CN109549861B - Oligopeptide-containing skin repair freeze-dried powder - Google Patents
Oligopeptide-containing skin repair freeze-dried powder Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0216—Solid or semisolid forms
- A61K8/022—Powders; Compacted Powders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/732—Starch; Amylose; Amylopectin; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/007—Preparations for dry skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/81—Preparation or application process involves irradiation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
Abstract
The invention relates to the technical field of cosmetics, in particular to oligopeptide-containing skin repair freeze-dried powder, which comprises the following raw materials in parts by weight: 75-85 parts of deionized water, 2-8 parts of D-mannitol, 0.3-0.7 part of trehalose, 0.2-0.6 part of pullulan, 0.0007-0.0014 part of oligopeptide, 4-9 parts of Erycibe obtusifolia extract and 5-12 parts of radix scrophulariae extract; the skin repair freeze-dried powder can quickly permeate into skin, not only can quickly repair various skin injuries including skin problems of skin aging, wrinkles, color spots, dryness, sensitivity, red blood streak and the like, but also can continuously enhance the immunity and the resistance of the skin; can promote proliferation of epithelial cells and fibroblasts; enhancing the viability of epidermal cells; delaying the aging of epidermal cells and rapidly restoring all the components of the skin to the optimal physiological state.
Description
Technical Field
The invention relates to the technical field of cosmetics, and in particular relates to oligopeptide-containing skin repair freeze-dried powder.
Background
The skin needs to be repaired every day, and natural wind, frost, snow, rain, ultraviolet light, serious environmental pollution, food additives, functional components in skin care products, fruit acid hormones, lead and mercury, antibiotics, pigments and the like can damage the self resistance and immunity of the skin, cause damage to the health of the skin to different degrees and cause the problems of premature aging, wrinkles, color spots, acne, dryness, sensitivity, red blood streak and the like of the skin. Therefore, timely repair is needed when skin damage occurs, and the repair is beneficial and harmless for preventing and treating various skin problems.
The habit of people is that the skin care product can be used frequently to have a good care effect on the skin; in fact, this is not necessarily so! Firstly, in order to pursue effects, attract customers and increase sales, a plurality of skin care product manufacturers add some functional components, such as acid, hormone, lead and mercury, antibiotics and the like, into the product, which harms the skin and causes a plurality of skin beautifying sequelae; secondly, the skin care product without the functional components cannot be denied, and many essential components in the skin care product are very beneficial to the skin, but the skin care product must be added with preservatives, and the strong bacteriostatic action of the preservatives can cause serious damage to the ecological environment of the skin. The real skin repair is to comprehensively improve the immunity of the skin, so that the skin is recovered and kept in a relatively healthy state.
The true repair product should be safe and effective, be used for a long period of time, and be a product of any brand of care and efficacy, capable of preventing and timely repairing possible skin damage. Therefore, the ecological restoration skin care product without adding any preservative should be selected judiciously, and is a great invention in the field of skin care nowadays.
At present, in the research of cosmetics, freeze-dried powder cosmetics appear, when the freeze-dried powder cosmetics are used, the freeze-dried powder cosmetics need to be matched with water liquid for dissolving freeze-dried powder, and when the existing water liquid for dissolving freeze-dried powder is matched with the freeze-dried powder cosmetics, the skin injury can be repaired. However, the traditional freeze-dried powder has poor repairing effect on skin, and the duration time and the duration effect of the repairing effect are not ideal.
Disclosure of Invention
The invention aims to solve the technical problem of providing the oligopeptide-containing skin repair freeze-dried powder, and the oligopeptide, the Erycibe obtusifolia extract and the radix scrophulariae extract are added into the skin repair freeze-dried powder, so that the skin repair freeze-dried powder can quickly permeate into the skin, quickly repair various skin injuries and quickly restore all components of the skin to the optimal physiological state.
The technical problem to be solved by the invention is realized by the following technical scheme:
the oligopeptide-containing skin repair freeze-dried powder comprises the following raw materials in parts by weight: 75-85 parts of deionized water, 2-8 parts of D-mannitol, 0.3-0.7 part of trehalose, 0.2-0.6 part of pullulan, 0.0007-0.0014 part of oligopeptide, 4-9 parts of Erycibe obtusifolia extract and 5-12 parts of radix scrophulariae extract.
As a preferred scheme, the skin repair freeze-dried powder comprises the following raw materials in parts by weight: 78-83 parts of deionized water, 4-6 parts of D-mannitol, 0.4-0.6 part of trehalose, 0.45-0.55 part of pullulan, 0.0008-0.0012 part of oligopeptide, 5-7 parts of Erycibe obtusifolia extract and 6-9 parts of radix scrophulariae extract.
As an optimal scheme, the skin repair freeze-dried powder comprises the following raw materials in parts by weight: 79.999 parts of deionized water, 5 parts of D-mannitol, 0.5 part of trehalose, 0.5 part of pullulan, 0.001 part of oligopeptide, 6 parts of Erycibe obtusifolia extract and 8 parts of radix scrophulariae extract.
Preferably, the oligopeptide is oligopeptide-3.
As a preferred embodiment, the erycibe obtusifolia extract is prepared by a method comprising the following steps:
(1) slicing the erycibe obtusifolia, crushing, sieving by a 80-mesh sieve, and infiltrating the erycibe obtusifolia powder with ethanol at normal temperature for 20-50 minutes;
(2) preheating ethanol with the mass fraction of 70-85% to 80-90 ℃, adding the ethanol into the mixture obtained in the step (1), reducing the pressure to 0.2-0.3 MPa, and extracting for 2-3.5 hours;
(3) centrifuging the mixture obtained in the step (2), repeating the step (2) on filter residues, repeating the extraction for 2 times, combining filtrates, standing, and filtering;
(4) and (4) dealcoholizing, concentrating and drying the clarified liquid obtained in the step (3) to obtain the Erycibe obtusifolia extract.
As a preferred embodiment, the figwort root extract is prepared by a method comprising the following steps:
(1) taking radix scrophulariae as a raw material, crushing, and screening with a 30-40-mesh screen;
(2) transferring the material obtained in the step (1) into a microwave extraction device, adding 75-85% of ethanol which is 5-7 times of the mass of the raw material, heating the material to 45-50 ℃, extracting for 15-20 min under the microwave power of 3-4 kilowatts, and collecting the extracting solution obtained by microwave treatment;
(3) filtering the extracting solution obtained in the step (2) by using a 400-500-mesh filter screen to obtain an extracting solution with solid impurities removed;
(4) and (4) concentrating the extracting solution obtained in the step (3) at the temperature of 40-55 ℃ and the vacuum degree of 0.05-0.1 MPa, and drying to obtain the radix scrophulariae extract.
As a preferred scheme, the freeze-dried powder for skin repair is prepared by a method comprising the following steps:
(1) putting D-mannitol, trehalose and pullulan into a container, adding deionized water, and stirring to obtain a mixture 1;
(2) filtering the mixture 1 through 500-mesh filter cloth, sterilizing the filtrate at high temperature, standing until the temperature of the solution is reduced to normal temperature, sequentially adding the oligopeptide, the Erycibe obtusifolia extract and the radix scrophulariae extract, fully mixing uniformly, subpackaging in an aseptic container, performing low-temperature freeze drying, and finally taking out after plugging under vacuum condition to obtain the oligopeptide-containing skin-repairing freeze-dried powder.
As a preferable scheme, the stirring conditions in the step (1) are as follows: the rotating speed is 1500-2000 r/min, and the time is 40-60 minutes.
As a preferable scheme, the high-temperature sterilization temperature in the step (2) is 120-130 ℃, and the sterilization time is 30-60 minutes.
Preferably, the freeze-drying time in the step (2) is 25 to 35 hours.
Has the advantages that: the oligopeptide-containing skin repair freeze-dried powder disclosed by the invention can be rapidly permeated into skin by adding the oligopeptides, the Erycibe obtusifolia extract and the radix scrophulariae extract and adding scientific and reasonable proportioning of other raw materials and by adopting a freeze-drying technology and reasonably controlling preparation parameters, so that various skin injuries including skin aging, wrinkles, color spots, dryness, sensitivity, redness and the like can be rapidly repaired, and the immunity and the resistance of the skin can be continuously enhanced; the components in the raw material formula supplement each other, and the prepared skin repair freeze-dried powder has a synergistic effect on the effects of promoting the proliferation of epithelial cells and fibroblasts, enhancing the activity of epidermal cells, delaying the aging of epidermal cells and quickly restoring all the components of skin to the optimal physiological state, so that the synergistic effect on quickly repairing skin injury is particularly obvious.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are a part of the embodiments of the present invention, but not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1 skin repair lyophilized powder containing oligopeptide
The raw material formula is as follows: 79.999 parts of deionized water, 5 parts of D-mannitol, 0.5 part of trehalose, 0.5 part of pullulan, 30.001 parts of oligopeptide, 6 parts of Erycibe obtusifolia extract and 8 parts of radix scrophulariae extract;
the erycibe obtusifolia extract is prepared by the following steps:
(1) slicing caulis Erycibes, pulverizing, sieving with 80 mesh sieve, and soaking caulis Erycibes powder with ethanol at room temperature for 40 min;
(2) heating 80% ethanol to 88 deg.C, adding into the mixture obtained in step (1), and extracting under reduced pressure to 0.25MPa for 3 hr;
(3) centrifuging the mixture obtained in the step (2), repeating the step (2) on filter residues, repeating the extraction for 2 times, combining filtrates, standing, and filtering;
(4) dealcoholizing, concentrating and drying the clarified liquid obtained in the step (3) to obtain a erycibe obtusifolia extract;
the figwort root extract is prepared by the following steps:
(1) taking radix scrophulariae as a raw material, crushing, and screening with a 30-mesh screen;
(2) transferring the material obtained in the step (1) into a microwave extraction device, adding 80% ethanol with the mass fraction of 6 times that of the raw material, heating the material to 48 ℃, extracting for 18 min under the microwave power of 3.5 kilowatts, and collecting the extracting solution obtained by microwave treatment;
(3) filtering the extracting solution obtained in the step (2) by using a 500-mesh filter screen to obtain the extracting solution with solid impurities removed;
(4) and (4) concentrating the extracting solution obtained in the step (3) at the temperature of 50 ℃ and the vacuum degree of 0.08 MPa, and drying to obtain the radix scrophulariae extract.
The preparation method of the oligopeptide-containing skin repair freeze-dried powder comprises the following steps:
(1) placing D-mannitol, trehalose and pullulan into a container, adding deionized water, and stirring for 50 minutes at 1800 r/min to obtain a mixture 1;
(2) filtering the mixture 1 through 500-mesh filter cloth, sterilizing the filtrate at the high temperature of 125 ℃ for 45 minutes, standing, cooling the solution to the normal temperature, sequentially adding the oligopeptide, the Erycibe obtusifolia extract and the radix scrophulariae extract, fully and uniformly mixing, subpackaging in an aseptic container, performing low-temperature freeze drying for 30 hours, and finally taking out after tamponade under the vacuum condition to obtain the oligopeptide-containing skin-repairing freeze-dried powder.
Example 2 skin repair lyophilized powder containing oligopeptide
The raw material formula is as follows: 75 parts of deionized water, 2 parts of D-mannitol, 0.3 part of trehalose, 0.2 part of pullulan, 30.0007 parts of oligopeptide, 4 parts of Erycibe obtusifolia extract and 5 parts of radix scrophulariae extract;
the preparation method of the Erycibes obtusifolia extract is the same as that of the example 1;
the preparation method of the figwort root extract is the same as that of the embodiment 1;
the preparation method of the oligopeptide-containing skin repair freeze-dried powder is the same as that of the example 1.
Example 3 skin repair lyophilized powder containing oligopeptide
The raw material formula is as follows: 85 parts of deionized water, 8 parts of D-mannitol, 0.7 part of trehalose, 0.6 part of pullulan, 30.0014 parts of oligopeptide, 9 parts of Erycibe obtusifolia extract and 12 parts of radix scrophulariae extract;
the preparation method of the Erycibes obtusifolia extract is the same as that of the example 1;
the preparation method of the figwort root extract is the same as that of the embodiment 1;
the preparation method of the oligopeptide-containing skin repair freeze-dried powder is the same as that of the example 1.
Example 4 an oligopeptide-containing lyophilized powder for skin repair
The raw material formula is the same as that in the example 1, except that the preparation operation parameters are different in the process of preparing the freeze-dried powder; the preparation method of the Erycibes obtusifolia extract is the same as that of the example 1;
the preparation method of the figwort root extract is the same as that of the embodiment 1;
the preparation method of the oligopeptide-containing skin repair freeze-dried powder comprises the following steps:
(1) placing D-mannitol, trehalose and pullulan into a container, adding deionized water, and stirring at 1500 r/min for 40 minutes to obtain a mixture 1;
(2) filtering the mixture 1 through 500-mesh filter cloth, sterilizing the filtrate at the high temperature of 120 ℃ for 30 minutes, standing, cooling the solution to the normal temperature, sequentially adding the oligopeptide, the Erycibe obtusifolia extract and the radix scrophulariae extract, fully and uniformly mixing, subpackaging in an aseptic container, carrying out low-temperature freeze drying for 25 hours, and finally taking out after tamponade under the vacuum condition to obtain the oligopeptide-containing skin-repairing freeze-dried powder.
Example 5 an oligopeptide-containing lyophilized powder for skin repair
The raw material formula is the same as that in the example 1, except that the preparation operation parameters are different in the process of preparing the freeze-dried powder; the preparation method of the Erycibes obtusifolia extract is the same as that of the example 1;
the preparation method of the figwort root extract is the same as that of the embodiment 1;
the preparation method of the oligopeptide-containing skin repair freeze-dried powder comprises the following steps:
(1) placing D-mannitol, trehalose and pullulan into a container, adding deionized water, and stirring at 2000 r/min for 60 minutes to obtain a mixture 1;
(2) filtering the mixture 1 through 500-mesh filter cloth, sterilizing the filtrate at a high temperature of 130 ℃ for 60 minutes, standing, cooling the solution to normal temperature, sequentially adding the oligopeptide, the Erycibe obtusifolia extract and the radix scrophulariae extract, fully and uniformly mixing, subpackaging in an aseptic container, performing low-temperature freeze drying for 35 hours, and finally taking out after tamponade under a vacuum condition to obtain the oligopeptide-containing skin-repairing freeze-dried powder.
Comparative example 1
Comparative example 1 is different from example 1 in that 0.0005 parts by weight of Erycibes extract and 0.0005 parts by weight of Scrophularia ningpoensis extract are added in addition, oligopeptide-3 is not added, and the remaining components and contents are the same as those of example 1; the preparation method of the Erycibes obtusifolia extract is the same as that of the example 1; the preparation method of the figwort root extract is the same as that of the embodiment 1; the preparation method of the oligopeptide-containing skin repair freeze-dried powder is the same as that of the example 1.
Comparative example 2
Comparative example 2 is different from example 1 in that 3 weight parts of oligopeptide-3 and 3 weight parts of radix scrophulariae extract are added, no Erycibe obtusifolia extract is added, and the rest components and contents are the same as example 1; the preparation method of the figwort root extract is the same as that of the embodiment 1; the preparation method of the oligopeptide-containing skin repair freeze-dried powder is the same as that of the example 1.
Comparative example 3
Comparative example 3 is different from example 1 in that 4 parts by weight of oligopeptide-3 and 4 parts by weight of Erycibe obtusifolia extract are added, the radix scrophulariae extract is not added, and the rest components and contents are the same as those of example 1; the preparation method of the Erycibes obtusifolia extract is the same as that of the example 1; the preparation method of the oligopeptide-containing skin repair freeze-dried powder is the same as that of the example 1.
Comparative example 4
Comparative example 4 is different from example 1 in that 14 parts by weight of oligopeptide-3 was added, Erycibe obtusifolia extract and Scrophularia ningpoensis extract were not added, and the remaining components and contents were the same as example 1; the preparation method of the oligopeptide-containing skin repair freeze-dried powder is the same as that of the example 1.
Comparative example 5
Comparative example 5 is different from example 1 in that 8.001 parts by weight of more Erycibes extract was added, no oligopeptide-3 and figwort extract were added, and the remaining components and contents were the same as example 1; the preparation method of the Erycibes obtusifolia extract is the same as that of the example 1; the preparation method of the oligopeptide-containing skin repair freeze-dried powder is the same as that of the example 1.
Comparative example 6
Comparative example 6 is different from example 1 in that 6.001 parts by weight of radix scrophulariae extract was added, oligopeptide-3 and Erycibe obtusifolia extract were not added, and the rest components and contents were the same as example 1; the preparation method of the figwort root extract is the same as that of the embodiment 1; the preparation method of the oligopeptide-containing skin repair freeze-dried powder is the same as that of the example 1.
Comparative example 7
Comparative example 7 is different from example 1 in that oligopeptide-3 in the raw material formulation is replaced by oligopeptide-1, and the rest components and contents are the same as example 1; the preparation method of the Erycibes obtusifolia extract is the same as that of the example 1; the preparation method of the figwort root extract is the same as that of the embodiment 1; the preparation method of the oligopeptide-containing skin repair freeze-dried powder is the same as that of the example 1.
Comparative example 8
The difference between the comparative example 8 and the example 1 is that oligopeptide-6 is used to replace oligopeptide-3 in the raw material formula, and the rest components and content are the same as those in the example 1; the preparation method of the Erycibes obtusifolia extract is the same as that of the example 1; the preparation method of the figwort root extract is the same as that of the embodiment 1; the preparation method of the oligopeptide-containing skin repair freeze-dried powder is the same as that of the example 1.
Comparative example 9
Comparative example 9 is different from example 1 in that a commercially available Erycibes nigrum extract and a commercially available Scrophularia ningpoensis extract are used instead of the Erycibes nigrum extract and the Scrophularia ningpoensis extract obtained by the preparation method of the present invention, and the remaining components and contents are the same as those of example 1; the preparation method of the oligopeptide-containing skin repair freeze-dried powder is the same as that of the example 1.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Test example: test for promoting skin repair Effect
1. Test materials: the skin repair freeze-dried powder prepared in the examples 1 to 5 and the comparative examples 1 to 9.
2. Test subjects: 150 female Wistar rats weighing 250g were selected.
3. The test method comprises the following steps: 150 rats were randomly divided into 15 groups of 10 rats each, example 1, example 2, example 3, example 4, example 5, comparative 1 and comparative 2, comparative 3, comparative 4, comparative 5, comparative 6, comparative 7, comparative 8, comparative 9.
Two sides of the spinal column of the back of the rat are laterally lcm, the head and the tail of the rat are parallel to the spinal column and are respectively marked with a radius of 1cm and an area of 3.14cm2The circular incision line of (1). After the skin is disinfected by iodophor, the whole skin is cut off to the deep fascia along the marked line by scissors, and a circular wound surface is formed. The skin repair freeze-dried powders prepared in examples 1-5 and comparative examples 1-9 are used on the left side respectively, wounds on the right side are not treated and are used as a control group, the control group is wrapped with gauze in a covering mode, and continuous observation is carried out for 9 d.
4. And (3) test results:
in 9d, the average wound area of the mice was observed relative to the wound area on the first day, and the specific results are shown in table 1.
TABLE 1 mean wound area size at different times
As can be seen from the data in table 1, the wound area is the smallest, the improvement rate of skin repair is the best, and the continuous repair effect of skin is the best in the same time relative to the first day by using the embodiment 1 as the best technical scheme; the skin repairing lyophilized powder obtained in the embodiments 1 and 2-3 has a slightly poorer skin repairing effect than that of the embodiment 1 after use if the content ratio of the raw materials is different from that of the embodiment 1; from the embodiment 1 and the embodiments 4 to 5, if the operation parameters in the process of preparing the freeze-dried powder are different from those in the embodiment 1, the skin continuous repairing effect of the obtained skin repairing freeze-dried powder after use is slightly worse than that of the embodiment 1; as can be seen from example 1 and comparative examples 1 to 3, if the raw material formula only contains any two of oligopeptide-3, erycibe obtusifolia extract and figwort root extract, the skin-repairing freeze-dried powder obtained after use has a skin-repairing effect inferior to that of example 1, wherein the skin-repairing effect is inferior when the raw material formula only contains erycibe obtusifolia extract and figwort root extract but does not contain oligopeptide-3, namely comparative example 1; as can be seen from the example 1 and the comparative examples 4 to 6, if the raw material formula only contains any one of oligopeptide-3, Erycibe obtusifolia extract and radix scrophulariae extract, the skin-repairing effect of the obtained skin-repairing freeze-dried powder after use is far inferior to that of the example 1; from the example 1 and the comparative examples 7 to 8, it is known that if oligopeptide-1 or oligopeptide-6 is used to replace oligopeptide-3 in the raw material formula, the skin repairing effect of the skin repairing lyophilized powder of the example 1 cannot be achieved; as can be seen from example 1 and comparative example 9, if the commercially available Erycibes extract and the commercially available Scrophularia ningpoensis extract are used to replace the Erycibes extract and the Scrophularia ningpoensis extract obtained by the preparation method of the present invention, the skin care effect of the obtained lyophilized powder is far inferior to that of example 1.
Claims (4)
1. The oligopeptide-containing skin repair freeze-dried powder is characterized by comprising the following raw materials in parts by weight: 79.999 parts of deionized water, 5 parts of D-mannitol, 0.5 part of trehalose, 0.5 part of pullulan, 0.001 part of oligopeptide, 6 parts of Erycibe obtusifolia extract and 8 parts of radix scrophulariae extract;
the oligopeptide is oligopeptide-3;
the erycibe obtusifolia extract is prepared by a method comprising the following steps:
(1) slicing the erycibe obtusifolia, crushing, sieving by a 80-mesh sieve, and infiltrating the erycibe obtusifolia powder with ethanol at normal temperature for 20-50 minutes;
(2) preheating ethanol with the mass fraction of 70-85% to 80-90 ℃, adding the ethanol into the mixture obtained in the step (1), reducing the pressure to 0.2-0.3 MPa, and extracting for 2-3.5 hours;
(3) centrifuging the mixture obtained in the step (2), repeating the step (2) on filter residues, repeating the extraction for 2 times, combining filtrates, standing, and filtering;
(4) dealcoholizing, concentrating and drying the clarified liquid obtained in the step (3) to obtain a erycibe obtusifolia extract;
the figwort root extract is prepared by a method comprising the following steps:
(1) taking radix scrophulariae as a raw material, crushing, and screening with a 30-40-mesh screen;
(2) transferring the material obtained in the step (1) into a microwave extraction device, adding 75-85% of ethanol which is 5-7 times of the mass of the raw material, heating the material to 45-50 ℃, extracting for 15-20 min under the microwave power of 3-4 kilowatts, and collecting the extracting solution obtained by microwave treatment;
(3) filtering the extracting solution obtained in the step (2) by using a 400-500-mesh filter screen to obtain an extracting solution with solid impurities removed;
(4) concentrating the extracting solution obtained in the step (3) at the temperature of 40-55 ℃ and the vacuum degree of 0.05-0.1 MPa, and drying to obtain a radix scrophulariae extract;
the oligopeptide-containing skin repair freeze-dried powder is prepared by a method comprising the following steps:
(1) putting D-mannitol, trehalose and pullulan into a container, adding deionized water, and stirring to obtain a mixture 1;
(2) filtering the mixture 1 through 500-mesh filter cloth, sterilizing the filtrate at high temperature, standing until the temperature of the solution is reduced to normal temperature, sequentially adding the oligopeptide, the Erycibe obtusifolia extract and the radix scrophulariae extract, fully mixing uniformly, subpackaging in an aseptic container, performing low-temperature freeze drying, and finally taking out after plugging under vacuum condition to obtain the oligopeptide-containing skin-repairing freeze-dried powder.
2. The oligopeptide-containing skin-repair lyophilized powder according to claim 1, wherein the stirring conditions in step (1) in the preparation method of the skin-repair lyophilized powder are as follows: the rotating speed is 1500-2000 r/min, and the time is 40-60 minutes.
3. The oligopeptide-containing freeze-dried skin repair powder according to claim 1, wherein the temperature for high-temperature sterilization in the step (2) in the preparation method of the freeze-dried skin repair powder is 120-130 ℃, and the sterilization time is 30-60 minutes.
4. The oligopeptide-containing freeze-dried powder for skin repair according to claim 1, wherein the freeze-drying time in the step (2) in the preparation method of the freeze-dried powder for skin repair is 25-35 hours.
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| CN111518754A (en) * | 2020-05-06 | 2020-08-11 | 潍坊峡山源宜医学科技有限责任公司 | Preparation method and application of intracellular active ingredients of human skin fibroblasts |
| CN114983836A (en) * | 2022-06-15 | 2022-09-02 | 广州陶妆生物科技有限公司 | Repairing type freeze-dried wadding and preparation method thereof |
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