CN109557027B - Automatic analysis device and detection method for container state in working process thereof - Google Patents
Automatic analysis device and detection method for container state in working process thereof Download PDFInfo
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Abstract
The invention relates to an automatic analysis device and a detection method of container state in the working process thereof, wherein the method comprises the following steps: acquiring a first threshold value of the optical sensor in a non-shielding state, a second threshold value of the optical sensor in a full-shielding state and a third threshold value of the optical sensor in an empty container shielding state; moving the container containing the test solution to a position aligned with the optical sensor to obtain a first detection value; judging whether the first detection value is between the first threshold value and the second threshold value after redundancy correction and is within a liquid adding range of a third threshold value, if so, judging that the container is normally loaded, otherwise, judging that the container is failed to be loaded; after the test solution in the container is processed, the optical sensor obtains a second detection value; and judging whether the difference value between the second detection value and the first detection value is larger than the error range, if so, judging that the container processing is normal, and otherwise, judging that the container processing is abnormal. The invention adopts the mode of combining non-contact, sectional type and dynamic detection of the container state by the optical sensor, thereby improving the detection accuracy.
Description
Technical Field
The present invention relates to the technical field of testing or analyzing materials by means of determining chemical or physical properties of the materials, and in particular to an automatic analysis device and a method for detecting the state of a container during operation of the automatic analysis device.
Background
Automatic analyzers, such as fully automated chemiluminescent diagnostic instruments, require multiple washes of the reagents to remove free material after the sample has undergone an immunoreaction. The state of the reaction cup in the cleaning process comprises: the conditions of normal loading reaction cup, abnormal loading reaction cup, normal liquid pumping, abnormal liquid pumping, normal liquid injection, abnormal liquid injection and the like. And the liquid property in the reaction cup is changed in the cleaning process, and the main components comprise: "serum", "magnetic beads", "reagents", "wash", and the like; in the cleaning process, the concentration ratios of the various substances can be changed continuously, so that the characteristics of the test solution in the reaction cup are changed, and the state detection of the reaction cup is difficult. For example, in the technique of detecting the state of the cuvette using the optical sensor, the concentration, viscosity, reagent characteristics, and the like of the sample solution in the cuvette are unexpectedly and visually changed, and the absorbance is also changed due to the change, thereby affecting the judgment of the optical sensor.
Disclosure of Invention
In view of the above, it is desirable to provide an automatic analyzer capable of accurately detecting the state of a container during the operation of the automatic analyzer, and a method for detecting the state of a container during the operation of the automatic analyzer.
A detection method for the container state in the working process of an automatic analysis device comprises a modeling stage and a working detection stage after the modeling stage, wherein the modeling stage comprises the following steps: acquiring a first threshold value of light intensity received by a light sensor in a corresponding non-shielding state, and acquiring a second threshold value of light intensity received by the light sensor in a corresponding full-shielding state; obtaining a third threshold value according to the light intensity received by the light sensor in the state of being shielded by the empty container; the in-service detection phase comprises: the automatic analysis device moves the container containing the experimental substance to a position aligned with the optical sensor, and a first detection value is obtained according to the light intensity received by the optical sensor at the moment; judging whether the first detection value simultaneously meets two conditions: the liquid filling range is between the first threshold value after the redundancy correction and the second threshold value after the redundancy correction and is within the liquid filling range of the third threshold value; if the two conditions are met simultaneously, judging that the container is normally loaded, otherwise, judging that the container is failed to be loaded; after the automatic analysis device processes the experimental substance in the container, a second detection value is obtained according to the light intensity received by the light sensor at the moment; judging whether the difference value between the second detection value and the first detection value is larger than the error range, if so, judging that the container processing is normal, otherwise, judging that the container processing is abnormal; the absolute value of the error range is smaller than the absolute value of the liquid adding range.
In one embodiment, the automated analysis device processes the test substance in the container, including the automated analysis device removing the test substance from the container.
In one embodiment, the automated analysis device processes the test substance in the container, including the automated analysis device performing a substance infusion process on the container after removing the test substance from the container; the second detection value is obtained after the experimental substance is removed from the container and before the substance injection treatment is carried out; the in-service detection phase further comprises: after the injection treatment of the substance, obtaining a third detection value according to the light intensity received by the light sensor at the moment; and judging whether the difference value of the third detection value and the second detection value is larger than the error range, if so, judging that the material injection treatment is normal, otherwise, judging that the material injection treatment is abnormal.
In one embodiment, the automatic analysis device comprises a carousel provided with a plurality of housing positions for housing the containers, each housing position being intended to house one of the containers; when the containers are arranged on the rotary disc, gaps exist between the adjacent containers, and a blocking piece between the containers is arranged at least one gap; the in-service detection phase further comprises: the turntable rotates to align the gap with the position of the optical sensor, and a fourth detection value is obtained according to the light intensity received by the optical sensor at the moment; the turntable rotates to a position where the gap is aligned with the separation blade between the containers, and a fifth detection value is obtained according to the light intensity received by the light sensor at the moment; and if the fourth detection value is in the redundancy range of the first threshold value and the fifth detection value is in the redundancy range of the second threshold value, judging that the optical sensor is normal.
In one embodiment, the performing the substance injection process is adding a cleaning solution to the container.
In one embodiment, the automatic analysis device comprises a carousel provided with a plurality of housing positions for housing the containers, each housing position being intended to house one of the containers; when the containers are arranged on the turntable, a gap exists between every two adjacent containers; the in-service detection phase further comprises: the turntable rotates to align the gap with the position of the optical sensor, and a fourth detection value is obtained according to the light intensity received by the optical sensor at the moment; setting a blocking piece, rotating the turntable to a position where the optical sensor is aligned with the blocking piece, and obtaining a fifth detection value according to the light intensity received by the optical sensor at the moment; and if the fourth detection value is in the redundancy range of the first threshold value and the fifth detection value is in the redundancy range of the second threshold value, judging that the optical sensor is normal.
In one embodiment, the light sensor is a reflective light coupler or a counter-reflective light coupler; the modeling stage also comprises a step of subtracting the first threshold from the second threshold and dividing the subtracted result by a scale coefficient to obtain a scale threshold, wherein the scale coefficient is a natural number which is greater than 2 and smaller than 100; the time between the first threshold value after the redundancy correction and the second threshold value after the redundancy correction is greater than the first threshold value plus scale threshold value and less than the second threshold value minus scale threshold value; the liquid adding range in the third threshold is larger than the third threshold minus the scale threshold and smaller than the third threshold plus the scale threshold; the absolute value of the error range is equal to the calibration threshold.
In one embodiment, the scale factor is 16.
In one embodiment, the obtaining of the second threshold corresponding to the light intensity received by the light sensor in the full shielding state is to align the light sensor with a white baffle, and obtain the second threshold according to the light intensity received by the light sensor.
In one embodiment, the container is a translucent container.
An automatic analysis device comprises a control system, wherein the control system is used for executing the detection method of the container state in the working process of the automatic analysis device.
According to the automatic analysis device and the detection method of the container state in the working process of the automatic analysis device, the optical sensor is used for carrying out non-contact detection on the container state, and the problem that a sample is possibly polluted in the detection process is solved. By adopting a mode of combining sectional detection and dynamic detection, firstly establishing a three-section model of no-shielding/empty container shielding/full shielding through a modeling stage, thereby detecting various states including normal container loading, abnormal container loading, normal container processing and abnormal container processing; the first detection value is obtained according to the container containing the experimental substance in the detection stage, whether the subsequent container processing is normal or not is judged according to the comparison with the first detection value, and the dynamic detection can overcome the error caused by the change of the characteristics of the experimental substance in the container.
Drawings
FIG. 1 is a flow chart illustrating a method for detecting a container status during operation of an automated analyzer in one embodiment.
Detailed Description
To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
It should be understood that, although the steps in the flowchart of the present invention are shown in order as indicated by the arrows, the steps are not necessarily performed in order as indicated by the arrows. The steps are not performed in the exact order shown and may be performed in other orders unless explicitly stated herein. Moreover, at least a part of steps in the flowcharts of the present invention may include multiple sub-steps or multiple stages, which are not necessarily performed at the same time, but may be performed at different times, and the order of performing may not necessarily be sequential, but may be performed alternately or alternately with other steps or at least a part of sub-steps or stages of other steps.
Fig. 1 is a flow chart of a method for detecting the state of a container during the operation of an automatic analyzer, the detection includes a modeling phase P1 and an in-operation detection phase P2, and the following steps are specifically described:
s110, a first threshold value of the optical sensor in a non-shielding state and a second threshold value of the optical sensor in a full-shielding state are obtained.
In the detection method of the present embodiment, the transmittance of a container (for example, a cuvette, a sample cup, or the like) used in the operation of an automatic analyzer is measured by an optical sensor to determine the container state. In this step, a first threshold corresponding to the light intensity received by the light sensor in the non-shielding state is obtained, and a second threshold corresponding to the light intensity received by the light sensor in the full-shielding state is obtained. The second threshold value is a value which can ensure that the light intensity received by the light sensor is between a non-shielding state and a full-shielding state when any experimental material which can enter the container in the working process of the automatic analysis device is contained in the container. Namely, the first threshold value and the second threshold value are set, so that when any experimental substance is contained in the container, the detection value is between the first threshold value and the second threshold value.
The first threshold and the second threshold may be actually measured or may be set empirically.
In one embodiment, the measurement of the second threshold for the full block condition is performed using a white shutter. And aligning the white baffle to the optical sensor, simulating the condition that the optical sensor is completely shielded, and obtaining a second threshold value according to the light intensity received by the optical sensor.
In one embodiment, the automatic analysis device comprises a carousel provided with a plurality of housing positions for housing said containers, each housing position being intended to house one container. When the containers are arranged on the rotating disc, gaps exist between the adjacent containers. The first threshold may be measured by controlling the turntable to move to a position where the light sensor is aligned with the gap between adjacent containers, the first threshold being derived from the intensity of light received by the light sensor.
The light sensor converts the received light intensity into a value that is convenient for automatic processing, for example, for the light sensor is a photoelectric sensor, the received light intensity is converted into an electric quantity (voltage value), and therefore, in the corresponding embodiment, the first threshold and the second threshold are voltage values.
The optical sensor comprises two types, one type is a reflection type, namely, an optical transmitter and an optical receiver are arranged on the same side of a measured object, and light rays emitted by the optical transmitter are reflected back to the optical receiver by the measured object; the other type is a separation type, namely, the light receiver is independently arranged, the light receiver is arranged on the other side of the measured object or directly uses external light, and the received light intensity of the light receiver can be reduced when the light receiver is shielded by an object. Specifically, which optical sensor is applied to the above detection method in the embodiment may be selected according to the specific structure of the applied automatic analyzer, for example, according to the specific structure size of each optical sensor, considering whether the automatic analyzer has enough space for installation, whether light is interfered by other components, and the like.
S120, a third threshold value of the optical sensor in a state of being blocked by an empty container is obtained.
The third threshold is obtained from the intensity of light received by the light sensor in a state blocked by an empty container. It can be understood that, since different containers have different light transmittance, the corresponding container is configured on the automatic analyzer in this embodiment, and the third threshold is obtained by actual measurement. That is, what container is actually used when the automatic analyzer is in operation, the third threshold value is actually measured by the container. In one example, the cuvette material is translucent ethylene. The obtaining sequence of the first, second and third threshold values has no requirement in sequence, and can be performed simultaneously or sequentially according to any sequence.
The steps S110 and S120 belong to the modeling stage P1, and the automatic analysis device can be started to perform actual work after the modeling is completed. The values obtained during the modeling phase P1 can be recorded and stored, including the first, second and third threshold values, so that the automatic analysis device does not need to model again in future operations.
S130, the optical sensor is aligned to a container containing the experimental material to obtain a first detection value.
The automatic analysis device moves the container containing the experimental substance to the position aligned with the optical sensor, and a first detection value is obtained according to the light intensity received by the optical sensor at the moment. The test substance may be a reagent, a sample (typically a biological fluid), a wash solution, magnetic beads, or the like.
S140, determining whether the first detection value satisfies two conditions: and the liquid filling range is between the first threshold value after the redundancy correction and the second threshold value after the redundancy correction and is within the liquid filling range of the third threshold value.
If the two conditions are met simultaneously, judging that the container is loaded normally; otherwise, judging that the container is loaded abnormally.
When the container is loaded normally, a first detection value corresponding to the light intensity received by the light sensor is between a first threshold value and a second threshold value. It will be appreciated that the first detection value is also anomalous if it is very close to the first threshold value or the second threshold value. Therefore, when the first detection value is judged to fall into the interval, redundancy correction is carried out on the interval between the first threshold value and the second threshold value, a cell interval which belongs to the interval and is narrower than the interval is obtained, and judgment is carried out according to whether the first detection value falls into the cell interval or not. The cell interval obtained after redundancy correction should be greater than half of the interval between the first threshold and the second threshold. On the other hand, the transmittance of the container containing the experimental substance is close to that of the empty container, so that whether the container is normally loaded can be judged according to whether the first detection value is in the liquid adding range of the third threshold value. The liquid adding range is a range which is beyond the range, namely the light transmittance is judged to be obviously inconsistent with the container containing the experimental substance, and the difference between the upper limit value and the lower limit value of the liquid adding range is smaller than the absolute value of the difference between the third threshold value and the first threshold value and smaller than the absolute value of the difference between the second threshold value and the first threshold value.
It can be understood that if the container is loaded abnormally, the subsequent processing of the automatic analysis device will be in error, and therefore, the automatic analysis device continues to operate only when the container is loaded normally, and the detection method of the embodiment also continues to follow the subsequent flow, and then the step S150 is performed.
S150, the experimental substance in the container is processed, and the optical sensor obtains a second detection value.
And after the automatic analysis device processes the experimental substance in the container, a second detection value is obtained according to the light intensity received by the light sensor at the moment.
In this embodiment, step S150 is to move the test substance out of the container by the automatic analyzer, which may be to move a part of the test substance out of the container, to keep a part of the test substance in the container, or to move all of the test substance out of the container. It is understood that more than two kinds of test substances may be contained in the container, for example, the test substances include a reagent and a magnetic bead adsorbate, and step S150 only draws out the unreacted test substances, and the magnetic bead adsorbate is retained in the container. In other embodiments, the process may be a draw (draw a portion of the test solution in the container), an evacuation (evacuate the container), an injection of reagents, an injection of cleaning fluids, and the like.
In one embodiment, the automated analyzer removes the test substance from the container and then performs a substance injection process on the container. The substance injected into the container may be a cleaning solution, a reagent, a sample, or the like. The second measurement value is obtained after the test substance is removed from the container and before the substance injection treatment. In this embodiment, step S150 is followed by a step of obtaining a third detection value according to the light intensity received by the light sensor at this time. And judging whether the difference value between the third detection value and the second detection value is larger than the error range, if so, judging that the material injection treatment is normal, otherwise, judging that the material injection treatment is abnormal.
It is understood that since the transmittance of the entire container changes after the test substance in the container is processed, if the difference between the third detection value and the second detection value is too small, it is determined that the substance injection process is abnormal. The error range is a range within which it is determined that the test substance in the container has not changed. The absolute value of the error range is smaller than that of the liquid adding range.
And S160, judging whether the difference value of the second detection value and the first detection value is larger than an error range.
If so, judging that the container processing is normal, otherwise, judging that the container processing is abnormal. Steps S130 to S160 belong to the in-operation detection phase P2.
According to the detection method for the container state in the working process of the automatic analysis device, the optical sensor is used for carrying out non-contact detection on the container state, so that the problem that a sample is possibly polluted in the detection process is solved.
The detection method combines the advantages of segmented detection and dynamic detection, and overcomes the respective disadvantages of the two types of detection. The simple segmentation detection needs to measure a large amount of empirical data in advance and then perform segmentation threshold setting on the basis of the empirical data, and the main disadvantage is poor compatibility. For example, the difference in transmittance due to the difference in surface smoothness between cuvettes (a clean cuvette has a high transmittance, and after a cuvette containing a sample such as plasma is drained, the wall of the cuvette remains and the transmittance is affected), or the difference in transparency of a liquid contained in the cuvette (such as serum, pure water, magnetic beads, etc.), is prone to cause erroneous judgment. Pure dynamic detection can only detect the dynamically changed state, and cannot distinguish and detect multiple states with certain certainty. If the change from the presence of a cup to the absence of a cup or the change from the presence of a liquid to the absence of a liquid can be detected by simple dynamic detection, the distinction degree of the presence of a cup, the absence of a cup, the presence of a liquid and the absence of a liquid is insufficient.
By adopting a mode of combining sectional detection and dynamic detection, firstly establishing a three-section model of no-shielding/empty container shielding/full shielding through a modeling stage, thereby detecting various states including normal container loading, abnormal container loading, normal container processing and abnormal container processing; the first detection value is obtained according to the container containing the experimental substance in the detection stage, whether the subsequent container is normal or not is judged according to the comparison with the first detection value, and the dynamic detection means can overcome the error caused by the change of the characteristics of the experimental substance in the container.
In one embodiment, the detection phase P2 further comprises the step of verifying the authenticity of the light sensor:
the automatic analysis device rotates the turntable to align the gap between the containers with the position of the optical sensor, and a fourth detection value is obtained according to the light intensity received by the optical sensor at the moment.
A stopper is provided, and the stopper may be made of the same material as the stopper when the second threshold is implemented in step S110. The turntable rotates to the position where the gap is aligned with the separation blade between the containers, and a fifth detection value is obtained according to the light intensity received by the light sensor at the moment.
And if the fourth detection value is in the redundant range of the first threshold value and the fifth detection value is in the redundant range of the second threshold value, judging that the optical sensor is normal, otherwise, judging that the optical sensor is abnormal, and stopping the detection in the detection stage P2 in work.
It will be appreciated that the fourth detection value should approach the first threshold value and the fifth detection value should approach the second threshold value, and if this condition is not met, indicating that the light sensor is abnormal, the test is stopped.
In one embodiment, the step S110 of obtaining the first threshold of the light intensity received by the light sensor in the non-shielding state is to control the turntable to rotate, obtain the light intensities at a plurality of different gaps, and obtain the first threshold after averaging.
The method for detecting the state of the container during the operation of the automatic analyzer is further described below with reference to an embodiment. In this embodiment, the light sensor is a reflective optical coupler, and the container is a reaction cup.
The modeling phase P1 includes the following steps:
the automatic analysis device controls the rotary disc to rotate, ten optical coupler voltage values Va 1-Va 10 at gaps, ten optical coupler voltage values Vb 1-Vb 10 at white baffles and ten optical coupler voltage values Vc 1-Vc 10 at reaction cups are recorded respectively, and Va0 ═ 10 (Va1+ Va2+. + Va10)/10, Vb0 ═ 10 (Vb1+ Vb2+. + Vb10)/10 and Vc0 ═ 10 (Vc1+ Vc2+. + Vc10)/10 are obtained through calculation. Setting a scale threshold value delta V0 as (Vb0-Va0)/N, wherein the scale threshold value is used as a numerical basis for the redundancy correction, the liquid adding range, the error range and the like in the detection stage P2 in the subsequent work, and is directly applied or used by taking the multiple/fraction of the scale threshold value; the scale factor N is an empirical value and is a natural number greater than 2 and smaller than 100. In this example, N is 16. The values of Va0, Vb0, Vc0, Δ V0 are recorded and stored.
In this embodiment, the in-operation detection stage P2 is a cleaning process, and in other embodiments, may be other operations of the automatic analyzer, such as adding reagents to the sample container. In the automatic analyzer, after the sample undergoes an immune reaction, the reaction product needs to be washed many times to remove free substances. In the process of cleaning free substances, the change of the liquid level needs to be monitored in real time, so that the cleaning effect is judged. The in-operation detection phase P2 of the present embodiment includes the following steps:
verifying the reliability of the optical sensor: the turntable moves to the position where the gap is aligned with the optocoupler, and the voltage Va' of the photoelectric switch at the moment is detected; setting a baffle plate, moving the turntable to the position where the baffle plate is aligned with the optocoupler, and detecting the voltage value Vb' of the optocoupler at the moment; and if (Va 0-delta V0) < Va '< (Va0+ delta V0) and (Vb 0-delta V0) < Vb' < (Vb0+ delta V0), judging that the optical coupler is normal. Wherein, Va 'is the fourth detection value, (Va 0-DeltaV 0) < Va' < (Va0+ DeltaV 0), that is, the fourth detection value is in the redundancy range of the first threshold value; vb 'is the fifth detection value, (Vb0- Δ V0) < Vb' < (Vb0 +. DELTA.v 0), i.e., the fifth detection value is within a redundant range of the second threshold value.
Primary cleaning: and (3) moving the turntable to the position of the reaction cup aligning with the optocoupler, detecting the voltage value Vc ' of the optocoupler at the moment, if (Va0 +. DELTA.V 0) < Vc ' < (Vb 0-DELTA.V 0) and (Vc 0-DELTA.V 0) < Vc ' < (Vc0 +. DELTA.V 0), indicating that the reaction cup is normally loaded, otherwise, indicating that the reaction cup is failed to be loaded. Wherein, Vc ' is the first detection value, (Va0 +. DELTA.V 0) is the first threshold value after the redundancy correction, (Vb 0-DELTA.V 0) is the second threshold value after the redundancy correction, (Vc 0-DELTA.V 0) < Vc ' < (Vc0 +. DELTA.V 0) indicates that Vc ' is in the liquid adding range of the third threshold value. And (3) moving a cleaning needle of the automatic analysis device, extracting liquid (serum waste liquid in the embodiment) in the reaction cup, lifting the cleaning needle after evacuating the reaction cup, detecting the voltage value Vd 'of the optical coupler at the moment, and if the voltage value Vd' is | Vc '-Vd' | >. DELTA.V 0, judging that the liquid extraction is normal (the liquid extraction is successful), otherwise, judging that the liquid extraction is abnormal. Where Vd ' is the second detection value, | Vc ' -Vd ' | >. Δ V0 indicates that the difference between the second detection value and the first detection value is greater than the error range. And injecting a cleaning liquid into the reaction cup by the cleaning needle, detecting the voltage value Ve ' of the optical coupler at the moment, judging that the cleaning liquid is injected normally (successfully) if | Vd ' -Ve ' | > [ Delta ] V0, and otherwise, indicating that the cleaning liquid is injected abnormally. Where Ve ' is the third detection value, | Vd ' -Ve ' | >. Δ V0 indicates that the difference between the third detection value and the second detection value is greater than the error range.
Secondary cleaning: and (3) moving the turntable to the position of the reaction cup aligned with the optocoupler, detecting the voltage value Vc 'of the optocoupler at the moment, if (Va0 +. DELTA.V 0) < Vc' < (Vb 0-DELTA.V 0), indicating that the reaction cup is loaded normally, otherwise, indicating that the reaction cup is loaded failure. And (3) moving the cleaning needle, extracting liquid in the reaction cup, evacuating the reaction cup, lifting the cleaning needle, detecting the voltage value Vd ' of the optical coupler at the moment, if the voltage value is | Vc ' -Vd ' > | DeltaV 0, judging that the liquid extraction is successful, otherwise, judging that the liquid extraction is abnormal. And injecting a cleaning liquid into the reaction cup by the cleaning needle, detecting the voltage value Ve of the optical coupler at the moment, judging that the injection of the cleaning liquid is successful if | Vd '-Ve' | > < delta > V0, and otherwise, indicating that the injection of the cleaning liquid is abnormal.
Because the turntable continuously rotates in the detection process, the state detection of a plurality of containers on the turntable can be realized by only configuring one optical sensor, the sensor cost is saved, and the system design is simplified.
The inventor also provides an automatic analysis device, which comprises a light sensor and a control system, wherein the control system is used for controlling the automatic analysis device to execute the detection method for the container state in the working process of the automatic analysis device according to any one of the embodiments.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. A method for detecting the state of a container in the working process of an automatic analysis device is characterized in that a mode of combining sectional detection and dynamic detection is adopted, the detection method comprises a modeling stage and a working detection stage after the modeling stage, and the modeling stage comprises the following steps:
acquiring a first threshold value of light intensity received by a light sensor in a corresponding non-shielding state, and acquiring a second threshold value of light intensity received by the light sensor in a corresponding full-shielding state;
obtaining a third threshold value according to the light intensity received by the light sensor in the state of being shielded by the empty container;
the in-service detection phase comprises:
the automatic analysis device moves the container containing the experimental substance to a position aligned with the optical sensor, and a first detection value is obtained according to the light intensity received by the optical sensor at the moment;
judging whether the first detection value simultaneously meets two conditions: the liquid filling range is between the first threshold value after the redundancy correction and the second threshold value after the redundancy correction and is within the liquid filling range of the third threshold value; if the two conditions are met simultaneously, judging that the container is normally loaded, otherwise, judging that the container is failed to be loaded;
after the automatic analysis device processes the experimental substance in the container, a second detection value is obtained according to the light intensity received by the light sensor at the moment;
judging whether the difference value between the second detection value and the first detection value is larger than the error range, if so, judging that the container processing is normal, otherwise, judging that the container processing is abnormal; the absolute value of the error range is smaller than the absolute value of the liquid adding range.
2. The method of claim 1, wherein the automated analyzer processes the test substance in the container, including the automated analyzer removes the test substance from the container.
3. The method of claim 2, wherein the automated analyzer processes the test substance in the container, including the automated analyzer performs a substance injection process on the container after removing the test substance from the container;
the second detection value is obtained after the experimental substance is removed from the container and before the substance injection treatment is carried out;
the in-service detection phase further comprises:
after the injection treatment of the substance, obtaining a third detection value according to the light intensity received by the optical sensor at the moment;
and judging whether the difference value of the third detection value and the second detection value is larger than the error range, if so, judging that the material injection treatment is normal, otherwise, judging that the material injection treatment is abnormal.
4. The detection method according to claim 3, wherein the substance injection treatment is performed by adding a cleaning solution to the container.
5. The inspection method according to claim 1, wherein the automatic analyzer comprises a carousel provided with a plurality of receiving locations for receiving the containers, each receiving location being adapted to receive one of the containers; when the containers are arranged on the turntable, a gap exists between every two adjacent containers;
the in-service detection phase further comprises:
the turntable rotates to a position where the gap is aligned with the optical sensor, and a fourth detection value is obtained according to the light intensity received by the optical sensor at the moment;
setting a blocking piece, rotating the turntable to a position where the optical sensor is aligned with the blocking piece, and obtaining a fifth detection value according to the light intensity received by the optical sensor at the moment;
and if the fourth detection value is in the redundancy range of the first threshold value and the fifth detection value is in the redundancy range of the second threshold value, judging that the optical sensor is normal.
6. The detection method according to any one of claims 1 to 5, wherein the light sensor is a reflective light coupler or a counter-reflective light coupler;
the modeling stage also comprises a step of subtracting the first threshold from the second threshold and dividing the subtracted result by a scale coefficient to obtain a scale threshold, wherein the scale coefficient is a natural number which is greater than 2 and smaller than 100;
the time between the first threshold value after the redundancy correction and the second threshold value after the redundancy correction is greater than the first threshold value plus scale threshold value and less than the second threshold value minus scale threshold value; the liquid adding range in the third threshold is larger than the third threshold minus the scale threshold and smaller than the third threshold plus the scale threshold; the absolute value of the error range is equal to the calibration threshold.
7. The detection method according to claim 6, wherein the scale factor is 16.
8. The method according to claim 1, wherein said obtaining of the second threshold corresponding to the intensity of light received by the light sensor in the fully shielded state is to align the light sensor with a white baffle, and the second threshold is obtained according to the intensity of light received by the light sensor.
9. The detection method according to claim 1, wherein the container is a translucent container.
10. An automatic analysis device comprising a control system, characterized in that said control system is adapted to perform a method for detecting the condition of a container during operation of an automatic analysis device according to any one of claims 1 to 9.
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| CN113125786B (en) * | 2021-04-28 | 2024-03-19 | 深圳市卓润生物科技有限公司 | Reaction cup state detection method and system |
| CN113589395A (en) * | 2021-07-15 | 2021-11-02 | 桂林优利特医疗电子有限公司 | Multichannel pipeline reagent alarm detection method and device in blood analyzer |
| CN114047342A (en) * | 2021-11-25 | 2022-02-15 | 精匠诊断技术(江苏)有限公司 | In-vitro analysis instrument and leakage detection method for in-vitro analysis instrument |
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