CN109554357A - One kind containing A138X1And L195X2The plant EPSPS mutant and its encoding gene of mutation and application - Google Patents

Abstract

The invention discloses one kind to contain A38X₁And L195X₂The plant EPSPS mutant and its encoding gene of mutation and application, are related to gene engineering technology field.Plant EPSPS mutant disclosed by the invention, compared with Escherichia coli EPSPS, the amino acid sequence of the plant EPSPS mutant corresponds to the 138th of Escherichia coli EPSPS with mutation A38X₁And/or the 195th have mutation L195X₂, wherein X₁=C, D, E, I, L, M, N or S, X₂=A, C, E, F, G, H, K, M, N, Q, R, S, T, V or Y.Any one site mutation or two site simultaneous mutations in two sites can assign or improve plant EPSPS mutant to the resistance of glyphosate.Convert the plant of the plant EPSPS mutant or recombinant bacterium normal growth under the conditions of can be existing for the glyphosate.

Description

One kind containing A138X1And L195X2The plant EPSPS mutant and its encoding gene of mutation And application

Technical field

The present invention relates to gene engineering technology fields, contain A138X in particular to one kind₁And L195X₂The plant of mutation EPSPS mutant and its encoding gene and application.

Background technique

Glyphosate is one of the most commonly used herbicide used in the world at present, has been used so far nearly 40 years.Grass Sweet phosphine inhibits the activity of 5- enolpyruvylshikimate -3- phosphate synthase (EPSPS).EPSPS is catalyzed PEP in shikimic acid pathway EPSP, final synthetic aromatic amino acid tryptophan, phenylalanine and tyrosine are synthesized with shikimate-3-phosphate. Glyphosate leads to aromatic series Amino acid biosynthesis block, and then influences plant normal growth, lethal eventually to die.

The main method for cultivating resistance glyphosate kind at present is to apply genetic engineering means by the resistance glyphosate from bacterium In channel genes crops, to cultivate transgenosis glyphosate resistant crops new varieties.Since 1996 start popularization, growing surface Product increases sharply, until 2015, global resistance glyphosate genetically modified crops cultivated area accounts for genetically modified crops up to 1.5 hundred million hectares The 83% of total cultivated area brings huge interests to agricultural production and environment.

But agriculturally most widely used Antiglyphosate gene is derived from Agrobacterium (Agrobacterium at present Tumefaciens) the CP4EPSPS of CP4 bacterial strain.Although successively having found the EPSPS base of many energy resistance glyphosates from microorganism Cause, but extensive use is not yet received in these genes in crops.These microorganism Antiglyphosate gene answering in crops With being to express these genes such as CP4EPSPS in crops with transgene method.The genetically modified crops generated therefrom Although there is sizable popularizing area, due to reverted gene tide, genetically modified crops global acceptance level still compared with It is low, even if it is several that transgenosis is also mainly limited to corn and soybean, cotton etc. in genetically modified crops cultivated area maximum America Crop.

Summary of the invention

The purpose of the present invention is to provide a kind of plant EPSPS mutant, (i.e. 5- enolpyruvylshikimate -3- phosphoric acid is synthesized Enzyme mutant), from plant, after mutated, it is provided with glyphosate resistance.

Another object of the present invention is to provide a kind of encoding gene, the above-mentioned plant EPSPS mutant of codified.

Another object of the present invention is to provide the carriers for containing above-mentioned encoding gene.

Another object of the present invention is to provide the cells for containing above-mentioned carrier.

Another object of the present invention is to provide the applications of above-mentioned plant EPSPS mutant.

The present invention is implemented as follows:

A kind of plant EPSPS mutant, compared with Escherichia coli EPSPS, the amino acid of the plant EPSPS mutant Sequence corresponds to the 138th of Escherichia coli EPSPS with mutation A138X₁And/or the 195th have mutation L195X₂, wherein X₁=C, D, E, I, L, M, N or S, X₂=A, C, E, F, G, H, K, M, N, Q, R, S, T, V or Y.

A kind of encoding gene encodes above-mentioned plant EPSPS mutant.

Carrier containing above-mentioned encoding gene.

Recombinant cell containing above-mentioned carrier.

Above-mentioned plant EPSPS mutant is cultivating the application in anti-glyphosate plants.

The invention has the following advantages:

Plant EPSPS mutant provided by the invention, compared with Escherichia coli EPSPS, the plant EPSPS mutant Amino acid sequence corresponds to the 138th of Escherichia coli EPSPS with mutation A138X₁And/or the 195th have mutation L195X₂, wherein X₁=C, D, E, I, L, M, N or S, X₂=A, C, E, F, G, H, K, M, N, Q, R, S, T, V or Y.Two sites In any one site mutation or various plants EPSPS mutant can be improved to glyphosate in protrusion simultaneously two sites Resistance, while keeping the biological enzyme activity of itself.Convert plant or the recombination of plant EPSPS mutant provided by the invention Normal growth under the conditions of bacterium can be existing for the glyphosate, the plant EPSPS mutant are applied not only to genetically modified crops cultivation, It can also be applied to cultivate resistance glyphosate non-transgenic plant such as rice, tobacco, soybean, corn, wheat, cotton and sorghum etc., It has broad application prospects.

Detailed description of the invention

In order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment attached Figure is briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not construed as pair The restriction of range for those of ordinary skill in the art without creative efforts, can also be according to this A little attached drawings obtain other relevant attached drawings.

Fig. 1 be the embodiment of the present invention 1 provide rice EPSP S mutant OsC, OsD, OsE, OsI, OsL, OsM, OsN, The amino acid sequence part comparison result of OsS, wild rice EPSPS and Escherichia coli EPSPS three；

Fig. 2 be the embodiment of the present invention 2 provide rice wild type EPSPS OsEPSPS_WT, rice EPSP S mutant OA, OAC, OAS, OASC, soybean wild type EPSPS GmEPSPS_WT, soy bean EPSPS mutant GA, GAC, GAS, GASN, small wheat fields The amino acid sequence part of raw type EPSPS TaEPSPS_WT, wheat EPSPS mutant TAS, TASC and Escherichia coli EPSPS Comparison result；

Fig. 3 be the embodiment of the present invention 3 provide rice wild type EPSPS OsEPSPS_WT, rice EPSP S mutant RA, RC, RE, RF, RG, RH, RK, RM, RN, RP, RQ, RR, RS, RT, RV, RY, soybean wild type EPSPS GmEPSPS_WT, soybean EPSPS mutant SP, SQ, wheat wild type EPSPS TaEPSPS_WT, wheat EPSPS mutant WA, WG, WM, WP, WQ, WS And the amino acid sequence part comparison result of Escherichia coli EPSPS；

Fig. 4 is soy bean EPSPS mutant GA, GAM, Wild-type soy EPSPS and the large intestine that the embodiment of the present invention 4 provides The amino acid sequence part comparison result of bacillus EPSPS；

Fig. 5 is the structural schematic diagram for the pADV5 carrier that experimental example 1 of the present invention provides；

Fig. 6 be the conversion embodiment 1 that provides of experimental example 1 of the present invention provide rice EPSP S mutant OsC, OsD, OsE, The Escherichia coli of OsI, OsL, OsM, OsN, OsS and wild rice EPSPS are on the culture medium of the glyphosate containing various concentration Growth result；

Fig. 7 is rice EPSP S mutant OAC, OASC that the conversion embodiment 2 that provides of experimental example 2 of the present invention provides and right According to growth result of the Escherichia coli on the culture medium of the glyphosate containing various concentration of OA, OAS；

Fig. 8 is soy bean EPSPS mutant GAC, GASN that the conversion embodiment 2 that provides of experimental example 2 of the present invention provides and right According to growth result of the Escherichia coli on the culture medium of the glyphosate containing various concentration of GA, GAS；

Fig. 9 is the wheat EPSPS mutant TASC that the conversion embodiment 2 that experimental example 2 of the present invention provides provides and control TAS Growth result of the Escherichia coli on the culture medium of the glyphosate containing various concentration；

Figure 10 be the conversion embodiment 3 that provides of experimental example 3 of the present invention provide rice EPSP S mutant RA, RC, RE, RF, The Escherichia coli of RG, RH, RK, RM, RN, RQ, RR, RS, RT, RV, RY and rice wild type EPSPS are in glyphosate containing various concentration Culture medium on growth result；

Figure 11 be the conversion embodiment 3 that provides of experimental example 4 of the present invention provide wheat EPSPS mutant WA, WP, WQ, WG, Growth result of the Escherichia coli of WS, WM and wheat wild type EPSPS on the culture medium of the glyphosate containing various concentration；

Figure 12 is soy bean EPSPS mutant SP, SQ and soybean that the conversion embodiment 3 that experimental example 4 of the present invention provides provides Growth result of the Escherichia coli of wild type EPSPS on the culture medium of the glyphosate containing various concentration；

Figure 13 is the soy bean EPSPS mutant GAM that the conversion embodiment 4 that experimental example 5 of the present invention provides provides and control GA Growth result of the Escherichia coli on the culture medium of the glyphosate containing various concentration.

Specific embodiment

It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, according to normal conditions or manufacturer builds The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase Product.

A138X is contained to the embodiment of the present invention below₁And L195X₂The plant EPSPS mutant and its encoding gene of mutation It is specifically described with application.

On the one hand, plant EPSPS mutant provided by the invention, compared with Escherichia coli EPSPS, the plant The amino acid sequence of EPSPS mutant corresponds to the 138th of Escherichia coli EPSPS with mutation A138X₁And/or the 195th With mutation L195X₂, wherein X₁=C, D, E, I, L, M, N or S, X₂=A, C, E, F, G, H, K, M, N, Q, R, S, T, V or Y.

That is by the ammonia of the amino acid sequence of plant EPSPS mutant provided by the invention and Escherichia coli EPSPS Base acid sequence is compared, and the amino acid sequence of the plant EPSPS mutant corresponds to the 138th of Escherichia coli EPSPS Site sports C, D, E, I, L, M, N by amino acid residue A (amino acid residue of the wild-type plant EPSPS in the site is A) Or S, or the 195th site corresponding to Escherichia coli EPSPS is by amino acid residue L (i.e. EPSPS pairs of wild-type plant Should be L in the amino acid residue in the site) sport A, C, E, F, G, H, K, M, N, Q, R, S, T, V or Y.

Certainly, plant EPSPS mutant provided by the invention can also have the various mutations in above-mentioned two site simultaneously.

In other words, plant EPSPS mutant provided by the invention is obtained by mutation as follows:

(1) wild-type plant EPSPS and Escherichia coli EPSPS is subjected to amino acid alignment, by wild-type plant EPSPS sports C, D, E, I, L, M, N or S corresponding to the 138th amino acid residue A of Escherichia coli EPSPS and obtains this hair The plant EPSPS mutant with glyphosate resistance of bright offer；

(2) alternatively, wild-type plant EPSPS and Escherichia coli EPSPS is carried out amino acid alignment, wild type is planted Object EPSPS corresponding to Escherichia coli EPSPS the 195th amino acid residue L sport A, C, E, F, G, H, K, M, N, Q, R, S, T, V or Y obtain the plant EPSPS mutant provided by the invention with glyphosate resistance；

(3) or, wild-type plant EPSPS and Escherichia coli EPSPS is subjected to amino acid alignment, by wild type Plant EPSPS sports C, D, E, I, L, M, N or S corresponding to the 138th amino acid residue A of Escherichia coli EPSPS and will Wild-type plant EPSPS corresponding to Escherichia coli EPSPS the 195th amino acid residue L sport A, C, E, F, G, H, K, M, N, Q, R, S, T, V or Y obtain the plant EPSPS mutant provided by the invention with glyphosate resistance；

It should be noted that wild-type plant EPSPS corresponds to Escherichia coli EPSPS the 138th and the 195th mutation Position, this mutated site are different due to different Species origins in position specific in wild-type plant EPSPS sequence.

Further, in some embodiments of the present invention, the amino acid sequence of the plant EPSPS mutant is corresponding The 96th in Escherichia coli EPSPS also also has mutation P101S with mutation G96A and/or the 101st.

That is by the ammonia of the amino acid sequence of plant EPSPS mutant provided by the invention and Escherichia coli EPSPS Base acid sequence is compared, and the amino acid sequence of the plant EPSPS mutant corresponds to 96 sites of Escherichia coli EPSPS S is sported by amino acid residue P by the site that amino acid residue G sports A and/or the 101st.

Further, in some embodiments of the present invention, the plant EPSPS mutant is from wheat, rice, big Wheat, oat, corn, sorghum, millet, buckwheat, maiden cane, mung bean, semen viciae fabae, pea, hyacinth bean, sweet potato, potato, cotton, soybean, oil Dish, sesame, peanut, sunflower, radish, carrot, turnip, beet, Chinese cabbage, leaf mustard, wild cabbage, cauliflower, cabbage mustard, cucumber, west It is cucurbit, pumpkin, wax gourd, balsam pear, sponge gourd, snake melon, watermelon, muskmelon, tomato, eggplant, capsicum, Kidney bean, cowpea, green soy bean, leek, big Green onion, leek, spinach, celery, three-coloured amaranth, lettuce, crowndaisy chrysanthemum, day lily, grape, strawberry, beet, sugarcane, tobacco, clover, is herded onion Any one plant in grass, turfgrass, tea and cassava.

Plant EPSPS mutant provided by the invention is by from rice, tobacco, soybean, corn, wheat, cotton, rape It is obtained after above-mentioned mutation with the wild type EPSPS of the plants such as sorghum, remains plant origin characteristic.It can will encode the plant The encoding gene of EPSPS mutant is applied to cultivate the variety of crops of resistance glyphosate, such as transgenosis can be used or gene is repaired The method of decorations, which is realized, cultivates purpose.The mode for comparing the gene of existing resistance glyphosate of the conversion from microorganism, directly with plant The Antiglyphosate gene in object source converts or carries out gene editing as template, has more reliable biological safety, favorably In the promotion and application of resistance glyphosate new varieties, public's acceptance level is improved.

Further, in some embodiments of the present invention, the amino acid sequence of Escherichia coli EPSPS such as SEQ ID Shown in NO.47.

Further, in some embodiments of the present invention, the amino sequence of EPSPS mutant is in following sequence Any one:

SEQ ID NO.49、SEQ ID NO.50、SEQ ID NO.51、SEQ ID NO.52、SEQ ID NO.53、SEQ ID NO.54、SEQ ID NO.55、SEQ ID NO.56、SEQ ID NO.58、SEQ ID NO.60、SEQ ID NO.63、SEQ ID NO.65、SEQ ID NO.68、SEQ ID NO.69、SEQ ID NO.70、SEQ ID NO.71、SEQ ID NO.72、SEQ ID NO.73、SEQ ID NO.74、SEQ ID NO.75、SEQ ID NO.76、SEQ ID NO.77、SEQ ID NO.79、SEQ ID NO.80、SEQ ID NO.81、SEQ ID NO.82、SEQ ID NO.83、SEQ ID NO.84、SEQ ID NO.85、SEQ ID NO.86, SEQ ID NO.87, SEQ ID NO.89, SEQ ID NO.90, SEQ ID NO.92 and SEQ ID NO.93.

Wherein, SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53、SEQ ID NO.54、SEQ ID NO.55、SEQ ID NO.56、SEQ ID NO.58、SEQ ID NO.60、SEQ ID NO.68、SEQ ID NO.69、SEQ ID NO.70、SEQ ID NO.71、SEQ ID NO.72、SEQ ID NO.73、SEQ ID NO.74、SEQ ID NO.75、SEQ ID NO.76、SEQ ID NO.77、SEQ ID NO.79、SEQ ID NO.80、SEQ ID NO.81, SEQ ID NO.82, EPSPS mutant shown in SEQ ID NO.83 and SEQ ID NO.84 are by coming from rice The wild rice EPSPS (SEQ ID NO.48) of (Oryza sativa) is obtained through after the mutation of glyphosate resistance mutational site It arrives, which includes: the 138th, the 195th corresponding to Escherichia coli shown in SEQ ID NO.47 And/or the 96th and the 101st.

Wherein, SEQ ID NO.63, SEQ ID NO.65, shown in SEQ ID NO.92 and SEQ ID NO.93 EPSPS mutant is by the Wild-type soy EPSPS (SEQ ID NO.61) from soybean (Glycine max (Linn.) Merr.) It is obtained through after the mutation of glyphosate resistance mutational site, which includes: corresponding to SEQ ID NO.47 Shown in the 138th, the 195th and/or the 96th and the 101st of Escherichia coli.

Wherein, SEQ ID NO.85, SEQ ID NO.86, SEQ ID NO.87, SEQ ID NO.89 and SEQ ID EPSPS mutant shown in NO.90 is by wild-type wheat EPSPS (the SEQ ID from wheat (Triticum aestivum) NO.66 it) is obtained through after the mutation of glyphosate resistance mutational site, which includes: corresponding to SEQ ID The 138th, the 195th and/or the 96th of Escherichia coli shown in NO.47 and the 101st.

On the other hand, the present invention also provides a kind of encoding genes, encode plant EPSPS mutant as described above.

Further, any one of the base sequence of these encoding genes in following sequence:

SEQ ID NO.2、SEQ ID NO.3、SEQ ID NO.4、SEQ ID NO.5、SEQ ID NO.6、SEQ ID NO.7、SEQ ID NO.8、SEQ ID NO.9、SEQ ID NO.11、SEQ ID NO.13、SEQ ID NO.16、SEQ ID NO.18、SEQ ID NO.21、SEQ ID NO.22、SEQ ID NO.23、SEQ ID NO.24、SEQ ID NO.25、SEQ ID NO.26、SEQ ID NO.27、SEQ ID NO.28、SEQ ID NO.29、SEQ ID NO.30、SEQ ID NO.32、SEQ ID NO.33、SEQ ID NO.34、SEQ ID NO.35、SEQ ID NO.36、SEQ ID NO.37、SEQ ID NO.38、SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.45 and SEQ ID NO.46.

Wherein,

SEQ ID NO.2 encodes rice EPSP S mutant shown in SEQ ID NO.49, is named as OsC in figure, table；

SEQ ID NO.3 encodes rice EPSP S mutant shown in SEQ ID NO.50, is named as OsD in figure, table；

SEQ ID NO.4 encodes rice EPSP S mutant shown in SEQ ID NO.51, is named as OsE in figure, table；

SEQ ID NO.5 encodes rice EPSP S mutant shown in SEQ ID NO.52, is named as OsI in figure, table；

SEQ ID NO.6 encodes rice EPSP S mutant shown in SEQ ID NO.53, is named as OsL in figure, table；

SEQ ID NO.7 encodes rice EPSP S mutant shown in SEQ ID NO.54, is named as OsM in figure, table；

SEQ ID NO.8 encodes rice EPSP S mutant shown in SEQ ID NO.55, is named as OsN in figure, table；

SEQ ID NO.9 encodes rice EPSP S mutant shown in SEQ ID NO.56, is named as OsS in figure, table；

SEQ ID NO.11 encodes rice EPSP S mutant shown in SEQ ID NO.58, is named as OAC in figure, table；

SEQ ID NO.13 encodes rice EPSP S mutant shown in SEQ ID NO.60, is named as in figure, table OASC；

SEQ ID NO.16 encodes soy bean EPSPS mutant shown in SEQ ID NO.63, is named as GAC in figure, table；

SEQ ID NO.18 encodes soy bean EPSPS mutant shown in SEQ ID NO.65, is named as in figure, table GASN；

SEQ ID NO.21 encodes rice EPSP S mutant shown in SEQ ID NO.68, is named as in figure, table TASC；

SEQ ID NO.22 encodes rice EPSP S mutant shown in SEQ ID NO.69, is named as RA in figure, table；

SEQ ID NO.23 encodes rice EPSP S mutant shown in SEQ ID NO.70, is named as RC in figure, table；

SEQ ID NO.24 encodes rice EPSP S mutant shown in SEQ ID NO.71, is named as RE in figure, table；

SEQ ID NO.25 encodes rice EPSP S mutant shown in SEQ ID NO.72, is named as RF in figure, table；

SEQ ID NO.26 encodes rice EPSP S mutant shown in SEQ ID NO.73, is named as RG in figure, table；

SEQ ID NO.27 encodes rice EPSP S mutant shown in SEQ ID NO.74, is named as RH in figure, table；

SEQ ID NO.28 encodes rice EPSP S mutant shown in SEQ ID NO.75, is named as RK in figure, table；

SEQ ID NO.29 encodes rice EPSP S mutant shown in SEQ ID NO.76, is named as RM in figure, table；

SEQ ID NO.30 encodes rice EPSP S mutant shown in SEQ ID NO.77, is named as RN in figure, table；

SEQ ID NO.32 encodes rice EPSP S mutant shown in SEQ ID NO.79, is named as RQ in figure, table；

SEQ ID NO.33 encodes rice EPSP S mutant shown in SEQ ID NO.80, is named as RR in figure, table；

SEQ ID NO.34 encodes rice EPSP S mutant shown in SEQ ID NO.81, is named as RS in figure, table；

SEQ ID NO.35 encodes rice EPSP S mutant shown in SEQ ID NO.82, is named as RT in figure, table；

SEQ ID NO.36 encodes rice EPSP S mutant shown in SEQ ID NO.83, is named as RV in figure, table；

SEQ ID NO.37 encodes rice EPSP S mutant shown in SEQ ID NO.84, is named as RY in figure, table；

SEQ ID NO.38 encodes wheat EPSPS mutant shown in SEQ ID NO.85, is named as WA in figure, table；

SEQ ID NO.39 encodes wheat EPSPS mutant shown in SEQ ID NO.86, is named as WG in figure, table；

SEQ ID NO.40 encodes wheat EPSPS mutant shown in SEQ ID NO.87, is named as WM in figure, table；

SEQ ID NO.42 encodes wheat EPSPS mutant shown in SEQ ID NO.89, is named as WQ in figure, table；

SEQ ID NO.43 encodes wheat EPSPS mutant shown in SEQ ID NO.90, is named as WS in figure, table；

SEQ ID NO.45 encodes soy bean EPSPS mutant shown in SEQ ID NO.92, is named as SQ in figure, table；

SEQ ID NO.46 encodes soy bean EPSPS mutant shown in SEQ ID NO.93, is named as GAM in figure, table.

It should be readily appreciated that arriving, to those skilled in the art, according to the degeneracy of codon, it is easy in above-mentioned coding The sequence basis of gene carries out the replacement of one or more nucleotide, corresponding derived sequence is obtained, so as to encode out the present invention The plant EPSPS mutant of offer.Therefore, replacing for one or more nucleotide is carried out in the sequence basis of above-mentioned encoding gene It changes, the derived sequence for being encoded out plant EPSPS mutant provided by the invention accordingly also belongs to protection model of the invention It encloses.

On the other hand, the present invention also provides the carriers for containing encoding gene as described above.

Further, in some embodiments of the present invention, carrier can be cloning vector, is also possible to expression and carries Body, further, in some embodiments of the present invention, expression vector can be prokaryotic expression carrier such as pADV5 carrier, It is also possible to carrier for expression of eukaryon.Further, in some embodiments of the present invention, carrier for expression of eukaryon is plant expression Carrier.

It is readily appreciated that, those skilled in the art are as needed, can choose suitable carrier, as the above-mentioned coding base of delivery The tool of cause, all belongs to the scope of protection of the present invention.

On the other hand, the present invention provides the recombinant bacteriums or recombinant cell that contain above-mentioned carrier.

Further, in some embodiments of the present invention, recombinant bacterium can be coccus, bacillus such as Escherichia coli or Person is spirillum；It is also possible to from oxygen bacterium either heterotrophic bacteria.

Further, in some embodiments of the present invention, it is thin can be also possible to eukaryon with prokaryotic cell for recombinant cell Born of the same parents；Further, in some embodiments of the present invention, eukaryocyte can be zooblast, be also possible to plant cell； Further, in some embodiments of the present invention, plant cell can be dicotyledonous plant cells, be also possible to unifacial leaf Plant cell.

It is readily appreciated that, those skilled in the art are as needed, can choose suitable bacterium or cell, as above-mentioned coding The host of gene, all belongs to the scope of protection of the present invention.

On the other hand, the present invention provides above-mentioned plant EPSPS mutant to cultivate the application in anti-glyphosate plants.

Further, in some embodiments of the present invention, above-mentioned application includes: that carrier is converted purpose plant, institute It states carrier and contains the encoding gene for encoding the plant EPSPS mutant.

For example, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID will be contained NO.6、SEQ ID NO.7、SEQ ID NO.8、SEQ ID NO.9、SEQ ID NO.11、SEQ ID NO.13、SEQ ID NO.16、SEQ ID NO.18、SEQ ID NO.21、SEQ ID NO.22、SEQ ID NO.23、SEQ ID NO.24、SEQ ID NO.25、SEQ ID NO.26、SEQ ID NO.27、SEQ ID NO.28、SEQ ID NO.29、SEQ ID NO.30、SEQ ID NO.32、SEQ ID NO.33、SEQ ID NO.34、SEQ ID NO.35、SEQ ID NO.36、SEQ ID NO.37、SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.45 or SEQ The carrier transformed cells such as Rice Callus of rice EPSP S mutant code gene shown in ID NO.46, culture make it It is differentiated to form complete rice plant, transgenosis glyphosate-resistant rice can be cultivated.Base is turned using the conversion of this kind of encoding gene Because rice plant not only has glyphosate resistance, and it does not introduce the foreign gene i.e. gene of different genera, and public affairs can be improved Many acceptance levels.

Further, in some embodiments of the present invention, above-mentioned application includes: the endogenous of modification purpose plant EPSPS gene makes it encode the plant EPSPS mutant.

For example, with SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7、SEQ ID NO.8、SEQ ID NO.9、SEQ ID NO.11、SEQ ID NO.13、SEQ ID NO.16、 SEQ ID NO.18、SEQ ID NO.21、SEQ ID NO.22、SEQ ID NO.23、SEQ ID NO.24、SEQ ID NO.25、SEQ ID NO.26、SEQ ID NO.27、SEQ ID NO.28、SEQ ID NO.29、SEQ ID NO.30、SEQ ID NO.32、SEQ ID NO.33、SEQ ID NO.34、SEQ ID NO.35、SEQ ID NO.36、SEQ ID NO.37、SEQ ID NO.38、SEQ ID NO.39、SEQ ID NO.40、SEQ ID NO.42、SEQ ID NO.43、SEQ ID NO.45、SEQ ID Some or all of rice EPSP S mutant code gene shown in NO.46 is template, to the endogenous EPSPS of rice genome Gene is modified, and then can cultivate non-transgenic rice.

Further, in some embodiments of the present invention, above-mentioned application include: to plant cell, tissue, individual or Group carries out mutagenesis, screening, it is made to encode the plant EPSPS mutant.

For example, being guidance with OsC mutant code gene shown in SEQ ID NO.2, mutagenesis is carried out to rice material, such as Chemistry, radioinduction etc., and then the rice of endogenous EPSPS gene mutation can be cultivated.Such as it can also be by zinc finger nucleic acid Enzyme cutting (ZFN, zinc-finger nucleases) technology, class activating transcription factor effector nuclease (TALEN, Transcription activator-like effector nucleases) genes such as technology or CRISPR/Cas9 technology volume The coded sequence of endogenous EPSPS gene is modified to the base sequence (such as SEQ ID NO.2) of the encoding gene by the technology of collecting, The albumen equally with rice Os C mutant provided by the invention (SEQ ID NO.49) is encoded out in obtained purpose plant, is made Obtaining finally formed purpose plant has glyphosate resistance.

Further, in some embodiments of the present invention, above-mentioned plant (can be regarded as needing the target of gene modification Plant) be wheat, rice, barley, oat, corn, sorghum, millet, buckwheat, maiden cane, mung bean, semen viciae fabae, pea, hyacinth bean, sweet potato, Potato, cotton, soybean, rape, sesame, peanut, sunflower, radish, carrot, turnip, beet, Chinese cabbage, leaf mustard, wild cabbage, Cauliflower, cabbage mustard, cucumber, cucurbita pepo, pumpkin, wax gourd, balsam pear, sponge gourd, snake melon, watermelon, muskmelon, tomato, eggplant, capsicum, dish Beans, cowpea, green soy bean, leek, shallot, onion, leek, spinach, celery, three-coloured amaranth, lettuce, crowndaisy chrysanthemum, day lily, grape, strawberry, sweet tea Any one in dish, sugarcane, tobacco, clover, herbage, turfgrass, tea and cassava.

It is readily appreciated that, those skilled in the art are as needed, can choose the variety of crops for needing to cultivate, as long as answering It is belonged to the scope of protection of the present invention with plant EPSPS mutant provided by the invention and/or its encoding gene.

It should be noted that it is that Clustal is online that protein sequence according to the present invention, which compares used alignments, It compares, station address are as follows: http://www.ebi.ac.uk/Tools/msa/clustalo/.Using other sequence alignments The result that result obtained by tool (such as DNAMAN, relative parameters setting is by default setting) and Clustal are compared online It is almost the same.

To sum up, EPSPS mutants provided by the invention such as SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51、SEQ ID NO.52、SEQ ID NO.53、SEQ ID NO.54、SEQ ID NO.55、SEQ ID NO.56、SEQ ID NO.58、SEQ ID NO.60、SEQ ID NO.63、SEQ ID NO.65、SEQ ID NO.68、SEQ ID NO.69、SEQ ID NO.70、SEQ ID NO.71、SEQ ID NO.72、SEQ ID NO.73、SEQ ID NO.74、SEQ ID NO.75、SEQ ID NO.76、SEQ ID NO.77、SEQ ID NO.79、SEQ ID NO.80、SEQ ID NO.81、SEQ ID NO.82、SEQ ID NO.83、SEQ ID NO.84、SEQ ID NO.85、SEQ ID NO.86、SEQ ID NO.87、SEQ ID NO.88、SEQ ID NO.89, SEQ ID NO.90, SEQ ID NO.91, SEQ ID NO.92 or SEQ ID NO.93, with Escherichia coli EPSPS It compares, the amino acid sequence of the plant EPSPS mutant corresponds to the 138th of Escherichia coli EPSPS (SEQ ID NO.47) With mutation A138X₁And/or the 195th have mutation L195X₂, wherein X₁=C, D, E, I, L, M, N or S, X₂=A, C, E, F, G, H, K, M, N, Q, R, S, T, V or Y.

Various plants can be improved in any one site mutation or two site simultaneous mutations in two sites EPSPS mutant keeps itself biological enzyme activity to the resistance of glyphosate.Convert plant provided by the invention The normal growth under the conditions of plant of EPSPS mutant or recombinant bacterium can be existing for the glyphosates, the plant EPSPS mutant It is applied not only to genetically modified crops and cultivates outer, can also be applied to cultivate resistance glyphosate non-transgenic plant such as rice, tobacco, big Beans, corn, wheat, cotton and sorghum etc., have broad application prospects.

Feature and performance of the invention are described in further detail with reference to embodiments.

Embodiment 1

Rice EPSP S mutant provided in this embodiment, is named as OsC, OsD, OsE, OsI, OsL, OsM, OsN and OsS, Its amino acid sequence is respectively such as SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, SEQ ID NO.54, SEQ ID NO.55, shown in SEQ ID NO.56.

Rice EPSP S mutant provided in this embodiment (is named as OsEPSPS_WT, amino by wild rice EPSPS Acid sequence is as shown in SEQ ID NO.48) corresponding to Escherichia coli EPSPS (amino acid sequence is as shown in SEQ ID NO.47) 138th (mutational site be located at wild rice EPSPS the 155th) by amino acid residue A sport C, D, E, I, L, M, N or S are obtained.

Rice EPSP S mutant OsC, OsD, OsE, OsI, OsL, OsM, OsN and OsS, wild rice EPSPS and big The amino acid alignment of enterobacteria EPSPS three is as shown in Figure 1, in figure: position indicated by arrow is mutational site, Ec- EPSPS_WT represents Escherichia coli EPSPS.

In addition, the present embodiment additionally provide coding rice EPSP S mutant OsC, OsD, OsE, OsI, OsL, OsM, OsN and The nucleic acid molecules (i.e. encoding gene) of OsS, base sequence such as SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, shown in SEQ ID NO.9.

Rice EPSP S mutant OsC, OsD, OsE, OsI, OsL, OsM, OsN and OsS provided in this embodiment and encode it Nucleic acid molecules can be obtained by chemically synthesized method.

Embodiment 2

Rice EPSP S mutant provided in this embodiment is named as OAC, OASC, amino acid sequence such as SEQ ID Shown in NO.58, SEQ ID NO.60；Soy bean EPSPS mutant provided in this embodiment is named as GAC, GASN, amino acid sequence Column are as shown in SEQ ID NO.63, SEQ ID NO.65；The wheat EPSPS mutant of offer is named as TASC, amino acid sequence Column are as shown in SEQ ID NO.68.

Rice EPSP S mutant OAC, OASC provided in this embodiment is by wild rice EPSPS (OsEPSPS_WT, ammonia Base acid sequence is as shown in SEQ ID NO.48) correspond to Escherichia coli EPSPS (amino acid sequence is as shown in SEQ ID NO.47) The 138th (mutational site be located at wild rice EPSPS the 155th) sport C and the 96th by amino acid residue A The 111st of wild rice EPSPS (site correspond to) by amino acid G sports A and the 101st, and (site is corresponding In the 116th of wild rice EPSPS) it S is sported by amino acid P obtains.

Soy bean EPSPS mutant GAC, GASN provided in this embodiment is by Wild-type soy EPSPS (GmEPSPS_WT, ammonia Base acid sequence is as shown in SEQ ID NO.61) correspond to Escherichia coli EPSPS (amino acid sequence is as shown in SEQ ID NO.47) The 138th (mutational site be located at Wild-type soy EPSPS the 153rd) C (GAC) or N are sported by amino acid residue A (GASN) and the 96th the 109th of Wild-type soy EPSPS (site correspond to) sports A and the 101st by amino acid G Position the 114th of Wild-type soy EPSPS (site correspond to) sports S by amino acid P and obtains.

Wheat EPSPS mutant TASC provided in this embodiment is by wild-type wheat EPSPS (TaEPSPS_WT, amino acid Sequence is as shown in SEQ ID NO.66) corresponding to Escherichia coli EPSPS (amino acid sequence is as shown in SEQ ID NO.47) the Sporting C and the 96th by amino acid residue A for 138 (mutational site be located at wild-type wheat EPSPS the 157th) (should Site corresponds to the 113rd of wild-type wheat EPSPS) sporting A and the 101st by amino acid G, (site corresponds to open country The 118th of raw type wheat EPSPS) it S is sported by amino acid P obtains.

The amino acid alignment of above-mentioned EPSPS mutant, corresponding wild type EPSPS and Escherichia coli EPSPS is such as Shown in Fig. 2, in figure: position indicated by arrow is mutational site, and EcEPSPS_WT represents Escherichia coli EPSPS.

Embodiment 3

Rice EPSP S mutant provided in this embodiment, be named as RA, RC, RE, RF, RG, RH, RK, RM, RN, RQ, RR, RS, RT, RV and RY, amino acid sequence is respectively such as SEQ ID NO.69, SEQ ID NO.70, SEQ ID NO.71, SEQ ID NO.72、SEQ ID NO.73、SEQ ID NO.74、SEQ ID NO.75、SEQ ID NO.76、SEQ ID NO.77、SEQ ID NO.79, SEQ ID NO.80, SEQ ID NO.81, SEQ ID NO.82, SEQ ID NO.83, shown in SEQ ID NO.84.

Rice EPSP S mutant provided in this embodiment (is named as OsEPSPS_WT, amino by wild rice EPSPS Acid sequence is as shown in SEQ ID NO.48) corresponding to Escherichia coli EPSPS (amino acid sequence is as shown in SEQ ID NO.47) 195th (mutational site be located at wild rice EPSPS the 214th) by amino acid residue L sport A, C, E, F, G, H, K, M, N, Q, S, T, V or Y are obtained.

Rice EPSP S mutant RA, RC, RE, RF, RG, RH, RK, RM, RN, RQ, RR, RS, RT, RV and RY, wild type water The amino acid alignment of rice EPSPS and Escherichia coli EPSPS three are as shown in figure 3, in figure: position indicated by arrow is Mutational site, Ec-EPSPS_WT represent Escherichia coli EPSPS.

The present embodiment additionally provides wheat EPSPS mutant, is named as WA, WG, WM, WQ and WS, amino acid sequence point Not as shown in SEQ ID NO.85, SEQ ID NO.86, SEQ ID NO.87, SEQ ID NO.89, SEQ ID NO.90.

Wheat EPSPS mutant provided in this embodiment (is named as TaEPSPS_WT, amino by wild-type wheat EPSPS Acid sequence is as shown in SEQ ID NO.66) corresponding to Escherichia coli EPSPS (amino acid sequence is as shown in SEQ ID NO.47) A, G, M, Q or S are sported by amino acid residue L 195th (mutational site be located at wild-type wheat EPSPS the 216th) It obtains.

Wheat EPSPS mutant WA, WG, WM, WQ and WS, wild-type wheat EPSPS and Escherichia coli EPSPS three Amino acid alignment is as shown in figure 3, in figure: position indicated by arrow is mutational site, and Ec-EPSPS_WT represents large intestine bar Bacterium EPSPS.

The present embodiment additionally provides soy bean EPSPS mutant, is named as SQ, amino acid sequence is respectively such as SEQ ID Shown in NO.92.

Soy bean EPSPS mutant provided in this embodiment (is named as GmEPSPS_WT, amino by Wild-type soy EPSPS Acid sequence is as shown in SEQ ID NO.61) corresponding to Escherichia coli EPSPS (amino acid sequence is as shown in SEQ ID NO.47) 195th (mutational site be located at Wild-type soy EPSPS the 212nd) sports Q by amino acid residue L and obtains.

Soy bean EPSPS mutant SQ, Wild-type soy EPSPS and the amino acid sequence of Escherichia coli EPSPS three ratio Pair as shown in figure 3, in figure: position indicated by arrow is mutational site, and Ec-EPSPS_WT represents Escherichia coli EPSPS.

In addition, the present embodiment additionally provide coding EPSPS mutant RA, RC, RE, RF, RG, RH, RK, RM, RN, RQ, RR, The nucleic acid molecules (i.e. encoding gene) of RS, RT, RV, RY, WA, WG, WM, WP, WQ, WS, SP and SQ, base sequence is respectively such as SEQ ID NO.22、SEQ ID NO.23、SEQ ID NO.24、SEQ ID NO.25、SEQ ID NO.26、SEQ ID NO.27、SEQ ID NO.28、SEQ ID NO.29、SEQ ID NO.30、SEQ ID NO.32、SEQ ID NO.33、SEQ ID NO.34、SEQ ID NO.35、SEQ ID NO.36、SEQ ID NO.37、SEQ ID NO.38、SEQ ID NO.39、SEQ ID NO.40, SEQ ID NO.42, SEQ ID NO.43, shown in SEQ ID NO.45.

EPSPS mutant RA, RC provided in this embodiment, RE, RF, RG, RH, RK, RM, RN, RQ, RR, RS, RT, RV, RY, WA, WG, WM, WQ, WS and SQ and the nucleic acid molecules for encoding it can be obtained by chemically synthesized method.

Embodiment 4

Soy bean EPSPS mutant provided in this embodiment is named as GAM, amino acid sequence such as SEQ ID NO.93 institute Show.

Soy bean EPSPS mutant GAM provided in this embodiment is by Wild-type soy EPSPS (GmEPSPS_WT, amino acid sequence Column as shown in SEQ ID NO.61) corresponding to Escherichia coli EPSPS (amino acid sequence is as shown in SEQ ID NO.47) the 195th Position (mutational site be located at Wild-type soy EPSPS the 212nd) sports M and the 96th (site by amino acid residue L The 109th corresponding to Wild-type soy EPSPS) it A is sported by amino acid residue G obtains.

The amino acid alignment of above-mentioned EPSPS mutant, corresponding wild type EPSPS and Escherichia coli EPSPS is such as Shown in Fig. 4, in figure: position indicated by arrow is mutational site, and EcEPSPS_WT represents Escherichia coli EPSPS.

Experimental example 1

Detect the grass of rice EPSP S mutant OsC, OsD, OsE, OsI, OsL, OsM, OsN and OsS that embodiment 1 provides Sweet phosphine resistance, the method is as follows:

It is prominent using chemically synthesized method composite coding rice EPSP S according to the sequence for the nucleic acid molecules that embodiment provides The encoding gene of variant OsC, OsD, OsE, OsI, OsL, OsM, OsN and OsS, both ends introduce restriction enzyme site (Pac1 and Sbf1), After digestion, it is connected under the action of ligase through identical digestion treated expression vector (such as pADV5 carrier, structure On as shown in Figure 5), the Escherichia coli that then conversion pair knocks out, after verified, picking positive colony is seeded to containing various concentration It is grown on the M9 culture medium of glyphosate, observes Escherichia coli Growth situation.

With containing wild rice EPSPS mutant, (for amino acid sequence as shown in SEQ ID NO.48, encoding gene is such as Shown in SEQ ID NO.1), OsF (the 138th amino acid A of A138F, rice EPSP S sport F), OsG (A138G), OsH (A138H)、OsK(A138K)、OsP(A138P)、OsQ(A138Q)、OsR(A138R)、OsS(A138S)、OsV(A138V)、OsW (A138W) and OsY (A138Y) is used as negative control, and OsT (A138T) is positive control.As a result as shown in Figure 6.

Wherein, M9 basal medium can refer to following method and prepare:

5 × M9 salting liquid: 6.78g Na is weighed₂HPO₄, 3.0g KH₂PO₄, 0.5g NaCl, 1.0g NH₄Cl adds ddH₂O To 200ml, autoclave sterilization processing；

20% glucose: glucose sugar 20g is weighed, 80ml ddH is added₂O dissolution is settled to 100ml, filtration sterilization processing；

1.0M MgSO₄: weigh 24.6g MgSO₄-7H₂O, with the ddH of 80ml₂O dissolution, is settled to 100ml, at sterilizing Reason；

1.0M CaCl₂: weigh 11.1g CaCl₂, with the ddH of 80ml₂O dissolution, is settled to 100ml, sterilization treatment；

With 200ml 5 × M9 salting liquid, 20% glucose of 20ml, 2ml 1.0M MgSO₄With 0.1ml 1.0M CaCl₂, Add ddH₂O complements to 1000ml, that is, is configured to 1000ml M9 basal medium.

Wherein, Escherichia coli used are to knock out Escherichia coli EPSPS knock-out bacterial strain (the large intestine bar after knocking out EPSPS gene Bacterium DH5 α, is named as EDE, and preparation method can refer to the Chinese invention patent application No. is 2016103256926).

It can be seen that according to the result of Fig. 6

On the culture medium of the glyphosate containing 0mM, conversion encoding wild type rice EPSP S OsEPSPS_WT, other control with And the knock-out bacterial strain of the encoding gene of rice EPSP S mutant OsC, OsD, OsE, OsI, OsL, OsM, OsN and OsS, except OsK and Outside OsW slow growth, equal energy normal growth shows the EPSPS encoded by OsC, OsD, OsE, OsI, OsL, OsM, OsN and OsS All there is normal EPSPS enzyme activity；

On the culture medium of the glyphosate containing 1mM, the Escherichia coli Growth for converting wild rice EPSPS and negative control is slow Or it cannot grow substantially, but the large intestine bar of rice transformation EPSPS mutant OsC, OsD, OsE, OsI, OsL, OsM, OsN and OsS Bacterium growth is substantially better than all negative controls, illustrates the anti-grass of single mutant containing OsC, OsD, OsE, OsI, OsL, OsM, OsN and OsS The ability of sweet phosphine is substantially better than wild type and negative control；

On the culture medium of glyphosate containing 2mM and 5mM glyphosate, the large intestine of rice transformation EPSPS wild type and negative control The sign that bacillus does not grow, on the culture medium of the glyphosate containing 5mM, positive control also fails to grow, in contrast, rice transformation The Escherichia coli of EPSPS mutant OsC, OsD, OsE, OsI, OsL, OsM, OsN and OsS can give birth on both culture mediums It is long, illustrate that OsC, OsD, OsE, OsI, OsL, OsM, OsN and OsS mutation can significantly improve glyphosate resistance.

Experimental example 2

The grass of the detection method of reference experiment example 1, rice EPSP S mutant OAC and OASC that verifying embodiment 2 provides is sweet Phosphine resistance.As a result as shown in Figure 7.

It can be seen that according to the result of Fig. 7

On the culture medium of the glyphosate containing 0mM and 20mM, conversion coding rice EPSP S OA, OAS and rice EPSP S are prominent The Escherichia coli of the encoding gene of variant OAC and OASC can grow, and on the culture medium of the glyphosate containing 50mM, convert OA's Escherichia coli cannot grow, and the Escherichia coli for converting OAS only have faint growth substantially, but convert the Escherichia coli of OAC and OASC There is obvious growth, illustrates that the EPSPS of the mutation containing A155 (138) C can be mutated in G111 (96) A and/or P116 (101) S and mention Glyphosate resistance is further significantly improved on the basis of the glyphosate resistance of confession.

Experimental example 2 also demonstrates the glyphosate resistance of soy bean EPSPS the mutant GAC and GASN of the offer of embodiment 2.As a result As shown in Figure 8.

It can be seen that according to the result of Fig. 8

On the culture medium of the glyphosate containing 0mM, conversion coding soy bean EPSPS GA, GAS and soy bean EPSPS mutant The Escherichia coli of GAC and GASN encoding gene can grow, and on the culture medium of the glyphosate containing 20mM, convert this four genes Escherichia coli have a growth, but the Escherichia coli Growth of untransformed mutants GAC and GASN encoding gene be substantially better than control GA and GAS, and on the culture medium of the glyphosate containing 50mM, the Escherichia coli of conversion GA and GAS cannot grow, but convert GAC and GASN Escherichia coli have obvious growth, illustrate containing A153 (138) C mutation EPSPS can G109 (96) A be mutated provide grass Glyphosate resistance is further significantly improved on sweet phosphine underlying resistance, similarly the EPSPS of the mutation containing A153 (138) N can be Glyphosate resistance is further significantly improved on the basis of the glyphosate resistance that G109 (96) A and P114 (101) S mutation provide.

Experimental example 2 also demonstrates the glyphosate resistance of the wheat EPSPS mutant TASC of the offer of embodiment 2.As a result such as Fig. 9 It is shown.

It can be seen that according to the result of Fig. 9

On the culture medium of the glyphosate containing 0mM and 20mM, encoding wheat EPSPS TAS (control) and wheat are converted The Escherichia coli of EPSPS mutant TASC encoding gene can grow, and on the culture medium of the glyphosate containing 50mM, convert TAS Escherichia coli cannot grow, but convert TASC Escherichia coli have obvious growth, illustrate containing A157 (138) C be mutated wheat It is sweet that EPSPS can further significantly improve grass on the basis of G113 (96) A and P118 (101) S are mutated the glyphosate resistance provided Phosphine resistance.

Experimental example 3

Detect embodiment 3 provide rice EPSP S mutant RA, RC, RE, RF, RG, RH, RK, RM, RN, RQ, RR, RS, The glyphosate resistance of RT, RV and RY, method is as follows referring to experimental example 1.

With containing wild rice EPSPS mutant OsEPSPS_WT, (amino acid sequence is compiled as shown in SEQ ID NO.48 Code gene as shown in SEQ ID NO.1), RD (the amino acid L of the 214th (195) position of L195D, rice EPSP S sports D), RI (L195I) and RW (L195W) is used as negative control, and RP (L195P) is positive control.The results are shown in Figure 10.

It can be seen that according to the result of Figure 10

On the culture medium of the glyphosate containing 0mM, conversion encoding wild type rice EPSP S OsEPSPS_WT, other control with And the encoding gene of rice EPSP S mutant RA, RC, RE, RF, RG, RH, RK, RM, RN, RQ, RR, RS, RT, RV and RY strikes Except bacterial strain, in addition to negative control RD is not grown, can normal growth, show by RA, RC, RE, RF, RG, RH, RK, RM, RN, RQ, The EPSPS of RR, RS, RT, RV and RY coding has normal EPSPS enzyme activity；

On the culture medium of the glyphosate containing 1mM, the Escherichia coli Growth for converting wild rice EPSPS and negative control is slow Or it cannot grow substantially, but rice transformation EPSPS mutant RA, RC, RE, RF, RG, RH, RK, RM, RN, RQ, RR, RS, RT, RV Be substantially better than all negative controls and wild type with the Escherichia coli Growth of RY, illustrate containing RA, RC, RE, RF, RG, RH, RK, RM, The ability of the single mutant resistance glyphosate of RN, RQ, RR, RS, RT, RV and RY is substantially better than wild type and negative control；

On the culture medium of the glyphosate of glyphosate containing 2mM, the Escherichia coli of rice transformation EPSPS wild type and negative control do not have There is a sign of growth, and the Escherichia coli Growth energy normal growth of rice transformation EPSPS mutant RA, RE, RG, RH and RQ, On the more culture medium of glyphosate high concentration (5mM), the Escherichia coli of rice transformation EPSPS mutant RA, RE, RG, RH and RQ are all There are also obvious growths.

These results illustrate the single mutant of RA, RC, RE, RF, RG, RH, RK, RM, RN, RQ, RR, RS, RT, RV and RY all Ability with resistance glyphosate, and mutant RA, RE, RG, RH and RQ resistance glyphosate ability is stronger.

Experimental example 4

The glyphosate resistance of wheat EPSPS mutant WA, WG, WM, WQ and WS that embodiment 4 provides are detected, method is as follows Referring to experimental example 1.

With containing wild-type wheat EPSPS mutant TaEPSPS_WT, (amino acid sequence is compiled as shown in SEQ ID NO.66 Code gene is as shown in SEQ ID NO.19) it is used as negative control, WP (the amino acid L of the 216th (195) position of L195P, wheat EPSPS Sport P) it is positive control.As a result as shown in figure 11.

It can be seen that according to the result of Figure 11

On the culture medium of the glyphosate containing 0mM, conversion encoding wild type wheat EPSPS (W_WT), WP and wheat EPSPS The knock-out bacterial strain of the encoding gene of mutant WA, WG, WM, WQ and WS energy normal growth, shows to be compiled by WA, WG, WM, WQ and WS The EPSPS of code has normal EPSPS enzyme activity；

On the culture medium of the glyphosate containing 1mM, the Escherichia coli Growth for converting wild-type wheat EPSPS (W_WT) is slow, But the Escherichia coli Growth of transformed wheat EPSPS mutant WA, WG, WM, WQ and WS are substantially better than W_WT, illustrate containing WA, WG, The obvious wild type of ability of the single mutant resistance glyphosate of WM, WQ and WS；

On the culture medium of the glyphosate of glyphosate containing 2mM, the Escherichia coli of transformed wheat EPSPS wild type control are not given birth to Long sign, and the Escherichia coli of transformed wheat EPSPS mutant WA, WG, WM, WQ and WS have an obvious growth, and transformed wheat The Escherichia coli Growth of EPSPS mutant WA, WG and WS are normal, and are substantially better than positive control WP.

These results illustrate that the single mutant of WA, WG, WM, WQ and WS all have an ability of resistance glyphosate, and mutant WA, WG and WS resistance glyphosate ability is stronger.

This experimental example also has detected the glyphosate resistance of the soy bean EPSPS mutant SQ of the offer of embodiment 4, and method is joined as follows According to experimental example 1.

With containing Wild-type soy EPSPS mutant GmEPSPS_WT, (amino acid sequence is compiled as shown in SEQ ID NO.61 Code gene is as shown in SEQ ID NO.14) it is used as negative control, SP (L195P, the amino acid L of the 212nd (195) position of soy bean EPSPS Sport P) it is positive control.As a result as shown in figure 12.

It can be seen that according to the result of Figure 12

On the culture medium of the glyphosate containing 0mM, conversion encoding wild type soy bean EPSPS (S_WT), SP and soy bean EPSPS The knock-out bacterial strain of the encoding gene of mutant SQ energy normal growth, shows that the EPSPS encoded by SQ has normal EPSPS enzyme Vigor；

On the culture medium of the glyphosate containing 1mM, the Escherichia coli Growth for converting Wild-type soy EPSPS (S_WT) is faint, But the Escherichia coli Growth of soybean transformation EPSPS mutant SQ and positive control SP are substantially better than S_WT, illustrate the single mutation containing SQ The obvious wild type of the ability of body resistance glyphosate；

On the culture medium of the glyphosate of glyphosate containing 2mM, the Escherichia coli of soybean transformation EPSPS wild type control are not given birth to Long sign, and the Escherichia coli of soybean transformation EPSPS mutant SQ have obvious growth.

These results illustrate that the single mutant of SQ all has the ability of resistance glyphosate.

Experimental example 5

The glyphosate of the detection method of reference experiment example 1, the soy bean EPSPS mutant GAM that verifying embodiment 4 provides is anti- Property.As a result as shown in figure 13.

It can be seen that according to the result of Figure 13

Containing 0mM, the large intestine bar of the encoding gene of conversion coding soy bean EPSPS GA and soy bean EPSPS mutant GAM Bacterium can grow, and on the culture medium of the glyphosate containing 20mM, GA and GAM have some growths, but the growth of GAM becomes apparent from, On the culture medium of the glyphosate containing 50mM, the Escherichia coli for converting GA cannot grow, and the Escherichia coli for converting GAM have obvious growth, Illustrate that the EPSPS of the mutation containing L212 (195) M can be further bright on the basis of G109 (96) A is mutated the glyphosate resistance provided It is aobvious to improve glyphosate resistance.

Experimental example 6

Detect RA (SEQ ID NO.69), RK (SEQ ID NO.75) and RQ (SEQ ID NO.79) that embodiment 3 provides The enzyme kinetics parameter of rice EPSP S mutant and the enzyme kinetics parameter when there is glyphosate, the method is as follows:

Vector construction:

With the upstream primer that has restriction enzyme site NdeI and the downstream primer PCR amplification target gene for having restriction enzyme site XhoI, Purification and recovery.NdeI/XhoI double digestion target fragment and prokaryotic expression carrier pET32a, isolated fragment, with T4 DNA are used respectively Ligase connection converts DE3 competent cell, and conversion condition is ice bath 30min, then heat shock 90s, ice bath 3min are added 700 μ l LB culture medium, 37 degree slowly shake 1 hour after be coated on LBA solid plate, 37 degree inversions be incubated overnight after, picking monoclonal exists It is cultivated in LBA culture medium.Sequence verification clone.

6His protein purification:

(1) mother liquor configures:

1M HEPES:23.831gHEPES is dissolved in 80ml water, and effective pH range of HEPES is 6.8-8.2, is then used Distilled water is settled to 100mL；

3M sodium chloride: 17.55gNaCl is dissolved in 80mL water, is settled to 100mL with distilled water；

1M imidazoles: 6.808g imdazole is dissolved in 80mL water, is settled to 100mL with distilled water.

(2) solution is prepared

Binding Buffer:50mM HEPES；300mM sodium chloride；10mM imidazoles；PH=8.0.

Washing Buffer:50mM HEPES；300mM sodium chloride；50mM imidazoles；PH=8.0.

Elution Buffer:50mM HEPES；300mM sodium chloride；250mM imidazoles；PH=8.0.

(3) protein storage formula of liquid:

10mM Tris-HCl；200mM NaCl；1mM DTT；0.1mM EDTA；50%Glycerol；pH 7.4.

(4) protein purification

(a): 500ml bacterium solution being inoculated with the ratio of 1:1000, IPTG is added in 37 degree of cultures to OD 0.4-0.6, and IPTG is whole Concentration is 1mM, goes to 18 degree of culture 16-18h；

(b): cultured bacterium solution is gone in 50ml centrifuge tube, 4 DEG C of centrifugation 10min of 12000r, abandons supernatant；

(c): cell precipitation is resuspended with Binding Buffer, is placed on and breaks bacterium with ultrasonic cell disruptor on ice, works 8s stops 4s；Depending on broken time visual cell amount；

(d): 4 DEG C of centrifugation 8-10min of 12000r after ultrasonic disruption take supernatant, and supernatant is filtered with water phase pin type filter；

(e): ni-sepharose purification albumen column being cleaned up, upper Ni-NTA Agarose (QIAGEN), groundwater increment regards pillar tool Depending on body situation, 4 DEG C of standing 30min layerings, every column of filling is used 4-5 times；

(f): balancing pillar with 10-20ml Binding Buffer, filtered supernatant is then crossed into column；

(g): supernatant is all crossed after the completion of column, cleans pillar with 5-7ml Washing Buffer；

(h): add Elution Buffer to elute albumen, with Bradford method determination of protein concentration kit measurement concentration, Albumen is stored in protein storage liquid；

(i): taking 15 μ l protein samples, 5 μ l loadingbuffer are added, boiling makes albuminous degeneration, then SDS-PAGE Detect albumen quality.

Make Phos standard curve:

1. instrument and reagent: microplate reader (moral iron: HBS-1096A), KH2PO4, ammonium molybdate, malachite green, hydrochloric acid, Ttiton-100。

2. solution is prepared

(1): 2.5mM Phos titer: drying 2h in 80 DEG C of baking ovens for KH2PO4, weigh 0.34g KH2PO4, with DdH20 dissolves and is settled to 1L.

(2): 4.2% ammonium molybdate solution: ammonium molybdate 4.2g adds 4N HCl to dissolve and is settled to 100mL.

(3): MAT solution: being mixed with 0.045% malachite green solution and 4.2% ammonium molybdate solution by 3:1, stirring Ttiton-100 solution is added to final concentration 0.2% in 30min or more after filtering.

3. operating procedure

(1) 0,0.4,0.8,1.6,3.2,4,4.8,5.6,6.4,7.2,8,8.8,9.6,10.4,11.2 microlitre is taken respectively 2.5mM Phos titer supplies pure water to mixing well after 30 microlitres, is added sequentially 200 μ l respectively in 1.5ml EP pipe MAT solution sufficiently reacts 20min under room temperature, measures its OD660 value with microplate reader.

(2) make Phos standard curve: using the concentration of Phos as the abscissa of the standard curve, OD660 value is made For the ordinate of the standard curve.

Enzyme activity determination:

1. instrument and reagent: microplate reader (moral iron: HBS-1096A), glyphosate, substrate PEP (Sigma), substrate S3P (CAS Number:143393-03-7)。

2. operating procedure:

(1) 1.5ml centrifuge tube is placed on ice, is successively sequentially added into PEP solution (1000 μM of final concentration), S3P is molten Liquid (1000 μM of final concentration), 0.5M HEPES solution (final concentration 50mM), (NH4) 6MO7O24H2O solution (final concentration 0.1mM), distilled water is supplied to 20 μ l, is mixed, 28 DEG C of water-bath 5min；

(2) in 28 DEG C of water-bath after 5min, 10 μ l purifying protein (eggs are sequentially added into successively with the interval of every pipe 2s White concentration is 6.68ug/ml) mix after start timing, every pipe interval 2s is added sequentially 200 μ l MAT again after 28 DEG C of water-bath 10min Solution simultaneously mixes, and 20min is sufficiently reacted under room temperature, measures its OD660 value；

(3) calculate reaction speed V0: reaction speed refers to that the enzyme every milligram of substrate at 28 DEG C is inorganic produced by per minute The amount of phosphorus；

(4) 1.5ml centrifuge tube is placed on ice, is successively sequentially added into PEP solution (1000 μM of final concentration), S3P is molten Liquid (1000 μM of final concentration), 0.5M HEPES solution (final concentration 50mM), (NH4) 6MO7O24H2O solution (final concentration 0.1mM), glyphosate solution (10 μM of final concentration) supplies distilled water to 20 μ l, mixes, after 28 DEG C of water-bath 5min, it is pure that 10 μ l are added Change albumen (protein concentration 6.68ug/ml) and mix, 200 μ l MAT solution are added sequentially after 28 DEG C of water-bath 10min and mix, 20min is sufficiently reacted under room temperature, measures its OD660 value；

(5) calculate reaction speed Vi: reaction speed refers to that the enzyme every milligram of substrate at 28 DEG C is inorganic produced by per minute The amount of phosphorus.

TI is calculated:

According to bibliography (Lu et al., Protein Engineering, Design&Selection, 2017, Vol.30no.5, pp.395-399), active high, resistance an EPSPS mutant answers K with higher_cat、K_iWith it is lower K_m.Therefore, K_cat*K_i/K_mCan comprehensive assessment EPSPS mutant activity and resistance.This comprehensive parameters is named as herein TI (Tolerance Index) defines TI=K_cat*K_i/K_m.And TI=(v can be obtained by further calculating_i*v₀/(v₀-v_i))* [I]/([E] * [S]), wherein v₀Enzyme reaction rate, v when for no glyphosate_iTo there is enzyme reaction rate, [I] when glyphosate to be Glyphosate concentration, [E] are enzyme concentration, [S] is enzyme reaction substrate PEP concentration.

Experimental result is as shown in table 1.

Table 1

Sample name	OsEPSPS_WT	RA	RK	RQ
					V0μM	64.7	73.4	55.1	66.3
V10μM	20.7	59.5	41.7	38.9
					TI	2.2	22.6	12.4	6.8

It can be seen that according to the result of table 1

Enzyme reaction rate (V of the wild rice EPSPS OsEPSPS_WT in no glyphosate_0μM) be 64.7, RA, RK and The enzyme reaction rate of RQ under this condition is respectively that 73.4,55.1 and 66.3 and OsEPSPS_WT difference are little, explanation Basal enzyme kinetic parameter (the K of RA, RK and RQ mutant_cat、K_m) similar with OsEPSPS_WT.

When 10 μM of glyphosates are added in enzyme reaction, the enzyme reaction rate of wild rice EPSPS OsEPSPS_WT (V_10μM) it is 20.7, the enzyme reaction rate of RA, RK and RQ under this condition is respectively 59.5,41.7 and 38.9, hence it is evident that high In OsEPSPS_WT, illustrate that RA, RK and RQ mutant are substantially better than OsEPSPS_WT to the tolerance of glyphosate.Further Ground, the TI of OsEPSPS_WT are only 2.2, and the TI of RA is 22.6, are 10 times of the TI of OsEPSPS_WT, and the TI of RK is 12.4, It is about 6 times of the TI of OsEPSPS_WT, it is 3 times of the TI of OsEPSPS_WT that the TI of RQ, which is 6.8,.

Result above absolutely proves, compared with Escherichia coli EPSPS sequence, plant EPSPS provided in an embodiment of the present invention The amino acid sequence of mutant has in mutation L195A, L195K and L195Q in the 195th for corresponding to Escherichia coli EPSPS Any one mutation or combinations thereof, can assign or improve the glyphosate resistance of plant EPSPS mutant.

The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.

SEQUENCE LISTING

<110>Sichuan Tian Yuxing standing grain Biotechnology Co., Ltd

<120>a kind of plant EPSPS mutant being mutated containing A138X1 and 195X2 and its encoding gene and application

<160> 93

<170> PatentIn version 3.5

<210> 1

<211> 1365

<212> DNA

<213>artificial sequence

<400> 1

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtgcggatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaac taatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 2

<211> 1365

<212> DNA

<213>artificial sequence

<400> 2

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gttgcgatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaac taatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 3

<211> 1365

<212> DNA

<213>artificial sequence

<400> 3

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtgacgatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaac taatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 4

<211> 1365

<212> DNA

<213>artificial sequence

<400> 4

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtgaagatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaac taatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 5

<211> 1365

<212> DNA

<213>artificial sequence

<400> 5

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtatcgatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaac taatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 6

<211> 1365

<212> DNA

<213>artificial sequence

<400> 6

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtctagatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaac taatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 7

<211> 1365

<212> DNA

<213>artificial sequence

<400> 7

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtatggatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaac taatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 8

<211> 1365

<212> DNA

<213>artificial sequence

<400> 8

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtaacgatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaac taatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 9

<211> 1365

<212> DNA

<213>artificial sequence

<400> 9

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gttccgatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaac taatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 10

<211> 1365

<212> DNA

<213>artificial sequence

<400> 10

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct gcgactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtgcggatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaac taatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 11

<211> 1365

<212> DNA

<213>artificial sequence

<400> 11

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct gcgactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gttgcgatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaac taatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 12

<211> 1365

<212> DNA

<213>artificial sequence

<400> 12

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct gcgactgcaa tgcgatcctt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtgcggatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaac taatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 13

<211> 1365

<212> DNA

<213>artificial sequence

<400> 13

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct gcgactgcaa tgcgatcctt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gttgcgatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaac taatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 14

<211> 1359

<212> DNA

<213>artificial sequence

<400> 14

atggccgccg cagagaagcc ttcgacggcg ccggagatcg tgttggaacc tatcaaagac 60

atctcgggta ccatcacatt gccagggtct aagtctctgt ccaatcgaat tttgcttctt 120

gctgctctct ctgagggaac aactgttgta gacaacttgc tgtacagcga ggatattcat 180

tacatgcttg gtgcattaag gacccttgga ctgcgtgtgg aagacgacca aacaaccaaa 240

caagcaattg tggaaggctg tgggggattg tttcccacta ttaaagaatc taaagatgaa 300

atcaatttat tccttggaaa tgctggtact gcgatgcgtc ctttgacagc agctgtagtt 360

gctgcaggtg gaaatgcaag ctacgtactt gatggagtgc cccgaatgag agagaggcca 420

attggggatt tggttgctgg tcttaagcag ctcggtgcag atgttgattg ctttcttggc 480

acaaactgtc cacctgttcg tgtaaatggg aagggaggac ttcctggcgg aaaggtgaaa 540

ctgtctggat cagttagcag tcaataccta actgctttgc ttatggcagc tcctttagct 600

cttggcgatg tggaaattga gattgttgat aaactgattt ctgttccata tgttgaaatg 660

actctgaagt tgatggagcg ttttggagtt tctgtggaac acagtggtga ttgggataag 720

ttcttggtcc atggaggtca aaagtacaag tctcctggca atgcttttgt tgaaggtgat 780

gcttcaagtg ccagttactt cctagctggt gcagcagtta ctggtgggac tatcactgtt 840

aatggctgtg gcacaagcag tttacaggga gatgtaaaat ttgctgaagt tcttgaaaag 900

atgggagcta aggttacatg gtcagagaac agtgtcaccg ttactggacc gccacaagat 960

tcttctggtc aaaaagtctt gcaaggcatt gatgtcaata tgaacaagat gccagatgtt 1020

gccatgactc ttgccgttgt cgcactattt gctaatggtc aaactaccat cagagatgtg 1080

gcaagttgga gagttaaaga gactgagagg atgatagcaa tctgcacaga actcagaaag 1140

ctaggagcaa cagttgaaga aggtcctgat tactgtgtga ttactccacc tgagaaattg 1200

aatgtcacag ctatagacac atatgatgac cacagaatgg ccatggcatt ctctcttgct 1260

gcttgtgggg atgttccagt aaccatcaag gatcctggtt gcaccaggaa gacatttccc 1320

gactactttg aagtccttga gaggttcaca aggcactaa 1359

<210> 15

<211> 1359

<212> DNA

<213>artificial sequence

<400> 15

atggccgccg cagagaagcc ttcgacggcg ccggagatcg tgttggaacc tatcaaagac 60

atctcgggta ccatcacatt gccagggtct aagtctctgt ccaatcgaat tttgcttctt 120

gctgctctct ctgagggaac aactgttgta gacaacttgc tgtacagcga ggatattcat 180

tacatgcttg gtgcattaag gacccttgga ctgcgtgtgg aagacgacca aacaaccaaa 240

caagcaattg tggaaggctg tgggggattg tttcccacta ttaaagaatc taaagatgaa 300

atcaatttat tccttggaaa tgctgcaact gcgatgcgtc ctttgacagc agctgtagtt 360

gctgcaggtg gaaatgcaag ctacgtactt gatggagtgc cccgaatgag agagaggcca 420

attggggatt tggttgctgg tcttaagcag ctcggtgcag atgttgattg ctttcttggc 480

acaaactgtc cacctgttcg tgtaaatggg aagggaggac ttcctggcgg aaaggtgaaa 540

ctgtctggat cagttagcag tcaataccta actgctttgc ttatggcagc tcctttagct 600

cttggcgatg tggaaattga gattgttgat aaactgattt ctgttccata tgttgaaatg 660

actctgaagt tgatggagcg ttttggagtt tctgtggaac acagtggtga ttgggataag 720

ttcttggtcc atggaggtca aaagtacaag tctcctggca atgcttttgt tgaaggtgat 780

gcttcaagtg ccagttactt cctagctggt gcagcagtta ctggtgggac tatcactgtt 840

aatggctgtg gcacaagcag tttacaggga gatgtaaaat ttgctgaagt tcttgaaaag 900

atgggagcta aggttacatg gtcagagaac agtgtcaccg ttactggacc gccacaagat 960

tcttctggtc aaaaagtctt gcaaggcatt gatgtcaata tgaacaagat gccagatgtt 1020

gccatgactc ttgccgttgt cgcactattt gctaatggtc aaactaccat cagagatgtg 1080

gcaagttgga gagttaaaga gactgagagg atgatagcaa tctgcacaga actcagaaag 1140

ctaggagcaa cagttgaaga aggtcctgat tactgtgtga ttactccacc tgagaaattg 1200

aatgtcacag ctatagacac atatgatgac cacagaatgg ccatggcatt ctctcttgct 1260

gcttgtgggg atgttccagt aaccatcaag gatcctggtt gcaccaggaa gacatttccc 1320

gactactttg aagtccttga gaggttcaca aggcactaa 1359

<210> 16

<211> 1359

<212> DNA

<213>artificial sequence

<400> 16

atggccgccg cagagaagcc ttcgacggcg ccggagatcg tgttggaacc tatcaaagac 60

atctcgggta ccatcacatt gccagggtct aagtctctgt ccaatcgaat tttgcttctt 120

gctgctctct ctgagggaac aactgttgta gacaacttgc tgtacagcga ggatattcat 180

tacatgcttg gtgcattaag gacccttgga ctgcgtgtgg aagacgacca aacaaccaaa 240

caagcaattg tggaaggctg tgggggattg tttcccacta ttaaagaatc taaagatgaa 300

atcaatttat tccttggaaa tgctgcgact gcgatgcgtc ctttgacagc agctgtagtt 360

gctgcaggtg gaaatgcaag ctacgtactt gatggagtgc cccgaatgag agagaggcca 420

attggggatt tggttgctgg tcttaagcag ctcggttgcg atgttgattg ctttcttggc 480

acaaactgtc cacctgttcg tgtaaatggg aagggaggac ttcctggcgg aaaggtgaaa 540

ctgtctggat cagttagcag tcaataccta actgctttgc ttatggcagc tcctttagct 600

cttggcgatg tggaaattga gattgttgat aaactgattt ctgttccata tgttgaaatg 660

actctgaagt tgatggagcg ttttggagtt tctgtggaac acagtggtga ttgggataag 720

ttcttggtcc atggaggtca aaagtacaag tctcctggca atgcttttgt tgaaggtgat 780

gcttcaagtg ccagttactt cctagctggt gcagcagtta ctggtgggac tatcactgtt 840

aatggctgtg gcacaagcag tttacaggga gatgtaaaat ttgctgaagt tcttgaaaag 900

atgggagcta aggttacatg gtcagagaac agtgtcaccg ttactggacc gccacaagat 960

tcttctggtc aaaaagtctt gcaaggcatt gatgtcaata tgaacaagat gccagatgtt 1020

gccatgactc ttgccgttgt cgcactattt gctaatggtc aaactaccat cagagatgtg 1080

gcaagttgga gagttaaaga gactgagagg atgatagcaa tctgcacaga actcagaaag 1140

ctaggagcaa cagttgaaga aggtcctgat tactgtgtga ttactccacc tgagaaattg 1200

aatgtcacag ctatagacac atatgatgac cacagaatgg ccatggcatt ctctcttgct 1260

gcttgtgggg atgttccagt aaccatcaag gatcctggtt gcaccaggaa gacatttccc 1320

gactactttg aagtccttga gaggttcaca aggcactaa 1359

<210> 17

<211> 1359

<212> DNA

<213>artificial sequence

<400> 17

atggccgccg cagagaagcc ttcgacggcg ccggagatcg tgttggaacc tatcaaagac 60

atctcgggta ccatcacatt gccagggtct aagtctctgt ccaatcgaat tttgcttctt 120

gctgctctct ctgagggaac aactgttgta gacaacttgc tgtacagcga ggatattcat 180

tacatgcttg gtgcattaag gacccttgga ctgcgtgtgg aagacgacca aacaaccaaa 240

caagcaattg tggaaggctg tgggggattg tttcccacta ttaaagaatc taaagatgaa 300

atcaatttat tccttggaaa tgctgcgact gcgatgcgta gcttgacagc agctgtagtt 360

gctgcaggtg gaaatgcaag ctacgtactt gatggagtgc cccgaatgag agagaggcca 420

attggggatt tggttgctgg tcttaagcag ctcggtgcag atgttgattg ctttcttggc 480

acaaactgtc cacctgttcg tgtaaatggg aagggaggac ttcctggcgg aaaggtgaaa 540

ctgtctggat cagttagcag tcaataccta actgctttgc ttatggcagc tcctttagct 600

cttggcgatg tggaaattga gattgttgat aaactgattt ctgttccata tgttgaaatg 660

actctgaagt tgatggagcg ttttggagtt tctgtggaac acagtggtga ttgggataag 720

ttcttggtcc atggaggtca aaagtacaag tctcctggca atgcttttgt tgaaggtgat 780

gcttcaagtg ccagttactt cctagctggt gcagcagtta ctggtgggac tatcactgtt 840

aatggctgtg gcacaagcag tttacaggga gatgtaaaat ttgctgaagt tcttgaaaag 900

atgggagcta aggttacatg gtcagagaac agtgtcaccg ttactggacc gccacaagat 960

tcttctggtc aaaaagtctt gcaaggcatt gatgtcaata tgaacaagat gccagatgtt 1020

gccatgactc ttgccgttgt cgcactattt gctaatggtc aaactaccat cagagatgtg 1080

gcaagttgga gagttaaaga gactgagagg atgatagcaa tctgcacaga actcagaaag 1140

ctaggagcaa cagttgaaga aggtcctgat tactgtgtga ttactccacc tgagaaattg 1200

aatgtcacag ctatagacac atatgatgac cacagaatgg ccatggcatt ctctcttgct 1260

gcttgtgggg atgttccagt aaccatcaag gatcctggtt gcaccaggaa gacatttccc 1320

gactactttg aagtccttga gaggttcaca aggcactaa 1359

<210> 18

<211> 1359

<212> DNA

<213>artificial sequence

<400> 18

atggccgccg cagagaagcc ttcgacggcg ccggagatcg tgttggaacc tatcaaagac 60

atctcgggta ccatcacatt gccagggtct aagtctctgt ccaatcgaat tttgcttctt 120

gctgctctct ctgagggaac aactgttgta gacaacttgc tgtacagcga ggatattcat 180

tacatgcttg gtgcattaag gacccttgga ctgcgtgtgg aagacgacca aacaaccaaa 240

caagcaattg tggaaggctg tgggggattg tttcccacta ttaaagaatc taaagatgaa 300

atcaatttat tccttggaaa tgctgcgact gcgatgcgta gcttgacagc agctgtagtt 360

gctgcaggtg gaaatgcaag ctacgtactt gatggagtgc cccgaatgag agagaggcca 420

attggggatt tggttgctgg tcttaagcag ctcggtaacg atgttgattg ctttcttggc 480

acaaactgtc cacctgttcg tgtaaatggg aagggaggac ttcctggcgg aaaggtgaaa 540

ctgtctggat cagttagcag tcaataccta actgctttgc ttatggcagc tcctttagct 600

cttggcgatg tggaaattga gattgttgat aaactgattt ctgttccata tgttgaaatg 660

actctgaagt tgatggagcg ttttggagtt tctgtggaac acagtggtga ttgggataag 720

ttcttggtcc atggaggtca aaagtacaag tctcctggca atgcttttgt tgaaggtgat 780

gcttcaagtg ccagttactt cctagctggt gcagcagtta ctggtgggac tatcactgtt 840

aatggctgtg gcacaagcag tttacaggga gatgtaaaat ttgctgaagt tcttgaaaag 900

atgggagcta aggttacatg gtcagagaac agtgtcaccg ttactggacc gccacaagat 960

tcttctggtc aaaaagtctt gcaaggcatt gatgtcaata tgaacaagat gccagatgtt 1020

gccatgactc ttgccgttgt cgcactattt gctaatggtc aaactaccat cagagatgtg 1080

gcaagttgga gagttaaaga gactgagagg atgatagcaa tctgcacaga actcagaaag 1140

ctaggagcaa cagttgaaga aggtcctgat tactgtgtga ttactccacc tgagaaattg 1200

aatgtcacag ctatagacac atatgatgac cacagaatgg ccatggcatt ctctcttgct 1260

gcttgtgggg atgttccagt aaccatcaag gatcctggtt gcaccaggaa gacatttccc 1320

gactactttg aagtccttga gaggttcaca aggcactaa 1359

<210> 19

<211> 1371

<212> DNA

<213>artificial sequence

<400> 19

atggcgacgt ccgtggcggc ccccgcggcg cccgcgggcg ccgaggaggt cgtgctgcag 60

cccatccgcg agatctccgg cgccgtgcag ctgcccggct ccaagtcgct ctccaaccgg 120

atcctcctcc tctccgccct ctccgaggga acaacggtgg tggataacct gttgaacagt 180

gaggatgtcc actacatgct tgaggccctg gaagcccttg gactctccgt ggaagcagat 240

aaagttgcaa aaagagctgt ggttgttggc tgtggcggca ggttcccagt cgaaaaggac 300

gccaaagagg aagtaaagct cttcttgggt aatgctggaa ctgcaatgcg gccactgacg 360

gcagctgtag tagctgctgg tggaaatgca acttatgtgc ttgatggcgt accaagaatg 420

agggagcgac ctattggtga cttagttgta ggtttgcaac aactcggcgc agatgtcgat 480

tgtttccttg gcacaaactg cccacctgtc cgtatcaacg gcaaaggagg tctacctggt 540

ggcaaggtta agctctctgg ttccattagc agtcaatacc tgagttcctt gctgatggct 600

gctcctttgg ctcttgagga tgtcgagatt gaaatcattg ataaactgat ctccgttcct 660

tatgttgaaa tgacattgaa attgatggag cattttggtg tgactgcgga gcattctgat 720

agttgggaca gattctacat taagggagga caaaaataca agtcccctgg aaatgcctat 780

gtcgaaggtg atgcctcaag tgcgagctat ttcttggctg gtgctgccat caccggaggg 840

actgtgactg tcgaaggttg cggcaccact agtttgcagg gtgatgtgaa atttgctgag 900

gtacttgaaa tgatgggagc aaaggtcaca tggactgaca ctagtgtaac tgttactggc 960

ccaccgcgtc agccatttgg aaggaaacac ctaaaagctg ttgatgtcaa catgaacaaa 1020

atgccagatg tcgcgatgac tctagccgtt gttgccctgt ttgccgatgg tccaaccgct 1080

atcagagatg ttgcctcctg gagagtgaag gaaactgaaa gaatggtcgc gatccggacc 1140

gagctgacga agctgggagc aacggtggag gaaggcccgg actactgcat catcacgccg 1200

ccggagaagc tgaacatcac ggcgatcgac acctacgatg accaccggat ggcgatggcc 1260

ttctccctgg cggcctgtgc tgaggtgcca gtcaccatca gggaccctgg atgcacccga 1320

aagaccttcc ccaactactt cgacgtgcta agcaccttcg tcaagaacta g 1371

<210> 20

<211> 1371

<212> DNA

<213>artificial sequence

<400> 20

atggcgacgt ccgtggcggc ccccgcggcg cccgcgggcg ccgaggaggt cgtgctgcag 60

cccatccgcg agatctccgg cgccgtgcag ctgcccggct ccaagtcgct ctccaaccgg 120

atcctcctcc tctccgccct ctccgaggga acaacggtgg tggataacct gttgaacagt 180

gaggatgtcc actacatgct tgaggccctg gaagcccttg gactctccgt ggaagcagat 240

aaagttgcaa aaagagctgt ggttgttggc tgtggcggca ggttcccagt cgaaaaggac 300

gccaaagagg aagtaaagct cttcttgggt aatgctgcga ctgcaatgcg gagcctgacg 360

gcagctgtag tagctgctgg tggaaatgca acttatgtgc ttgatggcgt accaagaatg 420

agggagcgac ctattggtga cttagttgta ggtttgcaac aactcggcgc agatgtcgat 480

tgtttccttg gcacaaactg cccacctgtc cgtatcaacg gcaaaggagg tctacctggt 540

ggcaaggtta agctctctgg ttccattagc agtcaatacc tgagttcctt gctgatggct 600

gctcctttgg ctcttgagga tgtcgagatt gaaatcattg ataaactgat ctccgttcct 660

tatgttgaaa tgacattgaa attgatggag cattttggtg tgactgcgga gcattctgat 720

agttgggaca gattctacat taagggagga caaaaataca agtcccctgg aaatgcctat 780

gtcgaaggtg atgcctcaag tgcgagctat ttcttggctg gtgctgccat caccggaggg 840

actgtgactg tcgaaggttg cggcaccact agtttgcagg gtgatgtgaa atttgctgag 900

gtacttgaaa tgatgggagc aaaggtcaca tggactgaca ctagtgtaac tgttactggc 960

ccaccgcgtc agccatttgg aaggaaacac ctaaaagctg ttgatgtcaa catgaacaaa 1020

atgccagatg tcgcgatgac tctagccgtt gttgccctgt ttgccgatgg tccaaccgct 1080

atcagagatg ttgcctcctg gagagtgaag gaaactgaaa gaatggtcgc gatccggacc 1140

gagctgacga agctgggagc aacggtggag gaaggcccgg actactgcat catcacgccg 1200

ccggagaagc tgaacatcac ggcgatcgac acctacgatg accaccggat ggcgatggcc 1260

ttctccctgg cggcctgtgc tgaggtgcca gtcaccatca gggaccctgg atgcacccga 1320

aagaccttcc ccaactactt cgacgtgcta agcaccttcg tcaagaacta g 1371

<210> 21

<211> 1371

<212> DNA

<213>artificial sequence

<400> 21

atggcgacgt ccgtggcggc ccccgcggcg cccgcgggcg ccgaggaggt cgtgctgcag 60

cccatccgcg agatctccgg cgccgtgcag ctgcccggct ccaagtcgct ctccaaccgg 120

atcctcctcc tctccgccct ctccgaggga acaacggtgg tggataacct gttgaacagt 180

gaggatgtcc actacatgct tgaggccctg gaagcccttg gactctccgt ggaagcagat 240

aaagttgcaa aaagagctgt ggttgttggc tgtggcggca ggttcccagt cgaaaaggac 300

gccaaagagg aagtaaagct cttcttgggt aatgctgcga ctgcaatgcg gagcctgacg 360

gcagctgtag tagctgctgg tggaaatgca acttatgtgc ttgatggcgt accaagaatg 420

agggagcgac ctattggtga cttagttgta ggtttgcaac aactcggctg cgatgtcgat 480

tgtttccttg gcacaaactg cccacctgtc cgtatcaacg gcaaaggagg tctacctggt 540

ggcaaggtta agctctctgg ttccattagc agtcaatacc tgagttcctt gctgatggct 600

gctcctttgg ctcttgagga tgtcgagatt gaaatcattg ataaactgat ctccgttcct 660

tatgttgaaa tgacattgaa attgatggag cattttggtg tgactgcgga gcattctgat 720

agttgggaca gattctacat taagggagga caaaaataca agtcccctgg aaatgcctat 780

gtcgaaggtg atgcctcaag tgcgagctat ttcttggctg gtgctgccat caccggaggg 840

actgtgactg tcgaaggttg cggcaccact agtttgcagg gtgatgtgaa atttgctgag 900

gtacttgaaa tgatgggagc aaaggtcaca tggactgaca ctagtgtaac tgttactggc 960

ccaccgcgtc agccatttgg aaggaaacac ctaaaagctg ttgatgtcaa catgaacaaa 1020

atgccagatg tcgcgatgac tctagccgtt gttgccctgt ttgccgatgg tccaaccgct 1080

atcagagatg ttgcctcctg gagagtgaag gaaactgaaa gaatggtcgc gatccggacc 1140

gagctgacga agctgggagc aacggtggag gaaggcccgg actactgcat catcacgccg 1200

ccggagaagc tgaacatcac ggcgatcgac acctacgatg accaccggat ggcgatggcc 1260

ttctccctgg cggcctgtgc tgaggtgcca gtcaccatca gggaccctgg atgcacccga 1320

aagaccttcc ccaactactt cgacgtgcta agcaccttcg tcaagaacta g 1371

<210> 22

<211> 1365

<212> DNA

<213>artificial sequence

<400> 22

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtgcggatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaag caatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 23

<211> 1365

<212> DNA

<213>artificial sequence

<400> 23

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtgcggatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaat gcatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 24

<211> 1365

<212> DNA

<213>artificial sequence

<400> 24

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtgcggatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaag aaatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 25

<211> 1365

<212> DNA

<213>artificial sequence

<400> 25

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtgcggatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaat tcatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 26

<211> 1365

<212> DNA

<213>artificial sequence

<400> 26

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtgcggatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaag gaatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 27

<211> 1365

<212> DNA

<213>artificial sequence

<400> 27

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtgcggatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaac atatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 28

<211> 1365

<212> DNA

<213>artificial sequence

<400> 28

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtgcggatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaaa agatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 29

<211> 1365

<212> DNA

<213>artificial sequence

<400> 29

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtgcggatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaaa tgatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 30

<211> 1365

<212> DNA

<213>artificial sequence

<400> 30

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtgcggatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaaa acatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 31

<211> 1365

<212> DNA

<213>artificial sequence

<400> 31

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtgcggatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaac cgatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 32

<211> 1365

<212> DNA

<213>artificial sequence

<400> 32

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtgcggatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaac agatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 33

<211> 1365

<212> DNA

<213>artificial sequence

<400> 33

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtgcggatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaaa gaatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 34

<211> 1365

<212> DNA

<213>artificial sequence

<400> 34

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtgcggatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaat caatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 35

<211> 1365

<212> DNA

<213>artificial sequence

<400> 35

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtgcggatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaaa caatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 36

<211> 1365

<212> DNA

<213>artificial sequence

<400> 36

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtgcggatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaag taatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggtccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 37

<211> 1365

<212> DNA

<213>artificial sequence

<400> 37

atggcgtcca acgccgcggc tgcggcggcg aaggcggagg agatcgtgct ccagcccatc 60

agggagatct ccggggcggt tcagctgcca gggtccaagt cgctctccaa caggatcctc 120

ctcctctccg ccctctccga gggcacaaca gtggtggaca acttgctgaa cagtgaggat 180

gttcactaca tgcttgaggc cctgaaagcc ctcgggctct ctgtggaagc agataaagtt 240

gcaaaaagag ctgtagtcgt tggctgtggt ggcaagtttc ctgttgagaa ggatgcgaaa 300

gaggaagtgc aactcttctt ggggaacgct ggaactgcaa tgcgaccatt gacagcagcc 360

gtgactgctg ctggtggaaa tgcaacttat gtgcttgatg gagtgccacg aatgagggag 420

agaccgattg gtgacttggt tgtcgggttg aaacaacttg gtgcggatgt cgactgtttc 480

cttggcactg aatgcccacc tgttcgtgtc aagggaattg gaggacttcc tggtggcaag 540

gttaagctct ctggttccat cagcagtcag tacttgagtg ccttgctgat ggctgctcct 600

ttggcccttg gggatgtgga gatcgaaatc attgacaaat acatctccat tccttacgtt 660

gaaatgacat tgagattgat ggagcgtttt ggtgtgaagg cagagcattc tgatagttgg 720

gacagattct atattaaggg agggcagaag tacaaatctc ctggaaatgc ctatgttgaa 780

ggtgatgcct caagcgcgag ctatttcttg gctggtgctg caatcactgg aggcactgtg 840

acagttcaag gttgtggtac gaccagtttg cagggtgatg tcaaatttgc tgaggtactt 900

gagatgatgg gagcaaaggt tacatggact gacaccagtg taaccgtaac tggcccacca 960

cgtgagcctt atgggaagaa acacctgaaa gctgttgatg tcaacatgaa caaaatgcct 1020

gatgttgcca tgacccttgc cgttgttgca ctcttcgctg atggtccaac tgctatcaga 1080

gatgtggctt cctggagagt aaaggaaacc gaaaggatgg ttgcaattcg gaccgagcta 1140

acaaagctgg gagcatcggt tgaagaaggt cctgactact gcatcatcac cccaccggag 1200

aagctgaaca tcacggcaat cgacacctac gatgatcaca ggatggccat ggccttctcc 1260

ctcgctgcct gcgccgacgt gcccgtgacg atcagggacc ctggttgcac ccgcaagacc 1320

ttccccaact acttcgacgt tctaagcact ttcgtcagga actga 1365

<210> 38

<211> 1371

<212> DNA

<213>artificial sequence

<400> 38

atggcgacgt ccgtggcggc ccccgcggcg cccgcgggcg ccgaggaggt cgtgctgcag 60

cccatccgcg agatctccgg cgccgtgcag ctgcccggct ccaagtcgct ctccaaccgg 120

atcctcctcc tctccgccct ctccgaggga acaacggtgg tggataacct gttgaacagt 180

gaggatgtcc actacatgct tgaggccctg gaagcccttg gactctccgt ggaagcagat 240

aaagttgcaa aaagagctgt ggttgttggc tgtggcggca ggttcccagt cgaaaaggac 300

gccaaagagg aagtaaagct cttcttgggt aatgctggaa ctgcaatgcg gccactgacg 360

gcagctgtag tagctgctgg tggaaatgca acttatgtgc ttgatggcgt accaagaatg 420

agggagcgac ctattggtga cttagttgta ggtttgcaac aactcggcgc agatgtcgat 480

tgtttccttg gcacaaactg cccacctgtc cgtatcaacg gcaaaggagg tctacctggt 540

ggcaaggtta agctctctgg ttccattagc agtcaatacc tgagttcctt gctgatggct 600

gctcctttgg ctcttgagga tgtcgagatt gaaatcattg ataaagcaat ctccgttcct 660

tatgttgaaa tgacattgaa attgatggag cattttggtg tgactgcgga gcattctgat 720

agttgggaca gattctacat taagggagga caaaaataca agtcccctgg aaatgcctat 780

gtcgaaggtg atgcctcaag tgcgagctat ttcttggctg gtgctgccat caccggaggg 840

actgtgactg tcgaaggttg cggcaccact agtttgcagg gtgatgtgaa atttgctgag 900

gtacttgaaa tgatgggagc aaaggtcaca tggactgaca ctagtgtaac tgttactggc 960

ccaccgcgtc agccatttgg aaggaaacac ctaaaagctg ttgatgtcaa catgaacaaa 1020

atgccagatg tcgcgatgac tctagccgtt gttgccctgt ttgccgatgg tccaaccgct 1080

atcagagatg ttgcctcctg gagagtgaag gaaactgaaa gaatggtcgc gatccggacc 1140

gagctgacga agctgggagc aacggtggag gaaggcccgg actactgcat catcacgccg 1200

ccggagaagc tgaacatcac ggcgatcgac acctacgatg accaccggat ggcgatggcc 1260

ttctccctgg cggcctgtgc tgaggtgcca gtcaccatca gggaccctgg atgcacccga 1320

aagaccttcc ccaactactt cgacgtgcta agcaccttcg tcaagaacta g 1371

<210> 39

<211> 1371

<212> DNA

<213>artificial sequence

<400> 39

atggcgacgt ccgtggcggc ccccgcggcg cccgcgggcg ccgaggaggt cgtgctgcag 60

cccatccgcg agatctccgg cgccgtgcag ctgcccggct ccaagtcgct ctccaaccgg 120

atcctcctcc tctccgccct ctccgaggga acaacggtgg tggataacct gttgaacagt 180

gaggatgtcc actacatgct tgaggccctg gaagcccttg gactctccgt ggaagcagat 240

aaagttgcaa aaagagctgt ggttgttggc tgtggcggca ggttcccagt cgaaaaggac 300

gccaaagagg aagtaaagct cttcttgggt aatgctggaa ctgcaatgcg gccactgacg 360

gcagctgtag tagctgctgg tggaaatgca acttatgtgc ttgatggcgt accaagaatg 420

agggagcgac ctattggtga cttagttgta ggtttgcaac aactcggcgc agatgtcgat 480

tgtttccttg gcacaaactg cccacctgtc cgtatcaacg gcaaaggagg tctacctggt 540

ggcaaggtta agctctctgg ttccattagc agtcaatacc tgagttcctt gctgatggct 600

gctcctttgg ctcttgagga tgtcgagatt gaaatcattg ataaaggaat ctccgttcct 660

tatgttgaaa tgacattgaa attgatggag cattttggtg tgactgcgga gcattctgat 720

agttgggaca gattctacat taagggagga caaaaataca agtcccctgg aaatgcctat 780

gtcgaaggtg atgcctcaag tgcgagctat ttcttggctg gtgctgccat caccggaggg 840

actgtgactg tcgaaggttg cggcaccact agtttgcagg gtgatgtgaa atttgctgag 900

gtacttgaaa tgatgggagc aaaggtcaca tggactgaca ctagtgtaac tgttactggc 960

ccaccgcgtc agccatttgg aaggaaacac ctaaaagctg ttgatgtcaa catgaacaaa 1020

atgccagatg tcgcgatgac tctagccgtt gttgccctgt ttgccgatgg tccaaccgct 1080

atcagagatg ttgcctcctg gagagtgaag gaaactgaaa gaatggtcgc gatccggacc 1140

gagctgacga agctgggagc aacggtggag gaaggcccgg actactgcat catcacgccg 1200

ccggagaagc tgaacatcac ggcgatcgac acctacgatg accaccggat ggcgatggcc 1260

ttctccctgg cggcctgtgc tgaggtgcca gtcaccatca gggaccctgg atgcacccga 1320

aagaccttcc ccaactactt cgacgtgcta agcaccttcg tcaagaacta g 1371

<210> 40

<211> 1371

<212> DNA

<213>artificial sequence

<400> 40

atggcgacgt ccgtggcggc ccccgcggcg cccgcgggcg ccgaggaggt cgtgctgcag 60

cccatccgcg agatctccgg cgccgtgcag ctgcccggct ccaagtcgct ctccaaccgg 120

atcctcctcc tctccgccct ctccgaggga acaacggtgg tggataacct gttgaacagt 180

gaggatgtcc actacatgct tgaggccctg gaagcccttg gactctccgt ggaagcagat 240

aaagttgcaa aaagagctgt ggttgttggc tgtggcggca ggttcccagt cgaaaaggac 300

gccaaagagg aagtaaagct cttcttgggt aatgctggaa ctgcaatgcg gccactgacg 360

gcagctgtag tagctgctgg tggaaatgca acttatgtgc ttgatggcgt accaagaatg 420

agggagcgac ctattggtga cttagttgta ggtttgcaac aactcggcgc agatgtcgat 480

tgtttccttg gcacaaactg cccacctgtc cgtatcaacg gcaaaggagg tctacctggt 540

ggcaaggtta agctctctgg ttccattagc agtcaatacc tgagttcctt gctgatggct 600

gctcctttgg ctcttgagga tgtcgagatt gaaatcattg ataaaatgat ctccgttcct 660

tatgttgaaa tgacattgaa attgatggag cattttggtg tgactgcgga gcattctgat 720

agttgggaca gattctacat taagggagga caaaaataca agtcccctgg aaatgcctat 780

gtcgaaggtg atgcctcaag tgcgagctat ttcttggctg gtgctgccat caccggaggg 840

actgtgactg tcgaaggttg cggcaccact agtttgcagg gtgatgtgaa atttgctgag 900

gtacttgaaa tgatgggagc aaaggtcaca tggactgaca ctagtgtaac tgttactggc 960

ccaccgcgtc agccatttgg aaggaaacac ctaaaagctg ttgatgtcaa catgaacaaa 1020

atgccagatg tcgcgatgac tctagccgtt gttgccctgt ttgccgatgg tccaaccgct 1080

atcagagatg ttgcctcctg gagagtgaag gaaactgaaa gaatggtcgc gatccggacc 1140

gagctgacga agctgggagc aacggtggag gaaggcccgg actactgcat catcacgccg 1200

ccggagaagc tgaacatcac ggcgatcgac acctacgatg accaccggat ggcgatggcc 1260

ttctccctgg cggcctgtgc tgaggtgcca gtcaccatca gggaccctgg atgcacccga 1320

aagaccttcc ccaactactt cgacgtgcta agcaccttcg tcaagaacta g 1371

<210> 41

<211> 1371

<212> DNA

<213>artificial sequence

<400> 41

atggcgacgt ccgtggcggc ccccgcggcg cccgcgggcg ccgaggaggt cgtgctgcag 60

cccatccgcg agatctccgg cgccgtgcag ctgcccggct ccaagtcgct ctccaaccgg 120

atcctcctcc tctccgccct ctccgaggga acaacggtgg tggataacct gttgaacagt 180

gaggatgtcc actacatgct tgaggccctg gaagcccttg gactctccgt ggaagcagat 240

aaagttgcaa aaagagctgt ggttgttggc tgtggcggca ggttcccagt cgaaaaggac 300

gccaaagagg aagtaaagct cttcttgggt aatgctggaa ctgcaatgcg gccactgacg 360

gcagctgtag tagctgctgg tggaaatgca acttatgtgc ttgatggcgt accaagaatg 420

agggagcgac ctattggtga cttagttgta ggtttgcaac aactcggcgc agatgtcgat 480

tgtttccttg gcacaaactg cccacctgtc cgtatcaacg gcaaaggagg tctacctggt 540

ggcaaggtta agctctctgg ttccattagc agtcaatacc tgagttcctt gctgatggct 600

gctcctttgg ctcttgagga tgtcgagatt gaaatcattg ataaaccgat ctccgttcct 660

tatgttgaaa tgacattgaa attgatggag cattttggtg tgactgcgga gcattctgat 720

agttgggaca gattctacat taagggagga caaaaataca agtcccctgg aaatgcctat 780

gtcgaaggtg atgcctcaag tgcgagctat ttcttggctg gtgctgccat caccggaggg 840

actgtgactg tcgaaggttg cggcaccact agtttgcagg gtgatgtgaa atttgctgag 900

gtacttgaaa tgatgggagc aaaggtcaca tggactgaca ctagtgtaac tgttactggc 960

ccaccgcgtc agccatttgg aaggaaacac ctaaaagctg ttgatgtcaa catgaacaaa 1020

atgccagatg tcgcgatgac tctagccgtt gttgccctgt ttgccgatgg tccaaccgct 1080

atcagagatg ttgcctcctg gagagtgaag gaaactgaaa gaatggtcgc gatccggacc 1140

gagctgacga agctgggagc aacggtggag gaaggcccgg actactgcat catcacgccg 1200

ccggagaagc tgaacatcac ggcgatcgac acctacgatg accaccggat ggcgatggcc 1260

ttctccctgg cggcctgtgc tgaggtgcca gtcaccatca gggaccctgg atgcacccga 1320

aagaccttcc ccaactactt cgacgtgcta agcaccttcg tcaagaacta g 1371

<210> 42

<211> 1371

<212> DNA

<213>artificial sequence

<400> 42

atggcgacgt ccgtggcggc ccccgcggcg cccgcgggcg ccgaggaggt cgtgctgcag 60

cccatccgcg agatctccgg cgccgtgcag ctgcccggct ccaagtcgct ctccaaccgg 120

atcctcctcc tctccgccct ctccgaggga acaacggtgg tggataacct gttgaacagt 180

gaggatgtcc actacatgct tgaggccctg gaagcccttg gactctccgt ggaagcagat 240

aaagttgcaa aaagagctgt ggttgttggc tgtggcggca ggttcccagt cgaaaaggac 300

gccaaagagg aagtaaagct cttcttgggt aatgctggaa ctgcaatgcg gccactgacg 360

gcagctgtag tagctgctgg tggaaatgca acttatgtgc ttgatggcgt accaagaatg 420

agggagcgac ctattggtga cttagttgta ggtttgcaac aactcggcgc agatgtcgat 480

tgtttccttg gcacaaactg cccacctgtc cgtatcaacg gcaaaggagg tctacctggt 540

ggcaaggtta agctctctgg ttccattagc agtcaatacc tgagttcctt gctgatggct 600

gctcctttgg ctcttgagga tgtcgagatt gaaatcattg ataaacagat ctccgttcct 660

tatgttgaaa tgacattgaa attgatggag cattttggtg tgactgcgga gcattctgat 720

agttgggaca gattctacat taagggagga caaaaataca agtcccctgg aaatgcctat 780

gtcgaaggtg atgcctcaag tgcgagctat ttcttggctg gtgctgccat caccggaggg 840

actgtgactg tcgaaggttg cggcaccact agtttgcagg gtgatgtgaa atttgctgag 900

gtacttgaaa tgatgggagc aaaggtcaca tggactgaca ctagtgtaac tgttactggc 960

ccaccgcgtc agccatttgg aaggaaacac ctaaaagctg ttgatgtcaa catgaacaaa 1020

atgccagatg tcgcgatgac tctagccgtt gttgccctgt ttgccgatgg tccaaccgct 1080

atcagagatg ttgcctcctg gagagtgaag gaaactgaaa gaatggtcgc gatccggacc 1140

gagctgacga agctgggagc aacggtggag gaaggcccgg actactgcat catcacgccg 1200

ccggagaagc tgaacatcac ggcgatcgac acctacgatg accaccggat ggcgatggcc 1260

ttctccctgg cggcctgtgc tgaggtgcca gtcaccatca gggaccctgg atgcacccga 1320

aagaccttcc ccaactactt cgacgtgcta agcaccttcg tcaagaacta g 1371

<210> 43

<211> 1371

<212> DNA

<213>artificial sequence

<400> 43

atggcgacgt ccgtggcggc ccccgcggcg cccgcgggcg ccgaggaggt cgtgctgcag 60

cccatccgcg agatctccgg cgccgtgcag ctgcccggct ccaagtcgct ctccaaccgg 120

atcctcctcc tctccgccct ctccgaggga acaacggtgg tggataacct gttgaacagt 180

gaggatgtcc actacatgct tgaggccctg gaagcccttg gactctccgt ggaagcagat 240

aaagttgcaa aaagagctgt ggttgttggc tgtggcggca ggttcccagt cgaaaaggac 300

gccaaagagg aagtaaagct cttcttgggt aatgctggaa ctgcaatgcg gccactgacg 360

gcagctgtag tagctgctgg tggaaatgca acttatgtgc ttgatggcgt accaagaatg 420

agggagcgac ctattggtga cttagttgta ggtttgcaac aactcggcgc agatgtcgat 480

tgtttccttg gcacaaactg cccacctgtc cgtatcaacg gcaaaggagg tctacctggt 540

ggcaaggtta agctctctgg ttccattagc agtcaatacc tgagttcctt gctgatggct 600

gctcctttgg ctcttgagga tgtcgagatt gaaatcattg ataaatcaat ctccgttcct 660

tatgttgaaa tgacattgaa attgatggag cattttggtg tgactgcgga gcattctgat 720

agttgggaca gattctacat taagggagga caaaaataca agtcccctgg aaatgcctat 780

gtcgaaggtg atgcctcaag tgcgagctat ttcttggctg gtgctgccat caccggaggg 840

actgtgactg tcgaaggttg cggcaccact agtttgcagg gtgatgtgaa atttgctgag 900

gtacttgaaa tgatgggagc aaaggtcaca tggactgaca ctagtgtaac tgttactggc 960

ccaccgcgtc agccatttgg aaggaaacac ctaaaagctg ttgatgtcaa catgaacaaa 1020

atgccagatg tcgcgatgac tctagccgtt gttgccctgt ttgccgatgg tccaaccgct 1080

atcagagatg ttgcctcctg gagagtgaag gaaactgaaa gaatggtcgc gatccggacc 1140

gagctgacga agctgggagc aacggtggag gaaggcccgg actactgcat catcacgccg 1200

ccggagaagc tgaacatcac ggcgatcgac acctacgatg accaccggat ggcgatggcc 1260

ttctccctgg cggcctgtgc tgaggtgcca gtcaccatca gggaccctgg atgcacccga 1320

aagaccttcc ccaactactt cgacgtgcta agcaccttcg tcaagaacta g 1371

<210> 44

<211> 1359

<212> DNA

<213>artificial sequence

<400> 44

atggccgccg cagagaagcc ttcgacggcg ccggagatcg tgttggaacc tatcaaagac 60

atctcgggta ccatcacatt gccagggtct aagtctctgt ccaatcgaat tttgcttctt 120

gctgctctct ctgagggaac aactgttgta gacaacttgc tgtacagcga ggatattcat 180

tacatgcttg gtgcattaag gacccttgga ctgcgtgtgg aagacgacca aacaaccaaa 240

caagcaattg tggaaggctg tgggggattg tttcccacta ttaaagaatc taaagatgaa 300

atcaatttat tccttggaaa tgctggtact gcgatgcgtc ctttgacagc agctgtagtt 360

gctgcaggtg gaaatgcaag ctacgtactt gatggagtgc cccgaatgag agagaggcca 420

attggggatt tggttgctgg tcttaagcag ctcggtgcag atgttgattg ctttcttggc 480

acaaactgtc cacctgttcg tgtaaatggg aagggaggac ttcctggcgg aaaggtgaaa 540

ctgtctggat cagttagcag tcaataccta actgctttgc ttatggcagc tcctttagct 600

cttggcgatg tggaaattga gattgttgat aaaccgattt ctgttccata tgttgaaatg 660

actctgaagt tgatggagcg ttttggagtt tctgtggaac acagtggtga ttgggataag 720

ttcttggtcc atggaggtca aaagtacaag tctcctggca atgcttttgt tgaaggtgat 780

gcttcaagtg ccagttactt cctagctggt gcagcagtta ctggtgggac tatcactgtt 840

aatggctgtg gcacaagcag tttacaggga gatgtaaaat ttgctgaagt tcttgaaaag 900

atgggagcta aggttacatg gtcagagaac agtgtcaccg ttactggacc gccacaagat 960

tcttctggtc aaaaagtctt gcaaggcatt gatgtcaata tgaacaagat gccagatgtt 1020

gccatgactc ttgccgttgt cgcactattt gctaatggtc aaactaccat cagagatgtg 1080

gcaagttgga gagttaaaga gactgagagg atgatagcaa tctgcacaga actcagaaag 1140

ctaggagcaa cagttgaaga aggtcctgat tactgtgtga ttactccacc tgagaaattg 1200

aatgtcacag ctatagacac atatgatgac cacagaatgg ccatggcatt ctctcttgct 1260

gcttgtgggg atgttccagt aaccatcaag gatcctggtt gcaccaggaa gacatttccc 1320

gactactttg aagtccttga gaggttcaca aggcactaa 1359

<210> 45

<211> 1359

<212> DNA

<213>artificial sequence

<400> 45

atggccgccg cagagaagcc ttcgacggcg ccggagatcg tgttggaacc tatcaaagac 60

atctcgggta ccatcacatt gccagggtct aagtctctgt ccaatcgaat tttgcttctt 120

gctgctctct ctgagggaac aactgttgta gacaacttgc tgtacagcga ggatattcat 180

tacatgcttg gtgcattaag gacccttgga ctgcgtgtgg aagacgacca aacaaccaaa 240

caagcaattg tggaaggctg tgggggattg tttcccacta ttaaagaatc taaagatgaa 300

atcaatttat tccttggaaa tgctggtact gcgatgcgtc ctttgacagc agctgtagtt 360

gctgcaggtg gaaatgcaag ctacgtactt gatggagtgc cccgaatgag agagaggcca 420

attggggatt tggttgctgg tcttaagcag ctcggtgcag atgttgattg ctttcttggc 480

acaaactgtc cacctgttcg tgtaaatggg aagggaggac ttcctggcgg aaaggtgaaa 540

ctgtctggat cagttagcag tcaataccta actgctttgc ttatggcagc tcctttagct 600

cttggcgatg tggaaattga gattgttgat aaacagattt ctgttccata tgttgaaatg 660

actctgaagt tgatggagcg ttttggagtt tctgtggaac acagtggtga ttgggataag 720

ttcttggtcc atggaggtca aaagtacaag tctcctggca atgcttttgt tgaaggtgat 780

gcttcaagtg ccagttactt cctagctggt gcagcagtta ctggtgggac tatcactgtt 840

aatggctgtg gcacaagcag tttacaggga gatgtaaaat ttgctgaagt tcttgaaaag 900

atgggagcta aggttacatg gtcagagaac agtgtcaccg ttactggacc gccacaagat 960

tcttctggtc aaaaagtctt gcaaggcatt gatgtcaata tgaacaagat gccagatgtt 1020

gccatgactc ttgccgttgt cgcactattt gctaatggtc aaactaccat cagagatgtg 1080

gcaagttgga gagttaaaga gactgagagg atgatagcaa tctgcacaga actcagaaag 1140

ctaggagcaa cagttgaaga aggtcctgat tactgtgtga ttactccacc tgagaaattg 1200

aatgtcacag ctatagacac atatgatgac cacagaatgg ccatggcatt ctctcttgct 1260

gcttgtgggg atgttccagt aaccatcaag gatcctggtt gcaccaggaa gacatttccc 1320

gactactttg aagtccttga gaggttcaca aggcactaa 1359

<210> 46

<211> 1359

<212> DNA

<213>artificial sequence

<400> 46

atggccgccg cagagaagcc ttcgacggcg ccggagatcg tgttggaacc tatcaaagac 60

atctcgggta ccatcacatt gccagggtct aagtctctgt ccaatcgaat tttgcttctt 120

gctgctctct ctgagggaac aactgttgta gacaacttgc tgtacagcga ggatattcat 180

tacatgcttg gtgcattaag gacccttgga ctgcgtgtgg aagacgacca aacaaccaaa 240

caagcaattg tggaaggctg tgggggattg tttcccacta ttaaagaatc taaagatgaa 300

atcaatttat tccttggaaa tgctgcaact gcgatgcgtc ctttgacagc agctgtagtt 360

gctgcaggtg gaaatgcaag ctacgtactt gatggagtgc cccgaatgag agagaggcca 420

attggggatt tggttgctgg tcttaagcag ctcggtgcag atgttgattg ctttcttggc 480

acaaactgtc cacctgttcg tgtaaatggg aagggaggac ttcctggcgg aaaggtgaaa 540

ctgtctggat cagttagcag tcaataccta actgctttgc ttatggcagc tcctttagct 600

cttggcgatg tggaaattga gattgttgat aaaatgattt ctgttccata tgttgaaatg 660

actctgaagt tgatggagcg ttttggagtt tctgtggaac acagtggtga ttgggataag 720

ttcttggtcc atggaggtca aaagtacaag tctcctggca atgcttttgt tgaaggtgat 780

gcttcaagtg ccagttactt cctagctggt gcagcagtta ctggtgggac tatcactgtt 840

aatggctgtg gcacaagcag tttacaggga gatgtaaaat ttgctgaagt tcttgaaaag 900

atgggagcta aggttacatg gtcagagaac agtgtcaccg ttactggacc gccacaagat 960

tcttctggtc aaaaagtctt gcaaggcatt gatgtcaata tgaacaagat gccagatgtt 1020

gccatgactc ttgccgttgt cgcactattt gctaatggtc aaactaccat cagagatgtg 1080

gcaagttgga gagttaaaga gactgagagg atgatagcaa tctgcacaga actcagaaag 1140

ctaggagcaa cagttgaaga aggtcctgat tactgtgtga ttactccacc tgagaaattg 1200

aatgtcacag ctatagacac atatgatgac cacagaatgg ccatggcatt ctctcttgct 1260

gcttgtgggg atgttccagt aaccatcaag gatcctggtt gcaccaggaa gacatttccc 1320

gactactttg aagtccttga gaggttcaca aggcactaa 1359

<210> 47

<211> 427

<212> PRT

<213>artificial sequence

<400> 47

Met Glu Ser Leu Thr Leu Gln Pro Ile Ala Arg Val Asp Gly Thr Ile

1 5 10 15

Asn Leu Pro Gly Ser Lys Ser Val Ser Asn Arg Ala Leu Leu Leu Ala

20 25 30

Ala Leu Ala His Gly Lys Thr Val Leu Thr Asn Leu Leu Asp Ser Asp

35 40 45

Asp Val Arg His Met Leu Asn Ala Leu Thr Ala Leu Gly Val Ser Tyr

50 55 60

Thr Leu Ser Ala Asp Arg Thr Arg Cys Glu Ile Ile Gly Asn Gly Gly

65 70 75 80

Pro Leu His Ala Glu Gly Ala Leu Glu Leu Phe Leu Gly Asn Ala Gly

85 90 95

Thr Ala Met Arg Pro Leu Ala Ala Ala Leu Cys Leu Gly Ser Asn Asp

100 105 110

Ile Val Leu Thr Gly Glu Pro Arg Met Lys Glu Arg Pro Ile Gly His

115 120 125

Leu Val Asp Ala Leu Arg Leu Gly Gly Ala Lys Ile Thr Tyr Leu Glu

130 135 140

Gln Glu Asn Tyr Pro Pro Leu Arg Leu Gln Gly Gly Phe Thr Gly Gly

145 150 155 160

Asn Val Asp Val Asp Gly Ser Val Ser Ser Gln Phe Leu Thr Ala Leu

165 170 175

Leu Met Thr Ala Pro Leu Ala Pro Glu Asp Thr Val Ile Arg Ile Lys

180 185 190

Gly Asp Leu Val Ser Lys Pro Tyr Ile Asp Ile Thr Leu Asn Leu Met

195 200 205

Lys Thr Phe Gly Val Glu Ile Glu Asn Gln His Tyr Gln Gln Phe Val

210 215 220

Val Lys Gly Gly Gln Ser Tyr Gln Ser Pro Gly Thr Tyr Leu Val Glu

225 230 235 240

Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Ala Ala Ala Ile Lys

245 250 255

Gly Gly Thr Val Lys Val Thr Gly Ile Gly Arg Asn Ser Met Gln Gly

260 265 270

Asp Ile Arg Phe Ala Asp Val Leu Glu Lys Met Gly Ala Thr Ile Cys

275 280 285

Trp Gly Asp Asp Tyr Ile Ser Cys Thr Arg Gly Glu Leu Asn Ala Ile

290 295 300

Asp Met Asp Met Asn His Ile Pro Asp Ala Ala Met Thr Ile Ala Thr

305 310 315 320

Ala Ala Leu Phe Ala Lys Gly Thr Thr Thr Leu Arg Asn Ile Tyr Asn

325 330 335

Trp Arg Val Lys Glu Thr Asp Arg Leu Phe Ala Met Ala Thr Glu Leu

340 345 350

Arg Lys Val Gly Ala Glu Val Glu Glu Gly His Asp Tyr Ile Arg Ile

355 360 365

Thr Pro Pro Glu Lys Leu Asn Phe Ala Glu Ile Ala Thr Tyr Asn Asp

370 375 380

His Arg Met Ala Met Cys Phe Ser Leu Val Ala Leu Ser Asp Thr Pro

385 390 395 400

Val Thr Ile Leu Asp Pro Lys Cys Thr Ala Lys Thr Phe Pro Asp Tyr

405 410 415

Phe Glu Gln Leu Ala Arg Ile Ser Gln Ala Ala

420 425

<210> 48

<211> 454

<212> PRT

<213>artificial sequence

<400> 48

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Leu Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 49

<211> 454

<212> PRT

<213>artificial sequence

<400> 49

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Cys Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Leu Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 50

<211> 454

<212> PRT

<213>artificial sequence

<400> 50

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Asp Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Leu Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 51

<211> 454

<212> PRT

<213>artificial sequence

<400> 51

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Glu Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Leu Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 52

<211> 454

<212> PRT

<213>artificial sequence

<400> 52

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Ile Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Leu Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 53

<211> 454

<212> PRT

<213>artificial sequence

<400> 53

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Leu Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Leu Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 54

<211> 454

<212> PRT

<213>artificial sequence

<400> 54

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Met Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Leu Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 55

<211> 454

<212> PRT

<213>artificial sequence

<400> 55

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Asn Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Leu Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 56

<211> 454

<212> PRT

<213>artificial sequence

<400> 56

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Ser Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Leu Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 57

<211> 454

<212> PRT

<213>artificial sequence

<400> 57

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Ala Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Leu Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 58

<211> 454

<212> PRT

<213>artificial sequence

<400> 58

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Ala Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Cys Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Leu Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 59

<211> 454

<212> PRT

<213>artificial sequence

<400> 59

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Ala Thr

100 105 110

Ala Met Arg Ser Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Leu Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 60

<211> 454

<212> PRT

<213>artificial sequence

<400> 60

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Ala Thr

100 105 110

Ala Met Arg Ser Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Cys Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Leu Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 61

<211> 452

<212> PRT

<213>artificial sequence

<400> 61

Met Ala Ala Ala Glu Lys Pro Ser Thr Ala Pro Glu Ile Val Leu Glu

1 5 10 15

Pro Ile Lys Asp Ile Ser Gly Thr Ile Thr Leu Pro Gly Ser Lys Ser

20 25 30

Leu Ser Asn Arg Ile Leu Leu Leu Ala Ala Leu Ser Glu Gly Thr Thr

35 40 45

Val Val Asp Asn Leu Leu Tyr Ser Glu Asp Ile His Tyr Met Leu Gly

50 55 60

Ala Leu Arg Thr Leu Gly Leu Arg Val Glu Asp Asp Gln Thr Thr Lys

65 70 75 80

Gln Ala Ile Val Glu Gly Cys Gly Gly Leu Phe Pro Thr Ile Lys Glu

85 90 95

Ser Lys Asp Glu Ile Asn Leu Phe Leu Gly Asn Ala Gly Thr Ala Met

100 105 110

Arg Pro Leu Thr Ala Ala Val Val Ala Ala Gly Gly Asn Ala Ser Tyr

115 120 125

Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly Asp Leu

130 135 140

Val Ala Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe Leu Gly

145 150 155 160

Thr Asn Cys Pro Pro Val Arg Val Asn Gly Lys Gly Gly Leu Pro Gly

165 170 175

Gly Lys Val Lys Leu Ser Gly Ser Val Ser Ser Gln Tyr Leu Thr Ala

180 185 190

Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile Glu Ile

195 200 205

Val Asp Lys Leu Ile Ser Val Pro Tyr Val Glu Met Thr Leu Lys Leu

210 215 220

Met Glu Arg Phe Gly Val Ser Val Glu His Ser Gly Asp Trp Asp Lys

225 230 235 240

Phe Leu Val His Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn Ala Phe

245 250 255

Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly Ala Ala

260 265 270

Val Thr Gly Gly Thr Ile Thr Val Asn Gly Cys Gly Thr Ser Ser Leu

275 280 285

Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Lys Met Gly Ala Lys

290 295 300

Val Thr Trp Ser Glu Asn Ser Val Thr Val Thr Gly Pro Pro Gln Asp

305 310 315 320

Ser Ser Gly Gln Lys Val Leu Gln Gly Ile Asp Val Asn Met Asn Lys

325 330 335

Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe Ala Asn

340 345 350

Gly Gln Thr Thr Ile Arg Asp Val Ala Ser Trp Arg Val Lys Glu Thr

355 360 365

Glu Arg Met Ile Ala Ile Cys Thr Glu Leu Arg Lys Leu Gly Ala Thr

370 375 380

Val Glu Glu Gly Pro Asp Tyr Cys Val Ile Thr Pro Pro Glu Lys Leu

385 390 395 400

Asn Val Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala Met Ala

405 410 415

Phe Ser Leu Ala Ala Cys Gly Asp Val Pro Val Thr Ile Lys Asp Pro

420 425 430

Gly Cys Thr Arg Lys Thr Phe Pro Asp Tyr Phe Glu Val Leu Glu Arg

435 440 445

Phe Thr Arg His

450

<210> 62

<211> 452

<212> PRT

<213>artificial sequence

<400> 62

Met Ala Ala Ala Glu Lys Pro Ser Thr Ala Pro Glu Ile Val Leu Glu

1 5 10 15

Pro Ile Lys Asp Ile Ser Gly Thr Ile Thr Leu Pro Gly Ser Lys Ser

20 25 30

Leu Ser Asn Arg Ile Leu Leu Leu Ala Ala Leu Ser Glu Gly Thr Thr

35 40 45

Val Val Asp Asn Leu Leu Tyr Ser Glu Asp Ile His Tyr Met Leu Gly

50 55 60

Ala Leu Arg Thr Leu Gly Leu Arg Val Glu Asp Asp Gln Thr Thr Lys

65 70 75 80

Gln Ala Ile Val Glu Gly Cys Gly Gly Leu Phe Pro Thr Ile Lys Glu

85 90 95

Ser Lys Asp Glu Ile Asn Leu Phe Leu Gly Asn Ala Ala Thr Ala Met

100 105 110

Arg Pro Leu Thr Ala Ala Val Val Ala Ala Gly Gly Asn Ala Ser Tyr

115 120 125

Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly Asp Leu

130 135 140

Val Ala Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe Leu Gly

145 150 155 160

Thr Asn Cys Pro Pro Val Arg Val Asn Gly Lys Gly Gly Leu Pro Gly

165 170 175

Gly Lys Val Lys Leu Ser Gly Ser Val Ser Ser Gln Tyr Leu Thr Ala

180 185 190

Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile Glu Ile

195 200 205

Val Asp Lys Leu Ile Ser Val Pro Tyr Val Glu Met Thr Leu Lys Leu

210 215 220

Met Glu Arg Phe Gly Val Ser Val Glu His Ser Gly Asp Trp Asp Lys

225 230 235 240

Phe Leu Val His Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn Ala Phe

245 250 255

Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly Ala Ala

260 265 270

Val Thr Gly Gly Thr Ile Thr Val Asn Gly Cys Gly Thr Ser Ser Leu

275 280 285

Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Lys Met Gly Ala Lys

290 295 300

Val Thr Trp Ser Glu Asn Ser Val Thr Val Thr Gly Pro Pro Gln Asp

305 310 315 320

Ser Ser Gly Gln Lys Val Leu Gln Gly Ile Asp Val Asn Met Asn Lys

325 330 335

Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe Ala Asn

340 345 350

Gly Gln Thr Thr Ile Arg Asp Val Ala Ser Trp Arg Val Lys Glu Thr

355 360 365

Glu Arg Met Ile Ala Ile Cys Thr Glu Leu Arg Lys Leu Gly Ala Thr

370 375 380

Val Glu Glu Gly Pro Asp Tyr Cys Val Ile Thr Pro Pro Glu Lys Leu

385 390 395 400

Asn Val Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala Met Ala

405 410 415

Phe Ser Leu Ala Ala Cys Gly Asp Val Pro Val Thr Ile Lys Asp Pro

420 425 430

Gly Cys Thr Arg Lys Thr Phe Pro Asp Tyr Phe Glu Val Leu Glu Arg

435 440 445

Phe Thr Arg His

450

<210> 63

<211> 452

<212> PRT

<213>artificial sequence

<400> 63

Met Ala Ala Ala Glu Lys Pro Ser Thr Ala Pro Glu Ile Val Leu Glu

1 5 10 15

Pro Ile Lys Asp Ile Ser Gly Thr Ile Thr Leu Pro Gly Ser Lys Ser

20 25 30

Leu Ser Asn Arg Ile Leu Leu Leu Ala Ala Leu Ser Glu Gly Thr Thr

35 40 45

Val Val Asp Asn Leu Leu Tyr Ser Glu Asp Ile His Tyr Met Leu Gly

50 55 60

Ala Leu Arg Thr Leu Gly Leu Arg Val Glu Asp Asp Gln Thr Thr Lys

65 70 75 80

Gln Ala Ile Val Glu Gly Cys Gly Gly Leu Phe Pro Thr Ile Lys Glu

85 90 95

Ser Lys Asp Glu Ile Asn Leu Phe Leu Gly Asn Ala Ala Thr Ala Met

100 105 110

Arg Pro Leu Thr Ala Ala Val Val Ala Ala Gly Gly Asn Ala Ser Tyr

115 120 125

Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly Asp Leu

130 135 140

Val Ala Gly Leu Lys Gln Leu Gly Cys Asp Val Asp Cys Phe Leu Gly

145 150 155 160

Thr Asn Cys Pro Pro Val Arg Val Asn Gly Lys Gly Gly Leu Pro Gly

165 170 175

Gly Lys Val Lys Leu Ser Gly Ser Val Ser Ser Gln Tyr Leu Thr Ala

180 185 190

Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile Glu Ile

195 200 205

Val Asp Lys Leu Ile Ser Val Pro Tyr Val Glu Met Thr Leu Lys Leu

210 215 220

Met Glu Arg Phe Gly Val Ser Val Glu His Ser Gly Asp Trp Asp Lys

225 230 235 240

Phe Leu Val His Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn Ala Phe

245 250 255

Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly Ala Ala

260 265 270

Val Thr Gly Gly Thr Ile Thr Val Asn Gly Cys Gly Thr Ser Ser Leu

275 280 285

Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Lys Met Gly Ala Lys

290 295 300

Val Thr Trp Ser Glu Asn Ser Val Thr Val Thr Gly Pro Pro Gln Asp

305 310 315 320

Ser Ser Gly Gln Lys Val Leu Gln Gly Ile Asp Val Asn Met Asn Lys

325 330 335

Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe Ala Asn

340 345 350

Gly Gln Thr Thr Ile Arg Asp Val Ala Ser Trp Arg Val Lys Glu Thr

355 360 365

Glu Arg Met Ile Ala Ile Cys Thr Glu Leu Arg Lys Leu Gly Ala Thr

370 375 380

Val Glu Glu Gly Pro Asp Tyr Cys Val Ile Thr Pro Pro Glu Lys Leu

385 390 395 400

Asn Val Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala Met Ala

405 410 415

Phe Ser Leu Ala Ala Cys Gly Asp Val Pro Val Thr Ile Lys Asp Pro

420 425 430

Gly Cys Thr Arg Lys Thr Phe Pro Asp Tyr Phe Glu Val Leu Glu Arg

435 440 445

Phe Thr Arg His

450

<210> 64

<211> 452

<212> PRT

<213>artificial sequence

<400> 64

Met Ala Ala Ala Glu Lys Pro Ser Thr Ala Pro Glu Ile Val Leu Glu

1 5 10 15

Pro Ile Lys Asp Ile Ser Gly Thr Ile Thr Leu Pro Gly Ser Lys Ser

20 25 30

Leu Ser Asn Arg Ile Leu Leu Leu Ala Ala Leu Ser Glu Gly Thr Thr

35 40 45

Val Val Asp Asn Leu Leu Tyr Ser Glu Asp Ile His Tyr Met Leu Gly

50 55 60

Ala Leu Arg Thr Leu Gly Leu Arg Val Glu Asp Asp Gln Thr Thr Lys

65 70 75 80

Gln Ala Ile Val Glu Gly Cys Gly Gly Leu Phe Pro Thr Ile Lys Glu

85 90 95

Ser Lys Asp Glu Ile Asn Leu Phe Leu Gly Asn Ala Ala Thr Ala Met

100 105 110

Arg Ser Leu Thr Ala Ala Val Val Ala Ala Gly Gly Asn Ala Ser Tyr

115 120 125

Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly Asp Leu

130 135 140

Val Ala Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe Leu Gly

145 150 155 160

Thr Asn Cys Pro Pro Val Arg Val Asn Gly Lys Gly Gly Leu Pro Gly

165 170 175

Gly Lys Val Lys Leu Ser Gly Ser Val Ser Ser Gln Tyr Leu Thr Ala

180 185 190

Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile Glu Ile

195 200 205

Val Asp Lys Leu Ile Ser Val Pro Tyr Val Glu Met Thr Leu Lys Leu

210 215 220

Met Glu Arg Phe Gly Val Ser Val Glu His Ser Gly Asp Trp Asp Lys

225 230 235 240

Phe Leu Val His Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn Ala Phe

245 250 255

Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly Ala Ala

260 265 270

Val Thr Gly Gly Thr Ile Thr Val Asn Gly Cys Gly Thr Ser Ser Leu

275 280 285

Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Lys Met Gly Ala Lys

290 295 300

Val Thr Trp Ser Glu Asn Ser Val Thr Val Thr Gly Pro Pro Gln Asp

305 310 315 320

Ser Ser Gly Gln Lys Val Leu Gln Gly Ile Asp Val Asn Met Asn Lys

325 330 335

Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe Ala Asn

340 345 350

Gly Gln Thr Thr Ile Arg Asp Val Ala Ser Trp Arg Val Lys Glu Thr

355 360 365

Glu Arg Met Ile Ala Ile Cys Thr Glu Leu Arg Lys Leu Gly Ala Thr

370 375 380

Val Glu Glu Gly Pro Asp Tyr Cys Val Ile Thr Pro Pro Glu Lys Leu

385 390 395 400

Asn Val Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala Met Ala

405 410 415

Phe Ser Leu Ala Ala Cys Gly Asp Val Pro Val Thr Ile Lys Asp Pro

420 425 430

Gly Cys Thr Arg Lys Thr Phe Pro Asp Tyr Phe Glu Val Leu Glu Arg

435 440 445

Phe Thr Arg His

450

<210> 65

<211> 452

<212> PRT

<213>artificial sequence

<400> 65

Met Ala Ala Ala Glu Lys Pro Ser Thr Ala Pro Glu Ile Val Leu Glu

1 5 10 15

Pro Ile Lys Asp Ile Ser Gly Thr Ile Thr Leu Pro Gly Ser Lys Ser

20 25 30

Leu Ser Asn Arg Ile Leu Leu Leu Ala Ala Leu Ser Glu Gly Thr Thr

35 40 45

Val Val Asp Asn Leu Leu Tyr Ser Glu Asp Ile His Tyr Met Leu Gly

50 55 60

Ala Leu Arg Thr Leu Gly Leu Arg Val Glu Asp Asp Gln Thr Thr Lys

65 70 75 80

Gln Ala Ile Val Glu Gly Cys Gly Gly Leu Phe Pro Thr Ile Lys Glu

85 90 95

Ser Lys Asp Glu Ile Asn Leu Phe Leu Gly Asn Ala Ala Thr Ala Met

100 105 110

Arg Ser Leu Thr Ala Ala Val Val Ala Ala Gly Gly Asn Ala Ser Tyr

115 120 125

Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly Asp Leu

130 135 140

Val Ala Gly Leu Lys Gln Leu Gly Asn Asp Val Asp Cys Phe Leu Gly

145 150 155 160

Thr Asn Cys Pro Pro Val Arg Val Asn Gly Lys Gly Gly Leu Pro Gly

165 170 175

Gly Lys Val Lys Leu Ser Gly Ser Val Ser Ser Gln Tyr Leu Thr Ala

180 185 190

Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile Glu Ile

195 200 205

Val Asp Lys Leu Ile Ser Val Pro Tyr Val Glu Met Thr Leu Lys Leu

210 215 220

Met Glu Arg Phe Gly Val Ser Val Glu His Ser Gly Asp Trp Asp Lys

225 230 235 240

Phe Leu Val His Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn Ala Phe

245 250 255

Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly Ala Ala

260 265 270

Val Thr Gly Gly Thr Ile Thr Val Asn Gly Cys Gly Thr Ser Ser Leu

275 280 285

Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Lys Met Gly Ala Lys

290 295 300

Val Thr Trp Ser Glu Asn Ser Val Thr Val Thr Gly Pro Pro Gln Asp

305 310 315 320

Ser Ser Gly Gln Lys Val Leu Gln Gly Ile Asp Val Asn Met Asn Lys

325 330 335

Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe Ala Asn

340 345 350

Gly Gln Thr Thr Ile Arg Asp Val Ala Ser Trp Arg Val Lys Glu Thr

355 360 365

Glu Arg Met Ile Ala Ile Cys Thr Glu Leu Arg Lys Leu Gly Ala Thr

370 375 380

Val Glu Glu Gly Pro Asp Tyr Cys Val Ile Thr Pro Pro Glu Lys Leu

385 390 395 400

Asn Val Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala Met Ala

405 410 415

Phe Ser Leu Ala Ala Cys Gly Asp Val Pro Val Thr Ile Lys Asp Pro

420 425 430

Gly Cys Thr Arg Lys Thr Phe Pro Asp Tyr Phe Glu Val Leu Glu Arg

435 440 445

Phe Thr Arg His

450

<210> 66

<211> 456

<212> PRT

<213>artificial sequence

<400> 66

Met Ala Thr Ser Val Ala Ala Pro Ala Ala Pro Ala Gly Ala Glu Glu

1 5 10 15

Val Val Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro

20 25 30

Gly Ser Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser

35 40 45

Glu Gly Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His

50 55 60

Tyr Met Leu Glu Ala Leu Glu Ala Leu Gly Leu Ser Val Glu Ala Asp

65 70 75 80

Lys Val Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Arg Phe Pro

85 90 95

Val Glu Lys Asp Ala Lys Glu Glu Val Lys Leu Phe Leu Gly Asn Ala

100 105 110

Gly Thr Ala Met Arg Pro Leu Thr Ala Ala Val Val Ala Ala Gly Gly

115 120 125

Asn Ala Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro

130 135 140

Ile Gly Asp Leu Val Val Gly Leu Gln Gln Leu Gly Ala Asp Val Asp

145 150 155 160

Cys Phe Leu Gly Thr Asn Cys Pro Pro Val Arg Ile Asn Gly Lys Gly

165 170 175

Gly Leu Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln

180 185 190

Tyr Leu Ser Ser Leu Leu Met Ala Ala Pro Leu Ala Leu Glu Asp Val

195 200 205

Glu Ile Glu Ile Ile Asp Lys Leu Ile Ser Val Pro Tyr Val Glu Met

210 215 220

Thr Leu Lys Leu Met Glu His Phe Gly Val Thr Ala Glu His Ser Asp

225 230 235 240

Ser Trp Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro

245 250 255

Gly Asn Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu

260 265 270

Ala Gly Ala Ala Ile Thr Gly Gly Thr Val Thr Val Glu Gly Cys Gly

275 280 285

Thr Thr Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met

290 295 300

Met Gly Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly

305 310 315 320

Pro Pro Arg Gln Pro Phe Gly Arg Lys His Leu Lys Ala Val Asp Val

325 330 335

Asn Met Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala

340 345 350

Leu Phe Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg

355 360 365

Val Lys Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys

370 375 380

Leu Gly Ala Thr Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro

385 390 395 400

Pro Glu Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg

405 410 415

Met Ala Met Ala Phe Ser Leu Ala Ala Cys Ala Glu Val Pro Val Thr

420 425 430

Ile Arg Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp

435 440 445

Val Leu Ser Thr Phe Val Lys Asn

450 455

<210> 67

<211> 456

<212> PRT

<213>artificial sequence

<400> 67

Met Ala Thr Ser Val Ala Ala Pro Ala Ala Pro Ala Gly Ala Glu Glu

1 5 10 15

Val Val Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro

20 25 30

Gly Ser Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser

35 40 45

Glu Gly Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His

50 55 60

Tyr Met Leu Glu Ala Leu Glu Ala Leu Gly Leu Ser Val Glu Ala Asp

65 70 75 80

Lys Val Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Arg Phe Pro

85 90 95

Val Glu Lys Asp Ala Lys Glu Glu Val Lys Leu Phe Leu Gly Asn Ala

100 105 110

Ala Thr Ala Met Arg Ser Leu Thr Ala Ala Val Val Ala Ala Gly Gly

115 120 125

Asn Ala Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro

130 135 140

Ile Gly Asp Leu Val Val Gly Leu Gln Gln Leu Gly Ala Asp Val Asp

145 150 155 160

Cys Phe Leu Gly Thr Asn Cys Pro Pro Val Arg Ile Asn Gly Lys Gly

165 170 175

Gly Leu Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln

180 185 190

Tyr Leu Ser Ser Leu Leu Met Ala Ala Pro Leu Ala Leu Glu Asp Val

195 200 205

Glu Ile Glu Ile Ile Asp Lys Leu Ile Ser Val Pro Tyr Val Glu Met

210 215 220

Thr Leu Lys Leu Met Glu His Phe Gly Val Thr Ala Glu His Ser Asp

225 230 235 240

Ser Trp Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro

245 250 255

Gly Asn Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu

260 265 270

Ala Gly Ala Ala Ile Thr Gly Gly Thr Val Thr Val Glu Gly Cys Gly

275 280 285

Thr Thr Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met

290 295 300

Met Gly Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly

305 310 315 320

Pro Pro Arg Gln Pro Phe Gly Arg Lys His Leu Lys Ala Val Asp Val

325 330 335

Asn Met Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala

340 345 350

Leu Phe Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg

355 360 365

Val Lys Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys

370 375 380

Leu Gly Ala Thr Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro

385 390 395 400

Pro Glu Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg

405 410 415

Met Ala Met Ala Phe Ser Leu Ala Ala Cys Ala Glu Val Pro Val Thr

420 425 430

Ile Arg Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp

435 440 445

Val Leu Ser Thr Phe Val Lys Asn

450 455

<210> 68

<211> 456

<212> PRT

<213>artificial sequence

<400> 68

Met Ala Thr Ser Val Ala Ala Pro Ala Ala Pro Ala Gly Ala Glu Glu

1 5 10 15

Val Val Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro

20 25 30

Gly Ser Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser

35 40 45

Glu Gly Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His

50 55 60

Tyr Met Leu Glu Ala Leu Glu Ala Leu Gly Leu Ser Val Glu Ala Asp

65 70 75 80

Lys Val Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Arg Phe Pro

85 90 95

Val Glu Lys Asp Ala Lys Glu Glu Val Lys Leu Phe Leu Gly Asn Ala

100 105 110

Ala Thr Ala Met Arg Ser Leu Thr Ala Ala Val Val Ala Ala Gly Gly

115 120 125

Asn Ala Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro

130 135 140

Ile Gly Asp Leu Val Val Gly Leu Gln Gln Leu Gly Cys Asp Val Asp

145 150 155 160

Cys Phe Leu Gly Thr Asn Cys Pro Pro Val Arg Ile Asn Gly Lys Gly

165 170 175

Gly Leu Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln

180 185 190

Tyr Leu Ser Ser Leu Leu Met Ala Ala Pro Leu Ala Leu Glu Asp Val

195 200 205

Glu Ile Glu Ile Ile Asp Lys Leu Ile Ser Val Pro Tyr Val Glu Met

210 215 220

Thr Leu Lys Leu Met Glu His Phe Gly Val Thr Ala Glu His Ser Asp

225 230 235 240

Ser Trp Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro

245 250 255

Gly Asn Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu

260 265 270

Ala Gly Ala Ala Ile Thr Gly Gly Thr Val Thr Val Glu Gly Cys Gly

275 280 285

Thr Thr Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met

290 295 300

Met Gly Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly

305 310 315 320

Pro Pro Arg Gln Pro Phe Gly Arg Lys His Leu Lys Ala Val Asp Val

325 330 335

Asn Met Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala

340 345 350

Leu Phe Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg

355 360 365

Val Lys Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys

370 375 380

Leu Gly Ala Thr Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro

385 390 395 400

Pro Glu Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg

405 410 415

Met Ala Met Ala Phe Ser Leu Ala Ala Cys Ala Glu Val Pro Val Thr

420 425 430

Ile Arg Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp

435 440 445

Val Leu Ser Thr Phe Val Lys Asn

450 455

<210> 69

<211> 454

<212> PRT

<213>artificial sequence

<400> 69

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Ala Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 70

<211> 454

<212> PRT

<213>artificial sequence

<400> 70

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Cys Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 71

<211> 454

<212> PRT

<213>artificial sequence

<400> 71

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Glu Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 72

<211> 454

<212> PRT

<213>artificial sequence

<400> 72

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Phe Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 73

<211> 454

<212> PRT

<213>artificial sequence

<400> 73

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Gly Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 74

<211> 454

<212> PRT

<213>artificial sequence

<400> 74

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys His Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 75

<211> 454

<212> PRT

<213>artificial sequence

<400> 75

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Lys Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 76

<211> 454

<212> PRT

<213>artificial sequence

<400> 76

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Met Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 77

<211> 454

<212> PRT

<213>artificial sequence

<400> 77

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Asn Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 78

<211> 454

<212> PRT

<213>artificial sequence

<400> 78

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Pro Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 79

<211> 454

<212> PRT

<213>artificial sequence

<400> 79

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Gln Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 80

<211> 454

<212> PRT

<213>artificial sequence

<400> 80

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Arg Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 81

<211> 454

<212> PRT

<213>artificial sequence

<400> 81

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Ser Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 82

<211> 454

<212> PRT

<213>artificial sequence

<400> 82

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Thr Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 83

<211> 454

<212> PRT

<213>artificial sequence

<400> 83

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Val Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 84

<211> 454

<212> PRT

<213>artificial sequence

<400> 84

Met Ala Ser Asn Ala Ala Ala Ala Ala Ala Lys Ala Glu Glu Ile Val

1 5 10 15

Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro Gly Ser

20 25 30

Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser Glu Gly

35 40 45

Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His Tyr Met

50 55 60

Leu Glu Ala Leu Lys Ala Leu Gly Leu Ser Val Glu Ala Asp Lys Val

65 70 75 80

Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Lys Phe Pro Val Glu

85 90 95

Lys Asp Ala Lys Glu Glu Val Gln Leu Phe Leu Gly Asn Ala Gly Thr

100 105 110

Ala Met Arg Pro Leu Thr Ala Ala Val Thr Ala Ala Gly Gly Asn Ala

115 120 125

Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly

130 135 140

Asp Leu Val Val Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe

145 150 155 160

Leu Gly Thr Glu Cys Pro Pro Val Arg Val Lys Gly Ile Gly Gly Leu

165 170 175

Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln Tyr Leu

180 185 190

Ser Ala Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile

195 200 205

Glu Ile Ile Asp Lys Tyr Ile Ser Ile Pro Tyr Val Glu Met Thr Leu

210 215 220

Arg Leu Met Glu Arg Phe Gly Val Lys Ala Glu His Ser Asp Ser Trp

225 230 235 240

Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn

245 250 255

Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly

260 265 270

Ala Ala Ile Thr Gly Gly Thr Val Thr Val Gln Gly Cys Gly Thr Thr

275 280 285

Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met Met Gly

290 295 300

Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly Pro Pro

305 310 315 320

Arg Glu Pro Tyr Gly Lys Lys His Leu Lys Ala Val Asp Val Asn Met

325 330 335

Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe

340 345 350

Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg Val Lys

355 360 365

Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys Leu Gly

370 375 380

Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro Pro Glu

385 390 395 400

Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala

405 410 415

Met Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Arg

420 425 430

Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp Val Leu

435 440 445

Ser Thr Phe Val Arg Asn

450

<210> 85

<211> 456

<212> PRT

<213>artificial sequence

<400> 85

Met Ala Thr Ser Val Ala Ala Pro Ala Ala Pro Ala Gly Ala Glu Glu

1 5 10 15

Val Val Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro

20 25 30

Gly Ser Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser

35 40 45

Glu Gly Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His

50 55 60

Tyr Met Leu Glu Ala Leu Glu Ala Leu Gly Leu Ser Val Glu Ala Asp

65 70 75 80

Lys Val Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Arg Phe Pro

85 90 95

Val Glu Lys Asp Ala Lys Glu Glu Val Lys Leu Phe Leu Gly Asn Ala

100 105 110

Gly Thr Ala Met Arg Pro Leu Thr Ala Ala Val Val Ala Ala Gly Gly

115 120 125

Asn Ala Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro

130 135 140

Ile Gly Asp Leu Val Val Gly Leu Gln Gln Leu Gly Ala Asp Val Asp

145 150 155 160

Cys Phe Leu Gly Thr Asn Cys Pro Pro Val Arg Ile Asn Gly Lys Gly

165 170 175

Gly Leu Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln

180 185 190

Tyr Leu Ser Ser Leu Leu Met Ala Ala Pro Leu Ala Leu Glu Asp Val

195 200 205

Glu Ile Glu Ile Ile Asp Lys Ala Ile Ser Val Pro Tyr Val Glu Met

210 215 220

Thr Leu Lys Leu Met Glu His Phe Gly Val Thr Ala Glu His Ser Asp

225 230 235 240

Ser Trp Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro

245 250 255

Gly Asn Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu

260 265 270

Ala Gly Ala Ala Ile Thr Gly Gly Thr Val Thr Val Glu Gly Cys Gly

275 280 285

Thr Thr Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met

290 295 300

Met Gly Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly

305 310 315 320

Pro Pro Arg Gln Pro Phe Gly Arg Lys His Leu Lys Ala Val Asp Val

325 330 335

Asn Met Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala

340 345 350

Leu Phe Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg

355 360 365

Val Lys Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys

370 375 380

Leu Gly Ala Thr Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro

385 390 395 400

Pro Glu Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg

405 410 415

Met Ala Met Ala Phe Ser Leu Ala Ala Cys Ala Glu Val Pro Val Thr

420 425 430

Ile Arg Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp

435 440 445

Val Leu Ser Thr Phe Val Lys Asn

450 455

<210> 86

<211> 456

<212> PRT

<213>artificial sequence

<400> 86

Met Ala Thr Ser Val Ala Ala Pro Ala Ala Pro Ala Gly Ala Glu Glu

1 5 10 15

Val Val Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro

20 25 30

Gly Ser Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser

35 40 45

Glu Gly Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His

50 55 60

Tyr Met Leu Glu Ala Leu Glu Ala Leu Gly Leu Ser Val Glu Ala Asp

65 70 75 80

Lys Val Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Arg Phe Pro

85 90 95

Val Glu Lys Asp Ala Lys Glu Glu Val Lys Leu Phe Leu Gly Asn Ala

100 105 110

Gly Thr Ala Met Arg Pro Leu Thr Ala Ala Val Val Ala Ala Gly Gly

115 120 125

Asn Ala Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro

130 135 140

Ile Gly Asp Leu Val Val Gly Leu Gln Gln Leu Gly Ala Asp Val Asp

145 150 155 160

Cys Phe Leu Gly Thr Asn Cys Pro Pro Val Arg Ile Asn Gly Lys Gly

165 170 175

Gly Leu Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln

180 185 190

Tyr Leu Ser Ser Leu Leu Met Ala Ala Pro Leu Ala Leu Glu Asp Val

195 200 205

Glu Ile Glu Ile Ile Asp Lys Gly Ile Ser Val Pro Tyr Val Glu Met

210 215 220

Thr Leu Lys Leu Met Glu His Phe Gly Val Thr Ala Glu His Ser Asp

225 230 235 240

Ser Trp Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro

245 250 255

Gly Asn Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu

260 265 270

Ala Gly Ala Ala Ile Thr Gly Gly Thr Val Thr Val Glu Gly Cys Gly

275 280 285

Thr Thr Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met

290 295 300

Met Gly Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly

305 310 315 320

Pro Pro Arg Gln Pro Phe Gly Arg Lys His Leu Lys Ala Val Asp Val

325 330 335

Asn Met Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala

340 345 350

Leu Phe Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg

355 360 365

Val Lys Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys

370 375 380

Leu Gly Ala Thr Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro

385 390 395 400

Pro Glu Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg

405 410 415

Met Ala Met Ala Phe Ser Leu Ala Ala Cys Ala Glu Val Pro Val Thr

420 425 430

Ile Arg Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp

435 440 445

Val Leu Ser Thr Phe Val Lys Asn

450 455

<210> 87

<211> 456

<212> PRT

<213>artificial sequence

<400> 87

Met Ala Thr Ser Val Ala Ala Pro Ala Ala Pro Ala Gly Ala Glu Glu

1 5 10 15

Val Val Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro

20 25 30

Gly Ser Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser

35 40 45

Glu Gly Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His

50 55 60

Tyr Met Leu Glu Ala Leu Glu Ala Leu Gly Leu Ser Val Glu Ala Asp

65 70 75 80

Lys Val Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Arg Phe Pro

85 90 95

Val Glu Lys Asp Ala Lys Glu Glu Val Lys Leu Phe Leu Gly Asn Ala

100 105 110

Gly Thr Ala Met Arg Pro Leu Thr Ala Ala Val Val Ala Ala Gly Gly

115 120 125

Asn Ala Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro

130 135 140

Ile Gly Asp Leu Val Val Gly Leu Gln Gln Leu Gly Ala Asp Val Asp

145 150 155 160

Cys Phe Leu Gly Thr Asn Cys Pro Pro Val Arg Ile Asn Gly Lys Gly

165 170 175

Gly Leu Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln

180 185 190

Tyr Leu Ser Ser Leu Leu Met Ala Ala Pro Leu Ala Leu Glu Asp Val

195 200 205

Glu Ile Glu Ile Ile Asp Lys Met Ile Ser Val Pro Tyr Val Glu Met

210 215 220

Thr Leu Lys Leu Met Glu His Phe Gly Val Thr Ala Glu His Ser Asp

225 230 235 240

Ser Trp Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro

245 250 255

Gly Asn Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu

260 265 270

Ala Gly Ala Ala Ile Thr Gly Gly Thr Val Thr Val Glu Gly Cys Gly

275 280 285

Thr Thr Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met

290 295 300

Met Gly Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly

305 310 315 320

Pro Pro Arg Gln Pro Phe Gly Arg Lys His Leu Lys Ala Val Asp Val

325 330 335

Asn Met Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala

340 345 350

Leu Phe Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg

355 360 365

Val Lys Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys

370 375 380

Leu Gly Ala Thr Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro

385 390 395 400

Pro Glu Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg

405 410 415

Met Ala Met Ala Phe Ser Leu Ala Ala Cys Ala Glu Val Pro Val Thr

420 425 430

Ile Arg Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp

435 440 445

Val Leu Ser Thr Phe Val Lys Asn

450 455

<210> 88

<211> 456

<212> PRT

<213>artificial sequence

<400> 88

Met Ala Thr Ser Val Ala Ala Pro Ala Ala Pro Ala Gly Ala Glu Glu

1 5 10 15

Val Val Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro

20 25 30

Gly Ser Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser

35 40 45

Glu Gly Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His

50 55 60

Tyr Met Leu Glu Ala Leu Glu Ala Leu Gly Leu Ser Val Glu Ala Asp

65 70 75 80

Lys Val Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Arg Phe Pro

85 90 95

Val Glu Lys Asp Ala Lys Glu Glu Val Lys Leu Phe Leu Gly Asn Ala

100 105 110

Gly Thr Ala Met Arg Pro Leu Thr Ala Ala Val Val Ala Ala Gly Gly

115 120 125

Asn Ala Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro

130 135 140

Ile Gly Asp Leu Val Val Gly Leu Gln Gln Leu Gly Ala Asp Val Asp

145 150 155 160

Cys Phe Leu Gly Thr Asn Cys Pro Pro Val Arg Ile Asn Gly Lys Gly

165 170 175

Gly Leu Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln

180 185 190

Tyr Leu Ser Ser Leu Leu Met Ala Ala Pro Leu Ala Leu Glu Asp Val

195 200 205

Glu Ile Glu Ile Ile Asp Lys Pro Ile Ser Val Pro Tyr Val Glu Met

210 215 220

Thr Leu Lys Leu Met Glu His Phe Gly Val Thr Ala Glu His Ser Asp

225 230 235 240

Ser Trp Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro

245 250 255

Gly Asn Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu

260 265 270

Ala Gly Ala Ala Ile Thr Gly Gly Thr Val Thr Val Glu Gly Cys Gly

275 280 285

Thr Thr Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met

290 295 300

Met Gly Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly

305 310 315 320

Pro Pro Arg Gln Pro Phe Gly Arg Lys His Leu Lys Ala Val Asp Val

325 330 335

Asn Met Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala

340 345 350

Leu Phe Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg

355 360 365

Val Lys Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys

370 375 380

Leu Gly Ala Thr Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro

385 390 395 400

Pro Glu Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg

405 410 415

Met Ala Met Ala Phe Ser Leu Ala Ala Cys Ala Glu Val Pro Val Thr

420 425 430

Ile Arg Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp

435 440 445

Val Leu Ser Thr Phe Val Lys Asn

450 455

<210> 89

<211> 456

<212> PRT

<213>artificial sequence

<400> 89

Met Ala Thr Ser Val Ala Ala Pro Ala Ala Pro Ala Gly Ala Glu Glu

1 5 10 15

Val Val Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro

20 25 30

Gly Ser Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser

35 40 45

Glu Gly Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His

50 55 60

Tyr Met Leu Glu Ala Leu Glu Ala Leu Gly Leu Ser Val Glu Ala Asp

65 70 75 80

Lys Val Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Arg Phe Pro

85 90 95

Val Glu Lys Asp Ala Lys Glu Glu Val Lys Leu Phe Leu Gly Asn Ala

100 105 110

Gly Thr Ala Met Arg Pro Leu Thr Ala Ala Val Val Ala Ala Gly Gly

115 120 125

Asn Ala Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro

130 135 140

Ile Gly Asp Leu Val Val Gly Leu Gln Gln Leu Gly Ala Asp Val Asp

145 150 155 160

Cys Phe Leu Gly Thr Asn Cys Pro Pro Val Arg Ile Asn Gly Lys Gly

165 170 175

Gly Leu Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln

180 185 190

Tyr Leu Ser Ser Leu Leu Met Ala Ala Pro Leu Ala Leu Glu Asp Val

195 200 205

Glu Ile Glu Ile Ile Asp Lys Gln Ile Ser Val Pro Tyr Val Glu Met

210 215 220

Thr Leu Lys Leu Met Glu His Phe Gly Val Thr Ala Glu His Ser Asp

225 230 235 240

Ser Trp Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro

245 250 255

Gly Asn Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu

260 265 270

Ala Gly Ala Ala Ile Thr Gly Gly Thr Val Thr Val Glu Gly Cys Gly

275 280 285

Thr Thr Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met

290 295 300

Met Gly Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly

305 310 315 320

Pro Pro Arg Gln Pro Phe Gly Arg Lys His Leu Lys Ala Val Asp Val

325 330 335

Asn Met Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala

340 345 350

Leu Phe Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg

355 360 365

Val Lys Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys

370 375 380

Leu Gly Ala Thr Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro

385 390 395 400

Pro Glu Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg

405 410 415

Met Ala Met Ala Phe Ser Leu Ala Ala Cys Ala Glu Val Pro Val Thr

420 425 430

Ile Arg Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp

435 440 445

Val Leu Ser Thr Phe Val Lys Asn

450 455

<210> 90

<211> 456

<212> PRT

<213>artificial sequence

<400> 90

Met Ala Thr Ser Val Ala Ala Pro Ala Ala Pro Ala Gly Ala Glu Glu

1 5 10 15

Val Val Leu Gln Pro Ile Arg Glu Ile Ser Gly Ala Val Gln Leu Pro

20 25 30

Gly Ser Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ser Ala Leu Ser

35 40 45

Glu Gly Thr Thr Val Val Asp Asn Leu Leu Asn Ser Glu Asp Val His

50 55 60

Tyr Met Leu Glu Ala Leu Glu Ala Leu Gly Leu Ser Val Glu Ala Asp

65 70 75 80

Lys Val Ala Lys Arg Ala Val Val Val Gly Cys Gly Gly Arg Phe Pro

85 90 95

Val Glu Lys Asp Ala Lys Glu Glu Val Lys Leu Phe Leu Gly Asn Ala

100 105 110

Gly Thr Ala Met Arg Pro Leu Thr Ala Ala Val Val Ala Ala Gly Gly

115 120 125

Asn Ala Thr Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro

130 135 140

Ile Gly Asp Leu Val Val Gly Leu Gln Gln Leu Gly Ala Asp Val Asp

145 150 155 160

Cys Phe Leu Gly Thr Asn Cys Pro Pro Val Arg Ile Asn Gly Lys Gly

165 170 175

Gly Leu Pro Gly Gly Lys Val Lys Leu Ser Gly Ser Ile Ser Ser Gln

180 185 190

Tyr Leu Ser Ser Leu Leu Met Ala Ala Pro Leu Ala Leu Glu Asp Val

195 200 205

Glu Ile Glu Ile Ile Asp Lys Ser Ile Ser Val Pro Tyr Val Glu Met

210 215 220

Thr Leu Lys Leu Met Glu His Phe Gly Val Thr Ala Glu His Ser Asp

225 230 235 240

Ser Trp Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys Tyr Lys Ser Pro

245 250 255

Gly Asn Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu

260 265 270

Ala Gly Ala Ala Ile Thr Gly Gly Thr Val Thr Val Glu Gly Cys Gly

275 280 285

Thr Thr Ser Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Met

290 295 300

Met Gly Ala Lys Val Thr Trp Thr Asp Thr Ser Val Thr Val Thr Gly

305 310 315 320

Pro Pro Arg Gln Pro Phe Gly Arg Lys His Leu Lys Ala Val Asp Val

325 330 335

Asn Met Asn Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala

340 345 350

Leu Phe Ala Asp Gly Pro Thr Ala Ile Arg Asp Val Ala Ser Trp Arg

355 360 365

Val Lys Glu Thr Glu Arg Met Val Ala Ile Arg Thr Glu Leu Thr Lys

370 375 380

Leu Gly Ala Thr Val Glu Glu Gly Pro Asp Tyr Cys Ile Ile Thr Pro

385 390 395 400

Pro Glu Lys Leu Asn Ile Thr Ala Ile Asp Thr Tyr Asp Asp His Arg

405 410 415

Met Ala Met Ala Phe Ser Leu Ala Ala Cys Ala Glu Val Pro Val Thr

420 425 430

Ile Arg Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro Asn Tyr Phe Asp

435 440 445

Val Leu Ser Thr Phe Val Lys Asn

450 455

<210> 91

<211> 452

<212> PRT

<213>artificial sequence

<400> 91

Met Ala Ala Ala Glu Lys Pro Ser Thr Ala Pro Glu Ile Val Leu Glu

1 5 10 15

Pro Ile Lys Asp Ile Ser Gly Thr Ile Thr Leu Pro Gly Ser Lys Ser

20 25 30

Leu Ser Asn Arg Ile Leu Leu Leu Ala Ala Leu Ser Glu Gly Thr Thr

35 40 45

Val Val Asp Asn Leu Leu Tyr Ser Glu Asp Ile His Tyr Met Leu Gly

50 55 60

Ala Leu Arg Thr Leu Gly Leu Arg Val Glu Asp Asp Gln Thr Thr Lys

65 70 75 80

Gln Ala Ile Val Glu Gly Cys Gly Gly Leu Phe Pro Thr Ile Lys Glu

85 90 95

Ser Lys Asp Glu Ile Asn Leu Phe Leu Gly Asn Ala Gly Thr Ala Met

100 105 110

Arg Pro Leu Thr Ala Ala Val Val Ala Ala Gly Gly Asn Ala Ser Tyr

115 120 125

Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly Asp Leu

130 135 140

Val Ala Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe Leu Gly

145 150 155 160

Thr Asn Cys Pro Pro Val Arg Val Asn Gly Lys Gly Gly Leu Pro Gly

165 170 175

Gly Lys Val Lys Leu Ser Gly Ser Val Ser Ser Gln Tyr Leu Thr Ala

180 185 190

Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile Glu Ile

195 200 205

Val Asp Lys Pro Ile Ser Val Pro Tyr Val Glu Met Thr Leu Lys Leu

210 215 220

Met Glu Arg Phe Gly Val Ser Val Glu His Ser Gly Asp Trp Asp Lys

225 230 235 240

Phe Leu Val His Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn Ala Phe

245 250 255

Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly Ala Ala

260 265 270

Val Thr Gly Gly Thr Ile Thr Val Asn Gly Cys Gly Thr Ser Ser Leu

275 280 285

Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Lys Met Gly Ala Lys

290 295 300

Val Thr Trp Ser Glu Asn Ser Val Thr Val Thr Gly Pro Pro Gln Asp

305 310 315 320

Ser Ser Gly Gln Lys Val Leu Gln Gly Ile Asp Val Asn Met Asn Lys

325 330 335

Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe Ala Asn

340 345 350

Gly Gln Thr Thr Ile Arg Asp Val Ala Ser Trp Arg Val Lys Glu Thr

355 360 365

Glu Arg Met Ile Ala Ile Cys Thr Glu Leu Arg Lys Leu Gly Ala Thr

370 375 380

Val Glu Glu Gly Pro Asp Tyr Cys Val Ile Thr Pro Pro Glu Lys Leu

385 390 395 400

Asn Val Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala Met Ala

405 410 415

Phe Ser Leu Ala Ala Cys Gly Asp Val Pro Val Thr Ile Lys Asp Pro

420 425 430

Gly Cys Thr Arg Lys Thr Phe Pro Asp Tyr Phe Glu Val Leu Glu Arg

435 440 445

Phe Thr Arg His

450

<210> 92

<211> 452

<212> PRT

<213>artificial sequence

<400> 92

Met Ala Ala Ala Glu Lys Pro Ser Thr Ala Pro Glu Ile Val Leu Glu

1 5 10 15

Pro Ile Lys Asp Ile Ser Gly Thr Ile Thr Leu Pro Gly Ser Lys Ser

20 25 30

Leu Ser Asn Arg Ile Leu Leu Leu Ala Ala Leu Ser Glu Gly Thr Thr

35 40 45

Val Val Asp Asn Leu Leu Tyr Ser Glu Asp Ile His Tyr Met Leu Gly

50 55 60

Ala Leu Arg Thr Leu Gly Leu Arg Val Glu Asp Asp Gln Thr Thr Lys

65 70 75 80

Gln Ala Ile Val Glu Gly Cys Gly Gly Leu Phe Pro Thr Ile Lys Glu

85 90 95

Ser Lys Asp Glu Ile Asn Leu Phe Leu Gly Asn Ala Gly Thr Ala Met

100 105 110

Arg Pro Leu Thr Ala Ala Val Val Ala Ala Gly Gly Asn Ala Ser Tyr

115 120 125

Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly Asp Leu

130 135 140

Val Ala Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe Leu Gly

145 150 155 160

Thr Asn Cys Pro Pro Val Arg Val Asn Gly Lys Gly Gly Leu Pro Gly

165 170 175

Gly Lys Val Lys Leu Ser Gly Ser Val Ser Ser Gln Tyr Leu Thr Ala

180 185 190

Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile Glu Ile

195 200 205

Val Asp Lys Gln Ile Ser Val Pro Tyr Val Glu Met Thr Leu Lys Leu

210 215 220

Met Glu Arg Phe Gly Val Ser Val Glu His Ser Gly Asp Trp Asp Lys

225 230 235 240

Phe Leu Val His Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn Ala Phe

245 250 255

Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly Ala Ala

260 265 270

Val Thr Gly Gly Thr Ile Thr Val Asn Gly Cys Gly Thr Ser Ser Leu

275 280 285

Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Lys Met Gly Ala Lys

290 295 300

Val Thr Trp Ser Glu Asn Ser Val Thr Val Thr Gly Pro Pro Gln Asp

305 310 315 320

Ser Ser Gly Gln Lys Val Leu Gln Gly Ile Asp Val Asn Met Asn Lys

325 330 335

Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe Ala Asn

340 345 350

Gly Gln Thr Thr Ile Arg Asp Val Ala Ser Trp Arg Val Lys Glu Thr

355 360 365

Glu Arg Met Ile Ala Ile Cys Thr Glu Leu Arg Lys Leu Gly Ala Thr

370 375 380

Val Glu Glu Gly Pro Asp Tyr Cys Val Ile Thr Pro Pro Glu Lys Leu

385 390 395 400

Asn Val Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala Met Ala

405 410 415

Phe Ser Leu Ala Ala Cys Gly Asp Val Pro Val Thr Ile Lys Asp Pro

420 425 430

Gly Cys Thr Arg Lys Thr Phe Pro Asp Tyr Phe Glu Val Leu Glu Arg

435 440 445

Phe Thr Arg His

450

<210> 93

<211> 452

<212> PRT

<213>artificial sequence

<400> 93

Met Ala Ala Ala Glu Lys Pro Ser Thr Ala Pro Glu Ile Val Leu Glu

1 5 10 15

Pro Ile Lys Asp Ile Ser Gly Thr Ile Thr Leu Pro Gly Ser Lys Ser

20 25 30

Leu Ser Asn Arg Ile Leu Leu Leu Ala Ala Leu Ser Glu Gly Thr Thr

35 40 45

Val Val Asp Asn Leu Leu Tyr Ser Glu Asp Ile His Tyr Met Leu Gly

50 55 60

Ala Leu Arg Thr Leu Gly Leu Arg Val Glu Asp Asp Gln Thr Thr Lys

65 70 75 80

Gln Ala Ile Val Glu Gly Cys Gly Gly Leu Phe Pro Thr Ile Lys Glu

85 90 95

Ser Lys Asp Glu Ile Asn Leu Phe Leu Gly Asn Ala Ala Thr Ala Met

100 105 110

Arg Pro Leu Thr Ala Ala Val Val Ala Ala Gly Gly Asn Ala Ser Tyr

115 120 125

Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly Asp Leu

130 135 140

Val Ala Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Phe Leu Gly

145 150 155 160

Thr Asn Cys Pro Pro Val Arg Val Asn Gly Lys Gly Gly Leu Pro Gly

165 170 175

Gly Lys Val Lys Leu Ser Gly Ser Val Ser Ser Gln Tyr Leu Thr Ala

180 185 190

Leu Leu Met Ala Ala Pro Leu Ala Leu Gly Asp Val Glu Ile Glu Ile

195 200 205

Val Asp Lys Met Ile Ser Val Pro Tyr Val Glu Met Thr Leu Lys Leu

210 215 220

Met Glu Arg Phe Gly Val Ser Val Glu His Ser Gly Asp Trp Asp Lys

225 230 235 240

Phe Leu Val His Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn Ala Phe

245 250 255

Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Gly Ala Ala

260 265 270

Val Thr Gly Gly Thr Ile Thr Val Asn Gly Cys Gly Thr Ser Ser Leu

275 280 285

Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Lys Met Gly Ala Lys

290 295 300

Val Thr Trp Ser Glu Asn Ser Val Thr Val Thr Gly Pro Pro Gln Asp

305 310 315 320

Ser Ser Gly Gln Lys Val Leu Gln Gly Ile Asp Val Asn Met Asn Lys

325 330 335

Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Phe Ala Asn

340 345 350

Gly Gln Thr Thr Ile Arg Asp Val Ala Ser Trp Arg Val Lys Glu Thr

355 360 365

Glu Arg Met Ile Ala Ile Cys Thr Glu Leu Arg Lys Leu Gly Ala Thr

370 375 380

Val Glu Glu Gly Pro Asp Tyr Cys Val Ile Thr Pro Pro Glu Lys Leu

385 390 395 400

Asn Val Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala Met Ala

405 410 415

Phe Ser Leu Ala Ala Cys Gly Asp Val Pro Val Thr Ile Lys Asp Pro

420 425 430

Gly Cys Thr Arg Lys Thr Phe Pro Asp Tyr Phe Glu Val Leu Glu Arg

435 440 445

Phe Thr Arg His

450

Claims (10)

1. a kind of plant EPSPS mutant, which is characterized in that it is compared with Escherichia coli EPSPS, the plant EPSPS mutation The amino acid sequence of body corresponds to the 138th of Escherichia coli EPSPS with mutation A38X₁And/or the 195th have mutation L195X₂, wherein X₁=C, D, E, I, L, M, N or S, X₂=A, C, E, F, G, H, K, M, N, Q, R, S, T, V or Y.

2. plant EPSPS mutant according to claim 1, which is characterized in that the plant EPSPS mutant from Wheat, rice, barley, oat, corn, sorghum, millet, buckwheat, maiden cane, mung bean, semen viciae fabae, pea, hyacinth bean, sweet potato, potato, Cotton, soybean, rape, sesame, peanut, sunflower, radish, carrot, turnip, beet, Chinese cabbage, leaf mustard, wild cabbage, cauliflower, Cabbage mustard, cucumber, cucurbita pepo, pumpkin, wax gourd, balsam pear, sponge gourd, snake melon, watermelon, muskmelon, tomato, eggplant, capsicum, Kidney bean, cowpea, Green soy bean, leek, shallot, onion, leek, spinach, celery, three-coloured amaranth, lettuce, crowndaisy chrysanthemum, day lily, grape, strawberry, beet, sugarcane, Any one plant in tobacco, clover, herbage, turfgrass, tea and cassava.

3. a kind of encoding gene, which is characterized in that it encodes plant EPSPS mutant of any of claims 1-2.

4. containing the carrier of encoding gene as claimed in claim 3.

5. containing the recombinant bacterium or recombinant cell of carrier as claimed in claim 4.

6. described in plant EPSPS mutant of any of claims 1 or 2, encoding gene as claimed in claim 3, claim 4 Carrier or claim 5 described in recombinant bacterium or recombinant cell cultivating the application in anti-glyphosate plants.

7. application according to claim 6, characterized in that it comprises: carrier is converted purpose plant, the carrier contains There is the encoding gene for encoding the plant EPSPS mutant.

8. application according to claim 6, characterized in that it comprises: the endogenous EPSPS gene of modification purpose plant, It is set to encode the plant EPSPS mutant.

9. application according to claim 6, characterized in that it comprises: to plant cell, tissue, individual or group into Row mutagenesis, screening, make it encode the plant EPSPS mutant.

10. according to the described in any item applications of claim 6-9, which is characterized in that plant is selected from wheat, rice, barley, swallow Wheat, corn, sorghum, millet, buckwheat, maiden cane, mung bean, semen viciae fabae, pea, hyacinth bean, sweet potato, potato, cotton, soybean, rape, sesame Fiber crops, peanut, sunflower, radish, carrot, turnip, beet, Chinese cabbage, leaf mustard, wild cabbage, cauliflower, cabbage mustard, cucumber, cucurbita pepo, Pumpkin, wax gourd, balsam pear, sponge gourd, snake melon, watermelon, muskmelon, tomato, eggplant, capsicum, Kidney bean, cowpea, green soy bean, leek, shallot, ocean Green onion, leek, spinach, celery, three-coloured amaranth, lettuce, crowndaisy chrysanthemum, day lily, grape, strawberry, beet, sugarcane, tobacco, clover, herbage, grass Any one in level ground grass, tea and cassava.