CN109541249A - A kind of light-operated micro-fluidic high-volume sampling device - Google Patents
A kind of light-operated micro-fluidic high-volume sampling device Download PDFInfo
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- CN109541249A CN109541249A CN201811336490.7A CN201811336490A CN109541249A CN 109541249 A CN109541249 A CN 109541249A CN 201811336490 A CN201811336490 A CN 201811336490A CN 109541249 A CN109541249 A CN 109541249A
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- China
- Prior art keywords
- light
- micro
- operated
- fluidic
- fluidic chip
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1065—Multiple transfer devices
Abstract
The present invention relates to a kind of light-operated micro-fluidic high-volume sampling devices.Light-operated micro-fluidic high-volume sampling device includes automatic sampling apparatus, light-operated mobile device, micro-fluidic chip, mass spectrometer interface.Automatic sampling apparatus, sample to be tested is automatically added to micro-fluidic chip entrance, micro-fluidic chip channel is under illumination effect, corresponding deformation occurs with intensity of illumination difference, sample drop is moved because of the extruding force that channel deformation generates, it is used to control sample by light-operated mobile device and is moved in micro-fluidic chip, to realizes light-operated loading.Mass spectrometer interface is connect in micro-fluidic chip exit, makes sample after ionized, is detected into mass spectrum.The present invention realizes trace complex biological sample high-volume, automatical analysis, improves detection efficiency in the way of light-operated loading.
Description
Technical field
The present invention relates to analysis and detecting instrument fields, more particularly to a kind of light-operated micro-fluidic high-volume sampling device.
Background technique
Microfluidic chip technology is by bases such as sample treatment, reaction, analysis detections during life science, clinical detection
This operating process is integrated on the chip of micron order scale, to realize that miniature full-automatic is analyzed.Micro-fluidic chip is small by its
Ingeniously, efficiently the features such as, plays increasingly important role in fields such as cell analysis, trace detection, biologic medicals.
Important technology of the biological mass spectrometry as modern analysis is capable of providing the qualitative, quantitative information of sample to be tested, and detects
High sensitivity, accuracy are good, and the type for being able to detect biomolecule is more, and mass range is big.Therefore micro-fluidic chip and mass spectrum join
With can guarantee quick, high-throughput accurately to analyze biological sample.
In existing micro-fluidic chip and mass spectrometric hyphenated technique, the mode that sample drives frequently with pump in micro-fluidic chip,
It is fixedly connected with pipe at the both ends of chip, then connecting tube is connected with peristaltic pump or syringe pump, the liquid in chip is made to exist
It is subjected to displacement under pumping action.Many and diverse, single unit system heaviness is connected between such driving method pipe, it is not light enough.And in reality
Though conventional sample can be more sensitively detected in, in clinical detection, because biological specimen kind is various, complicated component,
Therefore analysis detection personnel are caused with very big challenge.Detection for trace materials, simple mass spectral analysis will cause sample
Waste, it is not comprehensive enough accurate to cause to analyze result, thus designs a kind of device for high-volume trace species analysis and seem outstanding
It is important.
Summary of the invention
In order to solve the above problem, the purpose of the present invention is to provide a kind of light-operated micro-fluidic high-volume sampling devices, by light
Micro-fluidic and mass spectrum coupling apparatus is controlled, using the effect of illumination, sample is driven to move in chip channel.Light-operated advantage is
Complicated cumbersome channel connection is not needed, can in the non-contact case, real-time, accurate, lossless control liquid is in channel
In movement, be conducive to the simplification of micro-fluidic sampling device.Under the action of light-operated, the sample of chip end is moved to, in matter
It composes and realizes sample ionization at ion source interface, subsequently into the progress subsequent detection analysis into mass spectrum.The device can be realized
The automation loading of high-volume biological sample, and can guarantee the accurate detection of trace materials.
The light-operated micro-fluidic high-volume sampling device of one kind proposed by the present invention, including automatic sampling apparatus, light-operated mobile dress
Set, micro-fluidic chip and mass spectrometer interface, be evenly arranged several channels in micro-fluidic chip, light-operated mobile device by light source,
Movable supporting frame and motor form, light source are set on movable supporting frame, and light source is mobile by movable supporting frame, described removable
Dynamic bracket connects motor, and motor is used to control the movement of movable supporting frame；The number of channels of the micro-fluidic chip and light source institute
The quantity of lamp containing light is consistent；The feeder connection of the outlet alignment micro-fluidic chip of automatic sampling apparatus, the micro-fluidic chip
Channel exit be equipped with mass spectrometer interface；Sample to be tested is automatically added to the logical of micro-fluidic chip by the automatic sampling apparatus
Road entrance；Light-operated mobile device is used to control sample to be moved in micro-fluidic chip, and light-operated movement routine can be according to micro-fluidic logical
The motion path of road shape, i.e. sample to be tested is determined；Micro-fluidic chip channel is under illumination effect, with intensity of illumination difference
And corresponding deformation occurs, sample drop is moved because of the extruding force that channel deformation generates, to realize light-operated loading；Micro-fluidic
Chip exit connects mass spectrometer interface, makes sample after ionized, is detected into mass spectrum.
In the present invention, the light source is by the light lamp group of multiple parallel arrangeds at the switch of each smooth lamp can independent control.
In the present invention, the light in the light-operated mobile device can be the light of any wavelength such as visible light, infrared, ultraviolet.
In the present invention, the motor in the light-operated mobile device can control device shifting for stepper motor, servo motor etc.
It is dynamic.
In the present invention, described micro-fluidic chip upper channel quantity 1 ~ 500.
In the present invention, the microchannel quantity and the light lamp quantity of composition light source are consistent.
In the present invention, the micro-fluidic chip channel can be linear type, can also use flexure type to increase passage length.
In the present invention, the light-operated movement routine should be consistent with channel shape, that is, sample drop motion path.
In the present invention, the mass spectrometer interface can be convenient for combining simultaneously with micro-fluidic chip for ESI interface, MADLI interface etc.
It can be by the mass spectrometer interface of sample ionization.
In the present invention, when the mass spectrometer interface selects ESI interface, it should be sprayed in micro-fluidic chip channel exit and capillary
Needle coupling is detected so that sample is ionized under the action of electric field into ESI-MS.
Compared with prior art, the device have the advantages that are as follows:
1) by illumination, in the non-contact case, real-time, accurate, lossless control liquid is mobile, simplifies micro fluidic device.
2) it realizes the automatic loading of high-volume, simplifies operation, improve detection efficiency.
3) trace materials is sequentially introduced into mass spectrum and is detected, reduced sample waste, be advantageously implemented accurate analysis.
Detailed description of the invention
Fig. 1 is the light-operated micro-fluidic high-volume sampling device block diagram of the embodiment of the present invention；
Fig. 2 is the light-operated micro-fluidic high-volume sampling device overall schematic of the embodiment of the present invention；
Fig. 3 is the light-operated mobile device schematic diagram of the embodiment of the present invention；
Figure label: 1 is automatic sampling apparatus, and 22 be light-operated mobile device, and 3 be micro-fluidic chip, and 4 be mass spectrometer interface, and 5 be light
Lamp, 6-1,6-2,6-3 are respectively first passage, second channel and third channel, and 7 be movable supporting frame, and 8 be capillary nozzle needle.
Specific embodiment
Explanation is described in detail embodiment of the invention and instructs how those skilled in the art realize this below
The reproduction of invention.In order to instruct technical solution of the present invention, some conventional aspects for having simplified or having omitted.Those skilled in the art
It should be understood that from these embodiments deformation or will within the scope of the invention.Those skilled in the art should understand that following
Feature can be combined in various ways to form multiple deformations of the invention.The invention is not limited to the optional realities of subordinate as a result,
Mode is applied, and only claim and their equivalent limits.
Embodiment 1: in conjunction with Fig. 1, Fig. 2, Fig. 3, light-operated micro-fluidic high-volume sampling device includes automatic sampling apparatus 1, light
Control mobile device 2, micro-fluidic chip 3, mass spectrometer interface 4.Sample in sample cell is introduced micro-fluidic chip by automatic sampling apparatus 1
Each feeder connection selects the number of channels of loading according to actual experiment demand.Light source in light-operated mobile device 2 is by a series of
The light lamp 5 of parallel arranged forms, and light lamp quantity is consistent with micro-fluidic chip upper channel quantity.The switch of each smooth lamp can be only
Vertical control, to realize that the sample drop in every channel can be controlled separately.Light-operated mobile device 2 is by light source, movable supporting frame
And motor form, in which: movable supporting frame is made of pedestal, support rod and lamp holder, and strip slot, support rod one are provided on pedestal
End is located in strip slot, and can move back and forth in the slot, and the support rod other end connects lamp holder one end, and lamp holder is equipped with several
Light lamp 5.In the present embodiment, first passage 6-1, second channel 6-2, third channel 6-3 entrance loading has sample to be tested, by light
Corresponding smooth lamp is lighted in source, is slowly moved from motor control movable supporting frame 7 to chip exit.In this process
In, the movement of light source can generate light-intensity difference in the part range of chip channel, and causing channel, deformation occurs, squeeze sample
The movement of drop chip exit stops when whole samples drop moves under the action of light-operated exports 1 ~ 10mm away from chip
Only motor movement, while only retaining smooth lamp corresponding with channel 6-1, remaining extinguishing.Start motor, light lamp only control channel 6-
Sample drop in 1 moves forward, and enters in the capillary nozzle needle 8 of micro-fluidic chip end, under the action of voltage,
Electron spray is formed, ESI-MS analysis is carried out.After the completion of sample drop analysis, other samples before being returned by motor control light source
Smooth lamp corresponding with second channel 6-2 is only lighted in stop place, continues aforementioned operation, should ensure that ESI mass spectrum connects at this time
Mouthful, it is corresponding with the 2nd 6-2 channel end capillary nozzle needle 8.The loading of rest channels, it is similar.
In the present embodiment, it is preferable that select linear type channel.In other embodiments, if the sample of required analysis
Product amount is big, and flexure type chip channel can be selected, and extending channel length saves loading time early period, improves analysis rate.
In the present embodiment, it is preferable that micro-fluidic chip is coupled with capillary nozzle needle, carries out ESI-MS analysis.At it
In his embodiment, MALDI-MS also can be selected, it is customized according to analysis demand.
In the present embodiment, it is preferable that light-operated movement routine should be consistent with channel shape, that is, sample drop motion path.
It should also be consistent in other embodiments.
In the present embodiment, it is preferable that the light source of used light lamp is ultraviolet light.In other embodiments, it is seen that
The light source of any wavelength such as light, infrared, ultraviolet.
The above-described embodiments merely illustrate the principles and effects of the present invention, and is not intended to limit the present invention.It is any ripe
The personage for knowing this technology all without departing from the spirit and scope of the present invention, carries out modifications and changes to above-described embodiment.Cause
This, institute is complete without departing from the spirit and technical ideas disclosed in the present invention by those of ordinary skill in the art such as
At all equivalent modifications or change, should be covered by the claims of the present invention.
Claims (10)
1. a kind of light-operated micro-fluidic high-volume sampling device, it is characterised in that: including automatic sampling apparatus, light-operated mobile device,
Micro-fluidic chip and mass spectrometer interface, have been evenly arranged several channels in micro-fluidic chip, and light-operated mobile device is by light source, removable
Dynamic bracket and motor form, light source are set on movable supporting frame, and light source is mobile by movable supporting frame, the removable branch
Frame connects motor, and motor is used to control the movement of movable supporting frame；Light contained by the number of channels and light source of the micro-fluidic chip
Lamp quantity is consistent；The feeder connection of the outlet alignment micro-fluidic chip of automatic sampling apparatus, the micro-fluidic chip lead to
Road exit is equipped with mass spectrometer interface；The automatic sampling apparatus enters the channel that sample to be tested is automatically added to micro-fluidic chip
Mouthful；Light-operated mobile device is used to control sample to be moved in micro-fluidic chip, and light-operated movement routine can be according to microfluidic channel shape
The motion path of shape, i.e. sample to be tested is determined；It is sent out under illumination effect with intensity of illumination difference in micro-fluidic chip channel
Raw corresponding deformation, sample drop is moved because of the extruding force that channel deformation generates, to realize light-operated loading；In micro-fluidic chip
Exit connects mass spectrometer interface, makes sample after ionized, is detected into mass spectrum.
2. light-operated micro-fluidic high-volume sampling device according to claim 1, it is characterised in that: the automatic sampling apparatus
It may include multiple sample injection bottles for storing multiple samples, to be advantageously implemented high-volume loading.
3. light-operated micro-fluidic high-volume sampling device according to claim 1, it is characterised in that: the light source by arranging parallel
The light lamp group of column is at the switch of each smooth lamp can independent control.
4. light-operated micro-fluidic high-volume sampling device according to claim 1, it is characterised in that: the light-operated mobile device
In light be visible light, infrared light or ultraviolet light in it is any.
5. light-operated micro-fluidic high-volume sampling device according to claim 1, it is characterised in that: the light-operated mobile device
In motor be stepper motor or servo motor.
6. light-operated micro-fluidic high-volume sampling device according to claim 1, it is characterised in that: on the micro-fluidic chip
Number of channels be 1 ~ 500.
7. light-operated micro-fluidic high-volume sampling device according to claim 1, it is characterised in that: the micro-fluidic chip is logical
Road is linear type, or uses flexure type to increase passage length.
8. light-operated micro-fluidic high-volume sampling device according to claim 1, it is characterised in that: the light-operated movement routine
It should be consistent with channel shape, that is, sample drop motion path.
9. light-operated micro-fluidic high-volume sampling device according to claim 1, it is characterised in that: the mass spectrometer interface is
ESI interface or MADLI interface.
10. light-operated micro-fluidic high-volume sampling device according to claim 1, it is characterised in that: the mass spectrometer interface choosing
It when with ESI interface, should be coupled in micro-fluidic chip channel exit with capillary nozzle needle, so that sample makes under the action of electric field
Sample ionization is detected into ESI-MS.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811336490.7A CN109541249A (en) | 2018-12-20 | 2018-12-20 | A kind of light-operated micro-fluidic high-volume sampling device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811336490.7A CN109541249A (en) | 2018-12-20 | 2018-12-20 | A kind of light-operated micro-fluidic high-volume sampling device |
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| CN109541249A true CN109541249A (en) | 2019-03-29 |
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| CN201811336490.7A Pending CN109541249A (en) | 2018-12-20 | 2018-12-20 | A kind of light-operated micro-fluidic high-volume sampling device |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090078326A1 (en) * | 2004-06-14 | 2009-03-26 | Arizona Board Of Regents For And On Behalf Of Arizona State University | Light-driven microfluidic devices and amplification of stimulus-induced wetting |
| CN102466655A (en) * | 2010-11-16 | 2012-05-23 | 上海华质生物技术有限公司 | Detection device and method of combined use of micro-fluidic chip and mass spectrometry |
| CN103226127A (en) * | 2013-03-27 | 2013-07-31 | 清华大学 | Multi-channel micro-fluidic chip and mass spectrum combined device |
| CN107029565A (en) * | 2017-05-25 | 2017-08-11 | 合肥工业大学 | Optical drive double-layered compound film based on graphene oxide and preparation method and application |
| CN107676541A (en) * | 2016-08-02 | 2018-02-09 | 复旦大学 | A kind of method of light-operated micro-fluid movement |
| CN108514901A (en) * | 2018-06-01 | 2018-09-11 | 东莞东阳光科研发有限公司 | A kind of digital microcurrent-controlled chip |
| CN108620143A (en) * | 2018-06-28 | 2018-10-09 | 京东方科技集团股份有限公司 | Digital microcurrent-controlled chip and its driving method |
-
2018
- 2018-12-20 CN CN201811336490.7A patent/CN109541249A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090078326A1 (en) * | 2004-06-14 | 2009-03-26 | Arizona Board Of Regents For And On Behalf Of Arizona State University | Light-driven microfluidic devices and amplification of stimulus-induced wetting |
| CN102466655A (en) * | 2010-11-16 | 2012-05-23 | 上海华质生物技术有限公司 | Detection device and method of combined use of micro-fluidic chip and mass spectrometry |
| CN103226127A (en) * | 2013-03-27 | 2013-07-31 | 清华大学 | Multi-channel micro-fluidic chip and mass spectrum combined device |
| CN107676541A (en) * | 2016-08-02 | 2018-02-09 | 复旦大学 | A kind of method of light-operated micro-fluid movement |
| CN107029565A (en) * | 2017-05-25 | 2017-08-11 | 合肥工业大学 | Optical drive double-layered compound film based on graphene oxide and preparation method and application |
| CN108514901A (en) * | 2018-06-01 | 2018-09-11 | 东莞东阳光科研发有限公司 | A kind of digital microcurrent-controlled chip |
| CN108620143A (en) * | 2018-06-28 | 2018-10-09 | 京东方科技集团股份有限公司 | Digital microcurrent-controlled chip and its driving method |
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Application publication date: 20190329 |