CN104082135A - Method for rapidly breeding dendrobium officinale seedlings - Google Patents
Method for rapidly breeding dendrobium officinale seedlings Download PDFInfo
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- CN104082135A CN104082135A CN201410257881.5A CN201410257881A CN104082135A CN 104082135 A CN104082135 A CN 104082135A CN 201410257881 A CN201410257881 A CN 201410257881A CN 104082135 A CN104082135 A CN 104082135A
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Abstract
The invention discloses a method for rapidly breeding dendrobium officinale seedlings, and belongs to the technical field of plant rapid breeding. The method comprises the following steps: (1) putting a dendrobium officinale protocorm into a protocorm proliferation culture medium for culturing; (2) transferring the protocorm into a cluster bud inductive agent proliferation culture medium for culturing; (3) cutting cluster buds, and putting the cut cluster buds into a rooting and strengthening culture medium for mass propagation; and (4) transplanting when seedlings grow to about 6-8 centimeters in heights. By adopting the method, the problems of virus carrying in seedlings, variety degeneration, low effective yield and the like in the current asexual reproduction of dendrobium officinale seedlings are solved, and a seedling culture method characterized by short period, high reproduction rate and low cost is provided for large-scale planting of dendrobium officinale under a manual control condition. Compared with the conventional breeding way, the method has the advantages that the yield is increased by about 80 percent, and the method has positive significance to the relief of market supply and demand contradiction of dendrobium officinale.
Description
Technical field
The invention belongs to plant fast propagation technical field, be specifically related to a kind of method of Fast-propagation dendrobium candidum seedling.
Background technology
Dendrobium candidum is one of most precious traditional Chinese medicine of China, is classified as " Chinese nine immortal grass " by honor.The application of dendrobium candidum, just on the books in Eastern Han Dynasty's Shennong's Herbal in the last years of a dynasty or reign, in the Ancient Times in China multi-section medical science works such as Compendium of Material Medica, " Chinese medicine dictionary ", all record the medicinal function of dendrobium candidum.Its ten main large effects of dendrobium candidum have: nourishing Tianjin: " Chinese pharmacy dictionary " title dendrobium candidum " gas-liquid of specially growing lung stomach, gas-liquid is rushed prosperous, kidney water is spontaneous ", be good at nourishing Yin and promoting production of body fluid, all diseases for the treatment of Yin and fluid deficiency.Build up health: dendrobium candidum has the function of nourishing yin and nourishing blood.Qing Dynasty's " property of medicine opinion " says: essence, nourishing stomach-YIN, beneficial strength are amassed in the kidney tonifying of dendrobium candidum energy.Contain abundant polysaccharose substance, there is the effect that strengthens immunologic function.Strengthen the spleen and stomach: dendrobium candidum is reinforcing stomach reg fluid medicine.All on the books in Shennong's Herbal, " book on Chinese herbal medicine is new again ", appellation " medicine for enterogastritis ", is the common drug that treatment epigastric pain, upper abdomen go up painful.Modern medicine experiment confirms: dendrobium candidum has good inhibitory action to pathogenic bacteria helicobacter pylori common in the spleen-stomach diseases, contributes to treat the illness of the Helicobacter pyloris such as atrophic gastritis, superficial gastritis, duodenal ulcer.Protect liver cholagogic: dendrobium candidum has good choleretic effect.Ancient Chinese medicine doctor all thinks that dendrobium candidum has the effect of nourishing hepatic yin, is the key medicine of the various liver and bladder diseases for the treatment of, can be used for treating the diseases in the liver and gallbladder such as hepatitis, cholecystitis, gall stone.Strong muscle lipopenicillinase: after people enters the middle age, muscles and bones function is faded, dendrobium candidum can nourishing liquid, lubricated joint, thereby reach strengthening the muscles and bones, fluent joint, strengthen antiheumatic effect.Modern pharmacological research also shows: dendrobium candidum can improve stress ability, has good antifatigue, resisting oxygen lack; Can promote circulation, hemangiectasis, reduction cholesterolemia and triglyceride.Reduce blood sugar: the replenishing the vital essence and removing heat of dendrobium candidum function is moisturized, and is exactly the medicine dedicated for the treatment of diabetes since ancient times.Clinical research shows, dendrobium candidum not only can strengthen insulin active, can significantly reduce blood sugar level simultaneously, makes blood sugar recovery normal.Suppress tumour: dendrobium candidum has killing action to some cell of the malignant tumours such as lung cancer, oophoroma and promyelocytic leukemia, has stronger antitumor activity.The clinical supplemental treatment for malignant tumour, can improve the symptom of tumor patient, and the side effect that alleviates Radiotherapy chemotherapy strengthens immunity, improves life quality, extends life span.Bright eyes: the stem of noble dendrobium has enriching yin and supports object function, the good merchantable brand by ancient Chinese medicine doctor as eye-protecting.Modern pharmacology studies confirm that: the stem of noble dendrobium is to preventing and treating senile cataract and protecting juvenile's eyesight to have positive effect.Nourish skin: human body entered after the middle age, because the cloudy liquid in body reduces day by day, thereby had accelerated skin aging, made it blackening or crumple, and the lymphatic temperament that dendrobium candidum contains, has moist trophism to human body skin.Promote longevity: Shennong's Herbal is classified dendrobium candidum as the panacea with " make light of one's life by commiting suicide and prolong life " effect.Modern pharmacology studies confirm that: dendrobium candidum contains various trace elements, has close relationship for the good health and a long life of human body, to the anti-aging effects of human body than general medicine more extensively, more comprehensively.Because wild dendrobium candidum requires extremely harshness to natural ecological condition, natural propagation rate is extremely low again, and as far back as eighties of last century the eighties, dendrobium candidum is just classified as the Endangered Medicinal Herb of treasuring of focused protection by country.Because dendrobium candidum is grown nonparasitically upon another plant on large trunk or rock more.Can not grow in common soil, bloom many, result is few, every fruit approximately has 1,000,000, seed, plants carefully as dust, and the growth of embryo is no more than the spherical stage, lack again endosperm tissue, must descend slowly and lightly and could sprout to unusual adapt circumstance, the germination rate of natural conditions is not as good as 5%, and growing very slowly, is only originally a tiny green pearl, and photosynthesis area is very little, intensity is low, very strict to habitat conditions requirement, therefore its sexual propagation difficulty, reproduction coefficient is low, and growth is slow.The present invention adopts unique cultivation formula, has solved seedling that current dendrobium candidum seedling vegetative propagation exists and has carried the problems such as virus, deterioration of variety, effective output are low.Under manual control condition, for the implant mass of dendrobium candidum provides the seedling-cultivating method that a kind of cycle is short, reproduction rate is high, with low cost, compared with traditional modes of reproduction, output improves approximately 80%, and the demand and supply contraction of alleviating dendrobium candidum is had to positive effect.
Summary of the invention
The problem that the present invention solves is to provide a kind of method of Fast-propagation dendrobium candidum seedling, and this method is the virus-free seedling-cultivating method carrying that a kind of cycle is short, reproduction rate is high, with low cost.
The present invention is achieved through the following technical solutions:
The method of Fast-propagation dendrobium candidum seedling, comprises the following steps:
1) dendrobium candidum protocorm is positioned in Protocorm Multiplication medium and is cultivated: by being forwarded to Protocorm Multiplication medium after protocorm cutting, to obtain a large amount of protocorms;
2) protocorm is forwarded in inducing clumping bud agent proliferated culture medium and cultivates: protocorm is forwarded to inducing clumping bud and proliferated culture medium, within 15～20 days, breed a large amount of Multiple Buds, cut as required Multiple Buds, be forwarded to fresh adventitious buds proliferation medium, about 10 days, can gather in the crops the Multiple Buds of propagation, within 20～30 days, Multiple Buds can be multiplied into the high approximately seedling of 2～3cm;
3) cutting Multiple Buds is positioned over and in Rooting and hardening-off culture base, makes its amount reproduction: seedling is forwarded to Rooting and hardening-off culture base, after approximately 30 days, can obtain high 6～8cm, well developed root system, dendrobium candidum seedling that rhizome is sturdy, the hardening of uncapping under indoor natural light 3～5 days, uses in order to transplanting;
4) in the time that growing up to high approximately 6～8cm, seedling transplants.
Described step 1) in being configured to of protocorm proliferated culture medium: 0.8mg/LNAA, 0.2mg/L6_BA and 100g/L banana puree, at 100 DEG C of sterilizing 30min.
Described step 2) in the MS solid culture medium that is configured to of inducing clumping bud agent proliferated culture medium add 0.8～1.0mg/Kg6-benayl aminopurine, 25～35g/Kg sucrose and 4～6g/Kg agar; Condition of culture is: 23～25 DEG C of temperature, relative moisture 75～90%, light application time 10～12 hours/day, intensity of illumination 3000～5000Lx.
Described step 3) in the 1/2MS solid culture medium that is configured to of Rooting and hardening-off culture base add 0.2～0.5mg/Kg methyl α-naphthyl acetate, 50～60g/Kg murphy juice, 165～200g/Kg tomato juice, 8～12g/Kg active carbon, 10～25g/Kg sucrose and 4～6g/Kg agar; Condition of culture is: 23～25 DEG C of temperature, relative moisture 75～90%, light application time 10～12 hours/day, intensity of illumination 3000～5000Lx.
Described step 4) in dendrobium candidum seedling is carried out to the tpn immersion bubble dendrobium candidum root with 800～1000 times of dilutions before acclimatization and transplants.
Compared with prior art, the present invention has following useful technique effect:
The method of a kind of Fast-propagation dendrobium candidum of the present invention seedling, has solved seedling that current dendrobium candidum seedling vegetative propagation exists and has carried the problems such as virus, deterioration of variety, effective output are low.Under manual control condition, for the implant mass of dendrobium candidum provides the seedling-cultivating method that a kind of cycle is short, reproduction rate is high, with low cost, compared with traditional modes of reproduction, output improves approximately 80%, and the demand and supply contraction of alleviating dendrobium candidum is had to positive effect.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail, and the explanation of the invention is not limited.
Embodiment 1
A method for Fast-propagation dendrobium candidum seedling, comprises the following steps:
1) dendrobium candidum protocorm is positioned in Protocorm Multiplication medium and is cultivated: by 0.8mg/LNAA, 0.2mg/L6_BA and 100g/L banana puree, dendrobium candidum protocorm is placed in to medium cultivation after 100 DEG C of sterilizing 30min and has obtained a large amount of dendrobium candidum protocorms.
2) protocorm is forwarded in inducing clumping bud agent proliferated culture medium and cultivates: add 0.8mg/Kg6-benayl aminopurine, 25/Kg sucrose and 4g/Kg agar at MS solid culture medium; Condition of culture is: 23 DEG C of temperature, relative moisture 75%, light application time 10 hours/day, intensity of illumination 3000Lx.
3) cutting Multiple Buds is positioned over and in Rooting and hardening-off culture base, makes its amount reproduction: seedling is forwarded in the Rooting and hardening-off culture base that 1/2MS solid culture medium adds 0.2mg/Kg methyl α-naphthyl acetate, 50/Kg murphy juice, 165/Kg tomato juice, 8g/Kg active carbon, 10/Kg sucrose and 4g/Kg agar and is cultivated, condition of culture is: 23 DEG C of temperature, relative moisture 75%, light application time 10 hours/day, intensity of illumination 3000Lx, after approximately 30 days, can obtain high 6cm, well developed root system, dendrobium candidum seedling that rhizome is sturdy, the hardening of uncapping under indoor natural light 3 days, uses in order to transplanting.
4) in the time that growing up to high about 6cm, seedling transplants after the tpn immersion bubble dendrobium candidum root with 800 times of dilutions.
Embodiment 2
A method for Fast-propagation dendrobium candidum seedling, comprises the following steps:
1) dendrobium candidum protocorm is positioned in Protocorm Multiplication medium and is cultivated: by 0.8mg/LNAA, 0.2mg/L6_BA and 100g/L banana puree, dendrobium candidum protocorm is placed in to medium cultivation after 100 DEG C of sterilizing 30min and has obtained a large amount of dendrobium candidum protocorms.
2) protocorm is forwarded in inducing clumping bud agent proliferated culture medium and cultivates: add 0.9mg/Kg6-benayl aminopurine, 28/Kg sucrose and 5g/Kg agar at MS solid culture medium; Condition of culture is: 25 DEG C of temperature, relative moisture 85%, light application time 11 hours/day, intensity of illumination 4000Lx.
3) cutting Multiple Buds is positioned over and in Rooting and hardening-off culture base, makes its amount reproduction: seedling is forwarded in the Rooting and hardening-off culture base that 1/2MS solid culture medium adds 0.3mg/Kg methyl α-naphthyl acetate, 50/Kg murphy juice, 185/Kg tomato juice, 8/Kg active carbon, 15/Kg sucrose and 5g/Kg agar and is cultivated, condition of culture is: 25 DEG C of temperature, relative moisture 90%, light application time 11 hours/day, intensity of illumination 4000Lx, after approximately 30 days, can obtain high 6cm, well developed root system, dendrobium candidum seedling that rhizome is sturdy, the hardening of uncapping under indoor natural light 3 days, uses in order to transplanting.
4) in the time that growing up to high about 6cm, seedling transplants after the tpn immersion bubble dendrobium candidum root with 1000 times of dilutions.
Embodiment 3
A method for Fast-propagation dendrobium candidum seedling, comprises the following steps:
1) dendrobium candidum protocorm is positioned in Protocorm Multiplication medium and is cultivated: by 0.8mg/LNAA, 0.2mg/L6_BA and 100g/L banana puree, dendrobium candidum protocorm is placed in to medium cultivation after 100 DEG C of sterilizing 30min and has obtained a large amount of dendrobium candidum protocorms.
2) protocorm is forwarded in inducing clumping bud agent proliferated culture medium and cultivates: add 1mg/Kg6-benayl aminopurine, 30/Kg sucrose and 6g/Kg agar at MS solid culture medium; Condition of culture is: 23 DEG C of temperature, relative moisture 85%, light application time 11 hours/day, intensity of illumination 3000Lx.
3) cutting Multiple Buds is positioned over and in Rooting and hardening-off culture base, makes its amount reproduction: seedling is forwarded in the Rooting and hardening-off culture base that 1/2MS solid culture medium adds 0.3mg/Kg methyl α-naphthyl acetate, 60/Kg murphy juice, 180/Kg tomato juice, 8/Kg active carbon, 12/Kg sucrose and 4g/Kg agar and is cultivated, condition of culture is: 23 DEG C of temperature, relative moisture 85%, light application time 12 hours/day, intensity of illumination 3000Lx, after approximately 30 days, can obtain high 6cm, well developed root system, dendrobium candidum seedling that rhizome is sturdy, the hardening of uncapping under indoor natural light 3 days, uses in order to transplanting.
4) in the time that growing up to high about 6cm, seedling transplants after the tpn immersion bubble dendrobium candidum root with 800 times of dilutions.
Embodiment 4
A method for Fast-propagation dendrobium candidum seedling, comprises the following steps:
1) dendrobium candidum protocorm is positioned in Protocorm Multiplication medium and is cultivated: by 0.8mg/LNAA, 0.2mg/L6_BA and 100g/L banana puree, dendrobium candidum protocorm is placed in to medium cultivation after 100 DEG C of sterilizing 30min and has obtained a large amount of dendrobium candidum protocorms.
2) protocorm is forwarded in inducing clumping bud agent proliferated culture medium and cultivates: add 0.8mg/Kg6-benayl aminopurine, 30/Kg sucrose and 6g/Kg agar at MS solid culture medium; Condition of culture is: 23 DEG C of temperature, relative moisture 90%, light application time 12 hours/day, intensity of illumination 5000Lx.
3) cutting Multiple Buds is positioned over and in Rooting and hardening-off culture base, makes its amount reproduction: seedling is forwarded in the Rooting and hardening-off culture base that 1/2MS solid culture medium adds 0.2mg/Kg methyl α-naphthyl acetate, 60/Kg murphy juice, 200/Kg tomato juice, 8/Kg active carbon, 15/Kg sucrose and 4g/Kg agar and is cultivated, condition of culture is: 25 DEG C of temperature, relative moisture 90%, light application time 12 hours/day, intensity of illumination 5000Lx, after approximately 30 days, can obtain high 8cm, well developed root system, dendrobium candidum seedling that rhizome is sturdy, the hardening of uncapping under indoor natural light 3 days, uses in order to transplanting.
4) in the time that growing up to high about 8cm, seedling transplants after the tpn immersion bubble dendrobium candidum root with 1000 times of dilutions.
Embodiment 5
A method for Fast-propagation dendrobium candidum seedling, comprises the following steps:
1) dendrobium candidum protocorm is positioned in Protocorm Multiplication medium and is cultivated: by 0.8mg/LNAA, 0.2mg/L6_BA and 100g/L banana puree, dendrobium candidum protocorm is placed in to medium cultivation after 100 DEG C of sterilizing 30min and has obtained a large amount of dendrobium candidum protocorms.
2) protocorm is forwarded in inducing clumping bud agent proliferated culture medium and cultivates: add 0.8mg/Kg6-benayl aminopurine, 25/Kg sucrose and 6g/Kg agar at MS solid culture medium; Condition of culture is: 25 DEG C of temperature, relative moisture 75%, light application time 12 hours/day, intensity of illumination 5000Lx.
3) cutting Multiple Buds is positioned over and in Rooting and hardening-off culture base, makes its amount reproduction: seedling is forwarded in the Rooting and hardening-off culture base that 1/2MS solid culture medium adds 0.5mg/Kg methyl α-naphthyl acetate, 60/Kg murphy juice, 200/Kg tomato juice, 8/Kg active carbon, 10/Kg sucrose and 6g/Kg agar and is cultivated, condition of culture is: 25 DEG C of temperature, relative moisture 85%, light application time 10 hours/day, intensity of illumination 5000Lx, after approximately 30 days, can obtain high 8cm, well developed root system, dendrobium candidum seedling that rhizome is sturdy, the hardening of uncapping under indoor natural light 3 days, uses in order to transplanting.
4) in the time that growing up to high about 8cm, seedling transplants after the tpn immersion bubble dendrobium candidum root with 1000 times of dilutions.
Claims (5)
1. a method for Fast-propagation dendrobium candidum seedling, is characterized in that, comprises the following steps:
1) dendrobium candidum protocorm is positioned in Protocorm Multiplication medium and is cultivated.
2) protocorm is forwarded in inducing clumping bud agent proliferated culture medium and cultivates.
3) cutting Multiple Buds is positioned over and in Rooting and hardening-off culture base, makes its amount reproduction.
4) in the time that growing up to high approximately 6～8cm, seedling transplants.
2. the method for a kind of Fast-propagation dendrobium candidum seedling according to claim 1, it is characterized in that: described step 1) in being configured to of protocorm proliferated culture medium: 0.8mg/LNAA, 0.2mg/L6_BA and 100g/L banana puree, at 100 DEG C of sterilizing 30min.
3. the method for a kind of Fast-propagation dendrobium candidum seedling according to claim 1, is characterized in that: described step 2) described in being configured to of inducing clumping bud agent proliferated culture medium: MS solid culture medium adds 0.8～1.0mg/Kg6-benayl aminopurine, 25～35g/Kg sucrose and 4～6g/Kg agar; Condition of culture is: 23～25 DEG C of temperature, relative moisture 75～90%, light application time 10～12 hours/day, intensity of illumination 3000～5000Lx.
4. the method for a kind of Fast-propagation dendrobium candidum seedling according to claim 1, is characterized in that: described step 3) described in being configured to of Rooting and hardening-off culture base: 1/2MS solid culture medium adds 0.2～0.5mg/Kg methyl α-naphthyl acetate, 50～60g/Kg murphy juice, 165～200g/Kg tomato juice, 8～12g/Kg active carbon, 10～25g/Kg sucrose and 4～6g/Kg agar; Condition of culture is: 23～25 DEG C of temperature, relative moisture 75～90%, light application time 10～12 hours/day, intensity of illumination 3000～5000Lx.
5. the method for a kind of Fast-propagation dendrobium candidum seedling according to claim 1, is characterized in that: described step 4) in dendrobium candidum seedling is carried out to the tpn immersion bubble dendrobium candidum root with 800～1000 times of dilutions before acclimatization and transplants.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104604688A (en) * | 2015-02-05 | 2015-05-13 | 东莞广州中医药大学中医药数理工程研究院 | In-vitro culture method capable of promoting efficient propagation of dendrobium officinale by nano materials having antibacterial effect |
| CN108739317A (en) * | 2018-05-20 | 2018-11-06 | 济宁盛知华知识产权服务有限公司 | A kind of inoculation method of dendrobium candidum |
| CN117084173A (en) * | 2023-09-19 | 2023-11-21 | 中国热带农业科学院热带作物品种资源研究所 | Method for culturing dendrobium candidum tissue culture seedlings by using tissue culture bags |
-
2014
- 2014-06-11 CN CN201410257881.5A patent/CN104082135A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104604688A (en) * | 2015-02-05 | 2015-05-13 | 东莞广州中医药大学中医药数理工程研究院 | In-vitro culture method capable of promoting efficient propagation of dendrobium officinale by nano materials having antibacterial effect |
| CN108739317A (en) * | 2018-05-20 | 2018-11-06 | 济宁盛知华知识产权服务有限公司 | A kind of inoculation method of dendrobium candidum |
| CN117084173A (en) * | 2023-09-19 | 2023-11-21 | 中国热带农业科学院热带作物品种资源研究所 | Method for culturing dendrobium candidum tissue culture seedlings by using tissue culture bags |
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