CN109536373A - A kind of Automatic-exhausting pickle reagent reaction tube - Google Patents
A kind of Automatic-exhausting pickle reagent reaction tube Download PDFInfo
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- CN109536373A CN109536373A CN201811424083.1A CN201811424083A CN109536373A CN 109536373 A CN109536373 A CN 109536373A CN 201811424083 A CN201811424083 A CN 201811424083A CN 109536373 A CN109536373 A CN 109536373A
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- reagent
- chamber
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- Life Sciences & Earth Sciences (AREA)
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- Sustainable Development (AREA)
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- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of Automatic-exhausting pickle reagent reaction tubes, including the preset room of liquid inlet conduit, reagent, exhaust chamber, reaction chamber, exhaust passage and outtake tube；The liquid inlet conduit is sequentially connected the preset room of reagent, exhaust chamber, reaction chamber by intake chute；The exhaust chamber connects outtake tube by exhaust passage；The air guide column array that the bottom of the exhaust chamber has spacing to be less than 1mm, air guide column have certain tilt angle according to liquid flow direction, and top is domed, and top opening connects exhaust passage, and opening pastes waterproof ventilated membrane.Bubble in PCR reagent can be carried out gas-liquid separation by exhaust chamber by apparatus of the present invention, guarantee that reagent can fill full reaction chamber, which has the characteristics that compact-sized, small in size, gas-liquid separation effect is good.
Description
Technical field
The present invention relates to a kind of design of reagent reaction tube more particularly to a kind of Automatic-exhausting pickle reagent reaction tubes.
Background technique
The research of nucleic acid purification and real-time fluorescence PCR reaction integrated instrument is applied due to its high degree of automation, reaction
Process is pollution-free, and oneself is increasingly becomed the main direction of development of real-time fluorescence PCR instrument, but by the sample of nucleic acid of purification from turn
A large amount of bubbles are had when moving on in PCR reagent pipe to generate, and are especially being transported with the PCR reaction tube for reacting preset reagent solution
Since vibration generates a large amount of non-uniform bubbles in journey, bubble will directly affect experimental result in PCR reaction process, how
The a great problem that bubble is the design of PCR reaction tube is discharged in the case where not increasing experimental arrangement rapidly, constrains this technology
Using and promote.
Summary of the invention
Goal of the invention: in view of the above problems, the present invention proposes a kind of Automatic-exhausting pickle reagent reaction tube.
Technical solution: to achieve the purpose of the present invention, the technical scheme adopted by the invention is that: a kind of Automatic-exhausting pickle examination
Agent reaction tube, including the preset room of liquid inlet conduit, reagent, exhaust chamber, reaction chamber, exhaust passage and outtake tube；The feed liquor is led
Pipe is sequentially connected the preset room of reagent, exhaust chamber, reaction chamber by intake chute；The exhaust chamber connects out conductance by exhaust passage
Pipe.
Further, air guide column is arranged at the bottom of the exhaust chamber, and top is domed, and top opening connects exhaust passage, opens
Waterproof ventilated membrane is pasted at mouthful.
Further, the air guide column array that the bottom of the exhaust chamber has spacing to be less than 1mm, air guide column are flowed according to liquid
Direction has certain tilt angle.
Further, the reagent reaction tube side wall is sealed with PVC film.
Further, the reagent reaction tube is PCR reagent reaction tube.
The utility model has the advantages that the present invention provides make bubble along air guide column based on bubble liquid surface tension and liquid flow dynamicthrust
The bubble filtering device that inclined direction is separated from liquid can be used for the research and development of real-time fluorescence PCR detection instrument, overcome to subtract
The loss of reaction reagent in few pipetting processes and the contradiction that bubble is also easy to produce in the slype and slype that design.
Bubble in PCR reagent can be carried out gas-liquid separation by exhaust chamber by apparatus of the present invention, guarantee that reagent can be filled out
Full of reaction chamber, which has the characteristics that compact-sized, small in size, gas-liquid separation effect is good.
Detailed description of the invention
Fig. 1 is structural schematic diagram of the invention；
Fig. 2 is exhaust chamber schematic illustration of the invention.
Specific embodiment
Further description of the technical solution of the present invention with reference to the accompanying drawings and examples.
As shown in Figure 1, a kind of Automatic-exhausting pickle reagent reaction tube applied to real-time fluorescence PCR instrument, including liquid inlet conduit
1, the preset room 2 of reagent, exhaust chamber 3, reaction chamber 5, exhaust passage 6 and outtake tube 8.Reagent reaction tube side wall is carried out with PVC film
Sealing.
The positional relationship of above-mentioned each element is as follows: liquid inlet conduit 1 is sequentially connected the preset room 2 of reagent, exhaust by intake chute
Room 3, reaction chamber 5；Exhaust chamber 3 connects outtake tube 8 by exhaust passage 6, and reaction is discharged in the gas that exhaust chamber can be assembled
Pipe.
Air guide column 4 is arranged at the bottom of exhaust chamber 3, and top is domed, and top opening connects exhaust passage 6, and opening is pasted anti-
Water ventilated membrane 7, separates liquids and gases.
4 array of air guide column that the bottom of exhaust chamber 3 has spacing to be less than 1mm, air guide column 4 have one according to liquid flow direction
Bubble is oriented to exhaust chamber 3 along air guide column by constant inclination rake angle, the power and surface tension of liquid that can use in liquid flowing
Top.
When the sample of nucleic acid of purification is pushed into reagent reaction tube from liquid inlet conduit 1, since surface tension liquid is full of narrow
Channel, by the air extrusion in channel to the preset room 2 of reagent, air in the preset room 2 of reagent with preset liquid formed bubble, gas
Bubble is in buoyancy float downward to liquid level, and subsequent sample of nucleic acid liquid enters the preset room 2 of reagent and mixes with preset liquid is reacted, simultaneously
It is full of the preset room 2 of reagent since liquid measure increases mixed liquor, enters exhaust chamber 3 from the outlet on top, when liquid enters exhaust chamber 3
Afterwards, liquid is flowed through from the gap of air guide column 4 under the promotion of water flow, but the bubble in liquid is led due to surface tension effects
Air column 4 stops, and since air guide column 4 has certain gradient, bubble is in impact of the inclined-plane of air guide column by water flow and buoyancy
Effect is moved up until liquid surface is at this time at the top of exhaust chamber 3, and since exhaust chamber 3 is domed, top will collect gas
Bubble, and the roomy liquid in bottom enters in channel from the outlet that bottom flows to exhaust chamber 3, forms an import in exhaust chamber 3 and goes out
Mouth is liquid closed, and the bubble floating rupture in fluid pressure next top is discharged by waterproof ventilated membrane 7 and exhaust passage 6,
After the sample of nucleic acid amount of entrance reaches the preset room 2 of reagent, 5 capacity summation of exhaust chamber 3 and reaction chamber plus preset liquid measure, reaction
Liquid is just filled with reaction chamber.
Using deionization water and air, the gas-liquid two-phase liquid of manufacture mixing bubble, leads in the preset room 2 of reagent of reaction tube
It crosses the liquid inlet conduit 1 in reaction tube to continue to inject the deionized water of bubble-free, by gas-liquid two-phase liquid push-in row in the preset room 2 of reagent
Gas chamber 3, continues to inject deionized water, liquid is full of reaction chamber 5, by whether depositing in liquid in microscope observing response room 5
In bubble.It tests for 10 times or more repeatedly, records experimental result such as table 1.
Table 1
From the aforegoing it can be seen that the invention can effectively filter out the bubble that diameter is greater than 0.05mm, and it is less than the bubble of 0.05mm
Detection is influenced less since size is less than fluorescence detection spot size.
Claims (5)
1. a kind of Automatic-exhausting pickle reagent reaction tube, which is characterized in that including liquid inlet conduit (1), the preset room of reagent (2), exhaust
Room (3), reaction chamber (5), exhaust passage (6) and outtake tube (8)；
The liquid inlet conduit (1) is sequentially connected the preset room of reagent (2), exhaust chamber (3), reaction chamber (5) by intake chute；The row
Gas chamber (3) passes through exhaust passage (6) connection outtake tube (8).
2. Automatic-exhausting pickle reagent reaction tube according to claim 1, which is characterized in that the bottom of the exhaust chamber (3)
There are air guide column (4), top is domed, and top opening connects exhaust passage (6), and opening pastes waterproof ventilated membrane (7).
3. Automatic-exhausting pickle reagent reaction tube according to claim 2, which is characterized in that the bottom of the exhaust chamber (3)
There is spacing to be less than air guide column (4) array of 1mm, air guide column (4) has certain tilt angle according to liquid flow direction.
4. Automatic-exhausting pickle reagent reaction tube according to claim 1, which is characterized in that the reagent reaction tube side wall is used
PVC film is sealed.
5. Automatic-exhausting pickle reagent reaction tube according to claim 1, which is characterized in that the reagent reaction tube is PCR
Reagent reaction tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811424083.1A CN109536373A (en) | 2018-11-27 | 2018-11-27 | A kind of Automatic-exhausting pickle reagent reaction tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811424083.1A CN109536373A (en) | 2018-11-27 | 2018-11-27 | A kind of Automatic-exhausting pickle reagent reaction tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109536373A true CN109536373A (en) | 2019-03-29 |
Family
ID=65850792
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811424083.1A Pending CN109536373A (en) | 2018-11-27 | 2018-11-27 | A kind of Automatic-exhausting pickle reagent reaction tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109536373A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1181026A (en) * | 1995-04-10 | 1998-05-06 | 帕克公司 | Settling device for a fluid containing liquid, gas and particulate materia, as well as a cleaning device provided herewith |
| US20090269767A1 (en) * | 2008-04-10 | 2009-10-29 | C/O Valtion Teknillinen Tutkimuskeskus | Microfluidic chip devices and their use |
| CN202409663U (en) * | 2011-08-04 | 2012-09-05 | 山东新华安得医疗用品有限公司 | Self-exhausting anti-bubble infusion set |
| US20130239557A1 (en) * | 2007-06-15 | 2013-09-19 | Choon Nam Son | Apparatus for removing exhaust gas pressure and preventing backflow of exhaust gas |
| CN203720162U (en) * | 2014-03-04 | 2014-07-16 | 刘金秀 | Novel burette |
| CN104001243A (en) * | 2014-06-14 | 2014-08-27 | 山东新华安得医疗用品有限公司 | Self-exhausting bubble prevention automatic liquid stopping precise filtering infusion apparatus |
| CN108543640A (en) * | 2018-04-02 | 2018-09-18 | 深圳市华星光电技术有限公司 | A kind of liquid discharge device |
| CN109536374A (en) * | 2018-11-27 | 2019-03-29 | 南京先进激光技术研究院 | It is a kind of with avoid bubble generate structure reagent reaction tube |
-
2018
- 2018-11-27 CN CN201811424083.1A patent/CN109536373A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1181026A (en) * | 1995-04-10 | 1998-05-06 | 帕克公司 | Settling device for a fluid containing liquid, gas and particulate materia, as well as a cleaning device provided herewith |
| US20130239557A1 (en) * | 2007-06-15 | 2013-09-19 | Choon Nam Son | Apparatus for removing exhaust gas pressure and preventing backflow of exhaust gas |
| US20090269767A1 (en) * | 2008-04-10 | 2009-10-29 | C/O Valtion Teknillinen Tutkimuskeskus | Microfluidic chip devices and their use |
| CN202409663U (en) * | 2011-08-04 | 2012-09-05 | 山东新华安得医疗用品有限公司 | Self-exhausting anti-bubble infusion set |
| CN203720162U (en) * | 2014-03-04 | 2014-07-16 | 刘金秀 | Novel burette |
| CN104001243A (en) * | 2014-06-14 | 2014-08-27 | 山东新华安得医疗用品有限公司 | Self-exhausting bubble prevention automatic liquid stopping precise filtering infusion apparatus |
| CN108543640A (en) * | 2018-04-02 | 2018-09-18 | 深圳市华星光电技术有限公司 | A kind of liquid discharge device |
| CN109536374A (en) * | 2018-11-27 | 2019-03-29 | 南京先进激光技术研究院 | It is a kind of with avoid bubble generate structure reagent reaction tube |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190329 |
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| RJ01 | Rejection of invention patent application after publication |