CN109549963A - A kind of production method of the prepared slices of Chinese crude drugs - Google Patents
A kind of production method of the prepared slices of Chinese crude drugs Download PDFInfo
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- CN109549963A CN109549963A CN201910034263.7A CN201910034263A CN109549963A CN 109549963 A CN109549963 A CN 109549963A CN 201910034263 A CN201910034263 A CN 201910034263A CN 109549963 A CN109549963 A CN 109549963A
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Abstract
The present invention provides a kind of production methods of prepared slices of Chinese crude drugs, the specific steps are as follows: (1) after fresh Rhizoma Atractylodis Macrocephalae robs washing, slice obtains Rhizoma Atractylodis Macrocephalae slice, spare；(2) it prepares seed liquor: respectively obtaining respective fermentation liquid after aspergillus oryzae and trichoderma harzianum are individually fermented, then mix, obtain seed mixture liquid；(3) slice of Rhizoma Atractylodis Macrocephalae obtained by step (1) is soaked in culture solution, seed mixture liquid obtained by step (2) is then added, fermented and cultured is taken out, the Rhizoma Atractylodis Macrocephalae that must ferment slice；(4) fermentation Rhizoma Atractylodis Macrocephalae slice obtained by step (3) is 3~4 hours stifling in 80~90 DEG C using the nitration mixture aqueous solution of lactic acid and acetic acid, it is taken out rapidly after immersing the ethanol solution of stannous chloride, then 300~400W microwave treatment 5~10 minutes must activate Rhizoma Atractylodis Macrocephalae slice；(5) activation Rhizoma Atractylodis Macrocephalae slice obtained by step (4) is crushed to the Rhizoma Atractylodis Macrocephalae powder of 0.5~1mm of partial size, is then handled using steam explosion technology, then ultra micro nanometer pulverization.
Description
Technical field
The present invention relates to technical field of traditional Chinese medicines, particularly, are related to a kind of production method of prepared slices of Chinese crude drugs.
Background technique
Traditional Chinese medicine has very long history, and Chinese medicine plays very important work in preventing and treating many diseases
With, but Chinese medicine is relatively slow in modern times development, this is because the physics and differing chemical properties of Chinese medicine, complicated component,
There are many principles unclear.
The prepared slices of Chinese crude drugs are the Chinese medicine that Chinese medicine can be directly used for tcm clinical practice after Processing methods, present medicine materical crude slice type
It is only limitted to the piece of Chinese medicine, section, block, powder, cake, glue, extracts seven class of granule, Chinese medicine Preparation process skill far can not be adapted to
The needs of art.Importantly, existing medicine materical crude slice type flaw is obvious, and such as: quality discrepancy is big, it is difficult to reach and homogenize；Separately
Outside, in medicine materical crude slice process, it often will cause active ingredients from traditional Chinese medicinal loss, Chinese medicine caused to waste.
Widely different at being grouped as due to Chinese medicine, the physicochemical properties of effective component are different, in different
Medicinal material, corresponding medicine materical crude slice production method should all be adjusted, be must not make sweeping generalizations.Rhizoma Atractylodis Macrocephalae is Chinese medicine very common simply
Material is used as medicine with rhizome, is had strengthening the spleen and replenishing qi, eliminate dampness and have diuretic effect, hidroschesis and miscarriage prevention and other effects, is mainly used for spleen eating less, abdomen
Swollen diarrhea, phlegm retention anti-dazzle nervous, oedema, spontaneous perspiration, threatened abortion.Traditional concocting method of Rhizoma Atractylodis Macrocephalae has following three kinds:
1, raw Rhizoma Atractylodis Macrocephalae: net impurity is picked, is soaked in water, soaking time should be appropriate according to season, climate change and Rhizoma Atractylodis Macrocephalae size
It grasps, pulls out, moisturize after bubble, be sliced, dry；
2, wheat bran: being first spread in hot pot by rhizoma atractylodis macrocephalae, and when cigarette is emerged, bighead atractylodes rhizome piece is poured into micro- fry to faint yellow, taking-up, sieve
It goes after wheat bran to cool (100 jin of every bighead atractylodes rhizome piece, with wheat bran 10 jin)；
3. Rhizoma Atractylodis Macrocephalae preparata: bighead atractylodes rhizome piece being set and is fried with high heat in pot to coke yellow, clear water is sprayed, taking-up is dried；
4, stir-baked RHIZOMA ATRACTYLODIS MACROCEPHALAE with soil: taking humus flava usta fine powder, set in pot and make popular, and bighead atractylodes rhizome piece is added, and fries taking-up when hanging with ashen to outside,
Soil is weeded out, is cooled (100 jin of every bighead atractylodes rhizome piece, with humus flava usta fine powder 20 jin).
Modern research shows that these traditional concocting methods can destroy many effective components, and decocting in the later period
It cannot achieve being fully dissolved out for effective component in journey, undoubtedly cause Chinese medicine loss.
Summary of the invention
It is an object of that present invention to provide a kind of production method of prepared slices of Chinese crude drugs, to solve, quality discrepancy is big, effective component damage
The technical problems such as mistake.
To achieve the above object, the present invention provides a kind of production methods of prepared slices of Chinese crude drugs, the specific steps are as follows:
(1) after fresh Rhizoma Atractylodis Macrocephalae robs washing, slice obtains Rhizoma Atractylodis Macrocephalae slice, spare；
(2) it prepares seed liquor: respectively obtaining respective fermentation liquid after aspergillus oryzae and trichoderma harzianum are individually fermented, then
Mixing, obtains seed mixture liquid；
(3) slice of Rhizoma Atractylodis Macrocephalae obtained by step (1) is soaked in culture solution, seed mixture liquid obtained by step (2) is then added,
Fermented and cultured is taken out, the Rhizoma Atractylodis Macrocephalae that must ferment slice；
(4) fermentation Rhizoma Atractylodis Macrocephalae slice obtained by step (3) using the nitration mixture aqueous solution of lactic acid and acetic acid in 80~90 DEG C stifling 3~
It 4 hours, takes out rapidly, then 300~400W microwave treatment 5~10 minutes, must activate after immersing the ethanol solution of stannous chloride
Rhizoma Atractylodis Macrocephalae slice；
(5) activation Rhizoma Atractylodis Macrocephalae slice obtained by step (4) is crushed to the Rhizoma Atractylodis Macrocephalae powder of 0.5~1mm of partial size, it is then quick-fried using steam
Broken technology is handled, then ultra micro nanometer pulverization.
Preferably, in step (1), slice thickness is 2~5mm.
Preferably, in step (2), in seed mixture liquid, the content of aspergillus oryzae and trichoderma harzianum is respectively 30~4,000,000,000/
G, 15~2,500,000,000/g.
Preferably, in step (2), the fermentation condition of aspergillus oryzae are as follows: Czapek's medium, 30~35 DEG C of fermentation temperature, fermentation
Time 24~48 hours；The fermentation condition of trichoderma harzianum are as follows: Czapek's medium, 35~40 DEG C of fermentation temperature, fermentation time 24
~48 hours.
Preferably, in step (3), by weight percentage, the composition of the culture solution is as follows: peptone 2~3%, nitre
Sour sodium 1.2~1.5%, calcium chloride 1~1.2%, ammonium sulfate 0.8~1%, manganese sulfate 0.6~0.7%, urea 0.5~0.7%,
Surplus is distilled water.
Preferably, in step (3), Rhizoma Atractylodis Macrocephalae slice, culture solution, seed liquor mass volume ratio be 1:20~30mL:8~
10mL。
Preferably, in step (3), the specific method of fermented and cultured is: pH=6~7,40~50 DEG C of temperature, 80~100r/
Shaken cultivation 58~72 hours minute.
Preferably, in step (4), in the nitration mixture aqueous solution of the lactic acid and acetic acid, the mass percent of lactic acid is 5~
10%, the mass percent of acetic acid is 35~40%.
Preferably, in step (4), the mass concentration of stannous chloride is 5~10% in the ethanol solution of stannous chloride.
Preferably, in step (5), the process conditions of the steam explosion technology are as follows: set Rhizoma Atractylodis Macrocephalae powder loading steam blasting
In standby explosion chamber, pressure is discharged rapidly after being kept for 1~2 minute under the conditions of 1~2.5MPa.
Preferably, in step (5), the process conditions of ultra micro nanometer pulverization are as follows: use airslide disintegrating mill, stream pressure is
1100kPa, charging rate 180r/min, grade frequency 35Hz, grinding time are 60~80 minutes.
The invention has the following advantages:
The present invention provides a kind of prepared slices of Chinese crude drugs production methods for Rhizoma Atractylodis Macrocephalae, and Rhizoma Atractylodis Macrocephalae slice first is made in fresh Rhizoma Atractylodis Macrocephalae,
Then by Rhizoma Atractylodis Macrocephalae slice be soaked in culture solution, be added seed mixture liquid, fermented and cultured must ferment Rhizoma Atractylodis Macrocephalae slice；Fermentation Rhizoma Atractylodis Macrocephalae is cut
After piece microwave treatment, it is sliced using the stifling activation Rhizoma Atractylodis Macrocephalae that obtains of the nitration mixture aqueous solution of lactic acid and acetic acid；Finally activation Rhizoma Atractylodis Macrocephalae slice
Through crushing, steam blasting and ultra micro nanometer pulverization；Remain the effective component in Rhizoma Atractylodis Macrocephalae as far as possible in the production process, and
Uniformity is good, avoids batch quality discrepancy.
Seed mixture liquid is obtained by mixing after individually being fermented by aspergillus oryzae and trichoderma harzianum, in independent fermentation process,
Aspergillus oryzae produces amylase, carbohydrase, cellulase, trichoderma harzianum cellulase-producing, after the two mixing, due to trichoderma harzianum
With infiltrative effect is increased, it can promote aspergillus oryzae and generate more amylase, carbohydrase and cellulase, considerably increase
The yield of cellulase, to realize effective degradation to cell wall cellulose, in conjunction with produced amylase and carbohydrase
Effect promotes Rhizoma Atractylodis Macrocephalae effective component (atractylodes lactone, soluble polysaccharide, amino acid etc.) release, facilitates the abundant benefit of Rhizoma Atractylodis Macrocephalae
With.
Fermentation Rhizoma Atractylodis Macrocephalae slice is activated, including two steps: the nitration mixture aqueous solution of lactic acid and acetic acid is stifling, micro-
Wave processing.In the mixed acid solution of lactic acid and acetic acid, the small acetic acid dosage of lactic acid dosage is big, can destroy under the acid condition of nitration mixture
Enzymatic activity has killed most of residual enzyme in fermentation Rhizoma Atractylodis Macrocephalae slice, has avoided the significantly degradation to Rhizoma Atractylodis Macrocephalae effective component, another
Aspect (is used in the surface that fermentation Rhizoma Atractylodis Macrocephalae is sliced and a small amount of lactic acid of internal importing, subsequent microwave processing process in stannous chloride
Measure very little, will not influence medicinal) catalytic action under, lactic acid polymerize to form polylactic acid, to Rhizoma Atractylodis Macrocephalae effective component formed protective layer,
Effective component is avoided to be lost.Meanwhile microwave action aggravation Rhizoma Atractylodis Macrocephalae internal structure is further decomposed, and then induction atractylodes lactone etc. has
Ingredient separate out is imitated, making full use of for Rhizoma Atractylodis Macrocephalae is realized, and play desiccation, facilitates subsequent crushing.
Last pulverising step includes preliminary crushing, steam blasting and ultra micro nanometer pulverization, due to being activated before
Processing assigns the good mobility of medicine materical crude slice in conjunction with Steam explosion treatment, and long-term preservation is not reunited agglomeration.
Other than objects, features and advantages described above, there are also other objects, features and advantages by the present invention.
The present invention is further detailed explanation below.
Specific embodiment
The embodiment of the present invention is described in detail below, but the present invention can be limited and be covered according to claim
Multitude of different ways implement.
Embodiment 1:
A kind of production method of the prepared slices of Chinese crude drugs, the specific steps are as follows:
(1) after fresh Rhizoma Atractylodis Macrocephalae robs washing, slice obtains Rhizoma Atractylodis Macrocephalae slice, spare；
(2) it prepares seed liquor: respectively obtaining respective fermentation liquid after aspergillus oryzae and trichoderma harzianum are individually fermented, then
Mixing, obtains seed mixture liquid；
(3) slice of Rhizoma Atractylodis Macrocephalae obtained by step (1) is soaked in culture solution, seed mixture liquid obtained by step (2) is then added,
Fermented and cultured is taken out, the Rhizoma Atractylodis Macrocephalae that must ferment slice；
(4) fermentation Rhizoma Atractylodis Macrocephalae slice obtained by step (3) is 3 hours stifling in 80 DEG C using the nitration mixture aqueous solution of lactic acid and acetic acid,
The ethanol solution (mass concentration of stannous chloride is 5%) for immersing stannous chloride takes out rapidly afterwards, and then 300W microwave treatment 5 is divided
Clock must activate Rhizoma Atractylodis Macrocephalae slice；
(5) activation Rhizoma Atractylodis Macrocephalae slice obtained by step (4) is crushed to the Rhizoma Atractylodis Macrocephalae powder of partial size 0.5mm, then uses steam blasting
Technology is handled, then ultra micro nanometer pulverization.
Wherein, in step (1), slice thickness 2mm.
In step (2), in seed mixture liquid, the content of aspergillus oryzae and trichoderma harzianum is respectively 3,000,000,000/g, 1,500,000,000/g.
In step (2), the fermentation condition of aspergillus oryzae are as follows: Czapek's medium, 30 DEG C of fermentation temperature, fermentation time 24 hours；
The fermentation condition of trichoderma harzianum are as follows: Czapek's medium, 35 DEG C of fermentation temperature, fermentation time 24 hours.
In step (3), by weight percentage, the composition of the culture solution is as follows: peptone 2%, sodium nitrate 1.2%,
Calcium chloride 1%, ammonium sulfate 0.8%, manganese sulfate 0.6%, urea 0.5%, surplus are distilled water.
In step (3), Rhizoma Atractylodis Macrocephalae slice, culture solution, seed liquor mass volume ratio be 1:20mL:8mL.
In step (3), the specific method of fermented and cultured is: pH=6,40 DEG C of temperature, 80r/ minutes shaken cultivations 58 hours.
In step (4), in the nitration mixture aqueous solution of the lactic acid and acetic acid, the mass percent of lactic acid is 5%, the matter of acetic acid
Measuring percentage is 35%.
In step (5), the process conditions of the steam explosion technology are as follows: Rhizoma Atractylodis Macrocephalae powder is packed into the explosion of steam blasting equipment
In chamber, pressure is discharged rapidly after being kept for 1 minute under the conditions of 1MPa.
In step (5), the process conditions of ultra micro nanometer pulverization are as follows: use airslide disintegrating mill, stream pressure 1100kPa,
Charging rate is 180r/min, and grade frequency 35Hz, grinding time is 60 minutes.
Embodiment 2:
A kind of production method of the prepared slices of Chinese crude drugs, the specific steps are as follows:
(1) after fresh Rhizoma Atractylodis Macrocephalae robs washing, slice obtains Rhizoma Atractylodis Macrocephalae slice, spare；
(2) it prepares seed liquor: respectively obtaining respective fermentation liquid after aspergillus oryzae and trichoderma harzianum are individually fermented, then
Mixing, obtains seed mixture liquid；
(3) slice of Rhizoma Atractylodis Macrocephalae obtained by step (1) is soaked in culture solution, seed mixture liquid obtained by step (2) is then added,
Fermented and cultured is taken out, the Rhizoma Atractylodis Macrocephalae that must ferment slice；
(4) fermentation Rhizoma Atractylodis Macrocephalae slice obtained by step (3) is 4 hours stifling in 90 DEG C using the nitration mixture aqueous solution of lactic acid and acetic acid,
The ethanol solution (mass concentration of stannous chloride is 10%) for immersing stannous chloride takes out rapidly afterwards, then 400W microwave treatment 10
Minute, Rhizoma Atractylodis Macrocephalae slice must be activated；
(5) activation Rhizoma Atractylodis Macrocephalae slice obtained by step (4) is crushed to the Rhizoma Atractylodis Macrocephalae powder of partial size 1mm, then uses steam blasting skill
Art is handled, then ultra micro nanometer pulverization.
Wherein, in step (1), slice thickness 5mm.
In step (2), in seed mixture liquid, the content of aspergillus oryzae and trichoderma harzianum is respectively 4,000,000,000/g, 2,500,000,000/g.
In step (2), the fermentation condition of aspergillus oryzae are as follows: Czapek's medium, 35 DEG C of fermentation temperature, fermentation time 48 hours；
The fermentation condition of trichoderma harzianum are as follows: Czapek's medium, 40 DEG C of fermentation temperature, fermentation time 48 hours.
In step (3), by weight percentage, the composition of the culture solution is as follows: peptone 3%, sodium nitrate 1.5%,
Calcium chloride 1.2%, ammonium sulfate 1%, manganese sulfate 0.7%, urea 0.7%, surplus are distilled water.
In step (3), Rhizoma Atractylodis Macrocephalae slice, culture solution, seed liquor mass volume ratio be 1:30mL:10mL.
In step (3), the specific method of fermented and cultured is: pH=7, temperature 50 C, shaken cultivation 72 is small within 100r/ minutes
When.
In step (4), in the nitration mixture aqueous solution of the lactic acid and acetic acid, the mass percent of lactic acid is 10%, acetic acid
Mass percent is 40%.
In step (5), the process conditions of the steam explosion technology are as follows: Rhizoma Atractylodis Macrocephalae powder is packed into the explosion of steam blasting equipment
In chamber, pressure is discharged rapidly after being kept for 2 minutes under the conditions of 2.5MPa.
In step (5), the process conditions of ultra micro nanometer pulverization are as follows: use airslide disintegrating mill, stream pressure 1100kPa,
Charging rate is 180r/min, and grade frequency 35Hz, grinding time is 80 minutes.
Embodiment 3:
A kind of production method of the prepared slices of Chinese crude drugs, the specific steps are as follows:
(1) after fresh Rhizoma Atractylodis Macrocephalae robs washing, slice obtains Rhizoma Atractylodis Macrocephalae slice, spare；
(2) it prepares seed liquor: respectively obtaining respective fermentation liquid after aspergillus oryzae and trichoderma harzianum are individually fermented, then
Mixing, obtains seed mixture liquid；
(3) slice of Rhizoma Atractylodis Macrocephalae obtained by step (1) is soaked in culture solution, seed mixture liquid obtained by step (2) is then added,
Fermented and cultured is taken out, the Rhizoma Atractylodis Macrocephalae that must ferment slice；
(4) fermentation Rhizoma Atractylodis Macrocephalae slice obtained by step (3) is 4 hours stifling in 80 DEG C using the nitration mixture aqueous solution of lactic acid and acetic acid,
The ethanol solution (mass concentration of stannous chloride is 5%) for immersing stannous chloride takes out rapidly afterwards, then 300W microwave treatment 10
Minute, Rhizoma Atractylodis Macrocephalae slice must be activated；
(5) activation Rhizoma Atractylodis Macrocephalae slice obtained by step (4) is crushed to the Rhizoma Atractylodis Macrocephalae powder of partial size 0.5mm, then uses steam blasting
Technology is handled, then ultra micro nanometer pulverization.
Wherein, in step (1), slice thickness 2mm.
In step (2), in seed mixture liquid, the content of aspergillus oryzae and trichoderma harzianum is respectively 4,000,000,000/g, 1,500,000,000/g.
In step (2), the fermentation condition of aspergillus oryzae are as follows: Czapek's medium, 35 DEG C of fermentation temperature, fermentation time 24 hours；
The fermentation condition of trichoderma harzianum are as follows: Czapek's medium, 40 DEG C of fermentation temperature, fermentation time 24 hours.
In step (3), by weight percentage, the composition of the culture solution is as follows: peptone 3%, sodium nitrate 1.2%,
Calcium chloride 1.2%, ammonium sulfate 0.8%, manganese sulfate 0.7%, urea 0.5%, surplus are distilled water.
In step (3), Rhizoma Atractylodis Macrocephalae slice, culture solution, seed liquor mass volume ratio be 1:30mL:8mL.
In step (3), the specific method of fermented and cultured is: pH=7, and 40 DEG C of temperature, shaken cultivation 58 is small within 100r/ minutes
When.
In step (4), in the nitration mixture aqueous solution of the lactic acid and acetic acid, the mass percent of lactic acid is 10%, acetic acid
Mass percent is 35%.
In step (5), the process conditions of the steam explosion technology are as follows: Rhizoma Atractylodis Macrocephalae powder is packed into the explosion of steam blasting equipment
In chamber, pressure is discharged rapidly after being kept for 1 minute under the conditions of 2.5MPa.
In step (5), the process conditions of ultra micro nanometer pulverization are as follows: use airslide disintegrating mill, stream pressure 1100kPa,
Charging rate is 180r/min, and grade frequency 35Hz, grinding time is 80 minutes.
Embodiment 4:
A kind of production method of the prepared slices of Chinese crude drugs, the specific steps are as follows:
(1) after fresh Rhizoma Atractylodis Macrocephalae robs washing, slice obtains Rhizoma Atractylodis Macrocephalae slice, spare；
(2) it prepares seed liquor: respectively obtaining respective fermentation liquid after aspergillus oryzae and trichoderma harzianum are individually fermented, then
Mixing, obtains seed mixture liquid；
(3) slice of Rhizoma Atractylodis Macrocephalae obtained by step (1) is soaked in culture solution, seed mixture liquid obtained by step (2) is then added,
Fermented and cultured is taken out, the Rhizoma Atractylodis Macrocephalae that must ferment slice；
(4) fermentation Rhizoma Atractylodis Macrocephalae slice obtained by step (3) is 3 hours stifling in 90 DEG C using the nitration mixture aqueous solution of lactic acid and acetic acid,
The ethanol solution (mass concentration of stannous chloride is 10%) for immersing stannous chloride takes out rapidly afterwards, then 400W microwave treatment 5
Minute, Rhizoma Atractylodis Macrocephalae slice must be activated；
(5) activation Rhizoma Atractylodis Macrocephalae slice obtained by step (4) is crushed to the Rhizoma Atractylodis Macrocephalae powder of partial size 1mm, then uses steam blasting skill
Art is handled, then ultra micro nanometer pulverization.
Wherein, in step (1), slice thickness 5mm.
In step (2), in seed mixture liquid, the content of aspergillus oryzae and trichoderma harzianum is respectively 3,000,000,000/g, 2,500,000,000/g.
In step (2), the fermentation condition of aspergillus oryzae are as follows: Czapek's medium, 30 DEG C of fermentation temperature, fermentation time 48 hours；
The fermentation condition of trichoderma harzianum are as follows: Czapek's medium, 35 DEG C of fermentation temperature, fermentation time 48 hours.
In step (3), by weight percentage, the composition of the culture solution is as follows: peptone 2%, sodium nitrate 1.5%,
Calcium chloride 1%, ammonium sulfate 1%, manganese sulfate 0.6%, urea 0.7%, surplus are distilled water.
In step (3), Rhizoma Atractylodis Macrocephalae slice, culture solution, seed liquor mass volume ratio be 1:20mL:10mL.
In step (3), the specific method of fermented and cultured is: pH=6, temperature 50 C, 80r/ minutes shaken cultivations 72 hours.
In step (4), in the nitration mixture aqueous solution of the lactic acid and acetic acid, the mass percent of lactic acid is 5%, the matter of acetic acid
Measuring percentage is 40%.
In step (5), the process conditions of the steam explosion technology are as follows: Rhizoma Atractylodis Macrocephalae powder is packed into the explosion of steam blasting equipment
In chamber, pressure is discharged rapidly after being kept for 2 minutes under the conditions of 1MPa.
In step (5), the process conditions of ultra micro nanometer pulverization are as follows: use airslide disintegrating mill, stream pressure 1100kPa,
Charging rate is 180r/min, and grade frequency 35Hz, grinding time is 60 minutes.
Embodiment 5:
A kind of production method of the prepared slices of Chinese crude drugs, the specific steps are as follows:
(1) after fresh Rhizoma Atractylodis Macrocephalae robs washing, slice obtains Rhizoma Atractylodis Macrocephalae slice, spare；
(2) it prepares seed liquor: respectively obtaining respective fermentation liquid after aspergillus oryzae and trichoderma harzianum are individually fermented, then
Mixing, obtains seed mixture liquid；
(3) slice of Rhizoma Atractylodis Macrocephalae obtained by step (1) is soaked in culture solution, seed mixture liquid obtained by step (2) is then added,
Fermented and cultured is taken out, the Rhizoma Atractylodis Macrocephalae that must ferment slice；
(4) fermentation Rhizoma Atractylodis Macrocephalae slice obtained by step (3) is 3 hours stifling in 85 DEG C using the nitration mixture aqueous solution of lactic acid and acetic acid,
The ethanol solution (mass concentration of stannous chloride is 8%) for immersing stannous chloride takes out rapidly afterwards, and then 400W microwave treatment 8 is divided
Clock must activate Rhizoma Atractylodis Macrocephalae slice；
(5) activation Rhizoma Atractylodis Macrocephalae slice obtained by step (4) is crushed to the Rhizoma Atractylodis Macrocephalae powder of partial size 0.8mm, then uses steam blasting
Technology is handled, then ultra micro nanometer pulverization.
Wherein, in step (1), slice thickness 3mm.
In step (2), in seed mixture liquid, the content of aspergillus oryzae and trichoderma harzianum is respectively 3,500,000,000/g, 2,000,000,000/g.
In step (2), the fermentation condition of aspergillus oryzae are as follows: Czapek's medium, 32 DEG C of fermentation temperature, fermentation time 35 hours；
The fermentation condition of trichoderma harzianum are as follows: Czapek's medium, 37 DEG C of fermentation temperature, fermentation time 32 hours.
In step (3), by weight percentage, the composition of the culture solution is as follows: peptone 2.5%, sodium nitrate
1.4%, calcium chloride 1.1%, ammonium sulfate 0.9%, manganese sulfate 0.65%, urea 0.6%, surplus is distilled water.
In step (3), Rhizoma Atractylodis Macrocephalae slice, culture solution, seed liquor mass volume ratio be 1:25mL:9mL.
In step (3), the specific method of fermented and cultured is: pH=7, temperature 45 C, 90r/ minutes shaken cultivations 65 hours.
In step (4), in the nitration mixture aqueous solution of the lactic acid and acetic acid, the mass percent of lactic acid is 8%, the matter of acetic acid
Measuring percentage is 38%.
In step (5), the process conditions of the steam explosion technology are as follows: Rhizoma Atractylodis Macrocephalae powder is packed into the explosion of steam blasting equipment
In chamber, pressure is discharged rapidly after being kept for 1 minute under the conditions of 2MPa.
In step (5), the process conditions of ultra micro nanometer pulverization are as follows: use airslide disintegrating mill, stream pressure 1100kPa,
Charging rate is 180r/min, and grade frequency 35Hz, grinding time is 70 minutes.
Comparative example 1
Step omits aspergillus oryzae in (2), remaining is the same as embodiment 1.
Comparative example 2
Step omits trichoderma harzianum in (2), remaining is the same as embodiment 1.
Comparative example 3
Aspergillus oryzae is replaced with aspergillus niger in step (2), remaining is the same as embodiment 1.
Comparative example 4
With the nitration mixture aqueous solution of lactic acid aqueous solution replacement lactic acid and acetic acid, the mass concentration of lactic acid aqueous solution in step (4)
It is 40%, remaining is the same as embodiment 1.
Comparative example 5
It is dried replacement 300W microwave treatment 5 minutes 2 hours in step (4) with 80 DEG C, remaining is the same as embodiment 1.
Comparative example 6
Step omits steam blasting step in (5), remaining is the same as embodiment 1.
Comparative example 7
Raw Rhizoma Atractylodis Macrocephalae is directly crushed, 80 meshes are crossed, is not necessarily to any processing, obtains raw Rhizoma Atractylodis Macrocephalae pulverized product.
Test example
1, active constituent content measuring is tested
Atractylenolide is the main active of Rhizoma Atractylodis Macrocephalae, using atractylenolide as the index components of Rhizoma Atractylodis Macrocephalae.Using height
Effect liquid phase chromatogram method, to the Rhizoma Atractylodis Macrocephalae for deriving from the same place of production, respectively according to Examples 1 to 5 and comparative example 1~5 and comparative example 7
The prepared slices of Chinese crude drugs are made, each 10 batches carry out the assay of atractylenolide, take atractylenolide content in 10 batches
Median records the atractylenolide content maximum value and minimum value in 10 batches as active constituent content, as a result sees
Table 1.
Chromatographic condition: chromatographic column: Kromasil-C18 column (4.6 × 250mm, 5 μm), mobile phase: methanol-water (volume ratio
80:20), flow velocity: 1.0mL/min, ultraviolet detection wavelength: 276nm, column temperature: 30 DEG C, 10 μ L of sample volume.
The preparation of solution: weighing that atractylenolide reference substance is appropriate, adds methanol dissolution that the reference substance that every 1mL contains 40 μ g is made
Solution.The life Rhizoma Atractylodis Macrocephalae pulverized product 0.5g of Examples 1 to 5, the atractylodes slice of comparative example 1~5 and comparative example 7 is weighed respectively, it is accurate
Calibration, is placed in triangular flask, adds methanol 10mL, weighed weight ultrasonic extraction 15 minutes, supplies the weight of less loss, solution
After crossing 0.45 μm of miillpore filter, test solution is obtained.
1. active constituent content of table
As shown in Table 1, compared with comparative example 7, the production method batch wise differences of Examples 1 to 5 are small, having in gained medicine materical crude slice
Effective component content is considerably higher.Comparative example 1 omits aspergillus oryzae, and comparative example 2 omits trichoderma harzianum, and comparative example 3 is replaced with aspergillus niger
Aspergillus oryzae is changed, effective component discharges insufficient, the lactic acid aqueous solution suffocating treatment of comparative example 4, and Rhizoma Atractylodis Macrocephalae effective component is vulnerable to broken
Bad, microwave treatment is replaced with conventional drying by comparative example 5, and effective component release is insufficient.
2, fluidity determining
Angle of repose measurement is carried out to Examples 1 to 5 and 5~6 gained atractylodes slice of comparative example using Repose angle gauge,
It the results are shown in Table 2.
2. fluidity determining of table
| Angle of repose (°) | |
| Embodiment 1 | 27 |
| Embodiment 2 | 27 |
| Embodiment 3 | 27 |
| Embodiment 4 | 27 |
| Embodiment 5 | 27 |
| Comparative example 5 | 33 |
| Comparative example 6 | 30 |
As can be seen from Table 2, the angle of repose of medicine materical crude slice obtained by Examples 1 to 5 is small, and mobility is more preferable.Comparative example 5 will be at microwave
Reason replaces with conventional drying, and product mobility is bad, and comparative example 6 omits steam blasting step, and product mobility is bad, explanation
Microwave treatment and steam blasting have a direct impact product mobility.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of production method of the prepared slices of Chinese crude drugs, which is characterized in that specific step is as follows:
(1) after fresh Rhizoma Atractylodis Macrocephalae robs washing, slice obtains Rhizoma Atractylodis Macrocephalae slice, spare；
(2) it prepares seed liquor: respectively obtaining respective fermentation liquid after aspergillus oryzae and trichoderma harzianum are individually fermented, then mix
It closes, obtains seed mixture liquid；
(3) slice of Rhizoma Atractylodis Macrocephalae obtained by step (1) is soaked in culture solution, seed mixture liquid obtained by step (2), fermentation is then added
Culture is taken out, the Rhizoma Atractylodis Macrocephalae that must ferment slice；
(4) fermentation Rhizoma Atractylodis Macrocephalae slice obtained by step (3) is using the nitration mixture aqueous solution of lactic acid and acetic acid in 80~90 DEG C stifling 3~4 small
When, it is taken out rapidly after immersing the ethanol solution of stannous chloride, then 300~400W microwave treatment 5~10 minutes, obtains activation Rhizoma Atractylodis Macrocephalae
Slice；
(5) activation Rhizoma Atractylodis Macrocephalae slice obtained by step (4) is crushed to the Rhizoma Atractylodis Macrocephalae powder of 0.5~1mm of partial size, then uses steam blasting skill
Art is handled, then ultra micro nanometer pulverization.
2. a kind of production method of prepared slices of Chinese crude drugs according to claim 1, which is characterized in that in step (1), slice thickness
For 2~5mm.
3. a kind of production method of prepared slices of Chinese crude drugs according to claim 1, which is characterized in that in step (2), seed mixture
In liquid, the content of aspergillus oryzae and trichoderma harzianum is respectively 30~4,000,000,000/g, 15~2,500,000,000/g.
4. a kind of production method of prepared slices of Chinese crude drugs according to claim 1, which is characterized in that in step (2), aspergillus oryzae
Fermentation condition are as follows: Czapek's medium, 30~35 DEG C of fermentation temperature, fermentation time 24~48 hours；The fermentation item of trichoderma harzianum
Part are as follows: Czapek's medium, 35~40 DEG C of fermentation temperature, fermentation time 24~48 hours.
5. a kind of production method of prepared slices of Chinese crude drugs according to claim 1, which is characterized in that in step (3), with weight hundred
Divide than meter, the composition of the culture solution is as follows: peptone 2~3%, sodium nitrate 1.2~1.5%, calcium chloride 1~1.2%, sulfuric acid
Ammonium 0.8~1%, manganese sulfate 0.6~0.7%, urea 0.5~0.7%, surplus are distilled water.
6. a kind of production method of prepared slices of Chinese crude drugs according to claim 1, which is characterized in that in step (3), Rhizoma Atractylodis Macrocephalae is cut
Piece, culture solution, seed liquor mass volume ratio be 1:20~30mL:8~10mL.
7. a kind of production method of prepared slices of Chinese crude drugs according to claim 1, which is characterized in that in step (3), fermented and cultured
Specific method be: pH=6~7,40~50 DEG C of temperature, 80~100r/ minutes shaken cultivations 58~72 hours.
8. a kind of production method of prepared slices of Chinese crude drugs according to claim 1, which is characterized in that in step (4), the lactic acid
In the nitration mixture aqueous solution of acetic acid, the mass percent of lactic acid is 5~10%, and the mass percent of acetic acid is 35~40%.
9. a kind of production method of prepared slices of Chinese crude drugs according to claim 1, which is characterized in that in step (5), the steam
The process conditions of blasting technique are as follows: Rhizoma Atractylodis Macrocephalae powder is fitted into the explosion chamber of steam blasting equipment, is kept under the conditions of 1~2.5MPa
Pressure is discharged rapidly after 1~2 minute.
10. a kind of production method of prepared slices of Chinese crude drugs according to claim 1, which is characterized in that in step (5), ultra micro is received
The broken process conditions of rice flour are as follows: use airslide disintegrating mill, stream pressure 1100kPa, charging rate 180r/min, classification frequency
Rate is 35Hz, and grinding time is 60~80 minutes.
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| CN105596397A (en) * | 2016-01-05 | 2016-05-25 | 北京农学院 | Fermented rhizoma atractylodis macrocephalae and preparation method and application thereof |
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