protein suncream that strong, empty calory generates.UV Absorption albumen is a kind of epicyte protein, very stable can be gathered in liposome after isolating and purifying out, and the suncream that becoming one kind for it can produce and use provides primary condition.Most importantly, UV Absorption albumen can be converted to the energy of absorption the variation of protein tridimensional conformation, therefore the generation of thermal energy is not present, and because the molecular weight of single protein molecule is more much bigger than the molecular weight of single chemical sunscreen molecule, so the luminous energy of single protein conversion is more much more than chemical molecular, therefore protein is sun-proof has stronger absorbing ability than chemical sunscreen.
Description
Technical field
The present invention relates to cosmetic technical field, more particularly, to sun-proof protein suncream.
Background technique
Sunscreen product principle on current market is related to physical sunscreen and chemical sunscreen, and wherein the suncream of physical sunscreen is former
Sub- particle is sheet, and when spreadable on the face, sunlight is reflected as mirror, sun-proof to achieve the purpose that.But physics is anti-
The limitation for shining product is more, and for people such as deeper to the colour of skin, polishing effect may be less natural；Quality is more dry, to skin
It owes to moisten for the more dry people of matter；Be not suitable for doing whole body sun-proof；And makeup removal products help removal must be used clean etc..
Chemical sunscreen frost is come sun-proof using the principle for absorbing ultraviolet light, is often transmission substance, absorbable ultraviolet light makes its turn
It turns to molecular vibrational energy or thermal energy reaches sun-proof effect, but the sun-proof dynamics of chemical sunscreen product at present on the market is low, needs
It is continuously replenished and sun-proof reaches ideal effect.And general chemistry is sun-proof larger to skin burden, be easy to cause skin allergy.
Summary of the invention
The protein suncream that it is an object of the present invention to provide a kind of absorbing abilities by force, empty calory generates.UV Absorption
Albumen is a kind of epicyte protein, very stable can be gathered in liposome after isolating and purifying out, and becoming one kind for it can
Primary condition is provided with the suncream of production and application.Most of all, UV Absorption albumen can convert the energy of absorption
At the variation of protein tridimensional conformation, therefore the generation of thermal energy is not present, and because of the molecular weight ratio of single protein molecule
The molecular weight of single chemical sunscreen molecule is much bigger, so the luminous energy of single protein conversion is more much more than chemical molecular, because
This protein is sun-proof to have stronger absorbing ability than chemical sunscreen.
A kind of epicyte protein can be applied in suncream by present invention proposition.The absorbing ability of the epicyte protein compares mesh
Preceding known extinction albumen is 50 to 100 times high, can largely absorb UVA and UVB, the sun-proof result of significant increase suncream.
After this cell membrane protein absorbs ultraviolet light, energy can be converted to the change for changing the molecular link of its three-dimensional conformation
Change.The primary raw material of this product is produced in a manner of biofermentation.The zymotechnique of this product uses special Escherichia coli first
Biofermentation is carried out for raw material, the volume capacity size of fermentor, which can according to need, carries out different selections.
Technical solution provided by the invention is as follows:
A kind of suncream, the suncream include UV Absorption protein, what the protein can cause will to absorb
Energy is converted to the variation of the molecular link of protein tridimensional conformation.
A kind of preparation method of suncream, the preparation method include the following steps:
First: preparing the raw material of following parts by weight: 5 ~ 7 parts of yeast powder;10 ~ 15 parts of tryptone；5 ~ 9 parts of sodium chloride；
Second: mixing above-mentioned raw materials with deionized water 800-1200 parts by weight, sterilizing after deploying uniformly；
Third: injecting fermentor, and the Escherichia coli that implantation quality volume ratio is 1 gram: 5000 milliliter are former after being cooled to room temperature, into
Row fermentation, fermentation time are 20-30 hour, and temperature is 35-37 degrees Celsius, pH value 7.2-7.5；
4th: being concentrated after the thallus after being fermented, first centrifugation obtains thallus after concentration, then with buffer is resuspended to obtain dense
Contracting thallus liquid；Concentration ratio is 1:1；The buffer is by double 3 ~ 7 parts by weight of triaminopropane and 1 ~ 3 parts by weight of sodium chloride
With, wherein pH=7.5；
5th: carrying out the broken of thallus with fluid breakup instrument, the aperture of fluid breakup instrument is 0.2 micron；
6th: the UV Absorption albumen being purified into bacterial cell disruption mixed liquor, purification process protein affinity chromatography method
Purifying；
7th: being washed with buffer, the ultraviolet light protein purified；The buffer is by double 3 ~ 7 weights of triaminopropane
It measures part and 1 ~ 3 parts by weight of sodium chloride is matched, wherein pH=7.5；
8th: 3 ~ 5 parts by weight of moisturizer hyaluronic acid and 5 ~ 7 parts by weight of thickener gelatin, ultraviolet photoprotein after purification is added
10 ~ 15 parts by weight of matter, blender stir evenly, and obtain suncream.
The present invention, as suncream main component, improves the anti-power of chemical sunscreen with the extremely strong epicyte protein of extinction power
Spend low defect.
Suncream provided by the invention solves the disadvantage that chemical sunscreen easily causes allergy, and protein is the daily height of human body
Frequency contact material, a example that can generate allergy to protein seldom occur.Main component of the invention is protein, is free of metal
Etc. the substance for easily causing allergy, thus bear for skin smaller.
The participation of sun-proof not metallics provided by the invention, because allowing people not feel good without generating heat, to chase after
It asks the user of high Experience Degree to provide new, better choice, expands sunscreen product type in the market.
Specific embodiment
Zymotechnique of the invention uses special Escherichia coli to carry out biofermentation, the body of fermentor for raw material first
Product amount of capacity, which can according to need, carries out different selections.Formula below is as unit of each material weight part: 1) ferment
6 parts of female powder;2) 13 parts of tryptone；3) 7 parts of sodium chloride；4) it is needed with 1000 parts of deionized water mixing above-mentioned material allotments
After volume, the sterilizing of 120 celsius temperatures；5) the large-scale fermentor to sterilize, and implantation quality body are injected after being cooled to room temperature
The Escherichia coli raw material that product is 1:5000 than (gram: milliliter), ferment, and fermentation time is 25 hours, and temperature is 37 to take the photograph
Family name's degree, pH value are about 7.5；6) concentration of 1:1 is carried out after a large amount of thallus after being fermented, first centrifugation obtains thallus, then uses
3 ~ 7 parts of double triaminopropanes, 1 ~ 3 part of sodium chloride, buffer is matched to obtain in pH=7.5, is resuspended to obtain concentration thallus with this buffer
Liquid；7) the broken of thallus is carried out with the fluid breakup instrument of 0.2 micron pore size；8) pass through the side of common protein affinity chromatography
Method, the UV Absorption albumen being purified into bacterial cell disruption mixed liquor；9) pass through double 3 ~ 7 parts of triaminopropanes, sodium chloride 1 ~ 3
Part, pH=7.5, the washing of this buffer, the ultraviolet light protein purified；10) 4 parts of general moisturizer and thickener 6 is added
Part, 12 parts of protein after purification, blender stirs evenly, and obtains suncream.
By the protein in above-mentioned suncream by mass spectrometric analysis, not any metal component as the result is shown；And
This protein is subjected to full spectrum light and absorbs test, as the result is shown in the region (320nm) UV UV A and the area UV UV B (280nm)
There is a very strong absorbance value in domain, absorbing ability value, molar absorption coefficient, and UVA is up to 2,000,000 every mole centimetre of (M-1cm-1),
UVB is 3,700,000 every mole centimetre of (M-1cm-1), it is more every than the absorptivity 6.6 ten thousand of traditional chemical sunscreen substance aminobenzoic acid
Mole centimetre (M-1cm-1) nearly 30 to 50 times high.
Claims (9)
1. a kind of suncream, which is characterized in that the suncream includes UV Absorption protein.
2. a kind of suncream as described in claim 1, which is characterized in that the protein can cause the energy that will be absorbed to turn
It is melted into the variation of the molecular link of protein tridimensional conformation.
3. a kind of preparation method of suncream, which is characterized in that the preparation method includes the following steps:
First: preparing the raw material of following parts by weight: 5 ~ 7 parts of yeast powder;10 ~ 15 parts of tryptone；5 ~ 9 parts of sodium chloride；
Second: mixing above-mentioned raw materials with deionized water, sterilizing after deploying uniformly；
Third: injecting fermentor, and the Escherichia coli that implantation quality volume ratio is 1 gram: 5000 milliliter are former after being cooled to room temperature, into
Row fermentation, fermentation time are 20-30 hour, and temperature is 35-37 degrees Celsius, pH value 7.2-7.5；
4th: being concentrated after the thallus after being fermented, first centrifugation obtains thallus after concentration, then with buffer is resuspended to obtain dense
Contracting thallus liquid；
5th: carrying out the broken of thallus with fluid breakup instrument；
6th: the UV Absorption albumen being purified into bacterial cell disruption mixed liquor；
7th: being washed with buffer, the ultraviolet light protein purified；
8th: 3 ~ 5 parts by weight of moisturizer and 5 ~ 7 parts by weight of thickener, 10 ~ 15 weight of ultraviolet light protein after purification is added
Part, blender stirs evenly, and obtains suncream.
4. preparation method as claimed in claim 3, which is characterized in that the buffer is by double 3 ~ 7 weight of triaminopropane
Part and 1 ~ 3 parts by weight of sodium chloride are matched, wherein pH=7.5.
5. preparation method as claimed in claim 3, which is characterized in that concentration ratio is 1:1 in four steps.
6. preparation method as claimed in claim 3, which is characterized in that the aperture of fluid breakup instrument is 0.2 micro- in the 5th step
Rice.
7. preparation method as claimed in claim 3, which is characterized in that pure with protein affinity chromatography method in the 6th step
Change.
8. preparation method as claimed in claim 3, which is characterized in that the moisturizer is hyaluronic acid.
9. preparation method as claimed in claim 3, which is characterized in that the thickener is gelatin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910077364.2A CN109568170A (en) | 2019-01-28 | 2019-01-28 | A kind of suncream and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910077364.2A CN109568170A (en) | 2019-01-28 | 2019-01-28 | A kind of suncream and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109568170A true CN109568170A (en) | 2019-04-05 |
Family
ID=65918158
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910077364.2A Pending CN109568170A (en) | 2019-01-28 | 2019-01-28 | A kind of suncream and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109568170A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102302417A (en) * | 2011-09-13 | 2012-01-04 | 佛山市安安美容保健品有限公司 | Comprehensive-effect sunscreen lotion containing oceanic biological active substances |
| CN104146935A (en) * | 2013-05-15 | 2014-11-19 | 范洁琼 | Whitening and sunscreen cream and preparation method thereof |
| CN105213269A (en) * | 2015-10-29 | 2016-01-06 | 桂林华诺威基因药业有限公司 | A kind of sunscreen cream |
| CN105456146A (en) * | 2014-08-07 | 2016-04-06 | 青岛蓝农谷农产品研究开发有限公司 | High-strength anti-ultraviolet-ray silk fibroin sunscreen cream |
-
2019
- 2019-01-28 CN CN201910077364.2A patent/CN109568170A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102302417A (en) * | 2011-09-13 | 2012-01-04 | 佛山市安安美容保健品有限公司 | Comprehensive-effect sunscreen lotion containing oceanic biological active substances |
| CN104146935A (en) * | 2013-05-15 | 2014-11-19 | 范洁琼 | Whitening and sunscreen cream and preparation method thereof |
| CN105456146A (en) * | 2014-08-07 | 2016-04-06 | 青岛蓝农谷农产品研究开发有限公司 | High-strength anti-ultraviolet-ray silk fibroin sunscreen cream |
| CN105213269A (en) * | 2015-10-29 | 2016-01-06 | 桂林华诺威基因药业有限公司 | A kind of sunscreen cream |
Non-Patent Citations (2)
| Title |
|---|
| 45YUFD: "《1-大肠杆菌重组蛋白表达提取及纯化实验》", 12 October 2014, HTTP://WWW.DOC88.COM/P-3817937835167.HTML，道客巴巴 * |
| 李平，等: "《部队消毒工作手册》", 31 December 2012, 人民军医出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102387996B (en) | Electrolyzer for activating products and media and devices comprising such an electrolyzer | |
| CN105232442A (en) | Plant antioxidation amino acid cleanser and preparation method thereof | |
| CN110123661B (en) | Moisturizing, anti-inflammatory and anti-wrinkle composition containing ceramide, and preparation method and application thereof | |
| CN108852957A (en) | A kind of moisturizing, whitening, anti-inflammatory, anti-acne ferment facial mask and preparation method thereof | |
| CN103565729A (en) | Lycium ruthenicum mill moisturizing and repairing aqua gel and preparation method of aqua gel | |
| CN101491489A (en) | Camellia skin-protection essential oil | |
| CN109464377A (en) | A kind of antibacterial biological bath liquor and preparation method | |
| CN111632020A (en) | Anti-aging and moisturizing cosmetic containing stem cell compound factors | |
| JP4945556B2 (en) | Cell application skin external preparation composition | |
| CN107432849A (en) | A kind of micromolecule hyaluronic acid skin base solution and preparation method thereof | |
| CN109431860A (en) | Cosmetics-stage poly (sodium aspartate) and daily chemical product containing the ingredient | |
| CN109820751A (en) | A kind of suncream and preparation method thereof | |
| CN109528562A (en) | A kind of ozone wire drawing cleansing mousse of use in personal care and preparation method thereof | |
| CN105769670A (en) | Skin anti-aging essence and preparation method and application thereof | |
| CN109568170A (en) | A kind of suncream and preparation method thereof | |
| CN109568236A (en) | A kind of sponge micropin reconciles Essence and preparation method thereof | |
| CN113018236A (en) | Cosmetic with scar removing effect and preparation method and preparation device thereof | |
| CN101637437B (en) | Sericin latex with functions of whitening skin and screening sun and preparation technology thereof | |
| CN116531485A (en) | Preparation method of human anti-aging cell substance | |
| CN107213029A (en) | A kind of moisturizing toner for adding low-temperature superoxide dismutase and preparation method thereof | |
| CN112076144A (en) | Soft skin and face cleaning powder and preparation method thereof | |
| CN105832638A (en) | Whitening pearl facial mask moist essence with deep moisturizing function and preparation method thereof | |
| CN112022792A (en) | Preparation method of edible traditional Chinese medicine hair dye with homology of medicine and food | |
| CN111714437A (en) | Beautifying liquid for removing freckles and wrinkles and preparation method thereof | |
| CN111671678A (en) | Shampoo and bath foam and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: 511500 Room 708, West Junction of Yongan Road and Fengcheng Road, Xincheng West No. 2 District, Qingyuan City, Guangdong Province (Office only) Applicant after: QINGYUAN JIEANKAI BIOLOGICAL TECHNOLOGY Co.,Ltd. Address before: 511500 Room 708, West Junction of Yongan Road and Fengcheng Road, Xincheng West No. 2 District, Qingyuan City, Guangdong Province (Office only) Applicant before: QINGYUAN JIEANKAI BIOLOGICAL TECHNOLOGY Co.,Ltd. |
|
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190405 |