CN109548651A - Wild gold lotus quick breeding propagation method - Google Patents
Wild gold lotus quick breeding propagation method Download PDFInfo
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- CN109548651A CN109548651A CN201811265662.6A CN201811265662A CN109548651A CN 109548651 A CN109548651 A CN 109548651A CN 201811265662 A CN201811265662 A CN 201811265662A CN 109548651 A CN109548651 A CN 109548651A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
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Abstract
The invention discloses wild gold lotus quick breeding propagation method, method includes the following steps: a, picking fruit, with thiocarbamide and chlorethanol cold soaking 3 days, and it is more than half an hour to mix disinfection with ethyl alcohol with hydrogen peroxide；B, it prepares with MS, niacin, folic acid, puridoxine hydrochloride, cobalamin, agar, fructose, creatinine, acid hydrolyzed casein, cell division, carries out aseptic seeding；C, seed sprouts protocorm after 6 months, and protocorm further breaks up rhizomes, can sprout seedling out from rhizomes；D, can largely transfer proliferated culture medium on minimal medium: basal medium, banana puree, fructose, active carbon, grape juice, corn juice, yeast extract；E, when seeing that seedling occurs in tissue culture bottle after 5-6 months, seedling is moved in 6000lux-8000lux environment greenhouse, is planted with fertile soil, exogenous nutrition substance is applied, it is promoted to take root；The present invention plants based on fertile soil, sprays exogenous nutrition substance, it is promoted to take root, and transplanting survival rate is up to 90%.
Description
Technical field
The present invention relates to wild gold lotus quick breeding propagation methods.
Background technique
Roxburgh anoectochilus terminal bud (also known as Shorthairy Antenoron) is orchid family Anoectochilus Blume plant.There are Herba Anoectochili roxburghii, gold ear ring, bird ginseng, gold civil
Line taiwan anetochilus herb, gold thread disappear to the marrow, herba lysimachiae, the laudatory titles such as gold thread lycopod.It is mainly characterized by comprising: leaf buckler-shaped, rhizome is thinner, and petiole is thin
Long, sepia can climb up by holding on to.It can not resist cold, like warm and moist, 10 DEG C of overwintering temperature or more.Peanut has yellow, red, reddish brown, newborn in axil
White equal colors, it is irregular.
The content of both amino acid and microelement is above domestic American Ginseng and wild ginseng in roxburgh anoectochilus terminal bud；It and ox sulphur
Acid, polysaccharide component have the function of nutrition, anti-aging, adjust human body immunity of organism.Roxburgh anoectochilus terminal bud is that work fatigue person health care is first
Select natural beverage: roxburgh anoectochilus terminal bud can enter kidney, the heart, lung three and pass through, there is reconciliation the five internal organs, liver protecting, relieve the effect of alcohol and other effects.It can improve comprehensively
Body immunity enhances human body to the resistance of disease, and roxburgh anoectochilus terminal bud is to bronchitis, ephritis, cystitis, diabetes, blood urine, wind
Wet arthritis, acute and chronic hepatitis, hypertension, artery sclerosis, cerebral thrombosis etc. have auxiliary effect, and the normal Baoshang of roxburgh anoectochilus terminal bud is drunk,
Energy toxin-expelling and face nourishing anti-aging adjusts endocrine, the regulation of qi and blood, beauty treatment of promoting the production of body fluid, and drinks roxburgh anoectochilus terminal bud for a period of time, can effectively eliminate blueness
Spring acne and acne.
The cultivation technique of existing roxburgh anoectochilus terminal bud is all that preferred wild gold lotus is transplanted to artificial culture stage, culture medium
Supply be subsequent product quality and yield can not or scarce guarantee, the medical value of roxburgh anoectochilus terminal bud increasingly approved by market,
Therefore, what the ectogenesis stage of early period became is most important, so needing a kind of complete using wild gold lotus fastly in the market
Fast propagation method.
Summary of the invention
Roxburgh anoectochilus terminal bud can be effectively facilitated technical problem to be solved by the invention is to provide one kind to take root, and improve transplanting survival rate
Wild gold lotus rapid propagation method.
To solve the above problems, the present invention is achieved through the following technical solutions: wild gold lotus quick breeding is numerous
Grow method, comprising the following steps:
A, the roxburgh anoectochilus terminal bud wild fruit for harvesting not instant of complete cracking, with the thiocarbamide and chlorethanol of 150mg/L with the ratio of 1:1
Example mixed liquor cold soaking 3 days, and it is more than half an hour to mix disinfection with 75% ethyl alcohol with 2000mg/L hydrogen peroxide, with distillation after taking-up
Water carefully rinses at least 2 times；
B, it prepares with MS 0.2-0.3mg/L, niacin 0.2-0.3mg/L, folic acid 0.2-0.3mg/L, puridoxine hydrochloride 0.3-
0.5mg/L, cobalamin 0.1-0.3mg/L, agar 1-2g/L, fructose 8-11g/L, creatinine 30-50mg/L, acid hydrolyzed casein
100-120mg/L, basic element of cell division 0.5-1mg/L carry out aseptic seeding；
C, seed sprouts the protocorm for being initially formed green after 6 months, and protocorm is further differentiated to form the rhizomes of green,
There are multiple growing points on rhizomes, seedling out can be sprouted from the growing point of rhizomes；
D, can largely transfer proliferated culture medium on minimal medium, culture medium prescription: 1/2M basal medium, banana
Mud 15-20g/L, fructose 5-10g/L, active carbon 0.1-0.5g/L, grape juice 5-8g/L, corn juice 10-15g/L, yeast extract
Object 10-30mg/L；
E, when seeing that blade-carrying a large amount of seedling occur in tissue culture bottle after 5-6 months, by seedling from 24-27 DEG C of constant humidity of constant temperature
The environment of 30-45% moves in 6000lux-8000lux environment greenhouse, is planted with fertile soil, applies exogenous nutrition substance, promotes
It is taken root.
Further, in stepb, the basic element of cell division is that dihydro zeatin and 6- benzylaminopurine are matched with 3:1
Than mixing.
Further, in step d, the grape juice is prepared as taking new fresh grape, removes its skin, the boiling in distilled water
20 minutes, then used filter mesh sieve filtered its coarse stem item, and the corn juice is placed on small in distilled water with fresh corn grain
Fire boils 30 minutes, takes out particle and is squeezed, the juice squeezed out is mixed with distilled water 2:1
The beneficial effects of the present invention are: the present invention based on fertile soil, sprays exogenous nutrition substance, it is promoted to take root, moved
Survival rate is planted up to 90% or more, meanwhile, this method can effectively shorten the training seedling period, be down to 6 to 8 months from nearly 1 year, become
In 1-3%, seedling uniformity and rooting rate are relatively high, and growth and development is outstanding between root and seedling, do not find bright for different rate control
Aobvious callus restores good, and front and back culture medium can preferably guarantee it with the use of seedling percent can be greatly improved
Domestication degree brings many economics well-being of workers and staff to enterprise or peasant household, reduces cost of investment.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..
Embodiment 1
A, the roxburgh anoectochilus terminal bud wild fruit for harvesting not instant of complete cracking, with the thiocarbamide and chlorethanol of 150mg/L with the ratio of 1:1
Example mixed liquor cold soaking 3 days, and it is more than half an hour to mix disinfection with 75% ethyl alcohol with 2000mg/L hydrogen peroxide, with distillation after taking-up
Water carefully rinses at least 2 times；
B, it prepares with MS 0.3mg/L, niacin 0.3mg/L, folic acid 0.3mg/L, puridoxine hydrochloride 0.3mg/L, cobalamin
0.1mg/L, agar 2g/L, fructose 8g/L, creatinine 50mg/L, acid hydrolyzed casein 100mg/L, basic element of cell division 1mg/L are carried out
Aseptic seeding；
C, seed sprouts the protocorm for being initially formed green after 6 months, and protocorm is further differentiated to form the rhizomes of green,
There are multiple growing points on rhizomes, seedling out can be sprouted from the growing point of rhizomes；
D, can largely transfer proliferated culture medium on minimal medium, culture medium prescription: 1/2M basal medium, banana
Mud 20g/L, fructose 6g/L, active carbon 0.5g/L, grape juice 5g/L, corn juice 15g/L, yeast extract 30mg/L；
E, when seeing that blade-carrying a large amount of seedling occur in tissue culture bottle after 5-6 months, by seedling from 25 DEG C of constant humidity 35% of constant temperature
Environment move in 8000lux environment greenhouse, planted with fertile soil, apply exogenous nutrition substance, it is promoted to take root.
Embodiment 2
A, the roxburgh anoectochilus terminal bud wild fruit for harvesting not instant of complete cracking, with the thiocarbamide and chlorethanol of 150mg/L with the ratio of 1:1
Example mixed liquor cold soaking 3 days, and it is more than half an hour to mix disinfection with 75% ethyl alcohol with 2000mg/L hydrogen peroxide, with distillation after taking-up
Water carefully rinses at least 2 times；
B, it prepares with MS0.3mg/L, niacin 0.2mg/L, folic acid 0.2mg/L, puridoxine hydrochloride 0.3mg/L, cobalamin
0.3mg/L, agar 2g/L, fructose 8-11g/L, creatinine 30mg/L, acid hydrolyzed casein 100mg/L, basic element of cell division 1mg/L,
Carry out aseptic seeding；
C, seed sprouts the protocorm for being initially formed green after 6 months, and protocorm is further differentiated to form the rhizomes of green,
There are multiple growing points on rhizomes, seedling out can be sprouted from the growing point of rhizomes；
D, can largely transfer proliferated culture medium on minimal medium, culture medium prescription: 1/2M basal medium, banana
Mud 20g/L, fructose 10g/L, active carbon 0.1g/L, grape juice 5g/L, corn juice 15g/L, yeast extract 20mg/L；
E, when seeing that blade-carrying a large amount of seedling occur in tissue culture bottle after 5-6 months, by seedling from 25 DEG C of constant humidity 40% of constant temperature
Environment move in 7000lux environment greenhouse, planted with fertile soil, apply exogenous nutrition substance, it is promoted to take root.
Embodiment 3
A, the roxburgh anoectochilus terminal bud wild fruit for harvesting not instant of complete cracking, with the thiocarbamide and chlorethanol of 150mg/L with the ratio of 1:1
Example mixed liquor cold soaking 3 days, and it is more than half an hour to mix disinfection with 75% ethyl alcohol with 2000mg/L hydrogen peroxide, with distillation after taking-up
Water carefully rinses at least 2 times；
B, it prepares with MS0.3mg/L, niacin 0.3mg/L, folic acid 0.2mg/L, puridoxine hydrochloride 0.4mg/L, cobalamin
0.2mg/L, agar 2g/L, fructose 10g/L, creatinine 50mg/L, acid hydrolyzed casein 110mg/L, basic element of cell division 1mg/L, into
Row aseptic seeding；
C, seed sprouts the protocorm for being initially formed green after 6 months, and protocorm is further differentiated to form the rhizomes of green,
There are multiple growing points on rhizomes, seedling out can be sprouted from the growing point of rhizomes；
D, can largely transfer proliferated culture medium on minimal medium, culture medium prescription: 1/2M basal medium, banana
Mud 18g/L, fructose 8g/L, active carbon 0.3g/L, grape juice 8g/L, corn juice 12g/L, yeast extract 25mg/L；
E, when seeing that blade-carrying a large amount of seedling occur in tissue culture bottle after 5-6 months, by seedling from 25 DEG C of constant humidity 40% of constant temperature
Environment move in 8000lux environment greenhouse, planted with fertile soil, apply exogenous nutrition substance, it is promoted to take root.
In conclusion embodiment 2 is optimal case, compared to other the two, 2 seedling-growing time of embodiment is 6 months, children
Seedling survival rate is up to 92-93%, and 2% aberration rate, no obvious callus generation, therefore, embodiment 2 is optimal case.
The present invention is not limited to above-mentioned preferred forms, anyone obtain under the inspiration of the present invention other it is any with
The identical or similar product of the present invention, is within the scope of the present invention.
Claims (3)
1. wild gold lotus quick breeding propagation method, it is characterised in that: method includes the following steps:
A, the roxburgh anoectochilus terminal bud wild fruit for harvesting not instant of complete cracking is mixed with the thiocarbamide and chlorethanol of 150mg/L with the ratio of 1:1
Liquid cooling is soaked 3 days, and it is more than half an hour to mix disinfection with 75% ethyl alcohol with 2000mg/L hydrogen peroxide, careful with distilled water after taking-up
It rinses at least 2 times；
B, it prepares with MS 0.2-0.3mg/L, niacin 0.2-0.3mg/L, folic acid 0.2-0.3mg/L, puridoxine hydrochloride 0.3-
0.5mg/L, cobalamin 0.1-0.3mg/L, agar 1-2g/L, fructose 8-11g/L, creatinine 30-50mg/L, acid hydrolyzed casein
100-120mg/L, basic element of cell division 0.5-1mg/L carry out aseptic seeding；
C, seed sprouts the protocorm for being initially formed green after 6 months, and protocorm is further differentiated to form the rhizomes of green, root shape
There are multiple growing points on stem, seedling out can be sprouted from the growing point of rhizomes；
D, can largely transfer proliferated culture medium on minimal medium, culture medium prescription: 1/2M basal medium, banana puree
15-20g/L, fructose 5-10g/L, active carbon 0.1-0.5g/L, grape juice 5-8g/L, corn juice 10-15g/L, yeast extract
10-30mg/L；
E, when seeing that blade-carrying a large amount of seedling occur in tissue culture bottle after 5-6 months, by seedling from 24-27 DEG C of constant humidity 30- of constant temperature
45% environment moves in 6000lux-8000lux environment greenhouse, is planted with fertile soil, applies exogenous nutrition substance, promotes it
It takes root.
2. wild gold lotus quick breeding propagation method according to claim 1, it is characterised in that: in stepb, described thin
Born of the same parents' mitogen is that dihydro zeatin and 6- benzylaminopurine are mixed with 3:1 proportion.
3. wild gold lotus quick breeding propagation method according to claim 1, it is characterised in that: in step d, the Portugal
Grape juice is prepared as taking new fresh grape, removes its skin, and boiling 20 minutes in distilled water, then used filter mesh sieve filters its coarse stem
Item, the corn juice is placed in distilled water with fresh corn grain to be boiled with soft fire 30 minutes, is taken out particle and is squeezed, is squeezed out
Juice mixed with distilled water 2:1.
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| CN201811265662.6A CN109548651A (en) | 2018-10-29 | 2018-10-29 | Wild gold lotus quick breeding propagation method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111213586A (en) * | 2020-02-26 | 2020-06-02 | 四川农业大学 | Anoectochilus roxburghii tissue culture medium and preparation method thereof |
| WO2023029309A1 (en) * | 2021-09-01 | 2023-03-09 | 海南大学 | Ornamental dendrobium hybrid breeding method |
-
2018
- 2018-10-29 CN CN201811265662.6A patent/CN109548651A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111213586A (en) * | 2020-02-26 | 2020-06-02 | 四川农业大学 | Anoectochilus roxburghii tissue culture medium and preparation method thereof |
| WO2023029309A1 (en) * | 2021-09-01 | 2023-03-09 | 海南大学 | Ornamental dendrobium hybrid breeding method |
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