CN109549615A - The device and method of oxygen saturation endoscope monitoring optical dynamic therapy effect - Google Patents
The device and method of oxygen saturation endoscope monitoring optical dynamic therapy effect Download PDFInfo
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- CN109549615A CN109549615A CN201811439213.9A CN201811439213A CN109549615A CN 109549615 A CN109549615 A CN 109549615A CN 201811439213 A CN201811439213 A CN 201811439213A CN 109549615 A CN109549615 A CN 109549615A
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- 229910052760 oxygen Inorganic materials 0.000 title claims abstract description 132
- 239000001301 oxygen Substances 0.000 title claims abstract description 132
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 title claims abstract description 130
- 230000003287 optical effect Effects 0.000 title claims abstract description 86
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- 230000009471 action Effects 0.000 claims abstract description 5
- 230000008557 oxygen metabolism Effects 0.000 claims abstract description 5
- 230000002792 vascular Effects 0.000 claims abstract description 5
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/04—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor combined with photographic or television appliances
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0073—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by tomography, i.e. reconstruction of 3D images from 2D projections
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- A—HUMAN NECESSITIES
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- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0082—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes
- A61B5/0084—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for introduction into the body, e.g. by catheters
- A61B5/0086—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for introduction into the body, e.g. by catheters using infrared radiation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue
- A61B5/1455—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue using optical sensors, e.g. spectral photometrical oximeters
- A61B5/14551—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue using optical sensors, e.g. spectral photometrical oximeters for measuring blood gases
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue
- A61B5/1455—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue using optical sensors, e.g. spectral photometrical oximeters
- A61B5/1459—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue using optical sensors, e.g. spectral photometrical oximeters invasive, e.g. introduced into the body by a catheter
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- A61B5/00—Measuring for diagnostic purposes; Identification of persons
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- A61B5/4836—Diagnosis combined with treatment in closed-loop systems or methods
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- A61B5/48—Other medical applications
- A61B5/4848—Monitoring or testing the effects of treatment, e.g. of medication
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N5/00—Radiation therapy
- A61N5/06—Radiation therapy using light
- A61N5/0613—Apparatus adapted for a specific treatment
- A61N5/062—Photodynamic therapy, i.e. excitation of an agent
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N5/00—Radiation therapy
- A61N5/06—Radiation therapy using light
- A61N5/067—Radiation therapy using light using laser light
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Abstract
The present invention relates to a kind of device and method of oxygen saturation endoscope monitoring optical dynamic therapy effect, comprising: in advance after injection photosensitizer to destination organization；Destination organization is irradiated using the light source that endoscope imports, function or metamorphosis occur for induction destination organization, and then lead to cellular damage and necrosis effect, as optical dynamic therapy, the participation that this effect must be aerobic.Therefore, the oxygen distribution image by comparison destination organization before and after photodynamic action quantitatively calculates the variation of each point oxygen saturation value to reflect the variation of oxygen metabolism in fine vascular in therapeutic process, to monitor optical dynamic therapy effect in real time.
Description
Technical field
The present invention relates to medicine and medical instrument.The specifically device of oxygen saturation endoscope monitoring optical dynamic therapy effect
And method.
Background technique
Optical dynamic therapy (PDT) acts on the selectivity intake and retention of photosensitizer using sick cell, is allowed to certain
It is more in time to retain in pathological tissues, at this time with the laser irradiation lesions position of specific wavelength, divide in biological tissues
Under the participation of sub- oxygen, strong photochemical reaction can be induced, to destroy blood vessel and inhibit new vessels, to reach treatment mesh
's.PDT has the function of that tissue selectivity is good, toxic side effect is low, treatment time is short, can protect the features such as appearance and vitals,
Recently as its reaching its maturity technically, it is widely used to tumour, skin disease, inflammation, ophthalmology disease and infection class
Disease etc..In recent years, near infrared spectrum (NIRS) method realizes noninvasive, the continuous monitoring of blood oxygen saturation in tissue.When
Angiocarpy beating it is faint even with extracorporal circulatory system when, pulse oximetry non-metering, but tissue oxygenation non-destructive monitoring is still
Information can be provided.Therefore, near infrared light blood oxygen detection method has been used to monitoring brain, muscle, flap, the local organizations such as tumour
Blood transports situation.With NIRS measurement oxygen saturation rather than blood oxygen saturation can greatly reduce cost；Oxygen content is important in PDT
Parameter, therefore, with NIRS detection PDT therapeutic effect be an ideal selection.And oxygen is measured with endoscope made of this
Saturation degree is sensitive, quick come the device performance for monitoring optical dynamic therapy effect, at low cost, noninvasive, quantitative, continuous, excellent in real time
Point.
Summary of the invention
It is an object of the invention in view of the deficienciess of the prior art, to provide a kind of oxygen saturation endoscope monitoring light dynamic
The device and method of power therapeutic effect.The characteristics of being distributed using the oxygen content of oxygen saturation endoscopic technique measurement destination organization,
By the damage effect that the variation of oxygen content distribution and oxygen saturation value before and after optical dynamic therapy is assessed to blood vessel.It is operated
Performance sensitive, quick, expense is low, can accurately monitor optical dynamic therapy effect in real time.
The present invention is monitored for destination organization in its optical dynamic therapy effect of research；Moreover, the present invention is not limited only to this, also
It can be used for the organism of other monitoring oxygen contents.
Another object of the present invention is to provide a kind of endoscope apparatus for realizing the above method.
The method of oxygen saturation endoscope of the present invention monitoring optical dynamic therapy effect include: had accumulated in laser irradiation it is photosensitive
During the destination organization of agent carries out optical dynamic therapy, oxygen distribution image of the destination organization before and after photodynamic action is compared,
Reflect the change of destination organization by the quantitative change for calculating oxygen saturation numerical value, to monitor the effect of optical dynamic therapy in real time
Fruit.
Further, in advance after injection photosensitizer to destination organization；Endoscope import laser irradiation destination organization into
Row optical dynamic therapy compares oxygen distribution image of the destination organization before and after photodynamic action, passes through quantitative calculating each point oxygen saturation
The variation of angle value is to reflect the changes in oxygen metabolism of capilary in therapeutic process, to monitor target optical dynamic therapy effect in real time.
Further, it is placed in target tissue surface with interior snooping head, destination organization is carried out with dual wavelength near-infrared light source
Tomoscan closes the principle with reduced hemoglobin in the difference measurement oxygen saturation of near infrared absorbing coefficient using oxygen, and use is black
White high sensitivity Near Infrared CCD video camera shoots the blood distribution figure of available destination organization.
Further, comprising the following steps:
Step s100: before optical dynamic therapy, by the destination organization of endoscope position tissue, before scanning obtains optical dynamic therapy
The oxygen distribution image of destination organization and corresponding quantitative oxygen saturation value；
Step s200 carries out optical dynamic therapy: i.e. injection photosensitizer, with corresponding photosensitizer after photosensitizer concentrates on destination organization
The light source of absorbing wavelength irradiates, and makes destination organization that thermal response and irreversible chemical reaction occur；
Step s300, in optical dynamic therapy irradiation process, continuous scanning obtains the oxygen distribution of destination organization when optical dynamic therapy
Image and corresponding quantitative oxygen saturation value；
Step s400 continues to scan on to obtain the oxygen distribution image of destination organization after optical dynamic therapy after optical dynamic therapy
And corresponding quantitative oxygen saturation value；
Step s500, oxygen distribution image and corresponding quantitative oxygen saturation value to the destination organization of acquisition carry out data processing,
The effect of optical dynamic therapy is assessed by the comparison of oxygen metabolism in pretherapy and post-treatment capilary.
Further, in the step s100 and step s400 light source assembly use oxygen saturation endoscope light source；Institute
Step s200 light source is stated using photodynamic therapy light source；The step s300 uses two kinds of light sources simultaneously.
The photodynamic therapy light source and double-wavelength light source are confocal；Photodynamic therapy light source depends on the absorption of photosensitizer
Peak；Double-wavelength light source is respectively 760nm and 850nm；If the absorption peak of photosensitizer is between 760nm and 850nm, double
Wavelength light source may act as photodynamic therapy light source.Two kinds of light sources are LED light fibre output light source；What light source front end was placed synchronizes list
There are two circular hole on wavelength filter switch, double-wavelength light source is emitted from different circular holes, scans positioned destination organization；
Light source is connected to probe by optical fiber；Optical power density is up to 0.1 ~ 1W/cm when irradiation at short distance focal area2Magnitude
Further, the scanning process in the step s100, step s300 and step s400 is the control system by computer
System software control synchronous control system；The light source of synchronous control system control light source transmitting is taken the photograph with black and white near-infrared high sensitivity CCD
Camera is synchronous, when the light source of light source transmitting passes through a certain optical filter, via the trigger signal that synchronous control system is fed back, computer
Control System Software be connected to after the trigger signal of synchronous control system to black and white near-infrared high sensitivity CCD camera shooting instruction
And complete automatically scanning process.
Further, the collection process in the step s100, step s300 and step s400 be by data collecting card without
The optical imagery of distortion ground acquisition near-infrared high sensitivity CCD camera photographic subjects tumor tissues, and vision signal is converted into
Digital signal is simultaneously transmitted to computer；Image processing software algorithm realization in computer highlights tiny blood vessels and focal zone
Domain.
Further, the quantitative scoring of oxygen content distribution map and oxygen content passes through in computer at last in the step s500
Image processing software independently derives the calculating of oxygen content according to improved Lambertian-Beer law and absorbance adduction law
Formula, oxygen imaging diagnosis system show the vascular distribution figure of tissue and quantitatively provide local oxygen content.
Further, a kind of device for realizing the above method, including light source assembly, Single wavelength optical-filter switcher, optics
Coupler, interior snooping head assembly, near-infrared high sensitivity CCD camera, signal acquisition component, computer and synchronous control system；
The signal acquisition component, control assembly and computer are successively electrically connected；The Single wavelength optical-filter switcher is arranged in institute
State the front end of light source assembly；There are two circular holes on the Single wavelength optical-filter switcher, are respectively the window that 760nm wavelength passes through
The window that mouth and 850nm wavelength pass through；In this way, Single wavelength optical-filter switcher only passes through a kind of wavelength within the single time, it is real
Existing Single wavelength imaging；
The interior snooping head assembly includes interior snooping head and probe-protecting device, before probe-protecting device is installed on interior snooping head
End；The interior snooping head includes that the optical fiber of a branch of transmission light source and multi beam collect the optical fiber for scattering light；The outermost of interior snooping head
It is the conduit to shield；The output end of the optical fiber of the transmission light source is spherical expansion tip；
The optical coupler is installed between Single wavelength optical-filter switcher and interior snooping head assembly；Light source assembly issues double wave
Long near infrared light is emitted from Single wavelength optical-filter switcher, and optically coupled device enters the optical fiber of transmission light source, then through transmitting
The scattering light of the output end Scan orientation destination organization of the optical fiber of light source, destination organization excitation is collected the optical fiber of scattering light by multi beam
It collects；
Synchronous control system in light source assembly light source and near-infrared high sensitivity CCD camera be connected with conducting wire or data line；
Meanwhile synchronous control system is connected with computer.
The optical fiber that the multi beam collects scattering light is connected by optical coupler with near-infrared high sensitivity CCD camera；
The near-infrared high sensitivity CCD camera is electrically connected with data collecting card, and the data collecting card is connect with computer；
Control System Software and image processing software are installed in the computer；Described image processing software includes that oxygen imaging is examined
Disconnected system；The oxygen imaging diagnosis system includes digital image acquisition system, oxygen imaging algorithm system, oxygen value assistant analysis system
System.
Further, the light source in the light source assembly includes the light of photodynamic therapy light source and oxygen saturation endoscope
Source；The light source of the oxygen saturation endoscope is dual wavelength near infrared light, and dual wavelength near infrared light includes 760nm near infrared light
With 850nm near infrared light；The light source of optical dynamic therapy in light source assembly depends on the absorbing wavelength of photosensitizer, when it is positioned at double
When the region of wavelength light source 760nm and 850nm, the light source of photodynamic therapy light source and oxygen saturation endoscope is used in conjunction with same
Light source；Light source in the light source assembly is LED light source.
Further, the synchronous control system is made of single-chip microcontroller and relay, and single-chip microcontroller controls relay, relay
Duration and the transfer sequence of light source assembly transmitting light source are controlled, while single-chip microcontroller controls probe assembly, control near-infrared is high
The scattering light that sensitivity CCD camera energy sync pulse jamming is excited to Single wavelength each time.
The present invention compared with prior art have following advantages and effects
(1) high-resolution of the present invention.
(2) present invention monitors the numerical value change of tissue oxygenation saturation using oxygen saturation technology, with traditional venous oxygen
Saturation degree monitoring, is a kind of undamaged monitoring technology, is capable of providing and monitors in body.
(3) the method for the present invention can directly utilize the absolute value of oxygen saturation quantitative expression oxygen content, so can accomplish
The degree of impairment of the accurate optical dynamic therapy process medium vessels of monitoring in real time.
(4) in the present invention light source selected be both optical dynamic therapy simultaneously and the light source of oxygen content monitoring, it is possible to it is real
Integration is now treated and monitored, provides a kind of more convenient and fast method for clinical research.
(5) for the device of the invention using the oxygen calculation formula simplified, the cost of each component is lower, especially uses LED light
Source combination, so the cost of single unit system is also relatively low, is easy to application.
Detailed description of the invention
Fig. 1 is the structural schematic diagram that oxygen saturation endoscope monitors optical dynamic therapy effects device.
Fig. 2 is the interior snooping head cross-sectional view that oxygen saturation endoscope monitors optical dynamic therapy effect.
Fig. 3 oxygen saturation endoscope of the present invention monitors optical dynamic therapy effect imaging device work step figure.
Fig. 4 is the flow chart that oxygen saturation endoscope monitors optical dynamic therapy effect method.
In figure: 1, light source assembly；2, Single wavelength optical-filter switcher；3, the window that 760nm wavelength passes through；4,850nm wave
The long window passed through；5, the optical fiber of transmission light source；6, the optical fiber of scattering light is collected；7, probe-protecting device；8, conduit；9, synchronous
Control system；10, near-infrared high sensitivity CCD camera；11, signal acquisition component；12, computer；13, optical coupler.
Specific embodiment
The device of optical dynamic therapy effect is monitored to this oxygen saturation endoscope below with reference to specific example and attached drawing
It is described in detail with the technical solution of method, so that it is clearer.
It please refers to shown in Fig. 1, the structure of oxygen saturation endoscope monitoring optical dynamic therapy effect imaging device of the present invention is shown
It is intended to;Described device includes light source assembly 1, Single wavelength optical-filter switcher 2, optical coupler 13, interior snooping head assembly, close red
Outer high sensitivity CCD camera 10, signal acquisition component 11, computer 12 and synchronous control system 9；The signal acquisition component,
Control assembly is successively electrically connected with computer.
Light source in the light source assembly 1 includes the light source of photodynamic therapy light source and oxygen saturation endoscope；The oxygen
The light source of saturation degree endoscope is dual wavelength near infrared light, and dual wavelength near infrared light includes 760nm near infrared light and 850nm close
Infrared light；Light source in the light source assembly 1 is LED light source；
The front end of the light source assembly 1, the Single wavelength optical-filter switcher 2 is arranged in the Single wavelength optical-filter switcher 2
Upper there are two circular holes, are respectively the window 3 that 760nm wavelength passes through and the window 4 that 850nm wavelength passes through.
The interior snooping head assembly includes interior snooping head and probe-protecting device 7；Probe-protecting device 7 is installed on interior snooping
The front end of head plays the role of spying upon head in protection；It please refers to shown in Fig. 2, the interior snooping head includes a branch of transmission light source
Optical fiber 5 and multi beam collect the optical fiber 6 of scattering light；The outermost of interior snooping head is the conduit 8 to shield；The transmission light source
Optical fiber 5 output end be spherical expansion tip.
The optical coupler 13 is installed between Single wavelength optical-filter switcher 2 and interior snooping head assembly；Light source assembly 1
Dual wavelength near infrared light is issued, dual wavelength near infrared light is emitted from two circular holes of Single wavelength optical-filter switcher 2, through optics coupling
Clutch 13 enters the optical fiber 5 of transmission light source, then the output end Scan orientation destination organization of the optical fiber 5 through transmission light source, target
The scattering light of excitation is organized to be collected by the optical fiber 6 that multi beam collects scattering light.
The synchronous control system 9 is made of single-chip microcontroller and relay, and single-chip microcontroller controls relay, and relay controls light source
Component 1 emits duration and the transfer sequence of light source, while single-chip microcontroller controls probe assembly, controls near-infrared high sensitivity CCD
The scattering light that 10 energy sync pulse jamming of video camera is excited to Single wavelength each time；Light source in synchronous control system 9 and light source assembly 1
It is connected with near-infrared high sensitivity CCD camera 10 with conducting wire or data line.Meanwhile synchronous control system 9 is connected with computer 12.
The optical fiber 6 that the multi beam collects scattering light passes through optical coupler 13 and near-infrared high sensitivity CCD camera 10
It is connected；The near-infrared high sensitivity CCD camera 10 is electrically connected with data collecting card 11, the data collecting card 11 and calculating
Machine 12 connects；Data collecting card 11 acquires the telecommunications for each wavelength near infrared light that near-infrared high sensitivity CCD camera 10 is shot
Number；Meanwhile the electric signal that near-infrared high sensitivity CCD camera 10 is shot is converted to digital signal and is sent to computer 12.
Control System Software and image processing software are installed in the computer 12.Described image processing software includes oxygen
Imaging diagnosis system.Computer 12 controls synchronous control system 9 by Control System Software；Computer 10 is acquired by data
The data image of each wavelength near infrared light needed for card 11 obtains oxygen imaging algorithm；Then, the number of each wavelength near infrared light
According to image input oxygen imaging diagnosis system obtain each point containing oxygen value.
The oxygen imaging diagnosis system includes digital image acquisition system, oxygen imaging algorithm system, oxygen value assistant analysis system
System.The optical imagery of each wavelength near infrared light needed for oxygen imaging algorithm can be obtained by digital image acquisition system.Number
Image synthesis and image procossing are carried out according to image input oxygen imaging algorithm system, Pseudo Col ored Image is carried out to image later, it will be black
The gray value of white image is mapped to corresponding colour.Oxygen value diagnostic system for doctor provide content and oxygen analysis function and to
Quantitative result out.
Wherein, the light source of the optical dynamic therapy in light source assembly 1 depends on the absorbing wavelength of photosensitizer, when it is located at double wave
When the region of long light source 760nm and 850nm, the light source of photodynamic therapy light source and oxygen saturation endoscope is used in conjunction with same light
Source, i.e. selection dual wavelength near-infrared LED.
The near-infrared high sensitivity CCD camera 10 is black and white high sensitivity Near Infrared CCD video camera 10, is used to image group
Knit the optical imagery of inner surface；Near-infrared high sensitivity CCD camera 10 uses high-definition camera.
The Single wavelength optical-filter switcher 2 is used to ensure in the single time only realize that unicast grows up to by a kind of wavelength
Picture.
The data collecting card 11 is black and white high-definition image capture card, can without distortions be taken the photograph near-infrared high sensitivity CCD
The video signal collection that camera 10 inputs guarantees the accuracy of raw image data to computer 12.
It please refers to shown in Fig. 3, when oxygen saturation endoscope monitoring optical dynamic therapy effect imaging device of the present invention works:
(1) initialization step realizes that two kinds of light sources are confocal and the synchronous operation of light source and camera.The unicast being sequentially connected is provided
Long length filter switch 2, near-infrared high sensitivity CCD camera 10, data collecting card 11, oxygen imaging diagnosis system；
(2) destination organization for passing through endoscope position tissue, is shone with the light source of photodynamic therapy light source and oxygen saturation endoscope
It penetrates；
(3) scanning step controls synchronous control system 9 by the Control System Software of computer.Synchronous control system 9 controls light
The transmitting light source of source component 1 is synchronous with black and white near-infrared high sensitivity CCD camera 10, when a certain optical filter has light transmission, via
The trigger signal that synchronous control system 9 is fed back, the Control System Software of computer 12 are connected to the trigger signal of synchronous control system 9
Automatic collection process is completed to 10 shooting instruction of black and white near-infrared high sensitivity CCD camera afterwards.Therefore, one acquisition process can
To obtain the image data of several different Single wavelengths；
(4) video acquisition step, it is swollen that data collecting card 11 acquires 10 photographic subjects of near-infrared high sensitivity CCD camera without distortions
The optical imagery of tumor tissue, and vision signal is converted into digital signal and is transmitted to computer 12；Image in computer 12
The realization of processing software algorithm highlights tiny blood vessels and focal area；
(5) the quantitative calculating of oxygen content distribution map and oxygen content.Image processing software in computer 12 is according to improved lambert
Spy-Beer law and absorbance adduction law independently derive the calculation formula of oxygen content, oxygen imaging diagnosis system display group
The vascular distribution figure knitted and quantitatively provide local oxygen content.
Above-mentioned oxygen saturation endoscope monitors photodynamic therapy effect method, passes through the target of endoscope position tissue
Tissue, near-infrared high sensitivity CCD camera 10 acquire the organism optical image, provide video image, data collecting card 11 is nearly
The vision signal that infrared high sensitivity CCD camera 10 inputs is converted into digital signal and is transmitted to computer 12, oxygen imaging diagnosis
System is realized to full depth accurately image is organized, and the accuracy of detection is improved.
Before optical dynamic therapy, repeat step (1)-(5), obtain before optical dynamic therapy the oxygen distribution image of destination organization and
Corresponding quantitative oxygen saturation value；
During optical dynamic therapy, injection photosensitizer repeats step (1)-after photosensitizer concentrates on destination organization first
(5), obtain destination organization in optical dynamic therapy oxygen distribution image and corresponding quantitative oxygen saturation value；
After optical dynamic therapy, repeat step (1)-(5), obtain after optical dynamic therapy the oxygen distribution image of destination organization and
Corresponding quantitative oxygen saturation value.
Fig. 4 oxygen saturation endoscope monitoring optical dynamic therapy effect method is please referred to, specifically includes the following steps:
Step s100: before optical dynamic therapy, scanning obtains before optical dynamic therapy the oxygen distribution image of destination organization and corresponding
Quantitative oxygen saturation value；
Step s200 carries out optical dynamic therapy: i.e. injection photosensitizer, with corresponding photosensitizer after photosensitizer concentrates on destination organization
The light source of absorbing wavelength irradiates, and makes destination organization that thermal response and irreversible chemical reaction occur；
Step s300, in optical dynamic therapy irradiation process, continuous scanning obtains the oxygen distribution of destination organization when optical dynamic therapy
Image and corresponding quantitative oxygen saturation value；
Step s400 continues to scan on to obtain the oxygen distribution image of destination organization after optical dynamic therapy after optical dynamic therapy
And corresponding quantitative oxygen saturation value；
Step s500, oxygen distribution image and corresponding quantitative oxygen saturation value to the destination organization of acquisition carry out data processing,
The effect of optical dynamic therapy is assessed by the comparison of oxygen metabolism in pretherapy and post-treatment capilary.
In described step s100, s300 and s300, photodynamic therapy light source is confocal with double-wavelength light source；Optical dynamic therapy light
Source depends on the absorption peak of photosensitizer；Double-wavelength light source is respectively 760nm and 850nm；If the absorption peak of photosensitizer between
Between 760nm and 850nm, then double-wavelength light source may act as photodynamic therapy light source.Two kinds of light sources are LED light fibre output light
Source.There are two circular hole on the synchronization Single wavelength optical-filter switcher that light source front end is placed, double-wavelength light source goes out from different circular holes
It penetrates, scans positioned destination organization；Light source is connected to probe by optical fiber；Optical power density when irradiation at short distance focal area
Up to 0.1 ~ 1W/cm2Magnitude.
In the step s100, control system is made of devices such as single-chip microcontroller and relays, and single-chip microcontroller controls relay, after
Electrical equipment control light source duration and transfer sequence, while single-chip microcontroller control light source is synchronous with camera CCD.
In described step s100, s300 and s400, scanning step: under the action of control circuit, dual wavelength passes through unicast
The successive irradiating sample of long length filter switch, while camera receives the sample signal of 760nm wavelength and the sample of 850nm wavelength respectively
Product signal.
In the step s200, the absorbing wavelength of the photosensitizer is photodynamic therapy light source, and two kinds of technologies use simultaneously
Diagnosing and treating integration may be implemented.
In described step s100, s300 and s400, the output end of output optical fibre described in component is peeped in the probe as spherical shape
Extend tip.
In described step s100, s300 and s400, light source and CCD camera are run simultaneously in probe assembly, by single-chip microcontroller control
System.
In described step s100, s300 and s400, acquisition system acquires the tissue light of inner surface by the CCD camera
It learns image and is converted into digital signal, the digital data transmission is saved and shown with imaging unit to the control.
In described step s100, s300 and s400, data collecting card is connected to the rear end CCD, and acquisition digital signal simultaneously adopt by handle
Image processing system of the digital data transmission collected to computer；
In described step s100, s300 and s400, the signal processing circuit of system include sync separator, adjust amplifier group,
Synchronous integrator, sampling hold circuit, the circuit modules such as lock-in amplifier.
In the step s500, oxygen imaging algorithm system, oxygen value quantify computing system, thus obtain sample oxygen Butut with
And local oxygen content value.
In the step s500, software calibration: the determination of system tentatively calibrated need to be adjusted by analyzing experimental data
Section, the result of adjusting make the blood oxygen level of healthy individuals 76% or so.System further calibrates needs and a variety of method phases
In conjunction with more person-times of measurement results compare, and obtain more accurate check formula and coefficient by regression analysis.
The device and method of the oxygen saturation endoscope monitoring optical dynamic therapy effect of invention described above can not have to
Vascular contrast agent is the structure of display blood vessel, therefore to the mutual identification between blood vessel, lump, lymph node and blood vessel structure, there is it
Distinctive feature.Its high resolution sensitively detects the metabolic alterations of morphological element's medium sized vein oxygen, can monitor optical dynamic therapy in time
Effect.
One embodiment of the present invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. the method for oxygen saturation endoscope monitoring optical dynamic therapy effect, which is characterized in that injection photosensitizer to target in advance
After tissue；Optical dynamic therapy is carried out in the laser irradiation destination organization that endoscope imports, compares destination organization in photodynamic action
The oxygen distribution image of front and back reflects the oxo of capilary in therapeutic process by the quantitative variation for calculating each point oxygen saturation value
Thank to variation, to monitor target optical dynamic therapy effect in real time.
2. the method for oxygen saturation endoscope monitoring optical dynamic therapy effect, which is characterized in that be placed in target group with interior snooping head
Surface is knitted, tomoscan is carried out to destination organization with dual wavelength near-infrared light source, is closed using oxygen and reduced hemoglobin is close red
The principle of the difference measurement oxygen saturation of outer absorptivity, shoots available mesh with black and white high sensitivity Near Infrared CCD video camera
Mark the blood distribution figure of tissue.
3. method according to claim 1 or 2, which comprises the following steps:
Step s100: before optical dynamic therapy, by the destination organization of endoscope position tissue, before scanning obtains optical dynamic therapy
The oxygen distribution image of destination organization and corresponding quantitative oxygen saturation value；
Step s200 carries out optical dynamic therapy: i.e. injection photosensitizer, with corresponding photosensitizer after photosensitizer concentrates on destination organization
The light source of absorbing wavelength irradiates, and makes destination organization that thermal response and irreversible chemical reaction occur；
Step s300, in optical dynamic therapy irradiation process, continuous scanning obtains the oxygen distribution of destination organization when optical dynamic therapy
Image and corresponding quantitative oxygen saturation value；
Step s400 continues to scan on to obtain the oxygen distribution image of destination organization after optical dynamic therapy after optical dynamic therapy
And corresponding quantitative oxygen saturation value；
Step s500, oxygen distribution image and corresponding quantitative oxygen saturation value to the destination organization of acquisition carry out data processing,
The effect of optical dynamic therapy is assessed by the comparison of oxygen metabolism in pretherapy and post-treatment capilary.
4. according to the method described in claim 3, it is characterized in that, light source assembly uses in the step s100 and step s400
The light source of oxygen saturation endoscope；The step s200 light source uses photodynamic therapy light source；The step s300 is used simultaneously
Two kinds of light sources.
5. according to the method described in claim 3, it is characterized in that, in the step s100, step s300 and step s400
Scanning process is to control synchronous control system by the Control System Software of computer；Synchronous control system controls light source transmitting
Light source is synchronous with black and white near-infrared high sensitivity CCD camera, when the light source of light source transmitting passes through a certain optical filter, controls via synchronous
The trigger signal of system feedback processed, the Control System Software of computer are connected to after the trigger signal of synchronous control system close to black and white
Infrared high sensitivity CCD camera shooting instruction and complete automatically scanning process.
6. according to the method described in claim 3, it is characterized in that, in the step s100, step s300 and step s400
Collection process is the optics for acquiring near-infrared high sensitivity CCD camera photographic subjects tumor tissues without distortions by data collecting card
Image, and vision signal is converted into digital signal and is transmitted to computer；Image processing software algorithm reality in computer
Now highlight tiny blood vessels and focal area.
7. according to the method described in claim 3, it is characterized in that, oxygen content distribution map and oxygen content in the step s500
Quantitative scoring passes through the image processing software in computer at last and sums it up law according to improved Lambertian-Beer law and absorbance
Independently derive the calculation formula of oxygen content, oxygen imaging diagnosis system shows the vascular distribution figure of tissue and quantitatively provides part
Oxygen content.
8. method according to claim 1 or 2, a kind of device for realizing claim 1-3 the method, feature exist
In, including light source assembly, Single wavelength optical-filter switcher, optical coupler, interior snooping head assembly, near-infrared high sensitivity CCD take the photograph
Camera, signal acquisition component, computer and synchronous control system；The signal acquisition component, control assembly and computer are successively
Electrical connection；The front end of the light source assembly is arranged in the Single wavelength optical-filter switcher；The unicast long length filter switching
There are two circular holes on device, are respectively the window that 760nm wavelength passes through and the window that 850nm wavelength passes through；In this way, Single wavelength is filtered
Mating plate switch, only by a kind of wavelength, realizes Single wavelength imaging within the single time；
The interior snooping head assembly includes interior snooping head and probe-protecting device, before probe-protecting device is installed on interior snooping head
End；The interior snooping head includes that the optical fiber of a branch of transmission light source and multi beam collect the optical fiber for scattering light；The outermost of interior snooping head
It is the conduit to shield；The output end of the optical fiber of the transmission light source is spherical expansion tip；
The optical coupler is installed between Single wavelength optical-filter switcher and interior snooping head assembly；Light source assembly issues double wave
Long near infrared light is emitted from Single wavelength optical-filter switcher, and optically coupled device enters the optical fiber of transmission light source, then through transmitting
The scattering light of the output end Scan orientation destination organization of the optical fiber of light source, destination organization excitation is collected the optical fiber of scattering light by multi beam
It collects；
Synchronous control system in light source assembly light source and near-infrared high sensitivity video camera be connected with conducting wire or data line；Together
When, synchronous control system is connected with computer；
The optical fiber that the multi beam collects scattering light is connected by optical coupler with near-infrared high sensitivity CCD camera；It is described
Near-infrared high sensitivity CCD camera is electrically connected with data collecting card, and the data collecting card is connect with computer；
Control System Software and image processing software are installed in the computer；Described image processing software includes that oxygen imaging is examined
Disconnected system；The oxygen imaging diagnosis system includes digital image acquisition system, oxygen imaging algorithm system, oxygen value assistant analysis system
System.
9. device according to claim 8, which is characterized in that the light source in the light source assembly includes optical dynamic therapy light
The light source in source and oxygen saturation endoscope；The light source of the oxygen saturation endoscope is dual wavelength near infrared light, and dual wavelength is closely red
Outer light includes 760nm near infrared light and 850nm near infrared light；The light source of optical dynamic therapy in light source assembly depends on photosensitive
The absorbing wavelength of agent, when it is located at the region of double-wavelength light source 760nm and 850nm, photodynamic therapy light source and oxygen saturation
The light source of endoscope is used in conjunction with same light source；Light source in the light source assembly is LED light source.
10. device according to claim 8, which is characterized in that the synchronous control system is by single-chip microcontroller and relay group
At single-chip microcontroller controls relay, and relay controls duration and the transfer sequence of light source assembly transmitting light source, while single-chip microcontroller
Control probe assembly, the scattering light that control near-infrared high sensitivity CCD camera energy sync pulse jamming is excited to Single wavelength each time.
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