CN109566919A - Microorganism formulation, aquatic feeds and aquaculture method - Google Patents
Microorganism formulation, aquatic feeds and aquaculture method Download PDFInfo
- Publication number
- CN109566919A CN109566919A CN201910108472.1A CN201910108472A CN109566919A CN 109566919 A CN109566919 A CN 109566919A CN 201910108472 A CN201910108472 A CN 201910108472A CN 109566919 A CN109566919 A CN 109566919A
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- microorganism formulation
- probiotics
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- lactobacillus
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
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- A—HUMAN NECESSITIES
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- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
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- A23K—FODDER
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- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
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- A23V2400/125—Casei
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
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-
- A—HUMAN NECESSITIES
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- A23V2400/21—Streptococcus, lactococcus
- A23V2400/225—Faecalis
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Abstract
The invention discloses a kind of microorganism formulation, aquatic feeds and aquaculture methods, are related to technical field of aquaculture.The microorganism formulation includes bacteriophagic Bdellovibrio and probiotics；Preferably, any one of the probiotics in German-style lactobacillus subspecies bulgaricus, Lactobacillus delbrueckii subsp. lactis, enterococcus faecalis, Lactobacillus casei, lactobacillus acidophilus, Rhodopseudomonas palustris, clostridium butyricum, bacillus laterosporus, bacillus pumilus, bacillus coagulans, bacillus licheniformis, bacillus subtilis and lactobacillus plantarum.The microorganism formulation has the food ration of enhancing aquiculture animal, the features such as improving its growth rate, survival rate and immunity of organisms, in addition, the microorganism formulation can also be effectively prevented and treated because of pathogenic bacteria such as vibrio parahaemolytious, Vibrio harveyi, luminous Vibrio, vibrio alginolyticus, Vibrio campbellii, Vibrio flurialis, Vibrio vulnificus, Aeromonas, comma bacillus, pseudomonad, salmonellas caused by disease problems.
Description
Technical field
The present invention relates to technical field of aquaculture, support in particular to microorganism formulation, aquatic feeds and aquatic products
Grow method.
Background technique
The feed addictive of drug containing ingredient using more and more extensive, especially in recent years antibiotic in aquatic feeds
In application, antibiotic plays a significant role in terms of the prevention and treatment of Animal diseases, but also gives cultivated animals simultaneously
And the mankind bring certain side effect.For antibiotic first while killing pathogenic bacteria, also by animal, intracorporal other are beneficial
Bacterium is killed simultaneously, seriously destroys original profitable strain in animal body, and it is flat to have broken the microorganism having by oneself in body
Weighing apparatus, the microbial balance system being constantly broken cause very big influence to immunity of organisms, cause cultivated animals increasingly
It is easy morbidity；Secondly, while feeding antibiotic removes pathogenic bacteria for a long time, it is easy to lead to drug resistance problems, finally
Cause to cultivate success rate and antibiotic residue is increasingly severe.
Therefore finding the substitute with substitute antibiotics is generally accepted in current industry and all in the direction of effort.
In consideration of it, the present invention is specifically proposed.
Summary of the invention
The purpose of the present invention is to provide a kind of microorganism formulation, which can be used for raising into aquaculture
Aquiculture animal such as fish, Penaeus Vannmei, Macrobrachium rosenbergii, cray and crab, sea cucumber, shellfish etc. are fed, can effectively be killed
It goes out because of the internal pathogenic bacteria such as vibrios, Aeromonas, pseudomonad, salmonella of cultivated animals etc. of pathogenic infection, has
Enhance the food ration of cultivated animals, the features such as improving its growth rate, survival rate and immunity of organisms, in addition, the microorganism formulation
It can also be effectively prevented and treated because of vibrio parahaemolytious, Vibrio harveyi, luminous Vibrio, vibrio alginolyticus, Vibrio campbellii, river arc
Disease problems caused by the pathogenic bacteria such as bacterium, Vibrio vulnificus, Aeromonas, comma bacillus, pseudomonad, salmonella.
Another object of the present invention is to provide the preparation methods of mentioned microorganism preparation.The preparation method is simply easily grasped
Make, prepared microorganism formulation can be used for feeding aquiculture animal such as fish, shrimp and crab, sea in aquaculture
Ginseng, shellfish etc., to kill the internal pathogenic bacteria of the cultivated animals because of pathogenic infection.
Another object of the present invention is to provide a kind of aquatic feeds, feed aquiculture animal example using the aquatic feeds
Such as fish, shrimp and crab, sea cucumber, shellfish, can treat or prevent pathogenic infection, enhance the food ration of cultivated animals, improve
Its growth rate, survival rate and immunity of organisms etc., at the same prevent and treat because vibrio parahaemolytious, Vibrio harveyi, luminous Vibrio,
The pathogenic bacteria such as vibrio alginolyticus, Vibrio campbellii, Vibrio flurialis, Vibrio vulnificus, Aeromonas, comma bacillus, pseudomonad, salmonella
Caused disease.
A further object of the present invention is to provide a kind of aquaculture methods, for example using this method Aquatic farming animals
Fish, shrimp and crab, sea cucumber, shellfish etc. can effectively treat and prevent pathogenic infection, enhance the food ration of cultivated animals, mention
Its high growth rate, survival rate and immunity of organisms etc., while preventing and treating because of vibrio parahaemolytious, Vibrio harveyi, fluorescence arc
Bacterium, vibrio alginolyticus, Vibrio campbellii, Vibrio flurialis, Vibrio vulnificus, Aeromonas, comma bacillus, pseudomonad, salmonella etc. cause
Germ disease.
The present invention is implemented as follows:
On the one hand, the present invention provides a kind of microorganism formulations comprising bacteriophagic Bdellovibrio and probiotics；
Preferably, the probiotics is selected from German-style lactobacillus subspecies bulgaricus, Lactobacillus delbrueckii subsp. lactis, excrement intestines ball
Bacterium, lactobacillus acidophilus, Rhodopseudomonas palustris, clostridium butyricum, bacillus laterosporus, bacillus pumilus, coagulates at Lactobacillus casei
Tie any one in bacillus, bacillus licheniformis, bacillus subtilis and lactobacillus plantarum.
Bacteriophagic Bdellovibrio is separated from Kidney bean leaf blight aeruginosa atcc 11355 by Stolp and Petzhold et al.
It was found that one kind to attack and crack other bacteriums complete the bacterial parasite of own growth and breeding.Bacteriophagic Bdellovibrio has similar
The effect of bacteriophage, to causing the pathogenic bacteria of aquaculture creature disease to have effects that good cracking, removing, while phagocytosis
Bdellovibrio does not have any side effect.It has the unique history of life, first is that freely moving about outside host cell, second be
Breeding, growth in host cell.In aquaculture process, to such as vibrio parahaemolytious, vibrio alginolyticus, luminous Vibrio, molten algae
The diseases bacterium such as vibrios, Vibrio campbellii, Vibrio flurialis, Vibrio vulnificus, Aeromonas, comma bacillus, pseudomonad, salmonella has
Effect is killed well.
The form application of liquid or the single bacterial strain of freeze-dried powder, drawback have been to act on phage bdellovibro preparation at present
Single effect, and not enough persistently, have the characteristics that rebound after use in a period of time, while the bacteriophagic Bdellovibrio of liquid is with new
The increase of old metabolism, poisonous and harmful substance is cumulative, causes the effective component of product fewer and fewer, effect is caused for example to grow
Rate, survival rate and immunity of organisms etc. are had a greatly reduced quality.
The present invention is creatively by bacteriophagic Bdellovibrio and probiotics such as German-style lactobacillus subspecies bulgaricus, De Shi cream bar
Bacterium lactic acid subspecies, enterococcus faecalis, Lactobacillus casei, lactobacillus acidophilus or lactobacillus plantarum are compounded, by optimizing each strain
Between viable count ratio, formed complex microorganism preparations, be used in aquaculture, treatment or prevention vibrio infection can be played
Effect, while enhancing the food ration of cultivated animals, its growth rate, survival rate and immunity of organisms improved, in addition, the microorganism
Preparation can also be effectively prevented and treated because of vibrio parahaemolytious, Vibrio harveyi, luminous Vibrio, vibrio alginolyticus, Vibrio campbellii, river
Disease problems caused by the pathogenic bacteria such as vibrios, Vibrio vulnificus, Aeromonas, comma bacillus, pseudomonad, salmonella.
In above-mentioned probiotics, German-style lactobacillus subspecies bulgaricus, Lactobacillus delbrueckii subsp. lactis, cheese cream bar
Bacterium, enterococcus faecalis, lactobacillus acidophilus and lactobacillus plantarum belong to one kind of lactic acid bacteria class, these bacterium can utilize carbon aquation
The bacterium that a large amount of lactic acid are generated after object is fermented is closed, lactic acid bacteria is deposited extensively in aquiculture animal and human digestive system
It is being that body is essential and very important flora.These bacterium have stronger acid producing ability, and it is dynamic can effectively to control cultivation
Acid-base property in object body, enteron aisle, the lactic acid generated have good inhibiting effect for pathogenic bacteria, are mainly manifested in inhibition
Vibrio parahaemolytious, vibrio alginolyticus, Vibrio harveyi, luminous Vibrio, vibrio alginolyticus, Vibrio campbellii, Vibrio flurialis, Vibrio vulnificus, gas
The breeding and growth of monad, comma bacillus, pseudomonad, salmonella etc., while these lactic acid bacterias disappear to cultivated animals
Change process plays good facilitation, improves the digestive utilization ratio of feed, promotes food calling etc..
Further, in some schemes of the invention, the viable count of the bacteriophagic Bdellovibrio and the probiotics
Than for (1-104):(1-106)。
Further, in some schemes of the invention, the viable count of the bacteriophagic Bdellovibrio and the probiotics
Than for 1:(1-106)。
Further, in some schemes of the invention, the viable count of the bacteriophagic Bdellovibrio and the probiotics
Than for 1:1,1:10,1:100,1:1000,1:10000,1:100000 or 1:1000000.
Further, in some schemes of the invention, the viable count of the bacteriophagic Bdellovibrio and the probiotics
Than for (1-104):1。
Further, in some schemes of the invention, the viable count of the bacteriophagic Bdellovibrio and the probiotics
Than for 10:1,100:1,1000:1 or 10000:1.
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 104-1×109pfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the probiotics contains
Amount is 1 × 105-1×1010cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 104pfu、1×105pfu、1×106pfu、1×107pfu、1×108Pfu or 1 × 109pfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the probiotics contains
Amount is 1 × 105cfu、1×106cfu、1×107cfu、1×108cfu、1×109Cfu or 1 × 1010cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 104Pfu, the content of probiotics are 1 × 105cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 104Pfu, the content of probiotics are 1 × 106cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 104Pfu, the content of probiotics are 1 × 107cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 104Pfu, the content of probiotics are 1 × 108cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 104Pfu, the content of probiotics are 1 × 109cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 104Pfu, the content of probiotics are 1 × 1010cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 105Pfu, the content of probiotics are 1 × 105cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 105Pfu, the content of probiotics are 1 × 106cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 105Pfu, the content of probiotics are 1 × 107cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 105Pfu, the content of probiotics are 1 × 108cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 105Pfu, the content of probiotics are 1 × 109cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 105Pfu, the content of probiotics are 1 × 1010cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 106Pfu, the content of probiotics are 1 × 105cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 106Pfu, the content of probiotics are 1 × 106cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 106Pfu, the content of probiotics are 1 × 107cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 106Pfu, the content of probiotics are 1 × 108cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 106Pfu, the content of probiotics are 1 × 109cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 106Pfu, the content of probiotics are 1 × 1010cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 107Pfu, the content of probiotics are 1 × 105cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 107Pfu, the content of probiotics are 1 × 106cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 107Pfu, the content of probiotics are 1 × 107cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 107Pfu, the content of probiotics are 1 × 108cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 107Pfu, the content of probiotics are 1 × 109cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 107Pfu, the content of probiotics are 1 × 1010cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 108Pfu, the content of probiotics are 1 × 105cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 108Pfu, the content of probiotics are 1 × 106cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 108Pfu, the content of probiotics are 1 × 107cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 108Pfu, the content of probiotics are 1 × 108cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 108Pfu, the content of probiotics are 1 × 109cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 108Pfu, the content of probiotics are 1 × 1010cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 109Pfu, the content of probiotics are 1 × 105cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 109Pfu, the content of probiotics are 1 × 106cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 109Pfu, the content of probiotics are 1 × 107cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 109Pfu, the content of probiotics are 1 × 108cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 109Pfu, the content of probiotics are 1 × 109cfu。
Further, in some schemes of the invention, in microorganism formulation described in every g, the bacteriophagic Bdellovibrio
Content be 1 × 109Pfu, the content of probiotics are 1 × 1010cfu。
Further, in some schemes of the invention, the microorganism formulation also contains carrier；
The carrier is selected from the combination of one or more of fish meal, dregs of beans and flour.
Further, in some schemes of the invention, the dosage form of the microorganism formulation is pulvis or granular pattern.
Microorganism formulation of the invention can also add carrier, and existing probiotics are often with the work such as talcum powder, wheat bran
For carrier, but talcum powder, wheat bran have apparent disadvantage, ingesting and digest bad to cultivated animals.But of the invention is micro-
Biological agent can greatly improve Tiny ecosystem using one of fish meal, dregs of beans, flour or a variety of mixing as auxiliary material or carrier
The water resistance of preparation, as feed addictive using when be added to fed in feed when, promote probiotics to be adhered to
Feed surface.Certainly, these carriers of fish meal, dregs of beans or flour are also used as solid agent, with microorganism formulation convenient for be made powder or
Particle, while playing the important effect for improving attractant.In conjunction with the respective excellent characteristics of fish meal, dregs of beans and probiotics,
Can promote cultivated animals quickly, the microorganism formulation of comprehensively ingesting, and then the microorganism for promoting it to contain is in cultivated animals body
The interior corresponding effect of performance for example inhibits pathogenic infection, improves its growth rate, survival rate and immunity of organisms, prevents and treats
Because vibrio parahaemolytious, Vibrio harveyi, luminous Vibrio, vibrio alginolyticus, Vibrio campbellii, Vibrio flurialis, Vibrio vulnificus, Aeromonas,
Disease caused by the pathogenic bacteria such as comma bacillus, pseudomonad, salmonella.
On the other hand, the present invention provides the preparation methods of described in any item microorganism formulations as above comprising: it will bite
Bacterium Bdellovibrio mixes with probiotics；
Preferably, the probiotics is selected from German-style lactobacillus subspecies bulgaricus, Lactobacillus delbrueckii subsp. lactis, excrement intestines ball
Any one in bacterium, Lactobacillus casei, lactobacillus acidophilus and lactobacillus plantarum.
Further, in some embodiments of the present invention, above-mentioned preparation method further include: by bacteriophagic Bdellovibrio and benefit
The mixture of raw bacterium is mixed with carrier.
Further, in some embodiments of the present invention, carrier is selected from one of fish meal, dregs of beans and flour or several
The combination of kind.
Simple, easily operated, the prepared microorganism system of the preparation method step of microorganism formulation provided by the invention
Agent can be used for feeding aquiculture animal such as fish, shrimp and crab, sea cucumber, shellfish etc. in aquaculture, to kill because causing
The internal pathogenic bacteria of the cultivated animals of germ infection.And the microorganism formulation has the food ration of enhancing cultivated animals, improves it
The features such as growth rate, survival rate and immunity of organisms, in addition, the microorganism formulation can also be effectively prevented and treated because of secondary haemolysis
Vibrios, Vibrio harveyi, luminous Vibrio, vibrio alginolyticus, Vibrio campbellii, Vibrio flurialis, Vibrio vulnificus, Aeromonas, comma bacillus,
Disease caused by the pathogenic bacteria such as pseudomonad, salmonella.
Further, in some embodiments of the present invention, bacteriophagic Bdellovibrio used is bacteriophagic Bdellovibrio freeze-dried powder,
Probiotics used is Freeze-dry Powder of Probioctics.
On the other hand, the present invention provides a kind of aquatic feeds comprising just like upper described in any item microorganism formulations.
Further, in some embodiments of the present invention, in the feed, the content of the microorganism formulation is
0.00002-2%.
Aquiculture animal such as fish, shrimp and crab etc. are fed using the aquatic feeds, can treat or prevent sense of causing a disease
Dye, enhances the food ration of cultivated animals, improves its growth rate, survival rate and immunity of organisms etc., while preventing and treating because of pair
Hemolysis vibrion, Vibrio harveyi, luminous Vibrio, vibrio alginolyticus, Vibrio campbellii, Vibrio flurialis, Vibrio vulnificus, Aeromonas, cholera
Disease caused by the pathogenic bacteria such as vibrios, pseudomonad, salmonella.
On the other hand, the present invention provides a kind of aquaculture methods comprising: it will described in any item microorganisms as above
Preparation, or aquatic feeds as described above feed aquiculture animal.
Wherein, aquiculture animal can be such as sea water and fresh water fish, shrimp, cray, crab, tortoise, sea cucumber, shellfish special cultivation
The combination of any one or more aquatic livestock in animal.
Using this method Aquatic farming animals such as fish, shrimp and crab etc., pathogenic infection can be effectively treated and prevented,
The food ration for enhancing cultivated animals improves its growth rate, survival rate and immunity of organisms etc., while preventing and treating because of secondary haemolysis
Vibrios, Vibrio harveyi, luminous Vibrio, vibrio alginolyticus, Vibrio campbellii, Vibrio flurialis, Vibrio vulnificus, Aeromonas, comma bacillus,
Disease caused by the pathogenic bacteria such as pseudomonad, salmonella.
It should be noted that can be added to when feeding when microorganism formulation as described above is used alone
Cultivated animals are fed in feed, naturally it is also possible to individually microorganism formulation is fed, no matter is fed in which way,
It all belongs to the scope of protection of the present invention.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment attached
Figure is briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not construed as pair
The restriction of range for those of ordinary skill in the art without creative efforts, can also be according to this
A little attached drawings obtain other relevant attached drawings.
Fig. 1 is the shape photo of the Penaeus Vannmei of the blank control group in experimental example 6.
Fig. 2 is the shape photo of the Penaeus Vannmei of the control group 1 in experimental example 6.
Fig. 3 is the shape photo of the Penaeus Vannmei of the control group 2 in experimental example 6.
Fig. 4 is the shape photo of the Penaeus Vannmei of the control group 3 in experimental example 6.
Fig. 5 is the shape photo of 201 groups of embodiment of Penaeus Vannmei in experimental example 6.
Fig. 6 is the shape photo of 202 groups of embodiment of Penaeus Vannmei in experimental example 6.
Fig. 7 is the hepatopancrease hematoxylin eosin staining result of the Penaeus Vannmei of the blank control group in experimental example 6.
Fig. 8 is the hepatopancrease hematoxylin eosin staining result of the Penaeus Vannmei of the control group 1 in experimental example 6.
Fig. 9 is the hepatopancrease hematoxylin eosin staining result of 202 groups of embodiment of Penaeus Vannmei in experimental example 6.
Figure 10 is the crab gill portion infection conditions of the blank control group in experimental example 7.
Figure 11 is the crab gill portion infection conditions of the control group 3 in experimental example 7.
Figure 12 is 238 groups of embodiment of crab gill portion infection conditions in experimental example 7.
Figure 13 is the shape photo of the cray of the blank control group in experimental example 10.
Figure 14 is the shape photo of the cray of the control group 1 in experimental example 10.
Figure 15 is the shape photo of the cray of the control group 2 in experimental example 10.
Figure 16 is the shape photo of the cray of the control group 3 in experimental example 10.
Figure 17 is the shape photo of 325 groups of embodiment of cray in experimental example 10.
Figure 18 is the shape photo of 360 groups of embodiment of cray in experimental example 10.
Specific embodiment
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, according to normal conditions or manufacturer builds
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
Feature and performance of the invention are described in further detail with reference to embodiments.
Embodiment 1
Prepare microorganism formulation
1 prepares bacteriophagic Bdellovibrio dry powder
(1) preparation of host's bacterium concentrate: picking Escherichia coli bacterium single colonie first is inoculated in nutrient broth Liquid Culture
In base (20g/L), is cultivated for 24 hours under the conditions of 150rpm, 30 DEG C, obtain culture solution；Secondly culture solution is subjected to centrifugal treating, from
Heart condition be 4000rpm, 4 DEG C, centrifugation 25min, collect sediment, with every 80ml culture solution collect precipitating 2.5ml DNB
Fluid nutrient medium suspends, and obtains host's bacterium concentrate, saves in 4 DEG C of refrigerator, for use.
Nutrient broth fluid nutrient medium used is that nutrient broth 20g/L, pH6.8,121 DEG C high pressure sterilization are standby after twenty minutes
With.
(2) preparation of bacteriophagic Bdellovibrio: according to bacteriophagic Bdellovibrio, host's bacterium concentrate: the volume ratio of DNB fluid nutrient medium
It is mixed for the ratio of 1:1:50, the condition of constant temperature incubation constant temperature incubation is 180rpm, 30 DEG C, and every 12h increases a host
Bacterium concentrate cultivates 48-72h, obtains culture solution；By medium centrifugal, nutrient broth fluid nutrient medium is nutrient broth 20g/
L, pH6.8,121 DEG C of high pressure sterilizations are spare after twenty minutes, take supernatant, filtered using 0.45 μm of cellulose acetate sheets, filtrate
Concentration is adjusted using the sterile purified water that mass volume ratio is 1.5% salinity, the concentration of bacteriophagic Bdellovibrio is made to reach 1*1010pfu/
Ml obtains bacteriophagic Bdellovibrio solution.
DNB fluid nutrient medium used, is made by the steps: weighing 0.7g/L nutrient broth, 0.45g/L casein
The yeast extract of sour hydrolysate and 0.9g/L, 15g/L sea crystal, is uniformly dissolved with distilled water, adjust pH6.8-7.3,121 DEG C,
It is saved backup after being handled 30 minutes under the conditions of 0.1MPa.
(3) freeze drying protectant is prepared: by milk powder (former 4 sections of formula milk of the tank import in Abbott Laboratories' board Europe parent Ireland, albumen
Matter 18.3%, fat 21.6%) be added suitable quantity of water dissolve after, be added sucrose, making the concentration of each component is respectively milk powder 15%w/
V, the two is adjusted pH7.8 after mixing, sterilized spare, freeze drying protectant is made by sucrose 12%w/v.
It (4) is 1:4 according to volume ratio by bacteriophagic Bdellovibrio solution obtained by step (2) and step (3) obtained freeze-drying protective agent
Ratio mixing obtains freeze-drying mixed liquor, and mixed liquor will first be lyophilized after -80 DEG C of pre-freeze 1.5h, carry out vacuum freeze drying, obtain
Bacteriophagic Bdellovibrio freeze-dried powder 1 × 1011Pfu/g is sealed under vacuum conditions, spare.
2 prepare Freeze-dry Powder of Probioctics
(1) by probiotics, that is, German-style lactobacillus subspecies bulgaricus bacterium (Lactobacillus
Delbrueckiisubspecies bulgaricus, commonly referred to as lactobacillus bulgaricus (Xactobacillm
Bulgaricm)) kind is inoculated on slant medium, is cultivated 24 hours under the conditions of 37 DEG C, is at logarithmic growth phase.
Slant medium used is MRS (g/L): glucose 20g；Tryptone 10；Beef extract 10；Yeast extract 5；
Lemon acid diamine 2；Dipotassium hydrogen phosphate 2；Bitter salt 58；4 hydrated manganese sulfates 25；Tween 80 1ml；Agar 20.
Slant medium configuration method: each ingredient other than bitter salt, four hydrated manganese sulfates and Tween 80 is molten
Solution after being cooled to 50 DEG C or less, adjusts pH6-6.5 using acetic acid, bitter salt, four hydrated manganese sulfates is then added, most
Glucose and Tween 80 are added afterwards.High pressure sterilization 20 minutes, spare under the conditions of 121 DEG C.
(2) seed of culture to logarithmic phase the preparation of suspension: is made 10 with sterile water respectively10The probiotics of cfu/ml
Suspension.
(3) prepared by fermentation liquid: the resulting probiotics suspension of step (2) is inoculated into 50L's according to 10% volume ratio
In fermentor, 48-72h, mixing speed 100rpm are cultivated under the conditions of 37 DEG C, ventilatory capacity 1vols/min controls initial pH
It is 6.8.
Slant medium of the fermentation liquor formulation such as step (1).
(4) it prepares freeze drying protectant: after milk powder addition suitable quantity of water is dissolved, sucrose is added, distinguishes the concentration of each component
For milk powder 15%w/v, sucrose 12%w/v, the two is adjusted into pH7.8 after mixing, is sterilized spare, freeze drying protectant is made.
It (5) is 1:4 ratio according to volume ratio by probiotics suspension obtained by step (2) and step (4) obtained freeze-drying protective agent
Example mixing obtains freeze-drying mixed liquor, first by after freeze-drying -80 DEG C of pre-freeze 1.5h of mixed liquor, carries out vacuum freeze drying, obtains prebiotic
(content of every kind of probiotics is different, and eventually by talcum powder as diluent, probiotics is all uniformly diluted to 1 for bacterium freeze-dried powder
×1012Cfu/g), it is sealed under the conditions of dry shady and cool, it is spare.
3 prepare microorganism formulation
Freeze-dry Powder of Probioctics, fish meal and dregs of beans made from bacteriophagic Bdellovibrio freeze-dried powder made from step 1, step 2 are mixed
It closes, microorganism formulation is made；Dosage of each component is shown in Table 1.
Embodiment 2-36
The microorganism formulation of embodiment 2-36 and the method for embodiment 1 are essentially identical, unlike, phagocytosis leech arc used
The dosage and proportion difference of bacterium freeze-dried powder, Freeze-dry Powder of Probioctics, fish meal and dregs of beans, are shown in Table 1.
Table 1
Experimental example 1
Detect the effect of the inhibition pathogenic infection of the microorganism formulation of embodiment 1-36
Experiment carries out in the Penaeus Vannmei Experimental Base in Longhai City, Fujian Province town Dong Si, totally 120 mouthfuls of ponds, Mei Gechi
18 cubic metres of the pool, 1 meter of depth of water.30 days experimental periods.Experiment is divided into 36 groups, every group of 3 repetitions, and every mouthful of pool prawn quantity is
8000 tails, prawn specification are 1cm.First group is blank control group, and control group 1 is that bacteriophagic Bdellovibrio content is 103Pfu/g+ moral
Formula lactobacillus subspecies bulgaricus 104+ 35% dregs of beans group of cfu/g+20%% fish meal, control group 2 are that bacteriophagic Bdellovibrio content is
1010Pfu/g+ German-style lactobacillus subspecies bulgaricus 1011+ 35% dregs of beans group of cfu/g+20% fish meal, control group 3 are phagocytosis leech
Vibrios content is 104Pfu/g+ normal diet group.Remaining group is experimental group, number embodiment 1-36.It is experimental group, control group 1, right
Common shrimp feed (i.e. the common shrimp feed of 20% microorganism formulation+80%) is substituted with 20% additive amount according to group 2, control group 3,
Blank control group only feeds common shrimp feed (i.e. 100% common shrimp feed), does not add any Bdellovibrio, German-style lactobacillus is protected and added
The microorganism formulations such as Leah subspecies.Pathogenic bacteria in experimentation are naturally occurrings in breeding water body, and each pond causes before experiment
Germ quantity is almost the same.The experimental results showed that the data target of each experimental group is significantly better than each control group (p ﹤ 0.05), in detail
It counts evidence accurately and is shown in Table 2.
The calculation method of pathogenic bacteria inhibiting rate: (water body pathogenic bacteria after water body pathogenic bacteria quantity (a/ml)-experiment before testing
Quantity (a/ml))/test preceding water body pathogenic bacteria quantity (a/ml)；Here what is counted is that always harmful bacterium number includes: secondary haemolysis arc
Bacterium, Vibrio harveyi, luminous Vibrio, vibrio alginolyticus, Vibrio campbellii, Vibrio flurialis, Vibrio vulnificus, Aeromonas, comma bacillus, vacation
Monad, salmonella,
The calculation method of survival rate: every mouthful of pool prawn goes out pool quantity (tail)/every mouthful of pool and puts seedling quantity (tail) * 100%
The calculation method of rate of body weight gain: (average weight (g) when prawn goes out the pool-prawn average weight (g) when putting seedling)/when putting seedling
Prawn average weight (g) * 100%.
The detection method of Phenoloxidase Activities: using catechol as substrate, phenol oxidase is measured using spectrophotometer method
Activity.Concrete operations: the TrisHCL (ph8) of addition 4.4ml, 0.2mol/L in centrifuge tube, buffer solution, 0.4ml,
The L-Pro solution of 0.5mol/L, 0.4ml, 0.5mol/L catechol and the mixing of 0.8ml crude enzyme liquid, cover centrifuge tube lid and put
After preheating 10min in 37 DEG C of water-bath, taking-up is immediately placed in ice, is terminated reaction, is poured into cuvette, with ultraviolet spectrometry light
Degree meter is scanned in 260-700nm wave-length coverage every 2nm, determines the maximum absorption wavelength (λ max) of PO effect product.
The measurement of phenol oxidase enzyme activity, the Tris-HCL (pH8) that centrifuge tube is sequentially added 4.4ml, 0.2mol/L are slow
Solution is rushed, L-PROLINE, 0.4ml, 0.5mol/L catechol of 0.4ml, 0.5mol/L are placed in 37 DEG C of water bath with thermostatic control 5min,
Then the crude enzyme liquid 0.8ml of preparatory 37 DEG C of preheatings 2min is added, which immediately sets the mixed liquor as enzyme reaction system
In cuvette, ultraviolet-visible spectrophotometer dynamics module is selected, is measured under the maximum absorption wavelength (λ max) of product
The absorbance of reaction solution, records 1 absorbance every 10s, it is quick to measure 10 altogether, and calculate PO vigor, chooses reaction system and inhales
Luminosity A ramps interval censored data and calculates enzyme activity, since this stage belongs to the first rate stage of enzymatic reaction.
Wherein, crude enzyme liquid is prepared via a method which: cold storage is solved under the conditions of -20 DEG C of whole prawn is placed in 4 DEG C
Freeze, shredded under ice bath and be ground into meat gruel, weighs 5g and 20m L Tris-HCl Extraction buffer (mass volume ratio 1: 4) is added, it is quiet
It is extracted under the conditions of being placed in 4 DEG C, then high speed refrigerated centrifuge, 4 DEG C, refrigerated centrifuge 30m under the conditions of 12 000r/m in,
Taking its supernatant is PO crude enzyme liquid.
Enzyme activity is indicated with the first rate of enzyme reaction, to increase by 0.001 per minute in managing maximum absorption wavelength (λ max)
It is defined as an enzyme activity unit, i.e. unit volume enzyme solution makes enzyme reaction system absorbance at 525nm wavelength in the unit time
Increase by 0.001 and be defined as 1 enzyme activity unit, is indicated with A/ (minmL).
Enzyme activity/(A/ (minmL))=△ A/ (0.001 × t × V).
Phenoloxidase Activities (U/mg) in table 2 are after conversion, and reaction is corresponding to the every mg weight of prawn
Phenoloxidase Activities.
In formula: △ A is absorbance change amount；T is reaction time/min；V be reaction system in be added enzyme solution volume/
ml。
Enzyme's reaction speeding (V) is indicated with the incrementss of the absorbance of A in the unit time.
V/ (A/min)=△ A/t.
Phenol oxidase is a kind of intracorporal metalloenzyme containing copper ion of shrimp, is an immune indexes of shrimp body, phenol oxygen
The content height for changing enzyme influences the ability that body resists poor environment, and the bigger expression shrimp body of Phenoloxidase Activities is extraneous micro- to resisting
The phylactic power defensive power that biology enters body is stronger, and then indicates that body is stronger to the resistance of external environment.
Table 2
Embodiment 37-72
The microorganism formulation of embodiment 37-72 and the preparation method of embodiment 1 are essentially identical, the difference is that used is prebiotic
Bacterium is Lactobacillus delbrueckii subsp. lactis (Lactobacillus delbrueckii subsp.lactis), in addition, embodiment 37-
The consumption proportion of each component in 72 microorganism formulation is shown in Table 3.
Table 3
Experimental example 2
Detect the effect of the inhibition pathogenic infection of the microorganism formulation of embodiment 37-72
Experiment carries out in the Penaeus Vannmei Experimental Base in Longhai City, Fujian Province town Dong Si, totally 120 mouthfuls of ponds, Mei Gechi
18 cubic metres of the pool, 1 meter of depth of water.30 days experimental periods.Experiment is divided into 36 groups, every group of 3 repetitions, and every mouthful of pool prawn quantity is
8000 tails, prawn specification are 1cm.First group is blank control group, and control group 1 is that bacteriophagic Bdellovibrio content is 103Pfu/g+ moral
Family name's Lactobacillus lactate subspecies 104+ 10% dregs of beans group of cfu/g+40% fish meal, control group 2 are that bacteriophagic Bdellovibrio content is 1010pfu/
G+ Lactobacillus delbrueckii subsp. lactis 1011+ 10% dregs of beans group of cfu/g+40% fish meal, control group 3 are that bacteriophagic Bdellovibrio content is
104Pfu/g+ normal diet group.Remaining group is experimental group, number embodiment 37-72.It is experimental group, control group 1, control group 2, right
Common shrimp feed is substituted with 20% additive amount according to group 3, blank control group only feeds basal feed (common shrimp feed), do not add
Add the microorganism formulations such as any Bdellovibrio, Lactobacillus delbrueckii subsp. lactis.Pathogenic bacteria in experimentation be in breeding water body from
So existing, each pond pathogenic bacteria quantity is consistent before testing.The experimental results showed that the data target index of each experimental group is significant
Better than each control group (p ﹤ 0.05), detailed data is shown in Table 4.
Table 4
Embodiment 73-108
The microorganism formulation of embodiment 73-108 and the preparation method of embodiment 1 are essentially identical, the difference is that benefit used
Raw bacterium is enterococcus faecalis (Enterococcus faecalis), in addition, each component in the microorganism formulation of embodiment 73-108
Consumption proportion be shown in Table 5.
Table 5
Experimental example 3
Detect the effect of the inhibition pathogenic infection of the microorganism formulation of embodiment 73-108
Experiment carries out in the Penaeus Vannmei Experimental Base in Longhai City, Fujian Province town Dong Si, totally 120 mouthfuls of ponds, Mei Gechi
18 cubic metres of the pool, 1 meter of depth of water.Every mouthful of pool prawn quantity is 8000 tails, and prawn specification is 1cm.30 days experimental periods.Experiment
It is divided into 36 groups, every group of 3 repetitions, first group is blank control group, and control group 1 is that bacteriophagic Bdellovibrio content is 103Pfu/g+ excrement
Enterococcus 104+ 50% dregs of beans group of cfu/g+20% fish meal, control group 2 are that bacteriophagic Bdellovibrio content is 1010Pfu/g+ enterococcus faecalis
1011+ 50% dregs of beans group of cfu/g+20% fish meal, control group 3 are that bacteriophagic Bdellovibrio content is 104Pfu/g+ normal diet group.Its
Yu Zuwei experimental group, number embodiment 73-108.Experimental group, control group 1, control group 2, control group 3 are with 20% additive amount
Common shrimp feed is substituted, blank control group only feeds basal feed (common shrimp feed), do not add any Bdellovibrio, enterococcus faecalis
Equal microorganism formulations.Pathogenic bacteria in experimentation are naturally occurrings in breeding water body, each pond pathogenic bacteria quantity before testing
Unanimously.The experimental results showed that the data target index of each experimental group is significantly better than each control group (p ﹤ 0.05), detailed data is shown in
Table 6.
Table 6
Embodiment 109-144
The microorganism formulation of embodiment 109-144 and the preparation method of embodiment 1 are essentially identical, the difference is that benefit used
Raw bacterium is Lactobacillus casei (Lactobacillus casei), in addition, each group in the microorganism formulation of embodiment 109-144
The consumption proportion divided is shown in Table 7.
Table 7
Experimental example 4
Detect the effect of the inhibition pathogenic infection of the microorganism formulation of embodiment 109-144
Experiment carries out in the Penaeus Vannmei Experimental Base in Longhai City, Fujian Province town Dong Si, totally 120 mouthfuls of ponds, Mei Gechi
18 cubic metres of the pool, 1 meter of depth of water.Every mouthful of pool prawn quantity is 8000 tails, and prawn specification is 1cm.30 days experimental periods.Experiment
It is divided into 36 groups, every group of 3 repetitions, first group is blank control group, and control group 1 is that bacteriophagic Bdellovibrio content is 103Pfu/g+ is dry
Lactobacillus paracasei 104+ 30% dregs of beans group of cfu/g+30% fish meal, control group 2 are that bacteriophagic Bdellovibrio content is 1010Pfu/g+ cheese cream
Bacillus 1011+ 30% dregs of beans group of cfu/g+30% fish meal, control group 3 are that bacteriophagic Bdellovibrio content is 104Pfu/g+ normal diet
Group.Remaining group is experimental group, number embodiment 109-144.Experimental group, control group 1, control group 2, control group 3 are with 20%
Additive amount substitutes common shrimp feed, and blank control group only feeds basal feed (common shrimp feed), does not add any Bdellovibrio, does
The microorganism formulations such as Lactobacillus paracasei.Pathogenic bacteria in experimentation are naturally occurrings in breeding water body, and each pond causes before experiment
Germ quantity is consistent.The experimental results showed that the data target index of each experimental group is significantly better than each control group (p ﹤ 0.05), in detail
It counts evidence accurately and is shown in Table 8.
Table 8
Embodiment 145-180
The preparation method of microorganism formulation and embodiment 1 that embodiment 145-180 is provided is essentially identical, the difference is that used
Probiotics be lactobacillus acidophilus (Lactobacillus acidophilus), in addition, the microorganism system of embodiment 145-180
The consumption proportion of each component in agent is shown in Table 9.
Table 9
Experimental example 5
Detect the effect of the inhibition pathogenic infection for the microorganism formulation that embodiment 145-180 is provided
Experiment carries out in the Penaeus Vannmei Experimental Base of Weifang City, Shandong Province, totally 120 mouthfuls of ponds, and each pond 20 is vertical
1 meter of depth of water of square rice.Every mouthful of pool prawn quantity is 8000 tails, and prawn specification is 1cm.30 days experimental periods.Experiment is divided into 36
Group, every group of 3 repetitions, first group is blank control group, and control group 1 is that bacteriophagic Bdellovibrio content is 103Pfu/g+ acidophilus cream bar
Bacterium 104+ 20% dregs of beans group of cfu/g+40% fish meal, control group 2 are that bacteriophagic Bdellovibrio content is 1010Pfu/g+ lactobacillus acidophilus
1011+ 20% dregs of beans group of cfu/g+40% fish meal, control group 3 are that bacteriophagic Bdellovibrio content is 104Pfu/g+ normal diet group.Its
Yu Zuwei experimental group, number embodiment 145-180.Experimental group, control group 1, control group 2, control group 3 are with 20% additive amount
Common shrimp feed is substituted, blank control group only feeds basal feed (common shrimp feed), does not add any Bdellovibrio, acidophilus cream bar
The microorganism formulations such as bacterium.Pathogenic bacteria in experimentation are naturally occurrings in breeding water body, the pathogenic bacterium number in each pond before testing
Amount is consistent.The experimental results showed that the data target index of each experimental group is significantly better than each control group (p ﹤ 0.05), detailed data
It is shown in Table 10.
Table 10
Embodiment 181-216
The preparation method of microorganism formulation and embodiment 1 that embodiment 181-216 is provided is essentially identical, the difference is that used
Probiotics be lactobacillus plantarum (Lactobacillus plantarum), in addition, the microorganism formulation of embodiment 181-216
In the consumption proportion of each component be shown in Table 11.
Table 11
Experimental example 6
(1) effect of the inhibition pathogenic infection for the microorganism formulation that detection embodiment 181-216 is provided
Experiment carries out in the Penaeus Vannmei Experimental Base of Weifang City, Shandong Province, totally 120 mouthfuls of ponds, and each pond 20 is vertical
1 meter of depth of water of square rice.Every mouthful of pool prawn quantity is 8000 tails, and prawn specification is 1cm.30 days experimental periods.Experiment is divided into 36
Group, every group of 3 repetitions, first group is blank control group, and control group 1 is that bacteriophagic Bdellovibrio content is 103Pfu/g+ plant cream bar
Bacterium 104+ 40% dregs of beans group of cfu/g+20% fish meal, control group 2 are that bacteriophagic Bdellovibrio content is 1010Pfu/g+ lactobacillus plantarum
1011+ 40% dregs of beans group of cfu/g+20% fish meal, control group 3 are that bacteriophagic Bdellovibrio content is 104Pfu/g+ normal diet group.Its
Yu Zuwei experimental group, number embodiment 181-216.Experimental group, control group 1, control group 2, control group 3 are with 20% additive amount
Common shrimp feed is substituted, blank control group only feeds basal feed (common shrimp feed), does not add any Bdellovibrio, plant cream bar
The microorganism formulations such as bacterium.Pathogenic bacteria in experimentation are naturally occurrings in breeding water body, the pathogenic bacterium number in each pond before testing
Amount is consistent.The experimental results showed that the data target index of each experimental group is significantly better than each control group (p ﹤ 0.05), detailed data
It is shown in Table 12.
Table 12
(2) shape of Penaeus Vannmei is detected
Profile measurement the result is shown in Figure 1-6, Fig. 1 of the Penaeus Vannmei of each experimental group shows the blank control of this experimental example
The shape of the Penaeus Vannmei of group shows that vigor is poor, color spot is more, jejunum is serious, growing way is slow and the features such as liver enlargement
(shape of the Penaeus Vannmei of the blank group in other experimental examples is similar)；Fig. 2 shows the south of this experimental example control group 1
The shape of penaeus vannamei, shows that vigor is promoted, stomach is small, enteron aisle is thin, growing way is general, liver has feature (other realities of reparation
The shape for testing the Penaeus Vannmei of the control group 1 in example is similar)；Fig. 3 shows that the South America of this experimental example control group 2 is white right
The shape of shrimp shows vigor promotion, feature (other experimental examples that body colour is bright, growing way is general, enteron aisle is thin, liver is clear
In control group 2 Penaeus Vannmei shape it is similar)；Fig. 4 shows the Penaeus Vannmei of this experimental example control group 3
Shape, shows that body colour is bright, growing way is general, stomach is not full, enteron aisle has jejunum, liver clearly feature (other experimental examples
In control group 3 Penaeus Vannmei shape it is similar)；Fig. 5 and Fig. 6 respectively illustrates the South America of embodiment 201 and 202
The resemblance of white shrimp shows the spy that energetic, enteron aisle is sturdy, body colour is bright, liver is clear, color spot is few, stomach is full
Sign (shape of the Penaeus Vannmei of the embodiment group in other experimental examples and the other embodiments group in this experimental example with it is such
Like).
(3) hepatopancrease of Penaeus Vannmei is detected
The colouring method for detecting hepatopancrease is hematoxylin eosin staining method: random during each test of each processing is parallel
1 tail test shrimp is taken out, complete hepatopancrease (having coating outside) is taken out from prawn carapace position using sterile tip tweezers, is placed in
It is fixed in 4% neutral formalin fixer, in case slice HE dyeing.The liver pancreas fixed is taken out from formalin fixer
Gland first uses alcohol rinse, then carries out repairing block and flushing, dehydration and transparent, saturating wax, embedding, slice (with a thickness of 4 μm), finally
HE dyeing.Ready-made histotomy is placed in optical microphotograph under the microscope, image is carried out using Olympus photomicroscope and adopts
Collection.
As a result as shown in figs. 7 to 9, Fig. 7 shows that the hepatopancrease of the Penaeus Vannmei of this experimental example blank control group occurs
Lesion (feature that the blank control group in other experimental examples behaves like), Fig. 8 show that the South America of the control group 1 of this experimental example is white
Fuller (the control group in control group 1 and control group 2-3 and this experimental example in other experimental examples of the hepatopancrease of prawn
The feature that 2-3 is behaved like)；Fig. 9 shows that the Hepatopancreas of 202 groups of embodiment of the Penaeus Vannmei of this experimental example is full
(the embodiment group in other experimental examples shows similar feature with the embodiment group in this experimental example).
Embodiment 217-252
The preparation method of microorganism formulation and embodiment 1 that embodiment 217-252 is provided is essentially identical, the difference is that used
Probiotics be lactobacillus plantarum (Lactobacillus plantarum), in addition, the microorganism formulation of embodiment 217-252
In the consumption proportion of each component be shown in Table 13.
Table 13
Experimental example 7
(1) effect of the inhibition pathogenic infection for the microorganism formulation that detection embodiment 217-252 is provided
Experiment carries out in the crab cultivation Experimental Base in Jiangsu Province Xinghua, totally 40 mouthfuls of ponds, 8 mu of each pond.One mu
Pool crab quantity is 1000, and crab specification is 5g/.210 days experimental periods.Experiment is divided into 36 groups, and each group is selected when sampling
It takes in 5, pond point (among the 4 jiaos+pond in pond), first group is blank control group, and control group 1 is that bacteriophagic Bdellovibrio content is
103Pfu/g+ lactobacillus plantarum 104+ 70% dregs of beans group of cfu/g+5% fish meal, control group 2 are that bacteriophagic Bdellovibrio content is
1010Pfu/g+ lactobacillus plantarum 1011+ 70% dregs of beans group of cfu/g+5% fish meal, control group 3 are that bacteriophagic Bdellovibrio content is
104Pfu/g+ normal diet group.Remaining group is experimental group, number embodiment 217-252.Experimental group, control group 1, control group 2,
Control group 3 substitutes common crab feed with 20% additive amount, and blank control group only feeds basal feed, and (common crab is raised
Material), the microorganism formulations such as any Bdellovibrio, lactobacillus plantarum are not added.Pathogenic bacteria in experimentation be in breeding water body from
So existing, each pond pathogenic bacteria quantity is consistent before testing.The experimental results showed that the data target index of each experimental group is significant
Better than each control group (p ﹤ 0.05), detailed data is shown in Table 14.
The detection method of superoxide dismutase: the measurement of SOD enzyme activity builds up Bioengineering Research Institute using Nanjing
One point of provided kit is measured and calculates enzyme activity.
Superoxides gasification enzyme is a kind of important antioxidase, is that active oxygen participates in removing intracorporal free radical.SOD enzyme
For defencive function macromolecular will not Oxidative demage play an important role.SOD enzyme activity is bigger, shows the immunocompetence of body
Better, SOD enzyme activity is smaller, shows that the immunocompetence of body is poorer.
Table 14
(2) crab gill portion infection conditions are detected by anatomy method, result figure 10- Figure 12, Figure 10 show this experimental example
In blank control group crab gill portion infection conditions, infected with pathogenic bacteria, oedema is serious；Figure 11 is shown in this experimental example
Control group 3 crab gill portion infection conditions, show general infection, but enteron aisle is thinner, (this reality that food filler is insufficient
The control group 1-2 for testing example shows similar feature)；Figure 12 shows 238 groups of the embodiment in this experimental example of crab
Gill portion infection conditions show the cleaning of crab gill portion, enteron aisle is clear, food is full (the other embodiments group table of this experimental example
Reveal similar feature).
Embodiment 253-288
The preparation method of microorganism formulation and embodiment 1 that embodiment 253-288 is provided is essentially identical, the difference is that used
Probiotics be lactobacillus acidophilus (Lactobacillus acidophilus), in addition, the microorganism system of embodiment 253-288
The consumption proportion of each component in agent is shown in Table 15.
Table 15
Experimental example 8
Detect the effect of the inhibition pathogenic infection for the microorganism formulation that embodiment 253-288 is provided
Experiment carries out in the cray culture experiment base of Qianjiang City, Hubei Province, totally 40 mouthfuls of ponds, 10 mu of each pond.
It is 5000 that one mu of pool cray, which averagely puts seedling quantity, and cray specification is 5g.30 days experimental periods.Experiment is divided into 36 groups, takes
It is chosen among pond quadrangle+pond when sample, totally 5 points, first group is blank control group, and control group 1 is bacteriophagic Bdellovibrio content
It is 103Pfu/g+ lactobacillus plantarum 104+ 78% dregs of beans group of cfu/g+1% fish meal, control group 2 are that bacteriophagic Bdellovibrio content is
1010Pfu/g+ lactobacillus plantarum 1011+ 78% dregs of beans group of cfu/g+1% fish meal, control group 3 are that bacteriophagic Bdellovibrio content is
104Pfu/g+ normal diet group.Remaining group is experimental group, number embodiment 253-288.Experimental group, control group 1, control group 2,
Control group 3 substitutes common cray feed with 20% additive amount, and blank control group only feeds basal feed (common cray
Feed), the microorganism formulations such as any Bdellovibrio, lactobacillus plantarum are not added.Pathogenic bacteria in experimentation are in breeding water body
Naturally occurring, each pond pathogenic bacteria quantity is consistent before testing.The experimental results showed that the data target index of each experimental group is aobvious
It writes and is better than each control group (p ﹤ 0.05), detailed data is shown in Table 16.
Table 16
Embodiment 289-324
The preparation method for the microorganism formulation that embodiment 289-324 is provided is general preparative methods in industry, embodiment
Probiotics used in 289-324 is bacillus subtilis (Bacillus subtilis), other are same as Example 1, in addition,
The consumption proportion of each component in the microorganism formulation of embodiment 289-324 is shown in Table 17.
Table 17
Experimental example 9
Detect the effect of the inhibition pathogenic infection for the microorganism formulation that embodiment 289-324 is provided
Experiment carries out in the cray culture experiment base of Qianjiang City, Hubei Province, totally 40 mouthfuls of ponds, 10 mu of each pond.
It is 5000 that one mu of pool cray, which averagely puts seedling quantity, and cray specification is 5g.30 days experimental periods.Experiment is divided into 36 groups, takes
It is chosen among pond quadrangle+pond when sample, totally 5 points, first group is blank control group, and control group 1 is bacteriophagic Bdellovibrio content
It is 103Pfu/g+ bacillus subtilis 104+ 78% dregs of beans group of cfu/g+1% fish meal, control group 2 are that bacteriophagic Bdellovibrio content is
1010Pfu/g+ bacillus subtilis 1011+ 78% dregs of beans group of cfu/g+1% fish meal, control group 3 are that bacteriophagic Bdellovibrio content is
104Pfu/g+ normal diet group.Remaining group is experimental group, number embodiment 289-324.Experimental group, control group 1, control group 2,
Control group 3 substitutes common cray feed with 20% additive amount, and blank control group only feeds basal feed (common cray
Feed), the microorganism formulations such as any Bdellovibrio, bacillus subtilis are not added.Pathogenic bacteria in experimentation are breeding water bodies
Middle naturally occurring, each pond pathogenic bacteria quantity is consistent before testing.The experimental results showed that the data target index of each experimental group
It is significantly better than each control group (p ﹤ 0.05), detailed data is shown in Table 18.
Table 18
Embodiment 325-360
The preparation method for the microorganism formulation that embodiment 325-360 is provided is general preparative methods in industry, embodiment
Probiotics used in 325-360 is bacillus licheniformis (Bacillus licheniformis), other are same as Example 1,
In addition, the consumption proportion of each component in the microorganism formulation of embodiment 325-360 is shown in Table 19.
Table 19
Experimental example 10
Detect the effect of the inhibition pathogenic infection for the microorganism formulation that embodiment 325-360 is provided
Experiment carries out in the cray culture experiment base of Jiangsu Province Lianyungang, totally 40 mouthfuls of ponds, each pond 8
Mu.It is 5000 that one mu of pool cray, which averagely puts seedling quantity, and cray specification is 5g.30 days experimental periods.Experiment is divided into 36 groups,
It is chosen among pond quadrangle+pond when sampling, totally 5 points, first group is blank control group, and control group 1 contains for bacteriophagic Bdellovibrio
Amount is 103Pfu/g+ bacillus licheniformis 104+ 78% dregs of beans group of cfu/g+1% fish meal, control group 2 are that bacteriophagic Bdellovibrio content is
1010Pfu/g+ bacillus licheniformis 1011+ 78% dregs of beans group of cfu/g+1% fish meal, control group 3 are that bacteriophagic Bdellovibrio content is
104Pfu/g+ normal diet group.Remaining group is experimental group, number embodiment 325-360.Experimental group, control group 1, control group 2,
Control group 3 substitutes common cray feed with 20% additive amount, and blank control group only feeds basal feed (common cray
Feed), the microorganism formulations such as any Bdellovibrio, bacillus licheniformis are not added.Pathogenic bacteria in experimentation are breeding water bodies
Middle naturally occurring, each pond pathogenic bacteria quantity is consistent before testing.The experimental results showed that the data target index of each experimental group
It is significantly better than each control group (p ﹤ 0.05), detailed data is shown in Table 20.
Table 20
(2) shape of cray is detected
Profile measurement the result is shown in Figure 1 3-18, Figure 13 of the cray of each experimental group show the blank control of this experimental example
The shape of the cray of group, the bending of cray body, body muscle is not full, and vigor is poor, and crust is dim, and food ration is few, growing way
Poor (shape of the cray of the blank group in other experimental examples is similar)；Figure 14 shows this experimental example control group 1
The shape of cray, cray body are unfolded, and vigor is preferable, and body muscle is fuller, and crust is bright, and food ration is general, growing way
Generally (shape of the cray of the control group 1 in other experimental examples is similar)；Figure 15 shows this experimental example control group 2
The shape of cray shows cray body and unfolds, and energetic, with muscle-bound, four limbs are strong, and crust is bright, and muscle is full
Full, food ration is larger (shape of the cray of the control group 2 in other experimental examples is similar)；Figure 16 shows this experimental example
The shape of the cray of control group 3, show cray body unfold, but crust it is smaller, growing way is general, and body is partially thin,
Food ration is general, vigor deficiency (shape of the cray of the control group 3 in other experimental examples is similar)；Figure 17 shows reality
The resemblance for applying 325 groups of example of cray shows cray crust light, four limbs stalwartness, and physical health, muscle are full
Full, vigor is good, and food ration is big.Figure 18 shows the resemblance of 360 groups of embodiment of cray, shows cray body
Unfold, crust light, physical health, muscle enrich, four limbs are healthy and strong, and vigor is good, and food ration is good.(the implementation in other experimental examples
The shape of the cray of example group and the other embodiments group in this experimental example is similar).
Embodiment 361-396
The preparation method for the microorganism formulation that embodiment 361-396 is provided is general preparative methods in industry, embodiment
Probiotics used in 361-396 is bacillus coagulans (Bacillus coagulans), other are same as Example 1, in addition,
The consumption proportion of each component in the microorganism formulation of embodiment 361-396 is shown in Table 21.
Table 21
Experimental example 11
Detect the effect of the inhibition pathogenic infection for the microorganism formulation that embodiment 361-396 is provided
Experiment carries out in the cray culture experiment base of Jiangsu Province Lianyungang, totally 40 mouthfuls of ponds, each pond 8
Mu.It is 5000 that one mu of pool cray, which averagely puts seedling quantity, and cray specification is 5g.30 days experimental periods.Experiment is divided into 36 groups,
It is chosen among pond quadrangle+pond when sampling, totally 5 points, first group is blank control group, and control group 1 contains for bacteriophagic Bdellovibrio
Amount is 103Pfu/g+ bacillus coagulans 104+ 78% dregs of beans group of cfu/g+1% fish meal, control group 2 are that bacteriophagic Bdellovibrio content is
1010Pfu/g+ bacillus coagulans 1011+ 78% dregs of beans group of cfu/g+1% fish meal, control group 3 are that bacteriophagic Bdellovibrio content is
104Pfu/g+ normal diet group.Remaining group is experimental group, number embodiment 361-396.Experimental group, control group 1, control group 2,
Control group 3 substitutes common cray feed with 20% additive amount, and blank control group only feeds basal feed (common cray
Feed), the microorganism formulations such as any Bdellovibrio, bacillus coagulans are not added.Pathogenic bacteria in experimentation are breeding water bodies
Middle naturally occurring, each pond pathogenic bacteria quantity is consistent before testing.The experimental results showed that the data target index of each experimental group
It is significantly better than each control group (p ﹤ 0.05), detailed data is shown in Table 22.
Table 22
Embodiment 397-432
The preparation method for the microorganism formulation that embodiment 397-432 is provided is general preparative methods in industry, embodiment
Probiotics used in 397-432 is bacillus pumilus (Bacillus pumilus), other are same as Example 1, in addition, real
The consumption proportion for applying each component in the microorganism formulation of a 397-432 is shown in Table 23.
Table 23
Experimental example 12
Detect the effect of the inhibition pathogenic infection for the microorganism formulation that embodiment 397-432 is provided
Experiment carries out in the cray culture experiment base of Jiangsu Province Lianyungang, totally 40 mouthfuls of ponds, each pond 8
Mu.It is 5000 that one mu of pool cray, which averagely puts seedling quantity, and cray specification is 5g.30 days experimental periods.Experiment is divided into 36 groups,
It is chosen among pond quadrangle+pond when sampling, totally 5 points, first group is blank control group, and control group 1 contains for bacteriophagic Bdellovibrio
Amount is 103Pfu/g+ bacillus pumilus 104+ 78% dregs of beans group of cfu/g+1% fish meal, control group 2 are that bacteriophagic Bdellovibrio content is
1010Pfu/g+ bacillus pumilus 1011+ 78% dregs of beans group of cfu/g+1% fish meal, control group 3 are that bacteriophagic Bdellovibrio content is
104Pfu/g+ normal diet group.Remaining group is experimental group, number embodiment 397-432.Experimental group, control group 1, control group 2,
Control group 3 substitutes common cray feed with 20% additive amount, and blank control group only feeds basal feed (common cray
Feed), the microorganism formulations such as any Bdellovibrio, bacillus pumilus are not added.Pathogenic bacteria in experimentation are breeding water bodies
Middle naturally occurring, each pond pathogenic bacteria quantity is consistent before testing.The experimental results showed that the data target index of each experimental group
It is significantly better than each control group (p ﹤ 0.05), detailed data is shown in Table 24.
Table 24
Embodiment 433-468
The preparation method for the microorganism formulation that embodiment 433-468 is provided is general preparative methods in industry, embodiment
Probiotics used in 433-468 is bacillus laterosporus (Bacillus laterosporu), other are same as Example 1, separately
Outside, the consumption proportion of each component in the microorganism formulation of embodiment 433-468 is shown in Table 25.
Table 25
Experimental example 13
Detect the effect of the inhibition pathogenic infection for the microorganism formulation that embodiment 433-468 is provided
Experiment carries out in the cray culture experiment base in Xuyi city, Jiangsu Province, totally 40 mouthfuls of ponds, 10 mu of each pond.
It is 5000 that one mu of pool cray, which averagely puts seedling quantity, and cray specification is 5g.30 days experimental periods.Experiment is divided into 36 groups, takes
It is chosen among pond quadrangle+pond when sample, totally 5 points, first group is blank control group, and control group 1 is bacteriophagic Bdellovibrio content
It is 103Pfu/g+ bacillus laterosporus 104+ 78% dregs of beans group of cfu/g+1% fish meal, control group 2 are that bacteriophagic Bdellovibrio content is
1010Pfu/g+ bacillus laterosporus 1011+ 78% dregs of beans group of cfu/g+1% fish meal, control group 3 are that bacteriophagic Bdellovibrio content is
104Pfu/g+ normal diet group.Remaining group is experimental group, number embodiment 433-468.Experimental group, control group 1, control group 2,
Control group 3 substitutes common cray feed with 20% additive amount, and blank control group only feeds basal feed (common cray
Feed), the microorganism formulations such as any Bdellovibrio, bacillus laterosporus are not added.Pathogenic bacteria in experimentation are breeding water bodies
Middle naturally occurring, each pond pathogenic bacteria quantity is consistent before testing.The experimental results showed that the data target index of each experimental group
It is significantly better than each control group (p ﹤ 0.05), detailed data is shown in Table 26.
Table 26
Embodiment 469-504
The preparation method for the microorganism formulation that embodiment 469-504 is provided is general preparative methods in industry, embodiment
Probiotics used in 469-504 is clostridium butyricum (Clostridium butyricum), other are same as Example 1, in addition,
The consumption proportion of each component in the microorganism formulation of embodiment 469-504 is shown in Table 27.
Table 27
Experimental example 14
Detect the effect of the inhibition pathogenic infection for the microorganism formulation that embodiment 469-504 is provided
Experiment carries out in the cray culture experiment base of Qianjiang City, Hubei Province, totally 40 mouthfuls of ponds, 10 mu of each pond.
It is 5000 that one mu of pool cray, which averagely puts seedling quantity, and cray specification is 5g.30 days experimental periods.Experiment is divided into 36 groups, takes
It is chosen among pond quadrangle+pond when sample, totally 5 points, first group is blank control group, and control group 1 is bacteriophagic Bdellovibrio content
It is 103Pfu/g+ clostridium butyricum 104+ 78% dregs of beans group of cfu/g+1% fish meal, control group 2 are that bacteriophagic Bdellovibrio content is
1010Pfu/g+ clostridium butyricum 1011+ 78% dregs of beans group of cfu/g+1% fish meal, control group 3 are that bacteriophagic Bdellovibrio content is 104pfu/
G+ normal diet group.Remaining group is experimental group, number embodiment 469-504.Experimental group, control group 1, control group 2, control group 3
Common cray feed is substituted with 20% additive amount, blank control group only feeds basal feed (common cray feed),
The microorganism formulations such as any Bdellovibrio, clostridium butyricum are not added.Pathogenic bacteria in experimentation are naturally occurrings in breeding water body
, each pond pathogenic bacteria quantity is consistent before testing.The experimental results showed that the data target index of each experimental group is significantly better than respectively
Control group (p ﹤ 0.05), detailed data is shown in Table 28.
Table 28
Embodiment 505-540
The preparation method for the microorganism formulation that embodiment 505-540 is provided is general preparative methods in industry, embodiment
Probiotics used in 505-540 is Rhodopseudomonas palustris (Rhodop seudomonas palustris), other and embodiment
1 is identical, in addition, the consumption proportion of each component in the microorganism formulation of embodiment 505-540 is shown in Table 29.
Table 29
Experimental example 15
Detect the effect of the inhibition pathogenic infection for the microorganism formulation that embodiment 505-540 is provided
Experiment carries out in the cray culture experiment base in Xuyi city, Jiangsu Province, totally 40 mouthfuls of ponds, 10 mu of each pond.
It is 5000 that one mu of pool cray, which averagely puts seedling quantity, and cray specification is 5g.30 days experimental periods.Experiment is divided into 36 groups, takes
It is chosen among pond quadrangle+pond when sample, totally 5 points, first group is blank control group, and control group 1 is bacteriophagic Bdellovibrio content
It is 103Pfu/g+ Rhodopseudomonas palustris 104+ 78% dregs of beans group of cfu/g+1% fish meal, control group 2 are that bacteriophagic Bdellovibrio content is
1010Pfu/g+ Rhodopseudomonas palustris 1011+ 78% dregs of beans group of cfu/g+1% fish meal, control group 3 are that bacteriophagic Bdellovibrio content is
104Pfu/g+ normal diet group.Remaining group is experimental group, number embodiment 505-540.Experimental group, control group 1, control group 2,
Control group 3 substitutes common cray feed with 20% additive amount, and blank control group only feeds basal feed (common cray
Feed), the microorganism formulations such as any Bdellovibrio, Rhodopseudomonas palustris are not added.Pathogenic bacteria in experimentation are cultivation waters
Naturally occurring in body, each pond pathogenic bacteria quantity is consistent before testing.The experimental results showed that the data target of each experimental group refers to
Mark is significantly better than each control group (p ﹤ 0.05), and detailed data is shown in Table 30.
Table 30
It in summary it can be seen, microorganism formulation provided in an embodiment of the present invention passes through bacteriophagic Bdellovibrio and probiotics such as moral
Formula lactobacillus subspecies bulgaricus, Lactobacillus delbrueckii subsp. lactis, enterococcus faecalis, Lactobacillus casei, lactobacillus acidophilus, withered grass bud
Spore bacillus, bacillus licheniformis, bacillus pumilus, bacillus laterosporus, bacillus coagulans, clostridium butyricum, the red vacation in marsh
After monad or lactobacillus plantarum scientific compatibility, then compound fish meal, dregs of beans and using flour or Strong flour as specific support, with
20% or more additive amount uses in prawn, crab, cray, fish meal, can obviously reduce the intracorporal liver of cultivated animals, intestines
The pathogenic bacteria quantity in road and water body, while cultivated animals can be promoted to promote it to ingest the digestion of feed.The microorganism formulation
Has the function of obvious alternative antibiotic performance.In addition, it compared with the feed addictive of similar functions, has and more pacifies
Entirely, efficiently, noresidue, nontoxic pair advantage.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of microorganism formulation, which is characterized in that it includes bacteriophagic Bdellovibrio and probiotics；
Preferably, the probiotics be selected from German-style lactobacillus subspecies bulgaricus, Lactobacillus delbrueckii subsp. lactis, enterococcus faecalis,
Lactobacillus casei, lactobacillus acidophilus, Rhodopseudomonas palustris, clostridium butyricum, bacillus laterosporus, bacillus pumilus, condensation
Any one in bacillus, bacillus licheniformis, bacillus subtilis and lactobacillus plantarum.
2. microorganism formulation according to claim 1, which is characterized in that the work of the bacteriophagic Bdellovibrio and the probiotics
Bacterium number ratio is (1-104):(1-106)。
3. microorganism formulation according to claim 2, which is characterized in that the work of the bacteriophagic Bdellovibrio and the probiotics
Bacterium number ratio is 1:(1-106)；
Preferably, the bacteriophagic Bdellovibrio and the viable count ratio of the probiotics are 1:1,1:10,1:100,1:1000,1:
10000,1:100000 or 1:1000000.
4. microorganism formulation according to claim 2, which is characterized in that the work of the bacteriophagic Bdellovibrio and the probiotics
Bacterium number ratio is (1-104):1；
Preferably, the bacteriophagic Bdellovibrio and the viable count ratio of the probiotics are 10:1,100:1,1000:1 or 10000:1.
5. microorganism formulation according to claim 1-4, which is characterized in that in microorganism formulation described in every g, institute
The content for stating bacteriophagic Bdellovibrio is 1 × 104-1×109In microorganism formulation described in pfu, every g, the content of the probiotics is 1 ×
105-1×1010cfu。
6. according to the described in any item microorganism formulations of claim 5, which is characterized in that the microorganism formulation also contains load
Body；
The carrier is selected from the combination of one or more of fish meal, dregs of beans and flour.
7. microorganism formulation according to claim 6, which is characterized in that the dosage form of the microorganism formulation be pulvis or
Grain shape.
8. such as the preparation method of the described in any item microorganism formulations of claim 1-7, characterized in that it comprises: by phagocytosis
Bdellovibrio mixes with probiotics；
Preferably, the probiotics be selected from German-style lactobacillus subspecies bulgaricus, Lactobacillus delbrueckii subsp. lactis, enterococcus faecalis,
Any one in Lactobacillus casei, lactobacillus acidophilus and lactobacillus plantarum.
9. a kind of aquatic feeds, which is characterized in that it includes the described in any item microorganism formulations of claim 1-7.
10. a kind of aquaculture method, characterized in that it comprises: by the described in any item microorganism systems of claim 1-7
Agent or aquatic feeds as claimed in claim 9 feed aquiculture animal.
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