CN109548950A - The polypeptide and its preparation process in a kind of aurelia source and application - Google Patents
The polypeptide and its preparation process in a kind of aurelia source and application Download PDFInfo
- Publication number
- CN109548950A CN109548950A CN201811560639.XA CN201811560639A CN109548950A CN 109548950 A CN109548950 A CN 109548950A CN 201811560639 A CN201811560639 A CN 201811560639A CN 109548950 A CN109548950 A CN 109548950A
- Authority
- CN
- China
- Prior art keywords
- aurelia
- polypeptide
- magnesium
- calcium
- zinc
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000242587 Aurelia Species 0.000 title claims abstract description 85
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 61
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 59
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 58
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 239000000203 mixture Substances 0.000 claims abstract description 37
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 34
- 239000002738 chelating agent Substances 0.000 claims abstract description 25
- 239000008367 deionised water Substances 0.000 claims abstract description 24
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 24
- 230000002779 inactivation Effects 0.000 claims abstract description 17
- 230000001376 precipitating effect Effects 0.000 claims abstract description 14
- 102000004190 Enzymes Human genes 0.000 claims abstract description 13
- 108090000790 Enzymes Proteins 0.000 claims abstract description 13
- 108091005804 Peptidases Proteins 0.000 claims abstract description 13
- 239000004365 Protease Substances 0.000 claims abstract description 13
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 13
- 229910052751 metal Inorganic materials 0.000 claims abstract description 12
- 239000002184 metal Substances 0.000 claims abstract description 12
- 150000003839 salts Chemical class 0.000 claims abstract description 12
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 11
- 238000006243 chemical reaction Methods 0.000 claims abstract description 9
- 238000006911 enzymatic reaction Methods 0.000 claims abstract description 9
- 239000000706 filtrate Substances 0.000 claims abstract description 9
- 238000001914 filtration Methods 0.000 claims abstract description 9
- 238000003756 stirring Methods 0.000 claims abstract description 9
- 239000002245 particle Substances 0.000 claims abstract description 8
- 239000013535 sea water Substances 0.000 claims abstract description 8
- 238000001035 drying Methods 0.000 claims abstract description 7
- 238000007710 freezing Methods 0.000 claims abstract description 6
- 230000008014 freezing Effects 0.000 claims abstract description 6
- 239000000047 product Substances 0.000 claims abstract description 5
- 239000002537 cosmetic Substances 0.000 claims abstract description 4
- 239000003814 drug Substances 0.000 claims abstract description 4
- 230000036541 health Effects 0.000 claims abstract description 4
- 239000000654 additive Substances 0.000 claims description 18
- 230000000996 additive effect Effects 0.000 claims description 18
- 241000242583 Scyphozoa Species 0.000 claims description 14
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N Iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 claims description 12
- 229940088598 enzyme Drugs 0.000 claims description 12
- 235000019419 proteases Nutrition 0.000 claims description 12
- RGHNJXZEOKUKBD-SQOUGZDYSA-N D-gluconic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 claims description 10
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 10
- 239000011777 magnesium Substances 0.000 claims description 10
- 229910052749 magnesium Inorganic materials 0.000 claims description 10
- 229940091250 magnesium supplement Drugs 0.000 claims description 10
- ODHCTXKNWHHXJC-UHFFFAOYSA-N 5-oxoproline Chemical compound OC(=O)C1CCC(=O)N1 ODHCTXKNWHHXJC-UHFFFAOYSA-N 0.000 claims description 9
- AFSDNFLWKVMVRB-UHFFFAOYSA-N Ellagic acid Chemical compound OC1=C(O)C(OC2=O)=C3C4=C2C=C(O)C(O)=C4OC(=O)C3=C1 AFSDNFLWKVMVRB-UHFFFAOYSA-N 0.000 claims description 9
- ATJXMQHAMYVHRX-CPCISQLKSA-N Ellagic acid Natural products OC1=C(O)[C@H]2OC(=O)c3cc(O)c(O)c4OC(=O)C(=C1)[C@H]2c34 ATJXMQHAMYVHRX-CPCISQLKSA-N 0.000 claims description 9
- 229920002079 Ellagic acid Polymers 0.000 claims description 9
- 229960005069 calcium Drugs 0.000 claims description 9
- 239000011575 calcium Substances 0.000 claims description 9
- 229910052791 calcium Inorganic materials 0.000 claims description 9
- 229960002852 ellagic acid Drugs 0.000 claims description 9
- 235000004132 ellagic acid Nutrition 0.000 claims description 9
- FAARLWTXUUQFSN-UHFFFAOYSA-N methylellagic acid Natural products O1C(=O)C2=CC(O)=C(O)C3=C2C2=C1C(OC)=C(O)C=C2C(=O)O3 FAARLWTXUUQFSN-UHFFFAOYSA-N 0.000 claims description 9
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 8
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 8
- ZLNQQNXFFQJAID-UHFFFAOYSA-L magnesium carbonate Chemical compound [Mg+2].[O-]C([O-])=O ZLNQQNXFFQJAID-UHFFFAOYSA-L 0.000 claims description 8
- 239000001095 magnesium carbonate Substances 0.000 claims description 8
- 229910000021 magnesium carbonate Inorganic materials 0.000 claims description 8
- -1 calcium carboxylates Chemical class 0.000 claims description 7
- TUSDEZXZIZRFGC-UHFFFAOYSA-N 1-O-galloyl-3,6-(R)-HHDP-beta-D-glucose Natural products OC1C(O2)COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC1C(O)C2OC(=O)C1=CC(O)=C(O)C(O)=C1 TUSDEZXZIZRFGC-UHFFFAOYSA-N 0.000 claims description 6
- 239000001263 FEMA 3042 Substances 0.000 claims description 6
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 6
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 6
- CPLXHLVBOLITMK-UHFFFAOYSA-N Magnesium oxide Chemical compound [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 claims description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Penta-digallate-beta-D-glucose Natural products OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 claims description 6
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 6
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 claims description 6
- 239000003513 alkali Substances 0.000 claims description 6
- LRBQNJMCXXYXIU-QWKBTXIPSA-N gallotannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@H]2[C@@H]([C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-QWKBTXIPSA-N 0.000 claims description 6
- YIXJRHPUWRPCBB-UHFFFAOYSA-N magnesium nitrate Chemical compound [Mg+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O YIXJRHPUWRPCBB-UHFFFAOYSA-N 0.000 claims description 6
- NUJOXMJBOLGQSY-UHFFFAOYSA-N manganese dioxide Chemical compound O=[Mn]=O NUJOXMJBOLGQSY-UHFFFAOYSA-N 0.000 claims description 6
- 235000015523 tannic acid Nutrition 0.000 claims description 6
- 229920002258 tannic acid Polymers 0.000 claims description 6
- 229940033123 tannic acid Drugs 0.000 claims description 6
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 claims description 6
- 108091005508 Acid proteases Proteins 0.000 claims description 5
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 claims description 5
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims description 5
- 239000000174 gluconic acid Substances 0.000 claims description 5
- 235000012208 gluconic acid Nutrition 0.000 claims description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 5
- 108010038851 tannase Proteins 0.000 claims description 5
- 229910052725 zinc Inorganic materials 0.000 claims description 5
- 239000011701 zinc Substances 0.000 claims description 5
- 108010011619 6-Phytase Proteins 0.000 claims description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 4
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 claims description 4
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 claims description 4
- FMRLDPWIRHBCCC-UHFFFAOYSA-L Zinc carbonate Chemical compound [Zn+2].[O-]C([O-])=O FMRLDPWIRHBCCC-UHFFFAOYSA-L 0.000 claims description 4
- VSGNNIFQASZAOI-UHFFFAOYSA-L calcium acetate Chemical compound [Ca+2].CC([O-])=O.CC([O-])=O VSGNNIFQASZAOI-UHFFFAOYSA-L 0.000 claims description 4
- 239000001639 calcium acetate Substances 0.000 claims description 4
- 235000011092 calcium acetate Nutrition 0.000 claims description 4
- 229960005147 calcium acetate Drugs 0.000 claims description 4
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 4
- 239000001110 calcium chloride Substances 0.000 claims description 4
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 4
- 235000011148 calcium chloride Nutrition 0.000 claims description 4
- 229940085127 phytase Drugs 0.000 claims description 4
- 239000000467 phytic acid Substances 0.000 claims description 4
- 229940068041 phytic acid Drugs 0.000 claims description 4
- 235000002949 phytic acid Nutrition 0.000 claims description 4
- 239000011667 zinc carbonate Substances 0.000 claims description 4
- 235000004416 zinc carbonate Nutrition 0.000 claims description 4
- 229910000010 zinc carbonate Inorganic materials 0.000 claims description 4
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 4
- 229960001763 zinc sulfate Drugs 0.000 claims description 4
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 4
- MWWMZNITXBJVSP-DKWTVANSSA-L (2s)-2-aminobutanedioate;manganese(2+) Chemical compound [Mn+2].[O-]C(=O)[C@@H](N)CC([O-])=O MWWMZNITXBJVSP-DKWTVANSSA-L 0.000 claims description 3
- GUPMWVRHEBLNMF-DKWTVANSSA-N (2s)-2-aminobutanedioic acid;copper Chemical compound [Cu].OC(=O)[C@@H](N)CC(O)=O GUPMWVRHEBLNMF-DKWTVANSSA-N 0.000 claims description 3
- LQZFQLVBZRVDLJ-DKWTVANSSA-N (2s)-2-aminobutanedioic acid;zinc Chemical compound [Zn].OC(=O)[C@@H](N)CC(O)=O LQZFQLVBZRVDLJ-DKWTVANSSA-N 0.000 claims description 3
- MSYNCHLYGJCFFY-UHFFFAOYSA-B 2-hydroxypropane-1,2,3-tricarboxylate;titanium(4+) Chemical compound [Ti+4].[Ti+4].[Ti+4].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O MSYNCHLYGJCFFY-UHFFFAOYSA-B 0.000 claims description 3
- DLSJEZJLUPVERH-RGMNGODLSA-N C(C)(=O)N[C@@H](CCSC)C(=O)O.[Zn] Chemical compound C(C)(=O)N[C@@H](CCSC)C(=O)O.[Zn] DLSJEZJLUPVERH-RGMNGODLSA-N 0.000 claims description 3
- OPSXJNAGCGVGOG-DKWTVANSSA-L Calcium L-aspartate Chemical compound [Ca+2].[O-]C(=O)[C@@H](N)CC([O-])=O OPSXJNAGCGVGOG-DKWTVANSSA-L 0.000 claims description 3
- BCZXFFBUYPCTSJ-UHFFFAOYSA-L Calcium propionate Chemical compound [Ca+2].CCC([O-])=O.CCC([O-])=O BCZXFFBUYPCTSJ-UHFFFAOYSA-L 0.000 claims description 3
- OCUCCJIRFHNWBP-IYEMJOQQSA-L Copper gluconate Chemical compound [Cu+2].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O OCUCCJIRFHNWBP-IYEMJOQQSA-L 0.000 claims description 3
- RGHNJXZEOKUKBD-SQOUGZDYSA-M D-gluconate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O RGHNJXZEOKUKBD-SQOUGZDYSA-M 0.000 claims description 3
- 239000004471 Glycine Substances 0.000 claims description 3
- MQHWFIOJQSCFNM-UHFFFAOYSA-L Magnesium salicylate Chemical compound [Mg+2].OC1=CC=CC=C1C([O-])=O.OC1=CC=CC=C1C([O-])=O MQHWFIOJQSCFNM-UHFFFAOYSA-L 0.000 claims description 3
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 3
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 3
- WHMDKBIGKVEYHS-IYEMJOQQSA-L Zinc gluconate Chemical compound [Zn+2].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O WHMDKBIGKVEYHS-IYEMJOQQSA-L 0.000 claims description 3
- CANRESZKMUPMAE-UHFFFAOYSA-L Zinc lactate Chemical compound [Zn+2].CC(O)C([O-])=O.CC(O)C([O-])=O CANRESZKMUPMAE-UHFFFAOYSA-L 0.000 claims description 3
- QOZNOMXIWCYWHI-RGMNGODLSA-N [Cu].C(C)(=O)N[C@@H](CCSC)C(=O)O Chemical compound [Cu].C(C)(=O)N[C@@H](CCSC)C(=O)O QOZNOMXIWCYWHI-RGMNGODLSA-N 0.000 claims description 3
- ZOIORXHNWRGPMV-UHFFFAOYSA-N acetic acid;zinc Chemical compound [Zn].CC(O)=O.CC(O)=O ZOIORXHNWRGPMV-UHFFFAOYSA-N 0.000 claims description 3
- FAPWYRCQGJNNSJ-UBKPKTQASA-L calcium D-pantothenic acid Chemical compound [Ca+2].OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O.OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O FAPWYRCQGJNNSJ-UBKPKTQASA-L 0.000 claims description 3
- 229940034055 calcium aspartate Drugs 0.000 claims description 3
- FNAQSUUGMSOBHW-UHFFFAOYSA-H calcium citrate Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O FNAQSUUGMSOBHW-UHFFFAOYSA-H 0.000 claims description 3
- 239000001354 calcium citrate Substances 0.000 claims description 3
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims description 3
- 239000004227 calcium gluconate Substances 0.000 claims description 3
- 235000013927 calcium gluconate Nutrition 0.000 claims description 3
- 229960004494 calcium gluconate Drugs 0.000 claims description 3
- 239000000920 calcium hydroxide Substances 0.000 claims description 3
- 229910001861 calcium hydroxide Inorganic materials 0.000 claims description 3
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 claims description 3
- 239000001527 calcium lactate Substances 0.000 claims description 3
- 235000011086 calcium lactate Nutrition 0.000 claims description 3
- 229960002401 calcium lactate Drugs 0.000 claims description 3
- BRPQOXSCLDDYGP-UHFFFAOYSA-N calcium oxide Chemical compound [O-2].[Ca+2] BRPQOXSCLDDYGP-UHFFFAOYSA-N 0.000 claims description 3
- 239000000292 calcium oxide Substances 0.000 claims description 3
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 3
- 229960002079 calcium pantothenate Drugs 0.000 claims description 3
- NEEHYRZPVYRGPP-UHFFFAOYSA-L calcium;2,3,4,5,6-pentahydroxyhexanoate Chemical compound [Ca+2].OCC(O)C(O)C(O)C(O)C([O-])=O.OCC(O)C(O)C(O)C(O)C([O-])=O NEEHYRZPVYRGPP-UHFFFAOYSA-L 0.000 claims description 3
- 239000010941 cobalt Substances 0.000 claims description 3
- 229910017052 cobalt Inorganic materials 0.000 claims description 3
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims description 3
- 229940108925 copper gluconate Drugs 0.000 claims description 3
- ORTQZVOHEJQUHG-UHFFFAOYSA-L copper(II) chloride Chemical compound Cl[Cu]Cl ORTQZVOHEJQUHG-UHFFFAOYSA-L 0.000 claims description 3
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 3
- OPQARKPSCNTWTJ-UHFFFAOYSA-L copper(ii) acetate Chemical compound [Cu+2].CC([O-])=O.CC([O-])=O OPQARKPSCNTWTJ-UHFFFAOYSA-L 0.000 claims description 3
- 239000004222 ferrous gluconate Substances 0.000 claims description 3
- 235000013924 ferrous gluconate Nutrition 0.000 claims description 3
- 229960001645 ferrous gluconate Drugs 0.000 claims description 3
- 229940050410 gluconate Drugs 0.000 claims description 3
- 229910052742 iron Inorganic materials 0.000 claims description 3
- VRIVJOXICYMTAG-IYEMJOQQSA-L iron(ii) gluconate Chemical compound [Fe+2].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O VRIVJOXICYMTAG-IYEMJOQQSA-L 0.000 claims description 3
- UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical compound [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 claims description 3
- 239000011654 magnesium acetate Substances 0.000 claims description 3
- 235000011285 magnesium acetate Nutrition 0.000 claims description 3
- 229940069446 magnesium acetate Drugs 0.000 claims description 3
- 229940074358 magnesium ascorbate Drugs 0.000 claims description 3
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 3
- 235000011147 magnesium chloride Nutrition 0.000 claims description 3
- 235000002538 magnesium citrate Nutrition 0.000 claims description 3
- 239000004337 magnesium citrate Substances 0.000 claims description 3
- 229960005336 magnesium citrate Drugs 0.000 claims description 3
- VTHJTEIRLNZDEV-UHFFFAOYSA-L magnesium dihydroxide Chemical compound [OH-].[OH-].[Mg+2] VTHJTEIRLNZDEV-UHFFFAOYSA-L 0.000 claims description 3
- 239000000347 magnesium hydroxide Substances 0.000 claims description 3
- 229910001862 magnesium hydroxide Inorganic materials 0.000 claims description 3
- OVGXLJDWSLQDRT-UHFFFAOYSA-L magnesium lactate Chemical compound [Mg+2].CC(O)C([O-])=O.CC(O)C([O-])=O OVGXLJDWSLQDRT-UHFFFAOYSA-L 0.000 claims description 3
- 239000000626 magnesium lactate Substances 0.000 claims description 3
- 235000015229 magnesium lactate Nutrition 0.000 claims description 3
- 229960004658 magnesium lactate Drugs 0.000 claims description 3
- 239000000395 magnesium oxide Substances 0.000 claims description 3
- 229940072082 magnesium salicylate Drugs 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 229960003390 magnesium sulfate Drugs 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- AIOKQVJVNPDJKA-ZZMNMWMASA-L magnesium;(2r)-2-[(1s)-1,2-dihydroxyethyl]-4-hydroxy-5-oxo-2h-furan-3-olate Chemical compound [Mg+2].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] AIOKQVJVNPDJKA-ZZMNMWMASA-L 0.000 claims description 3
- NFFJLMKHRCXLJO-UHFFFAOYSA-L magnesium;2-aminobutanedioate Chemical compound [Mg+2].[O-]C(=O)C(N)CC([O-])=O NFFJLMKHRCXLJO-UHFFFAOYSA-L 0.000 claims description 3
- 239000011656 manganese carbonate Substances 0.000 claims description 3
- 235000006748 manganese carbonate Nutrition 0.000 claims description 3
- 229940093474 manganese carbonate Drugs 0.000 claims description 3
- 239000011565 manganese chloride Substances 0.000 claims description 3
- 235000002867 manganese chloride Nutrition 0.000 claims description 3
- 229940099607 manganese chloride Drugs 0.000 claims description 3
- 229940099596 manganese sulfate Drugs 0.000 claims description 3
- 239000011702 manganese sulphate Substances 0.000 claims description 3
- 235000007079 manganese sulphate Nutrition 0.000 claims description 3
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 claims description 3
- 229910000016 manganese(II) carbonate Inorganic materials 0.000 claims description 3
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 3
- XMWCXZJXESXBBY-UHFFFAOYSA-L manganese(ii) carbonate Chemical compound [Mn+2].[O-]C([O-])=O XMWCXZJXESXBBY-UHFFFAOYSA-L 0.000 claims description 3
- 235000013337 tricalcium citrate Nutrition 0.000 claims description 3
- PLSARIKBYIPYPF-UHFFFAOYSA-H trimagnesium dicitrate Chemical compound [Mg+2].[Mg+2].[Mg+2].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O PLSARIKBYIPYPF-UHFFFAOYSA-H 0.000 claims description 3
- 239000004246 zinc acetate Substances 0.000 claims description 3
- 239000011592 zinc chloride Substances 0.000 claims description 3
- 235000005074 zinc chloride Nutrition 0.000 claims description 3
- 239000011576 zinc lactate Substances 0.000 claims description 3
- 235000000193 zinc lactate Nutrition 0.000 claims description 3
- 229940050168 zinc lactate Drugs 0.000 claims description 3
- 239000011787 zinc oxide Substances 0.000 claims description 3
- OWVLYQRCCIEOPF-QHTZZOMLSA-L zinc;(2s)-5-oxopyrrolidine-2-carboxylate Chemical compound [Zn+2].[O-]C(=O)[C@@H]1CCC(=O)N1.[O-]C(=O)[C@@H]1CCC(=O)N1 OWVLYQRCCIEOPF-QHTZZOMLSA-L 0.000 claims description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 2
- 235000010376 calcium ascorbate Nutrition 0.000 claims description 2
- 229940047036 calcium ascorbate Drugs 0.000 claims description 2
- 239000011692 calcium ascorbate Substances 0.000 claims description 2
- BLORRZQTHNGFTI-ZZMNMWMASA-L calcium-L-ascorbate Chemical compound [Ca+2].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] BLORRZQTHNGFTI-ZZMNMWMASA-L 0.000 claims description 2
- 239000010949 copper Substances 0.000 claims description 2
- 229910052802 copper Inorganic materials 0.000 claims description 2
- MFGZXPGKKJMZIY-UHFFFAOYSA-N ethyl 5-amino-1-(4-sulfamoylphenyl)pyrazole-4-carboxylate Chemical compound NC1=C(C(=O)OCC)C=NN1C1=CC=C(S(N)(=O)=O)C=C1 MFGZXPGKKJMZIY-UHFFFAOYSA-N 0.000 claims description 2
- 238000004108 freeze drying Methods 0.000 claims description 2
- FBAFATDZDUQKNH-UHFFFAOYSA-M iron chloride Chemical compound [Cl-].[Fe] FBAFATDZDUQKNH-UHFFFAOYSA-M 0.000 claims description 2
- RKTIGKJUTHIMNR-UAIGZDOSSA-L magnesium;(2s)-2-acetamido-4-methylsulfanylbutanoate Chemical class [Mg+2].CSCC[C@@H](C([O-])=O)NC(C)=O.CSCC[C@@H](C([O-])=O)NC(C)=O RKTIGKJUTHIMNR-UAIGZDOSSA-L 0.000 claims description 2
- 238000001694 spray drying Methods 0.000 claims description 2
- 239000011670 zinc gluconate Substances 0.000 claims description 2
- 235000011478 zinc gluconate Nutrition 0.000 claims description 2
- 229960000306 zinc gluconate Drugs 0.000 claims description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims 2
- 244000248349 Citrus limon Species 0.000 claims 1
- 235000005979 Citrus limon Nutrition 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 229960002449 glycine Drugs 0.000 claims 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims 1
- 239000010931 gold Substances 0.000 claims 1
- 229910052737 gold Inorganic materials 0.000 claims 1
- 150000003233 pyrroles Chemical class 0.000 claims 1
- 229940079593 drug Drugs 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 8
- 235000018102 proteins Nutrition 0.000 description 8
- 102000004169 proteins and genes Human genes 0.000 description 8
- 108090000623 proteins and genes Proteins 0.000 description 8
- 239000000843 powder Substances 0.000 description 6
- 230000008901 benefit Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000014759 maintenance of location Effects 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 241000040710 Chela Species 0.000 description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 235000010331 calcium propionate Nutrition 0.000 description 2
- 239000004330 calcium propionate Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000010612 desalination reaction Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000011790 ferrous sulphate Substances 0.000 description 2
- 235000003891 ferrous sulphate Nutrition 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 2
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000003334 potential effect Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 210000004885 white matter Anatomy 0.000 description 2
- ASJSAQIRZKANQN-CRCLSJGQSA-N 2-deoxy-D-ribose Chemical group OC[C@@H](O)[C@@H](O)CC=O ASJSAQIRZKANQN-CRCLSJGQSA-N 0.000 description 1
- 108010080981 3-phytase Proteins 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 241000613162 Craspedacusta sowerbyi Species 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- 240000000203 Salix gracilistyla Species 0.000 description 1
- 101710109488 Salt stress-induced protein Proteins 0.000 description 1
- 102100025292 Stress-induced-phosphoprotein 1 Human genes 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 235000017168 chlorine Nutrition 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- GTAKGKCNJQLSMO-UHFFFAOYSA-N copper 5-oxopyrrolidine-2-carboxylic acid Chemical compound [Cu].OC(=O)C1CCC(=O)N1 GTAKGKCNJQLSMO-UHFFFAOYSA-N 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 159000000003 magnesium salts Chemical class 0.000 description 1
- GHEUCKDZCFXJGC-UHFFFAOYSA-N magnesium;5-oxopyrrolidine-2-carboxylic acid Chemical compound [Mg].OC(=O)C1CCC(=O)N1 GHEUCKDZCFXJGC-UHFFFAOYSA-N 0.000 description 1
- 229940099594 manganese dioxide Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 229940043825 zinc carbonate Drugs 0.000 description 1
- 229960001296 zinc oxide Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/04—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from fish or other sea animals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Abstract
The invention discloses a kind of polypeptide in aurelia source and its preparation process and applications, prepare in accordance with the following steps: (1) taking fresh or freezing aurelia, cleaned with seawater or deionized water, put into reaction kettle, chelating agent and metal salt is added;(2) it at 1-10 DEG C, opens agitating paddle and blends aurelia, until the g., jelly-like particle being visible by naked eyes in mixture, homogeneous;(3) mixture is filtered, retains filter residue, is washed with deionized water;(4) clean filter residue is put into enzyme reaction kettle, deionized water and protease is added, reacts 2-10h at 35-60 DEG C, is then heated to 90-100 DEG C, keep 10-20min, inactivation;(5) it is cooled to 35-60 DEG C, suitable chelating agent enzyme is added, reacts 2-10h, is then heated to 90-100 DEG C, keeps 10-20min, inactivation;(6) it is cooled to room temperature, precipitating reagent is added into mixture, stirs 10-30min, filtering collects filtrate, is drying to obtain aurelia polypeptide.It can be widely applied in the fields such as cosmetics, health care product, drug.
Description
Technical field
The present invention relates to a kind of polypeptides and its preparation method and application in aurelia source, belong to marine biology technology
Field.
Background technique
Before jellyfish comes across 6.5 hundred million years, after 5 secondary pollutant mass extinctions, still actively so far, illustrate that it resists cruel ring
The ability and self-regeneration function in border are very powerful.In addition jellyfish excessive multiplication, to sea fishery, marine ecology, ocean periphery
Industry produces more serious harm, so the development and utilization to it are particularly important.The water content of jellyfish is generally 96%
More than, in remaining non-aqueous ingredient, content is more for protein, current application study hot spot all concentrate on jellyfish (such as jellyfish,
Sand bites, rosy clouds jellyfish, craspedacusta sowerbyi, fire medusa, it is celestial after jellyfish) protein and peptide class extraction and application it is upper.
But there is a kind of jellyfish ignored, its research rests in ornamental value always, it is exactly aurelia.Sea
Month jellyfish is colorless and transparent, without other variegated, only lives in the high sea area of water quality, is the smallest jellyfish of toxicity, is to extract egg
The ideal material of white matter and its derivative.But water content is big, easily self-dissolving, it is more difficult to save, marinated rear desalination dealuminzation difficulty etc. is asked
Topic, restricts always the deep exploitation of aurelia, also results in vast resources waste.
Summary of the invention
To solve the above-mentioned problems, the object of the present invention is to provide a kind of preparation process of the polypeptide in aurelia source and
The polypeptide in aurelia source, natural, highly-safe, high income, moisture retention are strong.The present invention also provides aurelia sources
The application of polypeptide.
For up to above-mentioned first purpose, the present invention provides a kind of preparation process of the polypeptide in aurelia source, features
It is, comprising the following steps:
(1) fresh or freezing aurelia is taken, is cleaned with seawater or deionized water, puts into reaction kettle, chelating agent is added
And metal salt;
(2) it at 1-10 DEG C, opens agitating paddle and blends aurelia, until the g., jelly-like being visible by naked eyes in mixture
Grain, homogeneous;
(3) mixture is filtered, retains filter residue, is washed with deionized water;
(4) clean filter residue is put into enzyme reaction kettle, deionized water and protease is added, reacts 2-10h at 35-60 DEG C,
It is then heated to 90-100 DEG C, keeps 10-20min, inactivation;
(5) it is cooled to 35-60 DEG C, chelating agent enzyme is added, reacts 2-10h, is then heated to 90-100 DEG C, keeps 10-
20min, inactivation;
(6) it is cooled to room temperature, precipitating reagent is added into mixture, stirs 10-30min, filtrate is collected in filtering, and drying is
Obtain aurelia polypeptide.
Then the present invention uses protease by solidifying the protein in precipitating aurelia with chelating agent and metal salt
Protein is digested, the polypeptide chelated, then fallen chelating agent enzymatic hydrolysis with chelating agent enzyme, released polypeptide, finally use
Precipitating reagent removes the Partial digestion object of chelating agent, finally that enzymolysis liquid is dry, obtain a kind of natural, highly-safe, high income,
The polypeptide in strong, with a variety of potential activities the aurelia source of moisture retention.Obtain aurelia ignored in jellyfish group
The utilization of scientific research level is arrived.
In above scheme: chelating agent described in step (1) and metal salt are natural chelating agent and natural
Metal salt.It is preferred that the natural origin chelating agent is at least one of phytic acid, tannic acid, ellagic acid, additive amount
It is the 0.002~0.1% of aurelia quality;The natural origin metal salt is DNA calcium, deoxyribose core
Sour magnesium, DNA zinc, 2-pyrrolidone-5-carboxylic acid's calcium, 2-pyrrolidone-5-carboxylic acid's magnesium, 2-pyrrolidone-5-carboxylic acid's manganese, 2-pyrrolidone-5-carboxylic acid
Copper, zinc pyrrolidone carboxylate, calcium propionate, calcium pantothenate, glycine zine, Calcium Ascorbate, Magnesium ascorbate, magnesium sulfate, manganese sulfate,
Copper sulphate, zinc sulfate, ferrous sulfate, calcium chloride, magnesium chloride, manganese chloride, iron chloride, copper chloride, zinc chloride, calcium citrate, lemon
Lemon acid magnesium, Titanium Citrate, ironic citrate, calcium gluconate, gluconic acid cobalt, gluconic acid magnesium, managanese gluconate, copper gluconate, gluconic acid
Zinc, ferrous gluconate, calcium lactate, magnesium lactate, zinc lactate, magnesium salicylate, zinc salicylate, calcium aspartate, magnesium aminosuccinate, day
Aspartic acid manganese salt, aspartic acid copper, aspartic acid zinc, magnesium nitrate, calcium acetate, magnesium acetate, copper acetate, zinc acetate, acetyl egg ammonia
At least one of sour magnesium salts, Acetylmethionine copper, Acetylmethionine zinc, additive amount be aurelia quality 0.002~
0.1%.The chelating agent of selection is good to the chelate effect of the protein in aurelia, the metal salt solidification precipitating Hai Yueshui of selection
The effect of parent protein is good, and safe and reliable.
In above scheme: the additive amount of the deionized water in step (4) is 0.5-2 times of aurelia quality;Protease
For at least one of acid protease, neutral proteinase, alkali protease, additive amount be aurelia quality 0.02~
0.1%.The hydrolysis result well to protein can be played.
In above scheme: chelating agent enzyme described in step (5) be at least one of phytase, tannase, ellagic acid enzyme,
Its additive amount is the 0.02~0.1% of aurelia quality.Chelating agent hydrolysis result is good.
In above scheme: precipitating reagent is natural origin precipitating reagent in step (6), and the natural origin precipitating reagent is oxidation
Calcium, calcium carbonate, calcium hydroxide, magnesia, magnesium carbonate, magnesium hydroxide, basic magnesium carbonate, zinc oxide, zinc carbonate, manganese dioxide,
At least one of manganese carbonate, ferrous oxide, iron oxide, additive amount are the 0.002~0.1% of aurelia quality.To chela
The zymolyte removal effect of mixture is good, and safe and reliable.
In above scheme: the drying mode in step (6) is at least one of freeze-drying, spray drying.
The second object of the present invention is achieved in that a kind of polypeptide in aurelia source prepared by the above method.
In above scheme: the relative molecular mass of the polypeptide is 300-5000.
Application of the polypeptide in the aurelia source in cosmetics, health care product, medicine field.
The invention has the following advantages:
(1) present invention prepares polypeptide using aurelia often ignored in jellyfish group, has filled up technological gap.
(2) aurelia derives from free of contamination sea area, abundant raw material, and scientific utilization aurelia both meets national marine
It makes the country prosperous strategy, is also advantageous for marine ecology, and advantage of lower cost.
(3) prepare polypeptide using edible aurelia and natural auxiliary agent, ensure that polypeptide safety and
Natural sex;Natural origin chelating agent and natural origin metal salt are added in extraction process, chelates and precipitates the egg in aurelia
White matter protects the active site on protein, efficiently separating for protein and jellyfish body fluid is realized, using protease to chela
The exposed sites of hop protein matter are digested, and the peptides with specific function can be extracted;It is more to chelating using chelating agent enzyme
Peptide is digested, and target polypeptides are released;Using natural origin precipitating reagent, the Partial digestion product of chelating agent is removed, improves mesh
Mark the purity of polypeptide.
(4) preparation method is simple, using fresh and freezing aurelia as raw material, eliminates lengthy and tedious marinated step and desalination
Dealuminzation step, also saves water resource;It is easy to industrialized production, it improves resource utilization.
The polypeptide in aurelia source of the invention is entirely derived from naturally, and with high security, moisture retention is strong, yield
It is high, skin-friendly is strong, has many advantages, such as a variety of potential activities, and its cost is relatively low, preparation method is simple, environmental-friendly, is easy to work
Industry metaplasia produces, and can be widely applied in the fields such as cosmetics, health care product, drug.
Specific embodiment
Below with reference to embodiment, the invention will be further described.It should be understood that following embodiment is merely to illustrate the present invention
Range and is not intended to limit the present invention.
Embodiment 1:
A kind of preparation method of the polypeptide in aurelia source, its step are as follows:
(1) fresh aurelia 1000g is taken, is cleaned with seawater, reaction kettle is put into, the phytic acid of 0.02g is added
With 0.03g calcium chloride.
(2) it is cooled to 1-2 DEG C, agitating paddle is opened and blends aurelia, until the g., jelly-like being visible by naked eyes in mixture
Particle, then homogeneous 10min.
(3) mixture is filtered, retains filter residue, is washed with deionized water.
(4) filter residue that will be clean puts into enzyme reaction kettle, is added 500g deionized water and 0.2g neutral proteinase, anti-at 37 DEG C
3h is answered, is then heated to 100 DEG C, keeps 10min, inactivation.
(5) 40 DEG C are cooled to, 0.2g phytase is added, reacts 3h, is then heated to 100 DEG C, keeps 10min, inactivation.
(6) it is cooled to room temperature, 0.02g calcium carbonate is added into mixture, stirs 10min, filtrate is collected in filtering, dry
Up to aurelia polypeptide, it is sealed in a cool and dry place.
The polypeptide in the aurelia source prepared according to the method described above is white powder, obtains 1.92g, the aurelia
Polypeptide can be directly added into water phase, be added in moisturiser, it is proposed that additive amount 0.1~0.5%.
Embodiment 2:
A kind of preparation method of the polypeptide in aurelia source, its step are as follows:
(1) fresh aurelia 1000g is taken, is washed with deionized water, reaction kettle is put into, the inositol six of 0.03g is added
Phosphoric acid and 0.05g magnesium citrate.
(2) it is cooled to 1-4 DEG C, agitating paddle is opened and blends aurelia, until the g., jelly-like being visible by naked eyes in mixture
Particle, then homogeneous 15min.
(3) mixture is filtered, retains filter residue, is washed with deionized water.
(4) filter residue that will be clean puts into enzyme reaction kettle, is added 1000g deionized water and 0.3g neutral proteinase, at 40 DEG C
5h is reacted, is then heated to 100 DEG C, keeps 10min, inactivation.
(5) 35 DEG C are cooled to, 0.3 phytase is added, reacts 5h, is then heated to 100 DEG C, keeps 10min, inactivation.
(6) it is cooled to room temperature, 0.04g zinc carbonate is added into mixture, stirs 15min, filtrate is collected in filtering, dry
Up to aurelia polypeptide, it is sealed in a cool and dry place.
The polypeptide in the aurelia source prepared according to the method described above is white powder, obtains 2.16g, the aurelia
Polypeptide can be directly added into water phase, be added in essence lotion, it is proposed that additive amount 0.05~0.3%.
Embodiment 3:
A kind of preparation method of the polypeptide in aurelia source, its step are as follows:
(1) fresh aurelia 2000g is taken, is cleaned with seawater, reaction kettle is put into, the tannic acid and 0.06g of 0.05g is added
Zinc sulfate.
(2) 1-5 DEG C is cooled to, agitating paddle is opened and blends aurelia, until the g., jelly-like being visible by naked eyes in mixture
Particle, then homogeneous 20min.
(3) mixture is filtered, retains filter residue, is washed with deionized water.
(4) filter residue that will be clean puts into enzyme reaction kettle, is added 1500g deionized water and 0.5g acid protease, at 38 DEG C
8h is reacted, is then heated to 90 DEG C, keeps 20min, inactivation.
(5) 42 DEG C are cooled to, 0.2g tannase is added, reacts 4h, is then heated to 90 DEG C, keeps 20min, inactivation.
(6) it is cooled to room temperature, 0.06g calcium oxide is added into mixture, stirs 20min, filtrate is collected in filtering, dry
Up to aurelia polypeptide, it is sealed in a cool and dry place.
The polypeptide in the aurelia source prepared according to the method described above is pale yellow powder, obtains 4.07g, the Hai Yueshui
Female polypeptide can be directly added into water phase, be added in skin care milk, it is proposed that additive amount 0.1~0.5%.
Embodiment 4:
A kind of preparation method of the polypeptide in aurelia source, its step are as follows:
(1) fresh aurelia 500g is taken, is cleaned with seawater, reaction kettle is put into, the tannic acid and 0.03g of 0.02g is added
Calcium acetate;
(2) it is cooled to 1-8 DEG C, agitating paddle is opened and blends aurelia, until the g., jelly-like being visible by naked eyes in mixture
Particle, then homogeneous 15min.
(3) mixture is filtered, retains filter residue, is washed with deionized water.
(4) filter residue that will be clean puts into enzyme reaction kettle, is added 1000g deionized water and 0.2g acid protease, at 40 DEG C
3h is reacted, is then heated to 100 DEG C, keeps 15min, inactivation.
(5) 45 DEG C are cooled to, 0.15g tannase is added, reacts 5h, is then heated to 100 DEG C, keeps 15min, inactivation.
(6) it is cooled to room temperature, 0.02g basic magnesium carbonate is added into mixture, stirs 20min, filtrate is collected in filtering,
It is drying to obtain aurelia polypeptide, is sealed in a cool and dry place.
The polypeptide in the aurelia source prepared according to the method described above is pale yellow powder, obtains 0.96g, the Hai Yueshui
Female polypeptide can be directly added into water phase, be added in handguard gel, it is proposed that additive amount 0.1~0.3%.
Embodiment 5:
A kind of preparation method of the polypeptide in aurelia source, its step are as follows:
(1) the aurelia 5000g for taking freezing is cleaned with seawater, puts into reaction kettle, and 5g tannic acid and 5g aspartic acid is added
Zinc.
(2) it at 1-10 DEG C of low temperature, opens agitating paddle and blends aurelia, until the g., jelly-like being visible by naked eyes in mixture
Particle, then homogeneous 30min.
(3) mixture is filtered, retains filter residue, is washed with deionized water.
(4) filter residue that will be clean puts into enzyme reaction kettle, is added 10000g deionized water and 5g alkali protease, anti-at 35 DEG C
10h is answered, is then heated to 100 DEG C, keeps 20min, inactivation.
(5) 35 DEG C are cooled to, the ellagic acid enzyme of 5g is added, reacts 10h, is then heated to 100 DEG C, 20min is kept, goes out
It is living.
(6) it is cooled to room temperature, 5g basic magnesium carbonate is added into mixture, stirs 30min, filtrate is collected in filtering, dry
Up to aurelia polypeptide, it is sealed in a cool and dry place.
The polypeptide in the aurelia source prepared according to the method described above is pale yellow powder, obtains 108.9g, the Hai Yueshui
Female polypeptide can be directly added into water phase, be added in handguard gel, it is proposed that additive amount 0.1~0.3%.
Embodiment 6:
A kind of preparation method of the polypeptide in aurelia source, its step are as follows:
(1) the aurelia 5000g for taking freezing is cleaned with seawater, puts into reaction kettle, and 2g ellagic acid and 1.6g propionic acid is added
Calcium.
(2) it at 1-10 DEG C of low temperature, opens agitating paddle and blends aurelia, until the g., jelly-like being visible by naked eyes in mixture
Particle, then homogeneous 30min.
(3) mixture is filtered, retains filter residue, is washed with deionized water.
(4) filter residue that will be clean puts into enzyme reaction kettle, is added 5000g deionized water and 3g alkali protease, anti-at 60 DEG C
2h is answered, is then heated to 100 DEG C, keeps 20min, inactivation.
(5) 60 DEG C are cooled to, the ellagic acid enzyme of 4g is added, reacts 2h, is then heated to 100 DEG C, keeps 20min, inactivation.
(6) it is cooled to room temperature, 3g magnesium carbonate is added into mixture, stirs 30min, filtering is collected filtrate, is drying to obtain
Aurelia polypeptide, is sealed in a cool and dry place.
The polypeptide in the aurelia source prepared according to the method described above is pale yellow powder, obtains 114.8g, the Hai Yueshui
Female polypeptide can be directly added into water phase, be added in handguard gel, it is proposed that additive amount 0.1~0.3%.
Although an embodiment of the present invention has been shown and described, it will be understood by those skilled in the art that: not
A variety of change, modification, replacement and modification can be carried out to these embodiments in the case where being detached from the principle of the present invention and objective, such as
The natural origin chelating agent can be one of phytic acid, tannic acid, ellagic acid or combination.Natural origin metal
Salt is DNA calcium, DNA magnesium, DNA zinc, 2-pyrrolidone-5-carboxylic acid's calcium, 2-pyrrolidone-5-carboxylic acid
Magnesium, 2-pyrrolidone-5-carboxylic acid's manganese, 2-pyrrolidone-5-carboxylic acid's copper, zinc pyrrolidone carboxylate, calcium propionate, calcium pantothenate, glycine zine, Vitamin C
Sour calcium, Magnesium ascorbate, magnesium sulfate, manganese sulfate, copper sulphate, zinc sulfate, ferrous sulfate, calcium chloride, magnesium chloride, manganese chloride, chlorine
Change iron, copper chloride, zinc chloride, calcium citrate, magnesium citrate, Titanium Citrate, ironic citrate, calcium gluconate, gluconic acid cobalt, glucose
Sour magnesium, managanese gluconate, copper gluconate, zinc gluconate, ferrous gluconate, calcium lactate, magnesium lactate, zinc lactate, magnesium salicylate, bigcatkin willow
Sour zinc, calcium aspartate, magnesium aminosuccinate, aspartic acid manganese salt, aspartic acid copper, aspartic acid zinc, magnesium nitrate, calcium acetate,
One or more of magnesium acetate, copper acetate, zinc acetate, Acetylmethionine magnesium salts, Acetylmethionine copper, Acetylmethionine zinc
Combination.
Protease is one of acid protease, neutral proteinase, alkali protease or any combination.
Chelating agent enzyme is one of phytase, tannase, ellagic acid enzyme or any combination.
Natural origin precipitating reagent is calcium oxide, calcium carbonate, calcium hydroxide, magnesia, magnesium carbonate, magnesium hydroxide, alkali formula carbon
One of sour magnesium, zinc oxide, zinc carbonate, manganese dioxide, manganese carbonate, ferrous oxide, iron oxide or combination.The present invention
Range be defined by the claims and their equivalents.
Claims (10)
1. a kind of preparation process of the polypeptide in aurelia source, which comprises the following steps:
(1) fresh or freezing aurelia is taken, is cleaned with seawater or deionized water, puts into reaction kettle, chelating agent and gold is added
Belong to salt;
(2) it at 1-10 DEG C, opens agitating paddle and blends aurelia, until the g., jelly-like particle being visible by naked eyes in mixture,
Homogeneous;
(3) mixture is filtered, retains filter residue, is washed with deionized water;
(4) clean filter residue is put into enzyme reaction kettle, deionized water and protease is added, reacts 2-10h at 35-60 DEG C, then
It is heated to 90-100 DEG C, keeps 10-20min, inactivation;
(5) it is cooled to 35-60 DEG C, chelating agent enzyme is added, reacts 2-10h, is then heated to 90-100 DEG C, keeps 10-20min,
Inactivation;
(6) it is cooled to room temperature, precipitating reagent is added into mixture, stirs 10-30min, filtering collects filtrate, is drying to obtain sea
Month jellyfish polypeptide.
2. the preparation process of the polypeptide in aurelia source according to claim 1, it is characterised in that: described in step (1)
Chelating agent and metal salt are natural chelating agent and natural metal salt.
3. the preparation process of the polypeptide in aurelia source according to claim 2, it is characterised in that: the natural origin
Chelating agent be at least one of phytic acid, tannic acid, ellagic acid, additive amount be aurelia quality 0.002~
0.1%;The natural origin metal salt is DNA calcium, DNA magnesium, DNA zinc, pyrroles
Alkanone calcium carboxylates, 2-pyrrolidone-5-carboxylic acid's magnesium, 2-pyrrolidone-5-carboxylic acid's manganese, 2-pyrrolidone-5-carboxylic acid's copper, zinc pyrrolidone carboxylate, propionic acid
Calcium, calcium pantothenate, glycine zine, Calcium Ascorbate, Magnesium ascorbate, magnesium sulfate, manganese sulfate, copper sulphate, zinc sulfate, sulfuric acid are sub-
Iron, calcium chloride, magnesium chloride, manganese chloride, iron chloride, copper chloride, zinc chloride, calcium citrate, magnesium citrate, Titanium Citrate, lemon
Sour iron, calcium gluconate, gluconic acid cobalt, gluconic acid magnesium, managanese gluconate, copper gluconate, zinc gluconate, ferrous gluconate, calcium lactate,
Magnesium lactate, zinc lactate, magnesium salicylate, zinc salicylate, calcium aspartate, magnesium aminosuccinate, aspartic acid manganese salt, aspartic acid
Copper, aspartic acid zinc, magnesium nitrate, calcium acetate, magnesium acetate, copper acetate, zinc acetate, Acetylmethionine magnesium salts, Acetylmethionine copper,
At least one of Acetylmethionine zinc, additive amount are the 0.002~0.1% of aurelia quality.
4. the preparation process of the polypeptide in any one of -3 aurelia sources according to claim 1, it is characterised in that: step
(4) additive amount of the deionized water in is 0.5-2 times of aurelia quality;Protease be acid protease, neutral proteinase,
At least one of alkali protease, additive amount are the 0.02~0.1% of aurelia quality.
5. the preparation process of the polypeptide in aurelia source according to claim 4, it is characterised in that: described in step (5)
Chelating agent enzyme be at least one of phytase, tannase, ellagic acid enzyme, additive amount be aurelia quality 0.02~
0.1%.
6. the preparation process of the polypeptide in aurelia source according to claim 5, it is characterised in that: precipitating in step (6)
Agent be natural origin precipitating reagent, the natural origin precipitating reagent be calcium oxide, calcium carbonate, calcium hydroxide, magnesia, magnesium carbonate,
Magnesium hydroxide, basic magnesium carbonate, zinc oxide, zinc carbonate, manganese dioxide, manganese carbonate, ferrous oxide, at least one in iron oxide
Kind, additive amount is the 0.002~0.1% of aurelia quality.
7. the preparation process of the polypeptide in aurelia source according to claim 6, it is characterised in that: in step (6)
Drying mode is at least one of freeze-drying, spray drying.
8. a kind of polypeptide in aurelia source prepared by any one of claim 1-7.
9. the polypeptide in aurelia source according to claim 8, which is characterized in that the relative molecular mass of the polypeptide is
300-5000。
10. polypeptide the answering in cosmetics, health care product, medicine field in the aurelia source any one of claim 8-9
With.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN111011575A (en) * | 2019-12-30 | 2020-04-17 | 广州市科能化妆品科研有限公司 | Jellyfish growth promoting peptide and preparation method thereof |
CN111437242A (en) * | 2020-05-07 | 2020-07-24 | 江苏华能药业有限公司 | Crystal mask prepared from jellyfish |
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US20110053837A1 (en) * | 2009-09-01 | 2011-03-03 | Nugen Bioscience (Taiwan) Co., Ltd. | Mineral-peptide chelates |
CN102191306A (en) * | 2011-04-09 | 2011-09-21 | 山东好当家海洋发展股份有限公司 | Enzymatic preparation method for antihypertensive peptides from jellyfish |
CN102511890A (en) * | 2012-01-07 | 2012-06-27 | 中国海洋大学 | Jellyfish beverage |
CN105767452A (en) * | 2016-03-07 | 2016-07-20 | 湖北工业大学 | Manufacture method of sacha inchi polypeptide |
CN107586332A (en) * | 2017-09-20 | 2018-01-16 | 青岛海洋生物医药研究院股份有限公司 | A kind of active low molecule amount jellyfish collagen peptide and its preparation method and application |
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2018
- 2018-12-20 CN CN201811560639.XA patent/CN109548950A/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
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US20110053837A1 (en) * | 2009-09-01 | 2011-03-03 | Nugen Bioscience (Taiwan) Co., Ltd. | Mineral-peptide chelates |
CN102191306A (en) * | 2011-04-09 | 2011-09-21 | 山东好当家海洋发展股份有限公司 | Enzymatic preparation method for antihypertensive peptides from jellyfish |
CN102511890A (en) * | 2012-01-07 | 2012-06-27 | 中国海洋大学 | Jellyfish beverage |
CN105767452A (en) * | 2016-03-07 | 2016-07-20 | 湖北工业大学 | Manufacture method of sacha inchi polypeptide |
CN107586332A (en) * | 2017-09-20 | 2018-01-16 | 青岛海洋生物医药研究院股份有限公司 | A kind of active low molecule amount jellyfish collagen peptide and its preparation method and application |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN111011575A (en) * | 2019-12-30 | 2020-04-17 | 广州市科能化妆品科研有限公司 | Jellyfish growth promoting peptide and preparation method thereof |
CN111011575B (en) * | 2019-12-30 | 2023-03-31 | 广州市科能化妆品科研有限公司 | Jellyfish growth promoting peptide and preparation method thereof |
CN111437242A (en) * | 2020-05-07 | 2020-07-24 | 江苏华能药业有限公司 | Crystal mask prepared from jellyfish |
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