CN109526970A - A kind of wheat is degeneration-resistant to prolong the Yield increasing type growth regulator and its preparation method and application that declines - Google Patents
A kind of wheat is degeneration-resistant to prolong the Yield increasing type growth regulator and its preparation method and application that declines Download PDFInfo
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- CN109526970A CN109526970A CN201811420542.9A CN201811420542A CN109526970A CN 109526970 A CN109526970 A CN 109526970A CN 201811420542 A CN201811420542 A CN 201811420542A CN 109526970 A CN109526970 A CN 109526970A
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- wheat
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- yield increasing
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
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- Dispersion Chemistry (AREA)
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Abstract
Prolong the Yield increasing type growth regulator that declines the invention discloses a kind of wheat is degeneration-resistant, by the material composition of following mass percent: epiphysin 0.02~0.08%, surfactant 0.5~1.5%, pure water 98.42~99.48%, the gross mass percentage of above-mentioned substance is 100%, is the constituent of final suspending agent.The present invention also provides the preparation method of growth regulator and its in Inappropriate ADH syndrome, salt damage, drought-resistant, in seed soaking application.Growth regulator of the present invention enhances the photosynthetic capacity of plant leaf blade under abiotic stress, improves plant water regime, improves the resistance of wheat, has delayed leaf senile.Under potting and field test condition, wheat increases production 54.3% and 16% respectively.
Description
Technical field
The invention belongs to agricultural technology fields, and in particular to a kind of wheat is degeneration-resistant to prolong decline Yield increasing type growth regulator and its system
Preparation Method and application.
Background technique
The growth not only regulation by inhereditary material itself of wheat in the natural environment, also by many environmental factor shadows
It rings, such as low temperature, with high salt and arid, these abiotic stress factors seriously limit the growth and development of wheat and cause under its yield
Drop.Plant growth regulator is often widely used for coordinate plant growth and improves the anti-adversity ability of plant, and then improves and make produce
Amount.
But current plant growth promoter half-life short, absorption difference and unstable, and unit area usage amount is larger,
The wasting of resources is not only caused, the intracorporal pesticide residue of crop is also easily caused, pollution is generated to environment.
Summary of the invention
For above-mentioned problems of the prior art and defect, the purpose of the present invention is to provide a kind of effective component grains
Diameter is smaller, it is easier to which absorbed wheat is degeneration-resistant to prolong the Yield increasing type growth regulator that declines, it is intended to improve Resistance of Wheat To Adversity, realize wheat
The raising of yield.
Realizing technical solution used by foregoing invention purpose is: a kind of wheat is degeneration-resistant to prolong the Yield increasing type growth regulator that declines,
By the material composition of following mass percent:
Epiphysin 0.02~0.08%
Surfactant 0.5~1.5%
Pure water 98.42~99.48%
The gross mass percentage of above-mentioned substance is 100%；
The surfactant is tween (80).
The degeneration-resistant optimal technical scheme for prolonging the Yield increasing type growth regulator that declines of wheat of the invention, by following mass percent
Material composition:
Epiphysin 0.04~0.06%
Surfactant 0.8~1.2%
Pure water 98.74~99.16%
The gross mass percentage of above-mentioned substance is 100%；
The surfactant is tween (80).
The degeneration-resistant best-of-breed technology scheme for prolonging the Yield increasing type growth regulator that declines of wheat of the invention is by epiphysin 0.04%, tween
(80) 1%, pure water 98.96% forms.
It is a still further object of the present invention to provide the degeneration-resistant preparation method for prolonging the Yield increasing type growth regulator that declines of above-mentioned wheat, tools
Steps are as follows for body:
A) epiphysin, dehydrated alcohol, surfactant, the pure water for weighing the weight are spare；
B) epiphysin powder is dissolved in dehydrated alcohol, is stirred until homogeneous dissolution, process for preparation will be protected from light, and be made organic
Phase；
C) surfactant is dissolved in pure water, is stirred until homogeneous dissolution, and be put into ultrasound ten in supersonic wave cleaning machine
Minute, water phase is made；
D) organic phase is subjected to ultrasonic emulsification with being slowly added into water phase, addition speed is 8ml/min, ultrasonic time and
Temperature is respectively 30min, and 60 DEG C；
E) gained system is concentrated under the conditions of 40 DEG C, evaporates organic solvent (dehydrated alcohol), obtains that wheat is degeneration-resistant to be prolonged
Decline Yield increasing type growth regulator.
It is a still further object of the present invention to provide degeneration-resistant prolong of above-mentioned wheat to decline Yield increasing type growth regulator in Inappropriate ADH syndrome
Using.
Prolong Yield increasing type growth regulator answering in salt damage of declining it is a still further object of the present invention to provide above-mentioned wheat is degeneration-resistant
With.
It is a still further object of the present invention to provide degeneration-resistant prolong of above-mentioned wheat to decline Yield increasing type growth regulator in drought-resistant
Using.
It is a still further object of the present invention to provide degeneration-resistant prolong of above-mentioned wheat to decline Yield increasing type growth regulator in seed soaking
Application.
Compared with prior art, the beneficial effects of the present invention are:
1) wheat of the invention is degeneration-resistant prolongs the Yield increasing type growth regulator that declines, and is a kind of nano suspension concentrate, also known as " nanometer stream
Body " is to be uniformly dispersed to form stable high score in medium water by raw medicine pulvis not soluble in water and dispersing agent and various auxiliary agents
It dissipates, the suspension of continuous phase, hereinafter referred to as.
2) plant growth regulator of the invention is a kind of without organic solvent, and using water as decentralized medium, suspendability is good
Excellent novel form.The advantages of dosage form is that suspension is good, and effective component granularity is small, is easily absorbed；It is easy to use, not by water quality
Influence, can be with water with arbitrary proportion mixing good dispersion；Photodegradation rate reduces, and deposits stability with good, to the dirt of bad border
It contaminates less.
3) wheat of the invention is degeneration-resistant prolongs the Yield increasing type growth regulator that declines, and enhances the light of plant leaf blade under abiotic stress
Conjunction ability improves plant water regime, improves the resistance of wheat, has delayed leaf senile.Potting and field experiment item
Under part, wheat increases production 54.3% and 16% respectively.
Detailed description of the invention
Fig. 1 is the chlorophyll content in leaf blades and fluorescence parameter result figure of test example 1 of the present invention；
Fig. 2 is the blade hydrogen peroxide and mda content result figure of test example 1 of the present invention；
Fig. 3 is the foliar SOD and CAT Activity Results figure of test example 1 of the present invention；
Fig. 4 is the wheat biomass and net photosynthetic rate result figure of test example 2 of the present invention；
Fig. 5 is the chlorophyll content in leaf blades and fluorescence parameter result figure of test example 2 of the present invention；
Fig. 6 is the blade Na of test example 2 of the present invention+And K+Content results figure；
Fig. 7 is the wheat biomass and leaf r elative water content result figure of test example 3 of the present invention；
Fig. 8 is the chlorophyll content in leaf blades and fluorescence parameter result figure of test example 3 of the present invention；
Fig. 9 is the photosynthetic parameters result figure of test example 3 of the present invention；
Figure 10 is the boot leaf chlorophyll content result figure of test example 4 of the present invention；
Figure 11 is the boot leaf Net Photosynthetic Rate result figure of test example 4 of the present invention；
Figure 12 is the boot leaf fluorescence parameter result figure of test example 4 of the present invention.
Specific embodiment
Embodiment 1
A kind of degeneration-resistant preparation method for prolonging the Yield increasing type growth regulator that declines of wheat, the specific steps of which are as follows:
1. weighing 0.04g epiphysin powder to be dissolved in 50ml dehydrated alcohol, with magnetic stirrer to uniform dissolution, match
Process processed will be protected from light, and organic phase is made；
2. drawing 1ml Tween 80 to be dissolved in 98.96ml pure water, with magnetic stirrer to uniform dissolution, and it is put into
Ultrasound ten minutes in supersonic wave cleaning machine, are made water phase；
3. organic phase is slowly dropped into water phase with small syringe and carries out ultrasonic emulsification, it is 8ml/ that speed, which is added, in sample
Min, ultrasonic time and temperature are respectively 30min, and 60 DEG C；
4. (40 DEG C) are concentrated under reduced pressure in gained system Rotary Evaporators, organic solvent is evaporated, obtains that wheat is degeneration-resistant to be prolonged
Decline Yield increasing type growth regulator, hereinafter referred to as epiphysin nano suspension concentrate.
Embodiment 2
A kind of wheat is degeneration-resistant to prolong the Yield increasing type growth regulator that declines, by the material composition of following mass percent:
Epiphysin 0.02%, surfactant 1.5%, pure water 98.48%.
Embodiment 3
A kind of wheat is degeneration-resistant to prolong the Yield increasing type growth regulator that declines, by the material composition of following mass percent:
Epiphysin 0.06%, surfactant 0.5%, pure water 99.44%.
Embodiment 4
A kind of wheat is degeneration-resistant to prolong the Yield increasing type growth regulator that declines, by the material composition of following mass percent:
Epiphysin 0.08%, surfactant 1.0%, pure water 98.92%.
1 potting wheat of test example, which resists, cold prolongs the test that declines
Before sowing, seed dioxygen water sterilization is then rinsed well with clear water, is impregnated for 24 hours.Every basin kind 12, sowing are deep
Degree is 3cm, and every basin stays 6 plants after emergence, is put in outdoor, and be used for rain-proof equipped with mobile greenhouse.Potting wheat is moved in the jointing stage
Enter phjytotron, handled 3 days under 4 DEG C of cryogenic conditions, outdoor placement potting wheat is control.Relative physiologic index is measured, such as
Chlorophyll, fluorescence parameter, H2O2Deng.Experimental result is shown in Fig. 1, Fig. 2, Fig. 3.Nanometer epiphysin suspending agent used is the preparation of embodiment 1
Gained.
Fig. 1 to 3 the result shows that, under low temperature stress nanometer epiphysin handle wheat leaf blade chlorophyll content with higher
And Photochemical Efficiency, illustrate that epiphysin improves the photosynthetic capacity of plant leaf blade, has delayed the aging course of low temperature induction.Together
When, under low temperature stress, nanometer epiphysin improves foliar SOD, CAT activity, alleviates oxidative damage, improves oxidation resistance.
2 water planting wheat salt tolerance of test example prolongs the test that declines
Before sowing, seed dioxygen water sterilization is then rinsed well with clear water, then by seed point in the support for being covered with filter paper
It evapotranspires on disk and with preservative film covering to prevent moisture too fast.Pallet is put in vernalization 3 days in incubator.It, will be equal after vernalization
Even consistent seedling moves in 1/4Hoagland nutrient solution.It is grown 7 days after transplanting to water planting, the seedling of half is containing nanometer
It is cultivated in the nutrient solution of epiphysin (1 μM).Salt stress (100mM NaCl) is carried out after 3 days, salt stress 16 days.Entire incubation
The pH of middle culture solution is adjusted to 6.5 with 1M KOH or 0.1M HCl, and remains aeration status.Relative physiologic index is measured, such as
Photosynthetic, fluorescence parameter, Na+And K+Content etc..Experimental result is shown in Fig. 4, Fig. 5, Fig. 6.Nanometer epiphysin suspending agent used is embodiment
2 prepare gained.
Fig. 4 to fig. 6 the result shows that, under salt stress nanometer epiphysin handle wheat leaf blade chlorophyll with higher contain
Amount, Net Photosynthetic Rate and Photochemical Efficiency illustrates that aging occurs under salt stress for blade at this time, and handle can be with for nanometer epiphysin
The aging course for effectively delaying salt stress to induce.Meanwhile under salt stress, nanometer epiphysin significantly reduces Na in blade+Accumulation,
Effectively improve wheat salt tolerance ability.
3 potting Drought-resistance in Wheat of test example prolongs the test that declines
Before sowing, seed dioxygen water sterilization is then rinsed well with clear water, is impregnated for 24 hours.Every basin kind 12, sowing are deep
Degree is 3cm, and every basin stays 6 plants after emergence, is put in outdoor, and be used for rain-proof equipped with mobile greenhouse.It is taken off in jointing stage root application nanometer black
Then plain (100 μM) solution carries out natural drought processing, Osmotic treatment 7d to normal water supply level.Relative physiologic index is measured,
Such as photosynthetic, fluorescence parameter.Experimental result is shown in Fig. 7, Fig. 8, Fig. 9.Nanometer epiphysin suspending agent used prepares institute for embodiment 3
?.
Fig. 7 to 9 the result shows that, under drought stress nanometer epiphysin handle wheat leaf blade chlorophyll with higher contain
Amount, Net Photosynthetic Rate and Photochemical Efficiency, illustrate that epiphysin improves the photosynthetic capacity of plant leaf blade, have delayed drought-induced
Aging course.Meanwhile under drought stress, nanometer epiphysin improves blade RWC, improves plant water regime, is conducive to its dimension
Hold higher photosynthetic capacity.
4 wheat increase yield of test example tests (potting and crop field)
Potting: before sowing, seed is sterilized 15 minutes with 1% liquor natrii hypochloritis, is then rinsed well and is soaked with clear water
Kind processing.Seed soaking includes: control (deionized water), nanometer epiphysin (100 μM), and dark impregnates 72h.Every basin kind 12,
Depth of planting is 3cm, and every basin stays 6 plants after emergence, is put in outdoor, and be used for rain-proof equipped with mobile greenhouse.All pottings normally supply
Water, soil moisture content maintain 80% ± 5.Flower rear blade carries out undamaged physiological index determining, such as photosynthetic, fluorescence parameter
Deng.Seed is carried out after maturation and surveys production, sees Figure 10, Figure 11, Figure 12.
Crop field: identical seed treatment mode is used.Plot area is 5 × 10m, surveys and produces area 4m2。
Nanometer epiphysin suspending agent used is that embodiment 4 prepares gained.
Figure 10 to 12 the result shows that, the chlorophyll content of wheat leaf blade significantly improves after nanometer epiphysin seed soaking, most
Big Photochemical Efficiency and photosynthetic rate still maintain higher level.As a result illustrate, the wheat flag leaf functional period of epiphysin seed soaking
Extend, has delayed aging, this is just that the high yield of wheat is laid a good foundation.
Influence of the 1 nanometer of epiphysin of table to potting wheat yield and constituent element
Influence of the 2 nanometers of epiphysins of table to wheat yield and constituent element
By Tables 1 and 2 it is found that the wheat yield of nanometer epiphysin seed soaking obviously compares.Come from yield component
It sees, nanometer epiphysin seed soaking does not influence thousand grain weight of wheat, but significantly increases spike number and grain number per spike.Potting and big
Field increases production 54.3% and 16% respectively.
The above embodiment is only the preferred embodiment of the present invention, and the scope of protection of the present invention is not limited thereto,
The variation and replacement for any unsubstantiality that those skilled in the art is done on the basis of the present invention belong to institute of the present invention
Claimed range.
Claims (8)
1. a kind of wheat is degeneration-resistant to prolong the Yield increasing type growth regulator that declines, which is characterized in that by the material composition of following mass percent:
Epiphysin 0.02~0.08%
Surfactant 0.5~1.5%
Pure water 98.42~99.48%
The gross mass percentage of above-mentioned substance is 100%；
The surfactant is tween (80).
2. wheat according to claim 1 is degeneration-resistant to prolong the Yield increasing type growth regulator that declines, which is characterized in that by following quality hundred
Divide the material composition of ratio:
Epiphysin 0.04~0.06%
Surfactant 0.8~1.2%
Pure water 98.74~99.16%
The gross mass percentage of above-mentioned substance is 100%；
The surfactant is tween (80).
3. wheat according to claim 1 is degeneration-resistant to prolong the Yield increasing type growth regulator that declines, which is characterized in that by following quality hundred
Divide the material composition of ratio:
Epiphysin 0.04%
Tween (80) 1%
Pure water 98.96%.
4. a kind of degeneration-resistant preparation method for prolonging the Yield increasing type growth regulator that declines of wheat as described in claim 1, which is characterized in that
Specific step is as follows:
A) epiphysin, dehydrated alcohol, surfactant, the pure water for weighing the weight are spare；
B) epiphysin powder is dissolved in dehydrated alcohol, is stirred until homogeneous dissolution, process for preparation will be protected from light, and organic phase is made；
C) surfactant is dissolved in pure water, is stirred until homogeneous dissolution, and be put into ultrasound ten minutes in supersonic wave cleaning machine,
Water phase is made；
D) organic phase is subjected to ultrasonic emulsification with being slowly added into water phase, addition speed is 8ml/min, ultrasonic time and temperature
Respectively 30min, 60 DEG C；
E) gained system is concentrated under the conditions of 40 DEG C, evaporates dehydrated alcohol, obtained the degeneration-resistant Yield increasing type that declines that prolongs of wheat and grow tune
Save agent.
5. wheat described in claim 1 is degeneration-resistant to prolong application of the Yield increasing type growth regulator in Inappropriate ADH syndrome that decline.
6. wheat described in claim 1 is degeneration-resistant to prolong application of the Yield increasing type growth regulator in salt damage that decline.
Prolong application of the Yield increasing type growth regulator in drought-resistant that decline 7. wheat described in claim 1 is degeneration-resistant.
8. wheat described in claim 1 is degeneration-resistant to prolong application of the Yield increasing type growth regulator in seed soaking that decline.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811420542.9A CN109526970A (en) | 2018-11-26 | 2018-11-26 | A kind of wheat is degeneration-resistant to prolong the Yield increasing type growth regulator and its preparation method and application that declines |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811420542.9A CN109526970A (en) | 2018-11-26 | 2018-11-26 | A kind of wheat is degeneration-resistant to prolong the Yield increasing type growth regulator and its preparation method and application that declines |
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| CN109526970A true CN109526970A (en) | 2019-03-29 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110839618A (en) * | 2019-11-18 | 2020-02-28 | 浙江中环农业开发有限公司 | Nano protective agent for improving stress resistance of crops and preparation method thereof |
-
2018
- 2018-11-26 CN CN201811420542.9A patent/CN109526970A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110839618A (en) * | 2019-11-18 | 2020-02-28 | 浙江中环农业开发有限公司 | Nano protective agent for improving stress resistance of crops and preparation method thereof |
| CN110839618B (en) * | 2019-11-18 | 2022-09-13 | 浙江中环农业开发有限公司 | Nano protective agent for improving stress resistance of crops and preparation method thereof |
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